Csor.00g065920 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g065920
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionpre-mRNA-splicing factor SPF27 homolog
LocationCsor_Chr13: 10368305 .. 10370257 (+)
RNA-Seq ExpressionCsor.00g065920
SyntenyCsor.00g065920
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATCAACAACAATAGCGGGGATATCCTGATGCTCGAGGCTCCACCGCAGCCATCTCAACCAGCGTGGCGCGTTTCTGCAGATGCGGAGGTAATCGACGCCTTGCCCTACATCGACGATGACTATGCGGACCCAAGGGTCAAAGCAGAGGTCGATCGATTAGTGGAGGAAGAGATGCGGCGCAGCTCCAAGCGACCTGCCGACTTTCTCAAAGACTTGCCTCCTCTTCCCAAGTTCAAATTTGAGGTAGGTTAGGCTGCAATTTCCTTTCCCGCCGGAGCTTCTTCTAGGTTACGCACTTGTAGTTCCGCTTGATTTTATAGGTTATCATGCCTGAAAGAACGAATTACTTTTTCTCACTGGTTTTATTTGATAGACAGTTACTAATGCCGAAACTGGAAGTGTTTAGAAGCTTCATCTGCTAGTTTTCTTCCAAAACATCCTCTATTTGTTTTCTCGAAAACGAGATGAAAAAATTCGGATAAAAATTAATCGTTCGACAAGAACATAGGATATGAGCACACTGCTTATCCTCAAGTCACTGTGCGTCATCCAATGAATATATGACGCGCTACTCTACATAAGTTATTTTGCCACTACATTGGATAGATCTTGAAATTATTACGAAGTGTGATAATATTAGCAATAGTCTATTCAACTGACGTAGTTTGAACTCAGGATCATCCAATGCTTGCGAGAGAGTATGAACGTGTAAGAGCTGGGAGGCCTCCAATGCCTCTCGATGTTGCTAGATATAAATTAGAGACACCGCCTCCAAATAAAAAGAATGACGAAACTGCTTGGAAGCAGACACTTCAGAGAGCTCAATGCCTACTCCAGCATCAAGTGCTGAGGTGAAGTTTAGCATGTTAAAGTTGTTCCTGGCATTTAAGCTTTGTATTTCTGGAAAACTAAATTTTATGGAGAACAGTTAGAGTTGGCTACTTGAACTCATACAGTTCTTCTAATATCTGCCACACATAGTTAACATTTCTATGCCTTTTCTTGGTTGCAGTTGGTTTCTCATATCTAGTTTGTGTACCAATGAGCCATGACTGTGAGAGGTGTAATTTATAAAATTTTGATAAGAAAAGACATAATTCAGAAGATTATACGCCCAAACATGAAATTAAACATTAGAAAGGTTTTGACGATTCATTAGGAGATAATAAGAATATGGTAATCTGATGACTGGTGATGCTGTTGTCTCATACTGCAGGCTGGAAAATTTGGAACTGATGTCAAAATATGGCCCTGATATCTGGAAGCAACACAACAAACAGTTGGAAGCACTTCTATCAAGGTTTGTTACCATATTTCTTTTTGATGTCTTATATTTTGTGTACTAATTTTCTATTTATGTTGATCAAATATGATTCCAAGTCAAATCATTTGGCTTATCTCGTTCTATAGTGTAAATATGATTTCTGAATGAAGTGAACATGAAATTTAAGTACATCTGTGATCTGACTAAAGTAGACTATATGATTGTTAATATATTGTCATGATGTTATCAGAATGCAAAAATTAGCTCAAGAACAGAATGAGAAGATTGAAAAAGTGAACCGTGAAAGGAAATTCCATCAAGTAAGTAGCATCCCTGTAATTCATATTTCAATGGGGCAGAGGTCAATTCTTTGGAATGTTTGGTTGATTATTTATGCGATGATCAGCAAAACGCTGCATATGAGCTTCATGCTTTGTCCACTCAATGGAAGGAGCTGTGCGAGAAAAATATGGAAATCCAAGTGGCATGCTCTCAAATAGAGGGTCAAATCCAGGAACTAAGGAGTGAGGCAGCAGAAAAGTAATCTTGCCTTCTTCTATTTAATCTATTTTTGTCCTACATATGTTTGGCATGTTTAGTACTGATCTGTGGGTTATGATTCCAGAGGCTGGAATTTGGAAGTCAATGTAGAGAATGGTTCTGTTTCGTTCCCAGTGTCATAA

