Csor.00g049000 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g049000
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionNon-structural maintenance of chromosomes element 4
LocationCsor_Chr11: 4257155 .. 4259464 (-)
RNA-Seq ExpressionCsor.00g049000
SyntenyCsor.00g049000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCCTGTCAAACGGGAACCCGCCGCCAGTAGCCGCAGTAATCGGAGTAAAGGCGGCGACGCCGTCGATGCTGCGAATTTAAGAGATGTTAAGCGGGCGAGGGTTGCTCGGGAGAAAGAGAACATCGGTGAGGGCAACATGGCTGAAGCTGAAGAGGGTCAGCAAGGCTTGACGAAGCGGCGGGTTCTCCGGTCTCGGTATCTTGCGGTCATTCACGAGATTAGTGGTATGGTGGATCTCTGCATTTTTCTTTTCTTTTTTTTAATTACTACTGGATCTCTGCATTAGTGTGTGTAGGTAATCGGGTTTTCTTTTTTTGGTTTCTTGTTTTTTGAAGAAAGGAGAGAGGATTTGACGAAGGATTCGGACAAGTTCAATGTTATCATCAATGAAGTGGAAAAGTTGCACGAGCAAGGTATTTCTGTAGTTTATTTTCGATTTGTTTACGTTGAAGAACTGTTGTATCTTTGATTTAAAATTTTCATCAAAAGAAAACAATCAAACATTGCCACTTTTCCGTTGTTGCATTTTCCCCTTGCACTCTCATAGCAGTTTGAATGTGACTTGAACTTCGAGTTTTGGAAGGGGGCTTGTTCCTTTTCCTTTTGATATTGTTACTCATGTTATATGGTTGTGTTTCCATCTGGGTTTCAGTTCAGAAGCCAAGGGAACAAGTTGCAGATGCAGAAGCCTTGCAAGATATTGCAAACTCCCTCGTGACTTCTATTAAGTCGCAGTCAAATGAAGGTGTTACTCCATCAGATTTTGTGAGCTGTCTGCTCAAAGAATTTGCTAACATTGAAGGCGGTATAACATCCGAAGAAAACGATCCGATTTCTGTCAGTTGGAAAGATATTGGATTAAGCGTTTCGTCTATTTTCATGAATGGCCATGGATGCTGCACCATGTGAGACTCTTAAATTCCTTTTTCTCTCGAACTTAATGCTAAAGTGGCTTCTATGGATGTTTTTACCAAGTATATAACTTCTTTCTTGTGTCTTTTTCAAGGCTTGGACCTATGAGCACTCAGTTGAAGCAGCGAAAGACTAATATTGCCCGGAAACGTTCAAGGCCTACTGAAAGTTCAAGACCTGCAGAGGTTAGACTTTAACTTCAATACTACAGTCAAATTTTTAAGCATCTTGGAGATTAGTGTAAACATGTTTTAGATTATTTTATTAGATGGTGAGTGTCCAATTTTACTGGAAGTACAGATATTTCTTTTCGGGTAGGACATATATAATTGAACTCATTAACGTTTTAGCCGAACCTTACGAAGCTTGTTTGTAGTCACACCTCTAGCTTTCCTGCTTGATGTTCTTTGCAGATACTTATTAACTTTTTTCTGTTTGGTCAAATGGTTTGCAAAATAGGTTTCATTTATGTTTGTTTTTCATGCATTAGATATTCTTTTGTCTCGTTGATTTTTATCTCAATTCATATGTGCAGGTTGATGAAGATGGATCTGAAGAAAAAACGGATACAGATAAGAACATGAGCATCATGTTTGGAATTTTGAGGAGGAATAAAACCGTTAGACTTGAACATTTGATATTGAATAGAAACTCTTTTGCACAGACAGTAGAGAACTTATTTGCTCTCTCCTTTCTGGTCAAGGATGGTCGGGCCGAGATAACTATTGATAAAAATGGTTCTCATTTTGTATGTGAGTAAACTTTTTGTACTTTTCTTTTAAAACATTGTGTTCTTTGCATTATACTTACATTTTCTGTCAATATTTTCAGCTCCGAAGAATGCCCCGGCCCATAGTGCCATTATGTCACACGAAGTTAATTATAGTCACTTCGTATTCAGATTCGATTTTAAGGATTGGAAGGTACAGAGAAGCATTCATTGCTATGATACTAACTTGACATTTCCCAGTGTCATGAAACAGAGTTCAAATATTAAGAAAAGTATTTTGCAGCTGTGTACTATGATTAGACTTGTATATAATATTGGATAGAGGCTCATAATATATATCAAATGCTGTGTATTATTTAGATCCTTGGCTTCAATTGAGGAACACATACTTGTGAAATGTATTTGCAGTTGATGATGGATATAGTACCAGTTGGAGAGGAGTTGATGCCCCACAGGAACTTTCTAAATTCAGCACCCATGCCCCAAGAAGAAGCAACCACGGACGATTCTGAATCAGCGTCATTGCCTACCACGCCAATTCGAAAGCTGTCAAGAAATCGAGGTTTAGTTATGCAAACTGTTGTTGAGGATTCCCCTGATAATGACGCTCATTCAGGACCAACTGCAATGTTGAGGTGCAAGAAAAAGCTTAGTCAAGGTTAG

mRNA sequence

ATGAAGCCTGTCAAACGGGAACCCGCCGCCAGTAGCCGCAGTAATCGGAGTAAAGGCGGCGACGCCGTCGATGCTGCGAATTTAAGAGATGTTAAGCGGGCGAGGGTTGCTCGGGAGAAAGAGAACATCGGTGAGGGCAACATGGCTGAAGCTGAAGAGGGTCAGCAAGGCTTGACGAAGCGGCGGGTTCTCCGGTCTCGGTATCTTGCGGTCATTCACGAGATTAGTGAAAGGAGAGAGGATTTGACGAAGGATTCGGACAAGTTCAATGTTATCATCAATGAAGTGGAAAAGTTGCACGAGCAAGTTCAGAAGCCAAGGGAACAAGTTGCAGATGCAGAAGCCTTGCAAGATATTGCAAACTCCCTCGTGACTTCTATTAAGTCGCAGTCAAATGAAGGTGTTACTCCATCAGATTTTGTGAGCTGTCTGCTCAAAGAATTTGCTAACATTGAAGGCGGTATAACATCCGAAGAAAACGATCCGATTTCTGTCAGTTGGAAAGATATTGGATTAAGCGTTTCGTCTATTTTCATGAATGGCCATGGATGCTGCACCATGCTTGGACCTATGAGCACTCAGTTGAAGCAGCGAAAGACTAATATTGCCCGGAAACGTTCAAGGCCTACTGAAAGTTCAAGACCTGCAGAGGTTGATGAAGATGGATCTGAAGAAAAAACGGATACAGATAAGAACATGAGCATCATGTTTGGAATTTTGAGGAGGAATAAAACCGTTAGACTTGAACATTTGATATTGAATAGAAACTCTTTTGCACAGACAGTAGAGAACTTATTTGCTCTCTCCTTTCTGGTCAAGGATGGTCGGGCCGAGATAACTATTGATAAAAATGGTTCTCATTTTGTATCTCCGAAGAATGCCCCGGCCCATAGTGCCATTATGTCACACGAAGTTAATTATAGTCACTTCGTATTCAGATTCGATTTTAAGGATTGGAAGTTGATGATGGATATAGTACCAGTTGGAGAGGAGTTGATGCCCCACAGGAACTTTCTAAATTCAGCACCCATGCCCCAAGAAGAAGCAACCACGGACGATTCTGAATCAGCGTCATTGCCTACCACGCCAATTCGAAAGCTGTCAAGAAATCGAGGTTTAGTTATGCAAACTGTTGTTGAGGATTCCCCTGATAATGACGCTCATTCAGGACCAACTGCAATGTTGAGGTGCAAGAAAAAGCTTAGTCAAGGTTAG

Coding sequence (CDS)

ATGAAGCCTGTCAAACGGGAACCCGCCGCCAGTAGCCGCAGTAATCGGAGTAAAGGCGGCGACGCCGTCGATGCTGCGAATTTAAGAGATGTTAAGCGGGCGAGGGTTGCTCGGGAGAAAGAGAACATCGGTGAGGGCAACATGGCTGAAGCTGAAGAGGGTCAGCAAGGCTTGACGAAGCGGCGGGTTCTCCGGTCTCGGTATCTTGCGGTCATTCACGAGATTAGTGAAAGGAGAGAGGATTTGACGAAGGATTCGGACAAGTTCAATGTTATCATCAATGAAGTGGAAAAGTTGCACGAGCAAGTTCAGAAGCCAAGGGAACAAGTTGCAGATGCAGAAGCCTTGCAAGATATTGCAAACTCCCTCGTGACTTCTATTAAGTCGCAGTCAAATGAAGGTGTTACTCCATCAGATTTTGTGAGCTGTCTGCTCAAAGAATTTGCTAACATTGAAGGCGGTATAACATCCGAAGAAAACGATCCGATTTCTGTCAGTTGGAAAGATATTGGATTAAGCGTTTCGTCTATTTTCATGAATGGCCATGGATGCTGCACCATGCTTGGACCTATGAGCACTCAGTTGAAGCAGCGAAAGACTAATATTGCCCGGAAACGTTCAAGGCCTACTGAAAGTTCAAGACCTGCAGAGGTTGATGAAGATGGATCTGAAGAAAAAACGGATACAGATAAGAACATGAGCATCATGTTTGGAATTTTGAGGAGGAATAAAACCGTTAGACTTGAACATTTGATATTGAATAGAAACTCTTTTGCACAGACAGTAGAGAACTTATTTGCTCTCTCCTTTCTGGTCAAGGATGGTCGGGCCGAGATAACTATTGATAAAAATGGTTCTCATTTTGTATCTCCGAAGAATGCCCCGGCCCATAGTGCCATTATGTCACACGAAGTTAATTATAGTCACTTCGTATTCAGATTCGATTTTAAGGATTGGAAGTTGATGATGGATATAGTACCAGTTGGAGAGGAGTTGATGCCCCACAGGAACTTTCTAAATTCAGCACCCATGCCCCAAGAAGAAGCAACCACGGACGATTCTGAATCAGCGTCATTGCCTACCACGCCAATTCGAAAGCTGTCAAGAAATCGAGGTTTAGTTATGCAAACTGTTGTTGAGGATTCCCCTGATAATGACGCTCATTCAGGACCAACTGCAATGTTGAGGTGCAAGAAAAAGCTTAGTCAAGGTTAG

Protein sequence

MKPVKREPAASSRSNRSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTKRRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFMNGHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGILRRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLPTTPIRKLSRNRGLVMQTVVEDSPDNDAHSGPTAMLRCKKKLSQG
Homology
BLAST of Csor.00g049000 vs. ExPASy Swiss-Prot
Match: Q9C689 (Non-structural maintenance of chromosomes element 4 homolog A OS=Arabidopsis thaliana OX=3702 GN=NSE4A PE=2 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 7.8e-105
Identity = 215/406 (52.96%), Postives = 284/406 (69.95%), Query Frame = 0

Query: 2   KPVKREPAASSRSNRSKGGDAVDAANLRDVKRARVAR-EKENIGEGNMAEAEEGQQGLTK 61
           K VKRE  A+     +      +   LR VK+ +  + E +++        +E +QG++ 
Sbjct: 3   KTVKRESEATGGKREADD----EPEKLRSVKKEKQRKTEADSVRPDEPPPPQEEEQGISD 62

Query: 62  RRVLRSRYLAVIHEISERREDLTK-DSDKFNVIINEVEKLHEQVQKPREQVADAEALQDI 121
           RR+LRS+YL++ +EI++ ++DL K DSDKF+ IIN VE LH+QV+KPREQ+ADAEAL DI
Sbjct: 63  RRILRSKYLSLQNEINDCKDDLMKIDSDKFSRIINAVENLHQQVRKPREQIADAEALLDI 122

Query: 122 ANSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFM 181
           AN+L++S+KSQS  GV+P++FV+ L+  F     GI ++E   +S+ WKD+G +V S  +
Sbjct: 123 ANTLMSSVKSQSAHGVSPAEFVNALISGFGQGSLGIDTDETAQVSLKWKDLGFAVCSTVL 182

Query: 182 NGHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGI 241
              GC TMLGPM T+LKQRK    RKR++P E  RP EVD+  SEEKTDTDKNM+IMF I
Sbjct: 183 VSCGCSTMLGPMDTELKQRKRAPNRKRTKPGEGVRPDEVDDSQSEEKTDTDKNMTIMFNI 242

Query: 242 LRRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSA 301
           L + K V+LE+L+LNR SFAQTVENLFALSFL KDGR EI +DK+GSHF  P+NAP  + 
Sbjct: 243 LGKKKRVQLENLVLNRRSFAQTVENLFALSFLAKDGRVEIIVDKSGSHFAMPRNAPDANV 302

Query: 302 IMSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHR-NFLNSAPMPQEEATTDDSESAS 361
           +MS EV Y+HFVFR DFKDWKLM ++VP+GEELMPHR   + S+  P   A      +  
Sbjct: 303 VMSGEVIYNHFVFRLDFKDWKLMSEMVPLGEELMPHRQTAVASSSCPAASAPASADFTQD 362

Query: 362 LPTTPIRKLSRNRGLVMQ--TVVEDSPDNDAHSGPTAMLRCKKKLS 403
             TTPIRKLSRNRGLV+Q  TVVED+PD +   G     RCK++L+
Sbjct: 363 TQTTPIRKLSRNRGLVVQEETVVEDTPDKE---GDGTRRRCKRRLT 401

BLAST of Csor.00g049000 vs. ExPASy Swiss-Prot
Match: F4JET1 (Non-structural maintenance of chromosomes element 4 homolog B OS=Arabidopsis thaliana OX=3702 GN=NSE4B PE=2 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 3.5e-89
Identity = 178/336 (52.98%), Postives = 242/336 (72.02%), Query Frame = 0

Query: 52  EEGQQGLTKRRVLRSRYLAVIHEISERREDLTK-DSDKFNVIINEVEKLHEQVQKPREQV 111
           EE +QG+  RR +RS+YLA+ H+I + ++DLTK DS+KFN IINEVE LH++V+KPREQ+
Sbjct: 53  EEEEQGIADRRAIRSQYLALTHKIKDAKDDLTKIDSNKFNSIINEVENLHQKVRKPREQI 112

Query: 112 ADAEALQDIANSLVTSIKSQS-NEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKD 171
           ADAEAL D+ NS+V+S+KSQS + GV+P++FV+ L+  F      I ++EN  +S+ WKD
Sbjct: 113 ADAEALLDLTNSVVSSVKSQSAHGGVSPAEFVNALINGFGKTSLRIDADENTQVSMKWKD 172

Query: 172 IGLSVSSIFMNGHGCCTMLGPMSTQLKQRKTNIA-RKRSRPTESSRPAEVDEDGSEEKTD 231
           +G +V S  +   GC TM+GPM +++KQRK+ +  RKR++P    +P EVD+  +E+K+D
Sbjct: 173 LGFTVCSTVLVSCGCTTMMGPMYSEMKQRKSRVGNRKRTKPGAGVKPEEVDDTEAEKKSD 232

Query: 232 TDKNMSIMFGILRRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHF 291
           TD NM++MF ILR+NK V++E+L+LNR SFAQT EN+FALSFLVKDGR EIT+D NGSHF
Sbjct: 233 TDNNMAVMFNILRKNKRVKIENLVLNRKSFAQTAENMFALSFLVKDGRVEITVDNNGSHF 292

Query: 292 VSPKNAPAHSAIMSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEE 351
           V P+NAPA + ++S EV Y+HFV RFD+KDW+ M  +V VGEELMP R          E 
Sbjct: 293 VEPRNAPAANLVLSGEVAYNHFVLRFDYKDWEPMSKMVAVGEELMPLR----------ET 352

Query: 352 ATTDDSESASLPTTPIRKLSRNRGLVMQ--TVVEDS 383
               DS           +LSR +G V+Q  TVVEDS
Sbjct: 353 KVAQDS----------IELSRKQGSVIQEETVVEDS 368

BLAST of Csor.00g049000 vs. ExPASy Swiss-Prot
Match: Q2TBI1 (Non-structural maintenance of chromosomes element 4 homolog A OS=Bos taurus OX=9913 GN=NSMCE4A PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.2e-12
Identity = 68/292 (23.29%), Postives = 127/292 (43.49%), Query Frame = 0

Query: 62  RVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEAL---QD 121
           R +R +Y A+I+ + + RED+   SDK   ++ E   L   V + RE V DA  L    D
Sbjct: 83  RQIRHQYRALINSVQQNREDILNASDKLTEVLEEANTLFNGVSRAREAVLDAHFLVLASD 142

Query: 122 IANSLVTSIKSQSNEGVTPSDFVSCLLKEFA----NIEGGITSEENDPISV----SWKDI 181
           +       ++S  N       +V  LL          E  I  E++  +      SWK  
Sbjct: 143 LGKEKAKQLRSDLN-SFDMLRYVETLLTHMGVNPLEAEELIRDEDSSDLEFIVYDSWKIS 202

Query: 182 GLSVSSIFMNGHGCCTMLGPMSTQLKQRKTNIARKRS---RPTESSRPAEVDEDGSEEKT 241
           G +  + F   H    +LG +  +    K  I R R       + + PA++       + 
Sbjct: 203 GKTAENTFNKTHTFHFLLGSIQGECPVPKPRIERPRKVRMIEEQQAMPAQLKRMEESHQE 262

Query: 242 DTDKNMSIMFGILRR------NKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITI 301
            T+K +  + G+L+       +  +     +++ +SF +TVEN+F +SF+++DG A I +
Sbjct: 263 ATEKEVERILGLLQTYFQEDPDTPMSFFDFVVDPHSFPRTVENIFHVSFIIRDGFARIRL 322

Query: 302 DKNGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELM 334
           D++    + P N    S   +   N +  +    ++DW+ ++    + E ++
Sbjct: 323 DRDRLPVIEPVNINEESGGNTQIRNQA--IIALSYRDWEEIVRTFEISEPVI 371

BLAST of Csor.00g049000 vs. ExPASy Swiss-Prot
Match: Q9NXX6 (Non-structural maintenance of chromosomes element 4 homolog A OS=Homo sapiens OX=9606 GN=NSMCE4A PE=1 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 3.5e-12
Identity = 78/344 (22.67%), Postives = 147/344 (42.73%), Query Frame = 0

Query: 11  SSRSNRSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTKRRVLRSRYLA 70
           S RS R    +  +A     ++    +   + + +    EA E  QGL   R +R +Y A
Sbjct: 35  SPRSRRGSARERREAPERPSLEDTEPSDSGDEMMDPASLEA-EADQGLC--RQIRHQYRA 94

Query: 71  VIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIKSQ 130
           +I+ + + RED+    DK   ++ E   L  +V + RE V DA  L  +A+ L      Q
Sbjct: 95  LINSVQQNREDILNAGDKLTEVLEEANTLFNEVSRAREAVLDAHFLV-LASDLGKEKAKQ 154

Query: 131 SNEGVTPSD---FVSCLLKEFA----NIEGGITSEENDPISV----SWKDIGLSVSSIFM 190
               ++  D   +V  LL          E  I  E++         SWK  G +  + F 
Sbjct: 155 LRSDLSSFDMLRYVETLLTHMGVNPLEAEELIRDEDSPDFEFIVYDSWKITGRTAENTFN 214

Query: 191 NGHGCCTMLGPMSTQLKQRKTNIARKRSRPT---ESSRPAEVDEDGSEEKTDTDKNMSIM 250
             H    +LG +  +    K  + R R  P    E + PA++       +  T+K +  +
Sbjct: 215 KTHTFHFLLGSIYGECPVPKPRVDRPRKVPVIQEERAMPAQLRRMEESHQEATEKEVERI 274

Query: 251 FGILRR------NKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVS 310
            G+L+       +  +     +++ +SF +TVEN+F +SF+++DG A I +D++    + 
Sbjct: 275 LGLLQTYFREDPDTPMSFFDFVVDPHSFPRTVENIFHVSFIIRDGFARIRLDQDRLPVIE 334

Query: 311 PKNAPAHSAIMSHEVNY-SHFVFRFDFKDWKLMMDIVPVGEELM 334
           P +    +    H     +  +    ++DW+ ++    + E ++
Sbjct: 335 PVSINEENEGFEHNTQVRNQGIIALSYRDWEEIVKTFEISEPVI 374

BLAST of Csor.00g049000 vs. ExPASy Swiss-Prot
Match: Q6BDR8 (Non-structural maintenance of chromosome element 4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=nse4 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.0e-11
Identity = 69/285 (24.21%), Postives = 128/285 (44.91%), Query Frame = 0

Query: 61  RRVLRSRYLAVIHEISERREDLT-KDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDI 120
           +R LR RY  +I+++ E R +L  ++++     I     L   V  P E   DA  L   
Sbjct: 6   KRDLRKRYRNLINKVQESRLELVDEENNNLYETITTANDLFSSVDAPTEATLDALLLTKT 65

Query: 121 ANSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEEN----DPISVSWKDIGLSVS 180
            +  + SIK++      P   +    K         TS  N      I  +W  +G   S
Sbjct: 66  VD--LASIKARQLHIGRPKFNIELFTKNIKQFLNYPTSHSNVTRIQEIDTAWSRLGKLAS 125

Query: 181 SIFMNGHGCCTMLGPMSTQLKQRKTNIARK---RSRPTESSRPAEVDEDG-SEEKTDTDK 240
           +          M+GP+S + K+R  NI R+   +  P   ++P  ++E   + ++ +T K
Sbjct: 126 NCEKQPASLNLMVGPLSFRKKER--NIQRRERLQKAPNVLTQPTMLNERNITTQENNTTK 185

Query: 241 NMSIMFGILRRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSP 300
           N+  +  +L+ ++ V     I N  S+ QTVENLF +SFL K+G+A +  +++G   +  
Sbjct: 186 NVLHISRLLQAHQPVNFLKFITNPQSYPQTVENLFYVSFLFKEGKAALVENESGILMLET 245

Query: 301 KNAPAHSAIMSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHR 337
           +  P    +++ E+     V       ++ ++    + E ++P R
Sbjct: 246 RIPPTDDQVVAGEIRNIQLVLDMTMDLYENIIKEYNIKESIIPTR 286

BLAST of Csor.00g049000 vs. NCBI nr
Match: KAG6588168.1 (Non-structural maintenance of chromosomes element 4-like A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 781 bits (2017), Expect = 1.64e-284
Identity = 404/404 (100.00%), Postives = 404/404 (100.00%), Query Frame = 0

Query: 1   MKPVKREPAASSRSNRSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60
           MKPVKREPAASSRSNRSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK
Sbjct: 1   MKPVKREPAASSRSNRSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFMN 180
           NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240
           GHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL
Sbjct: 181 GHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360
           MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360

Query: 361 TTPIRKLSRNRGLVMQTVVEDSPDNDAHSGPTAMLRCKKKLSQG 404
           TTPIRKLSRNRGLVMQTVVEDSPDNDAHSGPTAMLRCKKKLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQTVVEDSPDNDAHSGPTAMLRCKKKLSQG 404

BLAST of Csor.00g049000 vs. NCBI nr
Match: KAG7022067.1 (Non-structural maintenance of chromosomes element 4-like A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 780 bits (2013), Expect = 6.66e-284
Identity = 403/404 (99.75%), Postives = 404/404 (100.00%), Query Frame = 0

Query: 1   MKPVKREPAASSRSNRSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60
           MKPVKREPAASSRSN+SKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK
Sbjct: 1   MKPVKREPAASSRSNQSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFMN 180
           NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240
           GHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL
Sbjct: 181 GHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360
           MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360

Query: 361 TTPIRKLSRNRGLVMQTVVEDSPDNDAHSGPTAMLRCKKKLSQG 404
           TTPIRKLSRNRGLVMQTVVEDSPDNDAHSGPTAMLRCKKKLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQTVVEDSPDNDAHSGPTAMLRCKKKLSQG 404

BLAST of Csor.00g049000 vs. NCBI nr
Match: XP_022933648.1 (non-structural maintenance of chromosomes element 4 homolog A-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 764 bits (1972), Expect = 1.18e-277
Identity = 396/404 (98.02%), Postives = 401/404 (99.26%), Query Frame = 0

Query: 1   MKPVKREPAASSRSNRSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60
           M+PVKREPAASSRSN+SKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK
Sbjct: 1   MRPVKREPAASSRSNQSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFMN 180
           NSLVTSIKSQSNEGVTPSDFVSCLLKEFA+IEGGITSEEN+PIS+SWKDIGLSVSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFADIEGGITSEENNPISLSWKDIGLSVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240
           GHGC TMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSE KTDTDKNMSIMFGIL
Sbjct: 181 GHGCRTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEAKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360
           MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360

Query: 361 TTPIRKLSRNRGLVMQTVVEDSPDNDAHSGPTAMLRCKKKLSQG 404
           TTPIRKLSRNRGLVMQTVVEDSPDN AHSGPTAMLRCKKKLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQTVVEDSPDNGAHSGPTAMLRCKKKLSQG 404

BLAST of Csor.00g049000 vs. NCBI nr
Match: XP_022933647.1 (non-structural maintenance of chromosomes element 4 homolog A-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 764 bits (1972), Expect = 1.37e-277
Identity = 396/404 (98.02%), Postives = 401/404 (99.26%), Query Frame = 0

Query: 1   MKPVKREPAASSRSNRSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60
           M+PVKREPAASSRSN+SKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK
Sbjct: 1   MRPVKREPAASSRSNQSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFMN 180
           NSLVTSIKSQSNEGVTPSDFVSCLLKEFA+IEGGITSEEN+PIS+SWKDIGLSVSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFADIEGGITSEENNPISLSWKDIGLSVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240
           GHGC TMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSE KTDTDKNMSIMFGIL
Sbjct: 181 GHGCRTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEAKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360
           MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360

Query: 361 TTPIRKLSRNRGLVMQTVVEDSPDNDAHSGPTAMLRCKKKLSQG 404
           TTPIRKLSRNRGLVMQTVVEDSPDN AHSGPTAMLRCKKKLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQTVVEDSPDNGAHSGPTAMLRCKKKLSQG 404

BLAST of Csor.00g049000 vs. NCBI nr
Match: XP_023530878.1 (non-structural maintenance of chromosomes element 4 homolog A-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 758 bits (1958), Expect = 1.67e-275
Identity = 393/405 (97.04%), Postives = 399/405 (98.52%), Query Frame = 0

Query: 1   MKPVKREPAASSRSNRSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60
           M+PVKREPAASSRSN+SKGGDAVDA NLRDVKRARV+REKENIGEGNMAEAEEGQQGLTK
Sbjct: 1   MRPVKREPAASSRSNQSKGGDAVDAVNLRDVKRARVSREKENIGEGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFMN 180
           NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGL VSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLGVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240
           GHGC TMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL
Sbjct: 181 GHGCRTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKT RLEHLILNRNSFAQT+ENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTARLEHLILNRNSFAQTIENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360
           MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAP+PQEEA TDDSESA+LP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPIPQEEAATDDSESATLP 360

Query: 361 TTPIRKLSRNRGLVMQTVVEDSPDND-AHSGPTAMLRCKKKLSQG 404
           TTPIRKLSRNRGLVMQTVVEDSPDND AHSGPTAMLRCKKKLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQTVVEDSPDNDGAHSGPTAMLRCKKKLSQG 405

BLAST of Csor.00g049000 vs. ExPASy TrEMBL
Match: A0A6J1F5E6 (Non-structural maintenance of chromosomes element 4 OS=Cucurbita moschata OX=3662 GN=LOC111441004 PE=3 SV=1)

HSP 1 Score: 764 bits (1972), Expect = 5.71e-278
Identity = 396/404 (98.02%), Postives = 401/404 (99.26%), Query Frame = 0

Query: 1   MKPVKREPAASSRSNRSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60
           M+PVKREPAASSRSN+SKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK
Sbjct: 1   MRPVKREPAASSRSNQSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFMN 180
           NSLVTSIKSQSNEGVTPSDFVSCLLKEFA+IEGGITSEEN+PIS+SWKDIGLSVSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFADIEGGITSEENNPISLSWKDIGLSVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240
           GHGC TMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSE KTDTDKNMSIMFGIL
Sbjct: 181 GHGCRTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEAKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360
           MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360

Query: 361 TTPIRKLSRNRGLVMQTVVEDSPDNDAHSGPTAMLRCKKKLSQG 404
           TTPIRKLSRNRGLVMQTVVEDSPDN AHSGPTAMLRCKKKLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQTVVEDSPDNGAHSGPTAMLRCKKKLSQG 404

BLAST of Csor.00g049000 vs. ExPASy TrEMBL
Match: A0A6J1F0F0 (Non-structural maintenance of chromosomes element 4 OS=Cucurbita moschata OX=3662 GN=LOC111441004 PE=3 SV=1)

HSP 1 Score: 764 bits (1972), Expect = 6.64e-278
Identity = 396/404 (98.02%), Postives = 401/404 (99.26%), Query Frame = 0

Query: 1   MKPVKREPAASSRSNRSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60
           M+PVKREPAASSRSN+SKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK
Sbjct: 1   MRPVKREPAASSRSNQSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFMN 180
           NSLVTSIKSQSNEGVTPSDFVSCLLKEFA+IEGGITSEEN+PIS+SWKDIGLSVSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFADIEGGITSEENNPISLSWKDIGLSVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240
           GHGC TMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSE KTDTDKNMSIMFGIL
Sbjct: 181 GHGCRTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEAKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360
           MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360

Query: 361 TTPIRKLSRNRGLVMQTVVEDSPDNDAHSGPTAMLRCKKKLSQG 404
           TTPIRKLSRNRGLVMQTVVEDSPDN AHSGPTAMLRCKKKLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQTVVEDSPDNGAHSGPTAMLRCKKKLSQG 404

BLAST of Csor.00g049000 vs. ExPASy TrEMBL
Match: A0A6J1HSJ2 (Non-structural maintenance of chromosomes element 4 OS=Cucurbita maxima OX=3661 GN=LOC111467024 PE=3 SV=1)

HSP 1 Score: 758 bits (1956), Expect = 1.63e-275
Identity = 393/405 (97.04%), Postives = 401/405 (99.01%), Query Frame = 0

Query: 1   MKPVKREPAASSRSNRSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60
           M+PVKREPAASSRSN+SKGGDAVDAANLRDVKRARVAREKENIG+GNMAEAEEGQQGLTK
Sbjct: 1   MRPVKREPAASSRSNQSKGGDAVDAANLRDVKRARVAREKENIGDGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFMN 180
           NSLVTSIKSQSNEGVTPSDFVSCLLKEFA+IEGGITSEENDPIS+SWKDIGLSVSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFADIEGGITSEENDPISISWKDIGLSVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240
           GHGC TMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL
Sbjct: 181 GHGCRTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360
           MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLN+APMPQ EA TDDSESA+LP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNTAPMPQAEAATDDSESAALP 360

Query: 361 TTPIRKLSRNRGLVMQTVVEDSPDND-AHSGPTAMLRCKKKLSQG 404
           TTPIRKLSRNRGLVMQ+VVEDSPDND AHSGPTAMLRCKKKLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQSVVEDSPDNDGAHSGPTAMLRCKKKLSQG 405

BLAST of Csor.00g049000 vs. ExPASy TrEMBL
Match: A0A1S3BQS4 (Non-structural maintenance of chromosomes element 4 OS=Cucumis melo OX=3656 GN=LOC103492497 PE=3 SV=1)

HSP 1 Score: 652 bits (1683), Expect = 7.03e-234
Identity = 343/409 (83.86%), Postives = 374/409 (91.44%), Query Frame = 0

Query: 1   MKPVKREPAASSRSNRSKGGD----AVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQ 60
           M+PVKREP  S+RS R++GGD    AVD A+LRD KR RVARE+EN GE NM+E E GQQ
Sbjct: 1   MRPVKREPTVSTRSKRNRGGDGGGGAVDGASLRDSKRERVARERENSGEENMSE-EVGQQ 60

Query: 61  GLTKRRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEAL 120
            LTKRRVLRSRYLAVIHEISERREDL+KD DKFNVIINEVEKLHEQVQKPREQVADAEAL
Sbjct: 61  DLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL 120

Query: 121 QDIANSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSS 180
           QDIANSLVTSI+SQSNEGVTPSDFVSCLL+EFA+I G I SEE + +SV+WKDIGLSVSS
Sbjct: 121 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQELVSVNWKDIGLSVSS 180

Query: 181 IFMNGHGCCTMLGPMSTQLKQRKTN-IARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSI 240
           IFMNG+GC TMLGPMS QLKQRKTN + RKRS+PTESSRPAEV+++GSE KTDTDKNM I
Sbjct: 181 IFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRSKPTESSRPAEVEDNGSEPKTDTDKNMGI 240

Query: 241 MFGILRRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAP 300
           MFGILRRNKTV+LEHLILNR SFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAP
Sbjct: 241 MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAP 300

Query: 301 AHSAIMSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSE 360
           AHSAIMSHEVNYSHFVFRFD+KDWK M+++VPVGEELMPHRNFLNS P+ +EEAT DDSE
Sbjct: 301 AHSAIMSHEVNYSHFVFRFDYKDWKPMVNMVPVGEELMPHRNFLNSVPVSEEEATADDSE 360

Query: 361 SASLPTTPIRKLSRNRGLVMQTVVEDSPDNDAHSGPTAMLRCKKKLSQG 404
           SASLPTTPIRKLSRNRGLVMQ+VVEDSPDN + SGPTA+LR K+KLSQG
Sbjct: 361 SASLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGPTAILRGKRKLSQG 408

BLAST of Csor.00g049000 vs. ExPASy TrEMBL
Match: A0A6J1HBJ2 (Non-structural maintenance of chromosomes element 4 OS=Cucurbita moschata OX=3662 GN=LOC111461359 PE=3 SV=1)

HSP 1 Score: 652 bits (1682), Expect = 1.07e-233
Identity = 344/407 (84.52%), Postives = 374/407 (91.89%), Query Frame = 0

Query: 1   MKPVKREPAASSRSNRSKGGD-AVDAANLRDVKRARV-AREKENIGEGNMAEAEEGQQGL 60
           M+PVKREPAASSRSNRS+GG  A DAA+LRDVKR +V +RE+E  G  N  E EEGQQ L
Sbjct: 1   MRPVKREPAASSRSNRSRGGAVAFDAASLRDVKREKVVSREREKSGVDNTTEEEEGQQEL 60

Query: 61  TKRRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQD 120
           +KRRVLRSRYLAVIHEISERREDL+KD DKFNVIINEVEKLHEQVQKPREQVADAEALQD
Sbjct: 61  SKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEALQD 120

Query: 121 IANSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIF 180
           IA+SLVTSI+SQSNEGVTPS+FVSCLL+EFA+I G ITSEEND +SVSWKDIGL VSSIF
Sbjct: 121 IASSLVTSIRSQSNEGVTPSNFVSCLLREFADINGNITSEENDLVSVSWKDIGLGVSSIF 180

Query: 181 MNGHGCCTMLGPMSTQLKQRKT-NIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMF 240
           MNGHGC TMLGPMS QLKQRKT NI+RKRSRPTES RPAEV ++GSEEKTDTDKNM IMF
Sbjct: 181 MNGHGCRTMLGPMSNQLKQRKTTNISRKRSRPTESYRPAEVGDNGSEEKTDTDKNMGIMF 240

Query: 241 GILRRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAH 300
           GILRRNKTV+LEHLILNR SFAQTVENLFALSFLVKDGRA ITIDKNGSHFVSPKNAPAH
Sbjct: 241 GILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAAITIDKNGSHFVSPKNAPAH 300

Query: 301 SAIMSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESA 360
           SAIMSHEVNYSHFVFRFDFKDWK M+D+VPVG+ELMPHRN LNSA +PQ EA+TDDSE+A
Sbjct: 301 SAIMSHEVNYSHFVFRFDFKDWKPMVDMVPVGDELMPHRNILNSAQVPQAEASTDDSEAA 360

Query: 361 SLPTTPIRKLSRNRGLVMQTVVEDSPDNDAHSGPTAMLRCKKKLSQG 404
           +LPTTPIRKLSRNRGLVMQ+VVEDSPDN AHS  T++LRCK+KL+QG
Sbjct: 361 ALPTTPIRKLSRNRGLVMQSVVEDSPDNGAHSRATSILRCKRKLNQG 407

BLAST of Csor.00g049000 vs. TAIR 10
Match: AT1G51130.1 (Nse4, component of Smc5/6 DNA repair complex )

HSP 1 Score: 382.1 bits (980), Expect = 5.5e-106
Identity = 215/406 (52.96%), Postives = 284/406 (69.95%), Query Frame = 0

Query: 2   KPVKREPAASSRSNRSKGGDAVDAANLRDVKRARVAR-EKENIGEGNMAEAEEGQQGLTK 61
           K VKRE  A+     +      +   LR VK+ +  + E +++        +E +QG++ 
Sbjct: 3   KTVKRESEATGGKREADD----EPEKLRSVKKEKQRKTEADSVRPDEPPPPQEEEQGISD 62

Query: 62  RRVLRSRYLAVIHEISERREDLTK-DSDKFNVIINEVEKLHEQVQKPREQVADAEALQDI 121
           RR+LRS+YL++ +EI++ ++DL K DSDKF+ IIN VE LH+QV+KPREQ+ADAEAL DI
Sbjct: 63  RRILRSKYLSLQNEINDCKDDLMKIDSDKFSRIINAVENLHQQVRKPREQIADAEALLDI 122

Query: 122 ANSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFM 181
           AN+L++S+KSQS  GV+P++FV+ L+  F     GI ++E   +S+ WKD+G +V S  +
Sbjct: 123 ANTLMSSVKSQSAHGVSPAEFVNALISGFGQGSLGIDTDETAQVSLKWKDLGFAVCSTVL 182

Query: 182 NGHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGI 241
              GC TMLGPM T+LKQRK    RKR++P E  RP EVD+  SEEKTDTDKNM+IMF I
Sbjct: 183 VSCGCSTMLGPMDTELKQRKRAPNRKRTKPGEGVRPDEVDDSQSEEKTDTDKNMTIMFNI 242

Query: 242 LRRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSA 301
           L + K V+LE+L+LNR SFAQTVENLFALSFL KDGR EI +DK+GSHF  P+NAP  + 
Sbjct: 243 LGKKKRVQLENLVLNRRSFAQTVENLFALSFLAKDGRVEIIVDKSGSHFAMPRNAPDANV 302

Query: 302 IMSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHR-NFLNSAPMPQEEATTDDSESAS 361
           +MS EV Y+HFVFR DFKDWKLM ++VP+GEELMPHR   + S+  P   A      +  
Sbjct: 303 VMSGEVIYNHFVFRLDFKDWKLMSEMVPLGEELMPHRQTAVASSSCPAASAPASADFTQD 362

Query: 362 LPTTPIRKLSRNRGLVMQ--TVVEDSPDNDAHSGPTAMLRCKKKLS 403
             TTPIRKLSRNRGLV+Q  TVVED+PD +   G     RCK++L+
Sbjct: 363 TQTTPIRKLSRNRGLVVQEETVVEDTPDKE---GDGTRRRCKRRLT 401

BLAST of Csor.00g049000 vs. TAIR 10
Match: AT3G20760.1 (Nse4, component of Smc5/6 DNA repair complex )

HSP 1 Score: 330.1 bits (845), Expect = 2.5e-90
Identity = 178/336 (52.98%), Postives = 242/336 (72.02%), Query Frame = 0

Query: 52  EEGQQGLTKRRVLRSRYLAVIHEISERREDLTK-DSDKFNVIINEVEKLHEQVQKPREQV 111
           EE +QG+  RR +RS+YLA+ H+I + ++DLTK DS+KFN IINEVE LH++V+KPREQ+
Sbjct: 53  EEEEQGIADRRAIRSQYLALTHKIKDAKDDLTKIDSNKFNSIINEVENLHQKVRKPREQI 112

Query: 112 ADAEALQDIANSLVTSIKSQS-NEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKD 171
           ADAEAL D+ NS+V+S+KSQS + GV+P++FV+ L+  F      I ++EN  +S+ WKD
Sbjct: 113 ADAEALLDLTNSVVSSVKSQSAHGGVSPAEFVNALINGFGKTSLRIDADENTQVSMKWKD 172

Query: 172 IGLSVSSIFMNGHGCCTMLGPMSTQLKQRKTNIA-RKRSRPTESSRPAEVDEDGSEEKTD 231
           +G +V S  +   GC TM+GPM +++KQRK+ +  RKR++P    +P EVD+  +E+K+D
Sbjct: 173 LGFTVCSTVLVSCGCTTMMGPMYSEMKQRKSRVGNRKRTKPGAGVKPEEVDDTEAEKKSD 232

Query: 232 TDKNMSIMFGILRRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHF 291
           TD NM++MF ILR+NK V++E+L+LNR SFAQT EN+FALSFLVKDGR EIT+D NGSHF
Sbjct: 233 TDNNMAVMFNILRKNKRVKIENLVLNRKSFAQTAENMFALSFLVKDGRVEITVDNNGSHF 292

Query: 292 VSPKNAPAHSAIMSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEE 351
           V P+NAPA + ++S EV Y+HFV RFD+KDW+ M  +V VGEELMP R          E 
Sbjct: 293 VEPRNAPAANLVLSGEVAYNHFVLRFDYKDWEPMSKMVAVGEELMPLR----------ET 352

Query: 352 ATTDDSESASLPTTPIRKLSRNRGLVMQ--TVVEDS 383
               DS           +LSR +G V+Q  TVVEDS
Sbjct: 353 KVAQDS----------IELSRKQGSVIQEETVVEDS 368

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C6897.8e-10552.96Non-structural maintenance of chromosomes element 4 homolog A OS=Arabidopsis tha... [more]
F4JET13.5e-8952.98Non-structural maintenance of chromosomes element 4 homolog B OS=Arabidopsis tha... [more]
Q2TBI11.2e-1223.29Non-structural maintenance of chromosomes element 4 homolog A OS=Bos taurus OX=9... [more]
Q9NXX63.5e-1222.67Non-structural maintenance of chromosomes element 4 homolog A OS=Homo sapiens OX... [more]
Q6BDR81.0e-1124.21Non-structural maintenance of chromosome element 4 OS=Schizosaccharomyces pombe ... [more]
Match NameE-valueIdentityDescription
KAG6588168.11.64e-284100.00Non-structural maintenance of chromosomes element 4-like A, partial [Cucurbita a... [more]
KAG7022067.16.66e-28499.75Non-structural maintenance of chromosomes element 4-like A [Cucurbita argyrosper... [more]
XP_022933648.11.18e-27798.02non-structural maintenance of chromosomes element 4 homolog A-like isoform X2 [C... [more]
XP_022933647.11.37e-27798.02non-structural maintenance of chromosomes element 4 homolog A-like isoform X1 [C... [more]
XP_023530878.11.67e-27597.04non-structural maintenance of chromosomes element 4 homolog A-like isoform X3 [C... [more]
Match NameE-valueIdentityDescription
A0A6J1F5E65.71e-27898.02Non-structural maintenance of chromosomes element 4 OS=Cucurbita moschata OX=366... [more]
A0A6J1F0F06.64e-27898.02Non-structural maintenance of chromosomes element 4 OS=Cucurbita moschata OX=366... [more]
A0A6J1HSJ21.63e-27597.04Non-structural maintenance of chromosomes element 4 OS=Cucurbita maxima OX=3661 ... [more]
A0A1S3BQS47.03e-23483.86Non-structural maintenance of chromosomes element 4 OS=Cucumis melo OX=3656 GN=L... [more]
A0A6J1HBJ21.07e-23384.52Non-structural maintenance of chromosomes element 4 OS=Cucurbita moschata OX=366... [more]
Match NameE-valueIdentityDescription
AT1G51130.15.5e-10652.96Nse4, component of Smc5/6 DNA repair complex [more]
AT3G20760.12.5e-9052.98Nse4, component of Smc5/6 DNA repair complex [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014854Non-structural maintenance of chromosome element 4, C-terminalPFAMPF08743Nse4_Ccoord: 246..335
e-value: 4.4E-25
score: 87.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 340..404
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 204..228
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 196..228
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..57
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 343..365
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availablePANTHERPTHR16140:SF18NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4coord: 25..401
IPR027786Nse4/EID familyPANTHERPTHR16140UNCHARACTERIZEDcoord: 25..401

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g049000.m01Csor.00g049000.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006310 DNA recombination
biological_process GO:0006281 DNA repair
cellular_component GO:0005634 nucleus
cellular_component GO:0030915 Smc5-Smc6 complex