Csor.00g068280 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g068280
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description2-oxoglutarate and Fe(II)-dependent oxygenase superfamily protein
LocationCsor_Chr15: 5124709 .. 5131619 (+)
RNA-Seq ExpressionCsor.00g068280
SyntenyCsor.00g068280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCTGGAAGCTTCTCTTGAACGACGAAAGCAGCCCCAAGCTCCCGGGACTGGCAATGGAAATGGCGTCGTCTCGCCCAGCTCACACTCTTTCTCAACCCATAGGCTTCGTCTTCAACCCAAGGAAGATCACAAGTCGGAGAGCTATGAGGACCTGCAATTGGAATTCAGCCCGCTCCTCTTCAGCATGCTGGAAAGGCACTTGCCTCCGAGCATGCTCAATATGGCACGCGACCTTAAGCTTCAGTATATGAGGGACATTCTACTCCGATATGCTCCTGAGGGCGAACGCAACCGCGTAAGCGTTTTAAATTGTTTTATCTCTATATCTATGGTGAATTTCAATACATGTTTGCTTGGTTTGGCTGGTGAATGGAGGAGGAGGAAGAGAAGATAGGTTATATTTTCTAGAAATTGTTCTATAGGTTAAAATCAATGATAGCAAATGCCGCATGATCTTATGAATTGTTAGTTGGACGTTGTTTGCAGGCTAAGGATTAAGATTTTTATTTTCATTGTTATGTGATGGTGAATCAGAAGGCGGGAACATAGTTATTTGAACATGTAGATTTGTTAATAGCATGAGGATAGTATTTGTCAAATTTTCTCTTATGCTAGTTAGTCGATGATCTTGATTTCTGACGCAATTTTGATGAAATTAAGGAGGAGGTGTCAATGTTTGTACTTCATTACTCGTTGGGGTTTCTTTCTGTTGGTCTGGGAAGGCAAGGTCTGTTCCTGTATCTTGTTTCTATATTTGATTACTTCCATGTTTCTTGGAAGTATTTCCAGAACAGTGGATTAAATTTTATTTAGACAAAAATGGAGAAAGTATGTGAAGTATGAAGAAAGATAAATATTCCTTCCAAAATCGAAAGCCCCAATAGAGCTTACTAATAGGTGCTCTAGGTAGATGATTAGTAGCAAGGGAATCATTATTGCTAGAAGTTAAGAAGTGAACATCAAGTGGATGCTGCAAAGCAGAGTCAGTCCTCAAACTCAGTCCAAAGTTCTATATAACTCCCATACATTCTCTCATTTCTTTCAAGCTAAAGGTTCCAAATCAGAGTGTTAGAAACAATGATAACAGTTTGACATGCACAGTAACCCAGCATTGCCCGGAGAGAACTTGGTGAGGAATTCATTCAGAGTTCTTTGGAAACACCCCAGCTACTATCTTAAGAACAGAGAATGATTTCTCCGTTATAAAATGCTTGGCTTTCTAAGTTTTATAAACAGTTTATGAACCGATGTTTTTTTAGTTACAACAATTTAATAAAAGCATGAAATGAATGTTAAAATTTACCAAACATATTTTAAAATACATTTTTTTTTTAGAAATGCAAATAATAGGAAATATGGAATGATTTTCAAAAATACTTTAATTTTAAAAAATATTTACTTTTAAAGTATAGAATGGAAAAGAGGAAATATATATAAAGTGTGTGTATTTCTTTCTTCTTTTATTTTATCTTAAAAACAGACCATAAGATAAGAATGATTCACAAAAAACAAATAGTGCTACCCCATTTTGTTATAATTTTTCTTCCAGATAAGTATCATATTCAACTCGGCTTAATTTTATATCATGATAAATAATGTCCCATGTCCATTTTAGCGCTTTCATGGTTTAAATTTCATACCCTTTTAACTCATATCATTTCAACTCTGTTTTCTCAATTTGGAAGATCTTTTTGCTACTTCCAAACTTGGTGCTTGGATTTTCTTTTCTTTTCTTTTTGGTTAATCATACCATCTGCGAATTTTAACATAAAAGAACTCTACTTATTCTATATTTTGGATATAAGAAACACGACTTTCACTGAGATCCAATAACAAGAAACAGGAAAGACTGCAATTAAAAGTACATCATGAGCATGAAAATACCTACAGGGTACTTCTTAGCTTGGACTCTAGGTAAACTCCCATGTCCTAACCAACTATTCTGTCTTAGCTCCTGATACATTTTTATTTGAGTGTGGCATTTTGGTGTGTTGCATGCTGGCAAGCTGATTTCACTTCTGCATGTGCCCATTAAGATTCACAATTTTCAAATACGTTTTCTGTGAACACTCTAACGTGTTTGTTATTTTTCTTGCTTGTAAGAGTCATTCTCTAAGCCTTTTCTTCATTTGTAGCTCCTTTTTCTTCATTTCTTTTGTCAGAAAGCAATAATACATCTATTATTTTGTTTGTTTACTTTTGTAGAAACTTCTTATTAGTTCTTTTAGCTTATTTGGTCGGTTAGAAAGGTGATGTGAAGGAAACTGCCTTTGAATGGGCGTCAATCTGTTGCTTATCTGTTGCATTCCAATCACCTTTTTGTCACCTTCATGATGAATTTTGTGCCTAAGAAACACGATCTCTAGAGTGCATTTAATTGAATATTATGTTTTCATTCATTATCACTTGACAATAACAAAGAGCATCAGAGTAGCTTTCCAAATAAAGACATCTATATGCCTACTATTGTATAGCTTATTGCATTTGATTTAAAAATCCTTGTCTTTGTAGCTGTAAACTTTGATGCCCTTATGGTCATGAGTGCAGACCTTGATAATTCTGCACCTTGAATATAAAATATCCTGGAGTCTTTTCTTCTCTGACTCTAACATTGTAAGGTTAGGTAGTTATATTGTAAGATGATCAATGCGTGTCTAAATTGGTCTGAACACTTTTCAATTATGATTGCTATTATTATTTTTATTAAAAGGAACTCAAATTTCAAATTCACATCATGATGCAAATGTTATTTACCTTGCATTTACTAAAATGATCCAAACGAGGTGCAACAATGAATGAGTGACACGCATCATACATTAAGAAGTTTCCAGATTATATTCATAAATGATGGCGTGATGTGATTGTTGATTATTTATATATTTGTTTCCTTTTGCTTTTCCTTCTGGCTCCTACCATGCGTTGATTGATATTATTGGAATTAGAGAAACATGAAACTTTAAATTTTTAAAAAATAGCCAATGGAAGCTAAATTACTTATTTCATTAACCCATAGAAGTTGTGATGTTTCTATGTATTTTCTTCTTGTGGTGTCCCAAATACTACTTTTCTCATCTGAATTATTCTCTTACCACAATTTCATGCATTTGAATTATCAGATTCAGAGGCATAGAGAATACCGACAAAAGATTATATCAAACTATCAGGTAAATGAAAGCCTTAGCTGTATGTATCTGAGTTAGTCATGCTTAACACTGGTGTAAGAATTTATGGAGTCCATTTTTATATGCTTCAAAATCATCTAATGGCATAATATTTACTTCATAAATTGGAATTTTTGACTTTTGAGTATTTTCAAGGATCAGTTTCCTTGGTTTGTCCTAATGTTACTGGGTTCGAATGGAAATTGCCAAAGAAAAAAGAAAAAAAGAAAAAAGAAACAAAAAAGAAAAAAAAGAAAAAAGAAAACTTGCATTTGTTAATATACTTTTTTAACAATTATCTTTAGTAATAAAATTCATAGAAGATAGATGAGATCTTGATATTTTGTAATGGATCTCCGTTTACATTTTCTTGCTTCAATGAATAGTTACTTACTGTACATCCACAATATATTCCCTTACTAAGTTGCTATCCTACTCTTTGCAGCCATTACACAGGGAGCTTTACAGCATGCATGCTGCGAACTTCTTTGTCCCCTCTTTTCTCAAAGCTATCAATGAGAATTCGGAGGAGAGCTTTCGACGCATCATGTCTGAACCCTCTCCAGGAATTTATAAATTTGAAATGCTTCAACCACAATTCTGTGAAAAGCTGTTATCTGAGGTATAATAATTTAAAATTTGTTTGCATGTTTTTTACTTCCCTGAGATTTGATTTCTGCATAATACTATTTGCCTTTTAATTCTTAGGTAGAAAGCTTTGAAAGATGGGTTCACGAGACAAAATTCAGAATCATGCGACCTAACACAATGAACAAATATGGTGCTGTTCTTGATGACTTTGGTTTGGAGACCATGCTTGATAAGTTGATGGATGATTTTATACGTCCTATATCAAGAGGTAACACTTTTGAGTGAAGTACTCCTTGAAAGTTGTTTCATGTAGAATACGGAGAGCCATGATTTTTCACATGTTTGTTTTTGGTGTTATGTAGTTTTCTTTCCAGAAGTTGGAGGAGCCACACTGGACTCTCATCATGGTTTTGTTGTTGAATATGGAATTGACAGAGATGTGGAACTTGGTAGGTTTTAGTAGGAACTGTTCATTTTCTTGCTTTCCCTCCTATCAATCCTTTTTTATTAATGTTCATAGGGAGAAAAACACATTTTCTTATGAATGGTTTTGTTAAATACAAAATTGTTAGAGATATGGAACATGGTAGTGCTTCCAGTAACTTTTTCTCCACTCGCCAATCTCTTGCTTCTTGCTTAATTTTTTTTTTTTTTTTTTTTTTTAAATTATACCATCTATCCTTGGGCTTTTATTTGTGTCAATTTAATCCTTGTACTTTTTTGAAAGTTTTAATTTCATTACTGTAATTAATTTATCCTAAAAGTTCTTATTGTTAACTAGAGGTGTCAAATTGAATGGAAATTTGATAAAGCATTAATGAAAAGCTATGTGGACTTACTTCACACTGTACGTATGTTATGTGATAATGAGGCGGTCTACACTGTTTTCAACCACAATTGACAACAAATGCCGTTGGAATGAATTATAAGGACAACTATAACTATGACGTTGAAACTTTTTTAAGGACAAAGTTGATACAAACGTGAAACTATAGATAGGAACAATGTTATAATTTAACTTACTTCGTTTTTACTTTTAGTTCTCCACTTTTTCCTTTATGGACATATTTTAGTTATTGAAAATGGAGATCCAGTAAATGAATAGATGATATTAGACTTTAAAAGATCAACCGTAATCTGTTCTTTCTCTTGGCTCATTGCTCAAGACGATTGTTTTTGCTGAAATTTCATTTTTGGTCCTTTTGATATAGTTTCCTATTCATATTTATCTGTAGTTATTGCAATCTGTAAATTTTTGACGTTTTTTGTTTTGTTGCTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATATTTATTTGTTGATATCGAAACTATTAATCAATTTTATTTCAAAATGATTTTACTATAGGTTTTCATGTGGATGATTCGGAAGTCACATTGAATGTTTGTTTGGGTAAACAATTTTCTGGTGGTGAACTGTTCTTTCGCGGCATCCGATGTGACAAGCATGTTAATACGGAGACCCAACCAGAGGTATGATAGTCTTCAGAACACCTTATACCTTTATTTTTGTATTGCTTGTTCCTTCCTTTGTGGTTGGCTTGGCTCATATATTTCAAGCTGCTTATTCAGATCTGACAGTTCTGTGATTGTAAATCTTTAAATTTTCATAAGATTCTTCAAAGATAGATTAGCTTCTTTTGAATCTGCAATCGCATAGATATGAGCTCATTAAAATTAATCATTGATATAAGTCATGAGCTGTTGGGATAATAATACCTTCTTGAAAGCTGCTATTAATATTGAAAAATTCGAACATTTAGGTATATGGGTACATGTAAGCAATAATACTTCATATGTTTACCAGACGATGCAAATGTGTGAATATACATTATACGATCAATGAGTAGTCATTTCATTTTCAGTAATGCTCCTTGGTGGGTGAATTTTATCGTAACAGTCTCTGTATTAGGAAATCTTTGACTATTTGCATGTTCCTGGACATGCGGTTCTTCACCGTGGTCGTCATCGGCATGGTGCTAGAGCTACAACATCTGGGCGTCGGGTCAACTTACTTTTGTGGTGCAGAAGGTATTCTCTCACTAACTTCGACCTCTGAGTGATTGTTTTTTTGTCCAAATAAGAGTAAAAGGATATGATATTTTGTTCAACAACATAGGCACTGATTGGCAGAAAGTGATCATATATATGTATGTATATATGCATGTATGTGTGTGTGTACATATGTATATGAGGTATCACATTGCCCTTTTACATGCCATCCATCCACCGTTTGGGCTGATGCTAAGCACAAAACTTAAGATTAAGAATTAGCTTACATTAGATGAATTGGGTTATGCATATTTCTTTGACCGGAGATGAATTGGTTTACTGTATGCCGAAGTTCTTTTATATTAATATATTATATAGTAAGGCCGGAATCTGAGCACTAAATATATGCTCATGTGATGCTTACTGTTTGAGGTTGCTTGCATTTTTCTGCCAGCTGGAGACAGTTTATGATTGCCCATAAAATTTCTTTTTGACTTGAATTGATCTTTTCTCTTTTTGTTGGCTGGTTTTAAGGTGTGTGGGTGTACTTGTTTTCTTAGTAAAAGCTTGTAGTAAGAACATTTTTACTCGTAATTAATTTAGTAAAAAGTGTTTGATACCAAAATCATTTAAACGTTTGGTTTTATGTATTCTTGATACTGCTTTGGTGCTTCCTAGTCAAGTAGTTCGGCCAAGAGCAGCCAATATCACCTTACAAGTAGAATGCTTTCTCATAAAGCACTTTTAAGTGCTTAAGGTGCTGTTGACATCTTCTAAAAACATAATTTTTGTTACTTTGAAACGGTGTTTTTCAGTAGGAAGCATAGTATTATTATGTACATTATAGATGTATATTGGGCTCATTGCTTTCCGTGTTTCCTAACCCTTTTTTCCTGAAAAATCTTGTTACAGTTCTGTGTTCAGAGAGTTAAAGAAATATCAGAAAGATTTCTCCAGCTGGTGTGGAGAATGCCAACGTGAGAAGAGAGAAAGGCAACTCCTATCAATTGATGCAACAAAGCAGGTGCGTTTCGTTCTAGCTGAATTGAGTACTACTTTTAAGTTTTTACCTCAAATTCAATCTGGTATATCTATTGTCTCCAGGAGTTACTTAGAAGGGACGTAAAATCTCCCCCTTGA