mRNA sequence

ATGGCGATCAACAACAATAGCGGGGATATCCTGATGCTCGAGGCTCCACCGCAGCCATCTCAACCAGCGTGGCGCGTTTCTGCAGATGCGGAGGTAATCGACGCCTTGCCCTACATCGACGATGACTATGCGGACCCAAGGGTCAAAGCAGAGGTCGATCGATTAGTGGAGGAAGAGATGCGGCGCAGCTCCAAGCGACCTGCCGACTTTCTCAAAGACTTGCCTCCTCTTCCCAAGTTCAAATTTGAGGATCATCCAATGCTTGCGAGAGAGTATGAACGTGTAAGAGCTGGGAGGCCTCCAATGCCTCTCGATGTTGCTAGATATAAATTAGAGACACCGCCTCCAAATAAAAAGAATGACGAAACTGCTTGGAAGCAGACACTTCAGAGAGCTCAATGCCTACTCCAGCATCAAGTGCTGAGGCTGGAAAATTTGGAACTGATGTCAAAATATGGCCCTGATATCTGGAAGCAACACAACAAACAGTTGGAAGCACTTCTATCAAGAATGCAAAAATTAGCTCAAGAACAGAATGAGAAGATTGAAAAAGTGAACCGTGAAAGGAAATTCCATCAACAAAACGCTGCATATGAGCTTCATGCTTTGTCCACTCAATGGAAGGAGCTGTGCGAGAAAAATATGGAAATCCAAGTGGCATGCTCTCAAATAGAGGGTCAAATCCAGGAACTAAGGAGTGAGGCAGCAGAAAAAGGCTGGAATTTGGAAGTCAATGTAGAGAATGGTTCTGTTTCGTTCCCAGTGTCATAA

Coding sequence (CDS)

ATGGCGATCAACAACAATAGCGGGGATATCCTGATGCTCGAGGCTCCACCGCAGCCATCTCAACCAGCGTGGCGCGTTTCTGCAGATGCGGAGGTAATCGACGCCTTGCCCTACATCGACGATGACTATGCGGACCCAAGGGTCAAAGCAGAGGTCGATCGATTAGTGGAGGAAGAGATGCGGCGCAGCTCCAAGCGACCTGCCGACTTTCTCAAAGACTTGCCTCCTCTTCCCAAGTTCAAATTTGAGGATCATCCAATGCTTGCGAGAGAGTATGAACGTGTAAGAGCTGGGAGGCCTCCAATGCCTCTCGATGTTGCTAGATATAAATTAGAGACACCGCCTCCAAATAAAAAGAATGACGAAACTGCTTGGAAGCAGACACTTCAGAGAGCTCAATGCCTACTCCAGCATCAAGTGCTGAGGCTGGAAAATTTGGAACTGATGTCAAAATATGGCCCTGATATCTGGAAGCAACACAACAAACAGTTGGAAGCACTTCTATCAAGAATGCAAAAATTAGCTCAAGAACAGAATGAGAAGATTGAAAAAGTGAACCGTGAAAGGAAATTCCATCAACAAAACGCTGCATATGAGCTTCATGCTTTGTCCACTCAATGGAAGGAGCTGTGCGAGAAAAATATGGAAATCCAAGTGGCATGCTCTCAAATAGAGGGTCAAATCCAGGAACTAAGGAGTGAGGCAGCAGAAAAAGGCTGGAATTTGGAAGTCAATGTAGAGAATGGTTCTGTTTCGTTCCCAGTGTCATAA

Protein sequence

MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEMRRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKNDETAWKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNEKIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGWNLEVNVENGSVSFPVS
Homology
BLAST of Csor.00g065920 vs. ExPASy Swiss-Prot
Match: Q949S9 (Pre-mRNA-splicing factor SPF27 homolog OS=Arabidopsis thaliana OX=3702 GN=MOS4 PE=1 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 8.4e-89
Identity = 161/244 (65.98%), Postives = 205/244 (84.02%), Query Frame = 0

