Csor.00g044840 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g044840
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	High-affinity nitrate transporter
Location	Csor_Chr18: 1096377 .. 1097121 (+)
RNA-Seq Expression	Csor.00g044840
Synteny	Csor.00g044840

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintronterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGCTCATGGGTTTCCTCTGACTGCTCTGTTTTTGCTCTGTTTCTTGGGGTTCTCTCATGGACATATCCATCTTTCTTCTATCCCAAGAAATTTGGAGGTCACTGCTTCTCCAAAATCTGGACAAGGTAAGAACTTTCAGCTCCCATGACTCAAATTTCTGATGCTTCCAACACAGATCTGAATCAAAAACTGTTAGTTTAGCGATTTTTAACATCTGGGTTTTTGTTTTTTTTGCAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGTCATGGGTTCTTAACGGAGCTGCAAAAGTTGGGTCGGATTTGGCTTACAAATCTGTGAAGGTGAAGCTCTGCTATGCGCCGGTGAGCCAAGTGGACCGTGCGTGGAGAAAAACAGAGGACGATCTTGCAAAGGACAAGACTTGTCAATTCCAAATCGTGGAGAAGCCGTACAATCCGTCGAACAAGACGGTTCAAAAGGCTGAGTGGACGGTGGAGCGTGACACTCCGACGGCGACTTTCTTCGTGCGTGCTTACGCGTTCAATTCCGCCGGCGATGAAGTGGCGTACGGACAAACGACAGACGAAAAGAAAGGCACAAATTTGTTCCAAGTTGAAGCTATCACTGGCCGCCATATCTCTCTCGATATTGCTTCTGGTTGTTTCAGCGCTTTCTCTGTGCTGTCTTTGACCGGATTCTTCATCGCCGGTAAGAGGCAGGCGAAGAAGGCGGCGGCGGCGGAGGGGCACTAA

mRNA sequence

ATGGCCGCTCATGGGTTTCCTCTGACTGCTCTGTTTTTGCTCTGTTTCTTGGGGTTCTCTCATGGACATATCCATCTTTCTTCTATCCCAAGAAATTTGGAGGTCACTGCTTCTCCAAAATCTGGACAAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGTCATGGGTTCTTAACGGAGCTGCAAAAGTTGGGTCGGATTTGGCTTACAAATCTGTGAAGGTGAAGCTCTGCTATGCGCCGGTGAGCCAAGTGGACCGTGCGTGGAGAAAAACAGAGGACGATCTTGCAAAGGACAAGACTTGTCAATTCCAAATCGTGGAGAAGCCGTACAATCCGTCGAACAAGACGGTTCAAAAGGCTGAGTGGACGGTGGAGCGTGACACTCCGACGGCGACTTTCTTCGTGCGTGCTTACGCGTTCAATTCCGCCGGCGATGAAGTGGCGTACGGACAAACGACAGACGAAAAGAAAGGCACAAATTTGTTCCAAGTTGAAGCTATCACTGGCCGCCATATCTCTCTCGATATTGCTTCTGGTTGTTTCAGCGCTTTCTCTGTGCTGTCTTTGACCGGATTCTTCATCGCCGGTAAGAGGCAGGCGAAGAAGGCGGCGGCGGCGGAGGGGCACTAA

Coding sequence (CDS)

ATGGCCGCTCATGGGTTTCCTCTGACTGCTCTGTTTTTGCTCTGTTTCTTGGGGTTCTCTCATGGACATATCCATCTTTCTTCTATCCCAAGAAATTTGGAGGTCACTGCTTCTCCAAAATCTGGACAAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGTCATGGGTTCTTAACGGAGCTGCAAAAGTTGGGTCGGATTTGGCTTACAAATCTGTGAAGGTGAAGCTCTGCTATGCGCCGGTGAGCCAAGTGGACCGTGCGTGGAGAAAAACAGAGGACGATCTTGCAAAGGACAAGACTTGTCAATTCCAAATCGTGGAGAAGCCGTACAATCCGTCGAACAAGACGGTTCAAAAGGCTGAGTGGACGGTGGAGCGTGACACTCCGACGGCGACTTTCTTCGTGCGTGCTTACGCGTTCAATTCCGCCGGCGATGAAGTGGCGTACGGACAAACGACAGACGAAAAGAAAGGCACAAATTTGTTCCAAGTTGAAGCTATCACTGGCCGCCATATCTCTCTCGATATTGCTTCTGGTTGTTTCAGCGCTTTCTCTGTGCTGTCTTTGACCGGATTCTTCATCGCCGGTAAGAGGCAGGCGAAGAAGGCGGCGGCGGCGGAGGGGCACTAA

