Csor.00g059980 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g059980
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptiontransmembrane protein 53
LocationCsor_Chr03: 6396723 .. 6399243 (+)
RNA-Seq ExpressionCsor.00g059980
SyntenyCsor.00g059980
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGCTCCAGTCAGGGCATTTCGTCCCTGCGTTCTTAATCGCCACCTCTTCACAAAGACAATTCCTCAATCGCGCCTCGTTTCATTTCCTGAATCTAATCGGACCGCGATTTTGCGGCAATCTTCAACCTATTTCCATTTCCCTCCTCGGAAGTGCCTCGGTCTCAAATTTTCACTCTCACTCAATCTTTCTCATTGCTCTTCCTCGTCCTTTGGATCCACTGCATCTTCTATTGGCTCCAGCGCTTTTAATTTTCTTTCTTCTTTACCCTCATTACAATCTTTGGGGTCTCAATTCGCGCCTGATTACTCCAATTCGTTTCTATCTGACTCGAACGGCAGTGCCTGGACTTGGAATCGAGCTTCAGACAGTGCAATTGGAAACAACGTGGGGGCTTTGGGGGGCGAAAAGGGAGAGGCGACTGTTGTGTTGTTAGGTTGGCTTGGGGCAAAGACTAAGCATCTGAGGAGGTACGTGGAGTGGTACAATGCGAGGGGTATCAACGCCTTGACGTTTGTGCTTGATCCGAGAGAGTTTCTTTGGTTCGCGTTGAGTCGAAAAGTTGAGCAGCGGATTTCGGATTTGGCAACTGAACTCATTTCTTGGTTATCAGATGGGGAAGAGAATCATAAGGATCGGCGCCTGATTTTTCACACTTTTAGCAACACGGGCTGGTTTGTGTATGTTGAATTATGTTATCGGTATCCCATTGCTGTTTTCTTTTCTTTTCTTTTCTTTTCTTTTTTTGTCAACATAGAATTAGCTCTGTTCCCTTTTGTTTTCTTCTGCAGATACGGAGCCATTCTTGAAATTTTGCAAGGGCGGGAAGATTTGTTGGAGAAGATTAAAGGGTGCATTGTTGACTCTGGAGGAGGGGATCCGTTGAATCCTCAGGTTTGTGAACTGATTTGTAGAAGGAGTTCTAAATTATTTACTGCGTTCTTTCAACTGATGGAAACACATCTGCATGTCAATGAACTCTTAACCATCTCTGAATTTATCATCACAATGAAGGATTTAGAGGTGAAAAAATAGTAGGAACTGGCTATTGCCCTTTAAATATCATATGATCTGAATCTCAAGCCTCCATTTCTCTCTGTTCCTTCTTTCTTGCTTTAGTTCCAAAATTGTTCAGATCAGATTGCCATGATTGTACCTGCAAATTTTCATCTTGATAATGATTATTTACCCAAGATCTCATTAAAAATGTACAGATCCCAGTTTTCTAAGGCTTCTAAATGGTTGCAAAACCACAAATTTTGGTTGAACTTTTGCTTGGTATAAACTATTGAAGCATATACAAACAACTAGTTAGGTCGTTTAAGCATTAATACAACAGTATATAACTAACAGGCTTTTATCCAATCTGAATTTGATAGCATGCTTTCGTGTTATATATTTCGCAACCGAGCAAAATACTTTTTTATAATTTGTTTCTGCCTTTCTCGTCTGATTTAGCTATAAGATAACAACCATAACAACTCAAGCCCACCACCAGTAGATATTGTCTCGTTGAGGCTTTTCCTTCCGAGACTTCCCTTCAAGATTTTAAAACGTGTTTGTTGTGGAGAGGTTTCCACACGTTTATAAAGAATGTTTCGATCCCCTCTCCAACCGATGTGGGATCTCACAATCCACCTCCCTTGGGGGCCCAGCATCCTCGCTGGCACACCATCCGCCGTCCCATTTGTAATAGTCCAAGCCTACCGCTAGTAGTTATTATCCATTTTAGCCCGTTACGTATTGCCCTTAGTCTCACGATTTTAAAACACGTCTTAAAATTGTGAGGCTGATGACGATACATAATAGGCCCCAATAGGCCCCAAAGCGGACTATATATGCTAGCAGTGGGCTTAGACTTGGGCTGTTAGAACAACTAGGATGCTTTCATCTAATCTGTTTTTCTTTTTCCATTTTATCCCATCCATAGTGGAAGATTGATATATGATTTCATCTCTACTACGAACAATGGCAGATTTGGGCAGCTGGGTTTTCTGCAGCCATTCTCAAGAAAAACAGTTCTTCTACATCTCCAATGGTTAGTGGAAAGGAAATAGACAAGAAACCTCTTGTATTGGAAACCTTTGTTCTGTCATCATTGGAGAATTTTTTCTCGGTTGCTTTGAAGTTTCCCGACGTCGACAAGTAAGCTTTCTTATTATTTGAAGACATGGATGGAAACATTTTCGCTCGAGGATCGAATGGTGTCATGTACTTATCATATGAATGCATATTAATTGTTGAGAAATGAACATTTCCTTGCAGGAGGTTGAAGAAGATTGTTTCACTTCTTACAGAGTACCAGCCTTCGTATCCTGAGCTTTATCTGTACAGTTCAGGAGATAAAGTTGTACCCTTCGAGTCAATCGAGCTTCTTATCGAGAAGAGGAAGAAGACAGGAACGAAGGTTTTGTCGTACAACTTCGGCTCGTCACCGCATGTTGACCACTACAGGACGTACCCTGATATATACTCATCACAGCTTCACAAATTCTTGGTAGAGTCTTTTGCTTCATAG

mRNA sequence

ATGGAAGCTCCAGTCAGGGCATTTCGTCCCTGCGTTCTTAATCGCCACCTCTTCACAAAGACAATTCCTCAATCGCGCCTCGTTTCATTTCCTGAATCTAATCGGACCGCGATTTTGCGGCAATCTTCAACCTATTTCCATTTCCCTCCTCGGAAGTGCCTCGGTCTCAAATTTTCACTCTCACTCAATCTTTCTCATTGCTCTTCCTCGTCCTTTGGATCCACTGCATCTTCTATTGGCTCCAGCGCTTTTAATTTTCTTTCTTCTTTACCCTCATTACAATCTTTGGGGTCTCAATTCGCGCCTGATTACTCCAATTCGTTTCTATCTGACTCGAACGGCAGTGCCTGGACTTGGAATCGAGCTTCAGACAGTGCAATTGGAAACAACGTGGGGGCTTTGGGGGGCGAAAAGGGAGAGGCGACTGTTGTGTTGTTAGGTTGGCTTGGGGCAAAGACTAAGCATCTGAGGAGGTACGTGGAGTGGTACAATGCGAGGGGTATCAACGCCTTGACGTTTGTGCTTGATCCGAGAGAGTTTCTTTGGTTCGCGTTGAGTCGAAAAGTTGAGCAGCGGATTTCGGATTTGGCAACTGAACTCATTTCTTGGTTATCAGATGGGGAAGAGAATCATAAGGATCGGCGCCTGATTTTTCACACTTTTAGCAACACGGGCTGGTTTGTATACGGAGCCATTCTTGAAATTTTGCAAGGGCGGGAAGATTTGTTGGAGAAGATTAAAGGGTGCATTGTTGACTCTGGAGGAGGGGATCCGTTGAATCCTCAGATTTGGGCAGCTGGGTTTTCTGCAGCCATTCTCAAGAAAAACAGTTCTTCTACATCTCCAATGGTTAGTGGAAAGGAAATAGACAAGAAACCTCTTGTATTGGAAACCTTTGTTCTGTCATCATTGGAGAATTTTTTCTCGGTTGCTTTGAAGTTTCCCGACGTCGACAAGAGGTTGAAGAAGATTGTTTCACTTCTTACAGAGTACCAGCCTTCGTATCCTGAGCTTTATCTGTACAGTTCAGGAGATAAAGTTGTACCCTTCGAGTCAATCGAGCTTCTTATCGAGAAGAGGAAGAAGACAGGAACGAAGGTTTTGTCGTACAACTTCGGCTCGTCACCGCATGTTGACCACTACAGGACGTACCCTGATATATACTCATCACAGCTTCACAAATTCTTGGTAGAGTCTTTTGCTTCATAG

Coding sequence (CDS)

ATGGAAGCTCCAGTCAGGGCATTTCGTCCCTGCGTTCTTAATCGCCACCTCTTCACAAAGACAATTCCTCAATCGCGCCTCGTTTCATTTCCTGAATCTAATCGGACCGCGATTTTGCGGCAATCTTCAACCTATTTCCATTTCCCTCCTCGGAAGTGCCTCGGTCTCAAATTTTCACTCTCACTCAATCTTTCTCATTGCTCTTCCTCGTCCTTTGGATCCACTGCATCTTCTATTGGCTCCAGCGCTTTTAATTTTCTTTCTTCTTTACCCTCATTACAATCTTTGGGGTCTCAATTCGCGCCTGATTACTCCAATTCGTTTCTATCTGACTCGAACGGCAGTGCCTGGACTTGGAATCGAGCTTCAGACAGTGCAATTGGAAACAACGTGGGGGCTTTGGGGGGCGAAAAGGGAGAGGCGACTGTTGTGTTGTTAGGTTGGCTTGGGGCAAAGACTAAGCATCTGAGGAGGTACGTGGAGTGGTACAATGCGAGGGGTATCAACGCCTTGACGTTTGTGCTTGATCCGAGAGAGTTTCTTTGGTTCGCGTTGAGTCGAAAAGTTGAGCAGCGGATTTCGGATTTGGCAACTGAACTCATTTCTTGGTTATCAGATGGGGAAGAGAATCATAAGGATCGGCGCCTGATTTTTCACACTTTTAGCAACACGGGCTGGTTTGTATACGGAGCCATTCTTGAAATTTTGCAAGGGCGGGAAGATTTGTTGGAGAAGATTAAAGGGTGCATTGTTGACTCTGGAGGAGGGGATCCGTTGAATCCTCAGATTTGGGCAGCTGGGTTTTCTGCAGCCATTCTCAAGAAAAACAGTTCTTCTACATCTCCAATGGTTAGTGGAAAGGAAATAGACAAGAAACCTCTTGTATTGGAAACCTTTGTTCTGTCATCATTGGAGAATTTTTTCTCGGTTGCTTTGAAGTTTCCCGACGTCGACAAGAGGTTGAAGAAGATTGTTTCACTTCTTACAGAGTACCAGCCTTCGTATCCTGAGCTTTATCTGTACAGTTCAGGAGATAAAGTTGTACCCTTCGAGTCAATCGAGCTTCTTATCGAGAAGAGGAAGAAGACAGGAACGAAGGTTTTGTCGTACAACTTCGGCTCGTCACCGCATGTTGACCACTACAGGACGTACCCTGATATATACTCATCACAGCTTCACAAATTCTTGGTAGAGTCTTTTGCTTCATAG

Protein sequence

MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPPRKCLGLKFSLSLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTWNRASDSAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREFLWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGREDLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFVLSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKRKKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS
Homology
BLAST of Csor.00g059980 vs. ExPASy Swiss-Prot
Match: Q6P2H8 (Transmembrane protein 53 OS=Homo sapiens OX=9606 GN=TMEM53 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.3e-19
Identity = 84/265 (31.70%), Postives = 122/265 (46.04%), Query Frame = 0

Query: 132 GALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREFLWFALSRKVEQ 191
           G    E  +  V+LLGW G K K+L +Y   Y+ RG   + +   P   ++F+ S  +  
Sbjct: 27  GGKEAETRQPVVILLGWGGCKDKNLAKYSAIYHKRGCIVIRYTA-PWHMVFFSESLGIPS 86

Query: 192 RISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGREDLLEKIKGCIV 251
            +  LA +L+  L D E   +   L+FH FSN G  +Y  +LE+LQ R     ++ G I 
Sbjct: 87  -LRVLAQKLLELLFDYEIEKEP--LLFHVFSNGGVMLYRYVLELLQTRRFCRLRVVGTIF 146

Query: 252 DSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFVLSSLENFFSVA 311
           DS  GD  +  + A    AAIL++ ++             + L+L  F L  +   F V 
Sbjct: 147 DSAPGD--SNLVGALRALAAILERRAAML-----------RLLLLVAFALVVV--LFHVL 206

Query: 312 LKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKRKKTGTKVLSYN 371
           L  P            L +    +PELYLYS  D+VV    IE ++E R        S +
Sbjct: 207 LA-PITALFHTHFYDRLQDAGSRWPELYLYSRADEVVLARDIERMVEARLARRVLARSVD 266

Query: 372 FGSSPHVDHYRTYPDIYSSQLHKFL 397
           F SS HV H R YP  Y+S    F+
Sbjct: 267 FVSSAHVSHLRDYPTYYTSLCVDFM 271

BLAST of Csor.00g059980 vs. ExPASy Swiss-Prot
Match: Q9D0Z3 (Transmembrane protein 53 OS=Mus musculus OX=10090 GN=Tmem53 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.2e-19
Identity = 84/269 (31.23%), Postives = 122/269 (45.35%), Query Frame = 0

Query: 129 NNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREFLWFALSRK 188
           N  G     K +  V+LLGW G + K+L +Y   Y+ RG   + +   P   ++F+ S  
Sbjct: 22  NRQGGKEAGKQQPVVILLGWGGCRDKNLAKYSAIYHKRGCIVIRYTA-PWHMVFFSESLG 81

Query: 189 VEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGREDLLE-KIK 248
           +   +  +A +L+  L D E   +   L+FH FSN G  +Y  +LE+LQ  +      + 
Sbjct: 82  IPS-LRVIAQKLLELLFDYEIEREP--LLFHVFSNAGVMLYRYVLELLQTHQRFRHLHVV 141

Query: 249 GCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFVLSSLENF 308
           G I DSG GD  +  I A    A IL++      P V       + L+L  F L  +   
Sbjct: 142 GTIFDSGPGD--SNLIGALRALATILERR-----PAV------LRLLLLAAFALVVILFH 201

Query: 309 FSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKRKKTGTKV 368
           F +A   P            L +    +PELYLYS  DKVV    +E ++E R      V
Sbjct: 202 FLLA---PFTALFHTHFYDRLQDSGSCWPELYLYSRADKVVSARDVERMVEARLAHQVMV 261

Query: 369 LSYNFGSSPHVDHYRTYPDIYSSQLHKFL 397
              +F SS HV H R YP  Y+S    F+
Sbjct: 262 RGVDFVSSAHVSHLRDYPTYYTSLCVDFM 270

BLAST of Csor.00g059980 vs. ExPASy Swiss-Prot
Match: Q2TBP5 (Transmembrane protein 53 OS=Bos taurus OX=9913 GN=TMEM53 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 9.4e-18
Identity = 76/255 (29.80%), Postives = 115/255 (45.10%), Query Frame = 0

Query: 143 VVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREFLWFALSRKVEQRISDLATELIS 202
           V+LLGW G   K+L +Y   Y+ RG   + +   P   ++F+ +  +   +  LA +L+ 
Sbjct: 38  VILLGWGGCSDKNLAKYSAIYHKRGCIVIRYTA-PWHMVFFSETLGIPS-LRVLAQKLLE 97

Query: 203 WLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGREDLLE-KIKGCIVDSGGGDPLNP 262
            L D E   +   L+FH FSN G  +Y  +LE+LQ  +     ++ G I DSG GD  + 
Sbjct: 98  LLFDYEVEKEP--LLFHVFSNAGVMLYRYVLELLQTHQRFCHLRVVGTIFDSGPGD--SN 157

Query: 263 QIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFVLSSLENFFSVALKFPDVDKRL 322
            + A    A +L+   ++              L L   V  +L  F    L  P      
Sbjct: 158 LLGALRALAVVLEHRPAA--------------LRLLLLVAFTLVAFLFHVLLAPLTALFH 217

Query: 323 KKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKRKKTGTKVLSYNFGSSPHVDHY 382
                 L +    +PELYLYS  D+VV    +E ++E R      V S +F SS HV H 
Sbjct: 218 THFYDRLLDAASRWPELYLYSRADEVVLARDVERMVEARLAHQVLVRSVDFVSSAHVSHL 272

Query: 383 RTYPDIYSSQLHKFL 397
           R YP  Y++    F+
Sbjct: 278 RDYPTYYTTLCINFM 272

BLAST of Csor.00g059980 vs. ExPASy Swiss-Prot
Match: Q6DHN0 (Transmembrane protein 53 OS=Danio rerio OX=7955 GN=tmem53 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.7e-17
Identity = 65/260 (25.00%), Postives = 127/260 (48.85%), Query Frame = 0

Query: 140 EATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREFLWFALSRKVEQRISDLATE 199
           E  V+LLGW G++ KHL +Y   YN +G   L +   P + ++ + S   ++ +   A +
Sbjct: 27  EPVVILLGWAGSRDKHLAKYSSIYNEQGCTTLRYTA-PLKTVFISESLGYKE-LRSTAHK 86

Query: 200 LISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGREDLLEK-IKGCIVDSGGGDP 259
           L+  L D E   ++  + FH FSN G+ +Y  ++E+L   +      + G +VDS  G  
Sbjct: 87  LLELLYDYEV--ENNPIFFHVFSNGGFMLYRYMVELLHSHKQFSTLCVVGTVVDSAPG-- 146

Query: 260 LNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFVLS--SLENFFSVALKFPD 319
                  +      L+   ++  P V+        ++L+ F+L+  ++  F    + +P 
Sbjct: 147 -------SQNVVGALRALKTTLGPKVN--------VLLQYFLLALFAVAVFLLRIVLYPL 206

Query: 320 VDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKRKKTGTKVLSYNFGSSP 379
                +     + E+   +P++YLYS  D+V+ +  +E +++  ++ G  V S++F +  
Sbjct: 207 TKYFHRNHYDAMMEHPAPWPQMYLYSRADRVIRYRDVEKMVKGLQEKGLMVESFDFITPA 265

Query: 380 HVDHYRTYPDIYSSQLHKFL 397
           HV  +R  P+ YS++   FL
Sbjct: 267 HVSLFRDCPEDYSNRCRTFL 265

BLAST of Csor.00g059980 vs. ExPASy Swiss-Prot
Match: Q6DJC8 (Transmembrane protein 53-B OS=Xenopus laevis OX=8355 GN=tmem53-b PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.4e-13
Identity = 67/261 (25.67%), Postives = 114/261 (43.68%), Query Frame = 0

Query: 140 EATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREFLWFALSRKVEQRISDL--- 199
           E  V+LLGW G K ++L +Y   Y+ +G   + +        W A+        S L   
Sbjct: 27  EPVVILLGWGGCKDQYLAKYSAIYHNQGCTVIKYTA-----AWNAVFISESLGFSSLRED 86

Query: 200 ATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGREDLLE-KIKGCIVDSGG 259
           A +L+  L D E       ++FH FSN G+ +Y  I+E+L     L +  + G I DS  
Sbjct: 87  AKKLLELLFDYEIEKSP--ILFHVFSNGGFMLYRYIVELLHSHCRLNKLHVVGTIFDSAP 146

Query: 260 GDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFVLSSLENFFSVALKFP 319
           G+     I +      IL+ ++++            + L L  F +  +     + L +P
Sbjct: 147 GN--RNVIGSVRALDTILRTSTNNAI----------RFLALAAFAIMVI--ILRIVL-YP 206

Query: 320 DVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKRKKTGTKVLSYNFGSS 379
                 +     + +    +P+LYLYS  D ++ +  +E +I  R++      + +FG S
Sbjct: 207 VTKFLHENHYDAMKKDSSRWPQLYLYSRADPIISYIDVESMIAARRRCCLPTEALDFGKS 265

Query: 380 PHVDHYRTYPDIYSSQLHKFL 397
            HV H+R +P  YS     FL
Sbjct: 267 EHVSHFRRFPHRYSEMCTSFL 265

BLAST of Csor.00g059980 vs. NCBI nr
Match: XP_022949776.1 (transmembrane protein 53 [Cucurbita moschata] >KAG6603966.1 Transmembrane protein 53, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034136.1 Transmembrane protein 53 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 796 bits (2055), Expect = 2.26e-290
Identity = 402/402 (100.00%), Postives = 402/402 (100.00%), Query Frame = 0

Query: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPPRKCLGLKFSL 60
           MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPPRKCLGLKFSL
Sbjct: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPPRKCLGLKFSL 60

Query: 61  SLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTWN 120
           SLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTWN
Sbjct: 61  SLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTWN 120

Query: 121 RASDSAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREF 180
           RASDSAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREF
Sbjct: 121 RASDSAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREF 180

Query: 181 LWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGRE 240
           LWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGRE
Sbjct: 181 LWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGRE 240

Query: 241 DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFV 300
           DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFV
Sbjct: 241 DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFV 300

Query: 301 LSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKR 360
           LSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKR
Sbjct: 301 LSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKR 360

Query: 361 KKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS 402
           KKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS
Sbjct: 361 KKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS 402

BLAST of Csor.00g059980 vs. NCBI nr
Match: XP_023543057.1 (transmembrane protein 53 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 793 bits (2047), Expect = 3.74e-289
Identity = 400/402 (99.50%), Postives = 402/402 (100.00%), Query Frame = 0

Query: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPPRKCLGLKFSL 60
           MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPPRKCLGLKFSL
Sbjct: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPPRKCLGLKFSL 60

Query: 61  SLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTWN 120
           SLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTWN
Sbjct: 61  SLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTWN 120

Query: 121 RASDSAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREF 180
           RAS+SAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREF
Sbjct: 121 RASESAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREF 180

Query: 181 LWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGRE 240
           LWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGR+
Sbjct: 181 LWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGRK 240

Query: 241 DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFV 300
           DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFV
Sbjct: 241 DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFV 300

Query: 301 LSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKR 360
           LSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKR
Sbjct: 301 LSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKR 360

Query: 361 KKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS 402
           KKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS
Sbjct: 361 KKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS 402

BLAST of Csor.00g059980 vs. NCBI nr
Match: XP_022978506.1 (transmembrane protein 53 [Cucurbita maxima])

HSP 1 Score: 774 bits (1998), Expect = 1.10e-281
Identity = 389/402 (96.77%), Postives = 394/402 (98.01%), Query Frame = 0

Query: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPPRKCLGLKFSL 60
           MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPE NRTAI RQSSTYFHFPPRKCLGLKFSL
Sbjct: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPEFNRTAIFRQSSTYFHFPPRKCLGLKFSL 60

Query: 61  SLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTWN 120
           SLNLSHCSSSSFGSTASSIGS+AFNFLSSLPSLQS GSQFAPDYSNSFL DSNGSAWTWN
Sbjct: 61  SLNLSHCSSSSFGSTASSIGSNAFNFLSSLPSLQSFGSQFAPDYSNSFLPDSNGSAWTWN 120

Query: 121 RASDSAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREF 180
           RASDSAIG NVGALGGEKG ATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREF
Sbjct: 121 RASDSAIGINVGALGGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREF 180

Query: 181 LWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGRE 240
           LWFALSRKVEQRISDLA ELISWLSDGEENHKDRRLIFHTFSNTGWFVYGA+LEI QGR+
Sbjct: 181 LWFALSRKVEQRISDLAAELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAVLEIFQGRK 240

Query: 241 DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFV 300
           DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFV
Sbjct: 241 DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFV 300

Query: 301 LSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKR 360
           LSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKR
Sbjct: 301 LSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKR 360

Query: 361 KKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS 402
           KKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLV+SF+S
Sbjct: 361 KKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVDSFSS 402

BLAST of Csor.00g059980 vs. NCBI nr
Match: XP_008440723.1 (PREDICTED: transmembrane protein 53 [Cucumis melo] >KAA0036219.1 transmembrane protein 53 [Cucumis melo var. makuwa])

HSP 1 Score: 683 bits (1763), Expect = 7.31e-246
Identity = 345/403 (85.61%), Postives = 368/403 (91.32%), Query Frame = 0

Query: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPP-RKCLGLKFS 60
           MEAPVRAFRPCVLNR  F+KTIPQSRLVSFPESNR+AI RQSSTYFHF P RK  G K S
Sbjct: 1   MEAPVRAFRPCVLNRGFFSKTIPQSRLVSFPESNRSAIFRQSSTYFHFSPSRKYFGSKIS 60

Query: 61  LSLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTW 120
           LSLNLSHCSSSSFGS+ASS+GS   NFLSSLPSLQS GSQ A DY +SFLS+SNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSIPSNFLSSLPSLQSFGSQLASDYLSSFLSESNGSDWTW 120

Query: 121 NRASDSAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPRE 180
           NRAS+SAIGNNVG L GEKG ATVVLLGWLGAKTKHLRRYVEWYNARGINALTF++DPRE
Sbjct: 121 NRASESAIGNNVGILQGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSRKVEQRISDLA ELISWLS+GEE+ KDR LIFHTFSNTGWF+YGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFIYGAILEILQGR 240

Query: 241 EDLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETF 300
           +DLLEKIKGCIVDSGGGDPLNPQ+WAAGFSAAILKKNSSS SPMV+G+EIDKKPL+LET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEK 360
            LSSLE FFSVALK PDVDKRL  IVS+LTE QPSYPELYLYSSGDKVVP++SIELLIEK
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVPYKSIELLIEK 360

Query: 361 RKKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS 402
           R KTG K+ SYNFG SPHVDHYRTYPDIYSSQLHKFL+ESF+S
Sbjct: 361 RMKTGRKIFSYNFGMSPHVDHYRTYPDIYSSQLHKFLIESFSS 403

BLAST of Csor.00g059980 vs. NCBI nr
Match: TYK12615.1 (transmembrane protein 53 [Cucumis melo var. makuwa])

HSP 1 Score: 682 bits (1759), Expect = 2.97e-245
Identity = 344/403 (85.36%), Postives = 367/403 (91.07%), Query Frame = 0

Query: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPP-RKCLGLKFS 60
           MEAPVRAFRPCVLNR  F+KTIPQSRLVSFPESNR+AI RQSSTYFHF P RK  G K S
Sbjct: 1   MEAPVRAFRPCVLNRGFFSKTIPQSRLVSFPESNRSAIFRQSSTYFHFSPSRKYFGSKIS 60

Query: 61  LSLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTW 120
           LSLNLSHCSSSSFGS+ASS+GS   NFLSSLPSLQS GSQ A DY +SFLS+SNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSIPSNFLSSLPSLQSFGSQLASDYLSSFLSESNGSDWTW 120

Query: 121 NRASDSAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPRE 180
           NRAS+SAIGNNVG L GEKG ATVVLLGWLGAKTKHLRRYVEWYNARGINALTF++DPRE
Sbjct: 121 NRASESAIGNNVGILQGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSRKVEQRISDLA ELISWLS+GEE+ KDR LIFHTFSNTGWF+YGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFIYGAILEILQGR 240

Query: 241 EDLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETF 300
           +DLLEKIKGCIVDSGGGDPLNPQ+WAAGFSAAILKKNSSS SPMV+G+EIDKKPL+LET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEK 360
            LSSLE FFSVALK PDVDKRL  IVS+LTE QPSYPELYLYSSGDKVVP++SIELLIEK
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVPYKSIELLIEK 360

Query: 361 RKKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS 402
           R KTG K+ SYNFG SPHVDHYRTYPDIYSSQLHKF +ESF+S
Sbjct: 361 RMKTGRKIFSYNFGMSPHVDHYRTYPDIYSSQLHKFFIESFSS 403

BLAST of Csor.00g059980 vs. ExPASy TrEMBL
Match: A0A6J1GD00 (transmembrane protein 53 OS=Cucurbita moschata OX=3662 GN=LOC111453067 PE=3 SV=1)

HSP 1 Score: 796 bits (2055), Expect = 1.09e-290
Identity = 402/402 (100.00%), Postives = 402/402 (100.00%), Query Frame = 0

Query: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPPRKCLGLKFSL 60
           MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPPRKCLGLKFSL
Sbjct: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPPRKCLGLKFSL 60

Query: 61  SLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTWN 120
           SLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTWN
Sbjct: 61  SLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTWN 120

Query: 121 RASDSAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREF 180
           RASDSAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREF
Sbjct: 121 RASDSAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREF 180

Query: 181 LWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGRE 240
           LWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGRE
Sbjct: 181 LWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGRE 240

Query: 241 DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFV 300
           DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFV
Sbjct: 241 DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFV 300

Query: 301 LSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKR 360
           LSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKR
Sbjct: 301 LSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKR 360

Query: 361 KKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS 402
           KKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS
Sbjct: 361 KKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS 402

BLAST of Csor.00g059980 vs. ExPASy TrEMBL
Match: A0A6J1IMV1 (transmembrane protein 53 OS=Cucurbita maxima OX=3661 GN=LOC111478463 PE=3 SV=1)

HSP 1 Score: 774 bits (1998), Expect = 5.33e-282
Identity = 389/402 (96.77%), Postives = 394/402 (98.01%), Query Frame = 0

Query: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPPRKCLGLKFSL 60
           MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPE NRTAI RQSSTYFHFPPRKCLGLKFSL
Sbjct: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPEFNRTAIFRQSSTYFHFPPRKCLGLKFSL 60

Query: 61  SLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTWN 120
           SLNLSHCSSSSFGSTASSIGS+AFNFLSSLPSLQS GSQFAPDYSNSFL DSNGSAWTWN
Sbjct: 61  SLNLSHCSSSSFGSTASSIGSNAFNFLSSLPSLQSFGSQFAPDYSNSFLPDSNGSAWTWN 120

Query: 121 RASDSAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREF 180
           RASDSAIG NVGALGGEKG ATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREF
Sbjct: 121 RASDSAIGINVGALGGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPREF 180

Query: 181 LWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGRE 240
           LWFALSRKVEQRISDLA ELISWLSDGEENHKDRRLIFHTFSNTGWFVYGA+LEI QGR+
Sbjct: 181 LWFALSRKVEQRISDLAAELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAVLEIFQGRK 240

Query: 241 DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFV 300
           DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFV
Sbjct: 241 DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETFV 300

Query: 301 LSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKR 360
           LSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKR
Sbjct: 301 LSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEKR 360

Query: 361 KKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS 402
           KKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLV+SF+S
Sbjct: 361 KKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVDSFSS 402

BLAST of Csor.00g059980 vs. ExPASy TrEMBL
Match: A0A5A7T4A9 (Transmembrane protein 53 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold18G00160 PE=3 SV=1)

HSP 1 Score: 683 bits (1763), Expect = 3.54e-246
Identity = 345/403 (85.61%), Postives = 368/403 (91.32%), Query Frame = 0

Query: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPP-RKCLGLKFS 60
           MEAPVRAFRPCVLNR  F+KTIPQSRLVSFPESNR+AI RQSSTYFHF P RK  G K S
Sbjct: 1   MEAPVRAFRPCVLNRGFFSKTIPQSRLVSFPESNRSAIFRQSSTYFHFSPSRKYFGSKIS 60

Query: 61  LSLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTW 120
           LSLNLSHCSSSSFGS+ASS+GS   NFLSSLPSLQS GSQ A DY +SFLS+SNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSIPSNFLSSLPSLQSFGSQLASDYLSSFLSESNGSDWTW 120

Query: 121 NRASDSAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPRE 180
           NRAS+SAIGNNVG L GEKG ATVVLLGWLGAKTKHLRRYVEWYNARGINALTF++DPRE
Sbjct: 121 NRASESAIGNNVGILQGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSRKVEQRISDLA ELISWLS+GEE+ KDR LIFHTFSNTGWF+YGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFIYGAILEILQGR 240

Query: 241 EDLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETF 300
           +DLLEKIKGCIVDSGGGDPLNPQ+WAAGFSAAILKKNSSS SPMV+G+EIDKKPL+LET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEK 360
            LSSLE FFSVALK PDVDKRL  IVS+LTE QPSYPELYLYSSGDKVVP++SIELLIEK
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVPYKSIELLIEK 360

Query: 361 RKKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS 402
           R KTG K+ SYNFG SPHVDHYRTYPDIYSSQLHKFL+ESF+S
Sbjct: 361 RMKTGRKIFSYNFGMSPHVDHYRTYPDIYSSQLHKFLIESFSS 403

BLAST of Csor.00g059980 vs. ExPASy TrEMBL
Match: A0A1S3B1C0 (transmembrane protein 53 OS=Cucumis melo OX=3656 GN=LOC103485055 PE=3 SV=1)

HSP 1 Score: 683 bits (1763), Expect = 3.54e-246
Identity = 345/403 (85.61%), Postives = 368/403 (91.32%), Query Frame = 0

Query: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPP-RKCLGLKFS 60
           MEAPVRAFRPCVLNR  F+KTIPQSRLVSFPESNR+AI RQSSTYFHF P RK  G K S
Sbjct: 1   MEAPVRAFRPCVLNRGFFSKTIPQSRLVSFPESNRSAIFRQSSTYFHFSPSRKYFGSKIS 60

Query: 61  LSLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTW 120
           LSLNLSHCSSSSFGS+ASS+GS   NFLSSLPSLQS GSQ A DY +SFLS+SNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSIPSNFLSSLPSLQSFGSQLASDYLSSFLSESNGSDWTW 120

Query: 121 NRASDSAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPRE 180
           NRAS+SAIGNNVG L GEKG ATVVLLGWLGAKTKHLRRYVEWYNARGINALTF++DPRE
Sbjct: 121 NRASESAIGNNVGILQGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSRKVEQRISDLA ELISWLS+GEE+ KDR LIFHTFSNTGWF+YGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFIYGAILEILQGR 240

Query: 241 EDLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETF 300
           +DLLEKIKGCIVDSGGGDPLNPQ+WAAGFSAAILKKNSSS SPMV+G+EIDKKPL+LET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEK 360
            LSSLE FFSVALK PDVDKRL  IVS+LTE QPSYPELYLYSSGDKVVP++SIELLIEK
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVPYKSIELLIEK 360

Query: 361 RKKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS 402
           R KTG K+ SYNFG SPHVDHYRTYPDIYSSQLHKFL+ESF+S
Sbjct: 361 RMKTGRKIFSYNFGMSPHVDHYRTYPDIYSSQLHKFLIESFSS 403

BLAST of Csor.00g059980 vs. ExPASy TrEMBL
Match: A0A5D3CM53 (Transmembrane protein 53 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002010 PE=3 SV=1)

HSP 1 Score: 682 bits (1759), Expect = 1.44e-245
Identity = 344/403 (85.36%), Postives = 367/403 (91.07%), Query Frame = 0

Query: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPP-RKCLGLKFS 60
           MEAPVRAFRPCVLNR  F+KTIPQSRLVSFPESNR+AI RQSSTYFHF P RK  G K S
Sbjct: 1   MEAPVRAFRPCVLNRGFFSKTIPQSRLVSFPESNRSAIFRQSSTYFHFSPSRKYFGSKIS 60

Query: 61  LSLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTW 120
           LSLNLSHCSSSSFGS+ASS+GS   NFLSSLPSLQS GSQ A DY +SFLS+SNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSIPSNFLSSLPSLQSFGSQLASDYLSSFLSESNGSDWTW 120

Query: 121 NRASDSAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPRE 180
           NRAS+SAIGNNVG L GEKG ATVVLLGWLGAKTKHLRRYVEWYNARGINALTF++DPRE
Sbjct: 121 NRASESAIGNNVGILQGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSRKVEQRISDLA ELISWLS+GEE+ KDR LIFHTFSNTGWF+YGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFIYGAILEILQGR 240

Query: 241 EDLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETF 300
           +DLLEKIKGCIVDSGGGDPLNPQ+WAAGFSAAILKKNSSS SPMV+G+EIDKKPL+LET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEK 360
            LSSLE FFSVALK PDVDKRL  IVS+LTE QPSYPELYLYSSGDKVVP++SIELLIEK
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVPYKSIELLIEK 360

Query: 361 RKKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFLVESFAS 402
           R KTG K+ SYNFG SPHVDHYRTYPDIYSSQLHKF +ESF+S
Sbjct: 361 RMKTGRKIFSYNFGMSPHVDHYRTYPDIYSSQLHKFFIESFSS 403

BLAST of Csor.00g059980 vs. TAIR 10
Match: AT2G18245.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 337.4 bits (864), Expect = 1.6e-92
Identity = 171/307 (55.70%), Postives = 219/307 (71.34%), Query Frame = 0

Query: 104 YSNSFLSDSNGSAWTWNRASDSAI-GNNVGALGGEKG-EATVVLLGWLGAKTKHLRRYVE 163
           ++N F S+ N     WNRA      G N    GG +G E TVVLLGWLGAK KHLRRYVE
Sbjct: 84  HANPFRSEPNDGVAVWNRAPRIVNGGGNAAIFGGNEGKETTVVLLGWLGAKAKHLRRYVE 143

Query: 164 WYNARGINALTFVLDPREFLWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTF 223
           WYN+RGINA+TF +D R+ L   L R++E+RI++   EL++W+S+ E++ +++ L+FH+F
Sbjct: 144 WYNSRGINAVTFTVDVRDLLRLDLGRRLERRIAEFGNELVNWVSEKEDDGREKCLVFHSF 203

Query: 224 SNTGWFVYGAILEILQGREDLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSS--S 283
           SNTGW VYGA+LE   GR+DL+E+IKGCI+DSGG DPL+ +IWAAGF+AAILKK SS  +
Sbjct: 204 SNTGWLVYGALLESFIGRQDLVERIKGCIIDSGGADPLDTKIWAAGFTAAILKKRSSTIT 263

Query: 284 TSPMVSGKEID------KKPLVLETFVLSSLENFFSVALKFPDVDKRLKKIVSLLTEYQP 343
           T P    KE D      K+PL +E  +LSSLE  F + L  PDV+ RL KI+  L E  P
Sbjct: 264 TEPNSPIKEEDASTPQKKEPLGIENIMLSSLEKLFPIFLNHPDVNTRLTKIIQKLYENHP 323

Query: 344 SYPELYLYSSGDKVVPFESIELLIEKRKKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLH 401
             P+LYLYSSGDKVVP  S+EL I +++K G K+ S+NF SSPHVDHYR +PD+YSSQL 
Sbjct: 324 PCPQLYLYSSGDKVVPSHSVELRIREQQKIGRKIHSFNFKSSPHVDHYRNFPDLYSSQLQ 383

BLAST of Csor.00g059980 vs. TAIR 10
Match: AT3G19970.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 238.4 bits (607), Expect = 9.8e-63
Identity = 121/290 (41.72%), Postives = 181/290 (62.41%), Query Frame = 0

Query: 116 AWTWNRASDSAIG-NNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFV 175
           ++ W+    SA+  +   +   EK    VVLLGWLG+K KHL++Y +WY ++G + +TF 
Sbjct: 137 SYRWHLPEPSAVDMSGSSSCVSEKNRTVVVLLGWLGSKQKHLKKYADWYTSKGYHVITFT 196

Query: 176 LDPREFLWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILE 235
           L   E + + +  K E+ I  L   L  WL + ++    + L+FHTFSNTGW  YGAILE
Sbjct: 197 LPMNEIMSYQVGGKAEKNIESLVNHLADWLDEEQK----KNLVFHTFSNTGWLTYGAILE 256

Query: 236 ILQGRE-DLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVS------GK 295
             Q ++  L+ ++KGCIVDS      +P +WA+GFSAA LKK+S +T    S      G 
Sbjct: 257 KFQKQDSSLMGRVKGCIVDSAPVAAADPTVWASGFSAAFLKKSSVATKGSASSSYESNGI 316

Query: 296 EIDK-KPLVLETFVLSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDK 355
            I + KP   ET +L  LE FF+V L  P V++RL  ++  L+  QPS P+LY+YSS D+
Sbjct: 317 NISQPKPAATETALLLVLEKFFAVILNLPKVNRRLADVLDTLSTAQPSCPQLYIYSSADR 376

Query: 356 VVPFESIELLIEKRKKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFL 397
           V+P E +E  I +++K G +V + NF SSPHVDH+R+ P++Y+++L+ F+
Sbjct: 377 VIPVEQVESFIVEQRKAGHEVRACNFISSPHVDHFRSNPELYTAELNHFM 422

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6P2H81.3e-1931.70Transmembrane protein 53 OS=Homo sapiens OX=9606 GN=TMEM53 PE=2 SV=1[more]
Q9D0Z32.2e-1931.23Transmembrane protein 53 OS=Mus musculus OX=10090 GN=Tmem53 PE=2 SV=1[more]
Q2TBP59.4e-1829.80Transmembrane protein 53 OS=Bos taurus OX=9913 GN=TMEM53 PE=2 SV=1[more]
Q6DHN02.7e-1725.00Transmembrane protein 53 OS=Danio rerio OX=7955 GN=tmem53 PE=2 SV=1[more]
Q6DJC81.4e-1325.67Transmembrane protein 53-B OS=Xenopus laevis OX=8355 GN=tmem53-b PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022949776.12.26e-290100.00transmembrane protein 53 [Cucurbita moschata] >KAG6603966.1 Transmembrane protei... [more]
XP_023543057.13.74e-28999.50transmembrane protein 53 [Cucurbita pepo subsp. pepo][more]
XP_022978506.11.10e-28196.77transmembrane protein 53 [Cucurbita maxima][more]
XP_008440723.17.31e-24685.61PREDICTED: transmembrane protein 53 [Cucumis melo] >KAA0036219.1 transmembrane p... [more]
TYK12615.12.97e-24585.36transmembrane protein 53 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A6J1GD001.09e-290100.00transmembrane protein 53 OS=Cucurbita moschata OX=3662 GN=LOC111453067 PE=3 SV=1[more]
A0A6J1IMV15.33e-28296.77transmembrane protein 53 OS=Cucurbita maxima OX=3661 GN=LOC111478463 PE=3 SV=1[more]
A0A5A7T4A93.54e-24685.61Transmembrane protein 53 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A1S3B1C03.54e-24685.61transmembrane protein 53 OS=Cucumis melo OX=3656 GN=LOC103485055 PE=3 SV=1[more]
A0A5D3CM531.44e-24585.36Transmembrane protein 53 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
Match NameE-valueIdentityDescription
AT2G18245.11.6e-9255.70alpha/beta-Hydrolases superfamily protein [more]
AT3G19970.19.8e-6341.72alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008547Protein of unknown function DUF829, TMEM53PFAMPF05705DUF829coord: 142..396
e-value: 6.2E-59
score: 199.8
IPR008547Protein of unknown function DUF829, TMEM53PANTHERPTHR12265UNCHARACTERIZEDcoord: 69..401
NoneNo IPR availablePANTHERPTHR12265:SF11ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 69..401
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 137..396

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g059980.m01Csor.00g059980.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0097573 glutathione oxidoreductase activity