Query: 6   NSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEMRRSSK 65
           N+GD+LMLEA P+ ++P W  +A+AEVIDALPYIDDDY +P +K+EVDRLVEEEMRRSSK
Sbjct: 4   NNGDVLMLEATPEAARP-WASAANAEVIDALPYIDDDYGNPLIKSEVDRLVEEEMRRSSK 63

Query: 66  RPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDV-ARYKLETPPPNKKNDETA 125
           +PADFLKDLPPLPKF F++ P+L +EYERVRAG+PP+ +D  +RYKLE PP NK+ND+ A
Sbjct: 64  KPADFLKDLPPLPKFDFKNCPVLGKEYERVRAGKPPVRIDFESRYKLEMPPANKRNDDAA 123

Query: 126 WKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNEKIEK 185
           WKQ LQ+ Q  LQ +++ LENLELMSK GP++W+Q+N +LE  L+RMQ+LAQEQNE+IEK
Sbjct: 124 WKQYLQKNQRSLQQKLIELENLELMSKLGPELWRQNNHRLEVFLTRMQRLAQEQNEEIEK 183

Query: 186 VNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGWNLEV 245
           VNRERK+HQQ  +YEL+ALS +W++LC KNMEIQ AC+ +E QI   + EAAE+GWNLE 
Sbjct: 184 VNRERKYHQQTTSYELNALSQEWRQLCVKNMEIQSACAMLETQIDSFKKEAAERGWNLEE 243

Query: 246 NVEN 249
            +EN
Sbjct: 244 KLEN 246

BLAST of Csor.00g065920 vs. ExPASy Swiss-Prot
Match: Q6PBE2 (Pre-mRNA-splicing factor SPF27 OS=Xenopus tropicalis OX=8364 GN=bcas2 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 3.2e-27
Identity = 74/212 (34.91%), Postives = 117/212 (55.19%), Query Frame = 0

Query: 32  VIDALPYIDDDYADPRVKAEVDRLVEEEMRRSSKRPADFLKDLPPLPKFKFEDHPMLARE 91
           V+DALPY D  Y    V+     LVEEE RR   RP        P P +   +  ++  E
Sbjct: 12  VVDALPYFDQGYDAQGVREAAAALVEEETRR--YRPTKNYLSYLPTPDYSAFETEIMRNE 71

Query: 92  YERVRAGRPPMPLDVARYKLETPPPNKKNDETAWKQTLQRAQCLLQHQVLRLENLELMSK 151
           +ER+ + +P   L + RY+L  P   ++ND TAW++ +  +   L+HQ +R+ENLELMS+
Sbjct: 72  FERLSSRQPLELLSMKRYELPAPLSGQRNDITAWQECVNNSMAQLEHQAVRIENLELMSQ 131

Query: 152 YGPDIWKQHNKQLEALLSRMQKLAQEQNEKIEKVNRERKFHQQNAAYELHALSTQWKELC 211
           +G + WK +N+ L  ++   QK  Q+  +KI+ +N +RK  Q  A   L  + + W  L 
Sbjct: 132 HGCNAWKVYNENLLHMIDCAQKDLQKLRKKIQDLNWQRKNSQLTAGARLREMESTWVSLV 191

Query: 212 EKNMEIQVACSQIEGQIQELRSEAAEKGWNLE 244
            KN EI+ A  Q+E ++ +L+  + E   N+E
Sbjct: 192 SKNYEIERAIVQMENEVYQLKERSGENKENIE 221

BLAST of Csor.00g065920 vs. ExPASy Swiss-Prot
Match: Q5RKQ0 (Pre-mRNA-splicing factor SPF27 OS=Danio rerio OX=7955 GN=bcas2 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 7.0e-27
Identity = 71/206 (34.47%), Postives = 110/206 (53.40%), Query Frame = 0

Query: 33  IDALPYIDDDYADPRVKAEVDRLVEEEMRRSSKRPADFLKDLPPLPKFKFEDHPMLAREY 92
           +DALPY D  Y    V+     LVEEE RR   RP        P P F   +  ++  E+
Sbjct: 13  VDALPYFDQGYDATGVREAAAALVEEETRR--YRPTKNYLSYLPTPDFSAFETEIMRNEF 72

Query: 93  ERVRAGRPPMPLDVARYKLETPPPNKKNDETAWKQTLQRAQCLLQHQVLRLENLELMSKY 152
           ER+ A +P   L + RY+L  P   +KND TAW+  +  +   L+HQ +R+ENLELM++Y
Sbjct: 73  ERLAARQPMELLSMKRYELPAPSSGQKNDMTAWQDCVNNSMAQLEHQAVRIENLELMAQY 132

Query: 153 GPDIWKQHNKQLEALLSRMQKLAQEQNEKIEKVNRERKFHQQNAAYELHALSTQWKELCE 212
           G + WK  N  L  ++   QK  Q   ++I+ +N +RK  Q     +L  L + W  L  
Sbjct: 133 GTNAWKMSNDNLALMIENSQKELQNVRKEIQDLNWQRKNDQLAGGAKLRELESNWVSLVS 192

Query: 213 KNMEIQVACSQIEGQIQELRSEAAEK 239
           KN EI+ A  Q+E ++ +++ +  ++
Sbjct: 193 KNYEIERAIVQLENEVAQMKQQQGDE 216

BLAST of Csor.00g065920 vs. ExPASy Swiss-Prot
Match: O75934 (Pre-mRNA-splicing factor SPF27 OS=Homo sapiens OX=9606 GN=BCAS2 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 9.2e-27
Identity = 72/206 (34.95%), Postives = 113/206 (54.85%), Query Frame = 0

Query: 32  VIDALPYIDDDYADPRVKAEVDRLVEEEMRRSSKRPADFLKDLPPLPKFKFEDHPMLARE 91
           V+DALPY D  Y  P V+     LVEEE RR   RP          P +   +  ++  E
Sbjct: 12  VVDALPYFDQGYEAPGVREAAAALVEEETRR--YRPTKNYLSYLTAPDYSAFETDIMRNE 71

Query: 92  YERVRAGRPPMPLDVARYKLETPPPNKKNDETAWKQTLQRAQCLLQHQVLRLENLELMSK 151
           +ER+ A +P   L + RY+L  P   +KND TAW++ +  +   L+HQ +R+ENLELMS+
Sbjct: 72  FERLAARQPIELLSMKRYELPAPSSGQKNDITAWQECVNNSMAQLEHQAVRIENLELMSQ 131

Query: 152 YGPDIWKQHNKQLEALLSRMQKLAQEQNEKIEKVNRERKFHQQNAAYELHALSTQWKELC 211
           +G + WK +N+ L  ++   QK  Q+  + I+ +N +RK  Q  A  +L  + + W  L 
Sbjct: 132 HGCNAWKVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGSKLREMESNWVSLV 191

Query: 212 EKNMEIQVACSQIEGQIQELRSEAAE 238
            KN EI+    Q+E +I +++ +  E
Sbjct: 192 SKNYEIERTIVQLENEIYQIKQQHGE 215

BLAST of Csor.00g065920 vs. ExPASy Swiss-Prot
Match: Q9D287 (Pre-mRNA-splicing factor SPF27 OS=Mus musculus OX=10090 GN=Bcas2 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 9.2e-27
Identity = 72/206 (34.95%), Postives = 113/206 (54.85%), Query Frame = 0

Query: 32  VIDALPYIDDDYADPRVKAEVDRLVEEEMRRSSKRPADFLKDLPPLPKFKFEDHPMLARE 91
           V+DALPY D  Y  P V+     LVEEE RR   RP          P +   +  ++  E
Sbjct: 12  VVDALPYFDQGYEAPGVREAAAALVEEETRR--YRPTKNYLSYLTAPDYSAFETDIMRNE 71

Query: 92  YERVRAGRPPMPLDVARYKLETPPPNKKNDETAWKQTLQRAQCLLQHQVLRLENLELMSK 151
           +ER+ A +P   L + RY+L  P   +KND TAW++ +  +   L+HQ +R+ENLELMS+
Sbjct: 72  FERLAARQPIELLSMKRYELPAPSSGQKNDITAWQECVNNSMAQLEHQAVRIENLELMSQ 131

Query: 152 YGPDIWKQHNKQLEALLSRMQKLAQEQNEKIEKVNRERKFHQQNAAYELHALSTQWKELC 211
           +G + WK +N+ L  ++   QK  Q+  + I+ +N +RK  Q  A  +L  + + W  L 
Sbjct: 132 HGCNAWKVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGSKLREMESNWVSLV 191

Query: 212 EKNMEIQVACSQIEGQIQELRSEAAE 238
            KN EI+    Q+E +I +++ +  E
Sbjct: 192 SKNYEIERTIVQLENEIYQIKQQHGE 215

BLAST of Csor.00g065920 vs. NCBI nr
Match: KAG6584331.1 (Pre-mRNA-splicing factor SPF27-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019921.1 Pre-mRNA-splicing factor SPF27-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 500 bits (1287), Expect = 3.11e-178
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0

Query: 1   MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60
           MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM
Sbjct: 1   MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60

Query: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120
           RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN
Sbjct: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120

Query: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180
           DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE
Sbjct: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180

Query: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240
           KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW
Sbjct: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240

Query: 241 NLEVNVENGSVSFPVS 256
           NLEVNVENGSVSFPVS
Sbjct: 241 NLEVNVENGSVSFPVS 256

BLAST of Csor.00g065920 vs. NCBI nr
Match: XP_023518986.1 (pre-mRNA-splicing factor SPF27 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 499 bits (1284), Expect = 8.91e-178
Identity = 255/256 (99.61%), Postives = 256/256 (100.00%), Query Frame = 0

Query: 1   MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60
           MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM
Sbjct: 1   MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60

Query: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120
           RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN
Sbjct: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120

Query: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180
           DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE
Sbjct: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180

Query: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240
           KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW
Sbjct: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240

Query: 241 NLEVNVENGSVSFPVS 256
           NLEVNVENGSVSFP+S
Sbjct: 241 NLEVNVENGSVSFPMS 256

BLAST of Csor.00g065920 vs. NCBI nr
Match: XP_022923949.1 (pre-mRNA-splicing factor SPF27 homolog [Cucurbita moschata] >XP_022923950.1 pre-mRNA-splicing factor SPF27 homolog [Cucurbita moschata])

HSP 1 Score: 498 bits (1281), Expect = 2.55e-177
Identity = 255/256 (99.61%), Postives = 255/256 (99.61%), Query Frame = 0

Query: 1   MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60
           MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM
Sbjct: 1   MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60

Query: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120
           RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN
Sbjct: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120

Query: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180
           DETAWKQTLQRAQCLLQHQVLRLENLELMSKY PDIWKQHNKQLEALLSRMQKLAQEQNE
Sbjct: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYAPDIWKQHNKQLEALLSRMQKLAQEQNE 180

Query: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240
           KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW
Sbjct: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240

Query: 241 NLEVNVENGSVSFPVS 256
           NLEVNVENGSVSFPVS
Sbjct: 241 NLEVNVENGSVSFPVS 256

BLAST of Csor.00g065920 vs. NCBI nr
Match: XP_023001174.1 (pre-mRNA-splicing factor SPF27 homolog [Cucurbita maxima] >XP_023001176.1 pre-mRNA-splicing factor SPF27 homolog [Cucurbita maxima] >XP_023001177.1 pre-mRNA-splicing factor SPF27 homolog [Cucurbita maxima])

HSP 1 Score: 494 bits (1272), Expect = 6.02e-176
Identity = 254/256 (99.22%), Postives = 254/256 (99.22%), Query Frame = 0

Query: 1   MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60
           MAINNNSGDILMLEAPP PSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM
Sbjct: 1   MAINNNSGDILMLEAPPLPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60

Query: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120
           RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN
Sbjct: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120

Query: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180
           DETAWKQTLQRAQCLLQHQVLRLENLELMSKYG DIWKQHNKQLEALLSRMQKLAQEQNE
Sbjct: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGSDIWKQHNKQLEALLSRMQKLAQEQNE 180

Query: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240
           KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW
Sbjct: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240

Query: 241 NLEVNVENGSVSFPVS 256
           NLEVNVENGSVSFPVS
Sbjct: 241 NLEVNVENGSVSFPVS 256

BLAST of Csor.00g065920 vs. NCBI nr
Match: XP_004137495.1 (pre-mRNA-splicing factor SPF27 homolog [Cucumis sativus] >KGN64160.1 hypothetical protein Csa_014348 [Cucumis sativus])

HSP 1 Score: 484 bits (1246), Expect = 5.53e-172
Identity = 246/256 (96.09%), Postives = 252/256 (98.44%), Query Frame = 0

Query: 1   MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60
           MA NNN+GDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM
Sbjct: 1   MAANNNNGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60

Query: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120
           RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPP+PLDVARYKLETPPPNKKN
Sbjct: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPVPLDVARYKLETPPPNKKN 120

Query: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180
           DETAWKQTLQ+AQCLL+HQVLRLENL+LMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE
Sbjct: 121 DETAWKQTLQKAQCLLRHQVLRLENLDLMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180

Query: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240
           KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVAC Q+EGQIQELR EAAEKGW
Sbjct: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACFQLEGQIQELRREAAEKGW 240

Query: 241 NLEVNVENGSVSFPVS 256
           NLEVNVENGSVS PVS
Sbjct: 241 NLEVNVENGSVSLPVS 256

BLAST of Csor.00g065920 vs. ExPASy TrEMBL
Match: A0A6J1EDE4 (pre-mRNA-splicing factor SPF27 homolog OS=Cucurbita moschata OX=3662 GN=LOC111431514 PE=4 SV=1)

HSP 1 Score: 498 bits (1281), Expect = 1.24e-177
Identity = 255/256 (99.61%), Postives = 255/256 (99.61%), Query Frame = 0

Query: 1   MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60
           MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM
Sbjct: 1   MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60

Query: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120
           RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN
Sbjct: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120

Query: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180
           DETAWKQTLQRAQCLLQHQVLRLENLELMSKY PDIWKQHNKQLEALLSRMQKLAQEQNE
Sbjct: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYAPDIWKQHNKQLEALLSRMQKLAQEQNE 180

Query: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240
           KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW
Sbjct: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240

Query: 241 NLEVNVENGSVSFPVS 256
           NLEVNVENGSVSFPVS
Sbjct: 241 NLEVNVENGSVSFPVS 256

BLAST of Csor.00g065920 vs. ExPASy TrEMBL
Match: A0A6J1KLZ4 (pre-mRNA-splicing factor SPF27 homolog OS=Cucurbita maxima OX=3661 GN=LOC111495390 PE=4 SV=1)

HSP 1 Score: 494 bits (1272), Expect = 2.91e-176
Identity = 254/256 (99.22%), Postives = 254/256 (99.22%), Query Frame = 0

Query: 1   MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60
           MAINNNSGDILMLEAPP PSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM
Sbjct: 1   MAINNNSGDILMLEAPPLPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60

Query: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120
           RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN
Sbjct: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120

Query: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180
           DETAWKQTLQRAQCLLQHQVLRLENLELMSKYG DIWKQHNKQLEALLSRMQKLAQEQNE
Sbjct: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGSDIWKQHNKQLEALLSRMQKLAQEQNE 180

Query: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240
           KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW
Sbjct: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240

Query: 241 NLEVNVENGSVSFPVS 256
           NLEVNVENGSVSFPVS
Sbjct: 241 NLEVNVENGSVSFPVS 256

BLAST of Csor.00g065920 vs. ExPASy TrEMBL
Match: A0A0A0LQG2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G042680 PE=4 SV=1)

HSP 1 Score: 484 bits (1246), Expect = 2.68e-172
Identity = 246/256 (96.09%), Postives = 252/256 (98.44%), Query Frame = 0

Query: 1   MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60
           MA NNN+GDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM
Sbjct: 1   MAANNNNGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60

Query: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120
           RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPP+PLDVARYKLETPPPNKKN
Sbjct: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPVPLDVARYKLETPPPNKKN 120

Query: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180
           DETAWKQTLQ+AQCLL+HQVLRLENL+LMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE
Sbjct: 121 DETAWKQTLQKAQCLLRHQVLRLENLDLMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180

Query: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240
           KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVAC Q+EGQIQELR EAAEKGW
Sbjct: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACFQLEGQIQELRREAAEKGW 240

Query: 241 NLEVNVENGSVSFPVS 256
           NLEVNVENGSVS PVS
Sbjct: 241 NLEVNVENGSVSLPVS 256

BLAST of Csor.00g065920 vs. ExPASy TrEMBL
Match: A0A1S3CHH7 (pre-mRNA-splicing factor SPF27 homolog OS=Cucumis melo OX=3656 GN=LOC103500959 PE=4 SV=1)

HSP 1 Score: 482 bits (1240), Expect = 2.20e-171
Identity = 245/256 (95.70%), Postives = 251/256 (98.05%), Query Frame = 0

Query: 1   MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60
           MA NNN+GDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM
Sbjct: 1   MAANNNNGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60

Query: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120
           RRSSKRPADFLKDLPPLPK KFEDHPMLAREYERVRAGRPP+PLDVARYKLETPPPNKKN
Sbjct: 61  RRSSKRPADFLKDLPPLPKLKFEDHPMLAREYERVRAGRPPVPLDVARYKLETPPPNKKN 120

Query: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180
           DETAWKQTLQ+AQCLL+HQVLRLENL+LMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE
Sbjct: 121 DETAWKQTLQKAQCLLRHQVLRLENLDLMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180

Query: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240
           KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVAC Q+EGQIQELR EAAEKGW
Sbjct: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACFQLEGQIQELRREAAEKGW 240

Query: 241 NLEVNVENGSVSFPVS 256
           NLEVNVENGSVS PVS
Sbjct: 241 NLEVNVENGSVSLPVS 256

BLAST of Csor.00g065920 vs. ExPASy TrEMBL
Match: A0A6J1CB04 (pre-mRNA-splicing factor SPF27 homolog OS=Momordica charantia OX=3673 GN=LOC111009059 PE=4 SV=1)

HSP 1 Score: 469 bits (1206), Expect = 3.35e-166
Identity = 237/256 (92.58%), Postives = 246/256 (96.09%), Query Frame = 0

Query: 1   MAINNNSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60
           MA N+N+GDILMLEAPPQPSQP W V+ADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM
Sbjct: 1   MATNSNNGDILMLEAPPQPSQPTWHVAADAEVIDALPYIDDDYADPRVKAEVDRLVEEEM 60

Query: 61  RRSSKRPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDVARYKLETPPPNKKN 120
           RRS KRPADFLKDLPP+PK KFEDHP+LAREYERVRAGRPP+PLDVARYKLETPPPNKKN
Sbjct: 61  RRSFKRPADFLKDLPPVPKVKFEDHPLLAREYERVRAGRPPVPLDVARYKLETPPPNKKN 120

Query: 121 DETAWKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180
           DETAWKQTLQRAQCLL+HQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE
Sbjct: 121 DETAWKQTLQRAQCLLRHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNE 180

Query: 181 KIEKVNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGW 240
           KIEKVNRERKFHQQNAAYELHALSTQWK+LC KNMEIQVACSQIEGQIQELR EAAE GW
Sbjct: 181 KIEKVNRERKFHQQNAAYELHALSTQWKDLCHKNMEIQVACSQIEGQIQELRREAAENGW 240

Query: 241 NLEVNVENGSVSFPVS 256
           NLE N+ENGSVS PVS
Sbjct: 241 NLEANIENGSVSLPVS 256

BLAST of Csor.00g065920 vs. TAIR 10
Match: AT3G18165.1 (modifier of snc1,4 )

HSP 1 Score: 328.2 bits (840), Expect = 6.0e-90
Identity = 161/244 (65.98%), Postives = 205/244 (84.02%), Query Frame = 0

Query: 6   NSGDILMLEAPPQPSQPAWRVSADAEVIDALPYIDDDYADPRVKAEVDRLVEEEMRRSSK 65
           N+GD+LMLEA P+ ++P W  +A+AEVIDALPYIDDDY +P +K+EVDRLVEEEMRRSSK
Sbjct: 4   NNGDVLMLEATPEAARP-WASAANAEVIDALPYIDDDYGNPLIKSEVDRLVEEEMRRSSK 63

Query: 66  RPADFLKDLPPLPKFKFEDHPMLAREYERVRAGRPPMPLDV-ARYKLETPPPNKKNDETA 125
           +PADFLKDLPPLPKF F++ P+L +EYERVRAG+PP+ +D  +RYKLE PP NK+ND+ A
Sbjct: 64  KPADFLKDLPPLPKFDFKNCPVLGKEYERVRAGKPPVRIDFESRYKLEMPPANKRNDDAA 123

Query: 126 WKQTLQRAQCLLQHQVLRLENLELMSKYGPDIWKQHNKQLEALLSRMQKLAQEQNEKIEK 185
           WKQ LQ+ Q  LQ +++ LENLELMSK GP++W+Q+N +LE  L+RMQ+LAQEQNE+IEK
Sbjct: 124 WKQYLQKNQRSLQQKLIELENLELMSKLGPELWRQNNHRLEVFLTRMQRLAQEQNEEIEK 183

Query: 186 VNRERKFHQQNAAYELHALSTQWKELCEKNMEIQVACSQIEGQIQELRSEAAEKGWNLEV 245
           VNRERK+HQQ  +YEL+ALS +W++LC KNMEIQ AC+ +E QI   + EAAE+GWNLE 
Sbjct: 184 VNRERKYHQQTTSYELNALSQEWRQLCVKNMEIQSACAMLETQIDSFKKEAAERGWNLEE 243

Query: 246 NVEN 249
            +EN
Sbjct: 244 KLEN 246

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949S98.4e-8965.98Pre-mRNA-splicing factor SPF27 homolog OS=Arabidopsis thaliana OX=3702 GN=MOS4 P... [more]
Q6PBE23.2e-2734.91Pre-mRNA-splicing factor SPF27 OS=Xenopus tropicalis OX=8364 GN=bcas2 PE=2 SV=1[more]
Q5RKQ07.0e-2734.47Pre-mRNA-splicing factor SPF27 OS=Danio rerio OX=7955 GN=bcas2 PE=2 SV=1[more]
O759349.2e-2734.95Pre-mRNA-splicing factor SPF27 OS=Homo sapiens OX=9606 GN=BCAS2 PE=1 SV=1[more]
Q9D2879.2e-2734.95Pre-mRNA-splicing factor SPF27 OS=Mus musculus OX=10090 GN=Bcas2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6584331.13.11e-178100.00Pre-mRNA-splicing factor SPF27-like protein, partial [Cucurbita argyrosperma sub... [more]
XP_023518986.18.91e-17899.61pre-mRNA-splicing factor SPF27 homolog [Cucurbita pepo subsp. pepo][more]
XP_022923949.12.55e-17799.61pre-mRNA-splicing factor SPF27 homolog [Cucurbita moschata] >XP_022923950.1 pre-... [more]
XP_023001174.16.02e-17699.22pre-mRNA-splicing factor SPF27 homolog [Cucurbita maxima] >XP_023001176.1 pre-mR... [more]
XP_004137495.15.53e-17296.09pre-mRNA-splicing factor SPF27 homolog [Cucumis sativus] >KGN64160.1 hypothetica... [more]
Match NameE-valueIdentityDescription
A0A6J1EDE41.24e-17799.61pre-mRNA-splicing factor SPF27 homolog OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1KLZ42.91e-17699.22pre-mRNA-splicing factor SPF27 homolog OS=Cucurbita maxima OX=3661 GN=LOC1114953... [more]
A0A0A0LQG22.68e-17296.09Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G042680 PE=4 SV=1[more]
A0A1S3CHH72.20e-17195.70pre-mRNA-splicing factor SPF27 homolog OS=Cucumis melo OX=3656 GN=LOC103500959 P... [more]
A0A6J1CB043.35e-16692.58pre-mRNA-splicing factor SPF27 homolog OS=Momordica charantia OX=3673 GN=LOC1110... [more]
Match NameE-valueIdentityDescription
AT3G18165.16.0e-9065.98modifier of snc1,4 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 214..234
NoneNo IPR availableCOILSCoilCoilcoord: 157..195
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR13296:SF0PRE-MRNA-SPLICING FACTOR SPF27coord: 15..239
IPR008409Pre-mRNA-splicing factor SPF27PFAMPF05700BCAS2coord: 31..237
e-value: 9.8E-74
score: 247.5
IPR008409Pre-mRNA-splicing factor SPF27PANTHERPTHR13296BCAS2 PROTEINcoord: 15..239

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g065920.m01Csor.00g065920.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042742 defense response to bacterium
biological_process GO:0050832 defense response to fungus
biological_process GO:0002758 innate immune response-activating signal transduction
biological_process GO:0006397 mRNA processing
biological_process GO:0008380 RNA splicing
cellular_component GO:0071013 catalytic step 2 spliceosome
cellular_component GO:0000974 Prp19 complex