mRNA sequence

ATGTCTCTGGAAGCTTCTCTTGAACGACGAAAGCAGCCCCAAGCTCCCGGGACTGGCAATGGAAATGGCGTCGTCTCGCCCAGCTCACACTCTTTCTCAACCCATAGGCTTCGTCTTCAACCCAAGGAAGATCACAAGTCGGAGAGCTATGAGGACCTGCAATTGGAATTCAGCCCGCTCCTCTTCAGCATGCTGGAAAGGCACTTGCCTCCGAGCATGCTCAATATGGCACGCGACCTTAAGCTTCAGTATATGAGGGACATTCTACTCCGATATGCTCCTGAGGGCGAACGCAACCGCATTCAGAGGCATAGAGAATACCGACAAAAGATTATATCAAACTATCAGCCATTACACAGGGAGCTTTACAGCATGCATGCTGCGAACTTCTTTGTCCCCTCTTTTCTCAAAGCTATCAATGAGAATTCGGAGGAGAGCTTTCGACGCATCATGTCTGAACCCTCTCCAGGAATTTATAAATTTGAAATGCTTCAACCACAATTCTGTGAAAAGCTGTTATCTGAGGTAGAAAGCTTTGAAAGATGGGTTCACGAGACAAAATTCAGAATCATGCGACCTAACACAATGAACAAATATGGTGCTGTTCTTGATGACTTTGGTTTGGAGACCATGCTTGATAAGTTGATGGATGATTTTATACGTCCTATATCAAGAGTTTTCTTTCCAGAAGTTGGAGGAGCCACACTGGACTCTCATCATGGTTTTGTTGTTGAATATGGAATTGACAGAGATGTGGAACTTGGTTTTCATGTGGATGATTCGGAAGTCACATTGAATGTTTGTTTGGGTAAACAATTTTCTGGTGGTGAACTGTTCTTTCGCGGCATCCGATGTGACAAGCATGTTAATACGGAGACCCAACCAGAGGAAATCTTTGACTATTTGCATGTTCCTGGACATGCGGTTCTTCACCGTGGTCGTCATCGGCATGGTGCTAGAGCTACAACATCTGGGCGTCGGGTCAACTTACTTTTGTGGTGCAGAAGTTCTGTGTTCAGAGAGTTAAAGAAATATCAGAAAGATTTCTCCAGCTGGTGTGGAGAATGCCAACGTGAGAAGAGAGAAAGGCAACTCCTATCAATTGATGCAACAAAGCAGGAGTTACTTAGAAGGGACGTAAAATCTCCCCCTTGA

Coding sequence (CDS)

ATGTCTCTGGAAGCTTCTCTTGAACGACGAAAGCAGCCCCAAGCTCCCGGGACTGGCAATGGAAATGGCGTCGTCTCGCCCAGCTCACACTCTTTCTCAACCCATAGGCTTCGTCTTCAACCCAAGGAAGATCACAAGTCGGAGAGCTATGAGGACCTGCAATTGGAATTCAGCCCGCTCCTCTTCAGCATGCTGGAAAGGCACTTGCCTCCGAGCATGCTCAATATGGCACGCGACCTTAAGCTTCAGTATATGAGGGACATTCTACTCCGATATGCTCCTGAGGGCGAACGCAACCGCATTCAGAGGCATAGAGAATACCGACAAAAGATTATATCAAACTATCAGCCATTACACAGGGAGCTTTACAGCATGCATGCTGCGAACTTCTTTGTCCCCTCTTTTCTCAAAGCTATCAATGAGAATTCGGAGGAGAGCTTTCGACGCATCATGTCTGAACCCTCTCCAGGAATTTATAAATTTGAAATGCTTCAACCACAATTCTGTGAAAAGCTGTTATCTGAGGTAGAAAGCTTTGAAAGATGGGTTCACGAGACAAAATTCAGAATCATGCGACCTAACACAATGAACAAATATGGTGCTGTTCTTGATGACTTTGGTTTGGAGACCATGCTTGATAAGTTGATGGATGATTTTATACGTCCTATATCAAGAGTTTTCTTTCCAGAAGTTGGAGGAGCCACACTGGACTCTCATCATGGTTTTGTTGTTGAATATGGAATTGACAGAGATGTGGAACTTGGTTTTCATGTGGATGATTCGGAAGTCACATTGAATGTTTGTTTGGGTAAACAATTTTCTGGTGGTGAACTGTTCTTTCGCGGCATCCGATGTGACAAGCATGTTAATACGGAGACCCAACCAGAGGAAATCTTTGACTATTTGCATGTTCCTGGACATGCGGTTCTTCACCGTGGTCGTCATCGGCATGGTGCTAGAGCTACAACATCTGGGCGTCGGGTCAACTTACTTTTGTGGTGCAGAAGTTCTGTGTTCAGAGAGTTAAAGAAATATCAGAAAGATTTCTCCAGCTGGTGTGGAGAATGCCAACGTGAGAAGAGAGAAAGGCAACTCCTATCAATTGATGCAACAAAGCAGGAGTTACTTAGAAGGGACGTAAAATCTCCCCCTTGA

Protein sequence

MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPLLFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHRELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFERWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHHGFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFDYLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREKRERQLLSIDATKQELLRRDVKSPP
Homology
BLAST of Csor.00g068280 vs. ExPASy Swiss-Prot
Match: P0DO23 (2-oxoglutarate and iron-dependent oxygenase domain-containing protein CP2 OS=Arabidopsis thaliana OX=3702 GN=CP2 PE=1 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 1.7e-146
Identity = 254/387 (65.63%), Postives = 309/387 (79.84%), Query Frame = 0

Query: 6   SLERRK--QPQAPGTGNGNGVVS-PSSHS------------FSTHRLRLQPKEDHKSESY 65
           S E+R+  Q     T  GNG ++  +SHS             S  RLRL P  +H+ +SY
Sbjct: 2   SSEQREGSQETTTTTVEGNGTIAGQNSHSAAPTTLRATSTMVSCQRLRLNPNNEHRPDSY 61

Query: 66  EDLQLEFSPLLFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQK 125
           EDLQL+F   ++S LE++LPP+ML   RD K+++M DI+LR+ P GER+R QRH +YR K
Sbjct: 62  EDLQLDFPNSVYSSLEKYLPPNMLVSNRDEKIKFMTDIMLRHLPHGERSRAQRHSDYRLK 121

Query: 126 IISNYQPLHRELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCE 185
           I +NYQPLH+ELY++     FVP+FLKAINEN+EESFR I+SEPSPG++ F+MLQP FCE
Sbjct: 122 ITTNYQPLHKELYTLVPTVCFVPAFLKAINENTEESFRNIISEPSPGVFVFDMLQPSFCE 181

Query: 186 KLLSEVESFERWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPE 245
            +L+E+++FERWV ETKFRIMRPNTMNKYGAVLDDFGL+TMLDKLM+ FIRPIS+VFF +
Sbjct: 182 MMLAEIDNFERWVGETKFRIMRPNTMNKYGAVLDDFGLDTMLDKLMEGFIRPISKVFFSD 241

Query: 246 VGGATLDSHHGFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVN 305
           VGGATLDSHHGFVVEYG DRDV+LGFHVDDSEVTLNVCLG QF GGELFFRG RC+KHVN
Sbjct: 242 VGGATLDSHHGFVVEYGKDRDVDLGFHVDDSEVTLNVCLGNQFVGGELFFRGTRCEKHVN 301

Query: 306 TETQPEEIFDYLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFS 365
           T T+ +E +DY H+PG AVLHRGRHRHGARATT G RVN+LLWCRSSVFRELK + KDFS
Sbjct: 302 TATKADETYDYCHIPGQAVLHRGRHRHGARATTCGHRVNMLLWCRSSVFRELKTHHKDFS 361

Query: 366 SWCGECQREKRERQLLSIDATKQELLR 378
           SWCGEC  EKR+ ++ SIDA +++L +
Sbjct: 362 SWCGECFCEKRDEKVRSIDALRKKLFK 388

BLAST of Csor.00g068280 vs. ExPASy Swiss-Prot
Match: Q3ED68 (2-oxoglutarate and iron-dependent oxygenase domain-containing protein ICU11 OS=Arabidopsis thaliana OX=3702 GN=ICU11 PE=2 SV=2)

HSP 1 Score: 477.6 bits (1228), Expect = 1.3e-133
Identity = 226/380 (59.47%), Postives = 296/380 (77.89%), Query Frame = 0

Query: 1   MSLEASLER--RKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFS 60
           M+L++S ++  ++Q Q P   +GNG         +  +LR  P E+H+ E+YEDL L++S
Sbjct: 10  MALDSSGKQPEQQQQQQPRASSGNGE--------ARLKLRRTPNEEHEPENYEDLPLDYS 69

Query: 61  PLLFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPL 120
           P LF+ LER+LP  +LN  R  K  +MRD+LLRY+P+ ER R+ RH+EYR KI+S+YQ L
Sbjct: 70  PSLFTSLERYLPEQLLNSTRIDKASFMRDLLLRYSPDTERVRVLRHKEYRDKIMSSYQRL 129

Query: 121 HRELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVES 180
           H E+Y++  ++FF PSFL A +  SE +FR  M E  PGI+ FEM +PQFCE LL+EVE 
Sbjct: 130 HGEIYTLDPSSFFAPSFLGAFSRKSEPNFRSSMVESYPGIFTFEMFKPQFCEMLLAEVEH 189

Query: 181 FERWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDS 240
            E+WV++++  IMRPNTMN +G VLDDFG ++ML KL+DDFI PI++V FPEV G +LDS
Sbjct: 190 MEKWVYDSRSTIMRPNTMNNFGVVLDDFGFDSMLQKLVDDFISPIAQVLFPEVCGTSLDS 249

Query: 241 HHGFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEI 300
           HHG++VEYG DRDV+LGFHVDDSEV+LNVCLGKQFSGGEL+FRG+RCDKHVN+++  +E+
Sbjct: 250 HHGYIVEYGKDRDVDLGFHVDDSEVSLNVCLGKQFSGGELYFRGVRCDKHVNSDSTEKEV 309

Query: 301 FDYLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQR 360
           +DY HVPGHA+LHRGRHRHGARATTSG R NL+LWCRSS FRE+K YQ+DFS WCG C+ 
Sbjct: 310 YDYSHVPGHAILHRGRHRHGARATTSGHRANLILWCRSSTFREMKNYQRDFSGWCGGCKL 369

Query: 361 EKRERQLLSIDATKQELLRR 379
           +K+ RQ  SI+ATK+ L R+
Sbjct: 370 DKQRRQRDSINATKEILARK 381

BLAST of Csor.00g068280 vs. ExPASy Swiss-Prot
Match: Q28C22 (2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2 OS=Xenopus tropicalis OX=8364 GN=ogfod2 PE=2 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 8.3e-40
Identity = 98/267 (36.70%), Postives = 149/267 (55.81%), Query Frame = 0

Query: 83  QYMRDILLRYAPEGERNRI--QRHREYRQKIISNYQPLHRELYSMHAANFFVPSFLKAI- 142
           +  R++L     E ER R   +     R++I  +Y+PL+ E+Y +   +F    FL A+ 
Sbjct: 53  EQFRNVLETIKKEVERRRKLGEESLHRRREISLHYKPLYPEVYVLQ-ESFLAAEFLTAVK 112

Query: 143 -----NENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFERWVHETKFRIMRPN 202
                  N E     + S     IY+  +  P+FC KL+ E+E+FER    +     RPN
Sbjct: 113 YSKSPQANVEGLLHHLHSITDKRIYRLPVFIPEFCAKLVEELENFER----SDLPKGRPN 172

Query: 203 TMNKYGAVLDDFG-LETMLDKLMDDFIRPISRVFFPEVGGATLDSHHGFVVEYGIDRDVE 262
           TMN YG +L++ G ++ +   L + +I P++ + FP+ GG  LDSH  FVV+Y +  D++
Sbjct: 173 TMNNYGILLNELGFVDALTAPLCEKYIEPLTSLLFPDWGGGCLDSHRAFVVKYALQEDLD 232

Query: 263 LGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFDYLHVPGHAVLHRG 322
           L  H D++EVTLNV LGK+F+ G L+F  ++ +  VN  T  E      H+ G  +LHRG
Sbjct: 233 LSCHYDNAEVTLNVSLGKEFTDGNLYFSDMK-EVPVNERTYAE----VEHITGQGILHRG 292

Query: 323 RHRHGARATTSGRRVNLLLWCRSSVFR 341
           +H HGA   +SG R NL+LW R+S  R
Sbjct: 293 QHVHGALPISSGERWNLILWMRASDVR 309

BLAST of Csor.00g068280 vs. ExPASy Swiss-Prot
Match: A3KGZ2 (2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2 OS=Danio rerio OX=7955 GN=ogfod2 PE=2 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 8.6e-37
Identity = 91/261 (34.87%), Postives = 142/261 (54.41%), Query Frame = 0

Query: 86  RDILLRYAPEGER--NRIQRHREYRQKIISNYQPLHRELYSMHAANFFVPSFLKAI---- 145
           RD++ +   E ER  N   +  E    I   Y PLH+ +Y +   +F  P  L+ +    
Sbjct: 52  RDVIGKIQAEIERRQNHKLKSTERAAVIKEIYTPLHQHVYHLQ-ESFLAPELLEMVKYCA 111

Query: 146 --NENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFERWVHETKFRIMRPNTMN 205
               N +   + I +E +  +++F++ + +FC+ LL E+E FE    ++     RPNTMN
Sbjct: 112 SSEANVQGLLKLIQTEAASRVFRFQVFRKEFCKDLLEELEHFE----QSDAPKGRPNTMN 171

Query: 206 KYGAVLDDFGL-ETMLDKLMDDFIRPISRVFFPEVGGATLDSHHGFVVEYGIDRDVELGF 265
            YG VL++ G  E  +  L + ++RP++ + + + GG  LDSH  FVV+Y +  D+ L +
Sbjct: 172 NYGIVLNELGFDEGFITPLREVYLRPLTALLYSDCGGNCLDSHKAFVVKYDMHEDLNLSY 231

Query: 266 HVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFDYLHVPGHAVLHRGRHR 325
           H D+SEVTLNV LGK F+ G LFF  +R            E  +  H     +LHRG+H 
Sbjct: 232 HYDNSEVTLNVSLGKDFTEGNLFFGDMR-----QVPLSETECVEVEHRVTEGLLHRGQHM 291

Query: 326 HGARATTSGRRVNLLLWCRSS 338
           HGA + +SG R NL++W R+S
Sbjct: 292 HGALSISSGTRWNLIIWMRAS 302

BLAST of Csor.00g068280 vs. ExPASy Swiss-Prot
Match: Q6N063 (2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2 OS=Homo sapiens OX=9606 GN=OGFOD2 PE=1 SV=2)

HSP 1 Score: 153.3 bits (386), Expect = 5.6e-36
Identity = 98/272 (36.03%), Postives = 141/272 (51.84%), Query Frame = 0

Query: 98  RNRIQRHREYRQKII-SNYQPLHRELYSMHAANFFVPSFLKAINENS---EESFRRIMS- 157
           R R+ +    R+ +I S+Y P   E+Y         P FL A+ E S   +   + ++  
Sbjct: 71  RQRLGQESAARKALIASSYHPARPEVYDSLQDAALAPEFL-AVTEYSVSPDADLKGLLQR 130

Query: 158 ----EPSPGIYKFEMLQPQFCEKLLSEVESFERWVHETKFRIMRPNTMNKYGAVLDDFGL 217
                    IY+  +    FC+ LL E+E FE    ++     RPNTMN YG +L + GL
Sbjct: 131 LETVSEEKRIYRVPVFTAPFCQALLEELEHFE----QSDMPKGRPNTMNNYGVLLHELGL 190

Query: 218 -ETMLDKLMDDFIRPISRVFFPEVGGATLDSHHGFVVEYGIDRDVELGFHVDDSEVTLNV 277
            E ++  L + F++P+  + +P+ GG  LDSH  FVV+Y   +D+ELG H D++E+TLNV
Sbjct: 191 DEPLMTPLRERFLQPLMALLYPDCGGGRLDSHRAFVVKYAPGQDLELGCHYDNAELTLNV 250

