Csor.00g086820 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g086820
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptioneIF-2B GDP-GTP exchange factor subunit epsilon
LocationCsor_Chr05: 9603469 .. 9614247 (+)
RNA-Seq ExpressionCsor.00g086820
SyntenyCsor.00g086820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGCTCAGAGAAAGGGGGCATCGCGGGTTTCCGAGGACTCCGAGGAGTTGGCACGAGTTTCGTTGCAGGCAGTTCTTCTTGCCGATAGCTTTACCACTAAGTTTCGTCCCATAACGCTTGAACGACCCAAGGTATTTTCCAGATAACCGGAAGTGTGTTTCTTCGGAATTATAGTTCGTGCGTATTTCTTTCTATGTGTTCATAATTTAGCTAATAGTTCGATACTAGTTTGGAAAATTATAGCCCTTCATTCGAGGGCTGTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCCTGTTGTAAGCTCGATGTTGGCTATAATATCAAGCTGCGTGCAATTCAAGTTGTCACAGCTATATATTTACTTGCTACACAAGTTATTATATGCGAACTTCATCATTTGGTAGCAGTTAATACAACTTAAAACTTCAAAACTAGAAGCTTAGCTATGCCAATTATCTATTCTTTCGTGTTGCTTCATTTTGTATAGGCTCCTTGCTACAAGCTGATTGCATAGAGATCACGTATGAAGAACTTAAATAAGTTACAACGTTTTTGAAGCATTGAAAACTTGTATGATGCTTCTTGTTCCATTAATATTTCCCTTTTTTTAATATCTATAGGTATTACTGCCGCTGGTAAATGTCCCTATGATTAATTATGCCTTATCCTGGCTCGAATCGGCTGGTGTTGAAGAGGTGTTTGTATTTTGCTGTGCTAACTCGAAGCAAGTAATTGATTACTTGGAGAATTCTGAGTGGGTATCCCTACCAGACTTTGCAGTCACGACTATAGAGTCACACAACTCCATTAGTGCTGGAGACGCCCTGCGTTTGATCTATGAAAGAAATGTGGTACGCATGTCTGGAACTGATATATGGGACTTATTTTAATACCGGCATTCTCTATAAGAACTAAATCCTGACACATGAAATGGATTTTGAATGATGCATTTTTATCCATTCCCAAATCCAAATATTCACTCACATTTGTTTATTTATGGATTTTGAGCTGTTGATATGTTTTGTTCCACTCTCAAATCCGAAATAAACACATTTAAATATTTTCTTGAGTAACTCGGACCCATACTTGTTAATTTCATTTACTATAAGTTGATATGATCATCTCATTAGCCAAGAGCAAGAGCAATTGTTACCCTTGAAAATTGGTCCACTTAGCTATTTTTGCGAGTGTATATAATGTTAAAAAGTGATGTATGTATGGTGGCACCTCTACCTTAATGCTGTTAAGATAATCAAGAGTGCAATTTTCTAAAGAGAAAAATCAACTTTCTACTGTCACTTTTTCATTTACCACACGACTTTATAAATTTTATTTACAGTGCCAAAAAAACTGCCATAATGATTGACTTTGCACTTTCATAACCTTTATGAGTGATTCATTGTGCCATCCAGTTTGTATAGGTTGTTTCACTCAGGTGTCTTATAATACCTCTAAATTTACCCTTTTGAAGATACATGGTGATTTTGTACTTATCACTGGAGACACCATAAGCAACATGCCACTTACGCAAGCTCTTCAAGAACACAAAGATAGAAAGAAGAAAGATAGTAATGCCGTGATGACTATGGTTGTCAAACGGTCAAAGCCTTCTCCAATCACACACCAATCTCGGCTTGGCACTGATGAACTGTTTATGGCGATTGATCATAATTCTAAGCAGCTCTTATACTATGAGGATAGGGCTGACGATTCAAAAGGAATTATACATCTGGACAAGTCATTGCTAATGGATAATCCTTCCATATCACTTCATAACGACTTGCAGGTTATTTAATTTAACTTCAAGTGCTTATCTTGTTTATTTTTGTTGTTAATGTTACATCTTGAGTTATAATTTTTCATGATAAGAAGCAAATTGCATTGCTAACAAAAAATGTAACATGAAGAGGACAAGTGGTCCAAACACGAGATAGGGACAGGGGATATTACAAATTCTCCAATTAGTCTTTTCACGATGGGCTTTTTGTTGCATTTTGCATTGAAGGGTCTAAAGCTTATAAAAATATGTCTCGAATGTGTTGGAAAACTTTTTAAAAATCAGACACATCTTCACATTTCAGAATGTGTTGGACACCGACAGCTACAATAGGAATTACTGTGCATCTGGCTCCTACCTTTGTATATCTTGTTAGTAATTGATAAATGCAGGAATGGCATCGTGTCAAAAGAAAGGTAAGACTAAATCATGTTTATGTTATGCAGGATTGTTATATAGACATATGCTCCCCAGAAGTACTCAGCCTGTTTACTGACAATTTTGACTACCAACATCTACGTCGTCATTTTGTCAAGGGTTTGCTCTTAGATGACGTACGTATACCTTATTATGGACCTGTAGCCTCTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTCTTTTTAATTAGAATTTTATATCTATTAAATTAAGCTTATTTTATTTCATGAACAATGACAGCTTTTGATATATATGCTCGAATTATGACTCCAACAACATAATGAATTAATTCAATTTCTTTGTATTTTTTTTTTGTTAATTATATATTTTCATTCATTTTCAAAATTATGTGGGAAGAAAGTTTCTTAGCAGAAAAATTTATCCTACTCTTTATGTTTTTCCCATTCAAGAATGAGTAAGGTGCTTACTTCTATTAGCACGTCACTTAAAATGGTGGGAGTTAGGAATTGGTTATTTATTTATTATCATGTTTATTTTTGTGACTGAAGTTTGGTGACATTAAACCTAGCAACCTCCCAAACTTATCCCTTGATTGTATAAGATTTCATTCATTGTTCTTATTAAAAAAAAAATCTGTTTTGAGTTATTTTTGAATAAGAAACCATTTTATTGATGGCATGAAATTACTATAAAAGAGGGGAAAGCTCCCCATCCAAGGAGTTACAAAAAGCCTATCTATTGAATAAGAGAGTATTAAATTAACATAAAGACAAAAATTTTAGAATTAGTTTATGGAAATAAATGAGCAACCTTTTATATTCAAACTTGATTAATATTAAGTTTATTTGGTTAGGTTGAGAGGGAATCAAAAGTTGATACGTGAGAGGAAATACTTTATGGATGGGTGGGAGAGTGGGAAAGAGAAATGTGGAATTGAATGAGAGATAGCGTAGAGATGGGTTGGGAGAGAGAAAATGTGGAATTGGGTAGAGTGGGTGAGAGAGAAACTTGTAATAATGTTTGTTATTATAAACATTAGTTAGTGTTGGAGCTCCTTTTGGTGGGCTTGTTTTTTTATATACCCTTTTACATTATTTTATAATGCCAATATAATTATGGACAATGATTTTCAGATCATGGGTTACAAGATTTACACACACGAAATTTGCTCAAGCTATGCAGCCAGAATCGATAATTATCGAAGCTATGACACCGTTAGTAAGGACATAATACAAAGGTGGACATATCCACTGGTACCGGATGTTAAATTCTTTGGGAATTCTGCTTATAAACTTGAAAGGCAGGGAATGTATCGAGCATCAGGTAATGATCAAGTGATTAAACAAATTGCTTAAGTTTAGGCAAGGCTCGTTGATGTTTTTCTTCATGTTACTCAACGTCATCACTGGAGACCAGTATTTTCTTACCCCTGATATTATATTCTTTCCTTTGCCCTCAAAAGTTCATGAGCTTGTCTTTTTCTTCTTTGTTGCGTTGCTATGTGATGCTTTTATTATCTTCTTTTGCAATGCCAATGACGTCTCTTTTGATTTTGATCACATAATTGTTTGTTCGTTAAATGGTTCCATGGAAAAAAATTTAGCTTCTGACTATCTTGGACGAGCTTCTTTTGCCTAGCTTACTTCACGATGTCATTGCTTTCTTTTGTATGGAGGAGCCATGTTATAAACATACAATATTGTTTTTTATTCATTTAAATGAGAAACAAGAAAAGCTATATTATAAATTACAAAAGGAGAACAATCTAGCTGCCTTAGGGGGATTAGGAGGAATGGAAGAAATGGCCTCCAATCCAAGTAGACCAACAAAATAGGTGTATTACAAAAGGTTTTTTCACAAATCGATTATTATGTCAATCTCAATTCATGGGTCGAGATCCAAGTTATGCACAAGAGATATTGTGGACTTCACGAAGTTTGGGTTTGTCTTGTATGATTTCATCAATCCAATATGCAGTATGTTTAGGTTTTATAGCGAGGGTCAGTGGCTCTCATATTATTCAGGTCAAGTTTCATGTTGGTGACATTTAGTTTGCTATATTACTCTCAATGCGGCCAATTTTTTTTACCAACTTCTTTGGAGTTTTTGCTCTTCCCTTACATATTCCATTTATTAAGTGATGACATTGCGTTCTCTTCAGAAATACTGCAGGCACGCTCTGCACAGGTTGGTCCGTTTACTGTTATTGGAGATAACAGCAAAATTGGTGACAACACCAAAATTACGAATTCAATTATTGGGCAAGGTTGTAGCATTGGATCTAATGTTACAATTGAAGGCTCTTATATATGGGACAACGTCACCATTGAAGATGGCTGTAAACTTAGTCATGCTATCGTTTGTGATGGAGTTATTATAAAGTCAGGGGCAGAACTGGAACCTGGTGTAATTTTGTCTTTCAAGGTATTGTTCCTTCTTTTTGTTTATTAATGCAGCTAGTCTATTTATTGACCGTAATCAATTGAAGTGATCTATAGTACTCACGCACAGAAATTTATAATATCTGATAAAATTGGCTTCAAGAGTGTCAAATGACTTAACACTAACGACAAACGTGTTCCTTACGTTGATAATTGGCCTATGGCTGTTTACTACTCAGATGTCCTTGAAGCTGGATTGTGCCATCCATTTAATCTTTTGTATGCCCTTCTCTTTTCCCGAGTAGGAAATTGCTTTTCATTTTTGTTTTATCTCCCTAGCTTCACATTGGACGCCCTTTTTGTGGCTTGGCTTTTAGGCTTCAATTTTTCTTAGTATGAGCGTTTCTAATAAAAAAAGAACTTTTTGGATGTTGCAATTCTTTTTTTCTTTAATGAAAAACAAAGGATTTTATTCATGCAGTAAGATTCCTCAACAAAACAGCCCAAGGGACAAGGGGATAAGGCTTCCCTTTTCCGGAAGGAAGGGTTACAAAAAAACCTTTCAATCCAAGTTAATGGTTTCTAAAATGTTGTTACAAAACAACCTATCTTGAGCACTCTAACTAGAAGCCATAAACGGTATAAAATCACAAAAAGATGAAAAAGTAGCCGGCTTGAACTTATCTAATCAATATATGGTTAAAATCTTATTTTTTATAACTATAAGTTGACTTTGACTTCATATAGTTAAGTCTCTTTTCATTTCTGGTTAAAAGTTTTGTTTCATGTTCAAAACAAAAGTTTACGGCTCAGTCTTCGATGGTGTGCACTATGTATTTGTGGGAAATGTTTTAACTTTGGTCCACTCTATAAGTTTAGAATTTCAAGTGTACCCTCTATTTTTCTACTTGGTTCTTTGGTTGTCAATGAGCGAAGGTTATAAATTATATGTCAAAATGTTTCTAAAAAATGCATTATTGGATGGACATGCACTTTAAAATTATTTTCATTTGAATGGCTTAGTCAAAATGGATTGATGATTGATCCAGTGTATATCTTTTAGCTCTACAGTTCTCGAATCTCAAGGTGTTTTACTGTTATCCTATTCTTTTATTTTTGACTTAGTTGTTGGACAAGGAAAATCAATTTTTGTGCATAGGATCAAAGTCATATGAAATTGATTATTTTAAACGTGGCTAGGATGTTAGATATTAAACTTGTTTCATGCACCTATAGATACACGAATTTGTGGGATACGTGTACATGAGAGGTCCATGTACTTGGTTAATATTTGAAGAGTCATGAGAGTTGAGATTCATTTAGCAGATACATGTATTACATGTATTAAATTATTCATGTATCACTATAGTGATAGTACATGTATTAGGTAGATTCATTATTTAATATTTGATACATGTATGGTCATGGAGAATGTTCCAAAAGAACCACCTATGTAGAGTTTTTCTCAATCAACTTTGAAATATAACACATCTCTTCCTCCCCATAGCAAAAACTTTATCAACATTGGGTTGATCAAACATACAACTATGAGCAAATATGTGATGTTGTTAGGTACGGTTTTATATTTGATAAATTAAATGTAGCATTCCCACGTATATGGAGAGAAAAGATAAAGAAACCGTTTTATTAGAAGATTAAAAAAAATCATCGATTTTGTAAGACAATTTGAACCAATATATTCAATTAAATATTAAAATGAAAAATATCACGTTAATGTATTCTGGTTATGCCCTACTCTTGATATATGAACTCAACGTTTCTTGATCCTTTTTTCTTGTACCTCTGCTGACTTTTCTATTTTGGCATTACACAGGTTGTTGTAGGGGATCAGTTTATTGTTCCATCATATTCAAAGGTATCTTTGTTTCAGCAGCCAATCAATCAAGATAGCGATGATGAGCTGGAATATGCAGATAATAGCAGTGGTATAGTAGAATGTAAAACTTTTAGGCAAATACTTCCTATTCATTTTGAGCAAACTTTACTTCAACGGGCCATTTAATGTTTTCTTGATGATAAGAAACCATTTCACTGAAGGTAATGAAATTATGAAAGAGTGAGTAAGACTAGAGAGAAAGAGGTATCAAGGGTTTTCCAGGTGGCAAGAGATTTAGAATAGACGAGAGGGTGAGTGGTAAGGTGAATTTCTTCAATACAAGGAGACGAGGATCATTATGTTCATCAATTCTCTTTCTGATTTGAGGCATGGGGAGGAAGAAACAATAGAATTTCTAATGAAAAACGCTCTTCGTAGGAAGATGATTGTGATTTGACAAAAAAAAAAGAAAAAGAAAAAAAAAAGATTTATTTGGGCTGGGATGCCTTTGTGAACAACTTGGACTTTTAAATTGGTTGTTGGATTGGTATTTCGACTGAAAATTAAAGCCATTTAGTTTTAAGGTTTTTGTGTAATGAACTGCCGCAGCTTACATTACTTTGGCATATACAGTTGCAGATACAACGGACAAGTCAAATAATGAACTGATGTCCAAATCGCTGGAAATGCAAGGCTTGCCTGCATCTGAGGTTGGTATTTTTCAGATCTTCTAGCCTGATAAACAATATTAGGAATATGTCAACAAACTCAAATTGATGTAGAGAAAAAAAAAGTGGTCATTCTTTGCATAGTTCTGTCCAAGTTTTTATGCTAAATCATATGCGGGATGTCAGCTTAGAATACTAGGTAATTACAATTAATAATGTAAATGTCTCGTGCTAGGGAAGTTATAGATGTGCATTATGAGATTTTATATGGGTTCAAGGTTCATGCTTTATATCTGATCTAGATATCCCATTGAAATTTGCAGCTGATGGTAGTTTATGGTGTTTACATATCTGCCGCCAGAAGCAGTAGAAACTTGTGGAATTCCATGGTTTGATGCGAAGAGAAAATTAAATAGCTTATTTTCAAATTAGGTGCCACAAGAAGTTCTATAATACTCGTAAAGGAGCAGTGTCCTTCGCAACTTTTCTGTTTCTATTAAAAAGAATAGGTGCCATGAAAATGCCTTTGAGCACTGCTTTTCCTAGGTTGTACAAGCTTTGTAGTTGCGAGGTTCACCTTTGTCAAAAAATGCGAGCCTTGAATGGATAATGGTTGAGTCTTGCCATTTAATAAGCCTCTTAACGATAGGGAGGCTACACATTCGTCTTAATTAAACTGTTCTTTTTTTTTTTTTTTTTTTTCAAAGAAGATTTTTTGCATTTTTATATAAAGCGAAGAATGAACTGTTTTTTTTTAAAGGTCTGGAGGTTGGAGAATTTAGACTAGTTCTCTATTATGATCTTTTTGAAACTTTTTTGTAAATTTCTTCAACTAGATTATATATGTTCAAATCCTTATTGTCTTCAAAACCCCTTAGCATATGTCTTTTTTAATTAACATGCCTTCTGAATTTTTTTATTTTTAAATATTGTCCTTAACTTTATTAAGAATCAGGATAGAAACTTGTCCTTTTGGGTTTAAGTATAGGAGGGTAATCGACTCTGACATTCTCTCCTGTTATTCTCAGCTAGGCAATGGAGGTATAGGACATGTTTGGTCAATTTGTGAAGGAAGTGTTGAAGAAGAATGGAGGCACTCAGTTGCACCCATTCCTGCCGATAAGCTTGAGAAGATTCTGCAAAAAGCGACAGATGATGAGCTGGAGTTAACTCGAGATGCTAATGTTCTTCCAACTTCAGGAGAGTTGAAGTCTGACTCAAATGTCTCAGATGGTGATGATAATGAAGACTCTAGAGATGACTCCATCCACTTTGAGAAAGAGGTTAGAAGTTTTGTTTTTCTGATGGCTGGTGGCAGGGACAATCTTTTGCTTGCTAACTTCTATTAGTTTTTTATTTACAGGTTGAAGCAACGTTTCTGAGGGGTGTGCACGAAAATATTAAAGTAGACAATGTAATTTTGGAAGTGAACTCATTGCGGTATGCTATCATCTCTTTTTGTTTTTGCGGTGATTTCATGTCTTTTTCTTCTTTCCCTTTTGAATTACTTTATGTTGTTGCGTTCTGCCCAATTTCAAATTTATTTTTTGTTCAATTGATTTTATTGTATTTATGTCGCTGCTGTTGGAGATATTTTTTCAATATCACTTATTGACTGAAAAGTGTTCTCAACCTCTTAACAGACATTTTGTTGAATTCCTACATATGCAAGATGTCTGCAAGGTTGTGCCATGTAAAAGAAGATTAACGTTCTCTTTTTCTGAAAAAACAAGGATTTTCTGGTGTGAGCTTTTACGTTTGAAATCGACTCACATTTCCAAGATGTCTTCAATTTGTGTCGTCCAAGGTGCAAAAAAGATTAATATTCTTTAAAAGAGACAAAAAGTGAGATTTCCTGTTGTGAACTTTTGGTTTTGGACGCTTCAGTGACATTCTGGAGGTTGATTGTTTCATGTTTTTGCAAAATATAATTTCTTGATGACAGCTATTCTCTTTCCTTCGTTTTTTCTTTCTATATTGTTTGCTATGGCAGGCATTTTTTCGGTAAAATTATCTATTAGAGTTAAACGATATGTTAATATTTGCCGATAAAATTTTGTTTTTTCTTTAATACAATTAATCGAGTATAAGTTCGCTGCTTTTGCACTAATGGTCAGGATGTTTTGAAGTTTAAACCTAGGCTCAGTGTTTGTATACTGCTTGCTTAAATTAACTCTCTCGGCAGGTTGTCATATAATAAGGTGGCTGCAGATTGTGCAGGAGCATTGTTTTATTCTCTGATGCAATTTGCCTTAGACTCTCCACATGGTTCATCCAGTAAGTGCTTTGAGCTGTCTTTCCTTTTGGTATGGAGACTTTATGATAAGCAAAGTTTAAAAGGTTAATGTAATCGAAAACGTTAGGTTCTCATTTTTATGAAAATATTGATAGAAAGATAAAAAAAAATGTCGATCTTGATGAATATTTTTGTTATGGAAAAAAATCATAAAAAAGAAAAAAATTCAAAAGTATTTAAATTAATAAATAATCATTTACACTTGGGCTTATTGTTAATATTAAAAACACTTGGTGACATCTTATTGCTATTTACGTTTTTACGTTTCGTCAATTTTTCTACGGTATAATGGAGATCTCGAGGTTAGATCCATGAACGTATGGAAATGGACCGAAATTTAATACCTCATGATCAAGTAAAGTCTAATAATACCAAACTATGTGGTGTGATGATACATTTTGTGAAGATTTGCGTTACCTGTTAACATTTTATCATCTTGAATTTGCAATATATTATGTCGTCAATTAGTCTGCATTGGATTAGAGCTTGCAATACCATGCCTCGGTATATCAAATGGTTTGCTACCAACGTTCATCTGGAAACTGAGTTTGAAGGTTGTCGAAGAAATTGCCTTGTGAAAAACCATTCTAGCTAAATTTTTATGAAAAACTGAGTTTGAGTTTGAAGGGTTTCTTTCCTTCATGTACTCTTTGTTCCTTTTTCTTTTTCTTTTTTGAAACTGCCAAATTAATTCCATCTGTTTCAGGTGAGCTACTGCAAAACACTACAAATATAATTACGAAATGGAAAAAACTTTTGAAGTACTACTTAACTGACATGGATGAAGAGGTATTGCGAATTTTAACACTGTTAGCTGATTAACTTAACCAATATATTGTACTTTGTGCAAGTAGTTGAACAAATCCTATATCCTCTGTAGATTGAAGTGATATTAAAATTTGAAGAGATGTGCCTGGAATCGGCCAAGGAATTCACTCCACATTTTACTAAGGTATTTTTATTGTTTTCTTGGAAAATGGGTCTCTAGATTGAGAACATGCATTCCTTATTCTTAGAGGTTAATTACGTTCGATATAGATAGACTTCTAGATAATATTCGAGGCTGAAAAATGATAGTCTATCAGGATGTACCTGTATTTATGATATTTTTCCCTCAAGGGCGAGGTGAATGATTTTCATACACATTTTGTGTTCCATAGATTTTGTGTCATCTATATGACCAAGAAATCATTCAAGAAGATGCTATTCTAAGGTGGGATGATGAGAAAAAGGAAGCGGATGATTCGGATAAGCTTTTCGTTAAGCAGGCCGAGAAATTCATCGAAGTAAGGGACTTTTTACGTCCTGTTATCTGTTTCATTTTCAAATAGATCGGAACTTATTTGTAGCTTTGGTACAGTGGTTAAGAGTTGCATCAGAAGAGGAAGATGATGAAGAGGAGGAATAG

mRNA sequence

ATGGGTGCTCAGAGAAAGGGGGCATCGCGGGTTTCCGAGGACTCCGAGGAGTTGGCACGAGTTTCGTTGCAGGCAGTTCTTCTTGCCGATAGCTTTACCACTAAGTTTCGTCCCATAACGCTTGAACGACCCAAGGTATTACTGCCGCTGGTAAATGTCCCTATGATTAATTATGCCTTATCCTGGCTCGAATCGGCTGGTGTTGAAGAGGTGTTTGTATTTTGCTGTGCTAACTCGAAGCAAGTAATTGATTACTTGGAGAATTCTGAGTGGGTATCCCTACCAGACTTTGCAGTCACGACTATAGAGTCACACAACTCCATTAGTGCTGGAGACGCCCTGCGTTTGATCTATGAAAGAAATGTGATACATGGTGATTTTGTACTTATCACTGGAGACACCATAAGCAACATGCCACTTACGCAAGCTCTTCAAGAACACAAAGATAGAAAGAAGAAAGATAGTAATGCCGTGATGACTATGGTTGTCAAACGGTCAAAGCCTTCTCCAATCACACACCAATCTCGGCTTGGCACTGATGAACTGTTTATGGCGATTGATCATAATTCTAAGCAGCTCTTATACTATGAGGATAGGGCTGACGATTCAAAAGGAATTATACATCTGGACAAGTCATTGCTAATGGATAATCCTTCCATATCACTTCATAACGACTTGCAGGATTGTTATATAGACATATGCTCCCCAGAAGTACTCAGCCTGTTTACTGACAATTTTGACTACCAACATCTACGTCGTCATTTTGTCAAGGGTTTGCTCTTAGATGACATCATGGGTTACAAGATTTACACACACGAAATTTGCTCAAGCTATGCAGCCAGAATCGATAATTATCGAAGCTATGACACCGTTAGTAAGGACATAATACAAAGGTGGACATATCCACTGGTACCGGATGTTAAATTCTTTGGGAATTCTGCTTATAAACTTGAAAGGCAGGGAATGTATCGAGCATCAGAAATACTGCAGGCACGCTCTGCACAGGTTGGTCCGTTTACTGTTATTGGAGATAACAGCAAAATTGGTGACAACACCAAAATTACGAATTCAATTATTGGGCAAGGTTGTAGCATTGGATCTAATGTTACAATTGAAGGCTCTTATATATGGGACAACGTCACCATTGAAGATGGCTGTAAACTTAGTCATGCTATCGTTTGTGATGGAGTTATTATAAAGTCAGGGGCAGAACTGGAACCTGGTGTAATTTTGTCTTTCAAGGTTGTTGTAGGGGATCAGTTTATTGTTCCATCATATTCAAAGGTATCTTTGTTTCAGCAGCCAATCAATCAAGATAGCGATGATGAGCTGGAATATGCAGATAATAGCAGTGTTGCAGATACAACGGACAAGTCAAATAATGAACTGATGTCCAAATCGCTGGAAATGCAAGGCTTGCCTGCATCTGAGCTAGGCAATGGAGGTATAGGACATGTTTGGTCAATTTGTGAAGGAAGTGTTGAAGAAGAATGGAGGCACTCAGTTGCACCCATTCCTGCCGATAAGCTTGAGAAGATTCTGCAAAAAGCGACAGATGATGAGCTGGAGTTAACTCGAGATGCTAATGTTCTTCCAACTTCAGGAGAGTTGAAGTCTGACTCAAATGTCTCAGATGGTGATGATAATGAAGACTCTAGAGATGACTCCATCCACTTTGAGAAAGAGGTTGAAGCAACGTTTCTGAGGGGTGTGCACGAAAATATTAAAGTAGACAATGTAATTTTGGAAGTGAACTCATTGCGGTTGTCATATAATAAGGTGGCTGCAGATTGTGCAGGAGCATTGTTTTATTCTCTGATGCAATTTGCCTTAGACTCTCCACATGGTTCATCCAGTGAGCTACTGCAAAACACTACAAATATAATTACGAAATGGAAAAAACTTTTGAAGTACTACTTAACTGACATGGATGAAGAGATTGAAGTGATATTAAAATTTGAAGAGATGTGCCTGGAATCGGCCAAGGAATTCACTCCACATTTTACTAAGATTTTGTGTCATCTATATGACCAAGAAATCATTCAAGAAGATGCTATTCTAAGGTGGGATGATGAGAAAAAGGAAGCGGATGATTCGGATAAGCTTTTCGTTAAGCAGGCCGAGAAATTCATCGAATGGTTAAGAGTTGCATCAGAAGAGGAAGATGATGAAGAGGAGGAATAG

Coding sequence (CDS)

ATGGGTGCTCAGAGAAAGGGGGCATCGCGGGTTTCCGAGGACTCCGAGGAGTTGGCACGAGTTTCGTTGCAGGCAGTTCTTCTTGCCGATAGCTTTACCACTAAGTTTCGTCCCATAACGCTTGAACGACCCAAGGTATTACTGCCGCTGGTAAATGTCCCTATGATTAATTATGCCTTATCCTGGCTCGAATCGGCTGGTGTTGAAGAGGTGTTTGTATTTTGCTGTGCTAACTCGAAGCAAGTAATTGATTACTTGGAGAATTCTGAGTGGGTATCCCTACCAGACTTTGCAGTCACGACTATAGAGTCACACAACTCCATTAGTGCTGGAGACGCCCTGCGTTTGATCTATGAAAGAAATGTGATACATGGTGATTTTGTACTTATCACTGGAGACACCATAAGCAACATGCCACTTACGCAAGCTCTTCAAGAACACAAAGATAGAAAGAAGAAAGATAGTAATGCCGTGATGACTATGGTTGTCAAACGGTCAAAGCCTTCTCCAATCACACACCAATCTCGGCTTGGCACTGATGAACTGTTTATGGCGATTGATCATAATTCTAAGCAGCTCTTATACTATGAGGATAGGGCTGACGATTCAAAAGGAATTATACATCTGGACAAGTCATTGCTAATGGATAATCCTTCCATATCACTTCATAACGACTTGCAGGATTGTTATATAGACATATGCTCCCCAGAAGTACTCAGCCTGTTTACTGACAATTTTGACTACCAACATCTACGTCGTCATTTTGTCAAGGGTTTGCTCTTAGATGACATCATGGGTTACAAGATTTACACACACGAAATTTGCTCAAGCTATGCAGCCAGAATCGATAATTATCGAAGCTATGACACCGTTAGTAAGGACATAATACAAAGGTGGACATATCCACTGGTACCGGATGTTAAATTCTTTGGGAATTCTGCTTATAAACTTGAAAGGCAGGGAATGTATCGAGCATCAGAAATACTGCAGGCACGCTCTGCACAGGTTGGTCCGTTTACTGTTATTGGAGATAACAGCAAAATTGGTGACAACACCAAAATTACGAATTCAATTATTGGGCAAGGTTGTAGCATTGGATCTAATGTTACAATTGAAGGCTCTTATATATGGGACAACGTCACCATTGAAGATGGCTGTAAACTTAGTCATGCTATCGTTTGTGATGGAGTTATTATAAAGTCAGGGGCAGAACTGGAACCTGGTGTAATTTTGTCTTTCAAGGTTGTTGTAGGGGATCAGTTTATTGTTCCATCATATTCAAAGGTATCTTTGTTTCAGCAGCCAATCAATCAAGATAGCGATGATGAGCTGGAATATGCAGATAATAGCAGTGTTGCAGATACAACGGACAAGTCAAATAATGAACTGATGTCCAAATCGCTGGAAATGCAAGGCTTGCCTGCATCTGAGCTAGGCAATGGAGGTATAGGACATGTTTGGTCAATTTGTGAAGGAAGTGTTGAAGAAGAATGGAGGCACTCAGTTGCACCCATTCCTGCCGATAAGCTTGAGAAGATTCTGCAAAAAGCGACAGATGATGAGCTGGAGTTAACTCGAGATGCTAATGTTCTTCCAACTTCAGGAGAGTTGAAGTCTGACTCAAATGTCTCAGATGGTGATGATAATGAAGACTCTAGAGATGACTCCATCCACTTTGAGAAAGAGGTTGAAGCAACGTTTCTGAGGGGTGTGCACGAAAATATTAAAGTAGACAATGTAATTTTGGAAGTGAACTCATTGCGGTTGTCATATAATAAGGTGGCTGCAGATTGTGCAGGAGCATTGTTTTATTCTCTGATGCAATTTGCCTTAGACTCTCCACATGGTTCATCCAGTGAGCTACTGCAAAACACTACAAATATAATTACGAAATGGAAAAAACTTTTGAAGTACTACTTAACTGACATGGATGAAGAGATTGAAGTGATATTAAAATTTGAAGAGATGTGCCTGGAATCGGCCAAGGAATTCACTCCACATTTTACTAAGATTTTGTGTCATCTATATGACCAAGAAATCATTCAAGAAGATGCTATTCTAAGGTGGGATGATGAGAAAAAGGAAGCGGATGATTCGGATAAGCTTTTCGTTAAGCAGGCCGAGAAATTCATCGAATGGTTAAGAGTTGCATCAGAAGAGGAAGATGATGAAGAGGAGGAATAG

Protein sequence

MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYALSWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYERNVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTDELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLSLFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWTYPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGQGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQFIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGLPASELGNGGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGELKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAGALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEEEDDEEEE
Homology
BLAST of Csor.00g086820 vs. ExPASy Swiss-Prot
Match: Q54RF3 (Translation initiation factor eIF-2B subunit epsilon OS=Dictyostelium discoideum OX=44689 GN=eif2b5 PE=3 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 8.4e-126
Identity = 259/731 (35.43%), Postives = 425/731 (58.14%), Query Frame = 0

Query: 4   QRKGASRVSE-DSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYALSW 63
           ++ G+S + +  SE++    LQAV+L DSF  KF PITLE+P+ LLPLVN+P+++Y L +
Sbjct: 9   KQSGSSNMDQLKSEDI----LQAVVLGDSFDRKFAPITLEKPRTLLPLVNIPLLDYTLEF 68

Query: 64  LESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYERNV 123
           L ++GV+++FVFCCA++ Q+ +Y+++S W  LP   V  +   N  + GDALR +Y+  V
Sbjct: 69  LAASGVQQIFVFCCAHASQIKEYIQSSRWHDLPGVQVICMTGSNCRTTGDALRGVYDAQV 128

Query: 124 IHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTDEL 183
           I  DF+LI+GD +SNM L +ALQ HKDR++ D N +MTMV K++     TH++R   D+ 
Sbjct: 129 IQSDFILISGDVVSNMNLQKALQIHKDRRELDKNNIMTMVYKQASS---THRTRSKQDDT 188

Query: 184 FMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLSLF 243
            +  + ++ Q++ Y++     K  I ++  L   +PSI +  DL DC+IDICSPEVL+LF
Sbjct: 189 VIWCNRDTMQVVCYDNSPSKKKSSISVE--LFQKHPSIQMRYDLIDCHIDICSPEVLALF 248

Query: 244 TDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWTYP 303
            DNFD+  +R+ F+  +L  D++ YK+  + +   YAAR+ + R+Y +VSKDII RWT+P
Sbjct: 249 NDNFDFADIRKDFIHDILTSDLLDYKLSAYVLQGEYAARVKDLRTYHSVSKDIIHRWTFP 308

Query: 304 LVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGQG 363
           +VPD  F  NS+Y L RQ +Y+   +       +   TVIG  ++IG  + +++S IG+ 
Sbjct: 309 MVPDNNFMCNSSYSLSRQMIYKEKNVKLLGDCLISDETVIGTQTEIGAGSIVSHSTIGRN 368

Query: 364 CSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQFI 423
           C IG NV I GSYIWD+VTI+D   + H+I+C+G IIKS + +  G I+ F V +G    
Sbjct: 369 CIIGKNVKINGSYIWDDVTIQDNAIIDHSIICNGSIIKSSSIIGRGSIIGFNVYIGQSKT 428

Query: 424 VPSYSKVSLFQQPINQDSDDE---LEYADNSSVADTTDKSNNELMSKSLEMQGLPASELG 483
           +  +SK+++ Q   N+D DDE    EY    ++ D  + +NN     +   + L  +EL 
Sbjct: 429 LEPFSKITMAQ--YNEDEDDEELLEEYFKEINLNDDNNNNNNNNNENNKTNRWLMENELY 488

Query: 484 NGGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGELKS 543
           N  +  +      S+ ++     +    DK                       + G++K+
Sbjct: 489 NELVPRI----NDSIHDDIESDESGDEGDK-----------------------SGGKIKN 548

Query: 544 DSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCA 603
           + N    +D+     DS+ F +EV  T  RG+ E + ++N+ LE+N L+ +Y++   DC 
Sbjct: 549 NKN----NDDNPIEPDSVKFHREVGDTIRRGIIEKLPLENIQLEINGLKFAYDRDGLDCL 608

Query: 604 GALFYSLMQFALDSPHGSSS----ELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEE 663
            ++   L++ +  S   + S    EL Q     I+ +  LL  + ++ D  +++I K ++
Sbjct: 609 TSILPVLLESSSSSSSTTDSVTPKELQQFIAGRISAFSPLLVKFSSE-DSMVDLIFKIQD 668

Query: 664 MCLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLR 723
            C E+ K F   F  IL  LY+ ++I E+AI  W +E +  ++ D  ++K+ + FI+WL+
Sbjct: 669 FCDENEK-FKVVFQPILHQLYENDVISEEAIFEWAEEIEGDEEDDGFYLKKCKGFIDWLK 695

Query: 724 VASEEEDDEEE 727
            A EE DD ++
Sbjct: 729 SAEEESDDSDD 695

BLAST of Csor.00g086820 vs. ExPASy Swiss-Prot
Match: P56287 (Probable translation initiation factor eIF-2B subunit epsilon OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tif225 PE=1 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 8.5e-110
Identity = 231/714 (32.35%), Postives = 395/714 (55.32%), Query Frame = 0

Query: 17  ELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYALSWLESAGVEEVFVFCC 76
           E  + +LQA++L+DS+  +FRP+TL++P+ LLPL N P+I Y   +L  AGV+EV+VFCC
Sbjct: 12  EKPKHALQAIVLSDSYNYRFRPLTLDKPRCLLPLANTPLIEYTFEFLALAGVQEVYVFCC 71

Query: 77  ANSKQVIDYLENSEWVSLPD--FAVTTIESHNSISAGDALRLIYERNVIHGDFVLITGDT 136
           A++ Q+ +Y+E S+W +LP   F+V TI S  S+S GDALR +  + +I  DF+L++GD 
Sbjct: 72  AHAGQIREYIEKSKW-NLPSSPFSVNTIVSRESLSVGDALRELDSKQLITSDFILVSGDV 131

Query: 137 ISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTDELFMAIDHNSKQLL 196
           +SN+PL + L+EH+ R++ D NA+MTMVV+ + P    H++R  T+     ID  + Q +
Sbjct: 132 VSNVPLNEVLKEHRKRREDDKNAIMTMVVREASP---FHRTRARTESSVFVIDKKTSQCV 191

Query: 197 YYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLSLFTDNFDYQHLRRH 256
           +Y+   +  K  + +D  +  ++  + + NDL DC IDICS +V +LFT+NFDYQ +R+ 
Sbjct: 192 HYQAN-ERGKHYVSMDPEIFNEHEELEVRNDLIDCQIDICSNDVPALFTENFDYQDIRKD 251

Query: 257 FVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWTYPLVPDVKFFGNSA 316
           FV G+L  D++G KI+ H    +YAAR+ + ++YD +SKD++ RW YP VPD     N  
Sbjct: 252 FVYGVLTSDLLGKKIHCHVAKENYAARVRSLQTYDAISKDVLSRWVYPFVPDSNLL-NQT 311

Query: 317 YKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGQGCSIGSNVTIEGS 376
           +  +R  +Y+  +++ ARS  +   T+IG  +K+GD + + N+IIG+ C+IGSN +I+ +
Sbjct: 312 FSYQRHQIYKEEDVVLARSCIIKARTLIGAYTKVGDASVVANTIIGRNCTIGSNCSIDSA 371

Query: 377 YIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQFIVPSYSKVSLFQQ 436
           ++W++V I D C++  AI+ + V I +   +E G I++  VV+GD  I+    +++ F+ 
Sbjct: 372 FLWEDVVIGDNCRIGKAILANSVKIGNNCSIEDGAIVAAGVVIGDNTIIEKNKRLTTFE- 431

Query: 437 PINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGLPASELGNGGIGHVWSICEGSV 496
                                   S   L   SL         +G GG G  +   E S 
Sbjct: 432 ----------------------SHSQGTLNDPSL---------VGIGGRGQEYHAEEDSD 491

Query: 497 EE-EWRHSVAPIPA-DKLEKILQKATDDELELTRDANVLPTSGELKSDSNVSDGDDNEDS 556
           +E E+  +   I + ++L     ++++       D   +P S    S + ++  D +E  
Sbjct: 492 DEGEFMEASGLIESTNELHLSDSESSETSSSSEEDMEFIPFSARRDSANTINSEDFDEGD 551

Query: 557 RDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAGALFYSLMQFALD 616
                 F KE + +  R   EN ++D   LE+N+LR++ N    +   A+  +L++  + 
Sbjct: 552 ------FNKEAQQSLERAFEENHQIDIAALELNTLRMAMNANYHEVRSAIVLALLRRIMH 611

Query: 617 SPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLESAKEFTPHFTKIL 676
                   L      ++T+W  LL       +E+++ +L  ++ C+  +   T HF ++L
Sbjct: 612 LDVSPKEAL----AKVMTRWGPLLAKLTFSHEEQVDNVLTLQKYCVRLS--MTRHFLQLL 671

Query: 677 CHLYDQEIIQEDAILRW-DDEKKEADDSDKLFVKQAEKFIEWLRVASEEEDDEE 726
            + Y  EI +E+AI  W  D +    +   L     ++F++WL  A  E + EE
Sbjct: 672 GYFYQLEIAEENAIQEWYSDPRSSEGELAALRDAGGKQFVDWLNTAESESESEE 675

BLAST of Csor.00g086820 vs. ExPASy Swiss-Prot
Match: P87163 (Translation initiation factor eIF-2B subunit epsilon OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=GCD6 PE=3 SV=3)

HSP 1 Score: 395.6 bits (1015), Expect = 1.2e-108
Identity = 250/744 (33.60%), Postives = 406/744 (54.57%), Query Frame = 0

Query: 12  SEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYALSWLESAGVEEV 71
           S+ S++L     QA++L DSF T+F P+T   P+ LLPL NVP+I Y L +L +AGV EV
Sbjct: 15  SKKSKDLVDERFQAIVLTDSFETRFMPLTAVHPRCLLPLANVPLIEYTLEFLANAGVNEV 74

Query: 72  FVFCCANSKQVIDYLENSEWV-SLPDFAVTTIESHNSISAGDALRLIYERNVIHGDFVLI 131
           ++ C A++ Q+ +Y+ENS+W+     F+VTTI S  S S GD +R +  R +I GDF+L+
Sbjct: 75  YLMCSAHADQIQEYIENSKWMGDNSPFSVTTIMSIESRSVGDTMRDLDNRGLIAGDFLLV 134

Query: 132 TGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTDELFMAIDHNS 191
           +GD ++NM  ++ALQ HK +K +D + + TMV+ ++ P    H++R   D     +D  +
Sbjct: 135 SGDVVTNMDFSKALQFHKQKKAQDKDHIATMVLNQASP---LHRTRSQIDPAAFVLDKET 194

Query: 192 KQLLYYED--RADDSKGIIHLDKSLLMD-NPSISLHNDLQDCYIDICSPEVLSLFTDNFD 251
            + ++Y+        K  I +D  LL D    + + NDL DC++DICSP V  +F +NFD
Sbjct: 195 NRCIFYQSIPPVSGKKTCISIDPELLEDFQGELQVRNDLIDCHVDICSPHVPQIFQENFD 254

Query: 252 YQHLRRHFVKGLLLDDIMGYKIYTH--EICSSYAARIDNYRSYDTVSKDIIQRWTYPLVP 311
           YQ+LR  F+KG+L  D++   IY +  +  S YAAR++++ +YD +S+DI+ RW YPLVP
Sbjct: 255 YQYLRSDFLKGVLTSDLLKKTIYAYISKDSSEYAARVESWSTYDAISQDILARWCYPLVP 314

Query: 312 DVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGQGCSI 371
           D      ++Y  E   +Y+  +I+ A+S ++G  T IG NS +G+ T+I NS+IG+ C+I
Sbjct: 315 DSNLVEGNSYSYELNNIYKEDKIILAQSCKIGTSTSIGRNSSVGEGTQIKNSVIGRNCTI 374

Query: 372 GSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQFIVPS 431
           G NV IE SYIWDN  I+D   L+ +IV     I +   L PG ++ F V++GD  ++P 
Sbjct: 375 GKNVVIENSYIWDNAVIKDNSVLNRSIVAADAQIGNNVTLSPGSVIGFNVIIGDDKVIP- 434

Query: 432 YSKVSLFQQPI-----------NQDSDDELEYADNSSV-------ADTTDKSNNELMSKS 491
              V + + PI             +S+DE EY D ++V        +   ++      +S
Sbjct: 435 -HNVKIVETPIVTENEFGDFDDESNSEDENEYEDGNAVPVLAVKDVELVGETGKGFAYES 494

Query: 492 LEMQGLPASE--LGNGGI-GHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELEL 551
               G    E  +GNG   G ++ +   +V ++   S+A +   K++K   +       L
Sbjct: 495 EIESGDEDDEEFVGNGTYSGIIYQMKSLNVSDD---SIASVSNKKVKKHSHRR-----RL 554

Query: 552 TRDANVLPTSGELKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEV 611
           + ++ +    G  +SD    +G++ ED       F  E  AT  R +  N  +D  +LE+
Sbjct: 555 SMNSMISDNGGAFESD----EGEEEED-------FGVEGLATVTRAIENNHDIDTALLEL 614

Query: 612 NSLRLSYNKVAADCAGALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMD 671
           N+LR+S N    D       +L+   +D     +    +  T I TKW  + K  +   +
Sbjct: 615 NTLRMSMNVTYHDVRSVTTQALVNKIVDFITTGTLTPQEAATKIFTKWGIMFKRQVFSPE 674

Query: 672 EEIEVILKFEEMCLESAKEFTPHFTKI-LCHLYDQEIIQEDAILRWDDEKKEADDSDKLF 728
           EE++++   EE      K +      + +   YD E+++E+ IL+W ++  E D+   L 
Sbjct: 675 EEVDLLNIVEEKSSVLDKAYNQIVLFLGVKSFYDMEVVEEENILKWWND-GENDEVRTL- 729

BLAST of Csor.00g086820 vs. ExPASy Swiss-Prot
Match: Q64350 (Translation initiation factor eIF-2B subunit epsilon OS=Rattus norvegicus OX=10116 GN=Eif2b5 PE=1 SV=2)

HSP 1 Score: 377.1 bits (967), Expect = 4.5e-103
Identity = 240/733 (32.74%), Postives = 394/733 (53.75%), Query Frame = 0

Query: 12  SEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYALSWLESAGVEEV 71
           ++ +EE     LQAVL+ADSF  +F PI+ ++P+VLLPL NV +I+Y L +L + GV+E 
Sbjct: 27  TQGAEEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQET 86

Query: 72  FVFCCANSKQVIDYLENSEW---VSLPDFAVTTIESHNSISAGDALRLIYERNVIHGDFV 131
           FVFCC  + Q+ ++L+ S+W    SL    + T + + S+  GD LR +  + ++  DF+
Sbjct: 87  FVFCCWKAAQIKEHLQKSKWCHPTSLNVVRIITSDLYRSL--GDVLRDVDAKALVRSDFL 146

Query: 132 LITGDTISNMPLTQALQEHKDRKKKDSN-AVMTMVVKRSKPSPITHQSRLGTDELFMAID 191
           LI GD +SN+ +++AL+EH+ R+K + N +VMTMV K S PS   H +R   D + +A+D
Sbjct: 147 LIYGDVVSNINISKALEEHRLRRKLEKNVSVMTMVFKESSPS---HPTRCHEDNVVLAVD 206

Query: 192 HNSKQLLYYEDRADDSKGIIHLDKSLLMDNPS---ISLHNDLQDCYIDICSPEVLSLFTD 251
             + ++L+++     ++G+ H    L +   S   + +  DL DC+I ICSP+V  LFTD
Sbjct: 207 STTNRILHFQ----KTQGLRHFSFPLGLFQGSLDGVEIRYDLLDCHISICSPQVAQLFTD 266

Query: 252 NFDYQHLRRHFVKGLLL-DDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWTYPL 311
           NFDYQ  R  FV+GLL+ ++I+G +I+ H     Y AR+ N   Y  V  D+I+RW YPL
Sbjct: 267 NFDYQ-TRDDFVRGLLVNEEILGNQIHLHVTSREYGARVSNLHMYSAVCADVIRRWVYPL 326

Query: 312 VPDVKFFGNS--AYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGQ 371
            P+V F  +S  +Y   R  +YR  E+     + +    ++G  + +G N  ITNS+IG 
Sbjct: 327 TPEVNFTDSSTQSYTHSRHNIYRGPEVSLGHGSVLEENVLLGAGTVVGSNCSITNSVIGP 386

Query: 372 GCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQF 431
            C IG NV ++ +Y+W  V +  G ++  +++CD   +K    L+P  +L+ +VVVG   
Sbjct: 387 NCHIGDNVVLDQAYLWQGVRVAAGAQIHQSLLCDRAEVKERVILKPHCVLTSQVVVGPDI 446

Query: 432 IVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGLPASELGNG 491
           I+P  S +SL      +D DD  +++D+S      +K         ++++G   +E+G  
Sbjct: 447 ILPEGSVISLHPPDAEEDEDDG-QFSDDSGADQEKEK---------VKLKGYNPAEVGPE 506

Query: 492 GIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGELKSDS 551
           G G++W                     K E + +K  D+EL  +    ++    E +++S
Sbjct: 507 GQGYLW---------------------KAEDVDEK-EDEELRQSLWGLMINMEEESETES 566

Query: 552 NVSDGDDNEDSR------DDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVA 611
             S   +  DSR      DD   F+ EV  T  RG  ENI  DN++LE+NSL+ +YN   
Sbjct: 567 ERSVDPEELDSRAGSPQLDDIRVFQNEVLGTLQRGREENISCDNLVLEINSLKYAYNISL 626

Query: 612 ADCAGALFYSLMQFALDSPHG--SSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKF 671
            +    L + +++F L    G    +        ++  W  + + Y+    + +E +   
Sbjct: 627 KEVMQVLSHVVLEFPLQQVDGVLDPNRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAI 686

Query: 672 EEMCLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFV-KQAEKFIE 726
           E+  LE  +   P   K+L   Y  EI+ E+ IL W  ++   D   +L   +Q ++FI+
Sbjct: 687 EDFFLEH-ETLVPSLAKVLMAFYQLEILAEETILSWFSQRDITDKGQQLRKNQQLQRFIQ 716

BLAST of Csor.00g086820 vs. ExPASy Swiss-Prot
Match: Q13144 (Translation initiation factor eIF-2B subunit epsilon OS=Homo sapiens OX=9606 GN=EIF2B5 PE=1 SV=3)

HSP 1 Score: 376.3 bits (965), Expect = 7.7e-103
Identity = 238/725 (32.83%), Postives = 386/725 (53.24%), Query Frame = 0

Query: 15  SEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYALSWLESAGVEEVFVF 74
           +EE     LQAVL+ADSF  +F PI+ ++P+VLLPL NV +I+Y L +L + GV+E FVF
Sbjct: 35  AEEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVF 94

Query: 75  CCANSKQVIDYLENSEW---VSLPDFAVTTIESHNSISAGDALRLIYERNVIHGDFVLIT 134
           CC  + Q+ ++L  S+W    SL    + T E + S+  GD LR +  + ++  DF+L+ 
Sbjct: 95  CCWKAAQIKEHLLKSKWCRPTSLNVVRIITSELYRSL--GDVLRDVDAKALVRSDFLLVY 154

Query: 135 GDTISNMPLTQALQEHKDRKKKDSN-AVMTMVVKRSKPSPITHQSRLGTDELFMAIDHNS 194
           GD ISN+ +T+AL+EH+ R+K + N +VMTM+ K S PS   H +R   D + +A+D  +
Sbjct: 155 GDVISNINITRALEEHRLRRKLEKNVSVMTMIFKESSPS---HPTRCHEDNVVVAVDSTT 214

Query: 195 KQLLYYEDRADDSKGIIHLDKSLLM---DNPSISLHNDLQDCYIDICSPEVLSLFTDNFD 254
            ++L+++     ++G+      L +    +  + +  DL DC+I ICSP+V  LFTDNFD
Sbjct: 215 NRVLHFQ----KTQGLRRFAFPLSLFQGSSDGVEVRYDLLDCHISICSPQVAQLFTDNFD 274

Query: 255 YQHLRRHFVKGLLL-DDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWTYPLVPD 314
           YQ  R  FV+GLL+ ++I+G +I+ H     Y AR+ N   Y  V  D+I+RW YPL P+
Sbjct: 275 YQ-TRDDFVRGLLVNEEILGNQIHMHVTAKEYGARVSNLHMYSAVCADVIRRWVYPLTPE 334

Query: 315 VKFFGNSAYKL--ERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGQGCS 374
             F  ++       R  +YR  E+     + +    ++G  + IG N  ITNS+IG GC 
Sbjct: 335 ANFTDSTTQSCTHSRHNIYRGPEVSLGHGSILEENVLLGSGTVIGSNCFITNSVIGPGCH 394

Query: 375 IGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQFIVP 434
           IG NV ++ +Y+W  V +  G ++  +++CD   +K    L+P  +L+ +VVVG    +P
Sbjct: 395 IGDNVVLDQTYLWQGVRVAAGAQIHQSLLCDNAEVKERVTLKPRSVLTSQVVVGPNITLP 454

Query: 435 SYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGLPASELGNGGIG 494
             S +SL      +D DD  E++D+S      DK         ++M+G   +E+G  G G
Sbjct: 455 EGSVISLHPPDAEEDEDDG-EFSDDSGADQEKDK---------VKMKGYNPAEVGAAGKG 514

Query: 495 HVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGELKSDSNVS 554
           ++W     ++EEE             E++ Q     ++ +  ++    +  E   DS   
Sbjct: 515 YLWKAAGMNMEEE-------------EELQQNLWGLKINMEEESE---SESEQSMDSEEP 574

Query: 555 DGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAGALFY 614
           D        DD   F+ EV  T  RG  ENI  DN++LE+NSL+ +YN    +    L +
Sbjct: 575 DSRGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNISLKEVMQVLSH 634

Query: 615 SLMQFAL---DSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLESA 674
            +++F L   DSP   SS        ++  W  + + Y+    + +E +   E+  LE  
Sbjct: 635 VVLEFPLQQMDSPL-DSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEH- 694

Query: 675 KEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFV-KQAEKFIEWLRVASEE 726
           +       K+L   Y  EI+ E+ IL W  ++   D   +L   +Q ++FI+WL+ A EE
Sbjct: 695 EALGISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLKEAEEE 721

BLAST of Csor.00g086820 vs. NCBI nr
Match: KAG6599283.1 (hypothetical protein SDJN03_09061, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1426 bits (3692), Expect = 0.0
Identity = 727/727 (100.00%), Postives = 727/727 (100.00%), Query Frame = 0

Query: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60
           MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL
Sbjct: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120
           SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER
Sbjct: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD
Sbjct: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180

Query: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240
           ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS
Sbjct: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG
Sbjct: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360

Query: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ 420
           QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ
Sbjct: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ 420

Query: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGLPASELGN 480
           FIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGLPASELGN
Sbjct: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGLPASELGN 480

Query: 481 GGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGELKSD 540
           GGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGELKSD
Sbjct: 481 GGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGELKSD 540

Query: 541 SNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAG 600
           SNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAG
Sbjct: 541 SNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAG 600

Query: 601 ALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLES 660
           ALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLES
Sbjct: 601 ALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLES 660

Query: 661 AKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEE 720
           AKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEE
Sbjct: 661 AKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEE 720

Query: 721 EDDEEEE 727
           EDDEEEE
Sbjct: 721 EDDEEEE 727

BLAST of Csor.00g086820 vs. NCBI nr
Match: XP_022946813.1 (translation initiation factor eIF-2B subunit epsilon-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1424 bits (3685), Expect = 0.0
Identity = 725/727 (99.72%), Postives = 727/727 (100.00%), Query Frame = 0

Query: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60
           MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL
Sbjct: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120
           SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER
Sbjct: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD
Sbjct: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180

Query: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240
           ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS
Sbjct: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG
Sbjct: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360

Query: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ 420
           QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ
Sbjct: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ 420

Query: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGLPASELGN 480
           FIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGLPASELGN
Sbjct: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGLPASELGN 480

Query: 481 GGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGELKSD 540
           GGIGHVWSICEGSVEEEWRHSVAPIPAD+LEKILQKATDDELELTRDANVLPTSGELKSD
Sbjct: 481 GGIGHVWSICEGSVEEEWRHSVAPIPADRLEKILQKATDDELELTRDANVLPTSGELKSD 540

Query: 541 SNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAG 600
           SNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAG
Sbjct: 541 SNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAG 600

Query: 601 ALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLES 660
           ALFYSLM+FALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLES
Sbjct: 601 ALFYSLMKFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLES 660

Query: 661 AKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEE 720
           AKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEE
Sbjct: 661 AKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEE 720

Query: 721 EDDEEEE 727
           EDDEEEE
Sbjct: 721 EDDEEEE 727

BLAST of Csor.00g086820 vs. NCBI nr
Match: XP_022999250.1 (translation initiation factor eIF-2B subunit epsilon-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1417 bits (3669), Expect = 0.0
Identity = 723/727 (99.45%), Postives = 724/727 (99.59%), Query Frame = 0

Query: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60
           MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL
Sbjct: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120
           SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER
Sbjct: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD
Sbjct: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180

Query: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240
           ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS
Sbjct: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG
Sbjct: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360

Query: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ 420
           QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELE GVILSFKVVVGDQ
Sbjct: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEAGVILSFKVVVGDQ 420

Query: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGLPASELGN 480
           FIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQG PASELGN
Sbjct: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGWPASELGN 480

Query: 481 GGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGELKSD 540
           GGIGHVWSICEGSVEEEWRHSVAPIPADKLEK LQKATDDELELTRDANVLPTSGELKSD
Sbjct: 481 GGIGHVWSICEGSVEEEWRHSVAPIPADKLEKTLQKATDDELELTRDANVLPTSGELKSD 540

Query: 541 SNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAG 600
           SNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAG
Sbjct: 541 SNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAG 600

Query: 601 ALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLES 660
           ALFYSLM+FALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLES
Sbjct: 601 ALFYSLMKFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLES 660

Query: 661 AKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEE 720
           AKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEE
Sbjct: 661 AKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEE 720

Query: 721 EDDEEEE 727
           EDDEEEE
Sbjct: 721 EDDEEEE 727

BLAST of Csor.00g086820 vs. NCBI nr
Match: XP_022946812.1 (translation initiation factor eIF-2B subunit epsilon-like isoform X1 [Cucurbita moschata] >KAG7030281.1 hypothetical protein SDJN02_08628 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1416 bits (3665), Expect = 0.0
Identity = 724/731 (99.04%), Postives = 726/731 (99.32%), Query Frame = 0

Query: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60
           MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL
Sbjct: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120
           SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER
Sbjct: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD
Sbjct: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180

Query: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240
           ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS
Sbjct: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG
Sbjct: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360

Query: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ 420
           QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ
Sbjct: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ 420

Query: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSS----VADTTDKSNNELMSKSLEMQGLPAS 480
           FIVPSYSKVSLFQQPINQDSDDELEYADNSS     ADTTDKSNNELMSKSLEMQGLPAS
Sbjct: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSSGIVEFADTTDKSNNELMSKSLEMQGLPAS 480

Query: 481 ELGNGGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGE 540
           ELGNGGIGHVWSICEGSVEEEWRHSVAPIPAD+LEKILQKATDDELELTRDANVLPTSGE
Sbjct: 481 ELGNGGIGHVWSICEGSVEEEWRHSVAPIPADRLEKILQKATDDELELTRDANVLPTSGE 540

Query: 541 LKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAA 600
           LKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAA
Sbjct: 541 LKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAA 600

Query: 601 DCAGALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEM 660
           DCAGALFYSLM+FALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEM
Sbjct: 601 DCAGALFYSLMKFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEM 660

Query: 661 CLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRV 720
           CLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRV
Sbjct: 661 CLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRV 720

Query: 721 ASEEEDDEEEE 727
           ASEEEDDEEEE
Sbjct: 721 ASEEEDDEEEE 731

BLAST of Csor.00g086820 vs. NCBI nr
Match: XP_022999249.1 (translation initiation factor eIF-2B subunit epsilon-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1410 bits (3649), Expect = 0.0
Identity = 722/731 (98.77%), Postives = 723/731 (98.91%), Query Frame = 0

Query: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60
           MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL
Sbjct: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120
           SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER
Sbjct: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD
Sbjct: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180

Query: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240
           ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS
Sbjct: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG
Sbjct: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360

Query: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ 420
           QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELE GVILSFKVVVGDQ
Sbjct: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEAGVILSFKVVVGDQ 420

Query: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSS----VADTTDKSNNELMSKSLEMQGLPAS 480
           FIVPSYSKVSLFQQPINQDSDDELEYADNSS     ADTTDKSNNELMSKSLEMQG PAS
Sbjct: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSSGIVEFADTTDKSNNELMSKSLEMQGWPAS 480

Query: 481 ELGNGGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGE 540
           ELGNGGIGHVWSICEGSVEEEWRHSVAPIPADKLEK LQKATDDELELTRDANVLPTSGE
Sbjct: 481 ELGNGGIGHVWSICEGSVEEEWRHSVAPIPADKLEKTLQKATDDELELTRDANVLPTSGE 540

Query: 541 LKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAA 600
           LKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAA
Sbjct: 541 LKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAA 600

Query: 601 DCAGALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEM 660
           DCAGALFYSLM+FALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEM
Sbjct: 601 DCAGALFYSLMKFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEM 660

Query: 661 CLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRV 720
           CLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRV
Sbjct: 661 CLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRV 720

Query: 721 ASEEEDDEEEE 727
           ASEEEDDEEEE
Sbjct: 721 ASEEEDDEEEE 731

BLAST of Csor.00g086820 vs. ExPASy TrEMBL
Match: A0A6J1G4V4 (eIF-2B GDP-GTP exchange factor subunit epsilon OS=Cucurbita moschata OX=3662 GN=LOC111450765 PE=3 SV=1)

HSP 1 Score: 1424 bits (3685), Expect = 0.0
Identity = 725/727 (99.72%), Postives = 727/727 (100.00%), Query Frame = 0

Query: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60
           MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL
Sbjct: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120
           SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER
Sbjct: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD
Sbjct: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180

Query: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240
           ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS
Sbjct: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG
Sbjct: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360

Query: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ 420
           QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ
Sbjct: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ 420

Query: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGLPASELGN 480
           FIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGLPASELGN
Sbjct: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGLPASELGN 480

Query: 481 GGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGELKSD 540
           GGIGHVWSICEGSVEEEWRHSVAPIPAD+LEKILQKATDDELELTRDANVLPTSGELKSD
Sbjct: 481 GGIGHVWSICEGSVEEEWRHSVAPIPADRLEKILQKATDDELELTRDANVLPTSGELKSD 540

Query: 541 SNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAG 600
           SNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAG
Sbjct: 541 SNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAG 600

Query: 601 ALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLES 660
           ALFYSLM+FALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLES
Sbjct: 601 ALFYSLMKFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLES 660

Query: 661 AKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEE 720
           AKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEE
Sbjct: 661 AKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEE 720

Query: 721 EDDEEEE 727
           EDDEEEE
Sbjct: 721 EDDEEEE 727

BLAST of Csor.00g086820 vs. ExPASy TrEMBL
Match: A0A6J1KEW4 (eIF-2B GDP-GTP exchange factor subunit epsilon OS=Cucurbita maxima OX=3661 GN=LOC111493678 PE=3 SV=1)

HSP 1 Score: 1417 bits (3669), Expect = 0.0
Identity = 723/727 (99.45%), Postives = 724/727 (99.59%), Query Frame = 0

Query: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60
           MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL
Sbjct: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120
           SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER
Sbjct: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD
Sbjct: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180

Query: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240
           ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS
Sbjct: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG
Sbjct: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360

Query: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ 420
           QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELE GVILSFKVVVGDQ
Sbjct: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEAGVILSFKVVVGDQ 420

Query: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGLPASELGN 480
           FIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQG PASELGN
Sbjct: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGWPASELGN 480

Query: 481 GGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGELKSD 540
           GGIGHVWSICEGSVEEEWRHSVAPIPADKLEK LQKATDDELELTRDANVLPTSGELKSD
Sbjct: 481 GGIGHVWSICEGSVEEEWRHSVAPIPADKLEKTLQKATDDELELTRDANVLPTSGELKSD 540

Query: 541 SNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAG 600
           SNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAG
Sbjct: 541 SNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAADCAG 600

Query: 601 ALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLES 660
           ALFYSLM+FALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLES
Sbjct: 601 ALFYSLMKFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEMCLES 660

Query: 661 AKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEE 720
           AKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEE
Sbjct: 661 AKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRVASEE 720

Query: 721 EDDEEEE 727
           EDDEEEE
Sbjct: 721 EDDEEEE 727

BLAST of Csor.00g086820 vs. ExPASy TrEMBL
Match: A0A6J1G4Q9 (eIF-2B GDP-GTP exchange factor subunit epsilon OS=Cucurbita moschata OX=3662 GN=LOC111450765 PE=3 SV=1)

HSP 1 Score: 1416 bits (3665), Expect = 0.0
Identity = 724/731 (99.04%), Postives = 726/731 (99.32%), Query Frame = 0

Query: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60
           MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL
Sbjct: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120
           SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER
Sbjct: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD
Sbjct: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180

Query: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240
           ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS
Sbjct: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG
Sbjct: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360

Query: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ 420
           QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ
Sbjct: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ 420

Query: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSS----VADTTDKSNNELMSKSLEMQGLPAS 480
           FIVPSYSKVSLFQQPINQDSDDELEYADNSS     ADTTDKSNNELMSKSLEMQGLPAS
Sbjct: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSSGIVEFADTTDKSNNELMSKSLEMQGLPAS 480

Query: 481 ELGNGGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGE 540
           ELGNGGIGHVWSICEGSVEEEWRHSVAPIPAD+LEKILQKATDDELELTRDANVLPTSGE
Sbjct: 481 ELGNGGIGHVWSICEGSVEEEWRHSVAPIPADRLEKILQKATDDELELTRDANVLPTSGE 540

Query: 541 LKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAA 600
           LKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAA
Sbjct: 541 LKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAA 600

Query: 601 DCAGALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEM 660
           DCAGALFYSLM+FALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEM
Sbjct: 601 DCAGALFYSLMKFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEM 660

Query: 661 CLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRV 720
           CLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRV
Sbjct: 661 CLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRV 720

Query: 721 ASEEEDDEEEE 727
           ASEEEDDEEEE
Sbjct: 721 ASEEEDDEEEE 731

BLAST of Csor.00g086820 vs. ExPASy TrEMBL
Match: A0A6J1KGI1 (eIF-2B GDP-GTP exchange factor subunit epsilon OS=Cucurbita maxima OX=3661 GN=LOC111493678 PE=3 SV=1)

HSP 1 Score: 1410 bits (3649), Expect = 0.0
Identity = 722/731 (98.77%), Postives = 723/731 (98.91%), Query Frame = 0

Query: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60
           MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL
Sbjct: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120
           SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER
Sbjct: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD
Sbjct: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180

Query: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240
           ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS
Sbjct: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG
Sbjct: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360

Query: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ 420
           QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELE GVILSFKVVVGDQ
Sbjct: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEAGVILSFKVVVGDQ 420

Query: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSS----VADTTDKSNNELMSKSLEMQGLPAS 480
           FIVPSYSKVSLFQQPINQDSDDELEYADNSS     ADTTDKSNNELMSKSLEMQG PAS
Sbjct: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSSGIVEFADTTDKSNNELMSKSLEMQGWPAS 480

Query: 481 ELGNGGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGE 540
           ELGNGGIGHVWSICEGSVEEEWRHSVAPIPADKLEK LQKATDDELELTRDANVLPTSGE
Sbjct: 481 ELGNGGIGHVWSICEGSVEEEWRHSVAPIPADKLEKTLQKATDDELELTRDANVLPTSGE 540

Query: 541 LKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAA 600
           LKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAA
Sbjct: 541 LKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAA 600

Query: 601 DCAGALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEM 660
           DCAGALFYSLM+FALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEM
Sbjct: 601 DCAGALFYSLMKFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEM 660

Query: 661 CLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRV 720
           CLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRV
Sbjct: 661 CLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRV 720

Query: 721 ASEEEDDEEEE 727
           ASEEEDDEEEE
Sbjct: 721 ASEEEDDEEEE 731

BLAST of Csor.00g086820 vs. ExPASy TrEMBL
Match: A0A6J1CMU9 (eIF-2B GDP-GTP exchange factor subunit epsilon OS=Momordica charantia OX=3673 GN=LOC111013059 PE=3 SV=1)

HSP 1 Score: 1342 bits (3473), Expect = 0.0
Identity = 680/730 (93.15%), Postives = 707/730 (96.85%), Query Frame = 0

Query: 1   MGAQRKGASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYAL 60
           MGAQRKGASRVSED EELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINY L
Sbjct: 1   MGAQRKGASRVSEDPEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYER 120
           SWLESAGVEEVFVFCCA+SKQVIDYLENSEWVSLPDFAVTTIESHNS+SAGDALRLIYER
Sbjct: 61  SWLESAGVEEVFVFCCAHSKQVIDYLENSEWVSLPDFAVTTIESHNSVSAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLITGDTISNM LT+ALQ+HK+RKKKDSNAVMTMVVKRSKPSPITHQSRLGTD
Sbjct: 121 NVIHGDFVLITGDTISNMSLTRALQQHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180

Query: 181 ELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLS 240
           ELFMAIDHN+KQLLYYED+AD SKG+IHLDKSLLMDN SISLHND+QDCYIDICSPEVLS
Sbjct: 181 ELFMAIDHNTKQLLYYEDKADHSKGVIHLDKSLLMDNSSISLHNDMQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGN AYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG
Sbjct: 301 YPLVPDVKFFGNFAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360

Query: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSFKVVVGDQ 420
           QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAEL+PGV+LSFKVV+GDQ
Sbjct: 361 QGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELQPGVVLSFKVVIGDQ 420

Query: 421 FIVPSYSKVSLFQQPINQDSDDELEYADNSS----VADTTDKSNNELMSKSLEMQGLPAS 480
           F+VPSYSKVSLFQQP NQDSD+ELEYADNSS       TTDKSN+ELMSKSLEMQG P S
Sbjct: 421 FVVPSYSKVSLFQQPTNQDSDEELEYADNSSGIVEFTGTTDKSNSELMSKSLEMQGWPTS 480

Query: 481 ELGNGGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPTSGE 540
           ELGNGG+G+VWSICEGSVEEEWRHSVAPIPADKLEK +Q ATDDELELT+DANVLP SGE
Sbjct: 481 ELGNGGMGYVWSICEGSVEEEWRHSVAPIPADKLEKAMQ-ATDDELELTQDANVLPPSGE 540

Query: 541 LKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNKVAA 600
           LKSDS VSDGDDNEDSRDDSIHFEKEVEATFLR VHENIKVDNVILEVNSLRLSYNKVAA
Sbjct: 541 LKSDSYVSDGDDNEDSRDDSIHFEKEVEATFLRAVHENIKVDNVILEVNSLRLSYNKVAA 600

Query: 601 DCAGALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKFEEM 660
           DCAGA+FYSLM+FALDSPH SSSELLQN+TN+ITKWKKLLKYYL+DMDEEIEVILKFEE+
Sbjct: 601 DCAGAIFYSLMKFALDSPHSSSSELLQNSTNVITKWKKLLKYYLSDMDEEIEVILKFEEI 660

Query: 661 CLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIEWLRV 720
           CLESAKEFTPHFTKILCHLYDQEI+QEDAILRWDDEKKEADDSDK FVKQAEKFIEWLRV
Sbjct: 661 CLESAKEFTPHFTKILCHLYDQEIVQEDAILRWDDEKKEADDSDKFFVKQAEKFIEWLRV 720

Query: 721 ASEEEDDEEE 726
           ASEEED+EEE
Sbjct: 721 ASEEEDEEEE 729

BLAST of Csor.00g086820 vs. TAIR 10
Match: AT2G34970.1 (Trimeric LpxA-like enzyme )

HSP 1 Score: 929.9 bits (2402), Expect = 1.3e-270
Identity = 475/735 (64.63%), Postives = 587/735 (79.86%), Query Frame = 0

Query: 1   MGAQRKG--ASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINY 60
           MGAQ+KG  A+RVSED+E  +R  LQA+LLADSF TKFRP+TLERPKVLLP+VNVPMI+Y
Sbjct: 1   MGAQKKGGAAARVSEDAEVQSRHRLQAILLADSFATKFRPVTLERPKVLLPIVNVPMIDY 60

Query: 61  ALSWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIY 120
            L+WLESAG+EEVFVFCCA+S QVI+YLE SEW S P+  V TIESH SISAGDALR +Y
Sbjct: 61  TLAWLESAGIEEVFVFCCAHSMQVIEYLEKSEWYSHPNLLVRTIESHKSISAGDALRYMY 120

Query: 121 ERNV----IHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQ 180
           E+      I GDFVL++GDT+SNMPL   +QEH++RKKKD  A+MTMV+K+SK SP+THQ
Sbjct: 121 EQQTETSQIQGDFVLVSGDTVSNMPLADLIQEHRERKKKDEKAIMTMVIKQSKSSPLTHQ 180

Query: 181 SRLGTDELFMAIDHNSKQLLYY-EDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDI 240
           SRLGTD+LF+A+D  +KQLL+Y ED+ D   G + L+KSLL  NPS+ + ND+QDCYIDI
Sbjct: 181 SRLGTDQLFIAVDPLTKQLLHYEEDKIDHPSGSVCLEKSLLDTNPSVLVCNDMQDCYIDI 240

Query: 241 CSPEVLSLFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSK 300
           CSPEVLSLF DNFDYQHLRRHFVKG+L+DDIMGYKI+THEI SSYA RIDN+RSYDTVSK
Sbjct: 241 CSPEVLSLFEDNFDYQHLRRHFVKGVLVDDIMGYKIFTHEIHSSYAGRIDNFRSYDTVSK 300

Query: 301 DIIQRWTYPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTK 360
           DIIQRWTYP VPD+ F GN   KL RQG+Y+AS+++Q+RSA VG  TVIG  +KIG   K
Sbjct: 301 DIIQRWTYPYVPDINFSGNRPLKLGRQGIYKASDVVQSRSADVGASTVIGYGTKIGHGDK 360

Query: 361 ITNSIIGQGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSF 420
           I NS+IG GCSIGSNV IEGSYIW+NVTIEDGC++ +AIVCDGV I++GA L+PGV+LSF
Sbjct: 361 IMNSVIGNGCSIGSNVVIEGSYIWNNVTIEDGCEIRNAIVCDGVKIRAGAVLQPGVVLSF 420

Query: 421 KVVVGDQFIVPSYSKVSLFQQPINQDSDDELEYADNSS-VADTTDKSNNELMSKSLEMQG 480
            VVVG  F+VP+YSKVSL QQP  +DSD+ELEYAD+SS  AD     N ++ SK      
Sbjct: 421 NVVVGRDFVVPAYSKVSLLQQPTTEDSDEELEYADSSSGTADHLSGLNLQMESK------ 480

Query: 481 LPASELGNGGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLP 540
             ASELG  G G++W +CEG+ +EEW+HSVAPIP DKL +I Q   DD+   T D +V+P
Sbjct: 481 --ASELGPDGAGYIWEVCEGAHDEEWKHSVAPIPKDKLSEITQAIDDDD---TDDESVVP 540

Query: 541 TSGELKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYN 600
           TSGELKSD++  + D N D  DD  +FEKEVE T LR V ENIKVD V +E+N LRLS+N
Sbjct: 541 TSGELKSDADSINTDVN-DPNDDYYYFEKEVEGTVLRAVEENIKVDLVTMEINGLRLSFN 600

Query: 601 KVAADCAGALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILK 660
             +ADCAGA F+S+++ ALD+PH S SEL +N  +IITKWK LL +Y   +DE+IEVI+K
Sbjct: 601 MESADCAGATFFSMIKLALDTPHNSGSELYKNAASIITKWKDLLGFYAKKIDEQIEVIMK 660

Query: 661 FEEMCLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAEKFIE 720
           FEEMC ES KE  P FT+IL  LYD++++QEDAILRW++EK  AD++DK+++KQ + FI+
Sbjct: 661 FEEMCQESHKELGPLFTQILHLLYDKDVLQEDAILRWEEEKAGADEADKVYLKQCDTFIQ 720

Query: 721 WLRVASEEEDDEEEE 728
           WL+ ASEEED+++E+
Sbjct: 721 WLKEASEEEDEDDED 723

BLAST of Csor.00g086820 vs. TAIR 10
Match: AT4G18300.1 (Trimeric LpxA-like enzyme )

HSP 1 Score: 816.2 bits (2107), Expect = 2.1e-236
Identity = 437/736 (59.38%), Postives = 554/736 (75.27%), Query Frame = 0

Query: 1   MGAQRK-GASRVSEDSEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYA 60
           MGAQ+K  A++V EDSE+L+R  LQA+LLADSFTT+FR ITLERPKVLLP+VN+PMI+Y 
Sbjct: 1   MGAQKKPAAAKVREDSEDLSRQRLQAILLADSFTTQFRSITLERPKVLLPIVNIPMIDYT 60

Query: 61  LSWLESAGVEEVFVFCCANSKQVIDYLENSEWVSL--PDFAVTTIESHNSISAGDALRLI 120
           L+WLESAG+EEVFVFCCA+S Q+IDYL+ SEW +   P+  V TI SHNS S GDALR I
Sbjct: 61  LAWLESAGIEEVFVFCCAHSSQIIDYLKKSEWYTRPNPNLLVRTIVSHNSTSVGDALRYI 120

Query: 121 YERNV----IHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITH 180
           YE+      I GDFVL++GDT+SNMPL   +Q+H+DRKKKD  A+MTMV K+        
Sbjct: 121 YEQQTETSQIQGDFVLVSGDTVSNMPLADLIQQHRDRKKKDEKAIMTMVFKK-------- 180

Query: 181 QSRL--GTDELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYI 240
           QSRL  G+D+LF+A+D  +KQLL+YE+  +   G   LDKSLL    S  L +D+QDCYI
Sbjct: 181 QSRLGIGSDQLFVAVDPLTKQLLHYEE-CNSRGGDFCLDKSLL---DSTVLCSDMQDCYI 240

Query: 241 DICSPEVLSLFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSS-YAARIDNYRSYDT 300
           DICSPEVLSLF DNFDYQH+RRHFV GLL+DDIMGYKI+THEI SS YAARIDN RSYDT
Sbjct: 241 DICSPEVLSLFEDNFDYQHMRRHFVNGLLVDDIMGYKIFTHEIQSSCYAARIDNLRSYDT 300

Query: 301 VSKDIIQRWTYPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGD 360
           VSKDIIQRWTYP VP++ F GN   KL R+G+YRAS+ +Q+ +A VG  TVIG  +KIG+
Sbjct: 301 VSKDIIQRWTYPYVPNIDFSGNRPVKLGRRGIYRASDAVQSHTADVGASTVIGYGTKIGN 360

Query: 361 NTKITNSIIGQGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVI 420
             KI NS+IG GC IGSNV I+GSYIW+NVTIEDGC++ +AIVCDGV +++GA L+PGV+
Sbjct: 361 GGKIYNSVIGNGCCIGSNVVIQGSYIWNNVTIEDGCEIRNAIVCDGVKVRAGAVLQPGVV 420

Query: 421 LSFKVVVGDQFIVPSYSKVSLFQQPINQDSDDELEYADNSS-VADTTDKSNN-ELMSKSL 480
           LSF VVVG  F+VP+YSKVSL QQP  +DSD+ELEYAD+SS  AD +   NN ++ SK  
Sbjct: 421 LSFSVVVGRDFVVPAYSKVSLLQQPTTEDSDEELEYADSSSGTADHSSGLNNLQIESK-- 480

Query: 481 EMQGLPASELGNGGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDA 540
                 ASELG  G G++W IC+ + +EEW+HSV PIP DKL  I     DD++E   D 
Sbjct: 481 ------ASELGPDGAGYIWEICDSAHDEEWKHSVVPIPMDKLADITGAMDDDDIE---DD 540

Query: 541 NVLPTSGELKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLR 600
            V+P SGELKSD+++ + D N+   D    FE+EVE  FL  V ++ K D VI  +NS R
Sbjct: 541 IVVPPSGELKSDTDIINTDANDSYGD----FEREVEEEFLSAVEKDNKADIVIPLINSHR 600

Query: 601 LSYNKVAADCAGALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIE 660
           L+YN  +ADCAGA+FYS+M+ A+ SPH S++EL +N   +ITKWK +L +YL   D +IE
Sbjct: 601 LAYNMDSADCAGAVFYSMMRLAVKSPHNSATELYRNAMGVITKWKGVLGFYLKQTDAQIE 660

Query: 661 VILKFEEMCLESAKEFTPHFTKILCHLYDQEIIQEDAILRWDDEKKEADDSDKLFVKQAE 720
           VI+KFEEMC ES +E +P F K+L  LYD++++QEDAILRW +EK  ADD DK+++ + E
Sbjct: 661 VIMKFEEMCQES-EELSPLFAKLLPFLYDKDVVQEDAILRWGEEKAGADDCDKVYLNKCE 708

Query: 721 KFIEWLRVASEEEDDE 725
            FI+WL+ ASEEE+++
Sbjct: 721 SFIKWLKEASEEEEED 708

BLAST of Csor.00g086820 vs. TAIR 10
Match: AT3G02270.1 (Trimeric LpxA-like enzyme )

HSP 1 Score: 735.7 bits (1898), Expect = 3.5e-212
Identity = 401/731 (54.86%), Postives = 515/731 (70.45%), Query Frame = 0

Query: 1   MGAQRKGASRVSEDSEE--LARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINY 60
           M +++K A+++SEDSEE    R  LQA+LLADSF TK  P+TLERP VLLPLVN+PMI+Y
Sbjct: 1   MASRKKRAAKISEDSEEEQSRRQRLQAILLADSFATKLLPLTLERPNVLLPLVNIPMIDY 60

Query: 61  ALSWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVTTIES-HNSISAGDALRLI 120
            L+WLESAG+EEVFVFC   S QVIDYL NS+W S  DF V TIES  NS SAGDALR I
Sbjct: 61  TLAWLESAGIEEVFVFC---SMQVIDYLNNSDWYSHKDFTVKTIESPQNSTSAGDALRYI 120

Query: 121 YERNV----IHGDFVLITGDTISNMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITH 180
           YE+ +    I GDFVL+ G  +SNMPLTQ +QEH+DRKKKD  A+MTMV++         
Sbjct: 121 YEQQIETSQIQGDFVLVNGCIVSNMPLTQLIQEHRDRKKKDEKAIMTMVIR--------- 180

Query: 181 QSRLGTDELFMAIDHNSKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDI 240
           QS +   +LF+A++  +KQLLYY++        I  DKSLL  NPS+ L +D+QDCYIDI
Sbjct: 181 QSLITDHQLFIAVNPLTKQLLYYDE------DNICFDKSLLDRNPSVLLCSDMQDCYIDI 240

Query: 241 CSPEVLSLFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSK 300
           CS EVLSLF DNFDYQH+R  FV+G+L DDI+GYKI+THEI S YA+RI+N+RSYD VSK
Sbjct: 241 CSLEVLSLFVDNFDYQHMRCDFVEGVLADDIIGYKIFTHEISSCYASRIENFRSYDMVSK 300

Query: 301 DIIQRWTYPLVPDVKFFGNSAYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTK 360
           DIIQR T+P VPD+KF GN   KLERQG+Y+AS+  Q  SA VG   VIG  + IG  TK
Sbjct: 301 DIIQRRTFPYVPDMKFSGNRTLKLERQGIYKASDATQLPSAHVGASYVIGHATNIGSGTK 360

Query: 361 ITNSIIGQGCSIGSNVTIEGSYIWDNVTIEDGCKLSHAIVCDGVIIKSGAELEPGVILSF 420
           I NS+IG GCSIGSNV I+GSYIW+NVT+EDGC++ +AIVCD V + +GA ++PGV+LSF
Sbjct: 361 ILNSVIGNGCSIGSNVVIQGSYIWNNVTVEDGCEIRNAIVCDEVKVCAGAIVKPGVVLSF 420

Query: 421 KVVVGDQFIVPSYSKVSLFQQPINQDSDDELEYADNSSVADTTDKSNNELMSKSLEMQGL 480
           KVVVG  F+VP+YS+VSL +QP+ +DSD+E                 N L    L+M+  
Sbjct: 421 KVVVGRDFVVPAYSQVSLLRQPMEEDSDEE-----------------NLLSGVDLQME-- 480

Query: 481 PASELGNGGIGHVWSICEGSVEEEWRHSVAPIPADKLEKILQKATDDELELTRDANVLPT 540
             S+LG  G G++W     + E+EW+HSV PIP DKL +I++   DD+   T D +V+ T
Sbjct: 481 --SKLGLDGAGYIW---RQACEDEWKHSVPPIPKDKLAEIIKAIDDDD---TDDESVVTT 540

Query: 541 SGELKSDSNVSDGDDNEDSRDDSIHFEKEVEATFLRGVHENIKVDNVILEVNSLRLSYNK 600
           S           GD N    +D   FE+EV+ TFLR V ENI  D  +LE+NSLRLSYN 
Sbjct: 541 S-----------GDANTSINNDLFDFEREVDGTFLRAVEENIVADLAVLEINSLRLSYNM 600

Query: 601 VAADCAGALFYSLMQFALDSPHGSSSELLQNTTNIITKWKKLLKYYLTDMDEEIEVILKF 660
            +A CAGA+FYS+M+ A+ +PH S ++L +N ++IIT+WK LL +Y+   DE+IEVI + 
Sbjct: 601 ESAHCAGAIFYSMMKLAVSTPHSSINDLYRNASSIITRWKGLLGFYVKKSDEQIEVISRL 660

Query: 661 EEMCLESAKEFTPHFTKILCHLYDQE--IIQEDAILRWDDEKKEADDSDKLFVKQAEKFI 720
           EEMC ESA E    F  IL ++Y++E  ++QE AILRW DEK  AD+SDK+++KQ E FI
Sbjct: 661 EEMCEESAHELGTLFAHILRYMYEEENDLLQEVAILRWSDEKAGADESDKVYLKQCEPFI 675

Query: 721 EWLRVASEEED 723
            WL+  S++ED
Sbjct: 721 TWLKETSDDED 675

BLAST of Csor.00g086820 vs. TAIR 10
Match: AT5G19485.1 (transferases;nucleotidyltransferases )

HSP 1 Score: 101.7 bits (252), Expect = 2.6e-21
Identity = 101/457 (22.10%), Postives = 186/457 (40.70%), Query Frame = 0

Query: 21  VSLQAVLLADSFTTKFRP-ITLERPKVLLPLVNVPMINYALSWLESAGVEEVFVFCCANS 80
           +  Q V+LA  F++   P +  E PK LLP+ N P+++Y L  LES+ ++++ V      
Sbjct: 1   MDFQVVILAGGFSSYLVPLVAKEVPKALLPVANRPVLSYVLDLLESSNLKDLIVVVEGED 60

Query: 81  KQVIDYLENSEWVS---LPDFAVTTIESHNSISAGDALRLIYERNVIHGDFVLITGDTIS 140
                 L+   W+S   +    V       ++    ALR I   ++   D ++++GD +S
Sbjct: 61  AA----LKVGGWISSACVDRLHVEVAAVAENVGTAGALRAI-AHHLTAKDILIVSGDIVS 120

Query: 141 NMPLTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTDEL-------FMAIDHN 200
           ++P       H    ++   AV  M+  +    P       G D+         + +D +
Sbjct: 121 DIPPGAVAATH----RRHDAAVTVMLCAQPVSGPSESGGSGGKDKTKKPACDDIIGLD-S 180

Query: 201 SKQLLYYEDRADDSKGIIHLDKSLLMDNPSISLHNDLQDCYIDICSPEVLSLFTDNFD-- 260
            KQ L Y  +  + K    + KS+L     + + +DL D +I      VL    D     
Sbjct: 181 LKQFLLYIAKGTEIKKDTRVKKSILCAAGKMEIRSDLMDSHIYAFKRAVLQEVLDQKPAF 240

Query: 261 ---YQHLRRHFVKGLLLDDIMG------------------------------------YK 320
               Q +  + V+  L  D+                                      ++
Sbjct: 241 RSLKQDVLPYLVRTQLRSDVFSDQSNVEENGNGNGKNNMQNNEVVLSQILSNASMPSFHQ 300

Query: 321 IY--------THEIC------SSYAARIDNYRSYDTVSKDIIQRWTYPLVPDVKFFGNSA 380
           +Y        TH+ C      S Y  R+++ +++  V++D+I         +       +
Sbjct: 301 VYESGLGTRKTHKCCVYIADESKYFVRLNSIQAFMDVNRDVI--------GESNHLSGYS 360

Query: 381 YKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGQGCSIGSNVTIEGS 412
           +      ++ ++E+       VGP  ++G+ S++GD   +  S+IG+ C IGSNV I  S
Sbjct: 361 FSAHHNIVHPSAEL--GSKTTVGPHCMLGEGSQVGDKCSVKRSVIGRHCRIGSNVKIVNS 420

BLAST of Csor.00g086820 vs. TAIR 10
Match: AT2G39770.1 (Glucose-1-phosphate adenylyltransferase family protein )

HSP 1 Score: 61.2 bits (147), Expect = 3.9e-09
Identity = 50/195 (25.64%), Postives = 97/195 (49.74%), Query Frame = 0

Query: 23  LQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYALSWLESAGVEEVFVFCCANSKQV 82
           ++A++L   F T+ RP+TL  PK L+   N PMI + +  L++ GV+EV +      + +
Sbjct: 1   MKALILVGGFGTRLRPLTLSFPKPLVDFANKPMILHQIEALKAVGVDEVVLAINYQPEVM 60

Query: 83  IDYLENSEWVSLPDFAVTTIESHNSISAGDALRLIYERNVIHGD---FVLITGDTISNMP 142
           +++L++ E  +  +  +T  +    +     L L  ++ ++ G    F ++  D IS  P
Sbjct: 61  LNFLKDFE--TKLEIKITCSQETEPLGTAGPLALARDK-LLDGSGEPFFVLNSDVISEYP 120

Query: 143 LTQALQEHKDRKKKDSNAVMTMVVKRSKPSPITHQSRLGTDELFMAIDHNSKQLLYYEDR 202
           L + L+ HK     +++ ++T V + SK   +  +   G  E F+      K  LY  ++
Sbjct: 121 LKEMLEFHKSH-GGEASIMVTKVDEPSKYGVVVMEESTGRVEKFV-----EKPKLYVGNK 180

Query: 203 ADDSKGIIHLDKSLL 215
              + GI  L+ S+L
Sbjct: 181 I--NAGIYLLNPSVL 184

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54RF38.4e-12635.43Translation initiation factor eIF-2B subunit epsilon OS=Dictyostelium discoideum... [more]
P562878.5e-11032.35Probable translation initiation factor eIF-2B subunit epsilon OS=Schizosaccharom... [more]
P871631.2e-10833.60Translation initiation factor eIF-2B subunit epsilon OS=Candida albicans (strain... [more]
Q643504.5e-10332.74Translation initiation factor eIF-2B subunit epsilon OS=Rattus norvegicus OX=101... [more]
Q131447.7e-10332.83Translation initiation factor eIF-2B subunit epsilon OS=Homo sapiens OX=9606 GN=... [more]
Match NameE-valueIdentityDescription
KAG6599283.10.0100.00hypothetical protein SDJN03_09061, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022946813.10.099.72translation initiation factor eIF-2B subunit epsilon-like isoform X2 [Cucurbita ... [more]
XP_022999250.10.099.45translation initiation factor eIF-2B subunit epsilon-like isoform X2 [Cucurbita ... [more]
XP_022946812.10.099.04translation initiation factor eIF-2B subunit epsilon-like isoform X1 [Cucurbita ... [more]
XP_022999249.10.098.77translation initiation factor eIF-2B subunit epsilon-like isoform X1 [Cucurbita ... [more]
Match NameE-valueIdentityDescription
A0A6J1G4V40.099.72eIF-2B GDP-GTP exchange factor subunit epsilon OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KEW40.099.45eIF-2B GDP-GTP exchange factor subunit epsilon OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1G4Q90.099.04eIF-2B GDP-GTP exchange factor subunit epsilon OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KGI10.098.77eIF-2B GDP-GTP exchange factor subunit epsilon OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1CMU90.093.15eIF-2B GDP-GTP exchange factor subunit epsilon OS=Momordica charantia OX=3673 GN... [more]
Match NameE-valueIdentityDescription
AT2G34970.11.3e-27064.63Trimeric LpxA-like enzyme [more]
AT4G18300.12.1e-23659.38Trimeric LpxA-like enzyme [more]
AT3G02270.13.5e-21254.86Trimeric LpxA-like enzyme [more]
AT5G19485.12.6e-2122.10transferases;nucleotidyltransferases [more]
AT2G39770.13.9e-0925.64Glucose-1-phosphate adenylyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 507..527
NoneNo IPR availableGENE3D2.160.10.10Hexapeptide repeat proteinscoord: 319..425
e-value: 1.2E-17
score: 65.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 541..556
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 524..556
NoneNo IPR availablePANTHERPTHR45887TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILONcoord: 10..725
NoneNo IPR availableCDDcd05787LbH_eIF2B_epsiloncoord: 341..419
e-value: 4.74774E-39
score: 136.939
IPR003307W2 domainSMARTSM00515542_3coord: 633..720
e-value: 4.8E-25
score: 99.2
IPR003307W2 domainPFAMPF02020W2coord: 646..724
e-value: 5.3E-17
score: 61.8
IPR003307W2 domainPROSITEPS51363W2coord: 553..724
score: 27.562117
IPR029044Nucleotide-diphospho-sugar transferasesGENE3D3.90.550.10Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain Acoord: 9..303
e-value: 1.5E-37
score: 131.5
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILY53448Nucleotide-diphospho-sugar transferasescoord: 22..369
IPR005835Nucleotidyl transferase domainPFAMPF00483NTP_transferasecoord: 25..168
e-value: 1.0E-9
score: 38.3
IPR016021MIF4G-like domain superfamilyGENE3D1.25.40.180coord: 546..725
e-value: 1.2E-50
score: 173.8
IPR001451Hexapeptide repeatPFAMPF00132Hexapepcoord: 340..373
e-value: 6.3E-7
score: 28.9
IPR044123Translation initiation factor eIF-2B subunit epsilon, W2 domainCDDcd11558W2_eIF2B_epsiloncoord: 554..718
e-value: 4.88663E-46
score: 159.732
IPR035543Translation initiation factor eIF-2B subunit epsilon, N-terminalCDDcd04197eIF-2B_epsilon_Ncoord: 23..239
e-value: 6.35046E-95
score: 291.819
IPR011004Trimeric LpxA-like superfamilySUPERFAMILY51161Trimeric LpxA-like enzymescoord: 267..429
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 559..715

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g086820.m01Csor.00g086820.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0006413 translational initiation
biological_process GO:0009058 biosynthetic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0005851 eukaryotic translation initiation factor 2B complex
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity
molecular_function GO:0016779 nucleotidyltransferase activity
molecular_function GO:0003743 translation initiation factor activity
molecular_function GO:0031369 translation initiation factor binding
molecular_function GO:0005515 protein binding