Protein sequence

MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH

Homology

[Close]

BLAST of Csor.00g044840 vs. ExPASy Swiss-Prot
Match: Q9FGS5 (High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 6.6e-47
Identity = 99/175 (56.57%), Postives = 126/175 (72.00%), Query Frame = 0


Query: 33  LEVTASPK---SGQVLKAGVDKISVSWVLNGAAKVGS--DLAYKSVKVKLCYAPVSQVDR 92
           L+VT  P     G VL AG D ++++W L   + +GS  +  +K +KVKLCYAP SQVDR
Sbjct: 37  LDVTTKPSREGPGVVLDAGKDTLNITWTL---SSIGSKREAEFKIIKVKLCYAPPSQVDR 96

Query: 93  AWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDE 152
            WRKT D+L KDKTC  +I+ KPY   +KT+Q   WT+ERD PT T+FVRAYA ++ G E
Sbjct: 97  PWRKTHDELFKDKTCPHKIIAKPY---DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHE 156

Query: 153 VAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAK 203
           VAYGQ+TD+ K TNLF V+AI+GRH SLDIAS CFS FSV++L  FF+  KR+AK
Sbjct: 157 VAYGQSTDDAKKTNLFSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Csor.00g044840 vs. ExPASy Swiss-Prot
Match: Q9SB67 (High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 9.5e-46
Identity = 100/207 (48.31%), Postives = 137/207 (66.18%), Query Frame = 0


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIH---LSSIPRNLEVTASP-KSGQVLKAGVDKISVSW 60
           MA H     +L +   +  S G       + +  ++EVTA P K   VL+AG D ++++W
Sbjct: 1   MAIHTLLFVSLLIFSLIESSSGGKKDRLFTDLQNSIEVTAKPVKDSGVLEAGKDMVTITW 60

Query: 61  VL-NGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSN 120
            L + +AKV +D A+K+++VKLCYAP+SQVDR WRKT++ L KD++C  +IV K Y   +
Sbjct: 61  KLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAY---D 120

Query: 121 KTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISL 180
           KT Q  +WT+  D PT T+FVRAY  +  G EVAYGQ+TDE + TNLF V AI+G H+ L
Sbjct: 121 KTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGL 180

Query: 181 DIASGCFSAFSVLSLTGFFIAGKRQAK 203
           DIAS  FS FSV+SL  FF+  KR+AK
Sbjct: 181 DIASTFFSVFSVVSLFVFFVMEKRKAK 204

[Close]

BLAST of Csor.00g044840 vs. ExPASy Swiss-Prot
Match: Q6ZI50 (High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.1 PE=1 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.4e-41
Identity = 86/180 (47.78%), Postives = 119/180 (66.11%), Query Frame = 0


Query: 24  IHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVS 83
           + LS +P+ L V  SPK G+V+ AG + ++V+W LN +   G+D A+KSVKVKLCYAP S
Sbjct: 34  VFLSKLPKALVVGVSPKHGEVVHAGENTVTVTWSLNTSEPAGADAAFKSVKVKLCYAPAS 93

Query: 84  QVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNS 143
           + DR WRK  DDL KDK CQF++  +PY        + ++ V RD PTA++FVRAYA ++
Sbjct: 94  RTDRGWRKASDDLHKDKACQFKVTVQPYAAG---AGRFDYVVARDIPTASYFVRAYAVDA 153

Query: 144 AGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKK 203
           +G EVAYGQ++ +      F V  ITG H SL +A+G FS FS+ +L  FF+  KR+  K
Sbjct: 154 SGTEVAYGQSSPDA----AFDVAGITGIHASLKVAAGVFSTFSIAALAFFFVVEKRKKDK 206

[Close]

BLAST of Csor.00g044840 vs. ExPASy Swiss-Prot
Match: Q7XK12 (Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.2 PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 5.9e-40
Identity = 96/210 (45.71%), Postives = 127/210 (60.48%), Query Frame = 0


