Csor.00g084170 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g084170
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionbeta-fructofuranosidase, insoluble isoenzyme CWINV1-like
LocationCsor_Chr17: 7089164 .. 7091950 (-)
RNA-Seq ExpressionCsor.00g084170
SyntenyCsor.00g084170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCTCCTCTCTCTGCTTCATCTCTTTCCTCTGTTTCTTGCTCTGCCATGGAGCTTTGAAGCTGCAAGCTTCCCACCATGTTTACACACCTCAACCTTCTCGCCTTCTTCTTCATCATCATGAACAGCCTTACAGAACTTCCTGCCATTTTCAGCCTCCCAAGAACTGGATCAACGGTCTCTCTCTCTCTCTCTCTCTCTCTCGCTTTCTTTTCCATTTTTGTTCTTCTGGGTTTTTTTTCTTGTTCTTAAAGTTTCCATCTTTTTGTTTGTCATCTCAATTTGGCTATGGAACTGTCTCCCCAATCACAGATCCAAATGGTTTGTTCATCTCTCCTTGCTCTGTTGTTTTTATAAAACAAATTATTATTTGAATGATAATTGAACCGTTGTGATGATTTCAGGGCCAATGATATACAAGGGAATTTATCATCTGTTTTACCAATACAATCCTAAAGGAGCTGTGTGGGGTAACATTGTGTGGGCCCACTCCACATCAACGGACCTGATCAATTGGAAACCCCATGATTATGCCATCTACCCATCTCAGCCCTCCGATATCAACGGCTGCTGGTCGGGATCCGCCACCATTCTCCCTGGTGACAAACCGGCCATTCTGTACACAGGAATTAACCCTAAAAACCAACAAGTTCAAAACTTGGCTGTCCCTAAGAATCTGTCTGACCCATATCTTAGAGAATGGGTGAAATCCCCAAAGAATCCTCTCATGGCTCCCACCCCACAAAACCAAATCAACTCTAGCTCATTCCGCGACCCCACCACCGCCTGGCTCGGCCCCGATGGCGAATGGCGGGTCATCATCGGGAGCAAGATTGACGCTCGTGGACTTGCCATTATGTTCCGAAGCAAAGATTTTGTTAAGTGGGATCAGGTGCAGTAGAAAGCTAAAAACTCTATTGTCAATTTACGGTTGGTTTAAACTTGTAATAAAAGTTTTTGGTCCTTGATATCTAGGTTGATCATCCGCTTCATTATGCCGATGGCATGGGAATGTGGGAGTGTCCAGATTTCTTCCCTGTGGCAAAGAATGGTCGGGCGGGCGTTGACACGAGTGTGAGTGGACAACACGTGAAACACGTGTTGAAGGTGAGCTTGGATGATACTAAGCATGATCAGTACACGGTCGGAACATATGATTTGAAGAAAGATATTTATGTTCCGGATAAAGGATCGATCGAAGACTACAGGGGATTGAGATATGATTATGGAAAGTATTATGCTTCAAAGACATTCTTTGACGACCAAAAGAAGAGGAGAGTTTTGTGGGGTTGGGTGAATGAATCTTCAAGCGTCGATGATGATATCAAGAAGGGATGGTCGGGCGTCCAGGTAAGCAAGTCAAACTAAAATCTATCACTATGCATCATACTCAATACTACATTATCTCTGTCATCTCTCGAACTCCTTCTCCATTGAATATTTGGATCATTTCATGGACAACTTCAATGAAAATAAAACGGAGTTTAGTCATTCTCACCCTAAGTGGTGACCTGATTCAGCCTCGTCTTTTTTCTCCTATTCTTAGACCGCTGAAAGAAACCAAGGATGCAACCCATGTAGCTATTTATTTGTGACTTCTTTGACTGTAACCCACCCTATCCGCTAAAAATAAGAAAAAAAGAAAGTAAAATAATTAATTTTAAAAAGTTTTAAGACTAAAATAGATATTTCCCCCATAAACTTTATACCAAAATGATTATATTTATTTTATTGTTGCAGGCAATTCCAAGGACTGTTTGGCTTGATGCATCTGGAAAGCAACTAATTCAATGGCCAATTATGGAAATTCAAAAACTTCGAAACGACAAAGTTAAATTGGCCGACAAAGTATTGAAGAAAGGATCAATAATTGCAGTAAAAGGTGTCACTGCCGCACAGGTGAGTGACTGATTTTGTGTTAATTAAGGTTGTTTGATTTTGTGTTTGGTTTCTTATTGGCATTGTCATTATTATTACAGGCCGATGTTGATGTTTCATTCAAAGTGAAAAGCTTCAACAAAGCTCAGAAGCTGAAGGCTGAATGGAAAGACCCACAATTGGTTTGTAGCCAAATGGGAGCAGCTGCCAAGGGTGGAGTTGGTCCATTTGGGCTTCTAGTTTTGGCTTCCAACAACTTGGAAGAGTACACTTCGGTGTTCTTTAGGATATTCAAACGACAAGACAAGTATGTCGTGCTTATGTGTAGCGACCAAAGCAGGTATGCCGAACAACGTAATGCATAATTGTTTCATATCGTTCGATCGTGCATTGAACGATGAGAAAAGAAAAGTACCATTATTGAACCGTGACGACTTGACTTATTTGTTTATGTTTGAATTTTGCTTTTCTTGTTGGACAGGTCTTCGTTGAACGCAAATAATGACAAGACAACATATGGAGCATTCTTAGATGTAGACCCAGTTCGTAAAAACATTTCGTTGAGAAGTCTGGTGAGATTATTTTTTGAACAAATGGAACATTATCGTGTTTGAGTCTCGATATTCATAATTATTACTCTTCATGTACACGGGTTAAAGTCTATCAATTCTAACCATAGTAGATCTCGATCCATGAATTTGACCATTTTTATGAAACATTTACAGATTGATCACTCAATTGTCGAAAGCTTTGGATCTAAAGGAAAGGCTTGCATCACAGCGAGAGTGTATCCAAGGTTGGCGATTGGAGACAAAGCTGGTCTCTATGCTTTCAACAATGGAACAGAGAGTGTAACCATCACAAAACTCCGTGCTTGGAGCCTGAAAAAAGCTCAAATAATTTAA

mRNA sequence

ATGGCTTCCTCCTCTCTCTGCTTCATCTCTTTCCTCTGTTTCTTGCTCTGCCATGGAGCTTTGAAGCTGCAAGCTTCCCACCATGTTTACACACCTCAACCTTCTCGCCTTCTTCTTCATCATCATGAACAGCCTTACAGAACTTCCTGCCATTTTCAGCCTCCCAAGAACTGGATCAACGGGCCAATGATATACAAGGGAATTTATCATCTGTTTTACCAATACAATCCTAAAGGAGCTGTGTGGGGTAACATTGTGTGGGCCCACTCCACATCAACGGACCTGATCAATTGGAAACCCCATGATTATGCCATCTACCCATCTCAGCCCTCCGATATCAACGGCTGCTGGTCGGGATCCGCCACCATTCTCCCTGGTGACAAACCGGCCATTCTGTACACAGGAATTAACCCTAAAAACCAACAAGTTCAAAACTTGGCTGTCCCTAAGAATCTGTCTGACCCATATCTTAGAGAATGGGTGAAATCCCCAAAGAATCCTCTCATGGCTCCCACCCCACAAAACCAAATCAACTCTAGCTCATTCCGCGACCCCACCACCGCCTGGCTCGGCCCCGATGGCGAATGGCGGGTCATCATCGGGAGCAAGATTGACGCTCGTGGACTTGCCATTATGTTCCGAAGCAAAGATTTTGTTAAGTGGGATCAGGTTGATCATCCGCTTCATTATGCCGATGGCATGGGAATGTGGGAGTGTCCAGATTTCTTCCCTGTGGCAAAGAATGGTCGGGCGGGCGTTGACACGAGTGTGAGTGGACAACACGTGAAACACGTGTTGAAGGTGAGCTTGGATGATACTAAGCATGATCAGTACACGGTCGGAACATATGATTTGAAGAAAGATATTTATGTTCCGGATAAAGGATCGATCGAAGACTACAGGGGATTGAGATATGATTATGGAAAGTATTATGCTTCAAAGACATTCTTTGACGACCAAAAGAAGAGGAGAGTTTTGTGGGGTTGGGTGAATGAATCTTCAAGCGTCGATGATGATATCAAGAAGGGATGGTCGGGCGTCCAGGCAATTCCAAGGACTGTTTGGCTTGATGCATCTGGAAAGCAACTAATTCAATGGCCAATTATGGAAATTCAAAAACTTCGAAACGACAAAGTTAAATTGGCCGACAAAGTATTGAAGAAAGGATCAATAATTGCAGTAAAAGGTGTCACTGCCGCACAGGCCGATGTTGATGTTTCATTCAAAGTGAAAAGCTTCAACAAAGCTCAGAAGCTGAAGGCTGAATGGAAAGACCCACAATTGGTTTGTAGCCAAATGGGAGCAGCTGCCAAGGGTGGAGTTGGTCCATTTGGGCTTCTAGTTTTGGCTTCCAACAACTTGGAAGAGTACACTTCGGTGTTCTTTAGGATATTCAAACGACAAGACAAGTATGTCGTGCTTATGTGTAGCGACCAAAGCAGGTCTTCGTTGAACGCAAATAATGACAAGACAACATATGGAGCATTCTTAGATGTAGACCCAGTTCGTAAAAACATTTCGTTGAGAAGTCTGATTGATCACTCAATTGTCGAAAGCTTTGGATCTAAAGGAAAGGCTTGCATCACAGCGAGAGTGTATCCAAGGTTGGCGATTGGAGACAAAGCTGGTCTCTATGCTTTCAACAATGGAACAGAGAGTGTAACCATCACAAAACTCCGTGCTTGGAGCCTGAAAAAAGCTCAAATAATTTAA

