Csor.00g074210 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g074210
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	tRNA (Guanosine(18)-2'-O)-methyltransferase
Location	Csor_Chr11: 7051339 .. 7053629 (-)
RNA-Seq Expression	Csor.00g074210
Synteny	Csor.00g074210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCCTGCAGAATCGCAGTCCGACCTTCACTATTTCTACTTGGAAGCTGCAGTCCCCGTACAACTCAACGAATTCTCCCTTCTGTTTCCTTCTCCTCACCACCGCTTTCTTCCTACTCTCCATTCTTCACCGCCGTCTCAAACCATTGTACTCTTCTCTCACTCCCTCTCTTTCTCGCTCTCGGAGGCCATACAATATCTTTTGCATTTCTTTCTTAAAGCTGTTCTTGACTTTCCTTCTGTTTTGATTGCTTTACAGCCTCAAGAAACAACCCTAAGGCAGTTTCTACAAGATGTTTTTCCTCGACTTCGGCCGCTGCATCTTCAGTAGAAGTAACAGACTCTGTAGGAAGTGACCTAGAACCGCCGGAGATTCATACACAAGACACGGTGGAGCACCTTCTCACACACTCCTATGACGTTGCCAGGTTGATGAAGATGGAGCGGAGGCCGATTAATTTAGATGGAGATGAATATCCGCGTCAAGGGCGATGGTTTCCGTACCTGGATAAGTTTAGCTGTGAAAGTTCTTGGTTGAATAGTGGTGAGGTTTTGGACGCCTTGGATCCTCTCATATTGGACTCGAGGAAAGAGCGGTTCAAGAACGTCGTGCGACACAGGAGCTATTCGGTTTGTTTGGTGGTAGAAGGTTTGAGTGATTTCGGTAACGTCTCGGCGGCGTTTCGATCTGCCGATGCACTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTGCAAGAATGATTGAGGTGAAATTGTTTTCGAATACATTTGCCTTTTCCCCCTTGATTTTCCCAATGGAAAATTGTTTGGTTCTGGAATCCGGCAGCTGCTTCTTGCCCATTGTGCCGGCGGAATTTCATGAGTCTACATAGTGTGCTCTTACTTTGTTGTTCAATGGAGCACTTGAATTCAATCAATTAGTCGATTTGCATATCTGGTATTATATGATTAGGAGATTAAATTGATGAAAATGCATTTGATTTCCATGGTAATTGTTAGATATGGGTGACAATGTGACACATTCTACTTGCTAATAATGACAGAAATTTCCATTGAATACATTTTCATTCATGAGTCATAATCTTTCATTCCATGTTTCTTCTTCTGAGATTATTGCAATTCCATTGCAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCAACCCCAGAATGTTTTAGAATTCTGAAATCTCGGGGTTACAGAATAGTATCGACACATCTAGGATCGAATGCGGTATTGATTGATTTTGCAAACTTTCTCCCTTCCACAATGTCCCATGAAGTAGCTTTACATTGAAATCAATTCTGTTCTTATATCGATTGTCTTATGCCAATTTTATTATGTTTCAACCATTTTGAGCTTATCCTGACAGCTGAGTTGTGATTGTCCTGTTTTAGGTTTCCGTTTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGAAATGAAAGTAGGTAATTATCTTCTTTGCTTCAATAATACTGAGTTTGATCAAGTTTTTGTACACTTTTTAAATCTCTGCTAGTTGGGAGATATCAGTTATATTTAGCAAATCATAACTTAGTCCATGTTTAATAAACTGGCCTACACGTTTTGTTTTCCTGCAATTCTAGTCCGCTCATCCTTCGATTAGTGAGTTTCGAGTTGTGAATATTTTCCGAACCAGGAGACATTATACTTGTAGCTAGTTATGACCTTTACCATTTATGTTAGTATCTGAGGTTTAGTTTTCTTTGGTCTCAGGGGAATAAGTGATGAAGCCCTGGAGCTATCAGATTTGCATTGCAGTATTCCCATGAATGGAATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGATCGAACATCTCGATTGGTAACTCTAAAGTCATGTTTGATGTTTTGAATTCGTTTACGCCTAAGTGGTAGTTCAACTAGTTGGGGCATTACCAGGGGTGTTGTCTGGAAATATGTCGAACCGAGTCTTCCGGTGGGTCCGACGCAAAGGATGTCAAAATGGACATATACTGTTTTAGTACTGAATTGATGGTATGTTCTTTATCAAACTTTTCAGGGCTGCCATGGAGATCTCACATCAATAGAAAGGCAAATTCTACTGGCGGAGTTCTCACTACGGCATAACAAGAACGCAATCAGCGTTGCCCACGAGTATGCGAAGAGGAAGAGTAGTAGCACGAGCTCAGGTTCGGAGGTGTTGCTGTAG

mRNA sequence

ATGGCGTCCTGCAGAATCGCAGTCCGACCTTCACTATTTCTACTTGGAAGCTGCAGTCCCCGTACAACTCAACGAATTCTCCCTTCTGTTTCCTTCTCCTCACCACCGCTTTCTTCCTACTCTCCATTCTTCACCGCCGTCTCAAACCATTCCTCAAGAAACAACCCTAAGGCAGTTTCTACAAGATGTTTTTCCTCGACTTCGGCCGCTGCATCTTCAGTAGAAGTAACAGACTCTGTAGGAAGTGACCTAGAACCGCCGGAGATTCATACACAAGACACGGTGGAGCACCTTCTCACACACTCCTATGACGTTGCCAGGTTGATGAAGATGGAGCGGAGGCCGATTAATTTAGATGGAGATGAATATCCGCGTCAAGGGCGATGGTTTCCGTACCTGGATAAGTTTAGCTGTGAAAGTTCTTGGTTGAATAGTGGTGAGGTTTTGGACGCCTTGGATCCTCTCATATTGGACTCGAGGAAAGAGCGGTTCAAGAACGTCGTGCGACACAGGAGCTATTCGGTTTGTTTGGTGGTAGAAGGTTTGAGTGATTTCGGTAACGTCTCGGCGGCGTTTCGATCTGCCGATGCACTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCAACCCCAGAATGTTTTAGAATTCTGAAATCTCGGGGTTACAGAATAGTATCGACACATCTAGGATCGAATGCGGTTTCCGTTTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGAAATGAAAGTAGGGGAATAAGTGATGAAGCCCTGGAGCTATCAGATTTGCATTGCAGTATTCCCATGAATGGAATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGATCGAACATCTCGATTGGGCTGCCATGGAGATCTCACATCAATAGAAAGGCAAATTCTACTGGCGGAGTTCTCACTACGGCATAACAAGAACGCAATCAGCGTTGCCCACGAGTATGCGAAGAGGAAGAGTAGTAGCACGAGCTCAGGTTCGGAGGTGTTGCTGTAG

Coding sequence (CDS)

ATGGCGTCCTGCAGAATCGCAGTCCGACCTTCACTATTTCTACTTGGAAGCTGCAGTCCCCGTACAACTCAACGAATTCTCCCTTCTGTTTCCTTCTCCTCACCACCGCTTTCTTCCTACTCTCCATTCTTCACCGCCGTCTCAAACCATTCCTCAAGAAACAACCCTAAGGCAGTTTCTACAAGATGTTTTTCCTCGACTTCGGCCGCTGCATCTTCAGTAGAAGTAACAGACTCTGTAGGAAGTGACCTAGAACCGCCGGAGATTCATACACAAGACACGGTGGAGCACCTTCTCACACACTCCTATGACGTTGCCAGGTTGATGAAGATGGAGCGGAGGCCGATTAATTTAGATGGAGATGAATATCCGCGTCAAGGGCGATGGTTTCCGTACCTGGATAAGTTTAGCTGTGAAAGTTCTTGGTTGAATAGTGGTGAGGTTTTGGACGCCTTGGATCCTCTCATATTGGACTCGAGGAAAGAGCGGTTCAAGAACGTCGTGCGACACAGGAGCTATTCGGTTTGTTTGGTGGTAGAAGGTTTGAGTGATTTCGGTAACGTCTCGGCGGCGTTTCGATCTGCCGATGCACTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCAACCCCAGAATGTTTTAGAATTCTGAAATCTCGGGGTTACAGAATAGTATCGACACATCTAGGATCGAATGCGGTTTCCGTTTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGAAATGAAAGTAGGGGAATAAGTGATGAAGCCCTGGAGCTATCAGATTTGCATTGCAGTATTCCCATGAATGGAATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGATCGAACATCTCGATTGGGCTGCCATGGAGATCTCACATCAATAGAAAGGCAAATTCTACTGGCGGAGTTCTCACTACGGCATAACAAGAACGCAATCAGCGTTGCCCACGAGTATGCGAAGAGGAAGAGTAGTAGCACGAGCTCAGGTTCGGAGGTGTTGCTGTAG

Protein sequence

MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVSTRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDGDEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYAKRKSSSTSSGSEVLL

Homology

[Close]

BLAST of Csor.00g074210 vs. ExPASy Swiss-Prot
Match: O67577 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=trmH PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.8e-23
Identity = 56/174 (32.18%), Postives = 102/174 (58.62%), Query Frame = 0


Query: 152 LDPLILDSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDAS 211
           ++ L+L+ R +R + V+  R   + +  + + +  N SA  R+ DA+G   ++    +  
Sbjct: 3   MEYLVLEKRLKRLREVLEKRQKDLIVFADNVKNEHNFSAIVRTCDAVGVLYLYYYHAE-G 62

Query: 212 KRYRENRHVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPT 271
           K+ + N  ++ G+ KW+ IE  ++  +     K+RG++IV+T L   +V+  ++D++ PT
Sbjct: 63  KKAKINEGITQGSHKWVFIEKVDNPVQKLLEFKNRGFQIVATWLSKESVNFREVDYTKPT 122

Query: 272 AIVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR 326
            +VVGNE +G+S E +E++D    IPM GM  S NVSVA GI+++ A   R  +
Sbjct: 123 VLVVGNELQGVSPEIVEIADKKIVIPMYGMAQSLNVSVATGIILYEAQRQREEK 175

[Close]

BLAST of Csor.00g074210 vs. ExPASy Swiss-Prot
Match: P0AGJ3 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 OX=83334 GN=trmH PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.0e-21
Identity = 64/188 (34.04%), Postives = 100/188 (53.19%), Query Frame = 0


Query: 159 SRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 218
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 219 HVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNE 278
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 279 SRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCHGDLTSI 338
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 339 ERQILLAE 344
           E+Q LL E
Sbjct: 184 EQQRLLFE 189

[Close]

BLAST of Csor.00g074210 vs. ExPASy Swiss-Prot
Match: P0AGJ2 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=trmH PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.0e-21
Identity = 64/188 (34.04%), Postives = 100/188 (53.19%), Query Frame = 0


Query: 159 SRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 218
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 219 HVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNE 278
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 279 SRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCHGDLTSI 338
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 339 ERQILLAE 344
           E+Q LL E
Sbjct: 184 EQQRLLFE 189

[Close]

BLAST of Csor.00g074210 vs. ExPASy Swiss-Prot
Match: P0AGJ4 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri OX=623 GN=trmH PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.0e-21
Identity = 64/188 (34.04%), Postives = 100/188 (53.19%), Query Frame = 0


Query: 159 SRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 218
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 219 HVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNE 278
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 279 SRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCHGDLTSI 338
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 339 ERQILLAE 344
           E+Q LL E
Sbjct: 184 EQQRLLFE 189

[Close]

BLAST of Csor.00g074210 vs. ExPASy Swiss-Prot
Match: Q72GI1 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=trmH PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 2.2e-21
Identity = 56/161 (34.78%), Postives = 91/161 (56.52%), Query Frame = 0


Query: 158 DSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYREN 217
           ++R+ R + V+R R   + +++E +    N+SA  R+ DA+G    H V  + +      
Sbjct: 6   EARRRRIEEVLRRRQPDLTVLLENVHKPHNLSAILRTCDAVGVLEAHAV--NPTGGVPTF 65

Query: 218 RHVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGN 277
              S G+ KW+ + +     E FR LK RG+ + +T L  +A    ++D++ PTA++ G 
Sbjct: 66  NETSGGSHKWVYLRVHPDLHEAFRFLKERGFTVYATALREDARDFREVDYTKPTAVLFGA 125

Query: 278 ESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHA 319
           E  G+S+EAL L+D    IPM GMV S NVSVAA +++  A
Sbjct: 126 EKWGVSEEALALADGAIKIPMLGMVQSLNVSVAAAVILFEA 164

[Close]

BLAST of Csor.00g074210 vs. NCBI nr
Match: KAG6588498.1 (hypothetical protein SDJN03_17063, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 743 bits (1919), Expect = 1.47e-270
Identity = 375/375 (100.00%), Postives = 375/375 (100.00%), Query Frame = 0


Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS
Sbjct: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDG 120
           TRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDG
Sbjct: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
           DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300
           RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG
Sbjct: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360

Query: 361 KRKSSSTSSGSEVLL 375
           KRKSSSTSSGSEVLL
Sbjct: 361 KRKSSSTSSGSEVLL 375

[Close]

BLAST of Csor.00g074210 vs. NCBI nr
Match: XP_022933839.1 (uncharacterized protein LOC111441136 [Cucurbita moschata])

HSP 1 Score: 737 bits (1903), Expect = 4.03e-268
Identity = 373/375 (99.47%), Postives = 373/375 (99.47%), Query Frame = 0


Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS
Sbjct: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDG 120
            RCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHS DVARLMKMERRPINLDG
Sbjct: 61  IRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
           DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300
           RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG
Sbjct: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360

Query: 361 KRKSSSTSSGSEVLL 375
           KRKSSSTSSGSEVLL
Sbjct: 361 KRKSSSTSSGSEVLL 375

[Close]

BLAST of Csor.00g074210 vs. NCBI nr
Match: XP_023530258.1 (uncharacterized protein LOC111792877 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 732 bits (1889), Expect = 5.49e-266
Identity = 371/375 (98.93%), Postives = 372/375 (99.20%), Query Frame = 0


Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRIAVRPSLFLLGSCS RTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS
Sbjct: 1   MASCRIAVRPSLFLLGSCSSRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDG 120
           TRCFSSTSAAASSVEVTDSVGSDLEPPEIH QDTVE+LLTHS DVARLMKMERRPINLDG
Sbjct: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHAQDTVENLLTHSDDVARLMKMERRPINLDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
           DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300
           RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG
Sbjct: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360

Query: 361 KRKSSSTSSGSEVLL 375
           KRKSSSTSSGSEVLL
Sbjct: 361 KRKSSSTSSGSEVLL 375

[Close]

BLAST of Csor.00g074210 vs. NCBI nr
Match: XP_022969711.1 (uncharacterized protein LOC111468788 [Cucurbita maxima])

HSP 1 Score: 719 bits (1855), Expect = 8.36e-261
Identity = 364/375 (97.07%), Postives = 367/375 (97.87%), Query Frame = 0


Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRIAVRPSLFLLGSCS RTTQ ILPSVSF SPPLSSYSPFFTAVSNHSSRNNPKAV 
Sbjct: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60

Query: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDG 120
           TRCFSSTSAAASSVEVTD+VGSDLEPPEIHTQDTVEHLLTHS DVARLMKMERRPINLDG
Sbjct: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
            EYP QGRWFPYLDKFSCE+SWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE
Sbjct: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300
           RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHC+IPMNG
Sbjct: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360

Query: 361 KRKSSSTSSGSEVLL 375
           KRKSSSTSSG EVLL
Sbjct: 361 KRKSSSTSSGLEVLL 375

[Close]

BLAST of Csor.00g074210 vs. NCBI nr
Match: XP_038905162.1 (uncharacterized protein LOC120091275 isoform X2 [Benincasa hispida])

HSP 1 Score: 637 bits (1643), Expect = 1.56e-228
Identity = 326/375 (86.93%), Postives = 344/375 (91.73%), Query Frame = 0


Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRIA R SL LL SCS R  Q I PSVS  S PLSS+SPFFT V+N  SR+NP AVS
Sbjct: 1   MASCRIAARSSLILLESCSSRRPQSIFPSVSLPSLPLSSFSPFFTIVTNRLSRSNPNAVS 60

Query: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDG 120
           TRCF+S SAA SS+EV D+VG D EPPEIHTQDTVEHLLTHS DVARLMKMERRP  LDG
Sbjct: 61  TRCFASFSAATSSIEVADAVG-DREPPEIHTQDTVEHLLTHSDDVARLMKMERRPFELDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
            EYP +GRWFPYLDKFSC SSWLNSGE+L+ALDPLILDSRKERFKNVVR+RSYSVCLVVE
Sbjct: 121 AEYPHRGRWFPYLDKFSCGSSWLNSGEILEALDPLILDSRKERFKNVVRNRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300
           R+LKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG
Sbjct: 241 RVLKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTS ERQILLAEFSLRH+K+AISVA+E+A
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISVANEFA 360

Query: 361 KRKSSSTSSGSEVLL 375
           KRK S+ S G+EVLL
Sbjct: 361 KRKGSAIS-GTEVLL 373

[Close]

BLAST of Csor.00g074210 vs. ExPASy TrEMBL
Match: A0A6J1F0V5 (uncharacterized protein LOC111441136 OS=Cucurbita moschata OX=3662 GN=LOC111441136 PE=3 SV=1)

HSP 1 Score: 737 bits (1903), Expect = 1.95e-268
Identity = 373/375 (99.47%), Postives = 373/375 (99.47%), Query Frame = 0


Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS
Sbjct: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDG 120
            RCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHS DVARLMKMERRPINLDG
Sbjct: 61  IRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
           DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300
           RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG
Sbjct: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360

Query: 361 KRKSSSTSSGSEVLL 375
           KRKSSSTSSGSEVLL
Sbjct: 361 KRKSSSTSSGSEVLL 375

[Close]

BLAST of Csor.00g074210 vs. ExPASy TrEMBL
Match: A0A6J1HYK4 (uncharacterized protein LOC111468788 OS=Cucurbita maxima OX=3661 GN=LOC111468788 PE=3 SV=1)

HSP 1 Score: 719 bits (1855), Expect = 4.05e-261
Identity = 364/375 (97.07%), Postives = 367/375 (97.87%), Query Frame = 0


Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRIAVRPSLFLLGSCS RTTQ ILPSVSF SPPLSSYSPFFTAVSNHSSRNNPKAV 
Sbjct: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60

Query: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDG 120
           TRCFSSTSAAASSVEVTD+VGSDLEPPEIHTQDTVEHLLTHS DVARLMKMERRPINLDG
Sbjct: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
            EYP QGRWFPYLDKFSCE+SWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE
Sbjct: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300
           RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHC+IPMNG
Sbjct: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360

Query: 361 KRKSSSTSSGSEVLL 375
           KRKSSSTSSG EVLL
Sbjct: 361 KRKSSSTSSGLEVLL 375

[Close]

BLAST of Csor.00g074210 vs. ExPASy TrEMBL
Match: A0A0A0LC15 (SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G824750 PE=3 SV=1)

HSP 1 Score: 600 bits (1548), Expect = 1.98e-214
Identity = 312/375 (83.20%), Postives = 335/375 (89.33%), Query Frame = 0


Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRI  R SLFLL SCSPR TQRI  SV   S  L S+S FFT   N  SR+NP AVS
Sbjct: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFT---NRFSRSNPNAVS 60

Query: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDG 120
           TRCF+S SAA S +EV D V  D+EPPE+ TQDTVEHLLTHS DVARLMKMERR + LDG
Sbjct: 61  TRCFASISAATSPLEVEDIVA-DVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
            EYP +GRWFPYLDK+SC SSWLNSGE+L+ALD LILDSRKERFKNVVR+RSYSVCLVVE
Sbjct: 121 AEYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GL+DFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300
           +ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHCSIPMNG
Sbjct: 241 KILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVC+RTSRLGCHGDLTS ERQILLAEFSLRH+K+AIS+A+E A
Sbjct: 301 MVDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELA 360

Query: 361 KRKSSSTSSGSEVLL 375
           KRK S+ SS +EVLL
Sbjct: 361 KRKGSAISS-TEVLL 370

[Close]

BLAST of Csor.00g074210 vs. ExPASy TrEMBL
Match: A0A5A7UJ80 (tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007650 PE=3 SV=1)

HSP 1 Score: 584 bits (1506), Expect = 4.74e-208
Identity = 304/369 (82.38%), Postives = 328/369 (88.89%), Query Frame = 0


Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRI  R SL LL S SPR TQR+ PSV   S  L S SPFFT   N  SR+NP AVS
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFFT---NTLSRSNPNAVS 60

Query: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDG 120
           +RCF+S SAA S +E+ D V  D+EPPE+ TQDTVEHLLTHS DV+RLMKMERRP+ LDG
Sbjct: 61  SRCFASISAATSPLELADIVA-DVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
            EYP +GRWFPYLDK+S  SSWLNSGE+L+ALD LILD+RKERFKNVVR+RSYSVCLVVE
Sbjct: 121 AEYPHKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNST ECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTSECF 240

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300
           +ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHCSIPMNG
Sbjct: 241 KILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTS ERQILLAEFSLRH+K+AIS+A+E A
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELA 360

Query: 361 KRKSSSTSS 369
           KRK S+ SS
Sbjct: 361 KRKGSAISS 364

[Close]

BLAST of Csor.00g074210 vs. ExPASy TrEMBL
Match: A0A1S3B8M3 (tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486969 PE=3 SV=1)

HSP 1 Score: 584 bits (1506), Expect = 4.74e-208
Identity = 304/369 (82.38%), Postives = 328/369 (88.89%), Query Frame = 0


Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRI  R SL LL S SPR TQR+ PSV   S  L S SPFFT   N  SR+NP AVS
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFFT---NTLSRSNPNAVS 60

Query: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDG 120
           +RCF+S SAA S +E+ D V  D+EPPE+ TQDTVEHLLTHS DV+RLMKMERRP+ LDG
Sbjct: 61  SRCFASISAATSPLELADIVA-DVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
            EYP +GRWFPYLDK+S  SSWLNSGE+L+ALD LILD+RKERFKNVVR+RSYSVCLVVE
Sbjct: 121 AEYPHKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNST ECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTSECF 240

Query: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300
           +ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHCSIPMNG
Sbjct: 241 KILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360
           MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTS ERQILLAEFSLRH+K+AIS+A+E A
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELA 360

Query: 361 KRKSSSTSS 369
           KRK S+ SS
Sbjct: 361 KRKGSAISS 364

[Close]

BLAST of Csor.00g074210 vs. TAIR 10
Match: AT5G15390.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 395.2 bits (1014), Expect = 5.9e-110
Identity = 192/279 (68.82%), Postives = 232/279 (83.15%), Query Frame = 0


Query: 92  QDTVEHLL-THSYDVARLMKMERRPINLDGDEYPRQGRWFPYLDKFSCESSWLNSGEVLD 151
           +DTV+ LL T+  + + LMKMERR  +L   E   +G WFPY D+F C    L+S EVL+
Sbjct: 72  KDTVKGLLTTNRGEASSLMKMERR-CSLSNGEGDCRGSWFPYEDRFRCGEVHLSSREVLE 131

Query: 152 ALDPLILDSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDA 211
           A+ P +++ R +RF+ VV +RSYSVCLVVEGLSDFGN+SAAFRSADALG QSVHVVSCD+
Sbjct: 132 AVSPHMMEERTDRFRRVVENRSYSVCLVVEGLSDFGNISAAFRSADALGIQSVHVVSCDS 191

Query: 212 SKRYRENRHVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCP 271
           SKRY  NRHVSMGAEKWLDIE W++  ECF++LKSRGYRI +THLG + VS+YDMDWSCP
Sbjct: 192 SKRYNGNRHVSMGAEKWLDIEFWDTPKECFKVLKSRGYRIATTHLGMDTVSIYDMDWSCP 251

Query: 272 TAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHG 331
           TAIVVGNE RGISDEALELSDL CSIPMNGMVDSFNVSVAAGILMHHAV DRT+RLG HG
Sbjct: 252 TAIVVGNEGRGISDEALELSDLRCSIPMNGMVDSFNVSVAAGILMHHAVSDRTTRLGSHG 311

Query: 332 DLTSIERQILLAEFSLRHNKNAISVAHEYAKRKSSSTSS 370
           DL+  E++IL+AEFSLRH++++I +A+E+AKRK   ++S
Sbjct: 312 DLSEAEKEILMAEFSLRHSRSSICIAYEFAKRKQQHSTS 349

[Close]

BLAST of Csor.00g074210 vs. TAIR 10
Match: AT2G19870.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 60.1 bits (144), Expect = 4.5e-09
Identity = 49/202 (24.26%), Postives = 88/202 (43.56%), Query Frame = 0


Query: 144 NSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSV 203
           + G VLDA  PL L   KE          YS+ + ++ ++D  N+ A  RSA   G   V
Sbjct: 388 HQGLVLDA-SPLELVKVKELDPISSEEEKYSLWVALDEVTDPQNLGAIIRSAYFFGATGV 447

Query: 204 HVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVY 263
            V + +++         S G+ + +++    +  +        G+R+V   +   AV++ 
Sbjct: 448 VVCAKNSAPLSAVVSKASAGSLEVMELRYCKNMMQFLEASAENGWRVVGGSVSPKAVALN 507

Query: 264 DMDWSCPTAIVVGNESRG----ISDEALELSDLHCSIPMNG-----------------MV 323
           ++    PT +V+GNE  G    +     +L  +  ++P                     V
Sbjct: 508 EVLPGSPTILVLGNEGTGLRPLVERSCTDLVRISGNMPNEVAVTESDDAEGEGFRSFLAV 567

Query: 324 DSFNVSVAAGILMHHAVCDRTS 325
           +S NVSVAAG+ +HH + ++ S
Sbjct: 568 ESLNVSVAAGLFLHHLIGNKAS 588

[Close]

BLAST of Csor.00g074210 vs. TAIR 10
Match: AT4G38020.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 47.4 bits (111), Expect = 3.0e-05
Identity = 41/152 (26.97%), Postives = 72/152 (47.37%), Query Frame = 0


Query: 177 LVVEGLSDFGNVSAAFRSADALGFQSVHVV--SCDA--SKRYRENRHVS----MGAEKWL 236
           LV++ + D GN+    RSA A  +    ++   CD    K  R +R  S    + +  W 
Sbjct: 199 LVLDSIQDPGNLGTLVRSAMAFNWDGAFLLPGCCDPYNDKALRASRGASFQLPIVSGNWN 258

Query: 237 DIELWNSTPECFRILKSRGYRIVSTH----LGSNAVSVYDMDWSCPTAIVVGNESRGISD 296
            ++L  +    F++    G+   +T     +   +V         P  +++G+E  G+S+
Sbjct: 259 HLKLLENE---FQMKLLAGHPATTTQKLKPVSKLSVEFAQSLAEKPLCLILGSEGNGLSE 318

Query: 297 EALELSDLHCSIPMNGMVDSFNVSVAAGILMH 317
           +A ++  L  SIPM G  +S NVSVA GI ++
Sbjct: 319 QARKVCVL-VSIPMAGDFESLNVSVAGGIFLY 346

The following BLAST results are available for this feature:

BLAST of Csor.00g074210 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O67577	1.8e-23	32.18	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) OX=...	[more]
P0AGJ3	1.0e-21	34.04	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 OX=83334...	[more]
P0AGJ2	1.0e-21	34.04	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=...	[more]
P0AGJ4	1.0e-21	34.04	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri OX=623 GN=trmH ...	[more]
Q72GI1	2.2e-21	34.78	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain ATCC...	[more]

BLAST of Csor.00g074210 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6588498.1	1.47e-270	100.00	hypothetical protein SDJN03_17063, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022933839.1	4.03e-268	99.47	uncharacterized protein LOC111441136 [Cucurbita moschata]	[more]
XP_023530258.1	5.49e-266	98.93	uncharacterized protein LOC111792877 [Cucurbita pepo subsp. pepo]	[more]
XP_022969711.1	8.36e-261	97.07	uncharacterized protein LOC111468788 [Cucurbita maxima]	[more]
XP_038905162.1	1.56e-228	86.93	uncharacterized protein LOC120091275 isoform X2 [Benincasa hispida]	[more]

BLAST of Csor.00g074210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F0V5	1.95e-268	99.47	uncharacterized protein LOC111441136 OS=Cucurbita moschata OX=3662 GN=LOC1114411...	[more]
A0A6J1HYK4	4.05e-261	97.07	uncharacterized protein LOC111468788 OS=Cucurbita maxima OX=3661 GN=LOC111468788...	[more]
A0A0A0LC15	1.98e-214	83.20	SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G824...	[more]
A0A5A7UJ80	4.74e-208	82.38	tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3B8M3	4.74e-208	82.38	tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486...	[more]

BLAST of Csor.00g074210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G15390.1	5.9e-110	68.82	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT2G19870.1	4.5e-09	24.26	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT4G38020.1	3.0e-05	26.97	tRNA/rRNA methyltransferase (SpoU) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 151..358 e-value: 2.7E-58 score: 198.6
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 175..316 e-value: 2.6E-34 score: 118.4
None	No IPR available	PANTHER	PTHR43453:SF1	RRNA METHYLASE-LIKE PROTEIN	coord: 1..367
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	PANTHER	PTHR43453	RRNA METHYLASE-LIKE	coord: 1..367
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	HAMAP	MF_02060	tRNA_methyltr_TrmH	coord: 157..351 score: 26.743629
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	CDD	cd18092	SpoU-like_TrmH	coord: 159..322 e-value: 6.12423E-79 score: 237.751
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 159..324

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g074210.m01	Csor.00g074210.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
biological_process	GO:0006396	RNA processing
molecular_function	GO:0106050	tRNA 2'-O-methyltransferase activity
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0008173	RNA methyltransferase activity