Csor.00g059320 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g059320
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionAsparagine synthetase [glutamine-hydrolyzing]
LocationCsor_Chr03: 6686845 .. 6690664 (-)
RNA-Seq ExpressionCsor.00g059320
SyntenyCsor.00g059320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTGGAATCCTCGCAGTTCTTAGCTGTGTCGACAGCTCTCAGGCCAAGCGCTCCCGAATCATCGAGTTGTCTCGGAGGTCATTTTTCTAGCATTTCTTACATTCATGTCAATGCCAAATAAACGCTACGACTTTTGTTTTATGAGATACTCTGCACAGCTTTCTGGCAGCTGTTTTGTTCCAAATATGGTTTACTTTTGTTTTTTTTTTTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTCATTCCCTGGAGTTATTTGTTTGGAAACAGGGGATTGTTTTATATTGATTGATGGTTTTTTGTTTTGAATTGTTTGGAACTGCAGGCTGCGCCATAGAGGTCCCGACTGGAGTGGTTTGCATTGTTTTGAGAACTGTTTTCTTGCTCATCAACGATTAGCTATTGTAGACCCCACTTCCGGGGATCAACCCCTTTACAACGAAGACAAGACCATCGTTGTTACGGTAATAGTATCAAATCAACCCCGTTTTTGGTTTATTAAGAAAAAACAAAGCATGTTTCAGATATTTGATAGAAAGACCTGATATTCATATGACCATGGATCAAATCTACCATATACTGTTTGTCAGTTTAAATGATGGTGTGAACCATGTTACTAGGTTAATGGAGAGATTTACAACCACAAGGAATTGAGAGAGAAGCTCAAGGGTCACCAATTTAGAACTGGCAGTGACTGTGAAGTGATTGCACACCTTGTTAGTAGCCAACAACTTATTGAGATCATTTGATTGTTTTTAGCTTTTCTATTCGTAACGCCTATATTTTCCAGTATGAAGAATATGGAGAAGAGTTTGTGGACATGTTGGATGGCATGTTTTCCTTCGTCCTTCTTGATACTCGTACCAAAACTTTTATTGCAGCTAGGGATGCTATTGGGATCACCCCATTATATATGGGATGGGGTCTTGATGGTATGTAATTTCACATTATACATGTGATTCGTGACTTCAAGTTGCATTGAGGAAACTCTCCAATTGATAGTTGATGTTCCTTTACACGTATTACTATTTCCAGTTTTACGGATTGATCCTTGAAAATATATGTCTGCTACCTCCTTCCCAGGATCAATCTGGTTTGCTTCTGAAATGAAAGCTCTAAGTGATGATTGTGAGAGATTTGTTTCTTTTCTTCCTGGTCATATATATTCCAGCAAACAAGGTATGCATCTTCTTCCCCCAACTATGGCAGTTCCAGTTTTCATAGGAGTAATGTTATGTCCCCGACTACAGTAATTCTAGTTCATAAGGTATGCATAAGTGTAATGTTAATTCTTGATATTCATTACTAAATGGTGGATTTCAAATGTTTGTAGGAGGATTAAGAAGATGGTATAACCCTCAGTGGTACACAGAGATGGTACCATCAGGTCACTATGATCCTTTGGTTCTACGTAAGGCATTCGAGAAGGCACGTTCTGGATTTACTCAATTTTAGATTATCAGACTATCTTCTTCTTCAAGGAGTTTCTGCCATTGTCAGCGTTTGATGCTTACTATTTTATAAGGAACTTTAAATCTAAAACTGGCTGCTTTTTGGTTTCTAAATGTATCTATTTTAATTGACGAGTCATTGGAATGTCTTATATTCTAAGCTTTCCTACTGTACTTTGATTGTGTAGGCTGTTACTAAAAGACTCATGACAGATGTGCCATTTGGAGTCCTTTTATCTGGAGGATTGGATTCATCACTGGTTGCTGCTGTTGCCTGCCGGCATTTGGCTGATTCTGAAGCAGCTAATCAGTGGGGGTCACAATTACACACCTTCTGTGTTGGATTGAAGGTAAGATAACGAGGCAAGTAAGGTTGTTTTTTTTATTGCCTCTGTAAATGTTGTAGAACTAACCCCTATTGAGCGAAGTCATATTTCTTTCTTTTATCGTATTCAGGGGTCCCCTGATCTTGGTGCTGCTAGAGAGGTAGCTGATTACCTTGGAACTCGTCATCATGAGTTCCACTTTACCGTGCAGGTAATTACTTTTTCTGGGATCAATTGAAAATCCATTTTGAAATGTTCACCACCAAATAATGTGTTTTTGCTTTGCTTTTTCATCCATATCAGGAAGGTATAGATGCGCTTGAGGAAGTGATTTACCACATTGAGACATACGATGTGACCACTATCAGAGCTAGCACACCTATGTTCCTCATGTCCCGGAAAATCAAATCTTTGGGAGTGAAAATGGTTCTTTCTGGCGAAGGTTCAGATGAAATTTTTGGAGGTTACTTGTACTTTCATAAGGCTCCAAACAAGAAAGAGTTTCATGAAGAAACATGCAGAAAGGTATCCTTTTCCCTCTTTTTCAACATTTTTGAGGTTACTGTTTCTTTTTATTTGTTCTAATTTATTTTCTTCTGATGTTATCAGATTAAAGCTCTTCATTTATACGACTGTTTGAGGGCCAACAAATCAACCTCAGCATGGGGTGTCGAGGCTCGTGTCCCTTTTCTTGATAAAGAATTTATAAGTACAGCCATGAACATAGATCCTGATTGGAAAATGGTACATTTTGTTTGTTTATTACACTTTGCATGTTCTTTTTTTGGTATTCAATTCTTCAACTCATTTCATCTCTCTATCAGAAAGCTCTCTTGTACATGTTTTGTTGGAACAATATCATCAATGATTAAAGTTTATGACACTAGTTACTTGCTATGAATTTATAACCGCAGATCAGGCCTGATCTTGGGAGAATAGAGAAGTGGGTCTTGCGCAATGCATTTGACGATGAGAAGGAACCATATTTGCCCAAGGTAGCTTCTGTCTTTTCTATGTGTTCAACAGAGCAGCCTGAAATCTATGCTTTCTTTTGAAATCATGGCATCGAATCATCATTTGTTCTTTACGCAAGGAAGAATATTGAATATGCCCAACCCCTCCCCTCCCCTCCCTTCTTTATAGCACATATTATACAGGCAAAAAGAACAATTCAGTGACGGTGTTGGATACAGTTGGATTGACGGTTTGAAGGACCACGCAGAGAAACAAGTAAGCTTTATCTTAATCTGTCTCACTATTGTAGCAGTTGAAATGTTCATATGGTTTTTGCTCACATGCTTTGTCCTCATCAGGTGACTGATGCGATGTTGATGAATGCGAACTTTATCTACCCAGAGAATACTCCTGCTACAAAAGAAGCATATTATTACCGGACAATATTTGAGAAATTCTTCCCTAAGGTGCTCTTTTTCTTGTTTTTTACGATATGTTTGTAGTGTGAATTTTGACGGCCTTTGACACACTTTATCCACTAATCAAACAAAACCCTGATGCATTAAATTCTTAATTTACAACAGAATGCTGCTAGATCAACGGTCCCAGGAGGTCCAAGTGTGGCATGCAGTACTGCAAAAGCAGTGGAATGGGATGCCGCATGGTCGAAAAATCTGGATCCCTCTGGTCGTGCTGCATTGGGTGTTCATGTAGCTGCGTATGAGCAAAAGCTGAATGGTAAAATTGCGAATGCACCGAATGACTCTCCCCAAGAAATCCAGTAACAGTCGAGGAAGCTGCGGCCATAGCACAGTTCAAGTTCAGGCCTAGTTTCTGATATCCTCATGTCAAGAATGGAGTTCATTCTTGAAGGAAAGAGGTAATATTGCTTTTAGTCCTTTCCAGATATCCACCATTTCCTATTCTACAATATTCCGGAACTGAATGTTGATGAAATATTGCCTTTTGAAGTTCTAATTCATTTTACTAATTGTGTTTGAACAGAGTTTTGAAATTTCCTGGAAGACAAAGGTGGGAATCTGGTTTTTCACCGTACAAATGTGGACTACATTTCCTGTAG

mRNA sequence

ATGTGTGGAATCCTCGCAGTTCTTAGCTGTGTCGACAGCTCTCAGGCCAAGCGCTCCCGAATCATCGAGTTGTCTCGGAGGCTGCGCCATAGAGGTCCCGACTGGAGTGGTTTGCATTGTTTTGAGAACTGTTTTCTTGCTCATCAACGATTAGCTATTGTAGACCCCACTTCCGGGGATCAACCCCTTTACAACGAAGACAAGACCATCGTTGTTACGGTTAATGGAGAGATTTACAACCACAAGGAATTGAGAGAGAAGCTCAAGGGTCACCAATTTAGAACTGGCAGTGACTGTGAAGTGATTGCACACCTTTATGAAGAATATGGAGAAGAGTTTGTGGACATGTTGGATGGCATGTTTTCCTTCGTCCTTCTTGATACTCGTACCAAAACTTTTATTGCAGCTAGGGATGCTATTGGGATCACCCCATTATATATGGGATGGGGTCTTGATGGATCAATCTGGTTTGCTTCTGAAATGAAAGCTCTAAGTGATGATTGTGAGAGATTTGTTTCTTTTCTTCCTGGTCATATATATTCCAGCAAACAAGGAGGATTAAGAAGATGGTATAACCCTCAGTGGTACACAGAGATGGTACCATCAGGTCACTATGATCCTTTGGTTCTACGTAAGGCATTCGAGAAGGCTGTTACTAAAAGACTCATGACAGATGTGCCATTTGGAGTCCTTTTATCTGGAGGATTGGATTCATCACTGGTTGCTGCTGTTGCCTGCCGGCATTTGGCTGATTCTGAAGCAGCTAATCAGTGGGGGTCACAATTACACACCTTCTGTGTTGGATTGAAGGGGTCCCCTGATCTTGGTGCTGCTAGAGAGGTAGCTGATTACCTTGGAACTCGTCATCATGAGTTCCACTTTACCGTGCAGGAAGGTATAGATGCGCTTGAGGAAGTGATTTACCACATTGAGACATACGATGTGACCACTATCAGAGCTAGCACACCTATGTTCCTCATGTCCCGGAAAATCAAATCTTTGGGAGTGAAAATGGTTCTTTCTGGCGAAGGTTCAGATGAAATTTTTGGAGGTTACTTGTACTTTCATAAGGCTCCAAACAAGAAAGAGTTTCATGAAGAAACATGCAGAAAGATTAAAGCTCTTCATTTATACGACTGTTTGAGGGCCAACAAATCAACCTCAGCATGGGGTGTCGAGGCTCGTGTCCCTTTTCTTGATAAAGAATTTATAAGTACAGCCATGAACATAGATCCTGATTGGAAAATGATCAGGCCTGATCTTGGGAGAATAGAGAAGTGGGTCTTGCGCAATGCATTTGACGATGAGAAGGAACCATATTTGCCCAAGCACATATTATACAGGCAAAAAGAACAATTCAGTGACGGTGTTGGATACAGTTGGATTGACGGTTTGAAGGACCACGCAGAGAAACAAGTGACTGATGCGATGTTGATGAATGCGAACTTTATCTACCCAGAGAATACTCCTGCTACAAAAGAAGCATATTATTACCGGACAATATTTGAGAAATTCTTCCCTAAGAATGCTGCTAGATCAACGGTCCCAGGAGGTCCAAGTGTGGCATGCAGTACTGCAAAAGCAGTGGAATGGGATGCCGCATGGTCGAAAAATCTGGATCCCTCTGGTCGTGCTGCATTGGGTGTTCATGTAGCTGCGTATGAGCAAAAGCTGAATGGCCTAGTTTCTGATATCCTCATGTCAAGAATGGAGTTCATTCTTGAAGGAAAGAGAGTTTTGAAATTTCCTGGAAGACAAAGGTGGGAATCTGGTTTTTCACCGTACAAATGTGGACTACATTTCCTGTAG

Coding sequence (CDS)

ATGTGTGGAATCCTCGCAGTTCTTAGCTGTGTCGACAGCTCTCAGGCCAAGCGCTCCCGAATCATCGAGTTGTCTCGGAGGCTGCGCCATAGAGGTCCCGACTGGAGTGGTTTGCATTGTTTTGAGAACTGTTTTCTTGCTCATCAACGATTAGCTATTGTAGACCCCACTTCCGGGGATCAACCCCTTTACAACGAAGACAAGACCATCGTTGTTACGGTTAATGGAGAGATTTACAACCACAAGGAATTGAGAGAGAAGCTCAAGGGTCACCAATTTAGAACTGGCAGTGACTGTGAAGTGATTGCACACCTTTATGAAGAATATGGAGAAGAGTTTGTGGACATGTTGGATGGCATGTTTTCCTTCGTCCTTCTTGATACTCGTACCAAAACTTTTATTGCAGCTAGGGATGCTATTGGGATCACCCCATTATATATGGGATGGGGTCTTGATGGATCAATCTGGTTTGCTTCTGAAATGAAAGCTCTAAGTGATGATTGTGAGAGATTTGTTTCTTTTCTTCCTGGTCATATATATTCCAGCAAACAAGGAGGATTAAGAAGATGGTATAACCCTCAGTGGTACACAGAGATGGTACCATCAGGTCACTATGATCCTTTGGTTCTACGTAAGGCATTCGAGAAGGCTGTTACTAAAAGACTCATGACAGATGTGCCATTTGGAGTCCTTTTATCTGGAGGATTGGATTCATCACTGGTTGCTGCTGTTGCCTGCCGGCATTTGGCTGATTCTGAAGCAGCTAATCAGTGGGGGTCACAATTACACACCTTCTGTGTTGGATTGAAGGGGTCCCCTGATCTTGGTGCTGCTAGAGAGGTAGCTGATTACCTTGGAACTCGTCATCATGAGTTCCACTTTACCGTGCAGGAAGGTATAGATGCGCTTGAGGAAGTGATTTACCACATTGAGACATACGATGTGACCACTATCAGAGCTAGCACACCTATGTTCCTCATGTCCCGGAAAATCAAATCTTTGGGAGTGAAAATGGTTCTTTCTGGCGAAGGTTCAGATGAAATTTTTGGAGGTTACTTGTACTTTCATAAGGCTCCAAACAAGAAAGAGTTTCATGAAGAAACATGCAGAAAGATTAAAGCTCTTCATTTATACGACTGTTTGAGGGCCAACAAATCAACCTCAGCATGGGGTGTCGAGGCTCGTGTCCCTTTTCTTGATAAAGAATTTATAAGTACAGCCATGAACATAGATCCTGATTGGAAAATGATCAGGCCTGATCTTGGGAGAATAGAGAAGTGGGTCTTGCGCAATGCATTTGACGATGAGAAGGAACCATATTTGCCCAAGCACATATTATACAGGCAAAAAGAACAATTCAGTGACGGTGTTGGATACAGTTGGATTGACGGTTTGAAGGACCACGCAGAGAAACAAGTGACTGATGCGATGTTGATGAATGCGAACTTTATCTACCCAGAGAATACTCCTGCTACAAAAGAAGCATATTATTACCGGACAATATTTGAGAAATTCTTCCCTAAGAATGCTGCTAGATCAACGGTCCCAGGAGGTCCAAGTGTGGCATGCAGTACTGCAAAAGCAGTGGAATGGGATGCCGCATGGTCGAAAAATCTGGATCCCTCTGGTCGTGCTGCATTGGGTGTTCATGTAGCTGCGTATGAGCAAAAGCTGAATGGCCTAGTTTCTGATATCCTCATGTCAAGAATGGAGTTCATTCTTGAAGGAAAGAGAGTTTTGAAATTTCCTGGAAGACAAAGGTGGGAATCTGGTTTTTCACCGTACAAATGTGGACTACATTTCCTGTAG

Protein sequence

MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGDQPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGMFSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIYSSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSLVAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMNANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLDPSGRAALGVHVAAYEQKLNGLVSDILMSRMEFILEGKRVLKFPGRQRWESGFSPYKCGLHFL
Homology
BLAST of Csor.00g059320 vs. ExPASy Swiss-Prot
Match: Q9LV77 (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Arabidopsis thaliana OX=3702 GN=ASN2 PE=2 SV=1)

HSP 1 Score: 1048.9 bits (2711), Expect = 2.2e-305
Identity = 489/556 (87.95%), Postives = 526/556 (94.60%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVL C+D+SQAKRSRIIELSRRLRHRGPDWSGLHC+E+C+LAH+RLAI+DPTSGD
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKT+ VTVNGEIYNHK LREKLK HQFRTGSDCEVIAHLYEE+GEEF+DMLDGM
Sbjct: 61  QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           F+FVLLDTR K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+SF PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSKQGGLRRWYNP WY E VPS  YDPLVLR AFEKAV KRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VAAVA RHL  SEAA QWGSQLHTFC+GL+GSPDL A REVADYLGTRHHEF FTVQ+GI
Sbjct: 241 VAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFTVQDGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DA+EEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEI GGYLYFHKAPN
Sbjct: 301 DAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALH +DCLRANKSTSAWGVEARVPFLDKEF++ AM+IDP+WK+I+PD
Sbjct: 361 KKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVAMSIDPEWKLIKPD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           LGRIEKWVLRNAFDDE+ PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA K V+D ML N
Sbjct: 421 LGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSDTMLSN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           A+F++P+NTP TKEAYYYRTIFEKFFPK+AAR+TVPGGPS+ACSTAKAVEWDA WSKNLD
Sbjct: 481 ASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSIACSTAKAVEWDATWSKNLD 540

Query: 541 PSGRAALGVHVAAYEQ 557
           PSGRAALGVHVAAYE+
Sbjct: 541 PSGRAALGVHVAAYEE 556

BLAST of Csor.00g059320 vs. ExPASy Swiss-Prot
Match: Q9LFU1 (Asparagine synthetase [glutamine-hydrolyzing] 3 OS=Arabidopsis thaliana OX=3702 GN=ASN3 PE=2 SV=1)

HSP 1 Score: 1047.7 bits (2708), Expect = 4.9e-305
Identity = 491/554 (88.63%), Postives = 526/554 (94.95%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVL CVD+SQAKRSRIIELSRRLRHRGPDWSGLHC+E+C+LAH+RLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTI VTVNGEIYNHK LRE LK HQFRTGSDCEVIAHLYEE+GEEFVDMLDGM
Sbjct: 61  QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLYEEHGEEFVDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           F+FVLLDTR K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+ F PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSKQGGLRRWYNP W++E+VPS  YDPLV+R  FEKAV KRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VA+VA RHL  SEAA QWGS+LHTFC+GLKGSPDL A REVADYLGTRHHE HFTVQ+GI
Sbjct: 241 VASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFTVQDGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DA+EEVIYH+ETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN
Sbjct: 301 DAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALH YDCLRANKSTSAWGVEARVPFLDKEFI+ AM+IDP+WKMIRPD
Sbjct: 361 KKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWKMIRPD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           LGRIEKWVLRNAFDDEK PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA K V++ MLMN
Sbjct: 421 LGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSETMLMN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           A+F++P+NTP TKEAYYYRTIFEKFFPK+AAR+TVPGGPSVACSTAKAVEWDAAWS+NLD
Sbjct: 481 ASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAKAVEWDAAWSQNLD 540

Query: 541 PSGRAALGVHVAAY 555
           PSGRAALGVHV+AY
Sbjct: 541 PSGRAALGVHVSAY 554

BLAST of Csor.00g059320 vs. ExPASy Swiss-Prot
Match: Q43011 (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0265000 PE=2 SV=3)

HSP 1 Score: 1024.6 bits (2648), Expect = 4.5e-298
Identity = 477/558 (85.48%), Postives = 523/558 (93.73%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVL   D S AKRSRIIELSRRLRHRGPDWSG+HC+++C+LAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLGVADVSLAKRSRIIELSRRLRHRGPDWSGIHCYQDCYLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDK++VVTVNGEIYNH+EL+  LK H+F+T SDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKSVVVTVNGEIYNHEELKANLKSHKFQTASDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           F+FVLLDTR K+FIAARDAIGI PLYMGWGLDGS+WF+SEMKALSDDCERF+SF PGH+Y
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGICPLYMGWGLDGSVWFSSEMKALSDDCERFISFPPGHLY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSK GGLRRWYNP W++E +PS  Y+PL+LR++FEKA+ KRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKTGGLRRWYNPPWFSESIPSTPYNPLLLRQSFEKAIIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VA+V  RHLA+++ A QWG++LHTFC+GLKGSPDL AA+EVADYLGT HHE HFTVQEGI
Sbjct: 241 VASVVSRHLAEAKVAAQWGNKLHTFCIGLKGSPDLRAAKEVADYLGTVHHELHFTVQEGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DALEEVIYH+ETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN
Sbjct: 301 DALEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDK FI+ AM+IDP+WKMI+ D
Sbjct: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKNFINVAMDIDPEWKMIKRD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           LGRIEKWVLRNAFDDE++PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA + V+D+M+MN
Sbjct: 421 LGRIEKWVLRNAFDDEEKPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANEHVSDSMMMN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           A+F+YPENTP TKEAYYYRTIFEKFFPKNAAR TVPGGPSVACSTAKAVEWDAAWSKNLD
Sbjct: 481 ASFVYPENTPVTKEAYYYRTIFEKFFPKNAARLTVPGGPSVACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVAAYEQKL 559
           PSGRAALGVH AAYE  L
Sbjct: 541 PSGRAALGVHDAAYEDTL 558

BLAST of Csor.00g059320 vs. ExPASy Swiss-Prot
Match: P31752 (Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis OX=4686 PE=2 SV=2)

HSP 1 Score: 965.3 bits (2494), Expect = 3.2e-280
Identity = 449/563 (79.75%), Postives = 502/563 (89.17%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVL C D SQAKR R++ELSRRL+HRGPDWSGL    +CFL+HQRLAI+DP SGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLCQHGDCFLSHQRLAIIDPASGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDK+IVVTVNGEIYNH+ELR +L  H++RTGSDCEVIAHLYEE+GE+FVDMLDGM
Sbjct: 61  QPLYNEDKSIVVTVNGEIYNHEELRRRLPDHKYRTGSDCEVIAHLYEEHGEDFVDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           FSFVLLDTR   F+AARDA+GITPLY+GWGLDGS+W +SEMK L+DDCE F  F PG++Y
Sbjct: 121 FSFVLLDTRNNCFVAARDAVGITPLYIGWGLDGSVWLSSEMKGLNDDCEHFEVFPPGNLY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SS+ G  RRWYNPQWY E +PS  YDPLVLRKAFE AV KRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSRSGSFRRWYNPQWYNETIPSAPYDPLVLRKAFEDAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VAAV  RHLA S+AA QWG+QLH+FCVGL+GSPDL AA+EVA+YLGT HHEFHFTVQ+GI
Sbjct: 241 VAAVTARHLAGSKAAEQWGTQLHSFCVGLEGSPDLKAAKEVAEYLGTVHHEFHFTVQDGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DA+E+VI+HIETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEGSDEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIFHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGSDEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           K+EFH ETCRKIKALH YDCLRANK+TSAWG+EARVPFLDKEF+  AM+IDP+ KMI+PD
Sbjct: 361 KEEFHHETCRKIKALHQYDCLRANKATSAWGLEARVPFLDKEFMDVAMSIDPESKMIKPD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           LGRIEKWVLR AFDDE+ PYLPKHILYRQKEQFSDGVGYSWIDGLK HA K VTD M++N
Sbjct: 421 LGRIEKWVLRKAFDDEENPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAAKHVTDRMMLN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           A  IYP NTP TKEAYYYR IFE+FFP+N+AR TVPGGPS+ACSTAKA+EWDA WS NLD
Sbjct: 481 AARIYPHNTPTTKEAYYYRMIFERFFPQNSARFTVPGGPSIACSTAKAIEWDARWSNNLD 540

Query: 541 PSGRAALGVHVAAYEQKLNGLVS 564
           PSGRAALGVH +AY+  L   +S
Sbjct: 541 PSGRAALGVHDSAYDPPLPSSIS 563

BLAST of Csor.00g059320 vs. ExPASy Swiss-Prot
Match: P49092 (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus OX=34305 GN=AS1 PE=2 SV=2)

HSP 1 Score: 950.7 bits (2456), Expect = 8.2e-276
Identity = 440/564 (78.01%), Postives = 504/564 (89.36%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVL C D +QAKR R++ELSRRL+HRGPDWSGLH   +C+LAHQRLAIVDP SGD
Sbjct: 1   MCGILAVLGCSDFTQAKRVRVLELSRRLKHRGPDWSGLHQHGDCYLAHQRLAIVDPASGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPL+NEDK+I+VTVNGEIYNH+ELR++L  HQFRTGSDC+VIAHLYEE+GE F+DMLDG+
Sbjct: 61  QPLFNEDKSIIVTVNGEIYNHEELRKQLPNHQFRTGSDCDVIAHLYEEHGENFMDMLDGI 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           FSFVLLDTR  TFI ARDAIG+T LY+GWGLDGS+W +SEMK L+DDCE F  F PGH+Y
Sbjct: 121 FSFVLLDTRDNTFIVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFEVFPPGHLY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SS++   RRWYNP W++E +PS  YDPL +R AFEKAV KRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSRERAFRRWYNPTWFSESIPSAPYDPLAVRHAFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VA++  R+LA ++AA QWGS+LH+FCVGL+GSPDL AA+EVADYLGT HHEF FTVQ+GI
Sbjct: 241 VASITSRYLATTKAAEQWGSKLHSFCVGLEGSPDLKAAKEVADYLGTVHHEFTFTVQDGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DA+EEVIYH+ETYDVTTIRASTPMFLMSRKIKSLGVK V+SGEGSDEIFGGYLYFHKAPN
Sbjct: 301 DAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKWVISGEGSDEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           K+EFH ETCRKIKALH YDCLRANKST AWG+EARVPFLDKEFI+ AMNIDP++KMI+ D
Sbjct: 361 KEEFHTETCRKIKALHQYDCLRANKSTFAWGLEARVPFLDKEFINVAMNIDPEYKMIKRD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
            GRIEK++LR AFDDE++PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA K VTD M++N
Sbjct: 421 EGRIEKYILRRAFDDEEKPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAKHVTDKMILN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           A  I+  NTP TKEAYYYR IFE+FFP+N+AR TVPGGP+VACSTAKAVEWDAAWS NLD
Sbjct: 481 AGNIFRHNTPLTKEAYYYRMIFERFFPQNSARLTVPGGPTVACSTAKAVEWDAAWSNNLD 540

Query: 541 PSGRAALGVHVAAYEQKLNGLVSD 565
           PSGRAALGVH++AY+ K N L+++
Sbjct: 541 PSGRAALGVHLSAYDDKQNNLINN 564

BLAST of Csor.00g059320 vs. NCBI nr
Match: KAG6604032.1 (Asparagine synthetase [glutamine-hydrolyzing] 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1248 bits (3230), Expect = 0.0
Identity = 602/602 (100.00%), Postives = 602/602 (100.00%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI
Sbjct: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN
Sbjct: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD
Sbjct: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN
Sbjct: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD
Sbjct: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVAAYEQKLNGLVSDILMSRMEFILEGKRVLKFPGRQRWESGFSPYKCGLH 600
           PSGRAALGVHVAAYEQKLNGLVSDILMSRMEFILEGKRVLKFPGRQRWESGFSPYKCGLH
Sbjct: 541 PSGRAALGVHVAAYEQKLNGLVSDILMSRMEFILEGKRVLKFPGRQRWESGFSPYKCGLH 600

Query: 601 FL 602
           FL
Sbjct: 601 FL 602

BLAST of Csor.00g059320 vs. NCBI nr
Match: XP_022950083.1 (asparagine synthetase [glutamine-hydrolyzing] 2 [Cucurbita moschata])

HSP 1 Score: 1156 bits (2991), Expect = 0.0
Identity = 558/564 (98.94%), Postives = 563/564 (99.82%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VAAVACR+LADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI
Sbjct: 241 VAAVACRNLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN
Sbjct: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAM+IDPDWKMIRPD
Sbjct: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMSIDPDWKMIRPD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN
Sbjct: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD
Sbjct: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVAAYEQKLNGLVSD 564
           PSGRAALGVHVAAYEQKLNG +++
Sbjct: 541 PSGRAALGVHVAAYEQKLNGKIAN 564

BLAST of Csor.00g059320 vs. NCBI nr
Match: XP_023544369.1 (asparagine synthetase [glutamine-hydrolyzing] 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1156 bits (2990), Expect = 0.0
Identity = 557/564 (98.76%), Postives = 563/564 (99.82%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTIVVTVNGEIYNHKELREKLKGH+FRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHKFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVA+YLGTRHHEFHFTVQEGI
Sbjct: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVAEYLGTRHHEFHFTVQEGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN
Sbjct: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAM+IDPDWKMIRPD
Sbjct: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMSIDPDWKMIRPD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN
Sbjct: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD
Sbjct: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVAAYEQKLNGLVSD 564
           PSGRAALGVHVAAYEQKLNG +++
Sbjct: 541 PSGRAALGVHVAAYEQKLNGKIAN 564

BLAST of Csor.00g059320 vs. NCBI nr
Match: XP_022978448.1 (asparagine synthetase [glutamine-hydrolyzing] 2 [Cucurbita maxima])

HSP 1 Score: 1153 bits (2982), Expect = 0.0
Identity = 556/564 (98.58%), Postives = 560/564 (99.29%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSKQGGLRRWYNPQWYTEMVP GHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPQWYTEMVPLGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI
Sbjct: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN
Sbjct: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALHLYDCLRANKSTSAWGVE RVPFLDKEFISTAM+IDPDWKMIRPD
Sbjct: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVETRVPFLDKEFISTAMSIDPDWKMIRPD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN
Sbjct: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD
Sbjct: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVAAYEQKLNGLVSD 564
           PSGRAALGVHVAAYEQKLN  +++
Sbjct: 541 PSGRAALGVHVAAYEQKLNAKIAN 564

BLAST of Csor.00g059320 vs. NCBI nr
Match: KAG7034197.1 (Asparagine synthetase [glutamine-hydrolyzing] 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1151 bits (2977), Expect = 0.0
Identity = 560/579 (96.72%), Postives = 563/579 (97.24%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKA---------------VTKRLMTD 240
           SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKA               VTKRLMTD
Sbjct: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKARTLNLKLAAFWFLNAVTKRLMTD 240

Query: 241 VPFGVLLSGGLDSSLVAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYL 300
           VPFGVLLSGGLDSSLVAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYL
Sbjct: 241 VPFGVLLSGGLDSSLVAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYL 300

Query: 301 GTRHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGS 360
           GTRHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGS
Sbjct: 301 GTRHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGS 360

Query: 361 DEIFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFIS 420
           DEIFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFIS
Sbjct: 361 DEIFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFIS 420

Query: 421 TAMNIDPDWKMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGL 480
           TAMNIDPDWKMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGL
Sbjct: 421 TAMNIDPDWKMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGL 480

Query: 481 KDHAEKQVTDAMLMNANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACST 540
           KDHAEKQVTDAMLMNANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACST
Sbjct: 481 KDHAEKQVTDAMLMNANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACST 540

Query: 541 AKAVEWDAAWSKNLDPSGRAALGVHVAAYEQKLNGLVSD 564
           AKAVEWDAAWSKNLDPSGRAALGVHVAAYEQKLNG +++
Sbjct: 541 AKAVEWDAAWSKNLDPSGRAALGVHVAAYEQKLNGKIAN 579

BLAST of Csor.00g059320 vs. ExPASy TrEMBL
Match: A0A6J1GDX0 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita moschata OX=3662 GN=LOC111453271 PE=4 SV=1)

HSP 1 Score: 1156 bits (2991), Expect = 0.0
Identity = 558/564 (98.94%), Postives = 563/564 (99.82%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VAAVACR+LADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI
Sbjct: 241 VAAVACRNLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN
Sbjct: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAM+IDPDWKMIRPD
Sbjct: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMSIDPDWKMIRPD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN
Sbjct: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD
Sbjct: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVAAYEQKLNGLVSD 564
           PSGRAALGVHVAAYEQKLNG +++
Sbjct: 541 PSGRAALGVHVAAYEQKLNGKIAN 564

BLAST of Csor.00g059320 vs. ExPASy TrEMBL
Match: A0A6J1IT53 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita maxima OX=3661 GN=LOC111478427 PE=4 SV=1)

HSP 1 Score: 1153 bits (2982), Expect = 0.0
Identity = 556/564 (98.58%), Postives = 560/564 (99.29%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSKQGGLRRWYNPQWYTEMVP GHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPQWYTEMVPLGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI
Sbjct: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN
Sbjct: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALHLYDCLRANKSTSAWGVE RVPFLDKEFISTAM+IDPDWKMIRPD
Sbjct: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVETRVPFLDKEFISTAMSIDPDWKMIRPD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN
Sbjct: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD
Sbjct: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVAAYEQKLNGLVSD 564
           PSGRAALGVHVAAYEQKLN  +++
Sbjct: 541 PSGRAALGVHVAAYEQKLNAKIAN 564

BLAST of Csor.00g059320 vs. ExPASy TrEMBL
Match: A0A5D3CQI6 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002840 PE=4 SV=1)

HSP 1 Score: 1128 bits (2918), Expect = 0.0
Identity = 539/565 (95.40%), Postives = 556/565 (98.41%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVL CVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENC+LAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKT+VVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSKQGGLRRWYNP W+TEMVPSG YDPLVLR AFEKAVTKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWFTEMVPSGSYDPLVLRNAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VAAVACRHLADSEAA QWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI
Sbjct: 241 VAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN
Sbjct: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALHLYDCLRANKSTSAWG+EARVPFLDKEFI+ AM+IDPDWKMIR D
Sbjct: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFINIAMSIDPDWKMIRRD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           +GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD+ML+N
Sbjct: 421 IGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDSMLVN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           ANFIYPENTP TKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD
Sbjct: 481 ANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVAAYEQKLNGLVSDI 565
           PSGRAALGVHVAAY++KLNG ++++
Sbjct: 541 PSGRAALGVHVAAYDEKLNGKIANV 565

BLAST of Csor.00g059320 vs. ExPASy TrEMBL
Match: A0A1S3B235 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo OX=3656 GN=LOC103484971 PE=4 SV=1)

HSP 1 Score: 1128 bits (2918), Expect = 0.0
Identity = 539/565 (95.40%), Postives = 556/565 (98.41%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVL CVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENC+LAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKT+VVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSKQGGLRRWYNP W+TEMVPSG YDPLVLR AFEKAVTKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWFTEMVPSGSYDPLVLRNAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VAAVACRHLADSEAA QWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI
Sbjct: 241 VAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN
Sbjct: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALHLYDCLRANKSTSAWG+EARVPFLDKEFI+ AM+IDPDWKMIR D
Sbjct: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFINIAMSIDPDWKMIRRD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           +GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD+ML+N
Sbjct: 421 IGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDSMLVN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           ANFIYPENTP TKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD
Sbjct: 481 ANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVAAYEQKLNGLVSDI 565
           PSGRAALGVHVAAY++KLNG ++++
Sbjct: 541 PSGRAALGVHVAAYDEKLNGKIANV 565

BLAST of Csor.00g059320 vs. ExPASy TrEMBL
Match: A0A6J1BUB1 (Asparagine synthetase [glutamine-hydrolyzing] OS=Momordica charantia OX=3673 GN=LOC111005610 PE=4 SV=1)

HSP 1 Score: 1126 bits (2912), Expect = 0.0
Identity = 541/566 (95.58%), Postives = 552/566 (97.53%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVL C D SQAKRSRIIELSRRLRHRGPDWSGLHCFENC+LAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKT+VVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           FSFVLLDTR KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERF+SFLPGHIY
Sbjct: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSK+GGLRRWYNP WYTEMVPSG YD LVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDLLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VAAVACRHLADSEAA QWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI
Sbjct: 241 VAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN
Sbjct: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAM++DPDWKMIR D
Sbjct: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMSMDPDWKMIRRD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAE+QVTDAMLMN
Sbjct: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEQQVTDAMLMN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           ANF+YPENTP TKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD
Sbjct: 481 ANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVAAYEQKLNGLVSDIL 566
           PSGRAALGVHVAAYE+KLN  ++  L
Sbjct: 541 PSGRAALGVHVAAYEEKLNAKIATAL 566

BLAST of Csor.00g059320 vs. TAIR 10
Match: AT5G65010.1 (asparagine synthetase 2 )

HSP 1 Score: 1048.9 bits (2711), Expect = 1.6e-306
Identity = 489/556 (87.95%), Postives = 526/556 (94.60%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVL C+D+SQAKRSRIIELSRRLRHRGPDWSGLHC+E+C+LAH+RLAI+DPTSGD
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKT+ VTVNGEIYNHK LREKLK HQFRTGSDCEVIAHLYEE+GEEF+DMLDGM
Sbjct: 61  QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           F+FVLLDTR K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+SF PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSKQGGLRRWYNP WY E VPS  YDPLVLR AFEKAV KRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VAAVA RHL  SEAA QWGSQLHTFC+GL+GSPDL A REVADYLGTRHHEF FTVQ+GI
Sbjct: 241 VAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFTVQDGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DA+EEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEI GGYLYFHKAPN
Sbjct: 301 DAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALH +DCLRANKSTSAWGVEARVPFLDKEF++ AM+IDP+WK+I+PD
Sbjct: 361 KKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVAMSIDPEWKLIKPD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           LGRIEKWVLRNAFDDE+ PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA K V+D ML N
Sbjct: 421 LGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSDTMLSN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           A+F++P+NTP TKEAYYYRTIFEKFFPK+AAR+TVPGGPS+ACSTAKAVEWDA WSKNLD
Sbjct: 481 ASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSIACSTAKAVEWDATWSKNLD 540

Query: 541 PSGRAALGVHVAAYEQ 557
           PSGRAALGVHVAAYE+
Sbjct: 541 PSGRAALGVHVAAYEE 556

BLAST of Csor.00g059320 vs. TAIR 10
Match: AT5G10240.1 (asparagine synthetase 3 )

HSP 1 Score: 1047.7 bits (2708), Expect = 3.5e-306
Identity = 491/554 (88.63%), Postives = 526/554 (94.95%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVL CVD+SQAKRSRIIELSRRLRHRGPDWSGLHC+E+C+LAH+RLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTI VTVNGEIYNHK LRE LK HQFRTGSDCEVIAHLYEE+GEEFVDMLDGM
Sbjct: 61  QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLYEEHGEEFVDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           F+FVLLDTR K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+ F PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSKQGGLRRWYNP W++E+VPS  YDPLV+R  FEKAV KRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VA+VA RHL  SEAA QWGS+LHTFC+GLKGSPDL A REVADYLGTRHHE HFTVQ+GI
Sbjct: 241 VASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFTVQDGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DA+EEVIYH+ETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN
Sbjct: 301 DAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALH YDCLRANKSTSAWGVEARVPFLDKEFI+ AM+IDP+WKMIRPD
Sbjct: 361 KKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWKMIRPD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           LGRIEKWVLRNAFDDEK PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA K V++ MLMN
Sbjct: 421 LGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSETMLMN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           A+F++P+NTP TKEAYYYRTIFEKFFPK+AAR+TVPGGPSVACSTAKAVEWDAAWS+NLD
Sbjct: 481 ASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAKAVEWDAAWSQNLD 540

Query: 541 PSGRAALGVHVAAY 555
           PSGRAALGVHV+AY
Sbjct: 541 PSGRAALGVHVSAY 554

BLAST of Csor.00g059320 vs. TAIR 10
Match: AT5G65010.2 (asparagine synthetase 2 )

HSP 1 Score: 1044.3 bits (2699), Expect = 3.9e-305
Identity = 489/557 (87.79%), Postives = 526/557 (94.43%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVL C+D+SQAKRSRIIELSRRLRHRGPDWSGLHC+E+C+LAH+RLAI+DPTSGD
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKT+ VTVNGEIYNHK LREKLK HQFRTGSDCEVIAHLYEE+GEEF+DMLDGM
Sbjct: 61  QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           F+FVLLDTR K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+SF PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSKQGGLRRWYNP WY E VPS  YDPLVLR AFEKAV KRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VAAVA RHL  SEAA QWGSQLHTFC+GL+GSPDL A REVADYLGTRHHEF FTVQ+GI
Sbjct: 241 VAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFTVQDGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DA+EEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEI GGYLYFHKAPN
Sbjct: 301 DAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALH +DCLRANKSTSAWGVEARVPFLDKEF++ AM+IDP+WK+I+PD
Sbjct: 361 KKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVAMSIDPEWKLIKPD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           LGRIEKWVLRNAFDDE+ PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA K V+D ML N
Sbjct: 421 LGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSDTMLSN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPK-NAARSTVPGGPSVACSTAKAVEWDAAWSKNL 540
           A+F++P+NTP TKEAYYYRTIFEKFFPK +AAR+TVPGGPS+ACSTAKAVEWDA WSKNL
Sbjct: 481 ASFVFPDNTPLTKEAYYYRTIFEKFFPKQSAARATVPGGPSIACSTAKAVEWDATWSKNL 540

Query: 541 DPSGRAALGVHVAAYEQ 557
           DPSGRAALGVHVAAYE+
Sbjct: 541 DPSGRAALGVHVAAYEE 557

BLAST of Csor.00g059320 vs. TAIR 10
Match: AT5G10240.2 (asparagine synthetase 3 )

HSP 1 Score: 1036.6 bits (2679), Expect = 8.0e-303
Identity = 488/554 (88.09%), Postives = 524/554 (94.58%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVL CVD+SQAKRSRIIELSRRLRHRGPDWSGLHC+E+C+LAH+RLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTI VTVNGEIYNHK LRE LK HQFRTGSDCEVIAHL  ++GEEFVDMLDGM
Sbjct: 61  QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLV-KHGEEFVDMLDGM 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           F+FVLLDTR K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+ F PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSKQGGLRRWYNP W++E+VPS  YDPLV+R  FEKAV KRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VA+VA RHL  SEAA QWGS+LHTFC+GLKGSPDL A REVADYLGTRHHE HFTVQ+GI
Sbjct: 241 VASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFTVQDGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DA+EEVIYH+ETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN
Sbjct: 301 DAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFHEETCRKIKALH YDCLRANKSTSAWGVEARVPFLDKEFI+ AM+IDP+WKMIRPD
Sbjct: 361 KKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWKMIRPD 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
           LGRIEKWVLRNAFDDEK PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA K V++ MLMN
Sbjct: 421 LGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSETMLMN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           A+F++P+NTP TKEAYYYRTIFEKFFPK+AAR+TVPGGPSVACSTAKAVEWDAAWS+NLD
Sbjct: 481 ASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAKAVEWDAAWSQNLD 540

Query: 541 PSGRAALGVHVAAY 555
           PSGRAALGVHV+AY
Sbjct: 541 PSGRAALGVHVSAY 553

BLAST of Csor.00g059320 vs. TAIR 10
Match: AT3G47340.1 (glutamine-dependent asparagine synthase 1 )

HSP 1 Score: 940.3 bits (2429), Expect = 7.8e-274
Identity = 433/557 (77.74%), Postives = 499/557 (89.59%), Query Frame = 0

Query: 1   MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
           MCGILAVL C D SQAKR R++ELSRRLRHRGPDWSGL+   + +LAHQRLA++DP SGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGDNYLAHQRLAVIDPASGD 60

Query: 61  QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPL+NEDKTIVVTVNGEIYNH+ELR++LK H+FRTGSDCEVIAHLYEEYG +FVDMLDG+
Sbjct: 61  QPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVIAHLYEEYGVDFVDMLDGI 120

Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
           FSFVLLDTR  +F+ ARDAIG+T LY+GWGLDGS+W +SEMK L+DDCE F +F PGH Y
Sbjct: 121 FSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFETFPPGHFY 180

Query: 181 SSKQGGLRRWYNPQWYTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSGGLDSSL 240
           SSK GG ++WYNP W+ E VPS  Y+PL +R+AFE AV KRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKLGGFKQWYNPPWFNESVPSTPYEPLAIRRAFENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGI 300
           VA++  RHLA ++AA QWG QLH+FCVGL+GSPDL A +EVA+YLGT HHEFHF+VQ+GI
Sbjct: 241 VASITARHLAGTKAAKQWGPQLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHFSVQDGI 300

Query: 301 DALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360
           DA+E+VIYH+ETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEG+DEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGADEIFGGYLYFHKAPN 360

Query: 361 KKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMNIDPDWKMIRPD 420
           KKEFH+ETCRKIKALH YDCLRANKSTSA+G+EARVPFLDK+FI+TAM++DP+ KMI+P+
Sbjct: 361 KKEFHQETCRKIKALHKYDCLRANKSTSAFGLEARVPFLDKDFINTAMSLDPESKMIKPE 420

Query: 421 LGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTDAMLMN 480
            GRIEKWVLR AFDDE+ PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA + V D M+ N
Sbjct: 421 EGRIEKWVLRRAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAQNVNDKMMSN 480

Query: 481 ANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLD 540
           A  I+P NTP TKEAYYYR IFE+FFP+N+AR TVPGG +VACSTAKAVEWDA+WS N+D
Sbjct: 481 AGHIFPHNTPNTKEAYYYRMIFERFFPQNSARLTVPGGATVACSTAKAVEWDASWSNNMD 540

Query: 541 PSGRAALGVHVAAYEQK 558
           PSGRAA+GVH++AY+ K
Sbjct: 541 PSGRAAIGVHLSAYDGK 557

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LV772.2e-30587.95Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9LFU14.9e-30588.63Asparagine synthetase [glutamine-hydrolyzing] 3 OS=Arabidopsis thaliana OX=3702 ... [more]
Q430114.5e-29885.48Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Oryza sativa subsp. japonica ... [more]
P317523.2e-28079.75Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis OX=4686 P... [more]
P490928.2e-27678.01Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus OX=34305 GN=A... [more]
Match NameE-valueIdentityDescription
KAG6604032.10.0100.00Asparagine synthetase [glutamine-hydrolyzing] 3, partial [Cucurbita argyrosperma... [more]
XP_022950083.10.098.94asparagine synthetase [glutamine-hydrolyzing] 2 [Cucurbita moschata][more]
XP_023544369.10.098.76asparagine synthetase [glutamine-hydrolyzing] 2 [Cucurbita pepo subsp. pepo][more]
XP_022978448.10.098.58asparagine synthetase [glutamine-hydrolyzing] 2 [Cucurbita maxima][more]
KAG7034197.10.096.72Asparagine synthetase [glutamine-hydrolyzing] 3 [Cucurbita argyrosperma subsp. a... [more]
Match NameE-valueIdentityDescription
A0A6J1GDX00.098.94Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1IT530.098.58Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A5D3CQI60.095.40Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3B2350.095.40Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A6J1BUB10.095.58Asparagine synthetase [glutamine-hydrolyzing] OS=Momordica charantia OX=3673 GN=... [more]
Match NameE-valueIdentityDescription
AT5G65010.11.6e-30687.95asparagine synthetase 2 [more]
AT5G10240.13.5e-30688.63asparagine synthetase 3 [more]
AT5G65010.23.9e-30587.79asparagine synthetase 2 [more]
AT5G10240.28.0e-30388.09asparagine synthetase 3 [more]
AT3G47340.17.8e-27477.74glutamine-dependent asparagine synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 195..514
e-value: 3.7E-117
score: 393.4
IPR006426Asparagine synthase, glutamine-hydrolyzingTIGRFAMTIGR01536TIGR01536coord: 2..367
e-value: 4.2E-114
score: 380.3
IPR006426Asparagine synthase, glutamine-hydrolyzingPIRSFPIRSF001589Asn_synthetase_glu-hcoord: 1..361
e-value: 6.7E-114
score: 379.4
coord: 356..516
e-value: 8.4E-28
score: 95.1
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 2..192
e-value: 9.3E-64
score: 216.2
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 1..195
IPR001962Asparagine synthasePFAMPF00733Asn_synthasecoord: 210..361
e-value: 2.9E-59
score: 201.5
IPR001962Asparagine synthaseCDDcd01991Asn_Synthase_B_Ccoord: 211..456
e-value: 2.7113E-80
score: 252.34
IPR017932Glutamine amidotransferase type 2 domainPFAMPF13537GATase_7coord: 48..165
e-value: 4.6E-45
score: 152.5
IPR017932Glutamine amidotransferase type 2 domainPROSITEPS51278GATASE_TYPE_2coord: 2..185
score: 38.592232
NoneNo IPR availablePIRSRPIRSR001589-3PIRSR001589-3coord: 1..552
e-value: 3.3E-261
score: 865.3
NoneNo IPR availablePIRSRPIRSR000485-1PIRSR000485-1coord: 1..48
e-value: 0.73
score: 6.1
NoneNo IPR availablePIRSRPIRSR001589-1PIRSR001589-1coord: 1..552
e-value: 3.3E-261
score: 865.3
NoneNo IPR availablePIRSRPIRSR001589-2PIRSR001589-2coord: 1..552
e-value: 3.3E-261
score: 865.3
NoneNo IPR availablePANTHERPTHR11772:SF38ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 3coord: 1..557
NoneNo IPR availablePANTHERPTHR11772ASPARAGINE SYNTHETASEcoord: 1..557
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 209..484
IPR033738Asparagine synthase, N-terminal domainCDDcd00712AsnBcoord: 2..192
e-value: 6.7632E-86
score: 264.805

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g059320.m01Csor.00g059320.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006529 asparagine biosynthetic process
biological_process GO:0006541 glutamine metabolic process
cellular_component GO:0005829 cytosol
molecular_function GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity
molecular_function GO:0005524 ATP binding