Coding sequence (CDS)

ATGGCTTCCTCCTCTCTCTGCTTCATCTCTTTCCTCTGTTTCTTGCTCTGCCATGGAGCTTTGAAGCTGCAAGCTTCCCACCATGTTTACACACCTCAACCTTCTCGCCTTCTTCTTCATCATCATGAACAGCCTTACAGAACTTCCTGCCATTTTCAGCCTCCCAAGAACTGGATCAACGGGCCAATGATATACAAGGGAATTTATCATCTGTTTTACCAATACAATCCTAAAGGAGCTGTGTGGGGTAACATTGTGTGGGCCCACTCCACATCAACGGACCTGATCAATTGGAAACCCCATGATTATGCCATCTACCCATCTCAGCCCTCCGATATCAACGGCTGCTGGTCGGGATCCGCCACCATTCTCCCTGGTGACAAACCGGCCATTCTGTACACAGGAATTAACCCTAAAAACCAACAAGTTCAAAACTTGGCTGTCCCTAAGAATCTGTCTGACCCATATCTTAGAGAATGGGTGAAATCCCCAAAGAATCCTCTCATGGCTCCCACCCCACAAAACCAAATCAACTCTAGCTCATTCCGCGACCCCACCACCGCCTGGCTCGGCCCCGATGGCGAATGGCGGGTCATCATCGGGAGCAAGATTGACGCTCGTGGACTTGCCATTATGTTCCGAAGCAAAGATTTTGTTAAGTGGGATCAGGTTGATCATCCGCTTCATTATGCCGATGGCATGGGAATGTGGGAGTGTCCAGATTTCTTCCCTGTGGCAAAGAATGGTCGGGCGGGCGTTGACACGAGTGTGAGTGGACAACACGTGAAACACGTGTTGAAGGTGAGCTTGGATGATACTAAGCATGATCAGTACACGGTCGGAACATATGATTTGAAGAAAGATATTTATGTTCCGGATAAAGGATCGATCGAAGACTACAGGGGATTGAGATATGATTATGGAAAGTATTATGCTTCAAAGACATTCTTTGACGACCAAAAGAAGAGGAGAGTTTTGTGGGGTTGGGTGAATGAATCTTCAAGCGTCGATGATGATATCAAGAAGGGATGGTCGGGCGTCCAGGCAATTCCAAGGACTGTTTGGCTTGATGCATCTGGAAAGCAACTAATTCAATGGCCAATTATGGAAATTCAAAAACTTCGAAACGACAAAGTTAAATTGGCCGACAAAGTATTGAAGAAAGGATCAATAATTGCAGTAAAAGGTGTCACTGCCGCACAGGCCGATGTTGATGTTTCATTCAAAGTGAAAAGCTTCAACAAAGCTCAGAAGCTGAAGGCTGAATGGAAAGACCCACAATTGGTTTGTAGCCAAATGGGAGCAGCTGCCAAGGGTGGAGTTGGTCCATTTGGGCTTCTAGTTTTGGCTTCCAACAACTTGGAAGAGTACACTTCGGTGTTCTTTAGGATATTCAAACGACAAGACAAGTATGTCGTGCTTATGTGTAGCGACCAAAGCAGGTCTTCGTTGAACGCAAATAATGACAAGACAACATATGGAGCATTCTTAGATGTAGACCCAGTTCGTAAAAACATTTCGTTGAGAAGTCTGATTGATCACTCAATTGTCGAAAGCTTTGGATCTAAAGGAAAGGCTTGCATCACAGCGAGAGTGTATCCAAGGTTGGCGATTGGAGACAAAGCTGGTCTCTATGCTTTCAACAATGGAACAGAGAGTGTAACCATCACAAAACTCCGTGCTTGGAGCCTGAAAAAAGCTCAAATAATTTAA

Protein sequence

MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWINGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSSSFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMWECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSIEDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQKLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCSDQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLAIGDKAGLYAFNNGTESVTITKLRAWSLKKAQII
Homology
BLAST of Csor.00g084170 vs. ExPASy Swiss-Prot
Match: Q43866 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3702 GN=CWINV1 PE=1 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 1.3e-227
Identity = 383/570 (67.19%), Postives = 446/570 (78.25%), Query Frame = 0

Query: 12  LCFLLCHGALKLQASHHVY--TPQPSRLLLHHHEQPYRTSCHFQPPKNWI---NGPMIYK 71
           L  L+ +  + L+ASHHVY    Q +        QPYRT  HFQPPKNW+   NGPMIYK
Sbjct: 15  LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWMNDPNGPMIYK 74

Query: 72  GIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGSATILPG 131
           GIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH  AI+PS P DINGCWSGSATILP 
Sbjct: 75  GIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILPN 134

Query: 132 DKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSSSFRDPT 191
            KP ILYTGI+PKNQQVQN+A PKNLSDPYLREW KSP NPLMAP   N IN+SSFRDPT
Sbjct: 135 GKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDPT 194

Query: 192 TAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMWECPDFFPVA 251
           TAWLG D +WRVIIGSKI  RGLAI + SKDF+KW++   PLHY DG GMWECPDFFPV 
Sbjct: 195 TAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWECPDFFPVT 254

Query: 252 KNGRAGVDTSVSGQH---VKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSIEDYRGL 311
           + G  GV+TS  G+    +KHVLK+SLDDTKHD YT+GTYD  KD +VPD G   D    
Sbjct: 255 RFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGFKMDGTAP 314

Query: 312 RYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASGKQL 371
           RYDYGKYYASKTFFD  K RR+LWGW NESSSV+DD++KGWSG+Q IPR +WLD SGKQL
Sbjct: 315 RYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQL 374

Query: 372 IQWPIMEIQKLRNDKVK-LADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQKLKAE 431
           IQWP+ E+++LR  +VK L +KVLK GS + V GVTAAQADV+V FKV+   KA  ++  
Sbjct: 375 IQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPS 434

Query: 432 WKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFK---RQDKYVVLMCSDQ 491
           W DPQL+CS+M  + K G+GPFGL+VLAS NLEEYTSV+FRIFK     +KYVVLMCSDQ
Sbjct: 435 WTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQ 494

Query: 492 SRSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLAIG 551
           SRSSL  +NDKTTYGAF+D++P  + +SLR+LIDHS+VESFG KG+ACIT+RVYP+LAIG
Sbjct: 495 SRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACITSRVYPKLAIG 554

Query: 552 DKAGLYAFNNGTESVTITKLRAWSLKKAQI 570
             + L+AFN G +SV +  L AWS+  AQI
Sbjct: 555 KSSHLFAFNYGYQSVDVLNLNAWSMNSAQI 583