Query: 4   HGFPLTALFLLCFLGFSH-------GHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSW 63
           H   L  L LL  LG  H           LS++ + L V ASP++GQVL AG D I+V+W
Sbjct: 9   HRVFLPLLLLLVVLGACHVTPAAAAAGARLSALAKALVVEASPRAGQVLHAGEDAITVTW 68

Query: 64  VLN---GAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNP 123
            LN    AA  G+D  YK+VKV LCYAP SQV R WRK  DDL+KDK CQF+I ++PY+ 
Sbjct: 69  SLNATAAAAAAGADAGYKAVKVTLCYAPASQVGRGWRKAHDDLSKDKACQFKIAQQPYDG 128

Query: 124 SNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHI 183
           +     K E+TV RD PTA+++VRAYA +++G  VAYG+T         F V  ITG   
Sbjct: 129 AG----KFEYTVARDVPTASYYVRAYALDASGARVAYGETAPSAS----FAVAGITGVTA 188

Query: 184 SLDIASGCFSAFSVLSLTGFFIAGKRQAKK 204
           S+++A+G  SAFSV +L  F +   ++  K
Sbjct: 189 SIEVAAGVLSAFSVAALAVFLVLENKKKNK 210

[Close]

BLAST of Csor.00g044840 vs. NCBI nr
Match: XP_022954957.1 (high-affinity nitrate transporter 3.1-like [Cucurbita moschata] >KAG6573089.1 High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 417 bits (1071), Expect = 7.72e-147
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 0


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG
Sbjct: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
           AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK
Sbjct: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
           AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG
Sbjct: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 210
           CFSAFSVLSLTGFFIAGKRQAKKAAAAEGH
Sbjct: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 210

[Close]

BLAST of Csor.00g044840 vs. NCBI nr
Match: XP_023542774.1 (high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 412 bits (1060), Expect = 3.67e-145
Identity = 206/210 (98.10%), Postives = 210/210 (100.00%), Query Frame = 0


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           MAAHGFPLTALFLLCFLGFSHGHIHLSSIPR+LEVTASPKSGQVLKAGVDKISVSWVLNG
Sbjct: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRDLEVTASPKSGQVLKAGVDKISVSWVLNG 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
           AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK
Sbjct: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
           AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQ+EAI+GRH+SLDIASG
Sbjct: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQIEAISGRHVSLDIASG 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 210
           CFSAFSVLSLTGFFIAGKRQAKKAAAAEGH
Sbjct: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 210

[Close]

BLAST of Csor.00g044840 vs. NCBI nr
Match: XP_022994709.1 (high-affinity nitrate transporter 3.1-like [Cucurbita maxima])

HSP 1 Score: 405 bits (1042), Expect = 2.04e-142
Identity = 204/210 (97.14%), Postives = 207/210 (98.57%), Query Frame = 0


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           MAAHGFPLTALFLLCFLGFSHGHIHLSSIPR LEVTASPKSGQVLKAGVDKISVSWVLNG
Sbjct: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRTLEVTASPKSGQVLKAGVDKISVSWVLNG 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
           AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK
Sbjct: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
           AEWTV+RDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAI+GRH SLDIAS 
Sbjct: 121 AEWTVKRDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAISGRHASLDIASA 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 210
           CFSAFSVLSLTGFFIAGKR+AKKAAAAEGH
Sbjct: 181 CFSAFSVLSLTGFFIAGKRKAKKAAAAEGH 210

[Close]

BLAST of Csor.00g044840 vs. NCBI nr
Match: KAG7012276.1 (High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 348 bits (893), Expect = 6.39e-120
Identity = 175/176 (99.43%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 35  VTASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 94
           +TASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED
Sbjct: 21  ITASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 80

Query: 95  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT 154
           DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT
Sbjct: 81  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT 140

Query: 155 DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 210
           DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH
Sbjct: 141 DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 196

[Close]

BLAST of Csor.00g044840 vs. NCBI nr
Match: KAG6584111.1 (High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019702.1 High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 348 bits (894), Expect = 8.79e-120
Identity = 173/208 (83.17%), Postives = 187/208 (89.90%), Query Frame = 0


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           M+A+GF L ALFLLCFLGFSHGHIH SS+PR+LEVTASPK GQVLKAGVDKISV+WVLNG
Sbjct: 7   MSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
             K GSD +YK+V+ KLCYAPVSQVDRAWRKTEDDL+KDKTCQF IV KPYNP NKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
            EWTV RD PT TFFVRAYAFNSAG+EV YGQTTD KKGTNLFQ+EAITGRHISLDIAS 
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAAE 208
           CFSAFSVLSL+GFF+AGKR+AKKA A E
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEAPE 214

[Close]

BLAST of Csor.00g044840 vs. ExPASy TrEMBL
Match: A0A6J1GSA0 (High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111457061 PE=3 SV=1)

HSP 1 Score: 417 bits (1071), Expect = 3.74e-147
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 0


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG
Sbjct: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
           AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK
Sbjct: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
           AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG
Sbjct: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 210
           CFSAFSVLSLTGFFIAGKRQAKKAAAAEGH
Sbjct: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 210

[Close]

BLAST of Csor.00g044840 vs. ExPASy TrEMBL
Match: A0A6J1K3M2 (High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111490359 PE=3 SV=1)

HSP 1 Score: 405 bits (1042), Expect = 9.87e-143
Identity = 204/210 (97.14%), Postives = 207/210 (98.57%), Query Frame = 0


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           MAAHGFPLTALFLLCFLGFSHGHIHLSSIPR LEVTASPKSGQVLKAGVDKISVSWVLNG
Sbjct: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRTLEVTASPKSGQVLKAGVDKISVSWVLNG 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
           AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK
Sbjct: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
           AEWTV+RDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAI+GRH SLDIAS 
Sbjct: 121 AEWTVKRDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAISGRHASLDIASA 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 210
           CFSAFSVLSLTGFFIAGKR+AKKAAAAEGH
Sbjct: 181 CFSAFSVLSLTGFFIAGKRKAKKAAAAEGH 210

[Close]

BLAST of Csor.00g044840 vs. ExPASy TrEMBL
Match: A0A6J1EDS1 (High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 PE=3 SV=1)

HSP 1 Score: 347 bits (891), Expect = 1.22e-119
Identity = 172/206 (83.50%), Postives = 185/206 (89.81%), Query Frame = 0


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           M+A+GF L ALFL CFLGFSHGHIH SS+PR+LEVTASPK GQVLKAGVDKISV+WVLNG
Sbjct: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
             K GSD +YK+V+ KLCYAPVSQVDRAWRKTEDDL+KDKTCQF IV KPYNP NKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
            EWTV RD PT TFFVRAYAFNSAGDEV YGQTTD KKGTNLFQ+EAITGRHISLDIAS 
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAA 206
           CFSAFSVLSL+GFF+AGKR+AKKA A
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEA 212

[Close]

BLAST of Csor.00g044840 vs. ExPASy TrEMBL
Match: A0A6J1KMC9 (High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111495476 PE=3 SV=1)

HSP 1 Score: 342 bits (876), Expect = 2.35e-117
Identity = 168/206 (81.55%), Postives = 185/206 (89.81%), Query Frame = 0


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           M+A+GF L ALFL CFLGFSHGHIH SS+PR+LEVTASPK GQVLKAGVDKISV+WVLNG
Sbjct: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
             K GSD +YK+V+ KLCYAPVSQVDRAWRKTEDDL+KDKTCQF IV KPYNP+NKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
            EWTV RD PT TFFVRAYAFNSAG+EV YGQT+D KKGTNLFQ++AITGRHISLDIAS 
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASV 186

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAA 206
           CFSAFSVLSL+GFF+AGKR+ KKA A
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKTKKAEA 212

[Close]

BLAST of Csor.00g044840 vs. ExPASy TrEMBL
Match: A0A5A7ULV7 (High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004000 PE=3 SV=1)

HSP 1 Score: 335 bits (860), Expect = 6.00e-115
Identity = 166/207 (80.19%), Postives = 181/207 (87.44%), Query Frame = 0


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           MA++GF L ALFL CFLGFS G+IHLSS+PR LEVTASPK GQVLKAGVDKI V+WVLNG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
             K GSD +YK++K KLCYAPVSQVDRAWRKTEDDL KDKTCQF IVEKPYNP+NKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
            EWTVERD PT TFFVRAY FNSAG EVAYGQTTD+KKGTNLFQ+E+ITGRH+SLDIAS 
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAA 207
           CFSAFSV+SL GFF   KR+AKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

[Close]

BLAST of Csor.00g044840 vs. TAIR 10
Match: AT5G50200.3 (nitrate transmembrane transporters )

HSP 1 Score: 188.7 bits (478), Expect = 4.7e-48
Identity = 99/175 (56.57%), Postives = 126/175 (72.00%), Query Frame = 0


Query: 33  LEVTASPK---SGQVLKAGVDKISVSWVLNGAAKVGS--DLAYKSVKVKLCYAPVSQVDR 92
           L+VT  P     G VL AG D ++++W L   + +GS  +  +K +KVKLCYAP SQVDR
Sbjct: 37  LDVTTKPSREGPGVVLDAGKDTLNITWTL---SSIGSKREAEFKIIKVKLCYAPPSQVDR 96

Query: 93  AWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDE 152
            WRKT D+L KDKTC  +I+ KPY   +KT+Q   WT+ERD PT T+FVRAYA ++ G E
Sbjct: 97  PWRKTHDELFKDKTCPHKIIAKPY---DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHE 156

Query: 153 VAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAK 203
           VAYGQ+TD+ K TNLF V+AI+GRH SLDIAS CFS FSV++L  FF+  KR+AK
Sbjct: 157 VAYGQSTDDAKKTNLFSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Csor.00g044840 vs. TAIR 10
Match: AT5G50200.2 (nitrate transmembrane transporters )

HSP 1 Score: 188.7 bits (478), Expect = 4.7e-48
Identity = 99/175 (56.57%), Postives = 126/175 (72.00%), Query Frame = 0


Query: 33  LEVTASPK---SGQVLKAGVDKISVSWVLNGAAKVGS--DLAYKSVKVKLCYAPVSQVDR 92
           L+VT  P     G VL AG D ++++W L   + +GS  +  +K +KVKLCYAP SQVDR
Sbjct: 37  LDVTTKPSREGPGVVLDAGKDTLNITWTL---SSIGSKREAEFKIIKVKLCYAPPSQVDR 96

Query: 93  AWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDE 152
            WRKT D+L KDKTC  +I+ KPY   +KT+Q   WT+ERD PT T+FVRAYA ++ G E
Sbjct: 97  PWRKTHDELFKDKTCPHKIIAKPY---DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHE 156

Query: 153 VAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAK 203
           VAYGQ+TD+ K TNLF V+AI+GRH SLDIAS CFS FSV++L  FF+  KR+AK
Sbjct: 157 VAYGQSTDDAKKTNLFSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Csor.00g044840 vs. TAIR 10
Match: AT5G50200.1 (nitrate transmembrane transporters )

HSP 1 Score: 188.7 bits (478), Expect = 4.7e-48
Identity = 99/175 (56.57%), Postives = 126/175 (72.00%), Query Frame = 0


Query: 33  LEVTASPK---SGQVLKAGVDKISVSWVLNGAAKVGS--DLAYKSVKVKLCYAPVSQVDR 92
           L+VT  P     G VL AG D ++++W L   + +GS  +  +K +KVKLCYAP SQVDR
Sbjct: 37  LDVTTKPSREGPGVVLDAGKDTLNITWTL---SSIGSKREAEFKIIKVKLCYAPPSQVDR 96

Query: 93  AWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDE 152
            WRKT D+L KDKTC  +I+ KPY   +KT+Q   WT+ERD PT T+FVRAYA ++ G E
Sbjct: 97  PWRKTHDELFKDKTCPHKIIAKPY---DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHE 156

Query: 153 VAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAK 203
           VAYGQ+TD+ K TNLF V+AI+GRH SLDIAS CFS FSV++L  FF+  KR+AK
Sbjct: 157 VAYGQSTDDAKKTNLFSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Csor.00g044840 vs. TAIR 10
Match: AT4G24730.4 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 184.9 bits (468), Expect = 6.7e-47
Identity = 100/207 (48.31%), Postives = 137/207 (66.18%), Query Frame = 0


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIH---LSSIPRNLEVTASP-KSGQVLKAGVDKISVSW 60
           MA H     +L +   +  S G       + +  ++EVTA P K   VL+AG D ++++W
Sbjct: 23  MAIHTLLFVSLLIFSLIESSSGGKKDRLFTDLQNSIEVTAKPVKDSGVLEAGKDMVTITW 82

Query: 61  VL-NGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSN 120
            L + +AKV +D A+K+++VKLCYAP+SQVDR WRKT++ L KD++C  +IV K Y   +
Sbjct: 83  KLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAY---D 142

Query: 121 KTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISL 180
           KT Q  +WT+  D PT T+FVRAY  +  G EVAYGQ+TDE + TNLF V AI+G H+ L
Sbjct: 143 KTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGL 202

Query: 181 DIASGCFSAFSVLSLTGFFIAGKRQAK 203
           DIAS  FS FSV+SL  FF+  KR+AK
Sbjct: 203 DIASTFFSVFSVVSLFVFFVMEKRKAK 226

[Close]

BLAST of Csor.00g044840 vs. TAIR 10
Match: AT4G24730.1 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 178.3 bits (451), Expect = 6.3e-45
Identity = 96/184 (52.17%), Postives = 129/184 (70.11%), Query Frame = 0


Query: 20  SHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVL-NGAAKVGSDLAYKSVKVKLC 79
           SHG  H     R+LE  A+ +   VL+AG D ++++W L + +AKV +D A+K+++VKLC
Sbjct: 265 SHGVHH-----RSLE--AALECPPVLEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLC 324

Query: 80  YAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRA 139
           YAP+SQVDR WRKT++ L KD++C  +IV K Y   +KT Q  +WT+  D PT T+FVRA
Sbjct: 325 YAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAY---DKTPQSLDWTIGLDIPTGTYFVRA 384

Query: 140 YAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGK 199
           Y  +  G EVAYGQ+TDE + TNLF V AI+G H+ LDIAS  FS FSV+SL  FF+  K
Sbjct: 385 YGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEK 438

Query: 200 RQAK 203
           R+AK
Sbjct: 445 RKAK 438

The following BLAST results are available for this feature:

BLAST of Csor.00g044840 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FGS5	6.6e-47	56.57	High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 ...	[more]
Q9SB67	9.5e-46	48.31	High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 ...	[more]
Q6ZI50	2.4e-41	47.78	High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. ...	[more]
Q7XK12	5.9e-40	45.71	Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativ...	[more]

BLAST of Csor.00g044840 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022954957.1	7.72e-147	100.00	high-affinity nitrate transporter 3.1-like [Cucurbita moschata] >KAG6573089.1 Hi...	[more]
XP_023542774.1	3.67e-145	98.10	high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022994709.1	2.04e-142	97.14	high-affinity nitrate transporter 3.1-like [Cucurbita maxima]	[more]
KAG7012276.1	6.39e-120	99.43	High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. ar...	[more]
KAG6584111.1	8.79e-120	83.17	High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. so...	[more]

BLAST of Csor.00g044840 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GSA0	3.74e-147	100.00	High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111457061 ...	[more]
A0A6J1K3M2	9.87e-143	97.14	High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111490359 PE...	[more]
A0A6J1EDS1	1.22e-119	83.50	High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 ...	[more]
A0A6J1KMC9	2.35e-117	81.55	High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111495476 PE...	[more]
A0A5A7ULV7	6.00e-115	80.19	High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]

BLAST of Csor.00g044840 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50200.3	4.7e-48	56.57	nitrate transmembrane transporters	[more]
AT5G50200.2	4.7e-48	56.57	nitrate transmembrane transporters	[more]
AT5G50200.1	4.7e-48	56.57	nitrate transmembrane transporters	[more]
AT4G24730.4	6.7e-47	48.31	Calcineurin-like metallo-phosphoesterase superfamily protein	[more]
AT4G24730.1	6.3e-45	52.17	Calcineurin-like metallo-phosphoesterase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016605	High-affinity nitrate transporter	PFAM	PF16974	NAR2	coord: 27..200 e-value: 3.0E-73 score: 244.9
IPR016605	High-affinity nitrate transporter	PIRSF	PIRSF012939	NAR2	coord: 1..205 e-value: 1.2E-81 score: 270.0
IPR016605	High-affinity nitrate transporter	PANTHER	PTHR34806	HIGH-AFFINITY NITRATE TRANSPORTER 3.2	coord: 8..204
None	No IPR available	PANTHER	PTHR34806:SF1	HIGH-AFFINITY NITRATE TRANSPORTER 3.2	coord: 8..204

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g044840.m01	Csor.00g044840.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042128	nitrate assimilation
biological_process	GO:0015706	nitrate transport
biological_process	GO:0010167	response to nitrate
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0015112	nitrate transmembrane transporter activity