Csor.00g080280 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g080280
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionDNA polymerase epsilon subunit
LocationCsor_Chr01: 4125560 .. 4129924 (-)
RNA-Seq ExpressionCsor.00g080280
SyntenyCsor.00g080280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGAATCCACTCTGAGGCGAAAGGTTCAGAGGAAATCAAAAATCAGAGGTTACAGCATCAAACTAGATGCTCTTGCAGAAATTGTTTCCTTTGCCTCTCGTTTCGATGGCTGCGAAGACGACGCCATAGATTTGGTTCTCGACCATCTTCATGACGAATCCTGTAATGCTATATCTTTTTTTTTTCTTTCGTACTGTAGGTCCTTTCATTCCTTCTCATGGACGATTTCGTCGTTTTTTTGCTACCATGGCAGTGCAATCTTCGATAATAGACAAGGATGCGGTGCATCGGGTTGTCAGTATTCTACTTGCAGCTGATGAAGCGGGGGAAGAATGTCCCGATACCAGTACTAGTACATCGGCTCTTTGCATCATAGACGCTTTTGATATCTCCAAGTATCGTTATGATCCCATCAAGAAGATCTTCTACATGTATGTTTTCCCACCTTGTATATTTATCTGAGTAATTGGTGAACAATTGAAGAATGATAACCGCCAATTGGACTTTTGGGTAAACTCCCTGCAGAAAAGAGAGATGTGCATATAGTGGACTTCAACTGATAACTTGATTAAACCATTTCTTTTCCATTTTAGGCACACAGGAAGCCTTCCAATTCATGGTGATGCTTCTGCTAAAGCTGCTTTGTATAGAGATAGGTTTCTCCTACTTTCCCAGAGGCTCTCCCGAGATCAGCATTTCTCAAAACCCGCTTTTGACAGTGGCATGTCTCATTTTGGAAGCTGTGAGGTACTCCATTTCTCTTGATCTTGAACTTACCTTGGTACCCTCTGCAGTTGATCATGAGCATTTACGGTAATTTCTCTCGTGATATGTTCCCCAGATTTCTCCTGTTCAGTCTCTGGTGGGGCAAACTGGGAGGAAATGGGTCATGGGAGTGATCTCTCAGATGGAGGATGGTCACTTTTTCTTGGAGGACCTTACTGCGTCTGTGGAAATTAATTTATCTAGTGCTATATCCTTTACAGACATCCTCTATAATTTGGAAAAATCTAAACTTGTTTGATATTTGAAGATGACTTGGAATTTGATTTTCTTTAACAATCAGCACAAGATAACTACAGGCCTGTTTACAGAGAACACCATCGTTGTAGCAGAAGGAGAGATGCTCGTGGAGGGTATTTTCAAGGTTTGTGACATGCGTTTGCATTTAGGTTTTTTATAAAGAGCTGGTTGGAATTTTCTCCCAATGTTCTATGTTGGTGGTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGGGGGGGGGAGAGAGAGAGAGTGAGAGACCTTGCTTTCTTATAAACTTTTGGTTGCATTAATTATGCTATTTTCTCTACCAAATTATCATGGGTTTATCTAGTAGGTAATGAAGATTAATGTGAATAATAAAATGCGATCAAGTTATGGTGGCTACCTACCTAGAATTTAATATCTTATATGTTACCATGACAACTAATGTAGCAGAGGCATATGGTTGTCGAACTTGTTCATACTAGTTGGTTCTAACGCTCATAAATATATAAAAAGAATGCTATTTTTTTCAACTGGTCGTCCAATGTTCTCTTGCTAATGTGTACTTCTCAGCTTGCTTTTCAGAGTCTCACAATTTCTGCTATTTTAAAATAGTTGATTACTTAGAAAAACGTCTTTGCACATCATATATGTCCTTTTAAACATAAACTTAGAAATTAGGACACTTTAACCGAAAGGGGCTGGCCTTTTGTTCTTATAATGCTACTGAAAATAGCCTGAATTAAATTCCTTATGGACTCAAATAGTTTCCAAAGTTCGTACATGCTTATGAGATAATCATCATATAATGCTACGGTATGGATTTTAGTTCTGTTTGGTCCTGATAGGAGTTTTTCCTCACTCAGTTATATTTCATGTAGTTCTCAGGCAAGTGCCTAATACCACCTTTTGTTTAGATATTTAATATTTCGTTAGGTATACCAGAACTTAAGGTTACACAAAGATTTGAAATCTTTTCTTAAACATATTTTTGTTTCTTTGGGCAAATTCTGCTCACACTCTTCTCTGAAGGTTGAAATTTAGAACAAAACTGAAGGTGAAGGTGCAATTTTCCTTCCTTTATCCATGAGTACTACCTTCTAAAAGTTGAATTTGCTTGGGACTTTGTTCTTGTGATTTACTTTCTCACTTGCTAGCTAGCACAAGGATACCTGTTGAAACTTTTTGGAAGATGTTCAGCACTTGTTTCATAGTATATTTCAAATACCGTTCATATTAATAATGTGCTGATTTTTTGGGGGTATTTTGTTAAGGTTATTACATGTGGATTTCCTCCATTAGAGGACAGGGACAAGTCTCTTAAATTGCTAGCAGGCCAGGACTTCTTTGGAGGTGGTTCTCTGACTAAAGAGGAGACTGTATCCCTATGAAAAGGATCTTTTAATATGCATGCACTGGATTGATGTGCTTTTTTGTTGATATTTTATTATACTGAATTTTGGCTATTAAAGTGGTGTACGCACATGCATCCAATGCAATCAACTTGTTGCCCTTAATCATCAATAGCTCAGGCTTGCAGATCTGGAAAAGAGAGCCGTTAACGATATGTTTGTCATACTCTCTGACATTTGGTTGGATAGTGAAGAGGTGAAGAATAACCTCTTTATATTTTACGTGCATTGTGTGTCTGTGAAGCTTGGTGCCTTAACAGAATTTGTTCATTTGGTAGGCCATGGGAAAACTGGAGACAATTCTTGATGGGTTTGAGAATGTGGAAATTGTTCCTTCCTTGTTTGTTTTGATGGGGAATTTTTGTTCCCGTCCATGTAATCTTGCCTTCAATTCTTACTCAAGTCTCAGGTAAGTATTGTCACAATTGTTTTCTGCATGCATTGGCTGTTATTGACATGAGTTCAAGAGACATTATGAGTTTGTGTGTGCAGATTACAATTTGGTAAGCTAGGAAAAATGATAGCAGCTCGTCCACGGTTAAAAGAGCATAGCAAGTTTCTTTTTATTCCTGGTCCAGATGATGCAGGTTTGTGTAGTTATTTGTAAATAAGTTCCTCGCCTTCTGCTTAAATATACATGAAAATTTCTTAATTTTTCTTCCCATTGAATTTATAGGACCATCAACAGTTTTACCCAGGTGTGCTTTGCCCAAGTATTTAACCGAAGAGCTTCAGATGCACGTCCCAAATGCCATCTTCTCAAGTAACCCTTGCAGGTGAATACTTCGATTGCTTACTTATAAACCTAACGGTCGTCCTAGTCTTTCGCTGTAGTATGATACTCCATGAGGGGTTTCACTTCTGCAGATAACATTAGAAATGAATCTTTTTTTCCCTTTGGCAACAAAATTGTATTTAAAGATTCGAATGAAAAAAATGAATTCTTACCTCCAAACTTGTTAATATCAATTTTCATTCTAAATCTCTAGTTTCATCAAATTGTACCTCAAACTTAAATAAGTGTTGCAATTTTAATCTTAAACTCGAAAATGTACTGTAACTTTTACCCTCTAAATAGTTTTCATTTGAAAGATCATTAAAAAAGTTCAAATAATATTTTAGTTCTTGAACTTTCAAAATGTCCACTTTGGTCCCTATAGGTTCTTTTGTATTTTGAATTGATTAAAAACAACTTAACCAATTTCAGTGGAAATTATGTTCTCTTTGGTGAATGACGTGCCCCTTTTACTCTATAGTGGTGGATAATTGTCATATTTGGATGCAGGGTTAGGTTCTACACCCAAGAAATTGTATTTTTCCGACAGGACCTACTTTACAGAATGCGTCGTTCCTGTCTCATACCGCCATCTACAGAAGAAACAAGTGATCCTTTTGAACATGTGATTTTTCTTCCTTATATATATCTGTTATTTTGTCTAGTTATTTGTTGTATATTAATTCTAGCTCTTTTAGATATTTATATGTGGAGGCCCTAAGGTTGTTTCATGATTCTCTTGCAGCTTGTTGCTACCATAACTCATCAAAGTCATCTCTGCCCTCTTCCTCTGGTTATTCAACCAATTATCTGGAATTATGACCACTGTCTTCATCTTTATCCTACTCCTCATGTGGTACTTACCTGTATATATCTGAATCTAGATAGTGTTTTAAACGATCTACTTATCCACAAAATTGGAGACAGATAATGAACAGTCACTGCAGATGGATGATAATGTTCTGCTGTCACTGAACTGCATTCATTGCATAAACTTTTATTTGGCTTGTGCAGATAGTTCTGGGGGATAGAAGTAAGCAACAAGCATTCAAATACACAGGAATCACTTGCTTCAATCCCGGTTCCTTCACCAATGACAGCACCTTTGTGGCATATCGTCCTTGCAATCAAGAAGTTGAATTATCTGCCTTGTAA

mRNA sequence

ATGGACGAATCCACTCTGAGGCGAAAGGTTCAGAGGAAATCAAAAATCAGAGGTTACAGCATCAAACTAGATGCTCTTGCAGAAATTGTTTCCTTTGCCTCTCGTTTCGATGGCTGCGAAGACGACGCCATAGATTTGGTTCTCGACCATCTTCATGACGAATCCTTGCAATCTTCGATAATAGACAAGGATGCGGTGCATCGGGTTGTCAGTATTCTACTTGCAGCTGATGAAGCGGGGGAAGAATGTCCCGATACCAGTACTAGTACATCGGCTCTTTGCATCATAGACGCTTTTGATATCTCCAAGTATCGTTATGATCCCATCAAGAAGATCTTCTACATGCACACAGGAAGCCTTCCAATTCATGGTGATGCTTCTGCTAAAGCTGCTTTGTATAGAGATAGGTTTCTCCTACTTTCCCAGAGGCTCTCCCGAGATCAGCATTTCTCAAAACCCGCTTTTGACAGTGGCATGTCTCATTTTGGAAGCTGTGAGATTTCTCCTGTTCAGTCTCTGGTGGGGCAAACTGGGAGGAAATGGGTCATGGGAGTGATCTCTCAGATGGAGGATGGCCTGTTTACAGAGAACACCATCGTTGTAGCAGAAGGAGAGATGCTCGTGGAGGGTATTTTCAAGGTTATTACATGTGGATTTCCTCCATTAGAGGACAGGGACAAGTCTCTTAAATTGCTAGCAGGCCAGGACTTCTTTGGAGGTGGTTCTCTGACTAAAGAGGAGACTCTCAGGCTTGCAGATCTGGAAAAGAGAGCCGTTAACGATATGTTTGTCATACTCTCTGACATTTGGTTGGATAGTGAAGAGGCCATGGGAAAACTGGAGACAATTCTTGATGGGTTTGAGAATGTGGAAATTGTTCCTTCCTTGTTTGTTTTGATGGGGAATTTTTGTTCCCGTCCATGTAATCTTGCCTTCAATTCTTACTCAAGTCTCAGATTACAATTTGGTAAGCTAGGAAAAATGATAGCAGCTCGTCCACGGTTAAAAGAGCATAGCAAGTTTCTTTTTATTCCTGGTCCAGATGATGCAGGACCATCAACAGTTTTACCCAGGTGTGCTTTGCCCAAGTATTTAACCGAAGAGCTTCAGATGCACGTCCCAAATGCCATCTTCTCAAGTAACCCTTGCAGGGTTAGGTTCTACACCCAAGAAATTGTATTTTTCCGACAGGACCTACTTTACAGAATGCGTCGTTCCTGTCTCATACCGCCATCTACAGAAGAAACAAGTGATCCTTTTGAACATCTTGTTGCTACCATAACTCATCAAAGTCATCTCTGCCCTCTTCCTCTGGTTATTCAACCAATTATCTGGAATTATGACCACTGTCTTCATCTTTATCCTACTCCTCATGTGATAGTTCTGGGGGATAGAAGTAAGCAACAAGCATTCAAATACACAGGAATCACTTGCTTCAATCCCGGTTCCTTCACCAATGACAGCACCTTTGTGGCATATCGTCCTTGCAATCAAGAAGTTGAATTATCTGCCTTGTAA

Coding sequence (CDS)

ATGGACGAATCCACTCTGAGGCGAAAGGTTCAGAGGAAATCAAAAATCAGAGGTTACAGCATCAAACTAGATGCTCTTGCAGAAATTGTTTCCTTTGCCTCTCGTTTCGATGGCTGCGAAGACGACGCCATAGATTTGGTTCTCGACCATCTTCATGACGAATCCTTGCAATCTTCGATAATAGACAAGGATGCGGTGCATCGGGTTGTCAGTATTCTACTTGCAGCTGATGAAGCGGGGGAAGAATGTCCCGATACCAGTACTAGTACATCGGCTCTTTGCATCATAGACGCTTTTGATATCTCCAAGTATCGTTATGATCCCATCAAGAAGATCTTCTACATGCACACAGGAAGCCTTCCAATTCATGGTGATGCTTCTGCTAAAGCTGCTTTGTATAGAGATAGGTTTCTCCTACTTTCCCAGAGGCTCTCCCGAGATCAGCATTTCTCAAAACCCGCTTTTGACAGTGGCATGTCTCATTTTGGAAGCTGTGAGATTTCTCCTGTTCAGTCTCTGGTGGGGCAAACTGGGAGGAAATGGGTCATGGGAGTGATCTCTCAGATGGAGGATGGCCTGTTTACAGAGAACACCATCGTTGTAGCAGAAGGAGAGATGCTCGTGGAGGGTATTTTCAAGGTTATTACATGTGGATTTCCTCCATTAGAGGACAGGGACAAGTCTCTTAAATTGCTAGCAGGCCAGGACTTCTTTGGAGGTGGTTCTCTGACTAAAGAGGAGACTCTCAGGCTTGCAGATCTGGAAAAGAGAGCCGTTAACGATATGTTTGTCATACTCTCTGACATTTGGTTGGATAGTGAAGAGGCCATGGGAAAACTGGAGACAATTCTTGATGGGTTTGAGAATGTGGAAATTGTTCCTTCCTTGTTTGTTTTGATGGGGAATTTTTGTTCCCGTCCATGTAATCTTGCCTTCAATTCTTACTCAAGTCTCAGATTACAATTTGGTAAGCTAGGAAAAATGATAGCAGCTCGTCCACGGTTAAAAGAGCATAGCAAGTTTCTTTTTATTCCTGGTCCAGATGATGCAGGACCATCAACAGTTTTACCCAGGTGTGCTTTGCCCAAGTATTTAACCGAAGAGCTTCAGATGCACGTCCCAAATGCCATCTTCTCAAGTAACCCTTGCAGGGTTAGGTTCTACACCCAAGAAATTGTATTTTTCCGACAGGACCTACTTTACAGAATGCGTCGTTCCTGTCTCATACCGCCATCTACAGAAGAAACAAGTGATCCTTTTGAACATCTTGTTGCTACCATAACTCATCAAAGTCATCTCTGCCCTCTTCCTCTGGTTATTCAACCAATTATCTGGAATTATGACCACTGTCTTCATCTTTATCCTACTCCTCATGTGATAGTTCTGGGGGATAGAAGTAAGCAACAAGCATTCAAATACACAGGAATCACTTGCTTCAATCCCGGTTCCTTCACCAATGACAGCACCTTTGTGGCATATCGTCCTTGCAATCAAGAAGTTGAATTATCTGCCTTGTAA

Protein sequence

MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSIIDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSLPIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGRKWVMGVISQMEDGLFTENTIVVAEGEMLVEGIFKVITCGFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Homology
BLAST of Csor.00g080280 vs. ExPASy Swiss-Prot
Match: Q500V9 (DNA polymerase epsilon subunit B OS=Arabidopsis thaliana OX=3702 GN=DPB2 PE=1 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 3.1e-204
Identity = 356/526 (67.68%), Postives = 422/526 (80.23%), Query Frame = 0

Query: 4   STLRRKVQRKSKIRGYSIKLDALAEIVSFASRF-DGCEDDAIDLVLDHLHDESLQSSIID 63
           S  R+K+Q+K K RGY++K DAL EI+ FA +F D  + +AIDL+LD+L  E+ +SS +D
Sbjct: 5   SQKRKKIQKKFKNRGYNLKFDALDEILVFADQFPDDDDGEAIDLLLDNL-QETHKSSTVD 64

Query: 64  KDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSLPI 123
            ++V  +++ LL A  A EE    +TS S+L IIDAF + K+ YD +KK F  HT SLPI
Sbjct: 65  AESVRGLINRLLGAHNAPEE---PTTSASSLAIIDAFLVPKFGYDSVKKKFNEHTSSLPI 124

Query: 124 HGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGRKWV 183
           HG+ASAK ALYR+RF+LLSQR+SR +HFS+PAFD+ MS F + EIS +QSL+ Q GRKWV
Sbjct: 125 HGEASAKTALYRERFMLLSQRVSRAEHFSRPAFDAEMSQFENNEISSIQSLISQRGRKWV 184

Query: 184 MGVISQMED-----------------------GLFTENTIVVAEGEMLVEGIFKVITCGF 243
           MGVISQ+ED                       G FTENTI++AEGEM V GIF+VITCGF
Sbjct: 185 MGVISQLEDGHFYLEDLSASVEIDLSKAKITTGFFTENTIILAEGEMQVNGIFQVITCGF 244

Query: 244 PPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAMGK 303
           PPLEDRDK+LK  +  DFFGGG+LTKEE ++LADLE++AVND FVILSDIWLD EE M K
Sbjct: 245 PPLEDRDKTLKAHSEYDFFGGGTLTKEEMIKLADLERQAVNDTFVILSDIWLDDEEVMRK 304

Query: 304 LETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKEHS 363
           LET+LDGFE+VE VPSLFV MGNFCSRPCNL+F SYSSLR QFGKLG+MI   PRLKE+S
Sbjct: 305 LETVLDGFESVETVPSLFVFMGNFCSRPCNLSFGSYSSLREQFGKLGRMIGNHPRLKENS 364

Query: 364 KFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQDL 423
           +FLFIPGP+DAGPSTVLPRCALPKYLTEEL+  +PNAIFSSNPCRV+FY QEIVFFRQDL
Sbjct: 365 RFLFIPGPEDAGPSTVLPRCALPKYLTEELRNIIPNAIFSSNPCRVKFYNQEIVFFRQDL 424

Query: 424 LYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTPHV 483
           LYRMRRSCL+ PS+EET+DPF+HLV TITHQSHLCPLPL++QPIIWNYDH L LYPTPH 
Sbjct: 425 LYRMRRSCLVTPSSEETNDPFKHLVYTITHQSHLCPLPLMVQPIIWNYDHALRLYPTPHT 484

Query: 484 IVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 506
           IVLGD+S+Q+  K+ G TCFNPGSF+ DSTFVAYRP  QEVELSAL
Sbjct: 485 IVLGDKSEQEVCKFGGTTCFNPGSFSTDSTFVAYRPSTQEVELSAL 526

BLAST of Csor.00g080280 vs. ExPASy Swiss-Prot
Match: Q5ZKQ6 (DNA polymerase epsilon subunit 2 OS=Gallus gallus OX=9031 GN=POLE2 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 8.9e-74
Identity = 179/544 (32.90%), Postives = 281/544 (51.65%), Query Frame = 0

Query: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCE-DDAIDLVLDHLHDESLQSS 60
           M+   LRR++    ++RG  ++ +A+  +       +  E +D ID ++D +  + L ++
Sbjct: 1   MEPERLRRRLGSAFRVRGLLLRAEAVKYLTETLESLNEEEFEDVIDKIIDAVEKQPLSTN 60

Query: 61  IIDKDAVHRVVSILLAADEAGEECPDT--STSTSALCIIDAFDISKYRYDPIKKIFY-MH 120
           +I+   V   V          +EC      T  +   II AFDI +Y Y+P +K F  + 
Sbjct: 61  MIELSTVEAAV----------QECSQALDETIKNVFNIIGAFDIPRYIYNPDRKRFVPLS 120

Query: 121 TGSLPI---HGDASAKAALYRDRFLLLSQRLSRDQHFSKPAF----DSGMSHFGSCEISP 180
             +LP+    G A  KA L+R+R+ +L QR  R + F+  A     D   S F   ++  
Sbjct: 121 MTNLPVPNLFGTARDKAELFRERYSILQQRTHRHELFTPAAVVIHPDDNRSKF---QLKT 180

Query: 181 VQSLVGQTGR---KWVMGVISQMED-----------------------GLFTENTIVVAE 240
           V++L+G T +     V+G+I+Q+++                       GL+TE+  V+AE
Sbjct: 181 VETLLGNTAKVREVIVLGMITQLKEGKYFLEDPTGVVQLDLSKAQFHSGLYTESCFVLAE 240

Query: 241 GEMLVEGIFKVITCGFPPLEDRDKSLKLLAGQDFFGG-GSLTKEETLRLADLEKRAVNDM 300
           G    + +F V   GFPP E    +       +FFGG  S + + + +L  LE    + M
Sbjct: 241 G-WYEDEVFHVNAFGFPPTEPSATTRAFYGNINFFGGPSSASVKASAKLKQLEDENEDAM 300

Query: 301 FVILSDIWLDSEEAMGKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQF 360
           FV LSD+WLD  E + KL T+  G+ +    P+ F   GNF S P     N   SL+   
Sbjct: 301 FVFLSDVWLDQAEVLEKLHTMFSGYSSAP--PTCFFFCGNFSSAP--YGKNQIQSLKGSL 360

Query: 361 GKLGKMIAARPRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNP 420
             L  +I   P + + S+F+F+PGP+D GP +VLPR  L + +T+E +  VP ++F++NP
Sbjct: 361 KALADIICEYPSIHKSSRFVFVPGPEDPGPGSVLPRPPLAENITQEFRQLVPFSVFTTNP 420

Query: 421 CRVRFYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQP 480
           CRV++ TQEI+ FR+DL+ +M R+C+  PS+  + D   H V TI  Q HL PLPL + P
Sbjct: 421 CRVQYCTQEIIIFREDLVNKMCRNCVRFPSS--SMDIPNHFVKTILSQGHLTPLPLYVSP 480

Query: 481 IIWNYDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDS-TFVAYRPCNQEVE 506
           + W YD+ L +YP P ++V+ D+        T   C NPGSF     +F  + P N+ VE
Sbjct: 481 VYWAYDYALRVYPVPDMLVIADKYDPFTVTNTDCLCINPGSFPRSGFSFKVFYPSNKTVE 524

BLAST of Csor.00g080280 vs. ExPASy Swiss-Prot
Match: Q54Y85 (DNA polymerase epsilon subunit 2 OS=Dictyostelium discoideum OX=44689 GN=pole2 PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 5.4e-71
Identity = 164/520 (31.54%), Postives = 269/520 (51.73%), Query Frame = 0

Query: 7   RRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSIIDKDAV 66
           ++++ +   + G+SIK DA+  I++            ID +L +L       +I   +  
Sbjct: 6   KKQITKSFSLTGFSIKADAMKCIINRIKNEGYDITQLIDNLLKNLDKSQYLQTI---EGC 65

Query: 67  HRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTG-SLPIHGD 126
            +++  + ++D   +E         AL +IDAF+   + +D   K F         +HG 
Sbjct: 66  FKLIDTINSSDAIEKE---------ALKVIDAFNTPLFNFDSNSKQFIKSENFDRSLHGS 125

Query: 127 ASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGRKWVMGV 186
           +++K+ LYR R+L + QR  R+ +FS P   S         I+P+ SL+G TGRK V+G 
Sbjct: 126 SNSKSDLYRKRYLKVLQRTERNDYFSTPVLASDKLKNEYQTITPLSSLLGNTGRKHVLGT 185

Query: 187 ISQMED-----------------------GLFTENTIVVAEGEMLVEGIFKVITCGFPPL 246
           ISQ+E+                       G+ T N+I+ A GE  ++G+F       PP 
Sbjct: 186 ISQIEEDQFYIEDLNTNVKIDISKAKFEFGIVTINSIIQASGE-FIDGVFIADKIQLPPT 245

Query: 247 EDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAMGKLET 306
           E+R ++LK L   D FG     K+   +L   EK   ++  + LSD+WLDSE  M +L+ 
Sbjct: 246 EERCETLKFLQNIDMFGERP-QKKTMEQLIKYEKEKEDNSILFLSDVWLDSERVMERLDY 305

Query: 307 ILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKEHSKFL 366
           +  G++  +  P   +LMGNF   P  L   +   L+  F +L  +I   P + + ++F+
Sbjct: 306 LFGGYK--DCPPFAMILMGNFTEHP--LINGTQYQLKKYFNQLAMVIQKYPNIHQFTQFI 365

Query: 367 FIPGPDDAGPS--TVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQDLL 426
           F+PGP D   S   +LP+  +     ++    +P + F++NPCR+R+ +QEI+ FR DL 
Sbjct: 366 FVPGPTDPTGSLLNILPKFPISNVFIKDFISLIPKSTFTTNPCRIRYCSQEIIIFRDDLT 425

Query: 427 YRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTPHVI 486
            +MRR C++ PS  ++ D  +HL+  I   SHLC L L  +PI WNYDH + +YP P ++
Sbjct: 426 NKMRRHCILEPS--QSCDISQHLIELICSNSHLCNLTLEDKPIYWNYDHAMSVYPLPDLL 485

Query: 487 VLGDRSKQ-QAFKYTGITCFNPGSFTNDSTFVAYRPCNQE 500
           V+GD+S Q +  +  G    NP SF+ D +F  Y P N++
Sbjct: 486 VIGDKSNQYEHSRADGTYSMNPSSFSTDYSFAHYIPANKQ 505

BLAST of Csor.00g080280 vs. ExPASy Swiss-Prot
Match: A7YWS7 (DNA polymerase epsilon subunit 2 OS=Bos taurus OX=9913 GN=POLE2 PE=2 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.2e-70
Identity = 172/541 (31.79%), Postives = 273/541 (50.46%), Query Frame = 0

Query: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCE-DDAIDLVLDHLHDESLQSS 60
           M    LR +     K+RG  ++ +A+  +       +  E +DA++ ++D +  + L S+
Sbjct: 1   MAPERLRSRALSAFKLRGLMLRGEAVKYLTEALQSINEIELEDALEKIIDAVEKQPLSSN 60

Query: 61  IIDKDAVHRVVSILLAADEAGEECPDT--STSTSALCIIDAFDISKYRYDPIKKIF---Y 120
           +I++  V   V          +EC  +   T      II AFDI ++ Y+  +K F    
Sbjct: 61  MIERSVVEAAV----------QECSQSVDETIDHIFNIIGAFDIPRFVYNSERKKFLPLL 120

Query: 121 MHTGSLP-IHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGS-CEISPVQS 180
           M     P + G A  KA L+R+R+ +L QR  R + F+ P   S     GS  ++  +++
Sbjct: 121 MTNHPAPNLFGTARDKAELFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLKTIET 180

Query: 181 LVGQT---GRKWVMGVISQMED-----------------------GLFTENTIVVAEGEM 240
           L+G T   G   V+G+I+Q+++                       GL+TE+  V+AEG  
Sbjct: 181 LLGSTSKIGDVIVLGMITQLKEGKFFLEDPTGTIQLDLSKAQFHSGLYTESCFVLAEG-W 240

Query: 241 LVEGIFKVITCGFPPLEDRDKSLKLLAGQDFFGGGSLTKEET-LRLADLEKRAVNDMFVI 300
             + +F V   GFPP E    +       +FFGG S T  +T  +L  LE    + MFV 
Sbjct: 241 FEDQVFHVNAFGFPPTEPSSTTRAYYGNTNFFGGPSNTSVKTSAKLKQLEDENKDAMFVF 300

Query: 301 LSDIWLDSEEAMGKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKL 360
           +SD+WLD  E + KL  +  G+      P+ F+L GNF S P     N   +L+     L
Sbjct: 301 ISDVWLDQVEVLEKLHIMFSGYSPAP--PTCFILCGNFSSAP--YGKNQVQALKDSLKTL 360

Query: 361 GKMIAARPRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRV 420
             +I   P + + S+F+F+PGP+D G  ++LPR    + +T E +  VP ++F++NPCR+
Sbjct: 361 ADIICGYPNIHQSSRFVFVPGPEDPGFGSILPRPPFAESITNEFRQRVPFSVFTTNPCRI 420

Query: 421 RFYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIW 480
           ++ TQEI+ FR+DL+ +M R+C+  PS+    D   H V T+  Q HL PLPL + P+ W
Sbjct: 421 QYCTQEIIVFREDLVNKMCRNCVRFPSS--NLDIPNHFVKTVLSQGHLTPLPLYVCPVYW 480

Query: 481 NYDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDS-TFVAYRPCNQEVELSA 506
            YD+ L +YP P ++V+ D+        T   C NPGSF     +F  + P N+ VE S 
Sbjct: 481 AYDYALRVYPVPDLLVIADKYDPFTLTNTECLCINPGSFPRSGFSFKVFYPSNKVVEDSK 524

BLAST of Csor.00g080280 vs. ExPASy Swiss-Prot
Match: P56282 (DNA polymerase epsilon subunit 2 OS=Homo sapiens OX=9606 GN=POLE2 PE=1 SV=2)

HSP 1 Score: 259.6 bits (662), Expect = 7.3e-68
Identity = 170/541 (31.42%), Postives = 269/541 (49.72%), Query Frame = 0

Query: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCE-DDAIDLVLDHLHDESLQSS 60
           M    LR +     K+RG  ++ +A+  +          E +D ++ +++ +  + L S+
Sbjct: 1   MAPERLRSRALSAFKLRGLLLRGEAIKYLTEALQSISELELEDKLEKIINAVEKQPLSSN 60

Query: 61  IIDKDAVHRVVSILLAADEAGEECPDT--STSTSALCIIDAFDISKYRYDPIKKIF---Y 120
           +I++  V   V          +EC  +   T      II AFDI ++ Y+  +K F    
Sbjct: 61  MIERSVVEAAV----------QECSQSVDETIEHVFNIIGAFDIPRFVYNSERKKFLPLL 120

Query: 121 MHTGSLP-IHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGS-CEISPVQS 180
           M     P + G    KA ++R+R+ +L QR  R + F+ P   S     GS  ++  +++
Sbjct: 121 MTNHPAPNLFGTPRDKAEMFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLKTIET 180

Query: 181 LVGQT---GRKWVMGVISQMED-----------------------GLFTENTIVVAEGEM 240
           L+G T   G   V+G+I+Q+++                       GL+TE   V+AEG  
Sbjct: 181 LLGSTTKIGDAIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEG-W 240

Query: 241 LVEGIFKVITCGFPPLEDRDKSLKLLAGQDFFGGGSLTKEET-LRLADLEKRAVNDMFVI 300
             + +F V   GFPP E    +       +FFGG S T  +T  +L  LE+   + MFV 
Sbjct: 241 FEDQVFHVNAFGFPPTEPSSTTRAYYGNINFFGGPSNTSVKTSAKLKQLEEENKDAMFVF 300

Query: 301 LSDIWLDSEEAMGKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKL 360
           LSD+WLD  E + KL  +  G+      P+ F+L GNF S P     N   +L+     L
Sbjct: 301 LSDVWLDQVEVLEKLRIMFAGYSPAP--PTCFILCGNFSSAP--YGKNQVQALKDSLKTL 360

Query: 361 GKMIAARPRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRV 420
             +I   P + + S+F+F+PGP+D G  ++LPR  L + +T E +  VP ++F++NPCR+
Sbjct: 361 ADIICEYPDIHQSSRFVFVPGPEDPGFGSILPRPPLAESITNEFRQRVPFSVFTTNPCRI 420

Query: 421 RFYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIW 480
           ++ TQEI  FR+DL+ +M R+C+  PS+        H V TI  Q HL PLPL + P+ W
Sbjct: 421 QYCTQEITVFREDLVNKMCRNCVRFPSSNLAIP--NHFVKTILSQGHLTPLPLYVCPVYW 480

Query: 481 NYDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDS-TFVAYRPCNQEVELSA 506
            YD+ L +YP P ++V+ D+        T   C NPGSF     +F  + P N+ VE S 
Sbjct: 481 AYDYALRVYPVPDLLVIADKYDPFTTTNTECLCINPGSFPRSGFSFKVFYPSNKTVEDSK 524

BLAST of Csor.00g080280 vs. NCBI nr
Match: KAG6607399.1 (DNA polymerase epsilon subunit B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1018 bits (2632), Expect = 0.0
Identity = 505/505 (100.00%), Postives = 505/505 (100.00%), Query Frame = 0

Query: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60
           MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI
Sbjct: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60

Query: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120
           IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL
Sbjct: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120

Query: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGRK 180
           PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGRK 180

Query: 181 WVMGVISQMEDGLFTENTIVVAEGEMLVEGIFKVITCGFPPLEDRDKSLKLLAGQDFFGG 240
           WVMGVISQMEDGLFTENTIVVAEGEMLVEGIFKVITCGFPPLEDRDKSLKLLAGQDFFGG
Sbjct: 181 WVMGVISQMEDGLFTENTIVVAEGEMLVEGIFKVITCGFPPLEDRDKSLKLLAGQDFFGG 240

Query: 241 GSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEIVPSLFVLM 300
           GSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEIVPSLFVLM
Sbjct: 241 GSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEIVPSLFVLM 300

Query: 301 GNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKEHSKFLFIPGPDDAGPSTVLPRCA 360
           GNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKEHSKFLFIPGPDDAGPSTVLPRCA
Sbjct: 301 GNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKEHSKFLFIPGPDDAGPSTVLPRCA 360

Query: 361 LPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPF 420
           LPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPF
Sbjct: 361 LPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPF 420

Query: 421 EHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFN 480
           EHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFN
Sbjct: 421 EHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFN 480

Query: 481 PGSFTNDSTFVAYRPCNQEVELSAL 505
           PGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 481 PGSFTNDSTFVAYRPCNQEVELSAL 505

BLAST of Csor.00g080280 vs. NCBI nr
Match: KAG7037068.1 (DNA polymerase epsilon subunit B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1007 bits (2604), Expect = 0.0
Identity = 504/521 (96.74%), Postives = 505/521 (96.93%), Query Frame = 0

Query: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60
           MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI
Sbjct: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60

Query: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120
           IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL
Sbjct: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120

Query: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGRK 180
           PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISP+QSLVGQTGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPIQSLVGQTGRK 180

Query: 181 WVMGVISQMEDG----------------LFTENTIVVAEGEMLVEGIFKVITCGFPPLED 240
           WVMGVISQMEDG                LFTENTIVVAEGEMLVEGIFKVITCGFPPLED
Sbjct: 181 WVMGVISQMEDGHFFLEDLTASHKITTGLFTENTIVVAEGEMLVEGIFKVITCGFPPLED 240

Query: 241 RDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAMGKLETIL 300
           RDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAMGKLETIL
Sbjct: 241 RDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAMGKLETIL 300

Query: 301 DGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKEHSKFLFI 360
           DGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKEHSKFLFI
Sbjct: 301 DGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKEHSKFLFI 360

Query: 361 PGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQDLLYRMR 420
           PGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQDLLYRMR
Sbjct: 361 PGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQDLLYRMR 420

Query: 421 RSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTPHVIVLGD 480
           RSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTPHVIVLGD
Sbjct: 421 RSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTPHVIVLGD 480

Query: 481 RSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 505
           RSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 481 RSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 521

BLAST of Csor.00g080280 vs. NCBI nr
Match: XP_022949454.1 (DNA polymerase epsilon subunit B [Cucurbita moschata])

HSP 1 Score: 1004 bits (2597), Expect = 0.0
Identity = 504/528 (95.45%), Postives = 505/528 (95.64%), Query Frame = 0

Query: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60
           MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI
Sbjct: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60

Query: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120
           IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL
Sbjct: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120

Query: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGRK 180
           PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISP+QSLVGQTGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPIQSLVGQTGRK 180

Query: 181 WVMGVISQMEDG-----------------------LFTENTIVVAEGEMLVEGIFKVITC 240
           WVMGVISQMEDG                       LFTENTIVVAEGEMLVEGIFKVITC
Sbjct: 181 WVMGVISQMEDGHFFLEDLTASVEINLSSAKITTGLFTENTIVVAEGEMLVEGIFKVITC 240

Query: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM 300
           GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM
Sbjct: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM 300

Query: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE 360
           GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE
Sbjct: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE 360

Query: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420
           HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ
Sbjct: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420

Query: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480
           DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP
Sbjct: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480

Query: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 505
           HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528

BLAST of Csor.00g080280 vs. NCBI nr
Match: XP_022998323.1 (DNA polymerase epsilon subunit B [Cucurbita maxima])

HSP 1 Score: 1003 bits (2592), Expect = 0.0
Identity = 501/528 (94.89%), Postives = 505/528 (95.64%), Query Frame = 0

Query: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60
           MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI
Sbjct: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60

Query: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120
           IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL
Sbjct: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120

Query: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGRK 180
           PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISP+QSLVGQTGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPIQSLVGQTGRK 180

Query: 181 WVMGVISQMEDG-----------------------LFTENTIVVAEGEMLVEGIFKVITC 240
           WVMGVISQMEDG                       LFTENT+VVAEGEMLVEGIFKVITC
Sbjct: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAKITTGLFTENTVVVAEGEMLVEGIFKVITC 240

Query: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM 300
           GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRA+NDMFVILSDIWLDSEEAM
Sbjct: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAINDMFVILSDIWLDSEEAM 300

Query: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE 360
           GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIA+RPRLKE
Sbjct: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIASRPRLKE 360

Query: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420
           HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ
Sbjct: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420

Query: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480
           DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP
Sbjct: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480

Query: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 505
           HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528

BLAST of Csor.00g080280 vs. NCBI nr
Match: XP_023525784.1 (DNA polymerase epsilon subunit B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1002 bits (2590), Expect = 0.0
Identity = 502/528 (95.08%), Postives = 505/528 (95.64%), Query Frame = 0

Query: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60
           MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI
Sbjct: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60

Query: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120
           IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISK+RYDPIKKIFYMHTGSL
Sbjct: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKFRYDPIKKIFYMHTGSL 120

Query: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGRK 180
           PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFD+GMSHFGSCEISP+QSLVGQTGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGMSHFGSCEISPIQSLVGQTGRK 180

Query: 181 WVMGVISQMEDG-----------------------LFTENTIVVAEGEMLVEGIFKVITC 240
           WVMGVISQMEDG                       LFTENTIVVAEGEMLVEGIFKVITC
Sbjct: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAKITTGLFTENTIVVAEGEMLVEGIFKVITC 240

Query: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM 300
           GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM
Sbjct: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM 300

Query: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE 360
           GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE
Sbjct: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE 360

Query: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420
           HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ
Sbjct: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420

Query: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480
           DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP
Sbjct: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480

Query: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 505
           HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528

BLAST of Csor.00g080280 vs. ExPASy TrEMBL
Match: A0A6J1GCV3 (DNA polymerase epsilon subunit OS=Cucurbita moschata OX=3662 GN=LOC111452793 PE=3 SV=1)

HSP 1 Score: 1004 bits (2597), Expect = 0.0
Identity = 504/528 (95.45%), Postives = 505/528 (95.64%), Query Frame = 0

Query: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60
           MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI
Sbjct: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60

Query: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120
           IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL
Sbjct: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120

Query: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGRK 180
           PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISP+QSLVGQTGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPIQSLVGQTGRK 180

Query: 181 WVMGVISQMEDG-----------------------LFTENTIVVAEGEMLVEGIFKVITC 240
           WVMGVISQMEDG                       LFTENTIVVAEGEMLVEGIFKVITC
Sbjct: 181 WVMGVISQMEDGHFFLEDLTASVEINLSSAKITTGLFTENTIVVAEGEMLVEGIFKVITC 240

Query: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM 300
           GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM
Sbjct: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM 300

Query: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE 360
           GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE
Sbjct: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE 360

Query: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420
           HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ
Sbjct: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420

Query: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480
           DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP
Sbjct: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480

Query: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 505
           HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528

BLAST of Csor.00g080280 vs. ExPASy TrEMBL
Match: A0A6J1KGF0 (DNA polymerase epsilon subunit OS=Cucurbita maxima OX=3661 GN=LOC111492993 PE=3 SV=1)

HSP 1 Score: 1003 bits (2592), Expect = 0.0
Identity = 501/528 (94.89%), Postives = 505/528 (95.64%), Query Frame = 0

Query: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60
           MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI
Sbjct: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60

Query: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120
           IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL
Sbjct: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120

Query: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGRK 180
           PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISP+QSLVGQTGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPIQSLVGQTGRK 180

Query: 181 WVMGVISQMEDG-----------------------LFTENTIVVAEGEMLVEGIFKVITC 240
           WVMGVISQMEDG                       LFTENT+VVAEGEMLVEGIFKVITC
Sbjct: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAKITTGLFTENTVVVAEGEMLVEGIFKVITC 240

Query: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM 300
           GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRA+NDMFVILSDIWLDSEEAM
Sbjct: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAINDMFVILSDIWLDSEEAM 300

Query: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE 360
           GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIA+RPRLKE
Sbjct: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIASRPRLKE 360

Query: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420
           HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ
Sbjct: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420

Query: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480
           DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP
Sbjct: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480

Query: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 505
           HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528

BLAST of Csor.00g080280 vs. ExPASy TrEMBL
Match: A0A6J1C7G4 (DNA polymerase epsilon subunit OS=Momordica charantia OX=3673 GN=LOC111009082 PE=3 SV=1)

HSP 1 Score: 912 bits (2358), Expect = 0.0
Identity = 454/528 (85.98%), Postives = 480/528 (90.91%), Query Frame = 0

Query: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60
           MD ST+RRKVQRKSKIRGYS+KLDAL EI+SFASRF G ED AID +LD LH ESLQSSI
Sbjct: 1   MDASTMRRKVQRKSKIRGYSLKLDALDEILSFASRFHGEEDAAIDFILDELHSESLQSSI 60

Query: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120
           I KDAVHR VS+LL ADE G++ PDT TSTSA  IID F I+++RYDPIKKIFY HTGSL
Sbjct: 61  IHKDAVHRAVSVLLGADEVGKQSPDTITSTSAPRIIDVFGITRFRYDPIKKIFYQHTGSL 120

Query: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGRK 180
           PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAF++GMSHFGSCEIS +QSLVGQTGR+
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFNTGMSHFGSCEISSIQSLVGQTGRR 180

Query: 181 WVMGVISQMEDG-----------------------LFTENTIVVAEGEMLVEGIFKVITC 240
           WVMGVISQMEDG                       LFTENTIVVAEGEMLVEGIF+VITC
Sbjct: 181 WVMGVISQMEDGHFYLEDLTASVEINLSNAKITTGLFTENTIVVAEGEMLVEGIFQVITC 240

Query: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM 300
           GFPPLEDRDKSLK+LAGQDFFG G+LTKEETLRLADLEKRA+NDMFVILSDIWLDSEEAM
Sbjct: 241 GFPPLEDRDKSLKMLAGQDFFGAGALTKEETLRLADLEKRAINDMFVILSDIWLDSEEAM 300

Query: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE 360
            KLETILDGFENVE+VPSLFVLMGNFCSRPCNLAFNS+SSLRLQFGKLGKMIAA PRL E
Sbjct: 301 RKLETILDGFENVEVVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAHPRLIE 360

Query: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420
           +SKFLFIPGPDDAGPSTVLPRCALPKYLTEEL+MHVPNAIFSSNPCR++FYTQEIVFFRQ
Sbjct: 361 NSKFLFIPGPDDAGPSTVLPRCALPKYLTEELRMHVPNAIFSSNPCRIKFYTQEIVFFRQ 420

Query: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480
           DLLYRMRRSCLIPPSTEETSDPFEHL+ATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP
Sbjct: 421 DLLYRMRRSCLIPPSTEETSDPFEHLIATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480

Query: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 505
           HVIVLGDRSKQQAFKYTGITCFNPGSF+ND TFVAYRPCNQEVELSAL
Sbjct: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFSNDGTFVAYRPCNQEVELSAL 528

BLAST of Csor.00g080280 vs. ExPASy TrEMBL
Match: A0A1S4DTQ5 (DNA polymerase epsilon subunit OS=Cucumis melo OX=3656 GN=LOC103501415 PE=3 SV=1)

HSP 1 Score: 911 bits (2354), Expect = 0.0
Identity = 455/529 (86.01%), Postives = 480/529 (90.74%), Query Frame = 0

Query: 1   MDESTLRRKVQRKSKIRG-YSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSS 60
           MD  TLR+KVQRK+KIRG YSIKL+AL EIVSF SRF G EDDAI+LVLD LH+ESL+S 
Sbjct: 1   MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60

Query: 61  IIDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGS 120
           I++KD VHRV+S LLAA+EA E  P+T TST+ALCIIDAFDISK+RYDPIKKIF+ HTG 
Sbjct: 61  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDAFDISKFRYDPIKKIFHRHTGD 120

Query: 121 LPIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGR 180
           LPIHGDA AKAALYRDRFLLLSQRLSRDQHFSKPAFD GMSHFGSCEISP+QSLVGQTGR
Sbjct: 121 LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180

Query: 181 KWVMGVISQMEDG-----------------------LFTENTIVVAEGEMLVEGIFKVIT 240
           KWVMGVISQMEDG                       LFTENTI+VAEGEMLVEGIF+V+T
Sbjct: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSNAKITTGLFTENTIIVAEGEMLVEGIFQVVT 240

Query: 241 CGFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEA 300
           CGFPPLE+RDKSLKLLAGQDFFGGG L KEETLRLADLEK+AVNDMFVILSDIWLDSEEA
Sbjct: 241 CGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVILSDIWLDSEEA 300

Query: 301 MGKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLK 360
           MGKLETILDGFENVE+VPSLFVLMGNFCS PCN+AFNS+SSLRLQFGKLGKMIAA PRL 
Sbjct: 301 MGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGKMIAAHPRLN 360

Query: 361 EHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFR 420
           +HSKF FIPGPDDAGPSTVLPRCALPKYLTEELQ+HVPNA FSSNPCRVRFYTQEIVFFR
Sbjct: 361 KHSKFXFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVRFYTQEIVFFR 420

Query: 421 QDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPT 480
           QDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPT
Sbjct: 421 QDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPT 480

Query: 481 PHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 505
           PHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 481 PHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 529

BLAST of Csor.00g080280 vs. ExPASy TrEMBL
Match: A0A5A7T342 (Glutamate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold112G00330 PE=3 SV=1)

HSP 1 Score: 910 bits (2351), Expect = 0.0
Identity = 456/537 (84.92%), Postives = 481/537 (89.57%), Query Frame = 0

Query: 1    MDESTLRRKVQRKSKIRG-YSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSS 60
            MD  TLR+KVQRK+KIRG YSIKL+AL EIVSF SRF G EDDAI+LVLD LH+ESL+S 
Sbjct: 614  MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 673

Query: 61   IIDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGS 120
            I++KD VHRV+S LLAA+EA E  P+T TST+ALCIIDAFDISK+RYDPIKKIF+ HTG 
Sbjct: 674  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDAFDISKFRYDPIKKIFHRHTGD 733

Query: 121  LPIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGR 180
            LPIHGDA AKAALYRDRFLLLSQRLSRDQHFSKPAFD GMSHFGSCEISP+QSLVGQTGR
Sbjct: 734  LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 793

Query: 181  KWVMGVISQMEDG-------------------------------LFTENTIVVAEGEMLV 240
            KWVMGVISQMEDG                               LFTENTI+VAEGEMLV
Sbjct: 794  KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDITTGLFTENTIIVAEGEMLV 853

Query: 241  EGIFKVITCGFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSD 300
            EGIF+V+TCGFPPLE+RDKSLKLLAGQDFFGGG L KEETLRLADLEK+AVNDMFVILSD
Sbjct: 854  EGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVILSD 913

Query: 301  IWLDSEEAMGKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKM 360
            IWLDSEEAMGKLETILDGFENVE+VPSLFVLMGNFCS PCN+AFNS+SSLRLQFGKLGKM
Sbjct: 914  IWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGKM 973

Query: 361  IAARPRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFY 420
            IAA PRL +HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQ+HVPNA FSSNPCRVRFY
Sbjct: 974  IAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVRFY 1033

Query: 421  TQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYD 480
            TQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYD
Sbjct: 1034 TQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYD 1093

Query: 481  HCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 505
            HCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 1094 HCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 1150

BLAST of Csor.00g080280 vs. TAIR 10
Match: AT5G22110.1 (DNA polymerase epsilon subunit B2 )

HSP 1 Score: 712.6 bits (1838), Expect = 2.2e-205
Identity = 356/526 (67.68%), Postives = 422/526 (80.23%), Query Frame = 0

Query: 4   STLRRKVQRKSKIRGYSIKLDALAEIVSFASRF-DGCEDDAIDLVLDHLHDESLQSSIID 63
           S  R+K+Q+K K RGY++K DAL EI+ FA +F D  + +AIDL+LD+L  E+ +SS +D
Sbjct: 5   SQKRKKIQKKFKNRGYNLKFDALDEILVFADQFPDDDDGEAIDLLLDNL-QETHKSSTVD 64

Query: 64  KDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSLPI 123
            ++V  +++ LL A  A EE    +TS S+L IIDAF + K+ YD +KK F  HT SLPI
Sbjct: 65  AESVRGLINRLLGAHNAPEE---PTTSASSLAIIDAFLVPKFGYDSVKKKFNEHTSSLPI 124

Query: 124 HGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPVQSLVGQTGRKWV 183
           HG+ASAK ALYR+RF+LLSQR+SR +HFS+PAFD+ MS F + EIS +QSL+ Q GRKWV
Sbjct: 125 HGEASAKTALYRERFMLLSQRVSRAEHFSRPAFDAEMSQFENNEISSIQSLISQRGRKWV 184

Query: 184 MGVISQMED-----------------------GLFTENTIVVAEGEMLVEGIFKVITCGF 243
           MGVISQ+ED                       G FTENTI++AEGEM V GIF+VITCGF
Sbjct: 185 MGVISQLEDGHFYLEDLSASVEIDLSKAKITTGFFTENTIILAEGEMQVNGIFQVITCGF 244

Query: 244 PPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAMGK 303
           PPLEDRDK+LK  +  DFFGGG+LTKEE ++LADLE++AVND FVILSDIWLD EE M K
Sbjct: 245 PPLEDRDKTLKAHSEYDFFGGGTLTKEEMIKLADLERQAVNDTFVILSDIWLDDEEVMRK 304

Query: 304 LETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKEHS 363
           LET+LDGFE+VE VPSLFV MGNFCSRPCNL+F SYSSLR QFGKLG+MI   PRLKE+S
Sbjct: 305 LETVLDGFESVETVPSLFVFMGNFCSRPCNLSFGSYSSLREQFGKLGRMIGNHPRLKENS 364

Query: 364 KFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQDL 423
           +FLFIPGP+DAGPSTVLPRCALPKYLTEEL+  +PNAIFSSNPCRV+FY QEIVFFRQDL
Sbjct: 365 RFLFIPGPEDAGPSTVLPRCALPKYLTEELRNIIPNAIFSSNPCRVKFYNQEIVFFRQDL 424

Query: 424 LYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTPHV 483
           LYRMRRSCL+ PS+EET+DPF+HLV TITHQSHLCPLPL++QPIIWNYDH L LYPTPH 
Sbjct: 425 LYRMRRSCLVTPSSEETNDPFKHLVYTITHQSHLCPLPLMVQPIIWNYDHALRLYPTPHT 484

Query: 484 IVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 506
           IVLGD+S+Q+  K+ G TCFNPGSF+ DSTFVAYRP  QEVELSAL
Sbjct: 485 IVLGDKSEQEVCKFGGTTCFNPGSFSTDSTFVAYRPSTQEVELSAL 526

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q500V93.1e-20467.68DNA polymerase epsilon subunit B OS=Arabidopsis thaliana OX=3702 GN=DPB2 PE=1 SV... [more]
Q5ZKQ68.9e-7432.90DNA polymerase epsilon subunit 2 OS=Gallus gallus OX=9031 GN=POLE2 PE=2 SV=1[more]
Q54Y855.4e-7131.54DNA polymerase epsilon subunit 2 OS=Dictyostelium discoideum OX=44689 GN=pole2 P... [more]
A7YWS71.2e-7031.79DNA polymerase epsilon subunit 2 OS=Bos taurus OX=9913 GN=POLE2 PE=2 SV=1[more]
P562827.3e-6831.42DNA polymerase epsilon subunit 2 OS=Homo sapiens OX=9606 GN=POLE2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
KAG6607399.10.0100.00DNA polymerase epsilon subunit B, partial [Cucurbita argyrosperma subsp. sororia... [more]
KAG7037068.10.096.74DNA polymerase epsilon subunit B [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022949454.10.095.45DNA polymerase epsilon subunit B [Cucurbita moschata][more]
XP_022998323.10.094.89DNA polymerase epsilon subunit B [Cucurbita maxima][more]
XP_023525784.10.095.08DNA polymerase epsilon subunit B [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1GCV30.095.45DNA polymerase epsilon subunit OS=Cucurbita moschata OX=3662 GN=LOC111452793 PE=... [more]
A0A6J1KGF00.094.89DNA polymerase epsilon subunit OS=Cucurbita maxima OX=3661 GN=LOC111492993 PE=3 ... [more]
A0A6J1C7G40.085.98DNA polymerase epsilon subunit OS=Momordica charantia OX=3673 GN=LOC111009082 PE... [more]
A0A1S4DTQ50.086.01DNA polymerase epsilon subunit OS=Cucumis melo OX=3656 GN=LOC103501415 PE=3 SV=1[more]
A0A5A7T3420.084.92Glutamate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
Match NameE-valueIdentityDescription
AT5G22110.12.2e-20567.68DNA polymerase epsilon subunit B2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.60.21.60coord: 272..501
e-value: 5.3E-6
score: 27.5
NoneNo IPR availableGENE3D1.10.8.60coord: 1..72
e-value: 5.9E-10
score: 41.1
IPR007185DNA polymerase alpha/delta/epsilon, subunit BPFAMPF04042DNA_pol_E_Bcoord: 263..470
e-value: 5.3E-47
score: 159.9
IPR016266DNA polymerase epsilon, subunit BPIRSFPIRSF000799DNA_pol_epsilon_2coord: 186..505
e-value: 2.5E-145
score: 482.6
coord: 1..192
e-value: 3.7E-34
score: 115.7
IPR016266DNA polymerase epsilon, subunit BPANTHERPTHR12708DNA POLYMERASE EPSILON SUBUNIT Bcoord: 7..504

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g080280.m01Csor.00g080280.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006261 DNA-dependent DNA replication
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0006536 glutamate metabolic process
biological_process GO:0051781 positive regulation of cell division
biological_process GO:0006260 DNA replication
cellular_component GO:0008622 epsilon DNA polymerase complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0070182 DNA polymerase binding
molecular_function GO:0004351 glutamate decarboxylase activity
molecular_function GO:0030170 pyridoxal phosphate binding