Query: 278 CLGKQFSGGELFFRGIRCDKHVNTETQPEEIFDYLHVPGHAVLHRGRHRHGARATTSGRR 337
            LGK F+GG L+F G+         T   E  +  HV G  VLHRG   HGAR   +G R
Sbjct: 251 ALGKVFTGGALYFGGL-----FQAPTALTEPLEVEHVVGQGVLHRGGQLHGARPLGTGER 310

Query: 338 VNLLLWCRSSVFRELKKYQKDFSSWCGECQRE 360
            NL++W R+S  R         +S C  C RE
Sbjct: 311 WNLVVWLRASAVR---------NSLCPMCCRE 323

BLAST of Csor.00g068280 vs. NCBI nr
Match: XP_022938873.1 (uncharacterized PKHD-type hydroxylase At1g22950-like [Cucurbita moschata] >XP_022938874.1 uncharacterized PKHD-type hydroxylase At1g22950-like [Cucurbita moschata] >XP_022938875.1 uncharacterized PKHD-type hydroxylase At1g22950-like [Cucurbita moschata] >XP_022993289.1 uncharacterized PKHD-type hydroxylase At1g22950-like [Cucurbita maxima] >XP_022993291.1 uncharacterized PKHD-type hydroxylase At1g22950-like [Cucurbita maxima] >XP_022993292.1 uncharacterized PKHD-type hydroxylase At1g22950-like [Cucurbita maxima] >KAG6579227.1 2-oxoglutarate and iron-dependent oxygenase domain-containing protein CP2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016740.1 putative PKHD-type hydroxylase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 783 bits (2022), Expect = 5.91e-286
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0

Query: 1   MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL 60
           MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL
Sbjct: 1   MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL 60

Query: 61  LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR 120
           LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR
Sbjct: 61  LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR 120

Query: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180
           ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE
Sbjct: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180

Query: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240
           RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH
Sbjct: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240

Query: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD 300
           GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD
Sbjct: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD 300

Query: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360
           YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK
Sbjct: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360

Query: 361 RERQLLSIDATKQELLRRDVKSPP 384
           RERQLLSIDATKQELLRRDVKSPP
Sbjct: 361 RERQLLSIDATKQELLRRDVKSPP 384

BLAST of Csor.00g068280 vs. NCBI nr
Match: XP_023550033.1 (uncharacterized PKHD-type hydroxylase At1g22950-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 782 bits (2019), Expect = 1.69e-285
Identity = 383/384 (99.74%), Postives = 384/384 (100.00%), Query Frame = 0

Query: 1   MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL 60
           MSLEASLERRKQPQAPGTGNGNGVVSPS+HSFSTHRLRLQPKEDHKSESYEDLQLEFSPL
Sbjct: 1   MSLEASLERRKQPQAPGTGNGNGVVSPSAHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL 60

Query: 61  LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR 120
           LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR
Sbjct: 61  LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR 120

Query: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180
           ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE
Sbjct: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180

Query: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240
           RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH
Sbjct: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240

Query: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD 300
           GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD
Sbjct: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD 300

Query: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360
           YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK
Sbjct: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360

Query: 361 RERQLLSIDATKQELLRRDVKSPP 384
           RERQLLSIDATKQELLRRDVKSPP
Sbjct: 361 RERQLLSIDATKQELLRRDVKSPP 384

BLAST of Csor.00g068280 vs. NCBI nr
Match: XP_038875561.1 (2-oxoglutarate and iron-dependent oxygenase domain-containing protein CP2-like [Benincasa hispida])

HSP 1 Score: 767 bits (1981), Expect = 1.05e-279
Identity = 375/384 (97.66%), Postives = 380/384 (98.96%), Query Frame = 0

Query: 1   MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL 60
           MSLEASLERRKQPQAPGTGNGNGVV P + SFSTHRLRLQPKEDHKSESYEDLQLEFSP+
Sbjct: 1   MSLEASLERRKQPQAPGTGNGNGVVPPPAQSFSTHRLRLQPKEDHKSESYEDLQLEFSPV 60

Query: 61  LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR 120
           LFSMLERHLPPSMLN+ARDLKLQYMRDILLRYAPEGERNR+QRHREYRQKIISNYQPLHR
Sbjct: 61  LFSMLERHLPPSMLNVARDLKLQYMRDILLRYAPEGERNRVQRHREYRQKIISNYQPLHR 120

Query: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180
           ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE
Sbjct: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180

Query: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240
           RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH
Sbjct: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240

Query: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD 300
           GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQ EEIFD
Sbjct: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQSEEIFD 300

Query: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360
           YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK
Sbjct: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360

Query: 361 RERQLLSIDATKQELLRRDVKSPP 384
           RERQLLSIDATKQELLRR+VKSPP
Sbjct: 361 RERQLLSIDATKQELLRREVKSPP 384

BLAST of Csor.00g068280 vs. NCBI nr
Match: XP_004147120.1 (uncharacterized PKHD-type hydroxylase At1g22950 [Cucumis sativus] >KGN51525.1 hypothetical protein Csa_009158 [Cucumis sativus])

HSP 1 Score: 763 bits (1970), Expect = 4.99e-278
Identity = 372/384 (96.88%), Postives = 380/384 (98.96%), Query Frame = 0

Query: 1   MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL 60
           MSLEASLERRKQPQAPGTGNGNGVVSP+  S STHRLRLQPKEDHKSESYEDLQLEFSP+
Sbjct: 1   MSLEASLERRKQPQAPGTGNGNGVVSPTPQSLSTHRLRLQPKEDHKSESYEDLQLEFSPV 60

Query: 61  LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR 120
           LFSMLERHLPP+MLN+AR++KLQYMRDILLRYAPEGERNR+QRHREYRQKIISNYQPLHR
Sbjct: 61  LFSMLERHLPPNMLNVAREVKLQYMRDILLRYAPEGERNRVQRHREYRQKIISNYQPLHR 120

Query: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180
           ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE
Sbjct: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180

Query: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240
           RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH
Sbjct: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240

Query: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD 300
           GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQ EEIFD
Sbjct: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQSEEIFD 300

Query: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360
           YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK
Sbjct: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360

Query: 361 RERQLLSIDATKQELLRRDVKSPP 384
           RERQLLSIDATKQELLRR+VKSPP
Sbjct: 361 RERQLLSIDATKQELLRREVKSPP 384

BLAST of Csor.00g068280 vs. NCBI nr
Match: XP_008467170.1 (PREDICTED: uncharacterized PKHD-type hydroxylase At1g22950-like [Cucumis melo] >KAA0046297.1 putative PKHD-type hydroxylase [Cucumis melo var. makuwa] >TYK30462.1 putative PKHD-type hydroxylase [Cucumis melo var. makuwa])

HSP 1 Score: 763 bits (1969), Expect = 7.09e-278
Identity = 371/384 (96.61%), Postives = 380/384 (98.96%), Query Frame = 0

Query: 1   MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL 60
           MSLEASLERRKQPQAPGTGNGNGVVSP+  S STHRLRLQPKEDHKSESYEDLQLEFSP+
Sbjct: 1   MSLEASLERRKQPQAPGTGNGNGVVSPTPQSLSTHRLRLQPKEDHKSESYEDLQLEFSPV 60

Query: 61  LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR 120
           LFSMLERHLPP+MLN+AR++KLQYMRDILLRYAPEGERNR+QRHREYRQKIISNYQPLHR
Sbjct: 61  LFSMLERHLPPNMLNVAREVKLQYMRDILLRYAPEGERNRVQRHREYRQKIISNYQPLHR 120

Query: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180
           ELYSMHAANFFVPSFLKA+NENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE
Sbjct: 121 ELYSMHAANFFVPSFLKAVNENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180

Query: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240
           RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH
Sbjct: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240

Query: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD 300
           GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQ EEIFD
Sbjct: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQSEEIFD 300

Query: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360
           YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK
Sbjct: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360

Query: 361 RERQLLSIDATKQELLRRDVKSPP 384
           RERQLLSIDATKQELLRR+VKSPP
Sbjct: 361 RERQLLSIDATKQELLRREVKSPP 384

BLAST of Csor.00g068280 vs. ExPASy TrEMBL
Match: A0A6J1JSE2 (uncharacterized PKHD-type hydroxylase At1g22950-like OS=Cucurbita maxima OX=3661 GN=LOC111489347 PE=4 SV=1)

HSP 1 Score: 783 bits (2022), Expect = 2.86e-286
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0

Query: 1   MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL 60
           MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL
Sbjct: 1   MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL 60

Query: 61  LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR 120
           LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR
Sbjct: 61  LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR 120

Query: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180
           ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE
Sbjct: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180

Query: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240
           RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH
Sbjct: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240

Query: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD 300
           GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD
Sbjct: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD 300

Query: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360
           YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK
Sbjct: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360

Query: 361 RERQLLSIDATKQELLRRDVKSPP 384
           RERQLLSIDATKQELLRRDVKSPP
Sbjct: 361 RERQLLSIDATKQELLRRDVKSPP 384

BLAST of Csor.00g068280 vs. ExPASy TrEMBL
Match: A0A6J1FFE1 (uncharacterized PKHD-type hydroxylase At1g22950-like OS=Cucurbita moschata OX=3662 GN=LOC111444950 PE=4 SV=1)

HSP 1 Score: 783 bits (2022), Expect = 2.86e-286
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0

Query: 1   MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL 60
           MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL
Sbjct: 1   MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL 60

Query: 61  LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR 120
           LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR
Sbjct: 61  LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR 120

Query: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180
           ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE
Sbjct: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180

Query: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240
           RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH
Sbjct: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240

Query: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD 300
           GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD
Sbjct: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD 300

Query: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360
           YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK
Sbjct: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360

Query: 361 RERQLLSIDATKQELLRRDVKSPP 384
           RERQLLSIDATKQELLRRDVKSPP
Sbjct: 361 RERQLLSIDATKQELLRRDVKSPP 384

BLAST of Csor.00g068280 vs. ExPASy TrEMBL
Match: A0A0A0KPJ6 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G576670 PE=4 SV=1)

HSP 1 Score: 763 bits (1970), Expect = 2.42e-278
Identity = 372/384 (96.88%), Postives = 380/384 (98.96%), Query Frame = 0

Query: 1   MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL 60
           MSLEASLERRKQPQAPGTGNGNGVVSP+  S STHRLRLQPKEDHKSESYEDLQLEFSP+
Sbjct: 1   MSLEASLERRKQPQAPGTGNGNGVVSPTPQSLSTHRLRLQPKEDHKSESYEDLQLEFSPV 60

Query: 61  LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR 120
           LFSMLERHLPP+MLN+AR++KLQYMRDILLRYAPEGERNR+QRHREYRQKIISNYQPLHR
Sbjct: 61  LFSMLERHLPPNMLNVAREVKLQYMRDILLRYAPEGERNRVQRHREYRQKIISNYQPLHR 120

Query: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180
           ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE
Sbjct: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180

Query: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240
           RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH
Sbjct: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240

Query: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD 300
           GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQ EEIFD
Sbjct: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQSEEIFD 300

Query: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360
           YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK
Sbjct: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360

Query: 361 RERQLLSIDATKQELLRRDVKSPP 384
           RERQLLSIDATKQELLRR+VKSPP
Sbjct: 361 RERQLLSIDATKQELLRREVKSPP 384

BLAST of Csor.00g068280 vs. ExPASy TrEMBL
Match: A0A5A7TWL9 (Putative PKHD-type hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00020 PE=4 SV=1)

HSP 1 Score: 763 bits (1969), Expect = 3.43e-278
Identity = 371/384 (96.61%), Postives = 380/384 (98.96%), Query Frame = 0

Query: 1   MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL 60
           MSLEASLERRKQPQAPGTGNGNGVVSP+  S STHRLRLQPKEDHKSESYEDLQLEFSP+
Sbjct: 1   MSLEASLERRKQPQAPGTGNGNGVVSPTPQSLSTHRLRLQPKEDHKSESYEDLQLEFSPV 60

Query: 61  LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR 120
           LFSMLERHLPP+MLN+AR++KLQYMRDILLRYAPEGERNR+QRHREYRQKIISNYQPLHR
Sbjct: 61  LFSMLERHLPPNMLNVAREVKLQYMRDILLRYAPEGERNRVQRHREYRQKIISNYQPLHR 120

Query: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180
           ELYSMHAANFFVPSFLKA+NENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE
Sbjct: 121 ELYSMHAANFFVPSFLKAVNENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180

Query: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240
           RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH
Sbjct: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240

Query: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD 300
           GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQ EEIFD
Sbjct: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQSEEIFD 300

Query: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360
           YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK
Sbjct: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360

Query: 361 RERQLLSIDATKQELLRRDVKSPP 384
           RERQLLSIDATKQELLRR+VKSPP
Sbjct: 361 RERQLLSIDATKQELLRREVKSPP 384

BLAST of Csor.00g068280 vs. ExPASy TrEMBL
Match: A0A1S3CSW6 (uncharacterized PKHD-type hydroxylase At1g22950-like OS=Cucumis melo OX=3656 GN=LOC103504586 PE=4 SV=1)

HSP 1 Score: 763 bits (1969), Expect = 3.43e-278
Identity = 371/384 (96.61%), Postives = 380/384 (98.96%), Query Frame = 0

Query: 1   MSLEASLERRKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFSPL 60
           MSLEASLERRKQPQAPGTGNGNGVVSP+  S STHRLRLQPKEDHKSESYEDLQLEFSP+
Sbjct: 1   MSLEASLERRKQPQAPGTGNGNGVVSPTPQSLSTHRLRLQPKEDHKSESYEDLQLEFSPV 60

Query: 61  LFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPLHR 120
           LFSMLERHLPP+MLN+AR++KLQYMRDILLRYAPEGERNR+QRHREYRQKIISNYQPLHR
Sbjct: 61  LFSMLERHLPPNMLNVAREVKLQYMRDILLRYAPEGERNRVQRHREYRQKIISNYQPLHR 120

Query: 121 ELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180
           ELYSMHAANFFVPSFLKA+NENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE
Sbjct: 121 ELYSMHAANFFVPSFLKAVNENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVESFE 180

Query: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240
           RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH
Sbjct: 181 RWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDSHH 240

Query: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFD 300
           GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQ EEIFD
Sbjct: 241 GFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQSEEIFD 300

Query: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360
           YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK
Sbjct: 301 YLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREK 360

Query: 361 RERQLLSIDATKQELLRRDVKSPP 384
           RERQLLSIDATKQELLRR+VKSPP
Sbjct: 361 RERQLLSIDATKQELLRREVKSPP 384

BLAST of Csor.00g068280 vs. TAIR 10
Match: AT3G18210.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 520.4 bits (1339), Expect = 1.2e-147
Identity = 254/387 (65.63%), Postives = 309/387 (79.84%), Query Frame = 0

Query: 6   SLERRK--QPQAPGTGNGNGVVS-PSSHS------------FSTHRLRLQPKEDHKSESY 65
           S E+R+  Q     T  GNG ++  +SHS             S  RLRL P  +H+ +SY
Sbjct: 2   SSEQREGSQETTTTTVEGNGTIAGQNSHSAAPTTLRATSTMVSCQRLRLNPNNEHRPDSY 61

Query: 66  EDLQLEFSPLLFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQK 125
           EDLQL+F   ++S LE++LPP+ML   RD K+++M DI+LR+ P GER+R QRH +YR K
Sbjct: 62  EDLQLDFPNSVYSSLEKYLPPNMLVSNRDEKIKFMTDIMLRHLPHGERSRAQRHSDYRLK 121

Query: 126 IISNYQPLHRELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCE 185
           I +NYQPLH+ELY++     FVP+FLKAINEN+EESFR I+SEPSPG++ F+MLQP FCE
Sbjct: 122 ITTNYQPLHKELYTLVPTVCFVPAFLKAINENTEESFRNIISEPSPGVFVFDMLQPSFCE 181

Query: 186 KLLSEVESFERWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPE 245
            +L+E+++FERWV ETKFRIMRPNTMNKYGAVLDDFGL+TMLDKLM+ FIRPIS+VFF +
Sbjct: 182 MMLAEIDNFERWVGETKFRIMRPNTMNKYGAVLDDFGLDTMLDKLMEGFIRPISKVFFSD 241

Query: 246 VGGATLDSHHGFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVN 305
           VGGATLDSHHGFVVEYG DRDV+LGFHVDDSEVTLNVCLG QF GGELFFRG RC+KHVN
Sbjct: 242 VGGATLDSHHGFVVEYGKDRDVDLGFHVDDSEVTLNVCLGNQFVGGELFFRGTRCEKHVN 301

Query: 306 TETQPEEIFDYLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFS 365
           T T+ +E +DY H+PG AVLHRGRHRHGARATT G RVN+LLWCRSSVFRELK + KDFS
Sbjct: 302 TATKADETYDYCHIPGQAVLHRGRHRHGARATTCGHRVNMLLWCRSSVFRELKTHHKDFS 361

Query: 366 SWCGECQREKRERQLLSIDATKQELLR 378
           SWCGEC  EKR+ ++ SIDA +++L +
Sbjct: 362 SWCGECFCEKRDEKVRSIDALRKKLFK 388

BLAST of Csor.00g068280 vs. TAIR 10
Match: AT3G18210.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 520.4 bits (1339), Expect = 1.2e-147
Identity = 254/387 (65.63%), Postives = 309/387 (79.84%), Query Frame = 0

Query: 6   SLERRK--QPQAPGTGNGNGVVS-PSSHS------------FSTHRLRLQPKEDHKSESY 65
           S E+R+  Q     T  GNG ++  +SHS             S  RLRL P  +H+ +SY
Sbjct: 2   SSEQREGSQETTTTTVEGNGTIAGQNSHSAAPTTLRATSTMVSCQRLRLNPNNEHRPDSY 61

Query: 66  EDLQLEFSPLLFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQK 125
           EDLQL+F   ++S LE++LPP+ML   RD K+++M DI+LR+ P GER+R QRH +YR K
Sbjct: 62  EDLQLDFPNSVYSSLEKYLPPNMLVSNRDEKIKFMTDIMLRHLPHGERSRAQRHSDYRLK 121

Query: 126 IISNYQPLHRELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCE 185
           I +NYQPLH+ELY++     FVP+FLKAINEN+EESFR I+SEPSPG++ F+MLQP FCE
Sbjct: 122 ITTNYQPLHKELYTLVPTVCFVPAFLKAINENTEESFRNIISEPSPGVFVFDMLQPSFCE 181

Query: 186 KLLSEVESFERWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPE 245
            +L+E+++FERWV ETKFRIMRPNTMNKYGAVLDDFGL+TMLDKLM+ FIRPIS+VFF +
Sbjct: 182 MMLAEIDNFERWVGETKFRIMRPNTMNKYGAVLDDFGLDTMLDKLMEGFIRPISKVFFSD 241

Query: 246 VGGATLDSHHGFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVN 305
           VGGATLDSHHGFVVEYG DRDV+LGFHVDDSEVTLNVCLG QF GGELFFRG RC+KHVN
Sbjct: 242 VGGATLDSHHGFVVEYGKDRDVDLGFHVDDSEVTLNVCLGNQFVGGELFFRGTRCEKHVN 301

Query: 306 TETQPEEIFDYLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFS 365
           T T+ +E +DY H+PG AVLHRGRHRHGARATT G RVN+LLWCRSSVFRELK + KDFS
Sbjct: 302 TATKADETYDYCHIPGQAVLHRGRHRHGARATTCGHRVNMLLWCRSSVFRELKTHHKDFS 361

Query: 366 SWCGECQREKRERQLLSIDATKQELLR 378
           SWCGEC  EKR+ ++ SIDA +++L +
Sbjct: 362 SWCGECFCEKRDEKVRSIDALRKKLFK 388

BLAST of Csor.00g068280 vs. TAIR 10
Match: AT1G22950.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 477.6 bits (1228), Expect = 9.2e-135
Identity = 226/380 (59.47%), Postives = 296/380 (77.89%), Query Frame = 0

Query: 1   MSLEASLER--RKQPQAPGTGNGNGVVSPSSHSFSTHRLRLQPKEDHKSESYEDLQLEFS 60
           M+L++S ++  ++Q Q P   +GNG         +  +LR  P E+H+ E+YEDL L++S
Sbjct: 10  MALDSSGKQPEQQQQQQPRASSGNGE--------ARLKLRRTPNEEHEPENYEDLPLDYS 69

Query: 61  PLLFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPEGERNRIQRHREYRQKIISNYQPL 120
           P LF+ LER+LP  +LN  R  K  +MRD+LLRY+P+ ER R+ RH+EYR KI+S+YQ L
Sbjct: 70  PSLFTSLERYLPEQLLNSTRIDKASFMRDLLLRYSPDTERVRVLRHKEYRDKIMSSYQRL 129

Query: 121 HRELYSMHAANFFVPSFLKAINENSEESFRRIMSEPSPGIYKFEMLQPQFCEKLLSEVES 180
           H E+Y++  ++FF PSFL A +  SE +FR  M E  PGI+ FEM +PQFCE LL+EVE 
Sbjct: 130 HGEIYTLDPSSFFAPSFLGAFSRKSEPNFRSSMVESYPGIFTFEMFKPQFCEMLLAEVEH 189

Query: 181 FERWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKLMDDFIRPISRVFFPEVGGATLDS 240
            E+WV++++  IMRPNTMN +G VLDDFG ++ML KL+DDFI PI++V FPEV G +LDS
Sbjct: 190 MEKWVYDSRSTIMRPNTMNNFGVVLDDFGFDSMLQKLVDDFISPIAQVLFPEVCGTSLDS 249

Query: 241 HHGFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEI 300
           HHG++VEYG DRDV+LGFHVDDSEV+LNVCLGKQFSGGEL+FRG+RCDKHVN+++  +E+
Sbjct: 250 HHGYIVEYGKDRDVDLGFHVDDSEVSLNVCLGKQFSGGELYFRGVRCDKHVNSDSTEKEV 309

Query: 301 FDYLHVPGHAVLHRGRHRHGARATTSGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQR 360
           +DY HVPGHA+LHRGRHRHGARATTSG R NL+LWCRSS FRE+K YQ+DFS WCG C+ 
Sbjct: 310 YDYSHVPGHAILHRGRHRHGARATTSGHRANLILWCRSSTFREMKNYQRDFSGWCGGCKL 369

Query: 361 EKRERQLLSIDATKQELLRR 379
           +K+ RQ  SI+ATK+ L R+
Sbjct: 370 DKQRRQRDSINATKEILARK 381

BLAST of Csor.00g068280 vs. TAIR 10
Match: AT1G48740.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 382.1 bits (980), Expect = 5.3e-106
Identity = 183/344 (53.20%), Postives = 245/344 (71.22%), Query Frame = 0

Query: 36  RLRLQPKEDHKSESYEDLQLEFSPLLFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPE 95
           RL   P  +H S++Y DL+LE+S  + S LE++LPP ML   R+ K ++M DIL +Y   
Sbjct: 32  RLSPFPNMEHISDNYGDLELEYSSAMLSSLEKYLPPEMLTATREEKAKFMSDILRKYISR 91

Query: 96  GERNRIQRHREYRQKIISNYQPLHRELYSMHAANFFVPSFLKAINENSEESFRRIMSEPS 155
            E ++ +  + Y QKI SNYQPL RELY+     F +PSF KAI+EN++ESFRRI+SEP 
Sbjct: 92  DECSKAKWCKNYWQKIKSNYQPLSRELYNFDPELFLLPSFRKAISENTKESFRRIISEPF 151

Query: 156 PGIYKFEMLQPQFCEKLLSEVESFERWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKL 215
           PG+  F+M QP F +KL+ EVE+  +WVHET F I RP  M+KYG    DFGL+ ML +L
Sbjct: 152 PGVLVFQMFQPDFIQKLIVEVENIGKWVHETNFPIRRPYHMSKYGVAFVDFGLDIMLQQL 211

Query: 216 MDDFIRPISRVFFPEVGGATLDSHHGFVVEYGIDRDVELGFHVDDSEVTLNVCLGKQFSG 275
           M++F+ PI +VFFPE  GA  DSHHG+ +E G DRD  LG+H+DDSE+TLNVC+ KQF G
Sbjct: 212 MEEFLFPICKVFFPEECGAMFDSHHGYYIENGEDRDPPLGYHLDDSEITLNVCVRKQFEG 271

Query: 276 GELFFRGIRCDKHVNTETQPEEIFDYLHVPGHAVLHRGRHRHGARATT-SGRRVNLLLWC 335
           GE+ F G RC +H  T+ +PEE+F Y H PG A+LHRGRHRHG RA T S  R N++L C
Sbjct: 272 GEISFIGTRCLRHKRTDVKPEEVFHYCHSPGQAILHRGRHRHGPRANTPSCSRANMILCC 331

Query: 336 RSSVFRELKKYQKDFSSWCGECQREKRERQLLSIDATKQELLRR 379
           R+S+FRE++KY+KDF  WC EC  EK+E++  S+DA ++ + +R
Sbjct: 332 RNSLFREMEKYEKDFPEWCNECAHEKKEKESQSLDAKRKVIKKR 375

BLAST of Csor.00g068280 vs. TAIR 10
Match: AT1G48740.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 374.4 bits (960), Expect = 1.1e-103
Identity = 183/355 (51.55%), Postives = 245/355 (69.01%), Query Frame = 0

Query: 36  RLRLQPKEDHKSESYEDLQLEFSPLLFSMLERHLPPSMLNMARDLKLQYMRDILLRYAPE 95
           RL   P  +H S++Y DL+LE+S  + S LE++LPP ML   R+ K ++M DIL +Y   
Sbjct: 32  RLSPFPNMEHISDNYGDLELEYSSAMLSSLEKYLPPEMLTATREEKAKFMSDILRKYISR 91

Query: 96  GERNRIQRHREYRQKIISNYQPLHRELYSMHAANFFVPSFLKAINENSEESFRRIMSEPS 155
            E ++ +  + Y QKI SNYQPL RELY+     F +PSF KAI+EN++ESFRRI+SEP 
Sbjct: 92  DECSKAKWCKNYWQKIKSNYQPLSRELYNFDPELFLLPSFRKAISENTKESFRRIISEPF 151

Query: 156 PGIYKFEMLQPQFCEKLLSEVESFERWVHETKFRIMRPNTMNKYGAVLDDFGLETMLDKL 215
           PG+  F+M QP F +KL+ EVE+  +WVHET F I RP  M+KYG    DFGL+ ML +L
Sbjct: 152 PGVLVFQMFQPDFIQKLIVEVENIGKWVHETNFPIRRPYHMSKYGVAFVDFGLDIMLQQL 211

Query: 216 MDDFIRPISR------------VFFPEVGGATLDSHHGFVVEYGIDRDVELGFHVDDSEV 275
           M++F+ PI +            VFFPE  GA  DSHHG+ +E G DRD  LG+H+DDSE+
Sbjct: 212 MEEFLFPICKGKAYFLLRKTFIVFFPEECGAMFDSHHGYYIENGEDRDPPLGYHLDDSEI 271

Query: 276 TLNVCLGKQFSGGELFFRGIRCDKHVNTETQPEEIFDYLHVPGHAVLHRGRHRHGARATT 335
           TLNVC+ KQF GGE+ F G RC +H  T+ +PEE+F Y H PG A+LHRGRHRHG RA T
Sbjct: 272 TLNVCVRKQFEGGEISFIGTRCLRHKRTDVKPEEVFHYCHSPGQAILHRGRHRHGPRANT 331

Query: 336 -SGRRVNLLLWCRSSVFRELKKYQKDFSSWCGECQREKRERQLLSIDATKQELLR 378
            S  R N++L CR+S+FRE++KY+KDF  WC EC  EK+E++  S+DA ++ ++R
Sbjct: 332 PSCSRANMILCCRNSLFREMEKYEKDFPEWCNECAHEKKEKESQSLDAKRKGMIR 386

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0DO231.7e-14665.632-oxoglutarate and iron-dependent oxygenase domain-containing protein CP2 OS=Ara... [more]
Q3ED681.3e-13359.472-oxoglutarate and iron-dependent oxygenase domain-containing protein ICU11 OS=A... [more]
Q28C228.3e-4036.702-oxoglutarate and iron-dependent oxygenase domain-containing protein 2 OS=Xenop... [more]
A3KGZ28.6e-3734.872-oxoglutarate and iron-dependent oxygenase domain-containing protein 2 OS=Danio... [more]
Q6N0635.6e-3636.032-oxoglutarate and iron-dependent oxygenase domain-containing protein 2 OS=Homo ... [more]
Match NameE-valueIdentityDescription
XP_022938873.15.91e-286100.00uncharacterized PKHD-type hydroxylase At1g22950-like [Cucurbita moschata] >XP_02... [more]
XP_023550033.11.69e-28599.74uncharacterized PKHD-type hydroxylase At1g22950-like [Cucurbita pepo subsp. pepo... [more]
XP_038875561.11.05e-27997.662-oxoglutarate and iron-dependent oxygenase domain-containing protein CP2-like [... [more]
XP_004147120.14.99e-27896.88uncharacterized PKHD-type hydroxylase At1g22950 [Cucumis sativus] >KGN51525.1 hy... [more]
XP_008467170.17.09e-27896.61PREDICTED: uncharacterized PKHD-type hydroxylase At1g22950-like [Cucumis melo] >... [more]
Match NameE-valueIdentityDescription
A0A6J1JSE22.86e-286100.00uncharacterized PKHD-type hydroxylase At1g22950-like OS=Cucurbita maxima OX=3661... [more]
A0A6J1FFE12.86e-286100.00uncharacterized PKHD-type hydroxylase At1g22950-like OS=Cucurbita moschata OX=36... [more]
A0A0A0KPJ62.42e-27896.88Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
A0A5A7TWL93.43e-27896.61Putative PKHD-type hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3CSW63.43e-27896.61uncharacterized PKHD-type hydroxylase At1g22950-like OS=Cucumis melo OX=3656 GN=... [more]
Match NameE-valueIdentityDescription
AT3G18210.11.2e-14765.632-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G18210.21.2e-14765.632-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G22950.19.2e-13559.472-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G48740.25.3e-10653.202-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G48740.11.1e-10351.552-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006620Prolyl 4-hydroxylase, alpha subunitSMARTSM00702p4hccoord: 156..335
e-value: 2.1E-39
score: 146.9
NoneNo IPR availableGENE3D2.60.120.620q2cbj1_9rhob like domaincoord: 158..336
e-value: 3.0E-6
score: 29.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 13..35
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 36..50
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..50
NoneNo IPR availablePANTHERPTHR24014:SF72-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 18..380
NoneNo IPR availablePANTHERPTHR240142-OXOGLUTARATE AND IRON-DEPENDENT OXYGENASE DOMAIN-CONTAINING PROTEIN 2coord: 18..380
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 237..336
score: 10.261483

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g068280.m01Csor.00g068280.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen