Csor.00g053950 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g053950
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptiontRNA (Cytosine(38)-C(5))-methyltransferase
LocationCsor_Chr12: 1775421 .. 1777310 (-)
RNA-Seq ExpressionCsor.00g053950
SyntenyCsor.00g053950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGGCATTTCCAAAGCAGCAAGACGAACATTGGAGAGTCCTTGAATTCTTCAGCGGCATTGGTGGCATAGTACTCTTTTCGCCTATGCTTCTCTCTGTATCCTCCTCGATCTCAGGCGGATATCGTACAGAGGCTTTGTTTGCGATTTCAATTATAACAGAGATACTCTCTACTGAGTGCTGGCGTCGATGCGAAAGTAGTACGAGCTTTTGATATCAACGATAAAGCAAACGACGTTTACGAGCATAATTTCGGCGATCGTCCGCATCAGGTATGATAGAATCATTTTCTTCTTATATCGGTTTGAGAACGCCTTGCTTTCTGCCGTATGAATAAGTATTCGGTGCTTCTCAGTGAAAACATTGAAACTAGTTTTCCTTCAAAATCGAATGCAAATTCTCTTCAGTTGTGGTTTAAGTAGATTGAATTTGAGGCTGTTGAATGTTGTAGCCAAGCTTATTTAATTAGTGAGGAACTACCTCCTGGATACTGATGCACGTATGGTATGATAAGCTTTCAATTTACAGCTAGTATGAATTCACAATGTGAGCGGACAATAGATAGGTACATGAAGTGTTTTTGTTGGAGTATCTGATTCAAATGACTGCCGTTGTGGACTTTCTTTTGAACTGCCAAAGTAAAAGCGTTCTACAATCTCTCAACATCGTCTTCTTCTTTTATTCCTTGTTTCGAGAACGATTATGTTGCTTGTCCATCGCAGGAGTAAAGTTTTGCTGCTTCTCAATACAGATAACGGAAGTATTTTGTTCATTCAGAATTGAGATGCCAATTCTTTTCTATCGTGGATTAAGTAGATTGAATTTGAAGTTATTGAATGTTGAAACATAGCTTATCTATTTCGTTGGAAATTACCTACCTCTGACCGATGGACGTATTTGTTAAGTGATTAGATTACAATTTGAAGCTCGTATGAACTCTCAACGTAGTGGAGAACAAGGTAGCTATATGACGTATTTTTGTTGGAATCTCTTATTCAAATGATTAGCGTAGTGAATTTTTTCTTTCATCGATTCGTCAAACCAAAAGCGTCATGCTATTTAGGAACATGTAACAGGTCATTCATAGCTTCCTTTGCATAGGAGTGTCGGTTTTTTCTTATGAGTTTAGTTATGGTCAAAGCAATCCTACGTGCTTGGTCAAGCTACTGTAAAGACTCAGAGAGCAACAGAGAGCTACAAGAAAGAGGCAGAACTCGAGTCTAAAAAACGAATCATGGAGAGACCTTGAATTCTGCAGTGTTATTGATGGTATGATAGCTCGCATTTGAATGAGATTACCCAGTCTGGTTTCCTTAGTCCTTGGATCTGTCGTCTAATTTGGAGTCTGGACGTTTAGGATACTAAAGTAATTCTTAGATTTTGCATGCATAGCTTCCTATGGATAGGAGTGTCATCTTTTTCTTGTCGGTTAGTTATGGTCAAAAGTAACCTATATGCTTGGTTGAGCTACAGAAAGAAAAAGAGCGCTACAGGAAAGACTCAAAACTAGAGCCTAAAAAAAATGTACCATGGAGAGTCCTTGAATTCTTCAGCAACATCGGTTGCATGGTCGGTCTCATTTGAATTCTCATTTTTCCCAACTATATAGGTTTTCCATACTTTGTCCTTGGATCTGTTATCTAATTTGGAGTTTAGTCGTCTAGGATGCTGAAGAAATTCTTATATTTCATGTCCATATTGGTCCTCAGAGGTTAGTCACCTCATTTTGTGTATCGTTTTTGTTAGGACCGTACAACACATTTTATGCTTGTACATTGTGCATGTTATAAATTGGAAAGAAGATAATTATGCACCATATAATTAGGTCTACAAAAGCATTCCAGCGATGCTCGGGCATTTTCATTCCTCAAAATTCTTGAGTTGA

mRNA sequence

ATGGAGAAGGCATTTCCAAAGCAGCAAGACGAACATTGGAGAGTCCTTGAATTCTTCAGCGGCATTGGTGGCATAAGATACTCTCTACTGAGTGCTGGCGTCGATGCGAAAGTAGTACGAGCTTTTGATATCAACGATAAAGCAAACGACGTTTACGAGCATAATTTCGGCGATCGTCCGCATCAGGTCTACAAAAGCATTCCAGCGATGCTCGGGCATTTTCATTCCTCAAAATTCTTGAGTTGA

Coding sequence (CDS)

ATGGAGAAGGCATTTCCAAAGCAGCAAGACGAACATTGGAGAGTCCTTGAATTCTTCAGCGGCATTGGTGGCATAAGATACTCTCTACTGAGTGCTGGCGTCGATGCGAAAGTAGTACGAGCTTTTGATATCAACGATAAAGCAAACGACGTTTACGAGCATAATTTCGGCGATCGTCCGCATCAGGTCTACAAAAGCATTCCAGCGATGCTCGGGCATTTTCATTCCTCAAAATTCTTGAGTTGA

Protein sequence

MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRPHQVYKSIPAMLGHFHSSKFLS
Homology
[Close]
BLAST of Csor.00g053950 vs. ExPASy Swiss-Prot
Match: F4JWT7 (tRNA (cytosine(38)-C(5))-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=DNMT2 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 1.3e-14
Identity = 33/50 (66.00%), Postives = 45/50 (90.00%), Query Frame = 0 

Query: 13 WRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRPHQ 63
          W+VLEF+SGIGG+RYSL+++G+ ++VV AF+IND ANDVY+HNF  RP+Q
Sbjct: 15 WQVLEFYSGIGGMRYSLMASGIVSEVVEAFEINDSANDVYQHNFKHRPYQ 64

[Close]
BLAST of Csor.00g053950 vs. ExPASy Swiss-Prot
Match: Q54JH6 (DNA (cytosine-5)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=dnmA PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 8.8e-08
Identity = 26/52 (50.00%), Postives = 36/52 (69.23%), Query Frame = 0 

Query: 11 EHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRPHQ 63
          E  RVLEF+SGIGG+ Y L  +GVD +V+++FDIN  AN  Y++ F +   Q
Sbjct: 2  EQLRVLEFYSGIGGMHYGLQESGVDFQVIQSFDINTNANLNYKYTFNEDSSQ 53

[Close]
BLAST of Csor.00g053950 vs. ExPASy Swiss-Prot
Match: O55055 (tRNA (cytosine(38)-C(5))-methyltransferase OS=Mus musculus OX=10090 GN=Trdmt1 PE=1 SV=2)

HSP 1 Score: 53.9 bits (128), Expect = 9.7e-07
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 0 

Query: 11 EHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRPH 62
          E  RVLE +SGIGG+ ++L  + + A VV A D+N  AN+VY+HNF   PH
Sbjct: 2  EPLRVLELYSGIGGMHHALRESHIPAHVVAAIDVNTVANEVYKHNF---PH 49

[Close]
BLAST of Csor.00g053950 vs. ExPASy Swiss-Prot
Match: Q4G073 (tRNA (cytosine(38)-C(5))-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trdmt1 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.8e-06
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 0 

Query: 11 EHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRPH 62
          E  RVLE +SGIGG+ ++L  + V A VV A D++  AN+VY+HNF   PH
Sbjct: 2  EPLRVLELYSGIGGMHHALRESRVPAHVVAAIDVSTVANEVYKHNF---PH 49

[Close]
BLAST of Csor.00g053950 vs. ExPASy Swiss-Prot
Match: O14717 (tRNA (cytosine(38)-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=TRDMT1 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 4.8e-06
Identity = 24/51 (47.06%), Postives = 36/51 (70.59%), Query Frame = 0 

Query: 11 EHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRPH 62
          E  RVLE +SG+GG+ ++L  + + A+VV A D+N  AN+VY++NF   PH
Sbjct: 2  EPLRVLELYSGVGGMHHALRESCIPAQVVAAIDVNTVANEVYKYNF---PH 49

[Close]
BLAST of Csor.00g053950 vs. NCBI nr
Match: KAG6585458.1 (tRNA (cytosine(38)-C(5))-methyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 171 bits (434), Expect = 7.11e-54
Identity = 81/81 (100.00%), Postives = 81/81 (100.00%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60

Query: 61 HQVYKSIPAMLGHFHSSKFLS 81
          HQVYKSIPAMLGHFHSSKFLS
Sbjct: 61 HQVYKSIPAMLGHFHSSKFLS 81

[Close]
BLAST of Csor.00g053950 vs. NCBI nr
Match: KAG7020373.1 (DNA (cytosine-5)-methyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 137 bits (345), Expect = 4.93e-40
Identity = 64/72 (88.89%), Postives = 67/72 (93.06%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60

Query: 61 HQVYKSIPAMLG 72
          HQ++     +LG
Sbjct: 61 HQLWSKQSYVLG 72

[Close]
BLAST of Csor.00g053950 vs. NCBI nr
Match: XP_008445162.1 (PREDICTED: tRNA (cytosine(38)-C(5))-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 115 bits (289), Expect = 1.16e-28
Identity = 53/62 (85.48%), Postives = 58/62 (93.55%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK FPK QDE WRVLEF+SGIGG+RYSLLSAG+ AKVV+AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 62
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Csor.00g053950 vs. NCBI nr
Match: XP_004138735.1 (tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 115 bits (289), Expect = 1.16e-28
Identity = 53/62 (85.48%), Postives = 58/62 (93.55%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK FPK QDE WRVLEF+SGIGG+RYSLLSAG+ AKVV+AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 62
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Csor.00g053950 vs. NCBI nr
Match: KAE8652193.1 (hypothetical protein Csa_022276 [Cucumis sativus])

HSP 1 Score: 115 bits (289), Expect = 1.23e-28
Identity = 53/62 (85.48%), Postives = 58/62 (93.55%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK FPK QDE WRVLEF+SGIGG+RYSLLSAG+ AKVV+AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 62
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Csor.00g053950 vs. ExPASy TrEMBL
Match: A0A0A0LSE8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G380010 PE=3 SV=1)

HSP 1 Score: 115 bits (289), Expect = 5.62e-29
Identity = 53/62 (85.48%), Postives = 58/62 (93.55%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK FPK QDE WRVLEF+SGIGG+RYSLLSAG+ AKVV+AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 62
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Csor.00g053950 vs. ExPASy TrEMBL
Match: A0A1S3BC07 (tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488282 PE=3 SV=1)

HSP 1 Score: 115 bits (289), Expect = 5.62e-29
Identity = 53/62 (85.48%), Postives = 58/62 (93.55%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK FPK QDE WRVLEF+SGIGG+RYSLLSAG+ AKVV+AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 62
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Csor.00g053950 vs. ExPASy TrEMBL
Match: A0A5A7VCE7 (tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002740 PE=3 SV=1)

HSP 1 Score: 115 bits (289), Expect = 7.46e-29
Identity = 53/62 (85.48%), Postives = 58/62 (93.55%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK FPK QDE WRVLEF+SGIGG+RYSLLSAG+ AKVV+AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 62
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Csor.00g053950 vs. ExPASy TrEMBL
Match: A0A6J1BVV7 (DNA (Cytosine-5)-methyltransferase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005184 PE=3 SV=1)

HSP 1 Score: 111 bits (278), Expect = 2.19e-27
Identity = 52/62 (83.87%), Postives = 57/62 (91.94%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK  P+ Q E WRVLEF+SGIGG+RYSLLSAGVDAKVV+AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 62
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Csor.00g053950 vs. ExPASy TrEMBL
Match: A0A6J1HD73 (tRNA (Cytosine(38)-C(5))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111462432 PE=3 SV=1)

HSP 1 Score: 110 bits (275), Expect = 6.12e-27
Identity = 50/62 (80.65%), Postives = 56/62 (90.32%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK  PK Q E WRVLEF+SGIGG+RYSLL AG+DAKV++AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVSPKLQGEPWRVLEFYSGIGGMRYSLLRAGIDAKVIQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 62
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Csor.00g053950 vs. TAIR 10
Match: AT5G25480.1 (DNA methyltransferase-2 )

HSP 1 Score: 80.1 bits (196), Expect = 9.0e-16
Identity = 33/50 (66.00%), Postives = 45/50 (90.00%), Query Frame = 0 

Query: 13 WRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRPHQ 63
          W+VLEF+SGIGG+RYSL+++G+ ++VV AF+IND ANDVY+HNF  RP+Q
Sbjct: 15 WQVLEFYSGIGGMRYSLMASGIVSEVVEAFEINDSANDVYQHNFKHRPYQ 64

The following BLAST results are available for this feature:
BLAST of Csor.00g053950 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4JWT71.3e-1466.00tRNA (cytosine(38)-C(5))-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q54JH68.8e-0850.00DNA (cytosine-5)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=dnmA ... [more]
O550559.7e-0750.98tRNA (cytosine(38)-C(5))-methyltransferase OS=Mus musculus OX=10090 GN=Trdmt1 PE... [more]
Q4G0732.8e-0650.98tRNA (cytosine(38)-C(5))-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trdm... [more]
O147174.8e-0647.06tRNA (cytosine(38)-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=TRDMT1 PE=... [more]
BLAST of Csor.00g053950 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6585458.17.11e-54100.00tRNA (cytosine(38)-C(5))-methyltransferase 2, partial [Cucurbita argyrosperma su... [more]
KAG7020373.14.93e-4088.89DNA (cytosine-5)-methyltransferase, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_008445162.11.16e-2885.48PREDICTED: tRNA (cytosine(38)-C(5))-methyltransferase isoform X1 [Cucumis melo][more]
XP_004138735.11.16e-2885.48tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X2 [Cucumis sativus][more]
KAE8652193.11.23e-2885.48hypothetical protein Csa_022276 [Cucumis sativus][more]
BLAST of Csor.00g053950 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LSE85.62e-2985.48Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G380010 PE=3 SV=1[more]
A0A1S3BC075.62e-2985.48tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN... [more]
A0A5A7VCE77.46e-2985.48tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo var. makuw... [more]
A0A6J1BVV72.19e-2783.87DNA (Cytosine-5)-methyltransferase isoform X1 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1HD736.12e-2780.65tRNA (Cytosine(38)-C(5))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
BLAST of Csor.00g053950 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G25480.19.0e-1666.00DNA methyltransferase-2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001525C-5 cytosine methyltransferasePFAMPF00145DNA_methylasecoord: 14..60
e-value: 5.6E-7
score: 29.2
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 15..62
e-value: 5.6E-16
score: 60.6
NoneNo IPR availablePANTHERPTHR46098TRNA (CYTOSINE(38)-C(5))-METHYLTRANSFERASEcoord: 10..62
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 13..62

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g053950.m01Csor.00g053950.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity