Csor.00g085370 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g085370
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description(1->3)-beta-glucan endohydrolase
LocationCsor_Chr05: 10593108 .. 10594714 (-)
RNA-Seq ExpressionCsor.00g085370
SyntenyCsor.00g085370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGGAATCTGTGTTACGATTCCACAGAAATTGGATATTCAGAGCTTTGATTTTGTTGTTGTTGACGGTTGTTCCGAATGGATTTAGTGTTGGAGTGAACTGGGGAACTATGGCTACTCACCAGCTTCCTCCAGAGAAAGTTGTTAAAATGCTTCAAGAAAATGGGTTTCGTAAGTTGAAGCTTTTTGAAGCTGAGGATCGGATTTTGGAAGCCCTAATTGGGACTGATATTGAGGTTATGTTGGCTATTCCTAATTCAATGTTGCAGGTTATGAGTGAAGATCCTGAGGCTGCAGCTCTTTGGGTGGATTCTAATGTTACTGCTTACACTTATCATGGTGGTGTTAAAATCAAGTTGAGTATTGATTTACTTTGGTTTTTGTTCTTCGTTTTTTTTTTTGTGCTCGTAAGGTGTTCGATGAAATTCCCACTTCATGTTTCTCTGTTTTGAATGCGTTCTTTTCAAGCCTGGCTTTGAAACTTCACATGAAATCTTTAGAAAGCGATTGAATTGGCCAAAAATAAGTTTAGCTCATCTTTTGTTTTAGAAAGTGATTGAATTGTCCTCTCTAGGCTTTCCTTTTCGAGTTTCCCCTCAAGGTTTTTAAAACGCGTATACTAGGGAGAGGTTTTCACATCGTTATAAAGAATGCTCTGTTACCCTCTCCAATCGATGTGGGATCTCACAATAATCCACCCCCTTCAGGGCCCAGCATCCTCGCTGACACTTGTTCCCTTCTCAAATCGATGTGGGAATCTAATAATCCATCCATTTTCAGGGCTCACCATCCTTGGTAGCACACTGCTCAGTGTCTGGCTTTGATACTATTTGTTACAGCCTAAGTCCACCGCTAGCAGACATTGTTCTCTTTGAATTCTCCCTTTCGGGCTTTCTCTCAAAGTTTTTAAAACACATCTGCTAGGGAGAGATTTCCACACCCTACAAACAATGTTTTGTTCTTCTCTCCAACCGATGTGGGATCTCACTACAATCCACCCTGTTTGGGGTCTAGCGTCCTTTTCACACCATTATAAAGAATGTTTCGTTCTTCTCTCCAACCGATGTGGGTTCTCACAATTATCCACCCCCTTTGGGGCCTAACGTCTTTTCCACACTCTTATAAAGAATGTTTCATTCCCCTCTTCAACTGATATGGAAACTCACAATATTCCACCCCTTCGGGGCCCAGTGTCTTCACTGGCGCTCATTCCTCTCTCCAATCGATTTGGGATCTCATAGCAACCTCCTGTACCGAAAGAATTGAAATTTGTTAATGCATTTTTAGAAGTTTAAGAACCGGATTTTACGAATGTTAAGTTAGCTTAACAAGCATATTGGTTCTTTCCATCTCCTATAAACTTCCATTGCAAACTGATTTTGCAGGTATGTTGCTGTAGGCAATGAGCCCTTTCTTAAGTCCTACAACGGCATCTATCTTCAGTCAACCTTACCTGCCCTGAGGAATATCCAGCAAGCCTTAAACGATGCAGGACTTGGATCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATACTTACAATTCCCCGGACTCAAACCCGGTTCCATCTGCAGGCGATTTTCGGCCAGACGTCCGAGAATTAA

mRNA sequence

ATGTCGGAATCTGTGTTACGATTCCACAGAAATTGGATATTCAGAGCTTTGATTTTGTTGTTGTTGACGGTTGTTCCGAATGGATTTAGTGTTGGAGTGAACTGGGGAACTATGGCTACTCACCAGCTTCCTCCAGAGAAAGTTGTTAAAATGCTTCAAGAAAATGGGTTTCGTAAGTTGAAGCTTTTTGAAGCTGAGGATCGGATTTTGGAAGCCCTAATTGGGACTGATATTGAGGTTATGTTGGCTATTCCTAATTCAATGTTGCAGGTTATGAGTGAAGATCCTGAGGCTGCAGCTCTTTGGGTGGATTCTAATGTTACTGCTTACACTTATCATGGTGGTGTTAAAATCAAGTATGTTGCTGTAGGCAATGAGCCCTTTCTTAAGTCCTACAACGGCATCTATCTTCAGTCAACCTTACCTGCCCTGAGGAATATCCAGCAAGCCTTAAACGATGCAGGACTTGGATCAAAGGCGATTTTCGGCCAGACGTCCGAGAATTAA

Coding sequence (CDS)

ATGTCGGAATCTGTGTTACGATTCCACAGAAATTGGATATTCAGAGCTTTGATTTTGTTGTTGTTGACGGTTGTTCCGAATGGATTTAGTGTTGGAGTGAACTGGGGAACTATGGCTACTCACCAGCTTCCTCCAGAGAAAGTTGTTAAAATGCTTCAAGAAAATGGGTTTCGTAAGTTGAAGCTTTTTGAAGCTGAGGATCGGATTTTGGAAGCCCTAATTGGGACTGATATTGAGGTTATGTTGGCTATTCCTAATTCAATGTTGCAGGTTATGAGTGAAGATCCTGAGGCTGCAGCTCTTTGGGTGGATTCTAATGTTACTGCTTACACTTATCATGGTGGTGTTAAAATCAAGTATGTTGCTGTAGGCAATGAGCCCTTTCTTAAGTCCTACAACGGCATCTATCTTCAGTCAACCTTACCTGCCCTGAGGAATATCCAGCAAGCCTTAAACGATGCAGGACTTGGATCAAAGGCGATTTTCGGCCAGACGTCCGAGAATTAA

Protein sequence

MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSKAIFGQTSEN
Homology
BLAST of Csor.00g085370 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 156.4 bits (394), Expect = 2.9e-37
Identity = 78/137 (56.93%), Postives = 103/137 (75.18%), Query Frame = 0

Query: 22  LTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALIGTDIEVM 81
           L ++ NG  +GVNWGTMATH+LPP+ VV+ML++N   K+KLF+A++  + AL G+ +EVM
Sbjct: 18  LGILVNG--LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVM 77

Query: 82  LAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGIYLQSTL 141
           +AIPN  L+VM+   + A  WV  NVT Y + GGV I +VAVGNEPFLKSYNG ++  T 
Sbjct: 78  VAIPNDQLKVMT-SYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTF 137

Query: 142 PALRNIQQALNDAGLGS 159
           PAL NIQ ALN+AGLG+
Sbjct: 138 PALANIQNALNEAGLGN 151

BLAST of Csor.00g085370 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.5e-33
Identity = 72/147 (48.98%), Postives = 101/147 (68.71%), Query Frame = 0

Query: 13  IFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEA 72
           +F  + LL  +++     +GVNWG+ A H LPP  VV++L+ENG +K+KLFEA+  IL+A
Sbjct: 9   VFFVITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKA 68

Query: 73  LIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSY 132
           L  T I+VM+ IPN +L  ++    AA  WV  NV+A+    GV I+YVAVGNEPFLK++
Sbjct: 69  LSRTGIQVMVGIPNDLLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAF 128

Query: 133 NGIYLQSTLPALRNIQQALNDAGLGSK 160
           NG +   TLPAL+NIQ A+  AGL ++
Sbjct: 129 NGTFEGITLPALQNIQSAIIKAGLATQ 155

BLAST of Csor.00g085370 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 142.9 bits (359), Expect = 3.3e-33
Identity = 74/145 (51.03%), Postives = 96/145 (66.21%), Query Frame = 0

Query: 12  WIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILE 71
           W    L+L +  +     S+G NWGT A+H LPP+ VV+ML+ENG +K+KLF+AE   L 
Sbjct: 3   WGSVLLLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDTLR 62

Query: 72  ALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKS 131
           AL  + IEVM+ IPN ML  ++   +AA  WV  NV+ +     V I+YVAVGNEPFL +
Sbjct: 63  ALGKSGIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNEPFLST 122

Query: 132 YNGIYLQSTLPALRNIQQALNDAGL 157
           YNG YL +T PALRNIQ A+  AGL
Sbjct: 123 YNGSYLSTTFPALRNIQIAIIKAGL 147

BLAST of Csor.00g085370 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.6e-27
Identity = 73/155 (47.10%), Postives = 98/155 (63.23%), Query Frame = 0

Query: 15  RALILLLLTVVPNGFS--------VGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAE 74
           R L LLLL V   G S        VG+NWGT A+H LPP KVV++L+ NG  K+KLF+A+
Sbjct: 3   RRLFLLLLAVTA-GLSLTGTTVRAVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDAD 62

Query: 75  DRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGG--VKIKYVAVG 134
            ++L AL G++I V + I NSML+ ++   + A  WV  NVT Y ++GG  V+I+YVAVG
Sbjct: 63  PKVLRALSGSNIGVTIGIQNSMLKSLNASVKVAESWVHDNVTRY-FNGGNRVRIEYVAVG 122

Query: 135 NEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 160
            EPFL+SY   Y    + A  NIQ AL  A L ++
Sbjct: 123 EEPFLQSYGNQYKPFVIGAAMNIQNALVKANLANE 155

BLAST of Csor.00g085370 vs. ExPASy Swiss-Prot
Match: A7PQW3 (Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120 PE=1 SV=2)

HSP 1 Score: 97.4 bits (241), Expect = 1.6e-19
Identity = 54/151 (35.76%), Postives = 85/151 (56.29%), Query Frame = 0

Query: 10  RNWIFRALILL----LLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEA 69
           RN  F  ++LL    + T+     S+GV +G    +     +V+ + + NG   +++++ 
Sbjct: 5   RNRPFVVVLLLGFVIMSTITIGAQSIGVCYGRNGNNLPSASQVINLYKSNGIGSMRIYDP 64

Query: 70  EDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGN 129
               L+AL G+DIE++L +PN+ LQ ++ D  AAA WV +NV    Y   VK +Y+AVGN
Sbjct: 65  NSDTLQALRGSDIELILDVPNTDLQSLASDASAAATWVQNNVV--NYASEVKFRYIAVGN 124

Query: 130 EPFLKSYNGIYLQSTLPALRNIQQALNDAGL 157
           E      N  Y Q  LPA++N+Q A+  AGL
Sbjct: 125 EVLPTGSNAQYAQYVLPAMKNVQSAITSAGL 153

BLAST of Csor.00g085370 vs. NCBI nr
Match: KAG6599427.1 (Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 333 bits (853), Expect = 5.48e-115
Identity = 168/168 (100.00%), Postives = 168/168 (100.00%), Query Frame = 0

Query: 1   MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60
           MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL
Sbjct: 1   MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120
           KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120

Query: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSKAIFGQTSEN 168
           VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSKAIFGQTSEN
Sbjct: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSKAIFGQTSEN 168

BLAST of Csor.00g085370 vs. NCBI nr
Match: XP_023546836.1 (glucan endo-1,3-beta-glucosidase 8-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 315 bits (807), Expect = 2.30e-103
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0

Query: 1   MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60
           MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL
Sbjct: 1   MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120
           KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120

Query: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 159
           VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK
Sbjct: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 159

BLAST of Csor.00g085370 vs. NCBI nr
Match: XP_022946334.1 (glucan endo-1,3-beta-glucosidase 8-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 312 bits (799), Expect = 3.82e-102
Identity = 158/159 (99.37%), Postives = 158/159 (99.37%), Query Frame = 0

Query: 1   MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60
           MSESVLRFHRNWIF ALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL
Sbjct: 1   MSESVLRFHRNWIFIALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120
           KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120

Query: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 159
           VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK
Sbjct: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 159

BLAST of Csor.00g085370 vs. NCBI nr
Match: XP_022999582.1 (glucan endo-1,3-beta-glucosidase 8-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 306 bits (784), Expect = 6.62e-100
Identity = 156/159 (98.11%), Postives = 156/159 (98.11%), Query Frame = 0

Query: 1   MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60
           MSESVLRFHRN IFRALILLLLTVVPNG  VGVNWGTMATHQLPPEKVVKMLQENGFRKL
Sbjct: 1   MSESVLRFHRNCIFRALILLLLTVVPNGLGVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120
           KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120

Query: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 159
           VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK
Sbjct: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 159

BLAST of Csor.00g085370 vs. NCBI nr
Match: KAG7030416.1 (Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 287 bits (734), Expect = 1.98e-92
Identity = 148/159 (93.08%), Postives = 148/159 (93.08%), Query Frame = 0

Query: 1   MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60
           MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL
Sbjct: 1   MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120
           KLFEAEDRILEALIGTDIEVM           SEDPEAAALWVDSNVTAYTYHGGVKIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVM-----------SEDPEAAALWVDSNVTAYTYHGGVKIKY 120

Query: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 159
           VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK
Sbjct: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 148

BLAST of Csor.00g085370 vs. ExPASy TrEMBL
Match: A0A6J1G3H9 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450439 PE=3 SV=1)

HSP 1 Score: 312 bits (799), Expect = 1.85e-102
Identity = 158/159 (99.37%), Postives = 158/159 (99.37%), Query Frame = 0

Query: 1   MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60
           MSESVLRFHRNWIF ALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL
Sbjct: 1   MSESVLRFHRNWIFIALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120
           KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120

Query: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 159
           VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK
Sbjct: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 159

BLAST of Csor.00g085370 vs. ExPASy TrEMBL
Match: A0A6J1KB85 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493909 PE=3 SV=1)

HSP 1 Score: 306 bits (784), Expect = 3.21e-100
Identity = 156/159 (98.11%), Postives = 156/159 (98.11%), Query Frame = 0

Query: 1   MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60
           MSESVLRFHRN IFRALILLLLTVVPNG  VGVNWGTMATHQLPPEKVVKMLQENGFRKL
Sbjct: 1   MSESVLRFHRNCIFRALILLLLTVVPNGLGVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120
           KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120

Query: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 159
           VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK
Sbjct: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 159

BLAST of Csor.00g085370 vs. ExPASy TrEMBL
Match: A0A6J1DUS6 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111024222 PE=3 SV=1)

HSP 1 Score: 274 bits (701), Expect = 1.17e-87
Identity = 139/159 (87.42%), Postives = 146/159 (91.82%), Query Frame = 0

Query: 1   MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60
           MS+S L+ HR+ IF ALILL  T+ PNG SVGVNWGTMATHQLPPEKVVKMLQENGFRKL
Sbjct: 1   MSKSGLQIHRDCIFTALILLYSTIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120
           KLFEAEDRILEALIG+DIEVMLAIPNSMLQ+MSEDPEAAA WVDSNVT Y+YHGGV IKY
Sbjct: 61  KLFEAEDRILEALIGSDIEVMLAIPNSMLQIMSEDPEAAASWVDSNVTTYSYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 159
           VAVGNEPFLKSYNG YLQ TLPALRNIQQALNDAGLGSK
Sbjct: 121 VAVGNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSK 159

BLAST of Csor.00g085370 vs. ExPASy TrEMBL
Match: A0A6J1HL09 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465515 PE=3 SV=1)

HSP 1 Score: 272 bits (695), Expect = 7.12e-87
Identity = 134/159 (84.28%), Postives = 147/159 (92.45%), Query Frame = 0

Query: 1   MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60
           MS+S L+ HRN+ FR ++LL LT++PNG +VGVNWGTMATHQLPPEKVVKMLQENGF KL
Sbjct: 1   MSKSGLQIHRNFTFRTILLLFLTIIPNGETVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120
           KLFEAEDRILEALIGTDIEVMLA+PNSMLQ+MS+D +AAA WVD+NVTAYTYHGGV IKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSDDSKAAASWVDANVTAYTYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 159
           VAVGNEPFLKSYNG YLQ TLPALRNIQQALNDAGLGSK
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSK 159

BLAST of Csor.00g085370 vs. ExPASy TrEMBL
Match: A0A6J1FB83 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111443850 PE=3 SV=1)

HSP 1 Score: 271 bits (693), Expect = 1.42e-86
Identity = 134/159 (84.28%), Postives = 146/159 (91.82%), Query Frame = 0

Query: 1   MSESVLRFHRNWIFRALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60
           MS+  L+ HRN+I R ++LL LT++PNG SVGVNWGTMATHQLPPEKVVKMLQENGF KL
Sbjct: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120
           KLFEAEDRILEALIGTDIEVMLA+PNSMLQ+MS+D +AAA WVD+NVTAYTYHGGV IKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSDDSKAAAYWVDANVTAYTYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSK 159
           VAVGNEPFLKSYNG YLQ TLPALRNIQQALNDAGLGSK
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSK 159

BLAST of Csor.00g085370 vs. TAIR 10
Match: AT3G24330.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 183.3 bits (464), Expect = 1.6e-46
Identity = 87/130 (66.92%), Postives = 107/130 (82.31%), Query Frame = 0

Query: 27  NGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALIGTDIEVMLAIPN 86
           N  +VGVNWG MA+HQLPPEKVVKML +N F KLKLFEA+  IL+ALIG+DIEVM+ IPN
Sbjct: 35  NTSNVGVNWGIMASHQLPPEKVVKMLMDNSFTKLKLFEADQNILDALIGSDIEVMIGIPN 94

Query: 87  SMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGIYLQSTLPALRN 146
             L+ M++D   AA WV+ NVTAY+Y+GGV IKY+AVGNEPFL++YNG Y++ TLPAL N
Sbjct: 95  RFLKEMAQDTSVAASWVEENVTAYSYNGGVNIKYIAVGNEPFLQTYNGTYVEFTLPALIN 154

Query: 147 IQQALNDAGL 157
           IQ+AL +A L
Sbjct: 155 IQRALEEADL 164

BLAST of Csor.00g085370 vs. TAIR 10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 160.2 bits (404), Expect = 1.4e-39
Identity = 77/128 (60.16%), Postives = 100/128 (78.12%), Query Frame = 0

Query: 31  VGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALIGTDIEVMLAIPNSMLQ 90
           +GVNWGTMATH+LPP+KVV+ML++N   K+KLF+A++  + AL G+ +EVM+AIPN  L+
Sbjct: 21  LGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETTMSALSGSGLEVMVAIPNDQLK 80

Query: 91  VMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGIYLQSTLPALRNIQQA 150
           VM    + A  WV  NVT Y ++GGV I +VAVGNEPFLKSYNG ++  T PAL+NIQ A
Sbjct: 81  VMG-SYDRAKDWVHKNVTRYNFNGGVNITFVAVGNEPFLKSYNGSFINLTFPALQNIQNA 140

Query: 151 LNDAGLGS 159
           LN+AGLGS
Sbjct: 141 LNEAGLGS 147

BLAST of Csor.00g085370 vs. TAIR 10
Match: AT1G64760.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 156.4 bits (394), Expect = 2.1e-38
Identity = 78/137 (56.93%), Postives = 103/137 (75.18%), Query Frame = 0

Query: 22  LTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALIGTDIEVM 81
           L ++ NG  +GVNWGTMATH+LPP+ VV+ML++N   K+KLF+A++  + AL G+ +EVM
Sbjct: 18  LGILVNG--LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVM 77

Query: 82  LAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGIYLQSTL 141
           +AIPN  L+VM+   + A  WV  NVT Y + GGV I +VAVGNEPFLKSYNG ++  T 
Sbjct: 78  VAIPNDQLKVMT-SYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTF 137

Query: 142 PALRNIQQALNDAGLGS 159
           PAL NIQ ALN+AGLG+
Sbjct: 138 PALANIQNALNEAGLGN 151

BLAST of Csor.00g085370 vs. TAIR 10
Match: AT1G64760.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 156.4 bits (394), Expect = 2.1e-38
Identity = 78/137 (56.93%), Postives = 103/137 (75.18%), Query Frame = 0

Query: 22  LTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALIGTDIEVM 81
           L ++ NG  +GVNWGTMATH+LPP+ VV+ML++N   K+KLF+A++  + AL G+ +EVM
Sbjct: 18  LGILVNG--LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVM 77

Query: 82  LAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGIYLQSTL 141
           +AIPN  L+VM+   + A  WV  NVT Y + GGV I +VAVGNEPFLKSYNG ++  T 
Sbjct: 78  VAIPNDQLKVMT-SYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTF 137

Query: 142 PALRNIQQALNDAGLGS 159
           PAL NIQ ALN+AGLG+
Sbjct: 138 PALANIQNALNEAGLGN 151

BLAST of Csor.00g085370 vs. TAIR 10
Match: AT3G04010.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 147.5 bits (371), Expect = 9.5e-36
Identity = 74/142 (52.11%), Postives = 104/142 (73.24%), Query Frame = 0

Query: 17  LILLLLTVVPNGFS-VGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALIG 76
           +I+  +  +  G S +GVNWGTMA+HQLPP+ VV+ML++N  +K+KLF+A+   + AL G
Sbjct: 18  IIISAVCFLSGGVSGLGVNWGTMASHQLPPKTVVEMLKDNNIQKVKLFDADTNTMGALAG 77

Query: 77  TDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGI 136
           + +EVM+AIPN +L  M  + + A  WV  NV+ + ++ GVKIKYVAVGNEPFL +YNG 
Sbjct: 78  SGVEVMVAIPNDLLLAMG-NYQRAKDWVQRNVSRFNFNNGVKIKYVAVGNEPFLTAYNGS 137

Query: 137 YLQSTLPALRNIQQALNDAGLG 158
           ++  T PAL NIQ ALN+AG+G
Sbjct: 138 FINLTYPALFNIQTALNEAGVG 158

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NKW92.9e-3756.93Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ... [more]
Q9M0882.5e-3348.98Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ... [more]
Q93Z083.3e-3351.03Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ... [more]
Q9FGH41.6e-2747.10Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ... [more]
A7PQW31.6e-1935.76Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120... [more]
Match NameE-valueIdentityDescription
KAG6599427.15.48e-115100.00Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023546836.12.30e-103100.00glucan endo-1,3-beta-glucosidase 8-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022946334.13.82e-10299.37glucan endo-1,3-beta-glucosidase 8-like isoform X1 [Cucurbita moschata][more]
XP_022999582.16.62e-10098.11glucan endo-1,3-beta-glucosidase 8-like isoform X1 [Cucurbita maxima][more]
KAG7030416.11.98e-9293.08Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. argyr... [more]
Match NameE-valueIdentityDescription
A0A6J1G3H91.85e-10299.37(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450439 P... [more]
A0A6J1KB853.21e-10098.11(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493909 PE=... [more]
A0A6J1DUS61.17e-8787.42(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111024222 ... [more]
A0A6J1HL097.12e-8784.28(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465515 PE=... [more]
A0A6J1FB831.42e-8684.28(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111443850 P... [more]
Match NameE-valueIdentityDescription
AT3G24330.11.6e-4666.92O-Glycosyl hydrolases family 17 protein [more]
AT2G19440.11.4e-3960.16O-Glycosyl hydrolases family 17 protein [more]
AT1G64760.12.1e-3856.93O-Glycosyl hydrolases family 17 protein [more]
AT1G64760.22.1e-3856.93O-Glycosyl hydrolases family 17 protein [more]
AT3G04010.19.5e-3652.11O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 29..165
e-value: 1.1E-39
score: 138.2
NoneNo IPR availablePANTHERPTHR32227:SF265GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 8-LIKEcoord: 13..158
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 31..159
e-value: 1.1E-19
score: 71.2
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 13..158
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 31..159

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g085370.m01Csor.00g085370.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds