Csor.00g070350 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g070350
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionAB hydrolase-1 domain-containing protein
LocationCsor_Chr14: 10474798 .. 10478762 (-)
RNA-Seq ExpressionCsor.00g070350
SyntenyCsor.00g070350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTCTGAAATGGTGCTTACCCTCACTGTCGCTTCACCAGCTTACGAGAACGCCAATGGCAGCGAAATGTGAAGGCCTTGGCCTTGGAGGAGTTTTATCCCATGGAAACTCTGGAGCATTCCCCTCGTTTCTTCCTAAAGAAGTCGGTAAAATCAAAGATCCATTTGCCAGAACCATGGCTCGCAAAATCGAGAGGCTACCGGTAAAATGATTTTTTCTCTATCCTTTTCTTGTGTTTTCTTGGACGAAATCAATGGTGCATTGATCTCGCAACGATGACTTTGCTTCTTCAGTTTCTGATTCTGTTTCTTTTTTGTTTTTGTCTGCCTTTTAATCGAGTGGTTTGAACATGAAACTTGTAGTATCTGCCTTTTTAAACTGCGTGCTTATTTCCGGTCGATTTTTGACAAGGAAGGCCAGCTGTAAGAATCGTTTGCAATGAAAATTTTACTGCTTAGAAACTGGACTCGAAAGCAATTCGCCGTTGTACAGTTGTCCATTGAAGATCCTTCGCCTTGAGTATATTGTTCTTTAGAGCAACTGTGATAAGAAACTTCCCTTGCATTACAAAGAATGGAAACAAGCAATATAACGCAAATTTATGTTTTGAAATCGTCAAATGGTTAAGGATCTTAACTCCGTCTCATTTCGGAGCTGATCAGTTCATCTACAATGGTATTCAAGTTTGTTGTTTGTCTTTAGCTACAGGATGGCACGTCCATTGTTTTATTGATATACAGAGTTGTATTTTTAGATATCATTCATCTGAATCTCTCATGTAGTACTCAATAACGACTTTTCTTACGCAATTGATTTTCTCCAATTTCACCTTGCGCTTCAATTCCTGCCCCTTCTGTCTTCAAGGTTACTACCAAGCCCAAGCCTGCACAATGATTCTTTATACTTGTTCTTTTTTGCAGATGACATTATCTGAAACTCGCATTATGAGCAGCTGCGTTAAACCAGTAATAAGAAGTGAGAAGAATCCGGTGGTTCTACTCCATTGCTTTGACAGGTATATTATGGAAGTAACGAGATTTTAAATTTTTTTTTTTTAATATGTACTCTTTCCATTTAACATGATATGTGCCGTTTATTTTTGTCCATGCACAGTTCTTGTTTGGAATGGAGTTATGCATTTCCTCTGCTTGAAGAGGCTGGTTTAGAAACGTGGGCTGTCGATGTTCTTGGATGGGGCTTTTCTGACTTGGGTGGGTTCATTTAAGAGTTATTATTCATTTGGTTATATTTTCTTCTGGCTACTAGTATTTTATAAACTGTAAAAAGGTTTCTCACACATATGTTTCCTGATTTTACTTATGCATACTAGATAGTCTTCCACCATGTAACGTCCAAACAAAGCGTTCTCATCTTTACCAGGTAAATCACTTTACCCTTTTACCTTCGTTTGTTTTAGAAAGATTATTGGTTATTCAATGTCTCCATTTATCCATGTGTTCTTTACGTCTTAAAAGTGCTTATAATTTGAGAATTGAAATTCTTTGTGAAGTTTTATTTGTTGTAATGGTTTGAATTTCGCAACAGTTGTTTTAGGCTGTAATTGAATTTTAAATTGGGTTTTAGTTTTGGAAGACCTACATCAAGAGGCCAATGGTGTTAGTTGGACCAAGCCTTGGAGCAGCGGTTGCAGTTGATTTTGCAGTTAATCATCCGGAAGCTGTAAGTGTAAGCTTTATATCTAGCTTAAGAGTAATTCAATGATCTTGACGATTTGTGCATGAAGAATGTGAGTAACCAAATGAGGCCTACAATCAGATTTAGGGCCTTCTCACACAAATTTCCTAAACAAATGCTTGTGTGCATTGTCAGCAATTTCGTACCTATTTTATTATATTTATATCAGATGGAGATTTATAGTTGGAAACTGGTTTATTAACATCATCCCTCATGATGCTTTCTATTTCATCCTGATAATATATACTTGCCAATCACAATTAATAGTTTATGTGAAACATAGTCTTTAAACTTACAGAAATGATGGTCCTTGGAGCCTTAAAATTCTTGATCCTGAATCATCCTCCAGGCTACTAATATATAGTTGATTTGTAATGTGTTCCTTACGCTTCTTAACTATTTCTTTGTGAGCTTTTAGTTGTGAATTCTTTTTCATGATTCTGTTTCACAGGTGGAAAAGCTGGTCTTGATCAATGCTTGTGTGTATAGTAAATGCACCGGGCACCCTACAAAGCTGCCGAAAGTGGTGGCATATGCTGGGGTAGGCCTCCATGGCACACAACTTAGTACATGCCAGTAATTAATATTCCATTTCTTTCTCGTACACTTCATCAATGTACAAATTACAATTTAACTATTTTAAACGACTTTTGGAAGATGATAAATTGGCGGAGTCAACAACTTTTTAGCCTTATGCTCTTCAAGTACTTAAGTTTCCAACTCTTCAATAGTGTCATGAAAAAATATTGTCGGAATTTATGTAACATAGTAAAATACATATGCAGATTTGAATTTCCTTAATCTTCATAGATCAAAATGCTTGTTTTGGGAAGGATGGTAGAGCCTAAAGCTTGTATAGCACTGACAGTTTTTTTTTTTTTTTTTTTTCCCAACGAATTGTCAGGTTTCACTGTTAAAAAGTATCCCATTGCGCCTCATTGCAAACAAGTTAACATTCAATGGACTTTCACTTGCTCGAAATCTAGAATGGACGAATGTAAGTTTCTCTTGATGTCCTTTCTGGAAAAGGTTGCTTTTGAATATGTCATTAATAAGGCATATAACCTATCAATCTCATCTCAGACACTTTGATGCTGATGGATGGCTTCTTTCTTTTTCCTGCCCTTGTTTTTCTCAAATCTTTTCGTTTACCACGTGAATCATGAAGACCTTTTTTGTAATCTTAAGTCACCTTTGACGAGTGTGAAATATGATCCATCAATTACTTTTTGACAGTAACAAAATATGTCATAAATATGAAAGTAAGATATTTCTGTTTAGTTGCTGATTTCATATCTTGTTTAGTGGGTGCGTGATGACGTGACAATCTACTTATAATGTTATTATACGGATTAGGTTGGCCGTTTGCACTGCCTATTGCCTTGGTGGGAAGATGCAACGGTTAATTTTATGAACAGCGGAGGATATAATGTCATTTCCCAGATTAAACAGGTATATCCATAAATACCCATTATGATGCTGAAGTTTTAACTCCTTTTTGGATCTCTGTCATTAAATTGAGTTCCTTTTGCTGAAATTTTCTGGGAAAGTTAACTCAAGTTTTCCATCAGTATTTCCAACGACTCAATGGAAAGTAAATCTCAAGCTTACATTTGAACTTGTATTTTTCCTTTTTCCTTTCAAAACTATTGTTACTTTCAGGTACACTCTAAAAATGTTTACTGACCCGATCACAGTGGTTTTGGGGTTCTTTAGGTTGAAATAAAAAAGCACGGTTACAAACTAAAGAATTTTTCAAATAATATCAATTGTTGTAGTTCAAATGAATAGTTTCTGGCCTTGCTGTCAGTTAACCATCTACATTGTTCTCTGTACTTGAACCAATAGGTCAAGCACAGAGCACTTATCATTGCGGGCGAAGATGACAGAATTATTCGCTATAAAGATGCAGTGGTGAGTTTCATTTTTAATCAAAAGAATCCATGTCTTCTGTTAAATTGCTTAGTTTTGCCATTTGTGATGTTAATGTATCTTTCCTCCATGCTTTTGATTGGGACTCCCTCGATCCTGAACCGTATGTGTTCATTGTTTGAACTGTTTTTTTTCCAACCAAATGTGCATATTTCTTGTGAGAGAAGAGTTTGATAGTGATTTTGTTTGGGTTTTTTGATTTTGCAGAGATTGCATTGTGAGCTACCAAATGCAATAATGCGAATAATAACAGATAGTGGCCATCTGCCCCATGTTGACAATCCTGCTTCTGTTTCAAAGCTTATTACAGATTTTGTGTTACATGATTCCTGCTAG

mRNA sequence

ATGACTCTGAAATGGTGCTTACCCTCACTGTCGCTTCACCAGCTTACGAGAACGCCAATGGCAGCGAAATGTGAAGGCCTTGGCCTTGGAGGAGTTTTATCCCATGGAAACTCTGGAGCATTCCCCTCGTTTCTTCCTAAAGAAGTCGGTTACTACCAAGCCCAAGCCTGCACAATGATTCTTTATACTTGTTCTTTTTTGCAGATGACATTATCTGAAACTCGCATTATGAGCAGCTGCGTTAAACCAGTAATAAGAAGTGAGAAGAATCCGGTGGTTCTACTCCATTGCTTTGACAGTTCTTGTTTGGAATGGAGTTATGCATTTCCTCTGCTTGAAGAGGCTGGTTTAGAAACGTGGGCTGTCGATGTTCTTGGATGGGGCTTTTCTGACTTGGATAGTCTTCCACCATGTAACGTCCAAACAAAGCGTTCTCATCTTTACCAGTTTTGGAAGACCTACATCAAGAGGCCAATGGTGTTAGTTGGACCAAGCCTTGGAGCAGCGGTTGCAGTTGATTTTGCAGTTAATCATCCGGAAGCTGTGGAAAAGCTGGTCTTGATCAATGCTTGTGTGTATAGTAAATGCACCGGGCACCCTACAAAGCTGCCGAAAGTGGTGGCATATGCTGGGGTTTCACTGTTAAAAAGTATCCCATTGCGCCTCATTGCAAACAAGTTAACATTCAATGGACTTTCACTTGCTCGAAATCTAGAATGGACGAATGTTGGCCGTTTGCACTGCCTATTGCCTTGGTGGGAAGATGCAACGGTTAATTTTATGAACAGCGGAGGATATAATGTCATTTCCCAGATTAAACAGGTCAAGCACAGAGCACTTATCATTGCGGGCGAAGATGACAGAATTATTCGCTATAAAGATGCAGTGAGATTGCATTGTGAGCTACCAAATGCAATAATGCGAATAATAACAGATAGTGGCCATCTGCCCCATGTTGACAATCCTGCTTCTGTTTCAAAGCTTATTACAGATTTTGTGTTACATGATTCCTGCTAG

Coding sequence (CDS)

ATGACTCTGAAATGGTGCTTACCCTCACTGTCGCTTCACCAGCTTACGAGAACGCCAATGGCAGCGAAATGTGAAGGCCTTGGCCTTGGAGGAGTTTTATCCCATGGAAACTCTGGAGCATTCCCCTCGTTTCTTCCTAAAGAAGTCGGTTACTACCAAGCCCAAGCCTGCACAATGATTCTTTATACTTGTTCTTTTTTGCAGATGACATTATCTGAAACTCGCATTATGAGCAGCTGCGTTAAACCAGTAATAAGAAGTGAGAAGAATCCGGTGGTTCTACTCCATTGCTTTGACAGTTCTTGTTTGGAATGGAGTTATGCATTTCCTCTGCTTGAAGAGGCTGGTTTAGAAACGTGGGCTGTCGATGTTCTTGGATGGGGCTTTTCTGACTTGGATAGTCTTCCACCATGTAACGTCCAAACAAAGCGTTCTCATCTTTACCAGTTTTGGAAGACCTACATCAAGAGGCCAATGGTGTTAGTTGGACCAAGCCTTGGAGCAGCGGTTGCAGTTGATTTTGCAGTTAATCATCCGGAAGCTGTGGAAAAGCTGGTCTTGATCAATGCTTGTGTGTATAGTAAATGCACCGGGCACCCTACAAAGCTGCCGAAAGTGGTGGCATATGCTGGGGTTTCACTGTTAAAAAGTATCCCATTGCGCCTCATTGCAAACAAGTTAACATTCAATGGACTTTCACTTGCTCGAAATCTAGAATGGACGAATGTTGGCCGTTTGCACTGCCTATTGCCTTGGTGGGAAGATGCAACGGTTAATTTTATGAACAGCGGAGGATATAATGTCATTTCCCAGATTAAACAGGTCAAGCACAGAGCACTTATCATTGCGGGCGAAGATGACAGAATTATTCGCTATAAAGATGCAGTGAGATTGCATTGTGAGCTACCAAATGCAATAATGCGAATAATAACAGATAGTGGCCATCTGCCCCATGTTGACAATCCTGCTTCTGTTTCAAAGCTTATTACAGATTTTGTGTTACATGATTCCTGCTAG

Protein sequence

MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSHGNSGAFPSFLPKEVGYYQAQACTMILYTCSFLQMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPEAVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEWTNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC
Homology
BLAST of Csor.00g070350 vs. ExPASy Swiss-Prot
Match: O05235 (Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yugF PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 5.0e-12
Identity = 65/250 (26.00%), Postives = 111/250 (44.40%), Query Frame = 0

Query: 89  KNPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTKRSHLY 148
           +  +V +H F SS   +    PLL +   +  A+D+  +G S+         Q     + 
Sbjct: 27  RQTLVCVHGFLSSAFSFRKVIPLLRDK-YDIIALDLPPFGQSEKSRTFIYTYQNLAKLVI 86

Query: 149 QFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPEAVEKLVLINACVYSKCTGHP-----TKL 208
              +    +  VLVG S+G  +++  A+  PE   K+VL+ +  Y K   HP     T +
Sbjct: 87  GILEHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLCSSGYLK-RSHPTIIFGTHI 146

Query: 209 PKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEWTNVGRLHCLLPWWEDATVNFMNS 268
           P    Y    L K   ++ + N    +  SL         GR       ++  T    + 
Sbjct: 147 PYFHLYIKRWLSKEGVMKNLLN--VVHDKSLIDEEMIDGYGRPFQDEQIFKAMTRFIRHR 206

Query: 269 GGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHVDNPA 328
            G     Q+K++   AL+I GE+DRI+  +   RLH +LPN+++  +  +GHL   + P 
Sbjct: 207 EGDLEPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHADLPNSVLYSLGQTGHLVPEERPE 266

Query: 329 SVSKLITDFV 334
            +S+ I DF+
Sbjct: 267 LISEHIADFI 272

BLAST of Csor.00g070350 vs. ExPASy Swiss-Prot
Match: B2JQW2 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) OX=391038 GN=mhpC PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.7e-10
Identity = 66/259 (25.48%), Postives = 106/259 (40.93%), Query Frame = 0

Query: 89  KNPVVLLHCFDSSCLEWSYAFPLLE---EAGLETWAVDVLGWGFSDLDSLPPCNVQTKRS 148
           K  +VLLH        W+  +  ++    AG     VD  GWG SD              
Sbjct: 36  KETLVLLHGSGPGASGWANFYRNVDAFANAGYRVILVDCPGWGKSDSIVCTGSRSDLNAR 95

Query: 149 HLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPEAVEKLVLINACVYSKCTGHPTKLPK 208
            L     T       LVG S+G   AV FA+++PE V KLVL+        TG P++   
Sbjct: 96  VLKGVLDTLGIERAHLVGNSMGGHSAVAFALSYPERVGKLVLMGG-----GTGGPSQFVP 155

Query: 209 VVAYAGVSLLKSI-------PLRLIANKLTFNGLSLARNLEWTN----VGRLHCLLPWWE 268
           +    G+ LL+ +        L+ + N   ++  ++   L  T     + R   L  + +
Sbjct: 156 MPT-EGIKLLQGLYRDPTLENLKKMLNVFVYDASTMTEELMQTRLDNMLARRDHLENFVK 215

Query: 269 DATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSG 328
             T N      Y    ++ ++K  AL+I G DDR +     +RL   LPNA   +    G
Sbjct: 216 SLTANPKQFPDYG--HRLNEIKAPALVIWGRDDRFVPLDVGLRLVWGLPNAEFHVFGRCG 275

Query: 329 HLPHVDNPASVSKLITDFV 334
           H    ++    ++++ DF+
Sbjct: 276 HWAQWEHAERFNQMLLDFL 286

BLAST of Csor.00g070350 vs. ExPASy Swiss-Prot
Match: A4JPX5 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Burkholderia vietnamiensis (strain G4 / LMG 22486) OX=269482 GN=mhpC PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 6.1e-10
Identity = 64/256 (25.00%), Postives = 107/256 (41.80%), Query Frame = 0

Query: 92  VVLLHCFDSSCLEWSYAFPLLE---EAGLETWAVDVLGWGFSDLDSLPPCNVQTKRSHLY 151
           +VLLH        W+     ++    AG     VD  GWG SD               L 
Sbjct: 39  LVLLHGSGPGATGWANFHRNVDAFANAGYRVILVDCPGWGKSDSIVCTGSRSDLNARVLA 98

Query: 152 QFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPEAVEKLVLINACVYSKCTGHPTKLPKVVA 211
               T       LVG S+G   AV FA+++PE V KLVL+        TG P++   +  
Sbjct: 99  GVLDTLGIGRAHLVGNSMGGHSAVAFALSYPERVGKLVLMGG-----GTGGPSQFVPMPT 158

Query: 212 YAGVSLLKSI-------PLRLIANKLTFNGLSLARNLEWTN----VGRLHCLLPWWEDAT 271
             G+ LL+++        L+ + N   ++  ++   L  T     +GR   L  + +  T
Sbjct: 159 -EGIKLLQALYRDPTLENLKKMLNVFVYDASTMTEELMQTRLENMLGRRDHLENFVKSLT 218

Query: 272 VNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLP 331
            N      Y    ++ ++K  AL+I G DDR +     +RL   +PNA + +    GH  
Sbjct: 219 ANPKQFPDYG--HRLSEIKAPALVIWGRDDRFVPMDVGLRLVWNMPNADLHVFGRCGHWA 278

Query: 332 HVDNPASVSKLITDFV 334
             ++    ++++ +F+
Sbjct: 279 QWEHAERFNRMVLEFL 286

BLAST of Csor.00g070350 vs. ExPASy Swiss-Prot
Match: P23974 (Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=menH PE=3 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 2.5e-08
Identity = 66/268 (24.63%), Postives = 112/268 (41.79%), Query Frame = 0

Query: 92  VVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTKR-SHLYQF 151
           VV LH F  S   W++   +L ++ L    +D LG G +D          T++ S L + 
Sbjct: 27  VVCLHGFTGSKQSWTFLDEMLPDSRL--IKIDCLGHGETDAPLNGKRYSTTRQVSDLAEI 86

Query: 152 WKTYIKRPMVLVGPSLGAAVAVDFAVNHPEAVEKLVLINACVYSKCTG-------HPTKL 211
           +       + L+G S+G  +A  FA+ +PE V  LVL +     K  G          KL
Sbjct: 87  FDQLKLHKVKLIGYSMGGRLAYSFAMTYPERVSALVLESTTPGLKTLGERRERIMRDRKL 146

Query: 212 PKVVAYAG----VSLLKSIPL-------------RLIANKLTFNGLSLARNLEWTNVGRL 271
              +   G    V+  ++IPL             R+ + +L  N + LA +L     G  
Sbjct: 147 ADFILRDGLEAFVAYWENIPLFSSQQRLAEDIRYRIRSGRLRNNKIGLANSLTGMGTGSQ 206

Query: 272 HCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGE-DDRIIRYKDAVRLHCELPNA 331
             L   W                S+++++    L+I GE D++       V  H  LP++
Sbjct: 207 PSL---W----------------SRVEEIDVPVLLICGEWDEKFCAINQEV--HKMLPSS 266

Query: 332 IMRIITDSGHLPHVDNPASVSKLITDFV 334
            + I+  +GH  HV+ P    K++++F+
Sbjct: 267 RIEIVPKAGHTVHVEQPRLFGKIVSEFL 271

BLAST of Csor.00g070350 vs. ExPASy Swiss-Prot
Match: P23133 (2-hydroxy-6-oxo-2,4-heptadienoate hydrolase OS=Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1) OX=351746 GN=todF PE=1 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 5.7e-08
Identity = 58/257 (22.57%), Postives = 104/257 (40.47%), Query Frame = 0

Query: 90  NPVVLLHCFDSSCLEWSYAFPLLEEAG--LETWAVDVLGWGFSDLDSLPPCNVQTKRSHL 149
           NPVVL+H        W+    ++ E        A D++G+GF+         V++  +HL
Sbjct: 28  NPVVLVHGSGPGVTAWANWRTVMPELSRHRRVIAPDMVGFGFTQRPHGIHYGVESWVAHL 87

Query: 150 YQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPEAVEKLVLINACVYS--------KCTGH 209
                      + LVG S G A+++ FA+  P  V +LVL+ A   S           G+
Sbjct: 88  AGILDALELDRVDLVGNSFGGALSLAFAIRFPHRVRRLVLMGAVGVSFELTDGLDAVWGY 147

Query: 210 PTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEWTNVG---RLHCLLPWWEDA 269
              +P        ++ K +        L  + L+  R    T  G       + P     
Sbjct: 148 EPSVP--------NMRKVMDYFAYDRSLVSDELAELRYKASTRPGFQEAFASMFPAPRQR 207

Query: 270 TVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHL 329
            V+ + S        I+ ++H  LI+ G DDR+I  + ++RL+  +  + + +    GH 
Sbjct: 208 WVDALASSD----QDIRDIRHETLILHGRDDRVIPLETSLRLNQLIEPSQLHVFGRCGHW 267

Query: 330 PHVDNPASVSKLITDFV 334
             ++      +L+ DF+
Sbjct: 268 VQIEQNRGFIRLVNDFL 272

BLAST of Csor.00g070350 vs. NCBI nr
Match: KAG6581698.1 (hypothetical protein SDJN03_21700, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 696 bits (1795), Expect = 6.51e-253
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSHGNSGAFPSFLPKEVGYYQAQACTMI 60
           MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSHGNSGAFPSFLPKEVGYYQAQACTMI
Sbjct: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSHGNSGAFPSFLPKEVGYYQAQACTMI 60

Query: 61  LYTCSFLQMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120
           LYTCSFLQMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW
Sbjct: 61  LYTCSFLQMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120

Query: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180
           AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE
Sbjct: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180

Query: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEW 240
           AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEW
Sbjct: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEW 240

Query: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300
           TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC
Sbjct: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300

Query: 301 ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC 338
           ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC
Sbjct: 301 ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC 338

BLAST of Csor.00g070350 vs. NCBI nr
Match: KAG7018179.1 (yugF [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 652 bits (1683), Expect = 7.71e-236
Identity = 321/338 (94.97%), Postives = 323/338 (95.56%), Query Frame = 0

Query: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSHGNSGAFPSFLPKEVGYYQAQACTMI 60
           MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSHGNSGAFPSFLPKEVG  +      +
Sbjct: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSHGNSGAFPSFLPKEVGKIKDPFARTM 60

Query: 61  LYTCSFLQMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120
                 L MTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW
Sbjct: 61  ARKIERLPMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120

Query: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180
           AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE
Sbjct: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180

Query: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEW 240
           AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEW
Sbjct: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEW 240

Query: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300
           TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC
Sbjct: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300

Query: 301 ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC 338
           ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC
Sbjct: 301 ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC 338

BLAST of Csor.00g070350 vs. NCBI nr
Match: XP_023513780.1 (uncharacterized protein LOC111778290 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 649 bits (1673), Expect = 2.58e-234
Identity = 319/338 (94.38%), Postives = 322/338 (95.27%), Query Frame = 0

Query: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSHGNSGAFPSFLPKEVGYYQAQACTMI 60
           MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLS GNSGAFPSFLPKEVG  +      +
Sbjct: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSDGNSGAFPSFLPKEVGKIKDPFARTM 60

Query: 61  LYTCSFLQMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120
                 L MTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW
Sbjct: 61  ARKIERLPMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120

Query: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180
           AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE
Sbjct: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180

Query: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEW 240
           AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRL+ANKLTFNGLSLARNLEW
Sbjct: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLVANKLTFNGLSLARNLEW 240

Query: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300
           TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC
Sbjct: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300

Query: 301 ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC 338
           ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC
Sbjct: 301 ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC 338

BLAST of Csor.00g070350 vs. NCBI nr
Match: XP_022955466.1 (uncharacterized protein LOC111457484 [Cucurbita moschata])

HSP 1 Score: 645 bits (1664), Expect = 6.07e-233
Identity = 318/338 (94.08%), Postives = 322/338 (95.27%), Query Frame = 0

Query: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSHGNSGAFPSFLPKEVGYYQAQACTMI 60
           MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLS GNSGAFPSFLPKEVG  +      +
Sbjct: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSDGNSGAFPSFLPKEVGKIKDPFARTM 60

Query: 61  LYTCSFLQMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120
                 L MTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW
Sbjct: 61  ARKIERLPMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120

Query: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180
           AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE
Sbjct: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180

Query: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEW 240
           AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTF+GLSLARNLEW
Sbjct: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFDGLSLARNLEW 240

Query: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300
           TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC
Sbjct: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300

Query: 301 ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC 338
           ELPNAIMRIIT+SGHLPHVDNPASVSKLITDFVLHDSC
Sbjct: 301 ELPNAIMRIITNSGHLPHVDNPASVSKLITDFVLHDSC 338

BLAST of Csor.00g070350 vs. NCBI nr
Match: XP_022980671.1 (uncharacterized protein LOC111479969 [Cucurbita maxima])

HSP 1 Score: 634 bits (1635), Expect = 1.54e-228
Identity = 313/338 (92.60%), Postives = 319/338 (94.38%), Query Frame = 0

Query: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSHGNSGAFPSFLPKEVGYYQAQACTMI 60
           MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLS GNSGAFPSFLPKEVG  +      +
Sbjct: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSDGNSGAFPSFLPKEVGKIKDPFARTM 60

Query: 61  LYTCSFLQMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120
                 L MTLSETRIMSSCVKPVIR +KNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW
Sbjct: 61  ASKIERLPMTLSETRIMSSCVKPVIR-KKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120

Query: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180
           AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE
Sbjct: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180

Query: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEW 240
           AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRL+ANKL FNGLSLARNL+W
Sbjct: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLVANKLIFNGLSLARNLDW 240

Query: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300
           TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRAL+IAGEDDRII YKDAVRLHC
Sbjct: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALVIAGEDDRIICYKDAVRLHC 300

Query: 301 ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC 338
           ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC
Sbjct: 301 ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC 337

BLAST of Csor.00g070350 vs. ExPASy TrEMBL
Match: A0A6J1GTU8 (uncharacterized protein LOC111457484 OS=Cucurbita moschata OX=3662 GN=LOC111457484 PE=4 SV=1)

HSP 1 Score: 645 bits (1664), Expect = 2.94e-233
Identity = 318/338 (94.08%), Postives = 322/338 (95.27%), Query Frame = 0

Query: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSHGNSGAFPSFLPKEVGYYQAQACTMI 60
           MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLS GNSGAFPSFLPKEVG  +      +
Sbjct: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSDGNSGAFPSFLPKEVGKIKDPFARTM 60

Query: 61  LYTCSFLQMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120
                 L MTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW
Sbjct: 61  ARKIERLPMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120

Query: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180
           AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE
Sbjct: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180

Query: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEW 240
           AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTF+GLSLARNLEW
Sbjct: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFDGLSLARNLEW 240

Query: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300
           TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC
Sbjct: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300

Query: 301 ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC 338
           ELPNAIMRIIT+SGHLPHVDNPASVSKLITDFVLHDSC
Sbjct: 301 ELPNAIMRIITNSGHLPHVDNPASVSKLITDFVLHDSC 338

BLAST of Csor.00g070350 vs. ExPASy TrEMBL
Match: A0A6J1IU82 (uncharacterized protein LOC111479969 OS=Cucurbita maxima OX=3661 GN=LOC111479969 PE=4 SV=1)

HSP 1 Score: 634 bits (1635), Expect = 7.45e-229
Identity = 313/338 (92.60%), Postives = 319/338 (94.38%), Query Frame = 0

Query: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSHGNSGAFPSFLPKEVGYYQAQACTMI 60
           MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLS GNSGAFPSFLPKEVG  +      +
Sbjct: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSDGNSGAFPSFLPKEVGKIKDPFARTM 60

Query: 61  LYTCSFLQMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120
                 L MTLSETRIMSSCVKPVIR +KNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW
Sbjct: 61  ASKIERLPMTLSETRIMSSCVKPVIR-KKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120

Query: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180
           AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE
Sbjct: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180

Query: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEW 240
           AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRL+ANKL FNGLSLARNL+W
Sbjct: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLVANKLIFNGLSLARNLDW 240

Query: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300
           TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRAL+IAGEDDRII YKDAVRLHC
Sbjct: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALVIAGEDDRIICYKDAVRLHC 300

Query: 301 ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC 338
           ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC
Sbjct: 301 ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC 337

BLAST of Csor.00g070350 vs. ExPASy TrEMBL
Match: A0A6J1K8F9 (uncharacterized protein LOC111492638 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492638 PE=4 SV=1)

HSP 1 Score: 602 bits (1553), Expect = 2.42e-216
Identity = 295/338 (87.28%), Postives = 310/338 (91.72%), Query Frame = 0

Query: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSHGNSGAFPSFLPKEVGYYQAQACTMI 60
           M LKW  PSL+LHQLTRTPM+AKCEGLG+  VLS GN G FPSFLPKEVG  +      +
Sbjct: 1   MILKWGFPSLALHQLTRTPMSAKCEGLGIVDVLSDGNCGGFPSFLPKEVGKIKDPFARTM 60

Query: 61  LYTCSFLQMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120
                 L +TLSETR+MSSCVKPVIRS+K+PVVLLHCFDSSCLEWSYAFPLLEEAGLETW
Sbjct: 61  ARKIERLPITLSETRLMSSCVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120

Query: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180
           AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWK+YIKRPMVLVGPSLGAAVA+DFAVNHPE
Sbjct: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPE 180

Query: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEW 240
           AVEKLVLINACVYS CTGHPTKLPKVVAYAGVSLLKSIPLRL+ANKLTFN LSLARNLEW
Sbjct: 181 AVEKLVLINACVYSNCTGHPTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNLEW 240

Query: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300
           TNVGRLHCLLPWWEDATVNFM SGGYNVIS IKQVKHRALIIAGEDDRIIRYKDAVRLHC
Sbjct: 241 TNVGRLHCLLPWWEDATVNFMYSGGYNVISLIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300

Query: 301 ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC 338
           ELPNAIMRIITDSGHLPHVD+PASVSKL+T+FV HDSC
Sbjct: 301 ELPNAIMRIITDSGHLPHVDSPASVSKLVTEFVFHDSC 338

BLAST of Csor.00g070350 vs. ExPASy TrEMBL
Match: A0A6J1KAV9 (uncharacterized protein LOC111492638 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492638 PE=4 SV=1)

HSP 1 Score: 602 bits (1553), Expect = 4.38e-216
Identity = 295/338 (87.28%), Postives = 310/338 (91.72%), Query Frame = 0

Query: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSHGNSGAFPSFLPKEVGYYQAQACTMI 60
           M LKW  PSL+LHQLTRTPM+AKCEGLG+  VLS GN G FPSFLPKEVG  +      +
Sbjct: 17  MILKWGFPSLALHQLTRTPMSAKCEGLGIVDVLSDGNCGGFPSFLPKEVGKIKDPFARTM 76

Query: 61  LYTCSFLQMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120
                 L +TLSETR+MSSCVKPVIRS+K+PVVLLHCFDSSCLEWSYAFPLLEEAGLETW
Sbjct: 77  ARKIERLPITLSETRLMSSCVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 136

Query: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180
           AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWK+YIKRPMVLVGPSLGAAVA+DFAVNHPE
Sbjct: 137 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPE 196

Query: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEW 240
           AVEKLVLINACVYS CTGHPTKLPKVVAYAGVSLLKSIPLRL+ANKLTFN LSLARNLEW
Sbjct: 197 AVEKLVLINACVYSNCTGHPTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNLEW 256

Query: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300
           TNVGRLHCLLPWWEDATVNFM SGGYNVIS IKQVKHRALIIAGEDDRIIRYKDAVRLHC
Sbjct: 257 TNVGRLHCLLPWWEDATVNFMYSGGYNVISLIKQVKHRALIIAGEDDRIIRYKDAVRLHC 316

Query: 301 ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC 338
           ELPNAIMRIITDSGHLPHVD+PASVSKL+T+FV HDSC
Sbjct: 317 ELPNAIMRIITDSGHLPHVDSPASVSKLVTEFVFHDSC 354

BLAST of Csor.00g070350 vs. ExPASy TrEMBL
Match: A0A6J1G926 (uncharacterized protein LOC111452078 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452078 PE=4 SV=1)

HSP 1 Score: 600 bits (1546), Expect = 2.82e-215
Identity = 291/338 (86.09%), Postives = 310/338 (91.72%), Query Frame = 0

Query: 1   MTLKWCLPSLSLHQLTRTPMAAKCEGLGLGGVLSHGNSGAFPSFLPKEVGYYQAQACTMI 60
           M LKW  PSL+LHQLTRTPM+AKCEGLG+G V S GN G FPSFLPKEVG  +      +
Sbjct: 1   MILKWGFPSLALHQLTRTPMSAKCEGLGIGDVFSDGNCGGFPSFLPKEVGKIKDPFARTM 60

Query: 61  LYTCSFLQMTLSETRIMSSCVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120
                 L +TLSETR+MSSCVKPVIRS+K+PVVLLHCFDSSCLEWSYAFPLLEEAGLETW
Sbjct: 61  ARKIERLPITLSETRLMSSCVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETW 120

Query: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPE 180
           AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWK+YIKRPMVLVGPSLGAAVA+DFAVNHPE
Sbjct: 121 AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPE 180

Query: 181 AVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSIPLRLIANKLTFNGLSLARNLEW 240
           AVEKLVLINACVYS CTGHPTKLPKVVAYAGVSLLKSIPLRL+ANKLTFN LSLARN+EW
Sbjct: 181 AVEKLVLINACVYSNCTGHPTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEW 240

Query: 241 TNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHC 300
           TNVGRLHCLLPWWEDATVNFM SGGYNV+S IKQVKHRALIIAGEDD+IIRYKDAVRLHC
Sbjct: 241 TNVGRLHCLLPWWEDATVNFMYSGGYNVVSLIKQVKHRALIIAGEDDKIIRYKDAVRLHC 300

Query: 301 ELPNAIMRIITDSGHLPHVDNPASVSKLITDFVLHDSC 338
           ELPNAIMRI+TDSGHLPHVD+PASVSKLIT+FV H+SC
Sbjct: 301 ELPNAIMRIVTDSGHLPHVDSPASVSKLITEFVFHNSC 338

BLAST of Csor.00g070350 vs. TAIR 10
Match: AT1G13820.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 379.8 bits (974), Expect = 2.3e-105
Identity = 184/295 (62.37%), Postives = 226/295 (76.61%), Query Frame = 0

Query: 41  FPSFLPKEVGYYQAQACTMILYTCSFLQMTLS--ETRIMSSCVKPVIRSEKNPVVLLHCF 100
           FP+FLP +V   +      +      L +++S  E  IMSSCV P++R+E  PVVLLH F
Sbjct: 32  FPTFLPTDVEKIKDTFALKLAARIERLPVSVSFREDSIMSSCVTPLMRNETTPVVLLHGF 91

Query: 101 DSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIKRP 160
           DSSCLEW Y +PLLEEAGLETWA D+LGWGFSDLD LPPC+V +KR H Y+FWK++IKRP
Sbjct: 92  DSSCLEWRYTYPLLEEAGLETWAFDILGWGFSDLDKLPPCDVASKREHFYKFWKSHIKRP 151

Query: 161 MVLVGPSLGAAVAVDFAVNHPEAVEKLVLINACVYSKCTGHPTKLPKVVAYAGVSLLKSI 220
           +VLVGPSLGAAVA+D AVNHPEAVE LVL++A VY++ TG+   LPK  AYAGV LLKSI
Sbjct: 152 VVLVGPSLGAAVAIDIAVNHPEAVESLVLMDASVYAEGTGNLATLPKAAAYAGVYLLKSI 211

Query: 221 PLRLIANKLTFNGLSLARNLEWTNVGRLHCLLPWWEDATVNFMNSGGYNVISQIKQVKHR 280
           PLRL  N + FNG+SL  + +WT +GRLHCL PWWEDATV+FM SGGYNV S IK+V  +
Sbjct: 212 PLRLYVNFICFNGISLETSWDWTKIGRLHCLYPWWEDATVSFMTSGGYNVTSLIKKVSQK 271

Query: 281 ALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHVDNPASVSKLITDFV 334
            LI+ GEDD+II  K A RLH EL NA ++ I++ GHLPHV+ PA+V+KLI +FV
Sbjct: 272 TLILWGEDDQIISNKLAWRLHGELSNARVKQISNCGHLPHVEKPAAVTKLIAEFV 326

BLAST of Csor.00g070350 vs. TAIR 10
Match: AT5G39220.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 373.2 bits (957), Expect = 2.1e-103
Identity = 176/302 (58.28%), Postives = 226/302 (74.83%), Query Frame = 0

Query: 37  NSGAFPSFLPKEV-----GYYQAQACTMILYTCSFLQMTLSETRIMSSCVKPVIR-SEKN 96
           N   FP+FLPKE+      + +A A  ++      LQM      +MSSC+KP+++  +K+
Sbjct: 24  NGDGFPTFLPKEIQNIKDPFARALAQRIVRIPVP-LQMGNFRGSVMSSCIKPLVQLHDKS 83

Query: 97  PVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTKRSHLYQF 156
           PVVLLHCFDSSCLEW   +PLLE+A LETWA+DVLGWGFSDL+ LPPC+  +KR HL++ 
Sbjct: 84  PVVLLHCFDSSCLEWRRTYPLLEQACLETWAIDVLGWGFSDLEKLPPCDAASKRHHLFEL 143

Query: 157 WKTYIKRPMVLVGPSLGAAVAVDFAVNHPEAVEKLVLINACVYSKCTGHPTKLPKVVAYA 216
           WKTYIKRPM+LVGPSLGA VAVDF   +PEAV+KLVLINA  YS+ TG   +LPK +AYA
Sbjct: 144 WKTYIKRPMILVGPSLGATVAVDFTATYPEAVDKLVLINANAYSEGTGRLKELPKSIAYA 203

Query: 217 GVSLLKSIPLRLIANKLTFNGLSLARNLEWTNVGRLHCLLPWWEDATVNFMNSGGYNVIS 276
           GV LLKS PLRL+AN L F    L+ N++WTN+GRLHC +PWWEDA V+FM SGGYNV S
Sbjct: 204 GVKLLKSFPLRLLANVLAFCSSPLSENIDWTNIGRLHCQMPWWEDAMVDFMISGGYNVAS 263

Query: 277 QIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHVDNPASVSKLIT 333
            IK + H+ L++  E+D+I+  + +V+L CEL NA++R + DSGHLPHV+NP  + KLI+
Sbjct: 264 HIKHIDHKTLVVCSENDQIVSNQLSVKLLCELANAVLREVPDSGHLPHVENPKHIVKLIS 323

BLAST of Csor.00g070350 vs. TAIR 10
Match: AT4G36530.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 70.9 bits (172), Expect = 2.3e-12
Identity = 38/107 (35.51%), Postives = 60/107 (56.07%), Query Frame = 0

Query: 84  VIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTK 143
           V++ E +P+VL+H F +S   W Y  P L +   + +A+D+LG+G+SD  +L   +    
Sbjct: 37  VVQGEGSPLVLIHGFGASVFHWRYNIPELAKK-YKVYALDLLGFGWSD-KALIEYDAMVW 96

Query: 144 RSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPEAVEKLVLINA 191
              +  F K  +K P V+VG SLG   A+  AV  PE V  + L+N+
Sbjct: 97  TDQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVGLPEQVTGVALLNS 141

BLAST of Csor.00g070350 vs. TAIR 10
Match: AT4G36530.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 70.9 bits (172), Expect = 2.3e-12
Identity = 38/107 (35.51%), Postives = 60/107 (56.07%), Query Frame = 0

Query: 84  VIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTK 143
           V++ E +P+VL+H F +S   W Y  P L +   + +A+D+LG+G+SD  +L   +    
Sbjct: 94  VVQGEGSPLVLIHGFGASVFHWRYNIPELAKK-YKVYALDLLGFGWSD-KALIEYDAMVW 153

Query: 144 RSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPEAVEKLVLINA 191
              +  F K  +K P V+VG SLG   A+  AV  PE V  + L+N+
Sbjct: 154 TDQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVGLPEQVTGVALLNS 198

BLAST of Csor.00g070350 vs. TAIR 10
Match: AT5G41900.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 50.8 bits (120), Expect = 2.4e-06
Identity = 43/137 (31.39%), Postives = 64/137 (46.72%), Query Frame = 0

Query: 64  CSFLQMTLSETRIMSSCVK----PVIRSEKNPVVLLHCFDSSCLEWSYA-FPLLEEAGLE 123
           CSF    L+ T   S  VK       +  ++ VV +H F SS   W+   FP   ++   
Sbjct: 161 CSFCTSWLTSTNRDSLFVKVQQPKDNKKARDNVVFIHGFVSSSAFWTETLFPNFSDSAKS 220

Query: 124 TW---AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKTYIK----RPMVLVGPSLGAAVA 183
            +   AVD+LG+G S     P  ++ T R HL    K+ I     +   +V  SLG  +A
Sbjct: 221 NYRFIAVDLLGYGRS---PKPNDSLYTLREHLEMIEKSVISKFKLKTFHIVAHSLGCILA 280

Query: 184 VDFAVNHPEAVEKLVLI 189
           +  AV HP A++ L L+
Sbjct: 281 LALAVKHPGAIKSLTLL 294

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O052355.0e-1226.00Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yu... [more]
B2JQW22.7e-1025.482-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Para... [more]
A4JPX56.1e-1025.002-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Burk... [more]
P239742.5e-0824.63Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase OS=Bacil... [more]
P231335.7e-0822.572-hydroxy-6-oxo-2,4-heptadienoate hydrolase OS=Pseudomonas putida (strain ATCC 7... [more]
Match NameE-valueIdentityDescription
KAG6581698.16.51e-253100.00hypothetical protein SDJN03_21700, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7018179.17.71e-23694.97yugF [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023513780.12.58e-23494.38uncharacterized protein LOC111778290 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022955466.16.07e-23394.08uncharacterized protein LOC111457484 [Cucurbita moschata][more]
XP_022980671.11.54e-22892.60uncharacterized protein LOC111479969 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GTU82.94e-23394.08uncharacterized protein LOC111457484 OS=Cucurbita moschata OX=3662 GN=LOC1114574... [more]
A0A6J1IU827.45e-22992.60uncharacterized protein LOC111479969 OS=Cucurbita maxima OX=3661 GN=LOC111479969... [more]
A0A6J1K8F92.42e-21687.28uncharacterized protein LOC111492638 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1KAV94.38e-21687.28uncharacterized protein LOC111492638 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1G9262.82e-21586.09uncharacterized protein LOC111452078 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT1G13820.12.3e-10562.37alpha/beta-Hydrolases superfamily protein [more]
AT5G39220.12.1e-10358.28alpha/beta-Hydrolases superfamily protein [more]
AT4G36530.12.3e-1235.51alpha/beta-Hydrolases superfamily protein [more]
AT4G36530.22.3e-1235.51alpha/beta-Hydrolases superfamily protein [more]
AT5G41900.12.4e-0631.39alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 96..114
score: 27.99
coord: 175..188
score: 28.57
coord: 116..131
score: 35.8
coord: 310..332
score: 25.1
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 279..293
score: 35.63
coord: 161..174
score: 43.1
coord: 116..131
score: 34.05
coord: 175..188
score: 41.87
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 92..325
e-value: 3.9E-12
score: 47.2
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 81..336
e-value: 3.7E-36
score: 127.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 88..333
NoneNo IPR availablePANTHERPTHR43689:SF37ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 17..335
NoneNo IPR availablePANTHERPTHR43689HYDROLASEcoord: 17..335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g070350.m01Csor.00g070350.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity