Csor.00g070150 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g070150
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionisoflavone reductase homolog
LocationCsor_Chr19: 1493511 .. 1496798 (+)
RNA-Seq ExpressionCsor.00g070150
SyntenyCsor.00g070150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGTTCTTGTGGTGGGTGGGACTGGTTATTTGGGGAAGAGGATCGTCAAGGCCAGCCTTTTGGAGGGGCACCAGACGTATGTGGTTCAACGGCCTGAGATTGGGCTCGACATTGAGAAGCTTCAGCTCCTCCTCTCCTTTAAGAGACAGGGGGCCGTGCTCGTGCCCGCCTCTTTCTCGGACTTTCAGAGCCTTGTCGACGCGGTGAAGCGAGTCGACGTCGTGATATCGGCCATGTCCGGTGTCCATTTCAGGTCACATAACCTTCTCCTCCAACTCAAGCTTGTTGAAGCCATTCAAGTTGCTGGCAATGTCGAGGTTTTTTTTCTTACCCTCTTCCACTTTTCTATTCATATTCATATTTAGGATGGAAATTTCTAGGACACCGAGCGATGTACTAACGAGGACGCTCGCCCCCAAGGGAGTGGATTGTGAGATCCCACATCAGTTGGAGAGGGGAATGAAGCATTCCTTATAAGGGGTGTGGAAACCTCTCTTTTGTAGACCTAGACGCGTTTTAAAACCGTGAGACTGATAGTGATACGTAACGGGTCAAACCGAACAATATCTACTAGCAATTGACTTTGGCAGTTACAAATGGTATCAGAGCTAGATACTAGGGGTACCAACGAGGATGCTGGGACTCCCAAGGGGTGGATTATGAGATCCCATATCAGTTAAAGAAGGGAATGAAACATTCATTATAAGGGTGTGAAAACCTCAACCTAGTAGACCTAGACGCGTTTTAAAATCGTGAGGTTGATGACGATATGTAACGGGCAAAACTGGACAATATCTACTATCAGCTGACTCTGGCTATTATAAATGGTATCAGAGCTGACACTAGACGGAGTACCAGTAAGGATGCTGGGATCTAAAGGGGTGGATTGTGAGATCTCACATCGGTTGGAGAGGAGAACGAAACATTCCTTATAAGGGTGTGGAGGTTGGAGAGGAGAACGAAACATTTCTTATAAGGGTGTGGAAACTTCAACCTAGTAAACGACACGTTTTAAAACCGTAAGGTTAACAATGATATATAACGGAACATAGCATACAATATCGGCTTGCGGTGGGCTTGGGCTGTTAGAGTTACAACTCAATCCAACTTTCATCCTATTTTTCAAAAGATTAAGAATATTTAGTTATGAACGTAACATTTATTCAACTAGCTGGGTTGTGAACTATATTTGGATTGATCGGGACACCAATCCACCCAACCTGAAATTTTAGATTTGTCCAAAAAAAAATATTTATAACTGAACACTAGTCTCCAAAAAAAAAAATTCTCGAGACTCAATCTGCTTTATCTTTTAGGTTGATCAATAATTTAACATAAACGAACCGACTAATTTATTGAAATTCTCGATATAGAGATTCTTACCATCAGAGTATGGAATAGACCCAGCAAGAATGAGAGATGCACTCGAACCGGGAAGAGAAACATTTGATCAAAAAATGACCATAAGAAAAGCAATAGAGGATGCCAACATTCCTTTCACCTACGTCTCAGCCAACTGCTTTGCCGGTTACTTTGCCGGTAGCCTGTCTCAGATGCAAAGTCTTGTTCCTCCCACTCATCAAGTTGCCCTTTATGGAGATGGCAACGTTAAAGGTAACCCACCACCAATTATTCTTAATACAAACTCGACGCTACTCTATTTATTTTCCAATACTAGGTGTCGGTGAAGTCAGTGGTCAAACTTTTAACGTTTTGATCGATGATATATTGTTAAAGAATAATGTTGTTTAAATATAGAGATGTTAATTTAATTCACGAGTCTCATCATATATAGAGTGAAAAATCTCCGTTTAAATGGAGAATGGGAGAGAGACCAGAGAGAGATTTCTCTCTATTAGCTAAACGGGTATTTCACGTCGCCATTCCCACCCTACCCACCCCAATCCCTACAAATTCCAAGGGGAGAATCTCGATTTCAGTCTCTGTAAATATAAGTGAGAAATTTTATAAGAACAAGGATTGATCTCATCCCCACTCCATTCCGTAGACATCTTAATTGAAATATACAAAATTTTATGGCAAAATTTACAGTGGTATACGTGGACGAAGATGACATTGCAACGTACATCGTGAAGGCAGCAACAGATCCTCGAACTATGAACAAAACAGTGTATATTAGGCCTCCAGACAACATTCTCTCCCAAAGAGAACTGATTCAGAAATGGGAAGCACTTTCTGGAAAAGTGTTAGAAAAGAACAGTGTTTCTGCTCAAGATTTTCTTGCAAGGATGAAAGGTAAAGTTTTATATAATATATATAACACTCAAATTTTATATATAATGAAAATGAAAATGGATGTCCCATCTCGGTTGGAAAGGAGAACGAAACACCTTTTGTAAGGGGTGTGGAAACCTTTCCCTAACACAGGCATTTTAAAAAACCTTGAGAGAAAGCCTAAACAGAACAATATCTATTAGCGGTGGGCCTGAGCTATTACAAATGGTATCAGAGCCAGACACCAAACGATTGCCAGCGAGGAGGCTGTTCCCTGAAGGGGACAGACACGAGGCGGTGTGTCTGTAAGGATGTTGGACCCCGAAGGGGGTGGATTGTGATGTCCCACATCGTTTGGGGAGGAGAGCGAAACATCCTTTATAAGGGTGTGGAAACCTCTCCCATTAAGGATGCTGGGCTCCGAAGGAGGGTGGATTGTGATGTCCCACATCCGTTGGGGAGGAGAGCGAAACACCCTTTATAAGGGTGTGGAAACCTCTCCTAATAAGAATGTTGGGCCCCGAAAAAGAGTGGATTGTGATGTCTCACATTGGTTGAGGAGGAGAGCGTAACACCCTTTATAAGGGTGTGGAAACCTCTCCCAGTAAAGATGCTGGACCTCGAAGGGTCTCCCAGTAAGGATGTTGGGCCCCGAAAGGGGTGGATTGTGATGTCCCACATCGGTTGAGGAGGAGAGCGTAACACCCTTTATAAGGGTGTGGAAACCTCTCCCAGTAAAGATGCTGGACCCGAGGAGGGGAGCGTAACACTCTTTATAAGGGTGTGAAAACCTCTCCCTAACAGAAATGTTTTAAAAACCTCGAAGAAAAGTCTAACGGTAGGCCTGTATTCAAAAAAATAAAAATAAGTATAAAATTGATGCAGATCTGGACATAGCACAGCAGGCTGGAGTGGGTCATTTCTATCACATATTCTTTGAAGGTTGTTTGACAAATTTTGAAATAGGAGAAGGAGAAGAAGAAGCTTCAAAGCTTTATCCAGAAGTGAACTACACTCGCATGGATGACTATCTCAAGATTTTCTTATGA

mRNA sequence

ATGAAGGTTCTTGTGGTGGGTGGGACTGGTTATTTGGGGAAGAGGATCGTCAAGGCCAGCCTTTTGGAGGGGCACCAGACGTATGTGGTTCAACGGCCTGAGATTGGGCTCGACATTGAGAAGCTTCAGCTCCTCCTCTCCTTTAAGAGACAGGGGGCCGTGCTCGTGCCCGCCTCTTTCTCGGACTTTCAGAGCCTTGTCGACGCGGTGAAGCGAGTCGACGTCGTGATATCGGCCATGTCCGGTGTCCATTTCAGGTCACATAACCTTCTCCTCCAACTCAAGCTTGTTGAAGCCATTCAAGTTGCTGGCAATGTCGAGAGATTCTTACCATCAGAGTATGGAATAGACCCAGCAAGAATGAGAGATGCACTCGAACCGGGAAGAGAAACATTTGATCAAAAAATGACCATAAGAAAAGCAATAGAGGATGCCAACATTCCTTTCACCTACGTCTCAGCCAACTGCTTTGCCGGTTACTTTGCCGGTAGCCTGTCTCAGATGCAAAGTCTTGTTCCTCCCACTCATCAAGTTGCCCTTTATGGAGATGGCAACGTTAAAGTGGTATACGTGGACGAAGATGACATTGCAACGTACATCGTGAAGGCAGCAACAGATCCTCGAACTATGAACAAAACAGTGTATATTAGGCCTCCAGACAACATTCTCTCCCAAAGAGAACTGATTCAGAAATGGGAAGCACTTTCTGGAAAAGTGTTAGAAAAGAACAGTGTTTCTGCTCAAGATTTTCTTGCAAGGATGAAAGATCTGGACATAGCACAGCAGGCTGGAGTGGGTCATTTCTATCACATATTCTTTGAAGGTTGTTTGACAAATTTTGAAATAGGAGAAGGAGAAGAAGAAGCTTCAAAGCTTTATCCAGAAGTGAACTACACTCGCATGGATGACTATCTCAAGATTTTCTTATGA

Coding sequence (CDS)

ATGAAGGTTCTTGTGGTGGGTGGGACTGGTTATTTGGGGAAGAGGATCGTCAAGGCCAGCCTTTTGGAGGGGCACCAGACGTATGTGGTTCAACGGCCTGAGATTGGGCTCGACATTGAGAAGCTTCAGCTCCTCCTCTCCTTTAAGAGACAGGGGGCCGTGCTCGTGCCCGCCTCTTTCTCGGACTTTCAGAGCCTTGTCGACGCGGTGAAGCGAGTCGACGTCGTGATATCGGCCATGTCCGGTGTCCATTTCAGGTCACATAACCTTCTCCTCCAACTCAAGCTTGTTGAAGCCATTCAAGTTGCTGGCAATGTCGAGAGATTCTTACCATCAGAGTATGGAATAGACCCAGCAAGAATGAGAGATGCACTCGAACCGGGAAGAGAAACATTTGATCAAAAAATGACCATAAGAAAAGCAATAGAGGATGCCAACATTCCTTTCACCTACGTCTCAGCCAACTGCTTTGCCGGTTACTTTGCCGGTAGCCTGTCTCAGATGCAAAGTCTTGTTCCTCCCACTCATCAAGTTGCCCTTTATGGAGATGGCAACGTTAAAGTGGTATACGTGGACGAAGATGACATTGCAACGTACATCGTGAAGGCAGCAACAGATCCTCGAACTATGAACAAAACAGTGTATATTAGGCCTCCAGACAACATTCTCTCCCAAAGAGAACTGATTCAGAAATGGGAAGCACTTTCTGGAAAAGTGTTAGAAAAGAACAGTGTTTCTGCTCAAGATTTTCTTGCAAGGATGAAAGATCTGGACATAGCACAGCAGGCTGGAGTGGGTCATTTCTATCACATATTCTTTGAAGGTTGTTTGACAAATTTTGAAATAGGAGAAGGAGAAGAAGAAGCTTCAAAGCTTTATCCAGAAGTGAACTACACTCGCATGGATGACTATCTCAAGATTTTCTTATGA

Protein sequence

MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFSDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPARMRDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVALYGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVLEKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTRMDDYLKIFL
Homology
BLAST of Csor.00g070150 vs. ExPASy Swiss-Prot
Match: P52581 (Isoflavone reductase homolog OS=Lupinus albus OX=3870 PE=2 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 7.7e-137
Identity = 232/308 (75.32%), Postives = 274/308 (88.96%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           KVLVVGGTGY+G+RIVKASL  GH+T+++QRPEIGLDIEKLQ+LLSFK+QGA+LV ASFS
Sbjct: 5   KVLVVGGTGYVGRRIVKASLEHGHETFILQRPEIGLDIEKLQILLSFKKQGAILVEASFS 64

Query: 62  DFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPARM 121
           D +SLVDAVK VDVVI  MSGVHFRSHNLL QLKLVEAI+ AGN++RFLPSE+G+DPA M
Sbjct: 65  DHKSLVDAVKLVDVVICTMSGVHFRSHNLLTQLKLVEAIKDAGNIKRFLPSEFGMDPALM 124

Query: 122 RDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVALY 181
             ALEPGR TFD+KMT+RKAIE+ANIPFTY+SANCFAGYFAG+LSQM++L+PP  +V LY
Sbjct: 125 GHALEPGRVTFDEKMTVRKAIEEANIPFTYISANCFAGYFAGNLSQMKTLLPPRDKVLLY 184

Query: 182 GDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVLE 241
           GDGNVK VY+DEDD+ATY +K   DPRT+NKTVY+RPP+NIL+ +ELI+KWE L GK LE
Sbjct: 185 GDGNVKPVYMDEDDVATYTIKTIDDPRTLNKTVYLRPPENILTHKELIEKWEELIGKQLE 244

Query: 242 KNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTRM 301
           KNS+S +DFL+ +K LD A Q GVGHFYHIF+EGCLTNFEIGE  EEAS+LYPEVNYTRM
Sbjct: 245 KNSISEKDFLSTLKGLDFASQVGVGHFYHIFYEGCLTNFEIGENGEEASELYPEVNYTRM 304

Query: 302 DDYLKIFL 310
           D YLK+++
Sbjct: 305 DQYLKVYV 312

BLAST of Csor.00g070150 vs. ExPASy Swiss-Prot
Match: A3R052 (Bifunctional pinoresinol-lariciresinol reductase OS=Linum perenne OX=35941 GN=PLR_Lp1 PE=1 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 7.2e-127
Identity = 222/309 (71.84%), Postives = 261/309 (84.47%), Query Frame = 0

Query: 3   VLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFSD 62
           VLVVGGTGY+GKRIV ASL  GH TYV++RP  GLDIEKLQLLLSFK++GA LV ASFSD
Sbjct: 6   VLVVGGTGYIGKRIVSASLYLGHDTYVLKRPGTGLDIEKLQLLLSFKKRGAHLVEASFSD 65

Query: 63  FQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPARMR 122
             SLV AV+ VDVVI  MSGVHFRSHN+LLQLKLVEAI+ AGNV+RF+PSE+G+DPARM 
Sbjct: 66  HDSLVRAVRLVDVVICTMSGVHFRSHNILLQLKLVEAIKEAGNVKRFIPSEFGMDPARMG 125

Query: 123 DALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVALYG 182
            A+EPGRETFDQKM +RKAIE+ANIP TY+SANCFAGYF G+LSQ+ +L PP+ +V +YG
Sbjct: 126 QAMEPGRETFDQKMVVRKAIEEANIPHTYISANCFAGYFVGNLSQLGTLTPPSDKVIIYG 185

Query: 183 DGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVLEK 242
           DGNVKVVYVDEDD+A Y +KA  D RT+NKTVY+RPP+N++SQREL+  WE LSG  LEK
Sbjct: 186 DGNVKVVYVDEDDVAKYTIKAIEDDRTVNKTVYLRPPENMMSQRELVAVWEKLSGNQLEK 245

Query: 243 NSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIG--EGEEEASKLYPEVNYTR 302
             +  QDFLA M+   +A+QAG+GHFYHIF+EGCLTNFEI    GEEEAS+LYPEV YTR
Sbjct: 246 IELPPQDFLALMEGTTVAEQAGIGHFYHIFYEGCLTNFEINAENGEEEASRLYPEVEYTR 305

Query: 303 MDDYLKIFL 310
           + DYLKI+L
Sbjct: 306 VHDYLKIYL 314

BLAST of Csor.00g070150 vs. ExPASy Swiss-Prot
Match: Q9FVQ6 (Pinoresinol reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PRR1 PE=1 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 1.7e-123
Identity = 214/308 (69.48%), Postives = 259/308 (84.09%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           +VLVVG TGY+GKRIV+A L EGH+TYV+QRPEIGL+IEK+QL LSFK+ GA +V  SFS
Sbjct: 11  RVLVVGATGYIGKRIVRACLAEGHETYVLQRPEIGLEIEKVQLFLSFKKLGARIVEGSFS 70

Query: 62  DFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPARM 121
           D QSLV AVK VDVV+SAMSGVHFRSHN+L+QLKLVEAI+ AGNV+RFLPSE+G+DP RM
Sbjct: 71  DHQSLVSAVKLVDVVVSAMSGVHFRSHNILVQLKLVEAIKEAGNVKRFLPSEFGMDPPRM 130

Query: 122 RDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVALY 181
             AL PGRETFDQKM +R+AIE A IP+TYV   CFA YFAG+LSQM +L+PP  +V +Y
Sbjct: 131 GHALPPGRETFDQKMEVRQAIEAAGIPYTYVVGACFAAYFAGNLSQMVTLLPPKEKVNIY 190

Query: 182 GDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVLE 241
           GDGNVKVV+ DEDDIA Y  K   DPRT+NKTV IRPPDN+L+Q EL+Q WE L+GK LE
Sbjct: 191 GDGNVKVVFADEDDIAKYTAKTLNDPRTLNKTVNIRPPDNVLTQLELVQIWEKLTGKELE 250

Query: 242 KNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTRM 301
           K +++AQDFLA ++ ++I  QAG+GHFYHIF+EGCLT+ E+GE +EEAS LYP+V Y RM
Sbjct: 251 KTNIAAQDFLANIEQMEIPHQAGIGHFYHIFYEGCLTDHEVGE-DEEASSLYPDVKYKRM 310

Query: 302 DDYLKIFL 310
           DDYL++FL
Sbjct: 311 DDYLRMFL 317

BLAST of Csor.00g070150 vs. ExPASy Swiss-Prot
Match: P0DKC8 (Bifunctional pinoresinol-lariciresinol reductase OS=Linum usitatissimum OX=4006 PE=2 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 1.6e-118
Identity = 208/310 (67.10%), Postives = 253/310 (81.61%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           +VLVVGGTGY+GKRIVKAS+  GH TYV++RPE GLDIEK QLLLSFK+QGA LV ASFS
Sbjct: 5   RVLVVGGTGYIGKRIVKASIEHGHDTYVLKRPETGLDIEKFQLLLSFKKQGAHLVEASFS 64

Query: 62  DFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPARM 121
           D +SLV AVK VDVVI  +SG H RS  LLLQLKLVEAI+ AGNV+RF+PSE+G+DPARM
Sbjct: 65  DHESLVRAVKLVDVVICTVSGAHSRS--LLLQLKLVEAIKEAGNVKRFIPSEFGMDPARM 124

Query: 122 RDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVALY 181
            DALEPGRETFD KM +RKAIEDANIP TY+SANCF GYF G+LSQ+  L PP+ +V +Y
Sbjct: 125 GDALEPGRETFDLKMVVRKAIEDANIPHTYISANCFGGYFVGNLSQLGPLTPPSDKVTIY 184

Query: 182 GDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVLE 241
           GDGNVKVVY+DEDD+ATY +    D RT+NKT+Y RPP+N+++ R+L++ WE LSG  L+
Sbjct: 185 GDGNVKVVYMDEDDVATYTIMTIEDDRTLNKTMYFRPPENVITHRQLVETWEKLSGNQLQ 244

Query: 242 KNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEE--EASKLYPEVNYT 301
           K  +S+QDFLA M+  D+A+Q  +GH YHI++EGCLTNF+I   ++  EAS LYPEV YT
Sbjct: 245 KTELSSQDFLALMEGKDVAEQIVIGHLYHIYYEGCLTNFDIDADQDQVEASSLYPEVEYT 304

Query: 302 RMDDYLKIFL 310
           RM DYL I+L
Sbjct: 305 RMKDYLMIYL 312

BLAST of Csor.00g070150 vs. ExPASy Swiss-Prot
Match: Q4R0H9 (Bifunctional pinoresinol-lariciresinol reductase 1 OS=Linum usitatissimum OX=4006 GN=PLR_Lu1 PE=1 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 2.8e-118
Identity = 207/310 (66.77%), Postives = 253/310 (81.61%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           +VLVVGGTGY+GKRIVKAS+  GH TYV++RPE GLDIEK QLLLSFK+QGA LV ASFS
Sbjct: 5   RVLVVGGTGYIGKRIVKASIEHGHDTYVLKRPETGLDIEKFQLLLSFKKQGAHLVEASFS 64

Query: 62  DFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPARM 121
           D +SLV AVK VDVVI  +SG H RS  LLLQLKLVEAI+ AGNV+RF+PSE+G+DPARM
Sbjct: 65  DHESLVRAVKLVDVVICTVSGAHSRS--LLLQLKLVEAIKEAGNVKRFIPSEFGMDPARM 124

Query: 122 RDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVALY 181
            DALEPGRETFD KM +RKAIEDANIP TY+SANCF GYF G+LSQ+  L PP+ +V +Y
Sbjct: 125 GDALEPGRETFDLKMVVRKAIEDANIPHTYISANCFGGYFVGNLSQLGPLTPPSDKVTIY 184

Query: 182 GDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVLE 241
           GDGNVKVVY+DEDD+ATY +    D RT+NKT+Y+RPP+N+++ R+L++ WE LSG  L+
Sbjct: 185 GDGNVKVVYMDEDDVATYTIMTIEDDRTLNKTMYLRPPENVITHRQLVETWEKLSGNQLQ 244

Query: 242 KNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEE--EASKLYPEVNYT 301
           K  +S+QDFLA M+  D+A+Q  +GH YHI++EGCLTNF+I   ++  EAS LYPEV Y 
Sbjct: 245 KTELSSQDFLALMEGKDVAEQVVIGHLYHIYYEGCLTNFDIDAAQDQVEASSLYPEVEYI 304

Query: 302 RMDDYLKIFL 310
           RM DYL I+L
Sbjct: 305 RMKDYLMIYL 312

BLAST of Csor.00g070150 vs. NCBI nr
Match: KAG6571626.1 (hypothetical protein SDJN03_28354, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 610 bits (1574), Expect = 3.34e-220
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPAR 120
           SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPAR
Sbjct: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVAL 180
           MRDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVAL
Sbjct: 121 MRDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVAL 180

Query: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240
           YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL
Sbjct: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240

Query: 241 EKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300
           EKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR
Sbjct: 241 EKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300

Query: 301 MDDYLKIFL 309
           MDDYLKIFL
Sbjct: 301 MDDYLKIFL 309

BLAST of Csor.00g070150 vs. NCBI nr
Match: XP_022967298.1 (isoflavone reductase homolog [Cucurbita maxima])

HSP 1 Score: 602 bits (1552), Expect = 7.53e-217
Identity = 303/309 (98.06%), Postives = 307/309 (99.35%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPAR 120
           SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNV+RFLPSEYGIDPAR
Sbjct: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVAL 180
           MRDALEPGRETFDQKMTIRKAIE+ANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQV L
Sbjct: 121 MRDALEPGRETFDQKMTIRKAIEEANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVTL 180

Query: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240
           YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL
Sbjct: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240

Query: 241 EKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300
           EKN VSAQDFLARMKDLDIAQQAGVGHFYH+FFEGCLTNFEIGEGEEEAS+LYPEVNYTR
Sbjct: 241 EKNRVSAQDFLARMKDLDIAQQAGVGHFYHLFFEGCLTNFEIGEGEEEASRLYPEVNYTR 300

Query: 301 MDDYLKIFL 309
           MDDYLKIFL
Sbjct: 301 MDDYLKIFL 309

BLAST of Csor.00g070150 vs. NCBI nr
Match: XP_023511514.1 (isoflavone reductase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 591 bits (1523), Expect = 1.98e-212
Identity = 298/309 (96.44%), Postives = 301/309 (97.41%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPAR 120
           SDF SLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNV+RFLPSEYGIDPAR
Sbjct: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVAL 180
           MRDALEPGRETFDQKM IRKAIE+ANIPFTYVSANCFAGYFAGSLSQM +LVPPTHQV L
Sbjct: 121 MRDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTL 180

Query: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240
           YGDGNVK VYVDEDDIATYIVKAATDPRTMNKTVYIRPP NILSQRELIQKWEALSGKVL
Sbjct: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVL 240

Query: 241 EKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300
           EKN VSAQDFL RMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR
Sbjct: 241 EKNRVSAQDFLTRMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300

Query: 301 MDDYLKIFL 309
           MDDYLKIFL
Sbjct: 301 MDDYLKIFL 309

BLAST of Csor.00g070150 vs. NCBI nr
Match: XP_022963989.1 (isoflavone reductase homolog [Cucurbita moschata])

HSP 1 Score: 587 bits (1513), Expect = 6.63e-211
Identity = 296/309 (95.79%), Postives = 300/309 (97.09%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPAR 120
           SDF SLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNV+RFLPSEYGIDPAR
Sbjct: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVAL 180
           MRDALEPGRETFDQKM +RKAIE+ANIPFTYVSANCFAGYFAGSLSQM +L PPTHQV L
Sbjct: 121 MRDALEPGRETFDQKMIVRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLAPPTHQVTL 180

Query: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240
           YGDGNVK VYVDEDDIATYIVKAATDPRTMNKTVYIRP DNILSQRELIQKWEALS KVL
Sbjct: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPRDNILSQRELIQKWEALSEKVL 240

Query: 241 EKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300
           EKNSVSAQDFL RMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR
Sbjct: 241 EKNSVSAQDFLTRMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300

Query: 301 MDDYLKIFL 309
           MDDYLKIFL
Sbjct: 301 MDDYLKIFL 309

BLAST of Csor.00g070150 vs. NCBI nr
Match: XP_038887505.1 (isoflavone reductase homolog [Benincasa hispida])

HSP 1 Score: 572 bits (1474), Expect = 1.05e-204
Identity = 283/309 (91.59%), Postives = 300/309 (97.09%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           M+VLVVGGTGYLGKRIVKASLLEGHQT+V+QRPEIGLDIEKLQLLLSFK+QGAVLVPASF
Sbjct: 17  MRVLVVGGTGYLGKRIVKASLLEGHQTFVIQRPEIGLDIEKLQLLLSFKKQGAVLVPASF 76

Query: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPAR 120
           SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGN++R +PSEYGIDPAR
Sbjct: 77  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNIKRLVPSEYGIDPAR 136

Query: 121 MRDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVAL 180
           M +ALEPGRETFDQKM IRKAIE+ANIPFTY+SANCFAGYFAG+LSQM +LVPPTHQV L
Sbjct: 137 MGNALEPGRETFDQKMVIRKAIEEANIPFTYISANCFAGYFAGNLSQMLTLVPPTHQVTL 196

Query: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240
           YGDGNVKV+YVDEDDIATYIVKAATDPRT NKTVYIRPP NILSQRELI+KWE LSGKVL
Sbjct: 197 YGDGNVKVIYVDEDDIATYIVKAATDPRTENKTVYIRPPQNILSQRELIEKWEKLSGKVL 256

Query: 241 EKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300
           EK SVSAQDFLARMKD+DIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKL+PEVNYTR
Sbjct: 257 EKKSVSAQDFLARMKDMDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLFPEVNYTR 316

Query: 301 MDDYLKIFL 309
           MDDYLKIF+
Sbjct: 317 MDDYLKIFI 325

BLAST of Csor.00g070150 vs. ExPASy TrEMBL
Match: A0A6J1HUP1 (isoflavone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111466861 PE=4 SV=1)

HSP 1 Score: 602 bits (1552), Expect = 3.65e-217
Identity = 303/309 (98.06%), Postives = 307/309 (99.35%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPAR 120
           SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNV+RFLPSEYGIDPAR
Sbjct: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVAL 180
           MRDALEPGRETFDQKMTIRKAIE+ANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQV L
Sbjct: 121 MRDALEPGRETFDQKMTIRKAIEEANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVTL 180

Query: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240
           YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL
Sbjct: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240

Query: 241 EKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300
           EKN VSAQDFLARMKDLDIAQQAGVGHFYH+FFEGCLTNFEIGEGEEEAS+LYPEVNYTR
Sbjct: 241 EKNRVSAQDFLARMKDLDIAQQAGVGHFYHLFFEGCLTNFEIGEGEEEASRLYPEVNYTR 300

Query: 301 MDDYLKIFL 309
           MDDYLKIFL
Sbjct: 301 MDDYLKIFL 309

BLAST of Csor.00g070150 vs. ExPASy TrEMBL
Match: A0A6J1HLT4 (isoflavone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111464122 PE=4 SV=1)

HSP 1 Score: 587 bits (1513), Expect = 3.21e-211
Identity = 296/309 (95.79%), Postives = 300/309 (97.09%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPAR 120
           SDF SLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNV+RFLPSEYGIDPAR
Sbjct: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVAL 180
           MRDALEPGRETFDQKM +RKAIE+ANIPFTYVSANCFAGYFAGSLSQM +L PPTHQV L
Sbjct: 121 MRDALEPGRETFDQKMIVRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLAPPTHQVTL 180

Query: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240
           YGDGNVK VYVDEDDIATYIVKAATDPRTMNKTVYIRP DNILSQRELIQKWEALS KVL
Sbjct: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPRDNILSQRELIQKWEALSEKVL 240

Query: 241 EKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300
           EKNSVSAQDFL RMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR
Sbjct: 241 EKNSVSAQDFLTRMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300

Query: 301 MDDYLKIFL 309
           MDDYLKIFL
Sbjct: 301 MDDYLKIFL 309

BLAST of Csor.00g070150 vs. ExPASy TrEMBL
Match: A0A6J1HJH3 (isoflavone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111464118 PE=4 SV=1)

HSP 1 Score: 567 bits (1461), Expect = 2.61e-203
Identity = 279/309 (90.29%), Postives = 300/309 (97.09%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGG+GYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGSGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPAR 120
           SDF SLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGN++RFLPSEYGIDPAR
Sbjct: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNIKRFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVAL 180
           M+DALEPGRETFDQKMT+RKAIE+ANIPFTYVSANCFAGYFAGSLS+M +LVPPTHQV L
Sbjct: 121 MKDALEPGRETFDQKMTVRKAIEEANIPFTYVSANCFAGYFAGSLSEMHTLVPPTHQVTL 180

Query: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240
           YGDGNVK VYVDEDDIATY+VKAATDPRTMNKTVYIRPP NILSQRE+++KWEALSGKVL
Sbjct: 181 YGDGNVKAVYVDEDDIATYVVKAATDPRTMNKTVYIRPPHNILSQREMVEKWEALSGKVL 240

Query: 241 EKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300
           EK S+SAQDFLARMKDLD+ Q+AG+GHFYHIFF+GC+TNFE+ E +EEASKLYPEVNYTR
Sbjct: 241 EKKSISAQDFLARMKDLDLGQRAGLGHFYHIFFDGCMTNFEVRE-DEEASKLYPEVNYTR 300

Query: 301 MDDYLKIFL 309
           MDDYLKI+L
Sbjct: 301 MDDYLKIYL 308

BLAST of Csor.00g070150 vs. ExPASy TrEMBL
Match: A0A6J1HWD5 (isoflavone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111466862 PE=4 SV=1)

HSP 1 Score: 565 bits (1456), Expect = 1.51e-202
Identity = 279/309 (90.29%), Postives = 299/309 (96.76%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGG+GYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGSGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPAR 120
           SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGN++RFLPSEYGIDPAR
Sbjct: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNIKRFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVAL 180
           M+DALEPGRETFDQKM +RKAIE+ANIPFTYVSANCFAGYFAGSLS+M +LVPPTHQV L
Sbjct: 121 MKDALEPGRETFDQKMIVRKAIEEANIPFTYVSANCFAGYFAGSLSEMHTLVPPTHQVTL 180

Query: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240
           YGDGNVK VYVDEDDIATY+VKAATDPRTMNKTVYIRPP NILSQRE+++KWEALSGKVL
Sbjct: 181 YGDGNVKAVYVDEDDIATYVVKAATDPRTMNKTVYIRPPHNILSQREMVEKWEALSGKVL 240

Query: 241 EKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300
           EK SVS QDFLARMKDLD+ Q+AG+GHFYHIFF+GC+TNFE+ E +EEASKLYPEVNYTR
Sbjct: 241 EKKSVSPQDFLARMKDLDLGQRAGLGHFYHIFFDGCMTNFEVRE-DEEASKLYPEVNYTR 300

Query: 301 MDDYLKIFL 309
           MDDYLKI+L
Sbjct: 301 MDDYLKIYL 308

BLAST of Csor.00g070150 vs. ExPASy TrEMBL
Match: A0A5A7VJ56 (Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold392G00250 PE=4 SV=1)

HSP 1 Score: 538 bits (1386), Expect = 7.25e-192
Identity = 266/309 (86.08%), Postives = 289/309 (93.53%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           M+VLVVGGTGYLGKRIVKASLLEGH+TYVVQRPEIGLDIEKLQLLLSFKRQGA+LVPASF
Sbjct: 1   MRVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKRQGAILVPASF 60

Query: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPAR 120
           SD QSLVDAVKRVDVVISA+SGVHFRSH++LLQLKLVEAIQ AGN++RFLPSEYGIDP+R
Sbjct: 61  SDIQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIQAAGNIKRFLPSEYGIDPSR 120

Query: 121 MRDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVAL 180
           M +A+E GR+TFDQKM IRKAIE+ANIPFTYVSANCFAGYFA +LSQM++LVPPTHQV +
Sbjct: 121 MGNAIEQGRDTFDQKMVIRKAIEEANIPFTYVSANCFAGYFAANLSQMETLVPPTHQVTV 180

Query: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240
           YGDGNVKV+Y DEDDIA Y+VKAATDPRT+NKTVYIRPP NILSQRELIQ WE LSGKVL
Sbjct: 181 YGDGNVKVIYTDEDDIARYVVKAATDPRTLNKTVYIRPPQNILSQRELIQIWEKLSGKVL 240

Query: 241 EKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300
           EK SVSAQDFLA +KDLD AQQAG GHFY IFF+GCLTNFEIGE  EEASKL+PEVNYT 
Sbjct: 241 EKISVSAQDFLANIKDLDEAQQAGAGHFYEIFFKGCLTNFEIGEAAEEASKLFPEVNYTT 300

Query: 301 MDDYLKIFL 309
           MDDYLKIFL
Sbjct: 301 MDDYLKIFL 309

BLAST of Csor.00g070150 vs. TAIR 10
Match: AT1G32100.1 (pinoresinol reductase 1 )

HSP 1 Score: 443.7 bits (1140), Expect = 1.2e-124
Identity = 214/308 (69.48%), Postives = 259/308 (84.09%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           +VLVVG TGY+GKRIV+A L EGH+TYV+QRPEIGL+IEK+QL LSFK+ GA +V  SFS
Sbjct: 11  RVLVVGATGYIGKRIVRACLAEGHETYVLQRPEIGLEIEKVQLFLSFKKLGARIVEGSFS 70

Query: 62  DFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPARM 121
           D QSLV AVK VDVV+SAMSGVHFRSHN+L+QLKLVEAI+ AGNV+RFLPSE+G+DP RM
Sbjct: 71  DHQSLVSAVKLVDVVVSAMSGVHFRSHNILVQLKLVEAIKEAGNVKRFLPSEFGMDPPRM 130

Query: 122 RDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVALY 181
             AL PGRETFDQKM +R+AIE A IP+TYV   CFA YFAG+LSQM +L+PP  +V +Y
Sbjct: 131 GHALPPGRETFDQKMEVRQAIEAAGIPYTYVVGACFAAYFAGNLSQMVTLLPPKEKVNIY 190

Query: 182 GDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVLE 241
           GDGNVKVV+ DEDDIA Y  K   DPRT+NKTV IRPPDN+L+Q EL+Q WE L+GK LE
Sbjct: 191 GDGNVKVVFADEDDIAKYTAKTLNDPRTLNKTVNIRPPDNVLTQLELVQIWEKLTGKELE 250

Query: 242 KNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTRM 301
           K +++AQDFLA ++ ++I  QAG+GHFYHIF+EGCLT+ E+GE +EEAS LYP+V Y RM
Sbjct: 251 KTNIAAQDFLANIEQMEIPHQAGIGHFYHIFYEGCLTDHEVGE-DEEASSLYPDVKYKRM 310

Query: 302 DDYLKIFL 310
           DDYL++FL
Sbjct: 311 DDYLRMFL 317

BLAST of Csor.00g070150 vs. TAIR 10
Match: AT4G13660.1 (pinoresinol reductase 2 )

HSP 1 Score: 421.8 bits (1083), Expect = 4.8e-118
Identity = 207/308 (67.21%), Postives = 253/308 (82.14%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           +VLVVGGTG LG+RIV A L EGH+TYV+QRPEIG+DIEK+QLLLSFKR GA LV  SFS
Sbjct: 11  RVLVVGGTGSLGRRIVSACLAEGHETYVLQRPEIGVDIEKVQLLLSFKRLGAHLVEGSFS 70

Query: 62  DFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPARM 121
           D QSLV AVK+VDVV+SAMSGVHFR+HN+ +QLKLV AI+ AGNV+RFLPSE+G+DP+RM
Sbjct: 71  DHQSLVSAVKQVDVVVSAMSGVHFRTHNIPVQLKLVAAIKEAGNVKRFLPSEFGMDPSRM 130

Query: 122 RDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVALY 181
             A+ PG ETFDQKM IR AI+ A I  TY+   CFA YF G+LSQM +L PP ++V +Y
Sbjct: 131 GHAMPPGSETFDQKMEIRNAIKAAGISHTYLVGACFAAYFGGNLSQMGTLFPPKNKVDIY 190

Query: 182 GDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVLE 241
           GDGNVKVV+VDEDD+A Y  K   DPRT+NKTVY+RP DNIL+Q EL+Q WE L+ K LE
Sbjct: 191 GDGNVKVVFVDEDDMAKYTAKTLNDPRTLNKTVYVRPTDNILTQMELVQIWEKLTEKELE 250

Query: 242 KNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTRM 301
           K  VS  DFLA ++D +I+ QAG+GHFYHI++EGCLT+ E+G+ +EEA+KLYP+V Y RM
Sbjct: 251 KTYVSGNDFLADIEDKEISHQAGLGHFYHIYYEGCLTDHEVGD-DEEATKLYPDVKYKRM 310

Query: 302 DDYLKIFL 310
           D+YLKIF+
Sbjct: 311 DEYLKIFV 317

BLAST of Csor.00g070150 vs. TAIR 10
Match: AT1G75290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 2.4e-61
Identity = 127/310 (40.97%), Postives = 189/310 (60.97%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           K+LV+GGTG++GK I++AS+  GH T  + R     D  K + + +FK  G  L+    +
Sbjct: 7   KILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHGDLN 66

Query: 62  DFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPARM 121
           D +SLV A+K+ DVVIS +      S  +L Q K++ AI+ AGNV+RFLPSE+G+D  + 
Sbjct: 67  DHESLVKAIKQADVVISTVG-----SMQILDQTKIISAIKEAGNVKRFLPSEFGMDVDK- 126

Query: 122 RDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQS--LVPPTHQVA 181
             A+EP +  F +K+  R+ IE   IP+TY+  N FAGY+  +L Q++     PP  +V 
Sbjct: 127 SSAVEPAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVK 186

Query: 182 LYGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKV 241
           ++GDGNVK V   E+DIA Y +KA  DPRT+NKT+YI PP+N LS  E++  WE   GK 
Sbjct: 187 IFGDGNVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKS 246

Query: 242 LEKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEG-EEEASKLYPEVNY 301
           +EK  +S +     +++  +     +   + +F +G  TNF I      EAS+LYP++ Y
Sbjct: 247 VEKIYMSEEQIFKSIQESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPDIKY 306

Query: 302 TRMDDYLKIF 309
           T +D+YL  F
Sbjct: 307 TSIDEYLSYF 310

BLAST of Csor.00g070150 vs. TAIR 10
Match: AT1G75280.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 229.6 bits (584), Expect = 3.5e-60
Identity = 129/310 (41.61%), Postives = 182/310 (58.71%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           K+LV+GGTGY+GK +V+AS   GH T+ + R     D  K + + SFK  G  ++    +
Sbjct: 7   KILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLN 66

Query: 62  DFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPARM 121
           D +SLV A+K+VDVVIS +      S  +L Q K++ AI+ AGNV+RFLPSE+G+D  R 
Sbjct: 67  DHESLVKAIKQVDVVISTVG-----SMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDR- 126

Query: 122 RDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQS--LVPPTHQVA 181
             A+EP +  F  K+ IR+ IE   IP+TY    CF GY+  +L Q +     PP  +V 
Sbjct: 127 TSAVEPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVT 186

Query: 182 LYGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKV 241
           + GDGN K V   E+DIA Y +KA  DPRT+NK +YI+P +N LS  E++  WE   GK 
Sbjct: 187 ILGDGNAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKS 246

Query: 242 LEKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEG-EEEASKLYPEVNY 301
           LEK  +  +  L  +++  I     +   + +F  G  TN  I      EAS+LYP+V Y
Sbjct: 247 LEKTHLPEEQLLKSIQESPIPINVVLSINHAVFVNGD-TNISIEPSFGVEASELYPDVKY 306

Query: 302 TRMDDYLKIF 309
           T +D+YL  F
Sbjct: 307 TSVDEYLSYF 309

BLAST of Csor.00g070150 vs. TAIR 10
Match: AT1G75300.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 229.2 bits (583), Expect = 4.6e-60
Identity = 133/324 (41.05%), Postives = 189/324 (58.33%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           K+LV+GGTGY+G+ IV+ S   G+ T+ + R     D  K + + SFK  G  ++    +
Sbjct: 7   KILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTILHGDLN 66

Query: 62  DFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPARM 121
           D +SLV A+K+VDVVIS +         +  Q K++ AI+ AGNV+RFLP+E+GID  R 
Sbjct: 67  DHESLVKAIKQVDVVISTIG-----HKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVER- 126

Query: 122 RDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQS----------- 181
             A+EP +  F  K+ IR+AIE   IP+TYV +NC AG++  +L Q +S           
Sbjct: 127 TSAVEPAKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDKAI 186

Query: 182 -----LVPPTHQVALYGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQ 241
                 VPP  +V + GDGN KVV   E+D+A Y++KA  D RT+NKT+YI PP+NILS 
Sbjct: 187 IFGDKNVPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSM 246

Query: 242 RELIQKWEALSGKVLEKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEG 301
            E++  WE   GK LEK  +S +  L   K + +         + +F +G  T+F I   
Sbjct: 247 NEMVTLWEKKIGKSLEKTHISEEQIL---KSIQVPIDVFKSINHAVFVKGDQTSFTIEPW 306

Query: 302 -EEEASKLYPEVNYTRMDDYLKIF 309
             EEAS LYP+V YT +D+YL  F
Sbjct: 307 FGEEASVLYPDVKYTSIDEYLSQF 321

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P525817.7e-13775.32Isoflavone reductase homolog OS=Lupinus albus OX=3870 PE=2 SV=1[more]
A3R0527.2e-12771.84Bifunctional pinoresinol-lariciresinol reductase OS=Linum perenne OX=35941 GN=PL... [more]
Q9FVQ61.7e-12369.48Pinoresinol reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PRR1 PE=1 SV=1[more]
P0DKC81.6e-11867.10Bifunctional pinoresinol-lariciresinol reductase OS=Linum usitatissimum OX=4006 ... [more]
Q4R0H92.8e-11866.77Bifunctional pinoresinol-lariciresinol reductase 1 OS=Linum usitatissimum OX=400... [more]
Match NameE-valueIdentityDescription
KAG6571626.13.34e-220100.00hypothetical protein SDJN03_28354, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022967298.17.53e-21798.06isoflavone reductase homolog [Cucurbita maxima][more]
XP_023511514.11.98e-21296.44isoflavone reductase homolog [Cucurbita pepo subsp. pepo][more]
XP_022963989.16.63e-21195.79isoflavone reductase homolog [Cucurbita moschata][more]
XP_038887505.11.05e-20491.59isoflavone reductase homolog [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1HUP13.65e-21798.06isoflavone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111466861 PE=4 SV... [more]
A0A6J1HLT43.21e-21195.79isoflavone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111464122 PE=4 ... [more]
A0A6J1HJH32.61e-20390.29isoflavone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111464118 PE=4 ... [more]
A0A6J1HWD51.51e-20290.29isoflavone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111466862 PE=4 SV... [more]
A0A5A7VJ567.25e-19286.08Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
AT1G32100.11.2e-12469.48pinoresinol reductase 1 [more]
AT4G13660.14.8e-11867.21pinoresinol reductase 2 [more]
AT1G75290.12.4e-6140.97NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G75280.13.5e-6041.61NmrA-like negative transcriptional regulator family protein [more]
AT1G75300.14.6e-6041.05NmrA-like negative transcriptional regulator family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 2..294
e-value: 7.1E-120
score: 401.6
NoneNo IPR availableGENE3D3.90.25.10coord: 155..305
e-value: 7.1E-120
score: 401.6
NoneNo IPR availablePANTHERPTHR43349PINORESINOL REDUCTASE-RELATEDcoord: 2..306
NoneNo IPR availablePANTHERPTHR43349:SF47BIFUNCTIONAL PINORESINOL-LARICIRESINOL REDUCTASE-LIKEcoord: 2..306
NoneNo IPR availableCDDcd05259PCBER_SDR_acoord: 2..304
e-value: 1.44074E-67
score: 210.622
IPR008030NmrA-like domainPFAMPF05368NmrAcoord: 3..239
e-value: 6.3E-56
score: 189.5
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 2..257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g070150.m01Csor.00g070150.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009807 lignan biosynthetic process
molecular_function GO:0016491 oxidoreductase activity