BLAST of Csor.00g084170 vs. ExPASy Swiss-Prot
Match: Q43089 (Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 PE=2 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 8.4e-214
Identity = 359/555 (64.68%), Postives = 430/555 (77.48%), Query Frame = 0

Query: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHH-----EQPYRTSCHFQPP 60
           MA SS+  +S    +     + ++A+HHVY  Q    L  HH      QPYRT+ HFQP 
Sbjct: 1   MAISSIFLLSLFSLIY---VIPIEATHHVY--QTLETLSSHHSSKSNHQPYRTAYHFQPL 60

Query: 61  KNWI---NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSD 120
           KNWI   NGPM Y G YHLFYQYNPKGAVWGNIVWAHS S DL+NW P D+AI+PSQPSD
Sbjct: 61  KNWINDPNGPMRYGGFYHLFYQYNPKGAVWGNIVWAHSVSKDLVNWTPLDHAIHPSQPSD 120

Query: 121 INGCWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 180
           I GCWSGSATILPG KPAILYTGI+P N QVQN+A+PKN+SDP LREW KSPKNPLM PT
Sbjct: 121 IKGCWSGSATILPGGKPAILYTGIDPNNHQVQNIAIPKNMSDPLLREWKKSPKNPLMEPT 180

Query: 181 PQNQINSSSFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYAD 240
             N+INSSSFRDPTT+WLG DG WRV+IGSKID +G+AI+++SK+FV W +  HPLH A+
Sbjct: 181 IANKINSSSFRDPTTSWLGKDGFWRVLIGSKIDTKGMAILYKSKNFVDWVEAKHPLHSAE 240

Query: 241 GMGMWECPDFFPVAKNG--RAGVDTSVSG-QHVKHVLKVSLDDTKHDQYTVGTYDLKKDI 300
           G GMWECPDF+PV      R GVDTS +G   V+HVLKVSLDDTKHD Y +G+YD+ KD+
Sbjct: 241 GTGMWECPDFYPVLDKNLLRTGVDTSRNGDDDVRHVLKVSLDDTKHDHYLIGSYDVVKDV 300

Query: 301 YVPDKGSIEDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQA 360
           +VP+ G  ++   LRYDYGKYYASKTFFDD K RR+L GWVNESSSV DD+KKGWSG+  
Sbjct: 301 FVPENGFEDNGFVLRYDYGKYYASKTFFDDGKNRRILLGWVNESSSVADDVKKGWSGIHT 360

Query: 361 IPRTVWLDASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFK 420
           IPR +WL  SGKQL+QWP+ EI+ LR + V    KV+K G  I++ GV + QADV++SF+
Sbjct: 361 IPREIWLHESGKQLVQWPVKEIENLRMNPVNWPTKVIKGGERISITGVDSVQADVEISFE 420

Query: 421 VKSFNKAQKLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQD 480
           +    K + L+ +W DPQL+CSQ GA  KGGVGPFGLLV AS  L+EYT+VFFRIFK QD
Sbjct: 421 ISDLGKVESLR-KWIDPQLLCSQKGAGVKGGVGPFGLLVFASQGLKEYTAVFFRIFKYQD 480

Query: 481 KYVVLMCSDQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACIT 540
           K +VLMCSDQSRSSLN +ND T+YG F+DVDP+ + +SLR+LIDHS+VESFG +G+AC+T
Sbjct: 481 KNLVLMCSDQSRSSLNKDNDMTSYGTFVDVDPLHEKLSLRTLIDHSVVESFGGEGRACVT 540

Query: 541 ARVYPRLAIGDKAGL 545
           ARVYP LAI DKA L
Sbjct: 541 ARVYPTLAIHDKAML 549

BLAST of Csor.00g084170 vs. ExPASy Swiss-Prot
Match: Q70XE6 (Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 5.5e-213
Identity = 362/580 (62.41%), Postives = 437/580 (75.34%), Query Frame = 0

Query: 3   SSSLCFISFLCFLLCHGAL---KLQASHHVYTPQPSRLLLHHH----EQPYRTSCHFQPP 62
           + S   +S    LL HG +   K QA HH        L+  H     + PYRT+ HFQ P
Sbjct: 5   NGSWLVLSISMMLLSHGMIIIAKDQAIHHHDDDHDDMLINDHQMINDDDPYRTAYHFQSP 64

Query: 63  KNWI---NGPMIYKGIYHLFYQYNPKGAVW-GNIVWAHSTSTDLINWKPHDYAIYPSQPS 122
           KNW+   NGPMIYKGIYHLFYQY P   VW   IVW HSTSTDLINW     A+ PS+P 
Sbjct: 65  KNWMNDPNGPMIYKGIYHLFYQYYPYDPVWHTEIVWGHSTSTDLINWTQQPIALSPSEPY 124

Query: 123 DINGCWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMA- 182
           DINGCWSGS TILP +KP ILYTGIN KN QVQNLA+PKNLSDPYL+EW+K P+NPLMA 
Sbjct: 125 DINGCWSGSITILPQNKPVILYTGINNKNYQVQNLALPKNLSDPYLKEWIKLPQNPLMAG 184

Query: 183 -PTPQNQINSSSFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLH 242
            PT  N IN+SSFRDP+TAW   DG+WRVI+G++   RGLA++F S DFVKW+   +PLH
Sbjct: 185 TPTNNNNINASSFRDPSTAWQLSDGKWRVIVGTQQGKRGLAVLFTSDDFVKWNNTGNPLH 244

Query: 243 YADGMGMWECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDI 302
             +G G+WECPDFFPV      G DTS+ G  VKHVLK+SL DT+++ YT+G YD++KDI
Sbjct: 245 STEGNGIWECPDFFPVYVGKSLGADTSIIGDDVKHVLKLSLFDTQYEYYTIGRYDIEKDI 304

Query: 303 YVPDKGSIEDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQA 362
           YVPD+GSIE   GLRYDYGK+YASK+FFDD+  RR+LWGWVNESS   DDIKKGWSGVQA
Sbjct: 305 YVPDEGSIESDLGLRYDYGKFYASKSFFDDETNRRILWGWVNESSIQADDIKKGWSGVQA 364

Query: 363 IPRTVWLDASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFK 422
           IPRTV LD SGKQL+QWP+ E+  LR + V+L  +V+K GS++ +  +TA+QADV++SFK
Sbjct: 365 IPRTVVLDKSGKQLVQWPLAEVDMLRENDVELPSQVIKGGSLVEISQITASQADVEISFK 424

Query: 423 VKSFNKAQKLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQD 482
           +   N  ++L +   +PQ++CSQ GA+ KG  GPFGLL LAS  LEEYT+VFFRIFK  +
Sbjct: 425 IPESNYVEELDSTCTNPQILCSQKGASIKGRFGPFGLLTLASMGLEEYTAVFFRIFKGPN 484

Query: 483 KYVVLMCSDQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACIT 542
           KYVVLMCSDQ+RSSLN   DK ++G F+DVDP+ +++SLR LIDHSIVESF +KGK+CIT
Sbjct: 485 KYVVLMCSDQTRSSLNPTTDKLSFGIFVDVDPINEDLSLRILIDHSIVESFSAKGKSCIT 544

Query: 543 ARVYPRLAIGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 570
           ARVYP +AI DKA LY FNNGTE V ITKL AWS+KKAQI
Sbjct: 545 ARVYPTMAINDKAKLYVFNNGTEDVKITKLSAWSMKKAQI 584

BLAST of Csor.00g084170 vs. ExPASy Swiss-Prot
Match: Q67XZ3 (Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3702 GN=CWINV3 PE=1 SV=2)

HSP 1 Score: 723.8 bits (1867), Expect = 1.5e-207
Identity = 349/538 (64.87%), Postives = 407/538 (75.65%), Query Frame = 0

Query: 44  QPYRTSCHFQPPKNWI---NGPMIYKGIYHLFYQYNPKGAVWG-NIVWAHSTSTDLINWK 103
           QPYRT  HFQP KNW+   NGPMIYKGIYHLFYQYNP GAVW   IVW HSTS DL+NW 
Sbjct: 36  QPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWI 95

Query: 104 PHDYAIYPSQPSDINGCWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLRE 163
               A  PSQPSDINGCWSGS TILP  KP ILYTGI+    QVQN+AVP N+SDPYLRE
Sbjct: 96  SQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLRE 155

Query: 164 WVKSPKNPLMAPTPQNQINSSSFRDPTTAWLGPDGEWRVIIGSKI-DARGLAIMFRSKDF 223
           W K P+NPLM     N IN   FRDPTTAWLG DGEWRVI+GS   D RGLAI+++S+DF
Sbjct: 156 WSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDF 215

Query: 224 VKWDQVDHPLHYADGMGMWECPDFFPVAKNGRAGVDTSVSGQH-VKHVLKVSLDDTKHDQ 283
             W Q   PLHY D  GMWECPDFFPV+  G  GV+TS  G++ +KHVLKVSL +T HD 
Sbjct: 216 FNWTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDY 275

Query: 284 YTVGTYDLKKDIYVPDKGSIEDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVD 343
           YT+G+YD +KD+YVPD G +++    R DYGKYYASKTF+DD KKRR+LWGWVNESS   
Sbjct: 276 YTIGSYDREKDVYVPDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAK 335

Query: 344 DDIKKGWSGVQAIPRTVWLDASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGV 403
           DDI+KGWSG+Q+ PR +WLD SGK+L+QWPI EI+ LR  +V    KVLK GS + V GV
Sbjct: 336 DDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGV 395

Query: 404 TAAQADVDVSFKVKSFNKAQKLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEY 463
           TAAQADV+VSFKVK   KA  ++  W DPQ +CSQ   +   G+GPFGL+VLASN++EEY
Sbjct: 396 TAAQADVEVSFKVKELEKADVIEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEY 455

Query: 464 TSVFFRIFKRQD------KYVVLMCSDQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSL 523
           TSV+FRIFK  D      KYVVLMCSDQSRSSLN  NDK+T+GAF+ +DP  + ISLR+L
Sbjct: 456 TSVYFRIFKSNDDTNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTL 515

Query: 524 IDHSIVESFGSKGKACITARVYPRLAIGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 570
           IDHSIVES+G  G+ CIT+RVYP+LAIG+ A L+ FN GT+SV I  L AWSLK AQI
Sbjct: 516 IDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQI 573

BLAST of Csor.00g084170 vs. ExPASy Swiss-Prot
Match: Q9LIB9 (Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3702 GN=CWINV5 PE=2 SV=2)

HSP 1 Score: 699.5 bits (1804), Expect = 3.1e-200
Identity = 340/535 (63.55%), Postives = 407/535 (76.07%), Query Frame = 0

Query: 44  QPYRTSCHFQPPKNWI---NGPMIYKGIYHLFYQYNPKGAVW--GNIVWAHSTSTDLINW 103
           QPYRT  HFQPPKNW+   NGPMIYKGIYHLFYQ+N  GAV      VW H+TSTDLINW
Sbjct: 40  QPYRTGYHFQPPKNWMNDPNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTDLINW 99

Query: 104 KPHDYAIYPSQPSDINGCWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLR 163
                AI PS+PSDINGCWSGS TILP  KP ILYTG +  N+QVQNL  PKNL+DPYLR
Sbjct: 100 ITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTDPYLR 159

Query: 164 EWVKSPKNPLMAPTPQNQINSSSFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDF 223
            W KSP+NPL+ P+P N INSS+FRDPTTAW G DG WR+  GS+   RGLAI+  SKDF
Sbjct: 160 HWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSKDF 219

Query: 224 VKWDQVDHPLHYADGMGMWECPDFFPVAKNGRAGVDTSV-SGQHVKHVLKVSLDDTKHDQ 283
           V W Q   PLHY DG GMWECPDFFPVA+    G+DTS  SG  VKHVLKVSL DT +D 
Sbjct: 220 VIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDTFNDY 279

Query: 284 YTVGTYDLKKDIYVPDKGSIEDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVD 343
           YT+GTYD  +D+YVPDKG ++D    RYDYGK+YASKTF+D   +RR+LWGWVNESS   
Sbjct: 280 YTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNESSPEK 339

Query: 344 DDIKKGWSGVQAIPRTVWLDASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGV 403
           D+IKKGW+G+QAIPR VWLD SGK+L+QWP+ EI++LR  +VK  +K+LK GS++ V GV
Sbjct: 340 DNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVMEVHGV 399

Query: 404 TAAQADVDVSFKVKSFN--KAQKLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLE 463
           TA QADV+V FKV  F+  KA  ++  W DPQL+CSQ  A++  G+GPFGL+VLAS N+E
Sbjct: 400 TAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRNASS--GLGPFGLMVLASKNME 459

Query: 464 EYTSVFFRIFK---RQDKYVVLMCSDQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSLI 523
           EYTSV  RIF+      ++VV+MCSDQS SSL   NDKTTYGAFLD+ P +  ISLR+LI
Sbjct: 460 EYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISPYQP-ISLRTLI 519

Query: 524 DHSIVESFGSKGKACITARVYPRLAIGDKAGLYAFNNGTESVTITKLRAWSLKKA 568
           D SIVESFG KGK CIT+RVYP+LAIG++  L+AFN G+++V +  L AWS+K +
Sbjct: 520 DKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMKSS 571

BLAST of Csor.00g084170 vs. NCBI nr
Match: KAG6575468.1 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1179 bits (3051), Expect = 0.0
Identity = 570/570 (100.00%), Postives = 570/570 (100.00%), Query Frame = 0

Query: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60
           MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60

Query: 61  GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGS 120
           GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGS
Sbjct: 61  GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGS 120

Query: 121 ATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSS 180
           ATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSS
Sbjct: 121 ATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSS 180

Query: 181 SFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMWECP 240
           SFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMWECP
Sbjct: 181 SFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMWECP 240

Query: 241 DFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSIEDY 300
           DFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSIEDY
Sbjct: 241 DFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSIEDY 300

Query: 301 RGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASG 360
           RGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASG
Sbjct: 301 RGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASG 360

Query: 361 KQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQKLK 420
           KQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQKLK
Sbjct: 361 KQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQKLK 420

Query: 421 AEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCSDQS 480
           AEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCSDQS
Sbjct: 421 AEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCSDQS 480

Query: 481 RSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLAIGD 540
           RSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLAIGD
Sbjct: 481 RSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLAIGD 540

Query: 541 KAGLYAFNNGTESVTITKLRAWSLKKAQII 570
           KAGLYAFNNGTESVTITKLRAWSLKKAQII
Sbjct: 541 KAGLYAFNNGTESVTITKLRAWSLKKAQII 570

BLAST of Csor.00g084170 vs. NCBI nr
Match: XP_022954013.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata])

HSP 1 Score: 1144 bits (2959), Expect = 0.0
Identity = 553/573 (96.51%), Postives = 560/573 (97.73%), Query Frame = 0

Query: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60
           MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60

Query: 61  ---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120
              GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQI 180
           SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN I
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLGPDGEWRVIIGSK+D RGLAIMFRSKDFVKWDQVDHPLHYADGMGMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKVDDRGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240

Query: 241 ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300
           ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 EDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           +DY GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD
Sbjct: 301 QDYSGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360

Query: 361 ASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420
           ASGKQLIQWPI EIQKLR DK+KL +KVL+KGSIIAVKGVTAAQADVDVSFKVKSFNKA 
Sbjct: 361 ASGKQLIQWPIAEIQKLRKDKIKLTNKVLEKGSIIAVKGVTAAQADVDVSFKVKSFNKAH 420

Query: 421 KLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480
            LKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS
Sbjct: 421 LLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480

Query: 481 DQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLA 540
           DQSRSSLNA+NDKT YGAFLDVDPVRKN+SLRSLIDHSIVESFGSKGKACITARVYPRLA
Sbjct: 481 DQSRSSLNADNDKTMYGAFLDVDPVRKNVSLRSLIDHSIVESFGSKGKACITARVYPRLA 540

Query: 541 IGDKAGLYAFNNGTESVTITKLRAWSLKKAQII 570
           IGDKAGLYAFNNGTESVTITKLRAWSLKKAQII
Sbjct: 541 IGDKAGLYAFNNGTESVTITKLRAWSLKKAQII 573

BLAST of Csor.00g084170 vs. NCBI nr
Match: XP_023547447.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1129 bits (2921), Expect = 0.0
Identity = 549/573 (95.81%), Postives = 555/573 (96.86%), Query Frame = 0

Query: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60
           MASSSLCFISFLCFLL HGALKLQASHHVYT Q SRLLLHHHEQPYRTS HFQPPKNWIN
Sbjct: 1   MASSSLCFISFLCFLLAHGALKLQASHHVYTAQASRLLLHHHEQPYRTSYHFQPPKNWIN 60

Query: 61  ---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120
              GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQI 180
           SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN I
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG DGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240

Query: 241 ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300
           ECPDFFPVAKNG AGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNGHAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 EDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           EDY GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR VWLD
Sbjct: 301 EDYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360

Query: 361 ASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420
           ASGKQLIQWPI EIQKLR DK+KL +KVLKKGSI+AVKGVTAAQADVDVSFKVKSFNKAQ
Sbjct: 361 ASGKQLIQWPIAEIQKLRKDKIKLTNKVLKKGSIVAVKGVTAAQADVDVSFKVKSFNKAQ 420

Query: 421 KLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480
           +LKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFK QDKYVVLMCS
Sbjct: 421 RLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKGQDKYVVLMCS 480

Query: 481 DQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLA 540
           DQSRSSLNANNDKTTYGAFLDVDPVR+N+SLRSLIDHSIVESFGSKGKACITARVYPRLA
Sbjct: 481 DQSRSSLNANNDKTTYGAFLDVDPVRENVSLRSLIDHSIVESFGSKGKACITARVYPRLA 540

Query: 541 IGDKAGLYAFNNGTESVTITKLRAWSLKKAQII 570
           IGDKAGLYAFNNGTESVTI KLRAWSLKKAQII
Sbjct: 541 IGDKAGLYAFNNGTESVTIAKLRAWSLKKAQII 573

BLAST of Csor.00g084170 vs. NCBI nr
Match: XP_022954011.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata] >XP_022954012.1 beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata])

HSP 1 Score: 1127 bits (2916), Expect = 0.0
Identity = 549/572 (95.98%), Postives = 555/572 (97.03%), Query Frame = 0

Query: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60
           MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLH +EQPYRTS HFQPPKNWIN
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  ---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120
              GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQI 180
           SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN I
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG DGEWRVIIGSKIDARGLAIMF+SKDFVKWDQVDHPLHYADG GMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300
           ECPDFFPVAKN RAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNSRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 EDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E Y GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR VWLD
Sbjct: 301 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360

Query: 361 ASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420
           ASGKQLIQWPI+EIQKLR DKVKL +KVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ
Sbjct: 361 ASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420

Query: 421 KLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480
            LKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS
Sbjct: 421 PLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480

Query: 481 DQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLA 540
           DQSRSSLNANNDKTTYGAFLDVDPVR+NISLRSLIDHSIVESFGSKGKACITARVYPRLA
Sbjct: 481 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPRLA 540

Query: 541 IGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 569
           IGDKAGLYAFNNGTESVTITKL AWS+KKAQI
Sbjct: 541 IGDKAGLYAFNNGTESVTITKLSAWSMKKAQI 572

BLAST of Csor.00g084170 vs. NCBI nr
Match: KAG6575469.1 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1127 bits (2914), Expect = 0.0
Identity = 546/569 (95.96%), Postives = 553/569 (97.19%), Query Frame = 0

Query: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60
           MASSSLCFISFLCFLLCHGALKLQASHHVY  Q SRLLLH +EQPYRTS HFQPPKNWIN
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYRAQASRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGS 120
           GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGS
Sbjct: 61  GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGS 120

Query: 121 ATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSS 180
           ATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSS
Sbjct: 121 ATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSS 180

Query: 181 SFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMWECP 240
           SFRDPTTAWLGPD EWRVIIGSKIDARGLAIMF+SKDFVKWDQVDHPLHYADG GMWECP
Sbjct: 181 SFRDPTTAWLGPDSEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMWECP 240

Query: 241 DFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSIEDY 300
           DFFPVAKNGRAGVDTSVSG+HVKHVLKVSLDDTKHD YTVGTYDLKKDIYVPDKGSIE Y
Sbjct: 241 DFFPVAKNGRAGVDTSVSGRHVKHVLKVSLDDTKHDHYTVGTYDLKKDIYVPDKGSIEGY 300

Query: 301 RGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASG 360
            GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR VWLDASG
Sbjct: 301 TGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLDASG 360

Query: 361 KQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQKLK 420
           KQLIQWPI+EIQKLR DKVKL +KVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ LK
Sbjct: 361 KQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQPLK 420

Query: 421 AEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCSDQS 480
           AEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCSDQS
Sbjct: 421 AEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCSDQS 480

Query: 481 RSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLAIGD 540
           RSSLNANNDKTTYGAFLDVDPVR+NISLRSLIDHSIVESFGSKGKACITARVYPRLAIGD
Sbjct: 481 RSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPRLAIGD 540

Query: 541 KAGLYAFNNGTESVTITKLRAWSLKKAQI 569
           KAGLYAFNNGTESVTITKL AWS+KKAQI
Sbjct: 541 KAGLYAFNNGTESVTITKLSAWSMKKAQI 569

BLAST of Csor.00g084170 vs. ExPASy TrEMBL
Match: A0A6J1GPY1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456400 PE=3 SV=1)

HSP 1 Score: 1144 bits (2959), Expect = 0.0
Identity = 553/573 (96.51%), Postives = 560/573 (97.73%), Query Frame = 0

Query: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60
           MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60

Query: 61  ---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120
              GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQI 180
           SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN I
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLGPDGEWRVIIGSK+D RGLAIMFRSKDFVKWDQVDHPLHYADGMGMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKVDDRGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240

Query: 241 ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300
           ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 EDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           +DY GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD
Sbjct: 301 QDYSGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360

Query: 361 ASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420
           ASGKQLIQWPI EIQKLR DK+KL +KVL+KGSIIAVKGVTAAQADVDVSFKVKSFNKA 
Sbjct: 361 ASGKQLIQWPIAEIQKLRKDKIKLTNKVLEKGSIIAVKGVTAAQADVDVSFKVKSFNKAH 420

Query: 421 KLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480
            LKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS
Sbjct: 421 LLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480

Query: 481 DQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLA 540
           DQSRSSLNA+NDKT YGAFLDVDPVRKN+SLRSLIDHSIVESFGSKGKACITARVYPRLA
Sbjct: 481 DQSRSSLNADNDKTMYGAFLDVDPVRKNVSLRSLIDHSIVESFGSKGKACITARVYPRLA 540

Query: 541 IGDKAGLYAFNNGTESVTITKLRAWSLKKAQII 570
           IGDKAGLYAFNNGTESVTITKLRAWSLKKAQII
Sbjct: 541 IGDKAGLYAFNNGTESVTITKLRAWSLKKAQII 573

BLAST of Csor.00g084170 vs. ExPASy TrEMBL
Match: A0A6J1GPU7 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456399 PE=3 SV=1)

HSP 1 Score: 1127 bits (2916), Expect = 0.0
Identity = 549/572 (95.98%), Postives = 555/572 (97.03%), Query Frame = 0

Query: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60
           MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLH +EQPYRTS HFQPPKNWIN
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  ---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120
              GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQI 180
           SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN I
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG DGEWRVIIGSKIDARGLAIMF+SKDFVKWDQVDHPLHYADG GMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300
           ECPDFFPVAKN RAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNSRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 EDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E Y GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR VWLD
Sbjct: 301 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360

Query: 361 ASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420
           ASGKQLIQWPI+EIQKLR DKVKL +KVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ
Sbjct: 361 ASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420

Query: 421 KLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480
            LKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS
Sbjct: 421 PLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480

Query: 481 DQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLA 540
           DQSRSSLNANNDKTTYGAFLDVDPVR+NISLRSLIDHSIVESFGSKGKACITARVYPRLA
Sbjct: 481 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPRLA 540

Query: 541 IGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 569
           IGDKAGLYAFNNGTESVTITKL AWS+KKAQI
Sbjct: 541 IGDKAGLYAFNNGTESVTITKLSAWSMKKAQI 572

BLAST of Csor.00g084170 vs. ExPASy TrEMBL
Match: A0A6J1JP62 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita maxima OX=3661 GN=LOC111488605 PE=3 SV=1)

HSP 1 Score: 1081 bits (2796), Expect = 0.0
Identity = 521/572 (91.08%), Postives = 543/572 (94.93%), Query Frame = 0

Query: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60
           MASSSLCFIS LCFLLCHGALKLQASHHVYT Q SRLLLHH+EQPYRTS HFQPPKNWIN
Sbjct: 3   MASSSLCFISLLCFLLCHGALKLQASHHVYTAQASRLLLHHYEQPYRTSYHFQPPKNWIN 62

Query: 61  ---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120
              GPMIYKGIYHLFYQYNPKGA WGNIVWAHSTSTDLINWKPHD+AIYPSQPSDINGCW
Sbjct: 63  DPNGPMIYKGIYHLFYQYNPKGATWGNIVWAHSTSTDLINWKPHDHAIYPSQPSDINGCW 122

Query: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQI 180
           SGSATILPG+KPAILYTGINPKNQQVQNLA+PKNLSDPYLREWVKSP NPLMAPTPQN+I
Sbjct: 123 SGSATILPGEKPAILYTGINPKNQQVQNLALPKNLSDPYLREWVKSPNNPLMAPTPQNRI 182

Query: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG DG+WRVIIGSKIDARGLAIMFRSKDFV+WDQVDHPLHYADG GMW
Sbjct: 183 NSSSFRDPTTAWLGHDGQWRVIIGSKIDARGLAIMFRSKDFVRWDQVDHPLHYADGTGMW 242

Query: 241 ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300
           ECPDFFPVAKNGRAG+DTSV+G HVKHVLKVSLDDTKHD YTVGTYDLKKDIYVPDK S 
Sbjct: 243 ECPDFFPVAKNGRAGIDTSVNGGHVKHVLKVSLDDTKHDHYTVGTYDLKKDIYVPDKEST 302

Query: 301 EDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E Y GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDD+KKGWSGVQAIPR VWLD
Sbjct: 303 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDMKKGWSGVQAIPRAVWLD 362

Query: 361 ASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420
           ASGKQLIQWPI EIQKLR DK+KL +KV+KKGSIIAVKGVTAAQADV+VSFKVKSF KAQ
Sbjct: 363 ASGKQLIQWPIAEIQKLRKDKIKLRNKVVKKGSIIAVKGVTAAQADVEVSFKVKSFKKAQ 422

Query: 421 KLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480
           +LKAEWKDPQL CS+MGAAA GGVGPFGLLVLAS +LEEYTSVFFR+FK QDKYVVLMCS
Sbjct: 423 RLKAEWKDPQLACSRMGAAAMGGVGPFGLLVLASKDLEEYTSVFFRVFKGQDKYVVLMCS 482

Query: 481 DQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLA 540
           DQSRSSLNANNDKTTYGAFLDVDPVR+NISLRSLIDHSIVESFGSKGKACITARVYP+LA
Sbjct: 483 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPKLA 542

Query: 541 IGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 569
           +GDKAGLYAFNNGTE VTITKL AWS+KKAQI
Sbjct: 543 MGDKAGLYAFNNGTERVTITKLSAWSMKKAQI 574

BLAST of Csor.00g084170 vs. ExPASy TrEMBL
Match: A0A0A0K761 (Cell wall invertase 5 OS=Cucumis sativus OX=3659 GN=Csa_7G450770 PE=2 SV=1)

HSP 1 Score: 977 bits (2526), Expect = 0.0
Identity = 470/577 (81.46%), Postives = 518/577 (89.77%), Query Frame = 0

Query: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHH--HEQPYRTSCHFQPPKNW 60
           MAS SLCFISFLCFL  HG  +LQASHHV+ PQ S  L +H  H QPYRTS HFQPPKNW
Sbjct: 1   MASISLCFISFLCFLFGHGVFQLQASHHVHKPQTSTSLPYHYHHRQPYRTSYHFQPPKNW 60

Query: 61  IN---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDING 120
           IN   GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW+PHD+AI+PSQPSDING
Sbjct: 61  INDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSDING 120

Query: 121 CWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN 180
           CWSGSATILPG+KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN
Sbjct: 121 CWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN 180

Query: 181 QINSSSFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMG 240
           QIN+SSFRDPTTAWLGP+GEWRVI+G+K+  RGLA+MF+SKDF+KWDQVDHPLHYADG G
Sbjct: 181 QINASSFRDPTTAWLGPNGEWRVIVGNKVHTRGLALMFQSKDFIKWDQVDHPLHYADGSG 240

Query: 241 MWECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKG 300
           MWECPDF+PVAK GR GVDT+V+G++VKHVLKVSLDDT+HD YT+GTYD++KDIYVP+KG
Sbjct: 241 MWECPDFYPVAKTGRRGVDTTVNGKNVKHVLKVSLDDTRHDVYTIGTYDVEKDIYVPNKG 300

Query: 301 SIEDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVW 360
           SIE Y GLRYDYGKYYASKTF+D QKKRRVLWGWVNESSSV+DDIKKGWSG+Q IPRT+W
Sbjct: 301 SIEGYSGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQGIPRTIW 360

Query: 361 LDASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNK 420
           LDASGKQLIQWPI EIQKLR +KV L +KVLKKGS I VKGVT +QADV+VSFKVK+  K
Sbjct: 361 LDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSTIEVKGVTPSQADVEVSFKVKNLKK 420

Query: 421 AQKLKAE-WKDPQLVCSQMGAAA--KGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYV 480
           AQ LK + W++PQ++CSQ G AA  KGGVGPFGLLVLASN+ +EYTSV F IFK+Q KYV
Sbjct: 421 AQILKPQYWENPQVLCSQKGRAATTKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQSKYV 480

Query: 481 VLMCSDQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARV 540
            LMCSDQSRSSLN  NDKTTYGAFLDVD   +++SLRSLIDHS+VESFG KGK CITARV
Sbjct: 481 ALMCSDQSRSSLNPTNDKTTYGAFLDVDVDSEDLSLRSLIDHSVVESFGLKGKGCITARV 540

Query: 541 YPRLAIGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 569
           YP LAIGD  GLYAFNNGTESVTITKL AWS+KKA+I
Sbjct: 541 YPTLAIGDNVGLYAFNNGTESVTITKLTAWSMKKARI 577

BLAST of Csor.00g084170 vs. ExPASy TrEMBL
Match: A0A6J1DM23 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Momordica charantia OX=3673 GN=LOC111021302 PE=3 SV=1)

HSP 1 Score: 973 bits (2515), Expect = 0.0
Identity = 464/572 (81.12%), Postives = 512/572 (89.51%), Query Frame = 0

Query: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60
           MAS SL F SFL   LCHG  KL+ASHHVY  Q S  +   H+QPYRTS HFQPPKNW+N
Sbjct: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60

Query: 61  ---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120
              GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH +AI+PSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120

Query: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQI 180
           SGSATILPG+KPA+LYTGINP+N QVQNLAVPKNLSDP+L EWVKSPKNPLMAPTPQN I
Sbjct: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180

Query: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG D +WRV+IGSKI+ARGLAIM+RSKDF++WDQV++PLHYAD  GMW
Sbjct: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240

Query: 241 ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300
           ECPDFFPVAKN R GVDTSV+G  VKHVLKVSLDDTKHD YTVGTY++KKD+YVPDKGS+
Sbjct: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300

Query: 301 EDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E Y GLRYDYGK YASKTFFD +KKRRVLWGWVNESSSVDDD KKGWSGVQAIPR++WLD
Sbjct: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360

Query: 361 ASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420
           ASGKQL+QWPI+EIQKLR DKVKL +KVLKKGS+I VKGVTAAQ DV+VSFKVK+F KAQ
Sbjct: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420

Query: 421 KLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480
            LK EWKDPQL+C+Q GAA + GVGPFGLL+LAS +L+EYTSVFF++FKR+ KYVVLMCS
Sbjct: 421 VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS 480

Query: 481 DQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLA 540
           DQSRSSLN+NNDKTTYGAF+D+DPVR+N+SLRSLIDHSIVESFG KGKACITARVYP LA
Sbjct: 481 DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLA 540

Query: 541 IGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 569
           IG+  GLYAFNN TE VTI+KL AWSL KA I
Sbjct: 541 IGENVGLYAFNNATEGVTISKLTAWSLNKAHI 572

BLAST of Csor.00g084170 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 795.8 bits (2054), Expect = 2.3e-230
Identity = 383/567 (67.55%), Postives = 446/567 (78.66%), Query Frame = 0

Query: 12  LCFLLCHGALKLQASHHVY--TPQPSRLLLHHHEQPYRTSCHFQPPKNWINGPMIYKGIY 71
           L  L+ +  + L+ASHHVY    Q +        QPYRT  HFQPPKNW+NGPMIYKGIY
Sbjct: 15  LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWMNGPMIYKGIY 74

Query: 72  HLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGSATILPGDKP 131
           HLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH  AI+PS P DINGCWSGSATILP  KP
Sbjct: 75  HLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILPNGKP 134

Query: 132 AILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSSSFRDPTTAW 191
            ILYTGI+PKNQQVQN+A PKNLSDPYLREW KSP NPLMAP   N IN+SSFRDPTTAW
Sbjct: 135 VILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDPTTAW 194

Query: 192 LGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMWECPDFFPVAKNG 251
           LG D +WRVIIGSKI  RGLAI + SKDF+KW++   PLHY DG GMWECPDFFPV + G
Sbjct: 195 LGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWECPDFFPVTRFG 254

Query: 252 RAGVDTSVSGQH---VKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSIEDYRGLRYD 311
             GV+TS  G+    +KHVLK+SLDDTKHD YT+GTYD  KD +VPD G   D    RYD
Sbjct: 255 SNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGFKMDGTAPRYD 314

Query: 312 YGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQW 371
           YGKYYASKTFFD  K RR+LWGW NESSSV+DD++KGWSG+Q IPR +WLD SGKQLIQW
Sbjct: 315 YGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQLIQW 374

Query: 372 PIMEIQKLRNDKVK-LADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQKLKAEWKD 431
           P+ E+++LR  +VK L +KVLK GS + V GVTAAQADV+V FKV+   KA  ++  W D
Sbjct: 375 PVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPSWTD 434

Query: 432 PQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFK---RQDKYVVLMCSDQSRS 491
           PQL+CS+M  + K G+GPFGL+VLAS NLEEYTSV+FRIFK     +KYVVLMCSDQSRS
Sbjct: 435 PQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQSRS 494

Query: 492 SLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLAIGDKA 551
           SL  +NDKTTYGAF+D++P  + +SLR+LIDHS+VESFG KG+ACIT+RVYP+LAIG  +
Sbjct: 495 SLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACITSRVYPKLAIGKSS 554

Query: 552 GLYAFNNGTESVTITKLRAWSLKKAQI 570
            L+AFN G +SV +  L AWS+  AQI
Sbjct: 555 HLFAFNYGYQSVDVLNLNAWSMNSAQI 580

BLAST of Csor.00g084170 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 790.4 bits (2040), Expect = 9.5e-229
Identity = 383/570 (67.19%), Postives = 446/570 (78.25%), Query Frame = 0

Query: 12  LCFLLCHGALKLQASHHVY--TPQPSRLLLHHHEQPYRTSCHFQPPKNWI---NGPMIYK 71
           L  L+ +  + L+ASHHVY    Q +        QPYRT  HFQPPKNW+   NGPMIYK
Sbjct: 15  LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWMNDPNGPMIYK 74

Query: 72  GIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGSATILPG 131
           GIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH  AI+PS P DINGCWSGSATILP 
Sbjct: 75  GIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILPN 134

Query: 132 DKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSSSFRDPT 191
            KP ILYTGI+PKNQQVQN+A PKNLSDPYLREW KSP NPLMAP   N IN+SSFRDPT
Sbjct: 135 GKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDPT 194

Query: 192 TAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMWECPDFFPVA 251
           TAWLG D +WRVIIGSKI  RGLAI + SKDF+KW++   PLHY DG GMWECPDFFPV 
Sbjct: 195 TAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWECPDFFPVT 254

Query: 252 KNGRAGVDTSVSGQH---VKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSIEDYRGL 311
           + G  GV+TS  G+    +KHVLK+SLDDTKHD YT+GTYD  KD +VPD G   D    
Sbjct: 255 RFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGFKMDGTAP 314

Query: 312 RYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASGKQL 371
           RYDYGKYYASKTFFD  K RR+LWGW NESSSV+DD++KGWSG+Q IPR +WLD SGKQL
Sbjct: 315 RYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQL 374

Query: 372 IQWPIMEIQKLRNDKVK-LADKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQKLKAE 431
           IQWP+ E+++LR  +VK L +KVLK GS + V GVTAAQADV+V FKV+   KA  ++  
Sbjct: 375 IQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPS 434

Query: 432 WKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFK---RQDKYVVLMCSDQ 491
           W DPQL+CS+M  + K G+GPFGL+VLAS NLEEYTSV+FRIFK     +KYVVLMCSDQ
Sbjct: 435 WTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQ 494

Query: 492 SRSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGKACITARVYPRLAIG 551
           SRSSL  +NDKTTYGAF+D++P  + +SLR+LIDHS+VESFG KG+ACIT+RVYP+LAIG
Sbjct: 495 SRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACITSRVYPKLAIG 554

Query: 552 DKAGLYAFNNGTESVTITKLRAWSLKKAQI 570
             + L+AFN G +SV +  L AWS+  AQI
Sbjct: 555 KSSHLFAFNYGYQSVDVLNLNAWSMNSAQI 583

BLAST of Csor.00g084170 vs. TAIR 10
Match: AT1G55120.1 (beta-fructofuranosidase 5 )

HSP 1 Score: 723.8 bits (1867), Expect = 1.1e-208
Identity = 349/538 (64.87%), Postives = 407/538 (75.65%), Query Frame = 0

Query: 44  QPYRTSCHFQPPKNWI---NGPMIYKGIYHLFYQYNPKGAVWG-NIVWAHSTSTDLINWK 103
           QPYRT  HFQP KNW+   NGPMIYKGIYHLFYQYNP GAVW   IVW HSTS DL+NW 
Sbjct: 36  QPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWI 95

Query: 104 PHDYAIYPSQPSDINGCWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLRE 163
               A  PSQPSDINGCWSGS TILP  KP ILYTGI+    QVQN+AVP N+SDPYLRE
Sbjct: 96  SQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLRE 155

Query: 164 WVKSPKNPLMAPTPQNQINSSSFRDPTTAWLGPDGEWRVIIGSKI-DARGLAIMFRSKDF 223
           W K P+NPLM     N IN   FRDPTTAWLG DGEWRVI+GS   D RGLAI+++S+DF
Sbjct: 156 WSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDF 215

Query: 224 VKWDQVDHPLHYADGMGMWECPDFFPVAKNGRAGVDTSVSGQH-VKHVLKVSLDDTKHDQ 283
             W Q   PLHY D  GMWECPDFFPV+  G  GV+TS  G++ +KHVLKVSL +T HD 
Sbjct: 216 FNWTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDY 275

Query: 284 YTVGTYDLKKDIYVPDKGSIEDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVD 343
           YT+G+YD +KD+YVPD G +++    R DYGKYYASKTF+DD KKRR+LWGWVNESS   
Sbjct: 276 YTIGSYDREKDVYVPDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAK 335

Query: 344 DDIKKGWSGVQAIPRTVWLDASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGV 403
           DDI+KGWSG+Q+ PR +WLD SGK+L+QWPI EI+ LR  +V    KVLK GS + V GV
Sbjct: 336 DDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGV 395

Query: 404 TAAQADVDVSFKVKSFNKAQKLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEY 463
           TAAQADV+VSFKVK   KA  ++  W DPQ +CSQ   +   G+GPFGL+VLASN++EEY
Sbjct: 396 TAAQADVEVSFKVKELEKADVIEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEY 455

Query: 464 TSVFFRIFKRQD------KYVVLMCSDQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSL 523
           TSV+FRIFK  D      KYVVLMCSDQSRSSLN  NDK+T+GAF+ +DP  + ISLR+L
Sbjct: 456 TSVYFRIFKSNDDTNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTL 515

Query: 524 IDHSIVESFGSKGKACITARVYPRLAIGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 570
           IDHSIVES+G  G+ CIT+RVYP+LAIG+ A L+ FN GT+SV I  L AWSLK AQI
Sbjct: 516 IDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQI 573

BLAST of Csor.00g084170 vs. TAIR 10
Match: AT3G13784.1 (cell wall invertase 5 )

HSP 1 Score: 704.9 bits (1818), Expect = 5.3e-203
Identity = 340/532 (63.91%), Postives = 407/532 (76.50%), Query Frame = 0

Query: 44  QPYRTSCHFQPPKNWINGPMIYKGIYHLFYQYNPKGAVW--GNIVWAHSTSTDLINWKPH 103
           QPYRT  HFQPPKNW+NGPMIYKGIYHLFYQ+N  GAV      VW H+TSTDLINW   
Sbjct: 40  QPYRTGYHFQPPKNWMNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTDLINWITL 99

Query: 104 DYAIYPSQPSDINGCWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWV 163
             AI PS+PSDINGCWSGS TILP  KP ILYTG +  N+QVQNL  PKNL+DPYLR W 
Sbjct: 100 SPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTDPYLRHWT 159

Query: 164 KSPKNPLMAPTPQNQINSSSFRDPTTAWLGPDGEWRVIIGSKIDARGLAIMFRSKDFVKW 223
           KSP+NPL+ P+P N INSS+FRDPTTAW G DG WR+  GS+   RGLAI+  SKDFV W
Sbjct: 160 KSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSKDFVIW 219

Query: 224 DQVDHPLHYADGMGMWECPDFFPVAKNGRAGVDTSV-SGQHVKHVLKVSLDDTKHDQYTV 283
            Q   PLHY DG GMWECPDFFPVA+    G+DTS  SG  VKHVLKVSL DT +D YT+
Sbjct: 220 KQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDTFNDYYTI 279

Query: 284 GTYDLKKDIYVPDKGSIEDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDI 343
           GTYD  +D+YVPDKG ++D    RYDYGK+YASKTF+D   +RR+LWGWVNESS   D+I
Sbjct: 280 GTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNESSPEKDNI 339

Query: 344 KKGWSGVQAIPRTVWLDASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAA 403
           KKGW+G+QAIPR VWLD SGK+L+QWP+ EI++LR  +VK  +K+LK GS++ V GVTA 
Sbjct: 340 KKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVMEVHGVTAP 399

Query: 404 QADVDVSFKVKSFN--KAQKLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYT 463
           QADV+V FKV  F+  KA  ++  W DPQL+CSQ  A++  G+GPFGL+VLAS N+EEYT
Sbjct: 400 QADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRNASS--GLGPFGLMVLASKNMEEYT 459

Query: 464 SVFFRIFK---RQDKYVVLMCSDQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHS 523
           SV  RIF+      ++VV+MCSDQS SSL   NDKTTYGAFLD+ P +  ISLR+LID S
Sbjct: 460 SVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISPYQP-ISLRTLIDKS 519

Query: 524 IVESFGSKGKACITARVYPRLAIGDKAGLYAFNNGTESVTITKLRAWSLKKA 568
           IVESFG KGK CIT+RVYP+LAIG++  L+AFN G+++V +  L AWS+K +
Sbjct: 520 IVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMKSS 568

BLAST of Csor.00g084170 vs. TAIR 10
Match: AT1G55120.3 (beta-fructofuranosidase 5 )

HSP 1 Score: 700.7 bits (1807), Expect = 9.9e-202
Identity = 337/524 (64.31%), Postives = 396/524 (75.57%), Query Frame = 0

Query: 55  PKNWINGPMIYKGIYHLFYQYNPKGAVWG-NIVWAHSTSTDLINWKPHDYAIYPSQPSDI 114
           P   ++GPMIYKGIYHLFYQYNP GAVW   IVW HSTS DL+NW     A  PSQPSDI
Sbjct: 35  PNVSVSGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDI 94

Query: 115 NGCWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP 174
           NGCWSGS TILP  KP ILYTGI+    QVQN+AVP N+SDPYLREW K P+NPLM    
Sbjct: 95  NGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNA 154

Query: 175 QNQINSSSFRDPTTAWLGPDGEWRVIIGSKI-DARGLAIMFRSKDFVKWDQVDHPLHYAD 234
            N IN   FRDPTTAWLG DGEWRVI+GS   D RGLAI+++S+DF  W Q   PLHY D
Sbjct: 155 VNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYED 214

Query: 235 GMGMWECPDFFPVAKNGRAGVDTSVSGQH-VKHVLKVSLDDTKHDQYTVGTYDLKKDIYV 294
             GMWECPDFFPV+  G  GV+TS  G++ +KHVLKVSL +T HD YT+G+YD +KD+YV
Sbjct: 215 LTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYV 274

Query: 295 PDKGSIEDYRGLRYDYGKYYASKTFFDDQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIP 354
           PD G +++    R DYGKYYASKTF+DD KKRR+LWGWVNESS   DDI+KGWSG+Q+ P
Sbjct: 275 PDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFP 334

Query: 355 RTVWLDASGKQLIQWPIMEIQKLRNDKVKLADKVLKKGSIIAVKGVTAAQADVDVSFKVK 414
           R +WLD SGK+L+QWPI EI+ LR  +V    KVLK GS + V GVTAAQADV+VSFKVK
Sbjct: 335 RKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVK 394

Query: 415 SFNKAQKLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQD-- 474
              KA  ++  W DPQ +CSQ   +   G+GPFGL+VLASN++EEYTSV+FRIFK  D  
Sbjct: 395 ELEKADVIEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDT 454

Query: 475 ----KYVVLMCSDQSRSSLNANNDKTTYGAFLDVDPVRKNISLRSLIDHSIVESFGSKGK 534
               KYVVLMCSDQSRSSLN  NDK+T+GAF+ +DP  + ISLR+LIDHSIVES+G  G+
Sbjct: 455 NKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGR 514

Query: 535 ACITARVYPRLAIGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 570
            CIT+RVYP+LAIG+ A L+ FN GT+SV I  L AWSLK AQI
Sbjct: 515 TCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQI 558

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q438661.3e-22767.19Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3... [more]
Q430898.4e-21464.68Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 P... [more]
Q70XE65.5e-21362.41Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1[more]
Q67XZ31.5e-20764.87Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3... [more]
Q9LIB93.1e-20063.55Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
KAG6575468.10.0100.00Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosp... [more]
XP_022954013.10.096.51beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata][more]
XP_023547447.10.095.81beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita pepo subsp. ... [more]
XP_022954011.10.095.98beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata] >X... [more]
KAG6575469.10.095.96Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
A0A6J1GPY10.096.51beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata O... [more]
A0A6J1GPU70.095.98beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata O... [more]
A0A6J1JP620.091.08beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita maxima OX=... [more]
A0A0A0K7610.081.46Cell wall invertase 5 OS=Cucumis sativus OX=3659 GN=Csa_7G450770 PE=2 SV=1[more]
A0A6J1DM230.081.12beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Momordica charantia ... [more]
Match NameE-valueIdentityDescription
AT3G13790.22.3e-23067.55Glycosyl hydrolases family 32 protein [more]
AT3G13790.19.5e-22967.19Glycosyl hydrolases family 32 protein [more]
AT1G55120.11.1e-20864.87beta-fructofuranosidase 5 [more]
AT3G13784.15.3e-20363.91cell wall invertase 5 [more]
AT1G55120.39.9e-20264.31beta-fructofuranosidase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001362Glycoside hydrolase, family 32SMARTSM00640glyco_32coord: 51..524
e-value: 3.1E-244
score: 827.4
NoneNo IPR availableGENE3D2.60.120.560coord: 379..564
e-value: 3.3E-80
score: 270.2
NoneNo IPR availablePANTHERPTHR31953BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATEDcoord: 3..569
NoneNo IPR availablePANTHERPTHR31953:SF51CELL-WALL INVERTASEcoord: 3..569
NoneNo IPR availableCDDcd18624GH32_Fruct1-likecoord: 57..356
e-value: 2.51371E-167
score: 475.338
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilyGENE3D2.115.10.20Glycosyl hydrolase domain; family 43coord: 55..378
e-value: 8.0E-115
score: 385.8
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilySUPERFAMILY75005Arabinanase/levansucrase/invertasecoord: 43..368
IPR013148Glycosyl hydrolase family 32, N-terminalPFAMPF00251Glyco_hydro_32Ncoord: 51..367
e-value: 6.5E-103
score: 344.5
IPR013189Glycosyl hydrolase family 32, C-terminalPFAMPF08244Glyco_hydro_32Ccoord: 370..563
e-value: 1.3E-29
score: 103.2
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 367..566

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g084170.m01Csor.00g084170.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds