Csor.00g085730 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g085730
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description(1->3)-beta-glucan endohydrolase
LocationCsor_Chr05: 10352828 .. 10355264 (-)
RNA-Seq ExpressionCsor.00g085730
SyntenyCsor.00g085730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAGAAAATGCTTCTGGCTCTGTGTTCTATCGCTTCCTGGTTTCTGGATCGTCTTCTCTGCCATTGGTACTGTGTAACTTTTTCTCTTTCTGTTTTCTAGTTCTTCATGTTTAGTAATCAGATGGCTCTGAGTTTTTGGTTTTGGATTTTGGGTCCAAAGATTGCTTCCATTTGACTTATATTCAGAAGTTTTCATGGATTTTCCTCTTCTTTTTGCTTGATTTAGTAAGGTCAGCTCATGATTCTGGGCAGTGACAACCAGAATTCTTTATCTTATGCTTCTGGCTTGCAAGTTTTGAGACTGTTGAGGACTTTGTGCCTGCATCATTTCTTTTATTTGATGGAGTTTTCTTCTGGGTTAGTTTTGATTGTTTCTTTAAGTTCTATTTCGTGCTTCTTTCAGTCATTCTTGAAGATTTTTGTTGAGAACTGAGAGTAAATTTTGATGTATGTTTTTAAAACTCTTGTTGTTTCTTGTTAAGTCGTCCACTTGGGGAAGTTGTAGTATGTGAGATTCCAGGCTGGTTGGAGAGGAGAATGAAGCATTTCTTATAAAGGTGTGGAAACCTCTTCCCAGTAGACTCATTTTAAAACCTTGAGAGGAAGCCCGGAAGGAAAAGCCCAAAAAGGACAATATTTGCTAGCGGTGGGCTTGGGCTGTTACAAATGGTATCAGAGCTAGACATCGGGTGGTGTGCCAACGAGGACGCTGGATCCCCAAGGGGGGTGGATTGTGAGATCCCACATTGGTTGGAGAGGAGAACGACACATTCCTTATAAGGATGTGGAAACCTCTCTCTAGTAGACACGTCTTCCCAGAAAGGAAAGCTCAAAGATGACAATATCTGCTAATAGTGGACTTGGGCTTTTACAAATGGTATCAGAGCCAGACATTGGGCGGTGTGCCAGTGAGGACGTTGGACCTCTAAAGAGGGTGGATTGTGAGATCCCACATTGATTGGAGATGAGCACAAAGCATTCCTTATAAGGGTATGGAAACCTCTCCCTAGTAGACGCGTTTTAAAACTTTGAGGGGAAGTTCGGAAGGAAAAGCCTAAAGAAGACAATATCTGTTAGCGGTGGGCTTGGGTTTTTATGTAGTAAAACATCTCCTTGTCTTTCTCCAGCCTCCTCGACAATGACAGTACAAGCATTTACTGGAACTTATGGAATAAATTATGGAAGGATTGCTGATAACATTCCTTCACCTGATGAAGTTGTTTCACTTCTCAAAGCAGCAAAAATAAAGAACGTTAGGATTTATGATGCTGATCACAATGTCCTGAAGGCGTTTAGTGGGACAGGGCTTGAATTAGAGGTTGGTCTCCCAAATGGGTTCTTGAAAGAAACGAGTGCGAGCGAGGAACACGCGATGAACTGGGTCAAAGAAAATGTACAGGCATTCCTTCCTGAGACTCACATTTGTGGGATTGCCATTGGTAATGAAGTTCTCGGAGGAGGTGACCTTGAGCTGTGGGGAGCACTCTTGGGTGCTGCAAAGAATATCTACAAGGCAGTAAAGAGCCTTAACTTAGATGGTGTTGTTCAGATCACAACCGCGCATTCTCAGGCTGTTTTTTCGAACTCGTTTCCGCCTTCTTCGTGTATATTTAGAGAAAATGTAGTTCAGTACATGAGGCCTTTGCTGGAGTTTCTTACAGAGATTGGCTCACCTTTCTGTTTAAACGCTTACCCCTTCCTAGCCTATATGAGTGACCCTGAGAATATTGATATCAACTATGCACTTTTTGAATCAACTAAGGGTATTATTGATCCCAAAACTGGTCTACATTATGACAACATGCTTGATGCTCAGATCGACGCTGCATATGCAGCGTTAGCAGACGCTGGATATCGGAAAATGGAAGTCATAGTTACCGAGACTGGTTGGGCTTCCCGTGGGGACGAAAATGAGGCTGCAGCAACGGTGAGTAATGCTAAGATATTTAATTACAACCTACGGAAGAGGCTTGCTAAGAAGAAAGGAACTCCCTACAGGCCAAGGAATGTATTGAAGGCCTACGTTTTTGCAACGTTTAACGAAAATTTGAAACAGGGGCCGACGTCTGAGAGGAATTTTGGACTCTTCAAAGCTGATGGGAGCATATCATATGATGTTGGATTTCATGGACTTAAATCTTCTTCTTCAGACTCATCTTATTTATCTCTCAAGGTATGTAATTCATCACACCATATGAAACATGTTATGTTTGTTGAAACTATGTTAAGATAATATCGTTACCTAGGGAAGTCGAAAGTTATTGTATTCTAATCCTACCTTGATCTTAGTTTGTACTAAACACCGTCTTTAACGATATTCATACACGTCTTACTTGCTTATGTGAACAGAGTTTCCGAGCTCGAGGACTTTCAGCTAACCACATTTTCTCATTAACATTCTCGGCCGTGACGCTACTTCTATTGTTGAGATGA

mRNA sequence

ATGGCCAGAAAATGCTTCTGGCTCTGTGTTCTATCGCTTCCTGGTTTCTGGATCGTCTTCTCTGCCATTGCCTCCTCGACAATGACAGTACAAGCATTTACTGGAACTTATGGAATAAATTATGGAAGGATTGCTGATAACATTCCTTCACCTGATGAAGTTGTTTCACTTCTCAAAGCAGCAAAAATAAAGAACGTTAGGATTTATGATGCTGATCACAATGTCCTGAAGGCGTTTAGTGGGACAGGGCTTGAATTAGAGGTTGGTCTCCCAAATGGGTTCTTGAAAGAAACGAGTGCGAGCGAGGAACACGCGATGAACTGGGTCAAAGAAAATGTACAGGCATTCCTTCCTGAGACTCACATTTGTGGGATTGCCATTGGTAATGAAGTTCTCGGAGGAGGTGACCTTGAGCTGTGGGGAGCACTCTTGGGTGCTGCAAAGAATATCTACAAGGCAGTAAAGAGCCTTAACTTAGATGGTGTTGTTCAGATCACAACCGCGCATTCTCAGGCTGTTTTTTCGAACTCGTTTCCGCCTTCTTCGTGTATATTTAGAGAAAATGTAGTTCAGTACATGAGGCCTTTGCTGGAGTTTCTTACAGAGATTGGCTCACCTTTCTGTTTAAACGCTTACCCCTTCCTAGCCTATATGAGTGACCCTGAGAATATTGATATCAACTATGCACTTTTTGAATCAACTAAGGGTATTATTGATCCCAAAACTGGTCTACATTATGACAACATGCTTGATGCTCAGATCGACGCTGCATATGCAGCGTTAGCAGACGCTGGATATCGGAAAATGGAAGTCATAGTTACCGAGACTGGTTGGGCTTCCCGTGGGGACGAAAATGAGGCTGCAGCAACGGGGCCGACGTCTGAGAGGAATTTTGGACTCTTCAAAGCTGATGGGAGCATATCATATGATGTTGGATTTCATGGACTTAAATCTTCTTCTTCAGACTCATCTTATTTATCTCTCAAGAGTTTCCGAGCTCGAGGACTTTCAGCTAACCACATTTTCTCATTAACATTCTCGGCCGTGACGCTACTTCTATTGTTGAGATGA

Coding sequence (CDS)

ATGGCCAGAAAATGCTTCTGGCTCTGTGTTCTATCGCTTCCTGGTTTCTGGATCGTCTTCTCTGCCATTGCCTCCTCGACAATGACAGTACAAGCATTTACTGGAACTTATGGAATAAATTATGGAAGGATTGCTGATAACATTCCTTCACCTGATGAAGTTGTTTCACTTCTCAAAGCAGCAAAAATAAAGAACGTTAGGATTTATGATGCTGATCACAATGTCCTGAAGGCGTTTAGTGGGACAGGGCTTGAATTAGAGGTTGGTCTCCCAAATGGGTTCTTGAAAGAAACGAGTGCGAGCGAGGAACACGCGATGAACTGGGTCAAAGAAAATGTACAGGCATTCCTTCCTGAGACTCACATTTGTGGGATTGCCATTGGTAATGAAGTTCTCGGAGGAGGTGACCTTGAGCTGTGGGGAGCACTCTTGGGTGCTGCAAAGAATATCTACAAGGCAGTAAAGAGCCTTAACTTAGATGGTGTTGTTCAGATCACAACCGCGCATTCTCAGGCTGTTTTTTCGAACTCGTTTCCGCCTTCTTCGTGTATATTTAGAGAAAATGTAGTTCAGTACATGAGGCCTTTGCTGGAGTTTCTTACAGAGATTGGCTCACCTTTCTGTTTAAACGCTTACCCCTTCCTAGCCTATATGAGTGACCCTGAGAATATTGATATCAACTATGCACTTTTTGAATCAACTAAGGGTATTATTGATCCCAAAACTGGTCTACATTATGACAACATGCTTGATGCTCAGATCGACGCTGCATATGCAGCGTTAGCAGACGCTGGATATCGGAAAATGGAAGTCATAGTTACCGAGACTGGTTGGGCTTCCCGTGGGGACGAAAATGAGGCTGCAGCAACGGGGCCGACGTCTGAGAGGAATTTTGGACTCTTCAAAGCTGATGGGAGCATATCATATGATGTTGGATTTCATGGACTTAAATCTTCTTCTTCAGACTCATCTTATTTATCTCTCAAGAGTTTCCGAGCTCGAGGACTTTCAGCTAACCACATTTTCTCATTAACATTCTCGGCCGTGACGCTACTTCTATTGTTGAGATGA

Protein sequence

MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPETHICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDPKTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAATGPTSERNFGLFKADGSISYDVGFHGLKSSSSDSSYLSLKSFRARGLSANHIFSLTFSAVTLLLLLR
Homology
BLAST of Csor.00g085730 vs. ExPASy Swiss-Prot
Match: Q8L868 (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860 PE=2 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.4e-68
Identity = 132/336 (39.29%), Postives = 198/336 (58.93%), Query Frame = 0

Query: 23  IASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHNVLKAFSGT 82
           I+ + + +   T + G+NYG+I DN+PSP +V+ L+K+     V++YDA+  +LKAFS T
Sbjct: 15  ISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILKAFSNT 74

Query: 83  GLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPETHICGIAIGNEVLGGGDLELWGA 142
           G+E  +GL N +L +       A+ W+K+NV  FLP T+I  I IGNE+L   D  L   
Sbjct: 75  GIEFIIGLGNEYLSKMK-DPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSSLTTN 134

Query: 143 LLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMRPLLEFLTE 202
           LL A + ++ A+ +  L   + +TTAHS ++  +SFPPS+  F+ +++  + P+LEF  +
Sbjct: 135 LLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILEFHRK 194

Query: 203 IGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDPKTGLHYDNMLDAQIDAAYAALA 262
             SPF +NAYPF AY  +P+ + +++ LF+  +GI+DP TG HYDNML AQIDA Y+ALA
Sbjct: 195 TDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVYSALA 254

Query: 263 DAGYRKMEVIVTETGWASRGDENEAAAT-------------------------------- 314
            AG++ + V ++ETGW S+GD++E  AT                                
Sbjct: 255 AAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPNNDLS 314

BLAST of Csor.00g085730 vs. ExPASy Swiss-Prot
Match: Q9ZQG9 (Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500 PE=2 SV=2)

HSP 1 Score: 255.0 bits (650), Expect = 1.3e-66
Identity = 128/321 (39.88%), Postives = 188/321 (58.57%), Query Frame = 0

Query: 38  GINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKE 97
           GINYG+IA+N+PSP  V  LL++  I  V++YDAD NVL +FS + ++  +GL N +L+ 
Sbjct: 30  GINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQN 89

Query: 98  TSASEEHAMNWVKENVQAFLPETHICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSL 157
            S     A +W+++ ++  + +T I  I +GNE+    D  L  +LL A K++Y A+ +L
Sbjct: 90  MSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTNL 149

Query: 158 NLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAY 217
            L+  V +T+AHS  + S S+PPSS  F+E  +QY++PLL+F ++I SPF +NAYPF AY
Sbjct: 150 GLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFAY 209

Query: 218 MSDPENIDINYALFESTKGIIDPKTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETG 277
              P+ + + Y LF+  +G++DP T LHYDNML AQ+DA Y+A+   G+  +EV ++ETG
Sbjct: 210 KDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISETG 269

Query: 278 WASRGDENEAAAT-------------------------------------------GPTS 316
           W S+GDENE  A+                                           GP S
Sbjct: 270 WPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPVS 329

BLAST of Csor.00g085730 vs. ExPASy Swiss-Prot
Match: Q9FHX5 (Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100 PE=2 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 5.9e-64
Identity = 135/352 (38.35%), Postives = 195/352 (55.40%), Query Frame = 0

Query: 19  VFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHNVLKA 78
           +FS    +  ++     + GINYG++A+N+P P  V+ LLK+     V++YDAD   L+A
Sbjct: 9   LFSLFCLALFSLPLIVSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALRA 68

Query: 79  FSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPETHICGIAIGNEVLGGGDLE 138
           F+G+G EL V L N +L + S     A  WVKENVQA+LP T I  I +GNEVL      
Sbjct: 69  FAGSGFELTVALGNEYLAQMS-DPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSA 128

Query: 139 LWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMRPLLE 198
           L  AL  A ++I+ A+    L+  + +TTAHS A+   S+PPS+  FR +++  + P+L+
Sbjct: 129 LTAALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILD 188

Query: 199 FLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDPKTGLHYDNMLDAQIDAAY 258
           F  + GSP  +NAYPF AY  +P+++ +++ LF+  +G  DP +  HYDNML AQ+DA Y
Sbjct: 189 FHVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVY 248

Query: 259 AALADAG--YRKMEVIVTETGWASRGDENEAAAT-------------------------- 318
            AL   G  Y+K+ ++V+ETGW S GD  E  AT                          
Sbjct: 249 HALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRP 308

Query: 319 ------------------GPTSERNFGLFKADGSISYDVGFHGLKSSSSDSS 325
                             GPTSERN+GLF  DG+  Y +   G+K+SS+ SS
Sbjct: 309 ECDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSL---GIKTSSTHSS 356

BLAST of Csor.00g085730 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 244.2 bits (622), Expect = 2.2e-63
Identity = 129/330 (39.09%), Postives = 185/330 (56.06%), Query Frame = 0

Query: 38  GINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKE 97
           G+NYG++ADN+P P E V LL++  I+ VR+Y AD  ++KA +GTG+ + +G  NG +  
Sbjct: 27  GVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDVPS 86

Query: 98  TSASEEHAMNWVKENVQAFLPETHICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSL 157
            ++    A  W+  NV  F P + I  I +GNE+L   D  L   LL A +N+ KA++++
Sbjct: 87  LASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALEAV 146

Query: 158 NLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAY 217
           +L G ++++T +S  V  +S PPSS  F       ++ +L+FL++ GSPF +N YPF AY
Sbjct: 147 SLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGLKGILQFLSDTGSPFAINPYPFFAY 206

Query: 218 MSDPENIDINYALFESTKGIIDPKTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETG 277
            SDP    + + LFE   G +D KTG+ Y NM DAQ+DA ++AL   G+ K+E++V ETG
Sbjct: 207 QSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVAETG 266

Query: 278 WASRGDENEAAAT-------------------------------------------GPTS 325
           WASRGD NE  A+                                           GP+S
Sbjct: 267 WASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPGPSS 326

BLAST of Csor.00g085730 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 5.7e-59
Identity = 130/343 (37.90%), Postives = 185/343 (53.94%), Query Frame = 0

Query: 16  FWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHNV 75
           FWI  S +A             GI YGR ADN+PSP+ V  L++   IK VRIYDA+ +V
Sbjct: 9   FWIFVSILAFLNF---GMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDV 68

Query: 76  LKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPETHICGIAIGNEVLGGG 135
           LKAF+ TG+EL +G+PN  L   +  + +   W+  N+  + P T I  I++G EV    
Sbjct: 69  LKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAP 128

Query: 136 DLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMRP 195
           D    G +L A +NI+ A+K   LD  ++I+++HS A+ S SFPPSS  F +    +++P
Sbjct: 129 D-NATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKP 188

Query: 196 LLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDPKTGLHYDNMLDAQID 255
           +LEFL E  SPF ++ YP+ AY    E + + YALFES+  ++DP TGL Y NM DAQ+D
Sbjct: 189 MLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLD 248

Query: 256 AAYAALADAGYRKMEVIVTETGWASRGDENEAAAT------------------------- 315
           A Y AL    ++ ++V+VTE+GW S+G   E AAT                         
Sbjct: 249 AIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKP 308

BLAST of Csor.00g085730 vs. NCBI nr
Match: KAG6599392.1 (Glucan endo-1,3-beta-glucosidase 11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 701 bits (1808), Expect = 2.77e-254
Identity = 356/356 (100.00%), Postives = 356/356 (100.00%), Query Frame = 0

Query: 1   MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120
           AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET
Sbjct: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240
           SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP
Sbjct: 181 SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240

Query: 241 KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAATGPTSERNFGL 300
           KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAATGPTSERNFGL
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAATGPTSERNFGL 300

Query: 301 FKADGSISYDVGFHGLKSSSSDSSYLSLKSFRARGLSANHIFSLTFSAVTLLLLLR 356
           FKADGSISYDVGFHGLKSSSSDSSYLSLKSFRARGLSANHIFSLTFSAVTLLLLLR
Sbjct: 301 FKADGSISYDVGFHGLKSSSSDSSYLSLKSFRARGLSANHIFSLTFSAVTLLLLLR 356

BLAST of Csor.00g085730 vs. NCBI nr
Match: XP_022946068.1 (glucan endo-1,3-beta-glucosidase 11-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 677 bits (1746), Expect = 3.89e-244
Identity = 354/399 (88.72%), Postives = 355/399 (88.97%), Query Frame = 0

Query: 1   MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120
           AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET
Sbjct: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDG VQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGAVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240
           SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP
Sbjct: 181 SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240

Query: 241 KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAAT---------- 300
           KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAAT          
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAATVSNAKIFNYN 300

Query: 301 ---------------------------------GPTSERNFGLFKADGSISYDVGFHGLK 356
                                            GPTSERNFGLFKADGSISYDVGFHGLK
Sbjct: 301 LRKRLAKKKGTPYRPRNVLKAYVFATFNENLKPGPTSERNFGLFKADGSISYDVGFHGLK 360

BLAST of Csor.00g085730 vs. NCBI nr
Match: XP_022999162.1 (glucan endo-1,3-beta-glucosidase 14-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 669 bits (1726), Expect = 4.34e-241
Identity = 351/399 (87.97%), Postives = 352/399 (88.22%), Query Frame = 0

Query: 1   MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKCFWLCV SLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWLCVQSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120
           AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET
Sbjct: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240
           SSCIFRENVVQYMRPLLEFL EIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP
Sbjct: 181 SSCIFRENVVQYMRPLLEFLAEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240

Query: 241 KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAAT---------- 300
           KT LHYDNMLDAQIDAAYAALADAGY+KMEVIVTETGWASRGDENEAAAT          
Sbjct: 241 KTDLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATVSNAKIFNYN 300

Query: 301 ---------------------------------GPTSERNFGLFKADGSISYDVGFHGLK 356
                                            GPTSERNFGLFKADGSISYDVGFHGLK
Sbjct: 301 LRKRLAKKKGTPYRPRNVLKAYVFATFNENLKQGPTSERNFGLFKADGSISYDVGFHGLK 360

BLAST of Csor.00g085730 vs. NCBI nr
Match: XP_023545837.1 (glucan endo-1,3-beta-glucosidase 11-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 664 bits (1712), Expect = 5.47e-239
Identity = 351/399 (87.97%), Postives = 351/399 (87.97%), Query Frame = 0

Query: 1   MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKCFWLCV SLPGFWIVFSAIASST  VQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWLCVQSLPGFWIVFSAIASST--VQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120
           AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET
Sbjct: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240
           SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP
Sbjct: 181 SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240

Query: 241 KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAAT---------- 300
           KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAAT          
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAATVSNAKIFNYN 300

Query: 301 ---------------------------------GPTSERNFGLFKADGSISYDVGFHGLK 356
                                            GPTSERNFGLFKADGSISYDVGFHGLK
Sbjct: 301 LRKRLAKKKGTPYRPRNVLKAYVFATFNENLKPGPTSERNFGLFKADGSISYDVGFHGLK 360

BLAST of Csor.00g085730 vs. NCBI nr
Match: XP_008457422.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 11 [Cucumis melo])

HSP 1 Score: 641 bits (1654), Expect = 4.03e-230
Identity = 333/399 (83.46%), Postives = 343/399 (85.96%), Query Frame = 0

Query: 1   MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKCFW C  S+PGFW+VFSAIAS TMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWFCFQSIPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120
           AKIKNVRIYDADH VLKAFSGTGLEL VGLPNGFL+E SASEEHAMNWVKENVQAFLPET
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLREMSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVK+LNLDGVVQITTAHSQAVF NSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKNLNLDGVVQITTAHSQAVFWNSFPP 180

Query: 181 SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240
           SSCIFRENVVQYM+PLLEFL+EIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGI DP
Sbjct: 181 SSCIFRENVVQYMKPLLEFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAAT---------- 300
           KTGLHYDNMLDAQIDAAYAALADAGY+KMEVIVTETGWASRGDENEAAAT          
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATASNARIFNYN 300

Query: 301 ---------------------------------GPTSERNFGLFKADGSISYDVGFHGLK 356
                                            GPTSERNFGLFKADGSISYD+GFHGLK
Sbjct: 301 LRKRLAKKKGTPFRPKNALKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360

BLAST of Csor.00g085730 vs. ExPASy TrEMBL
Match: A0A6J1G2S4 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450268 PE=3 SV=1)

HSP 1 Score: 677 bits (1746), Expect = 1.88e-244
Identity = 354/399 (88.72%), Postives = 355/399 (88.97%), Query Frame = 0

Query: 1   MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120
           AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET
Sbjct: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDG VQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGAVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240
           SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP
Sbjct: 181 SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240

Query: 241 KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAAT---------- 300
           KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAAT          
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAATVSNAKIFNYN 300

Query: 301 ---------------------------------GPTSERNFGLFKADGSISYDVGFHGLK 356
                                            GPTSERNFGLFKADGSISYDVGFHGLK
Sbjct: 301 LRKRLAKKKGTPYRPRNVLKAYVFATFNENLKPGPTSERNFGLFKADGSISYDVGFHGLK 360

BLAST of Csor.00g085730 vs. ExPASy TrEMBL
Match: A0A6J1KA33 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493627 PE=3 SV=1)

HSP 1 Score: 669 bits (1726), Expect = 2.10e-241
Identity = 351/399 (87.97%), Postives = 352/399 (88.22%), Query Frame = 0

Query: 1   MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKCFWLCV SLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWLCVQSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120
           AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET
Sbjct: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240
           SSCIFRENVVQYMRPLLEFL EIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP
Sbjct: 181 SSCIFRENVVQYMRPLLEFLAEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240

Query: 241 KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAAT---------- 300
           KT LHYDNMLDAQIDAAYAALADAGY+KMEVIVTETGWASRGDENEAAAT          
Sbjct: 241 KTDLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATVSNAKIFNYN 300

Query: 301 ---------------------------------GPTSERNFGLFKADGSISYDVGFHGLK 356
                                            GPTSERNFGLFKADGSISYDVGFHGLK
Sbjct: 301 LRKRLAKKKGTPYRPRNVLKAYVFATFNENLKQGPTSERNFGLFKADGSISYDVGFHGLK 360

BLAST of Csor.00g085730 vs. ExPASy TrEMBL
Match: A0A1S3C5G6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497110 PE=3 SV=1)

HSP 1 Score: 641 bits (1654), Expect = 1.95e-230
Identity = 333/399 (83.46%), Postives = 343/399 (85.96%), Query Frame = 0

Query: 1   MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKCFW C  S+PGFW+VFSAIAS TMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWFCFQSIPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120
           AKIKNVRIYDADH VLKAFSGTGLEL VGLPNGFL+E SASEEHAMNWVKENVQAFLPET
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLREMSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVK+LNLDGVVQITTAHSQAVF NSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKNLNLDGVVQITTAHSQAVFWNSFPP 180

Query: 181 SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240
           SSCIFRENVVQYM+PLLEFL+EIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGI DP
Sbjct: 181 SSCIFRENVVQYMKPLLEFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAAT---------- 300
           KTGLHYDNMLDAQIDAAYAALADAGY+KMEVIVTETGWASRGDENEAAAT          
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATASNARIFNYN 300

Query: 301 ---------------------------------GPTSERNFGLFKADGSISYDVGFHGLK 356
                                            GPTSERNFGLFKADGSISYD+GFHGLK
Sbjct: 301 LRKRLAKKKGTPFRPKNALKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360

BLAST of Csor.00g085730 vs. ExPASy TrEMBL
Match: A0A0A0LF78 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G006850 PE=3 SV=1)

HSP 1 Score: 630 bits (1626), Expect = 3.59e-226
Identity = 328/399 (82.21%), Postives = 339/399 (84.96%), Query Frame = 0

Query: 1   MARKCFWLCVLSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKCFW C  SL GFW+VFSAIAS TMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWFCFQSLSGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120
           AKIKNVRIYDADH VLKAFSGTGLEL VGLPNGFL+E SASEEHAMNWVKENVQAFLPET
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLREMSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWG LLGAAKNIYKAVK+LNLDGVVQITTAHSQAVF NSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGTLLGAAKNIYKAVKNLNLDGVVQITTAHSQAVFWNSFPP 180

Query: 181 SSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240
           SSCIFRENVVQYM+PLLEFL+EIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGI DP
Sbjct: 181 SSCIFRENVVQYMKPLLEFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAAT---------- 300
           KTGLHYDNMLDAQIDAAYAALADAGY+KMEVIVTETGWASRGDENEAAAT          
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATASNARIFNYN 300

Query: 301 ---------------------------------GPTSERNFGLFKADGSISYDVGFHGLK 356
                                            GPTSERNFGLFKADGSISYD+GFHGLK
Sbjct: 301 LRKRLAKKKGTPFRPKNALKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360

BLAST of Csor.00g085730 vs. ExPASy TrEMBL
Match: A0A6J1KIU2 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493627 PE=3 SV=1)

HSP 1 Score: 618 bits (1593), Expect = 1.41e-221
Identity = 325/372 (87.37%), Postives = 326/372 (87.63%), Query Frame = 0

Query: 28  MTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHNVLKAFSGTGLELE 87
           MTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHNVLKAFSGTGLELE
Sbjct: 1   MTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHNVLKAFSGTGLELE 60

Query: 88  VGLPNGFLKETSASEEHAMNWVKENVQAFLPETHICGIAIGNEVLGGGDLELWGALLGAA 147
           VGLPNGFLKETSASEEHAMNWVKENVQAFLPETHICGIAIGNEVLGGGDLELWGALLGAA
Sbjct: 61  VGLPNGFLKETSASEEHAMNWVKENVQAFLPETHICGIAIGNEVLGGGDLELWGALLGAA 120

Query: 148 KNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMRPLLEFLTEIGSPF 207
           KNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMRPLLEFL EIGSPF
Sbjct: 121 KNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMRPLLEFLAEIGSPF 180

Query: 208 CLNAYPFLAYMSDPENIDINYALFESTKGIIDPKTGLHYDNMLDAQIDAAYAALADAGYR 267
           CLNAYPFLAYMSDPENIDINYALFESTKGIIDPKT LHYDNMLDAQIDAAYAALADAGY+
Sbjct: 181 CLNAYPFLAYMSDPENIDINYALFESTKGIIDPKTDLHYDNMLDAQIDAAYAALADAGYQ 240

Query: 268 KMEVIVTETGWASRGDENEAAAT------------------------------------- 327
           KMEVIVTETGWASRGDENEAAAT                                     
Sbjct: 241 KMEVIVTETGWASRGDENEAAATVSNAKIFNYNLRKRLAKKKGTPYRPRNVLKAYVFATF 300

Query: 328 ------GPTSERNFGLFKADGSISYDVGFHGLKSSSSDSSYLSLKSFRARGLSANHIFSL 356
                 GPTSERNFGLFKADGSISYDVGFHGLKSSSSDSSYLSLKSFRARGL ANHIFSL
Sbjct: 301 NENLKQGPTSERNFGLFKADGSISYDVGFHGLKSSSSDSSYLSLKSFRARGLLANHIFSL 360

BLAST of Csor.00g085730 vs. TAIR 10
Match: AT2G26600.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 436.4 bits (1121), Expect = 2.2e-122
Identity = 232/390 (59.49%), Postives = 277/390 (71.03%), Query Frame = 0

Query: 10  VLSLPGFWIVFSAIASSTMTVQ-AFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRI 69
           +L L  F+  F  + S    V  AF GTYGINYGRIADNIPSP++VV LLK AKI+NVRI
Sbjct: 6   LLLLKFFFFFFFNLTSLEYQVDGAFVGTYGINYGRIADNIPSPEKVVLLLKQAKIRNVRI 65

Query: 70  YDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPETHICGIAIG 129
           YD DH VL+AFSGTGL+L VGLPNGFLKE S++ +HA +WVKEN+Q+FLP+T I GIAIG
Sbjct: 66  YDVDHTVLEAFSGTGLDLVVGLPNGFLKEMSSNADHAFSWVKENIQSFLPKTRIRGIAIG 125

Query: 130 NEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPPSSCIFREN 189
           NEVLGGGD EL GALLGAAKN+Y A+K +NL+  VQITTAHSQAVFS+S+PPSSC+F+EN
Sbjct: 126 NEVLGGGDSELAGALLGAAKNVYNALKKMNLEDTVQITTAHSQAVFSDSYPPSSCVFKEN 185

Query: 190 VVQYMRPLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDPKTGLHYDN 249
           VVQ+M+PLLEF  +IGSPFCLNAYPFLAY  +P+ IDINYALF+ T+GI DPKT LHYDN
Sbjct: 186 VVQFMKPLLEFFQQIGSPFCLNAYPFLAYTYNPKEIDINYALFKPTEGIYDPKTDLHYDN 245

Query: 250 MLDAQIDAAYAALADAGYRKMEVIVTETGWASRGDENEAAAT------------------ 309
           MLDAQIDAAY AL DAG++KMEV++TETGWAS+GD +E AAT                  
Sbjct: 246 MLDAQIDAAYMALQDAGFKKMEVMITETGWASKGDSDEPAATPENARTYNYNLRKRLAKK 305

Query: 310 -------------------------GPTSERNFGLFKADGSISYDVGFHGLKSSSSDSSY 356
                                    G +SE +FGLFK DG+ISYD+GF+ LKS S  S  
Sbjct: 306 KGTPLRPKTVLKAYIFALFNENSKPGKSSETHFGLFKPDGTISYDIGFNNLKSDSPKSLI 365

BLAST of Csor.00g085730 vs. TAIR 10
Match: AT3G15800.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 387.5 bits (994), Expect = 1.2e-107
Identity = 205/378 (54.23%), Postives = 256/378 (67.72%), Query Frame = 0

Query: 16  FWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHNV 75
           F +VFS ++S T    AF GTYG+NYGRIADN+PSPD V +LLK+AKI+N RIYDADH+V
Sbjct: 24  FLLVFSILSSQTAV--AFIGTYGVNYGRIADNLPSPDAVATLLKSAKIRNTRIYDADHSV 83

Query: 76  LKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFL-PETHICGIAIGNEVLGG 135
           L AF GTG+E+ VGL N FLK+ S  E+ AMNW+KENV+ F+   T I GIA+GNE+LGG
Sbjct: 84  LTAFRGTGIEIIVGLGNEFLKDISVGEDRAMNWIKENVEPFIRGGTKISGIAVGNEILGG 143

Query: 136 GDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMR 195
            D+ LW ALL AAKN+Y A++ L L  VV++++ HS+AVF+NS+PPSSC FR++V  +M+
Sbjct: 144 TDIGLWEALLPAAKNVYSALRRLGLHNVVEVSSPHSEAVFANSYPPSSCTFRDDVAPFMK 203

Query: 196 PLLEFLTEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDPKTGLHYDNMLDAQI 255
           PLL F  +I SPF +NAYPFLAY SDP  IDINYALFE  KGI+DPKT LHYDNM DA +
Sbjct: 204 PLLAFFWQIQSPFYINAYPFLAYKSDPITIDINYALFEHNKGILDPKTKLHYDNMFDAMV 263

Query: 256 DAAYAALADAGYRKMEVIVTETGWASRGDENEAAAT------------------------ 315
           DA+YAAL  AGY K+ VIV+ETGWAS+GD +E  A+                        
Sbjct: 264 DASYAALEKAGYTKVPVIVSETGWASKGDADEPGASVKNARTYNRNLRKRLQKRKGTPYR 323

Query: 316 -------------------GPTSERNFGLFKADGSISYDVGFHGLKSSSS-----DSSYL 345
                              GPTSERNFGLFK DG+I+YD+G  GLKSSS+      SS +
Sbjct: 324 PDMVVRAYVFALFNENSKPGPTSERNFGLFKPDGTIAYDIGLTGLKSSSATRYRFKSSLV 383

BLAST of Csor.00g085730 vs. TAIR 10
Match: AT2G26600.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 324.3 bits (830), Expect = 1.2e-88
Identity = 171/300 (57.00%), Postives = 208/300 (69.33%), Query Frame = 0

Query: 99  SASEEHAMNWVKENVQAFLPETHICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLN 158
           S++ +HA +WVKEN+Q+FLP+T I GIAIGNEVLGGGD EL GALLGAAKN+Y A+K +N
Sbjct: 2   SSNADHAFSWVKENIQSFLPKTRIRGIAIGNEVLGGGDSELAGALLGAAKNVYNALKKMN 61

Query: 159 LDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAYM 218
           L+  VQITTAHSQAVFS+S+PPSSC+F+ENVVQ+M+PLLEF  +IGSPFCLNAYPFLAY 
Sbjct: 62  LEDTVQITTAHSQAVFSDSYPPSSCVFKENVVQFMKPLLEFFQQIGSPFCLNAYPFLAYT 121

Query: 219 SDPENIDINYALFESTKGIIDPKTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETGW 278
            +P+ IDINYALF+ T+GI DPKT LHYDNMLDAQIDAAY AL DAG++KMEV++TETGW
Sbjct: 122 YNPKEIDINYALFKPTEGIYDPKTDLHYDNMLDAQIDAAYMALQDAGFKKMEVMITETGW 181

Query: 279 ASRGDENEAAAT-------------------------------------------GPTSE 338
           AS+GD +E AAT                                           G +SE
Sbjct: 182 ASKGDSDEPAATPENARTYNYNLRKRLAKKKGTPLRPKTVLKAYIFALFNENSKPGKSSE 241

Query: 339 RNFGLFKADGSISYDVGFHGLKSSSSDSSYLSLKSFRARGLSANHIFSLTFSAVTLLLLL 356
            +FGLFK DG+ISYD+GF+ LKS S  S   S K       SA +  +L  S    LL++
Sbjct: 242 THFGLFKPDGTISYDIGFNNLKSDSPKSLISSSK-------SARYYVALVISVSAFLLMI 294

BLAST of Csor.00g085730 vs. TAIR 10
Match: AT1G32860.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 261.5 bits (667), Expect = 9.6e-70
Identity = 132/336 (39.29%), Postives = 198/336 (58.93%), Query Frame = 0

Query: 23  IASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHNVLKAFSGT 82
           I+ + + +   T + G+NYG+I DN+PSP +V+ L+K+     V++YDA+  +LKAFS T
Sbjct: 15  ISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILKAFSNT 74

Query: 83  GLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPETHICGIAIGNEVLGGGDLELWGA 142
           G+E  +GL N +L +       A+ W+K+NV  FLP T+I  I IGNE+L   D  L   
Sbjct: 75  GIEFIIGLGNEYLSKMK-DPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSSLTTN 134

Query: 143 LLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMRPLLEFLTE 202
           LL A + ++ A+ +  L   + +TTAHS ++  +SFPPS+  F+ +++  + P+LEF  +
Sbjct: 135 LLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILEFHRK 194

Query: 203 IGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDPKTGLHYDNMLDAQIDAAYAALA 262
             SPF +NAYPF AY  +P+ + +++ LF+  +GI+DP TG HYDNML AQIDA Y+ALA
Sbjct: 195 TDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVYSALA 254

Query: 263 DAGYRKMEVIVTETGWASRGDENEAAAT-------------------------------- 314
            AG++ + V ++ETGW S+GD++E  AT                                
Sbjct: 255 AAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPNNDLS 314

BLAST of Csor.00g085730 vs. TAIR 10
Match: AT2G27500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 255.0 bits (650), Expect = 9.0e-68
Identity = 128/321 (39.88%), Postives = 188/321 (58.57%), Query Frame = 0

Query: 38  GINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKE 97
           GINYG+IA+N+PSP  V  LL++  I  V++YDAD NVL +FS + ++  +GL N +L+ 
Sbjct: 30  GINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQN 89

Query: 98  TSASEEHAMNWVKENVQAFLPETHICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSL 157
            S     A +W+++ ++  + +T I  I +GNE+    D  L  +LL A K++Y A+ +L
Sbjct: 90  MSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTNL 149

Query: 158 NLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMRPLLEFLTEIGSPFCLNAYPFLAY 217
            L+  V +T+AHS  + S S+PPSS  F+E  +QY++PLL+F ++I SPF +NAYPF AY
Sbjct: 150 GLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFAY 209

Query: 218 MSDPENIDINYALFESTKGIIDPKTGLHYDNMLDAQIDAAYAALADAGYRKMEVIVTETG 277
              P+ + + Y LF+  +G++DP T LHYDNML AQ+DA Y+A+   G+  +EV ++ETG
Sbjct: 210 KDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISETG 269

Query: 278 WASRGDENEAAAT-------------------------------------------GPTS 316
           W S+GDENE  A+                                           GP S
Sbjct: 270 WPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPVS 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L8681.4e-6839.29Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860... [more]
Q9ZQG91.3e-6639.88Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500... [more]
Q9FHX55.9e-6438.35Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100... [more]
Q9M0692.2e-6339.09Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q8VYE55.7e-5937.90Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Match NameE-valueIdentityDescription
KAG6599392.12.77e-254100.00Glucan endo-1,3-beta-glucosidase 11, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_022946068.13.89e-24488.72glucan endo-1,3-beta-glucosidase 11-like isoform X1 [Cucurbita moschata][more]
XP_022999162.14.34e-24187.97glucan endo-1,3-beta-glucosidase 14-like isoform X1 [Cucurbita maxima][more]
XP_023545837.15.47e-23987.97glucan endo-1,3-beta-glucosidase 11-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_008457422.14.03e-23083.46PREDICTED: glucan endo-1,3-beta-glucosidase 11 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1G2S41.88e-24488.72(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450268 P... [more]
A0A6J1KA332.10e-24187.97(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493627 PE=... [more]
A0A1S3C5G61.95e-23083.46(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497110 PE=3 SV... [more]
A0A0A0LF783.59e-22682.21(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G006850 PE=3... [more]
A0A6J1KIU21.41e-22187.37(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493627 PE=... [more]
Match NameE-valueIdentityDescription
AT2G26600.12.2e-12259.49Glycosyl hydrolase superfamily protein [more]
AT3G15800.11.2e-10754.23Glycosyl hydrolase superfamily protein [more]
AT2G26600.21.2e-8857.00Glycosyl hydrolase superfamily protein [more]
AT1G32860.19.6e-7039.29Glycosyl hydrolase superfamily protein [more]
AT2G27500.19.0e-6839.88Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 38..284
e-value: 4.1E-59
score: 200.7
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 37..293
e-value: 1.1E-88
score: 299.2
NoneNo IPR availablePANTHERPTHR32227:SF129GLYCOSYL HYDROLASE SUPERFAMILY PROTEINcoord: 26..290
coord: 290..348
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 290..348
coord: 26..290
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 37..312

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g085730.m01Csor.00g085730.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds