Csor.00g041690 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g041690
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionProteasome inhibitor PI31 subunit
LocationCsor_Chr07: 5523080 .. 5526068 (+)
RNA-Seq ExpressionCsor.00g041690
SyntenyCsor.00g041690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGAACGAGCAATCGGTAATGGCCGTCATCAGGGCAACTAGGGCTAGCTTTCGTAACGATTACGACAAGGTCGCGTTTGCAGTTCACGCCACATTTCTCGCCTCCGGCTTCGTGCTCACTGCCACCGGACCTTCTGCTTTTACTGAAGCCGCCTTTTCTTCTCCCTCTACGGGTATGAGTTCTATGTATATTGCCAACGCCTTAATTTTGTTTCATAGCTTAGTATTTCTGAGTATTGGATTGGGGTTTAGATGAAGTTAGTATCGAGGGCTGGAACGAATTGGAGGATGAGTACGCTTACGTTTACGCAAACCCTGAAAGGGATTCTAATTGGAAGAAAATGATAGTGAAGTGCCTGGTGATGAACGGGAAATTGCTTATTGATGCCTTGACTGATAACAATTCGGACCCGCTGCATCTTGAAATTGAGTACGCTACACTCTCCTAGATCTATGCATCTTGGGAAATTGGTACTTTTCTTGCTTCGATGTTGAGTTTTTGTTAATTTAAATTTTAGCGTTGAAGAAATCGTCAATCCCAATGGGGGAAGTAGCTATACAACTCAGTACAGAAATTTGGATAAACTGGTGGAACGCTTGGACTTGGGAATATTGAGTAAATTGCATGGTTCTTCAAAAGCTACGAGAAGTAGTTCTACAAGGTAGTGATAGTTCAGATAATCAATACGTACGATCATTTTAATTATTCATTTTCCTGATGTTATCGAGAGGGATTTTGGTTGAACTGATTATGATAACTTTTTTTCACATTCTTATTTATTGGAGCGTTGTCACTATGGATTGAATTTAACTTAGTATTAGGTTTTGAAAGCCTCACACACTTTGTTCATGCTGCTAAATATTGAGTTCATGTTCATTGGTTATTTGTTTAAGATTGTTTTAGGACGTTGCAATATGCTCAGCATCTTAATTTTTATTTTATCCATCATGCGATTGAGTTACGAATTTGTAACAATTTAGAGAGATTTTTAAAGCTTCGATCATGTTTATGGGACTCAAATGACAATTTGTTGGTCCCATTTCTAGTATTTATGTGACCTGATGTGGAAAAAATCTATGCCTTTAGCAATCATTCTTTAATTTGCTTCTTTGCACTCTATATGCCTTTTGTGATTTGCAGGTCTGAGAGTAGTGAAAGATCATCTGCTGCTGTAAATGAACCTACTTCTGGAATCCCTGAGAATCATAGCCCCCCATCGAATATGCCTATGTTTGGAATTCCTGAGCGTCCTGGTCCACAAATCGATCCTTCAGGGTCTCTCTCTCTCTGTCCACATATGCACAGTCCCAAAATATTATGACATAAGTTTAGAAATATGTTGGGTCTTGACTTGAAGTTTTTAACTATTAAGTACTACTACCATGATAGGTTTGTTGGGTTCTATTATCACTGTCTCCATCATTTGGATTTGCAGGGTTCTAACCATATTTTGGATGCGATTTTTCTTTTTGTTTTCTTCATAATACATTGTTTTCTCCTTCATGTGTAGAACATGTGAGGGAGAGGTAAGATCTGTGTGATTTGAGTTTCTTGGGCTTGGGCACCATGCTTGTGTTATATATGATAGTGATCACTGGTCATGCTTCCATTTCATGGGATAGTTTTCTTCCCGAGAGACTCAACTCATCGGTTCCTTTTCTGTTAGCACAATACGGTTCCTCCCACTCACATAACTAATTCCCAAAAGAAAAAAGAAGCCAATTTAAAATTCTTTAATATCATTCTTATTTTTCTGAGCTATATTGCTACTTGAAAAATGAAAATGGTTCCACTTAATGTTCCAGAGTTGTTTTTGGCTTTTGCTCAATTATTGGGATGGTCGAATCGTTGGTAGTATTTATTGGGAAGAATTTTGTGTCTTTTGTCCAATCCTTCCTTAACAGATGACATTAACTACCAATCTAGAAACTTGTTTAATATTCAATGTCTACAAATAGTCTGTATCACATGAGTGAATTGTCATAGTCTGATCGTGTTTGATTTTGTTACTATCTAGAGTTGTAATTCCACCTATTAATCCCTTTGGTGGTGGTGATCTTTTCCCTGGACCTGGTGCTGGAATGTACCCCAATAGGTAAATTATTTAAATCCATCGATCATCTGTGTATATGAGATTGCGGTAAGTGCAAAATATCAGGCTAATTTGTTTTGTTTTGTTCTTGTTTTTTTATTCTTCCAATAATTGATTTCAGAGGTGGTTTCAGCGGGGATGGGAGCATGCTTTTAGGTTTGTGAATTTTTGTTCTCTTCTAGACTTCTAGTGTTAGATTGATAGCAAGTGTAATAAGTTCACTCTTTTATTATGCAGGACCTAATGACCCGCGATGGTTTGGAATTGGTAGAGGACCTGGTTTTCAAGGCGGGCAGCCGTGAGTCGATTAGCTCTCTCATACACGCCCACTCCCACACGCTCACCCTTGCACTATGATTGCTTCTAATTCTGAGTTTTTTATTATTTTTTTAAACAGGGGCGTTCCTCCAGGTGCTCGTTTTGATCCATATGGTCCACCTGGTGTTCCTGGGTTTGAACCAAATCGGTTTGTTAGGTATTTGGGGTCTTCCTAAGGCTTAAACTTACTTGTTGGATTAATAATTTTCTACTAGCGTTACCATGTTCTGAATGCTGTGACAACTACTAAAGTCAAACCATGTTTCTTTTCATTTTAAATTAGGATAGGTCATCCATGAAAAAACAAGTTAGGGTCATTAGCCCATATGTCTTTCATAGAAAACAACTCGAGTAATATAAGGAAAACATTTAAGAAAAGTTAGGGTCAGTTTGGAATGACTTTCTAAGTATGTAAAAGGAGTTTTGGATGGTTAAAATCGTATTTTTAATTTGGTGAGGACCAACAAGACTAACTTGGACTTTTCTACTTCGGATTCTTACAGAAATCCTAGGAGACCAGGAGGAGGGACTCACCCGGACCTGGAGCATTTTGGAGGCGGTTCAGATTTTATATAG

mRNA sequence

ATGGTGAACGAGCAATCGGTAATGGCCGTCATCAGGGCAACTAGGGCTAGCTTTCGTAACGATTACGACAAGGTCGCGTTTGCAGTTCACGCCACATTTCTCGCCTCCGGCTTCGTGCTCACTGCCACCGGACCTTCTGCTTTTACTGAAGCCGCCTTTTCTTCTCCCTCTACGGATGAAGTTAGTATCGAGGGCTGGAACGAATTGGAGGATGAGTACGCTTACGTTTACGCAAACCCTGAAAGGGATTCTAATTGGAAGAAAATGATAGTGAAGTGCCTGGTGATGAACGGGAAATTGCTTATTGATGCCTTGACTGATAACAATTCGGACCCGCTGCATCTTGAAATTGACGTTGAAGAAATCGTCAATCCCAATGGGGGAAGTAGCTATACAACTCAGTACAGAAATTTGGATAAACTGGTGGAACGCTTGGACTTGGGAATATTGAGTAAATTGCATGGTTCTTCAAAAGCTACGAGAAGTAGTTCTACAAGGTCTGAGAGTAGTGAAAGATCATCTGCTGCTGTAAATGAACCTACTTCTGGAATCCCTGAGAATCATAGCCCCCCATCGAATATGCCTATGTTTGGAATTCCTGAGCGTCCTGGTCCACAAATCGATCCTTCAGGAGTTGTAATTCCACCTATTAATCCCTTTGGTGGTGGTGATCTTTTCCCTGGACCTGGTGCTGGAATGTACCCCAATAGAGGTGGTTTCAGCGGGGATGGGAGCATGCTTTTAGGACCTAATGACCCGCGATGGTTTGGAATTGGTAGAGGACCTGGTTTTCAAGGCGGGCAGCCGGGCGTTCCTCCAGGTGCTCGTTTTGATCCATATGGTCCACCTGGTGTTCCTGGGTTTGAACCAAATCGGTTTGTTAGAAATCCTAGGAGACCAGGAGGAGGGACTCACCCGGACCTGGAGCATTTTGGAGGCGGTTCAGATTTTATATAG

Coding sequence (CDS)

ATGGTGAACGAGCAATCGGTAATGGCCGTCATCAGGGCAACTAGGGCTAGCTTTCGTAACGATTACGACAAGGTCGCGTTTGCAGTTCACGCCACATTTCTCGCCTCCGGCTTCGTGCTCACTGCCACCGGACCTTCTGCTTTTACTGAAGCCGCCTTTTCTTCTCCCTCTACGGATGAAGTTAGTATCGAGGGCTGGAACGAATTGGAGGATGAGTACGCTTACGTTTACGCAAACCCTGAAAGGGATTCTAATTGGAAGAAAATGATAGTGAAGTGCCTGGTGATGAACGGGAAATTGCTTATTGATGCCTTGACTGATAACAATTCGGACCCGCTGCATCTTGAAATTGACGTTGAAGAAATCGTCAATCCCAATGGGGGAAGTAGCTATACAACTCAGTACAGAAATTTGGATAAACTGGTGGAACGCTTGGACTTGGGAATATTGAGTAAATTGCATGGTTCTTCAAAAGCTACGAGAAGTAGTTCTACAAGGTCTGAGAGTAGTGAAAGATCATCTGCTGCTGTAAATGAACCTACTTCTGGAATCCCTGAGAATCATAGCCCCCCATCGAATATGCCTATGTTTGGAATTCCTGAGCGTCCTGGTCCACAAATCGATCCTTCAGGAGTTGTAATTCCACCTATTAATCCCTTTGGTGGTGGTGATCTTTTCCCTGGACCTGGTGCTGGAATGTACCCCAATAGAGGTGGTTTCAGCGGGGATGGGAGCATGCTTTTAGGACCTAATGACCCGCGATGGTTTGGAATTGGTAGAGGACCTGGTTTTCAAGGCGGGCAGCCGGGCGTTCCTCCAGGTGCTCGTTTTGATCCATATGGTCCACCTGGTGTTCCTGGGTTTGAACCAAATCGGTTTGTTAGAAATCCTAGGAGACCAGGAGGAGGGACTCACCCGGACCTGGAGCATTTTGGAGGCGGTTCAGATTTTATATAG

Protein sequence

MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDEVSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVEEIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEPTSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGFSGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRPGGGTHPDLEHFGGGSDFI
Homology
BLAST of Csor.00g041690 vs. ExPASy Swiss-Prot
Match: Q9M330 (Probable proteasome inhibitor OS=Arabidopsis thaliana OX=3702 GN=At3g53970 PE=1 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.3e-83
Identity = 179/324 (55.25%), Postives = 217/324 (66.98%), Query Frame = 0

Query: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60
           M N Q+VMA+IR  R  FRN++DKVAFA+H++F+ASG++LTATG  AF + A SS S ++
Sbjct: 1   MANSQTVMAMIRLARPPFRNNHDKVAFAIHSSFVASGYILTATGRPAFADEALSSSSQND 60

Query: 61  VSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVE 120
           V IEGWNE E EYA+VYANP++ S  KK++VKCL M+ KLL+DA+ D  ++P HLEI V 
Sbjct: 61  VGIEGWNEFEGEYAFVYANPKKGS--KKILVKCLAMDDKLLVDAIADGGAEPAHLEIKVG 120

Query: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180
           +    +    Y+ Q++NLDKLV  L   I+ KL G  K   S +       +SS+  NE 
Sbjct: 121 DYAEESNEGDYSAQFKNLDKLVTDLQSEIIDKLDGKPKPVASRA-------QSSSETNEE 180

Query: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGG-GDLFPGPGAGMYPNRGG 240
                +  +P             GPQI PSGVV+PPI   GG  DLFPGPGAGMYP RGG
Sbjct: 181 PRYYDDTPNP------------LGPQIHPSGVVVPPIPGNGGYSDLFPGPGAGMYPGRGG 240

Query: 241 FSGDGSMLLGPNDPRWFGIGRG---PGFQG-GQPGV-PPGARFDPYGPPGVPGFEPNRFV 300
           F GDGSML+GP DPR+F  G G   PGF G   PG+ PPGARFDPYGPPGVPGFEP RF 
Sbjct: 241 F-GDGSMLVGPTDPRFFPFGDGSDRPGFMGPPHPGMPPPGARFDPYGPPGVPGFEPGRFT 300

Query: 301 RNPRRPGGGTHPDLEHFGGGSDFI 319
           R P R  GG HPDLEHF GGSDFI
Sbjct: 301 RQPPRGPGGGHPDLEHFPGGSDFI 302

BLAST of Csor.00g041690 vs. ExPASy Swiss-Prot
Match: Q92530 (Proteasome inhibitor PI31 subunit OS=Homo sapiens OX=9606 GN=PSMF1 PE=1 SV=2)

HSP 1 Score: 51.6 bits (122), Expect = 1.9e-05
Identity = 62/229 (27.07%), Postives = 95/229 (41.48%), Query Frame = 0

Query: 65  GWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVEEIVN 124
           GWN  +D Y   Y   E     +K++VK + +   ++++ L   +     L +++++ ++
Sbjct: 57  GWNNNKDLYVLRY---EYKDGSRKLLVKAITVESSMILNVLEYGSQQVADLTLNLDDYID 116

Query: 125 PNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEPTSGI 184
                 +   Y+N ++L  R+  GI++ +H   +    SS   E    ++  V+     I
Sbjct: 117 AEHLGDFHRTYKNSEELRSRIVSGIITPIHEQWEKANVSSPHREFPPATAREVD--PLRI 176

Query: 185 PENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGFSGDG 244
           P +H   S  P         P  DP G  +      GG DL P       P RGG   D 
Sbjct: 177 PPHHPHTSRQP---------PWCDPLGPFV-----VGGEDLDP-----FGPRRGGMIVDP 236

Query: 245 SMLLGPNDPRWFGIGRGPGFQGGQP--GVPPGARFDPYGPPGVPGFEPN 292
              L    PR   I    G     P   VPPGARFDP+GP G     PN
Sbjct: 237 ---LRSGFPRAL-IDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPPGPN 257

BLAST of Csor.00g041690 vs. ExPASy Swiss-Prot
Match: Q5RDN3 (Proteasome inhibitor PI31 subunit OS=Pongo abelii OX=9601 GN=PSMF1 PE=2 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 6.1e-04
Identity = 63/231 (27.27%), Postives = 95/231 (41.13%), Query Frame = 0

Query: 65  GWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVEEIVN 124
           GWN  +D Y   Y   E     +K++VK + +   ++++ L   +     L +++++ ++
Sbjct: 57  GWNNNKDLYVLRY---EYKDGSRKLLVKAITVESSMILNVLEYGSQQVADLTLNLDDYID 116

Query: 125 PNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEPTSGI 184
                 +   Y+N ++L  R+  GI++ +H   +    SS   E    ++  V+     I
Sbjct: 117 AEHLGDFHRTYKNSEELRSRIVSGIITPIHEQWEKANVSSPHREFPPATAREVD--PLRI 176

Query: 185 PENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPF--GGGDLFPGPGAGMYPNRGGFSG 244
           P +H   S  P         P  DP G       PF  GG DL P         RGG   
Sbjct: 177 PPHHPHTSRQP---------PWCDPLG-------PFAVGGEDLDP-----FGHRRGGMIV 236

Query: 245 DGSMLLGPNDPRWFGIGRGPGFQGGQP--GVPPGARFDPYGPPGVPGFEPN 292
           D    L    PR   I    G     P   VPPGARFDP+GP G     PN
Sbjct: 237 DP---LRSGFPRAL-IDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPPGPN 257

BLAST of Csor.00g041690 vs. NCBI nr
Match: KAG6595354.1 (putative proteasome inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 637 bits (1644), Expect = 1.43e-230
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0

Query: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60
           MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE
Sbjct: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60

Query: 61  VSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVE 120
           VSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVE
Sbjct: 61  VSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVE 120

Query: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180
           EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP
Sbjct: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180

Query: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240
           TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF
Sbjct: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240

Query: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300
           SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP
Sbjct: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300

Query: 301 GGGTHPDLEHFGGGSDFI 318
           GGGTHPDLEHFGGGSDFI
Sbjct: 301 GGGTHPDLEHFGGGSDFI 318

BLAST of Csor.00g041690 vs. NCBI nr
Match: XP_022931882.1 (probable proteasome inhibitor [Cucurbita moschata])

HSP 1 Score: 634 bits (1635), Expect = 3.37e-229
Identity = 316/318 (99.37%), Postives = 317/318 (99.69%), Query Frame = 0

Query: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60
           MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE
Sbjct: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60

Query: 61  VSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVE 120
           VSIEGWNELEDEYAYVYANPE+DSNWKKMIVKCLVMNGKLLIDALTD NSDPLHLEIDVE
Sbjct: 61  VSIEGWNELEDEYAYVYANPEKDSNWKKMIVKCLVMNGKLLIDALTDKNSDPLHLEIDVE 120

Query: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180
           EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP
Sbjct: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180

Query: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240
           TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF
Sbjct: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240

Query: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300
           SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP
Sbjct: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300

Query: 301 GGGTHPDLEHFGGGSDFI 318
           GGGTHPDLEHFGGGSDFI
Sbjct: 301 GGGTHPDLEHFGGGSDFI 318

BLAST of Csor.00g041690 vs. NCBI nr
Match: XP_023517596.1 (probable proteasome inhibitor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 632 bits (1631), Expect = 1.37e-228
Identity = 315/318 (99.06%), Postives = 316/318 (99.37%), Query Frame = 0

Query: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60
           MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE
Sbjct: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60

Query: 61  VSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVE 120
           VSIEGWNELEDEYAYVYANPE+DSNWKKMIVKCLVMNGKLLIDALTD NSDPLHLEIDVE
Sbjct: 61  VSIEGWNELEDEYAYVYANPEKDSNWKKMIVKCLVMNGKLLIDALTDKNSDPLHLEIDVE 120

Query: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180
           EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSA VNEP
Sbjct: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAVVNEP 180

Query: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240
           TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF
Sbjct: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240

Query: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300
           SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP
Sbjct: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300

Query: 301 GGGTHPDLEHFGGGSDFI 318
           GGGTHPDLEHFGGGSDFI
Sbjct: 301 GGGTHPDLEHFGGGSDFI 318

BLAST of Csor.00g041690 vs. NCBI nr
Match: XP_022966596.1 (probable proteasome inhibitor [Cucurbita maxima])

HSP 1 Score: 627 bits (1616), Expect = 2.66e-226
Identity = 312/318 (98.11%), Postives = 315/318 (99.06%), Query Frame = 0

Query: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60
           MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE
Sbjct: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60

Query: 61  VSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVE 120
           VSIEGWNELEDEYAYVYANPE+DSNWKKMIVKCLVMNGKLLIDALTD NSDPLHLEIDVE
Sbjct: 61  VSIEGWNELEDEYAYVYANPEKDSNWKKMIVKCLVMNGKLLIDALTDKNSDPLHLEIDVE 120

Query: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180
           EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKL+GSSKATRSSSTRSESSERS A +NEP
Sbjct: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLYGSSKATRSSSTRSESSERSFAVLNEP 180

Query: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240
           TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF
Sbjct: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240

Query: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300
           SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP
Sbjct: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300

Query: 301 GGGTHPDLEHFGGGSDFI 318
           GGGTHPDLEHFGGGSDFI
Sbjct: 301 GGGTHPDLEHFGGGSDFI 318

BLAST of Csor.00g041690 vs. NCBI nr
Match: XP_038883482.1 (probable proteasome inhibitor [Benincasa hispida])

HSP 1 Score: 582 bits (1501), Expect = 8.97e-209
Identity = 289/318 (90.88%), Postives = 303/318 (95.28%), Query Frame = 0

Query: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60
           MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE
Sbjct: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60

Query: 61  VSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVE 120
           VSIEGWNELEDEYAY+YAN E+D+NWKK+IVKCLVMNGKLLIDAL D NS+PLHLEIDVE
Sbjct: 61  VSIEGWNELEDEYAYIYANLEKDANWKKVIVKCLVMNGKLLIDALIDKNSEPLHLEIDVE 120

Query: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180
           EIVNPNGGS+Y+TQYRNLDKLV+RLDL ILSKL GSSKAT S+  R+ESSERSSA VNEP
Sbjct: 121 EIVNPNGGSNYSTQYRNLDKLVKRLDLEILSKLDGSSKATTSNPARAESSERSSAVVNEP 180

Query: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240
            SGIPE  SPPSNMP++GIP+RPGP IDPSGVVIPPINPFGGGDLFPGPGAGMYP RGGF
Sbjct: 181 ASGIPETRSPPSNMPLYGIPQRPGPLIDPSGVVIPPINPFGGGDLFPGPGAGMYPARGGF 240

Query: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300
           SGDGSML+GPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPP VPGFEPNRFVRNPRRP
Sbjct: 241 SGDGSMLIGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPDVPGFEPNRFVRNPRRP 300

Query: 301 GGGTHPDLEHFGGGSDFI 318
           GGGTHPDLEHFGGGSDFI
Sbjct: 301 GGGTHPDLEHFGGGSDFI 318

BLAST of Csor.00g041690 vs. ExPASy TrEMBL
Match: A0A6J1EUZ4 (probable proteasome inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111438164 PE=4 SV=1)

HSP 1 Score: 634 bits (1635), Expect = 1.63e-229
Identity = 316/318 (99.37%), Postives = 317/318 (99.69%), Query Frame = 0

Query: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60
           MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE
Sbjct: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60

Query: 61  VSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVE 120
           VSIEGWNELEDEYAYVYANPE+DSNWKKMIVKCLVMNGKLLIDALTD NSDPLHLEIDVE
Sbjct: 61  VSIEGWNELEDEYAYVYANPEKDSNWKKMIVKCLVMNGKLLIDALTDKNSDPLHLEIDVE 120

Query: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180
           EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP
Sbjct: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180

Query: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240
           TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF
Sbjct: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240

Query: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300
           SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP
Sbjct: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300

Query: 301 GGGTHPDLEHFGGGSDFI 318
           GGGTHPDLEHFGGGSDFI
Sbjct: 301 GGGTHPDLEHFGGGSDFI 318

BLAST of Csor.00g041690 vs. ExPASy TrEMBL
Match: A0A6J1HPR8 (probable proteasome inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111466228 PE=4 SV=1)

HSP 1 Score: 627 bits (1616), Expect = 1.29e-226
Identity = 312/318 (98.11%), Postives = 315/318 (99.06%), Query Frame = 0

Query: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60
           MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE
Sbjct: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60

Query: 61  VSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVE 120
           VSIEGWNELEDEYAYVYANPE+DSNWKKMIVKCLVMNGKLLIDALTD NSDPLHLEIDVE
Sbjct: 61  VSIEGWNELEDEYAYVYANPEKDSNWKKMIVKCLVMNGKLLIDALTDKNSDPLHLEIDVE 120

Query: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180
           EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKL+GSSKATRSSSTRSESSERS A +NEP
Sbjct: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLYGSSKATRSSSTRSESSERSFAVLNEP 180

Query: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240
           TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF
Sbjct: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240

Query: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300
           SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP
Sbjct: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300

Query: 301 GGGTHPDLEHFGGGSDFI 318
           GGGTHPDLEHFGGGSDFI
Sbjct: 301 GGGTHPDLEHFGGGSDFI 318

BLAST of Csor.00g041690 vs. ExPASy TrEMBL
Match: A0A5A7TPR9 (Putative proteasome inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G001250 PE=4 SV=1)

HSP 1 Score: 581 bits (1497), Expect = 1.77e-208
Identity = 288/318 (90.57%), Postives = 301/318 (94.65%), Query Frame = 0

Query: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60
           MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE
Sbjct: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60

Query: 61  VSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVE 120
           VSIEGWN+LEDEYAY+YAN E+DSNWKK+IVKCLVMNGKLL+D LTD NS+PLHLEIDVE
Sbjct: 61  VSIEGWNDLEDEYAYIYANLEKDSNWKKVIVKCLVMNGKLLVDVLTDKNSEPLHLEIDVE 120

Query: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180
           EIVNPNGGS+YTTQYRNLDKLV+RLDL ILSKL GSSKA  S+  R+ESSERSS  VNEP
Sbjct: 121 EIVNPNGGSNYTTQYRNLDKLVKRLDLEILSKLDGSSKALTSNPGRAESSERSSGVVNEP 180

Query: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240
           +SGIPE HSPPSNMP++GIPERPGP I PSGVVIPPINP GGGDLFPGPGAGMYP RGGF
Sbjct: 181 SSGIPETHSPPSNMPLYGIPERPGPMIHPSGVVIPPINPLGGGDLFPGPGAGMYPARGGF 240

Query: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300
           SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPP VPGFEPNRFVRNPRRP
Sbjct: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPDVPGFEPNRFVRNPRRP 300

Query: 301 GGGTHPDLEHFGGGSDFI 318
           GGGTHPDLEHFGGGSDFI
Sbjct: 301 GGGTHPDLEHFGGGSDFI 318

BLAST of Csor.00g041690 vs. ExPASy TrEMBL
Match: A0A1S3BJY5 (probable proteasome inhibitor OS=Cucumis melo OX=3656 GN=LOC103490685 PE=4 SV=1)

HSP 1 Score: 581 bits (1497), Expect = 1.77e-208
Identity = 288/318 (90.57%), Postives = 301/318 (94.65%), Query Frame = 0

Query: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60
           MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE
Sbjct: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60

Query: 61  VSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVE 120
           VSIEGWN+LEDEYAY+YAN E+DSNWKK+IVKCLVMNGKLL+D LTD NS+PLHLEIDVE
Sbjct: 61  VSIEGWNDLEDEYAYIYANLEKDSNWKKVIVKCLVMNGKLLVDVLTDKNSEPLHLEIDVE 120

Query: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180
           EIVNPNGGS+YTTQYRNLDKLV+RLDL ILSKL GSSKA  S+  R+ESSERSS  VNEP
Sbjct: 121 EIVNPNGGSNYTTQYRNLDKLVKRLDLEILSKLDGSSKALTSNPGRAESSERSSGVVNEP 180

Query: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240
           +SGIPE HSPPSNMP++GIPERPGP I PSGVVIPPINP GGGDLFPGPGAGMYP RGGF
Sbjct: 181 SSGIPETHSPPSNMPLYGIPERPGPMIHPSGVVIPPINPLGGGDLFPGPGAGMYPARGGF 240

Query: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300
           SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPP VPGFEPNRFVRNPRRP
Sbjct: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPDVPGFEPNRFVRNPRRP 300

Query: 301 GGGTHPDLEHFGGGSDFI 318
           GGGTHPDLEHFGGGSDFI
Sbjct: 301 GGGTHPDLEHFGGGSDFI 318

BLAST of Csor.00g041690 vs. ExPASy TrEMBL
Match: A0A0A0KZR4 (PI31_Prot_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G303080 PE=4 SV=1)

HSP 1 Score: 576 bits (1485), Expect = 1.19e-206
Identity = 285/318 (89.62%), Postives = 300/318 (94.34%), Query Frame = 0

Query: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60
           MVNEQSVMAVIRATRA+FRNDYDKVAFAVHATFLASGFVLTATGPSAFTE  FSSPSTDE
Sbjct: 1   MVNEQSVMAVIRATRATFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEPTFSSPSTDE 60

Query: 61  VSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVE 120
           VSIEGWN+LEDEYAY+YAN E+DSNWKK+IVKCLVMNGKLL+DALTD NS+PLHLEIDVE
Sbjct: 61  VSIEGWNDLEDEYAYIYANLEKDSNWKKVIVKCLVMNGKLLVDALTDKNSEPLHLEIDVE 120

Query: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180
           EIVNPNGGS+YTTQYRNLDKLV+RLDL ILSKL GSSKA+ S+  R+ESSERSS  VNEP
Sbjct: 121 EIVNPNGGSNYTTQYRNLDKLVKRLDLEILSKLDGSSKASTSNPARAESSERSSGVVNEP 180

Query: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGGGDLFPGPGAGMYPNRGGF 240
           +SGIPE  SPPSNMP++GIPERPGP I PSGVVIPPINP GGGDLFPGPGAGMYP RGGF
Sbjct: 181 SSGIPETRSPPSNMPLYGIPERPGPMIHPSGVVIPPINPLGGGDLFPGPGAGMYPARGGF 240

Query: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPGVPGFEPNRFVRNPRRP 300
           SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPP VPGFEPNRFVRNPRRP
Sbjct: 241 SGDGSMLLGPNDPRWFGIGRGPGFQGGQPGVPPGARFDPYGPPDVPGFEPNRFVRNPRRP 300

Query: 301 GGGTHPDLEHFGGGSDFI 318
           GGGTHPDLEHFGGGSDFI
Sbjct: 301 GGGTHPDLEHFGGGSDFI 318

BLAST of Csor.00g041690 vs. TAIR 10
Match: AT3G53970.1 (proteasome inhibitor-related )

HSP 1 Score: 311.2 bits (796), Expect = 9.4e-85
Identity = 179/324 (55.25%), Postives = 217/324 (66.98%), Query Frame = 0

Query: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60
           M N Q+VMA+IR  R  FRN++DKVAFA+H++F+ASG++LTATG  AF + A SS S ++
Sbjct: 1   MANSQTVMAMIRLARPPFRNNHDKVAFAIHSSFVASGYILTATGRPAFADEALSSSSQND 60

Query: 61  VSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVE 120
           V IEGWNE E EYA+VYANP++ S  KK++VKCL M+ KLL+DA+ D  ++P HLEI V 
Sbjct: 61  VGIEGWNEFEGEYAFVYANPKKGS--KKILVKCLAMDDKLLVDAIADGGAEPAHLEIKVG 120

Query: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180
           +    +    Y+ Q++NLDKLV  L   I+ KL G  K   S +       +SS+  NE 
Sbjct: 121 DYAEESNEGDYSAQFKNLDKLVTDLQSEIIDKLDGKPKPVASRA-------QSSSETNEE 180

Query: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGG-GDLFPGPGAGMYPNRGG 240
                +  +P             GPQI PSGVV+PPI   GG  DLFPGPGAGMYP RGG
Sbjct: 181 PRYYDDTPNP------------LGPQIHPSGVVVPPIPGNGGYSDLFPGPGAGMYPGRGG 240

Query: 241 FSGDGSMLLGPNDPRWFGIGRG---PGFQG-GQPGV-PPGARFDPYGPPGVPGFEPNRFV 300
           F GDGSML+GP DPR+F  G G   PGF G   PG+ PPGARFDPYGPPGVPGFEP RF 
Sbjct: 241 F-GDGSMLVGPTDPRFFPFGDGSDRPGFMGPPHPGMPPPGARFDPYGPPGVPGFEPGRFT 300

Query: 301 RNPRRPGGGTHPDLEHFGGGSDFI 319
           R P R  GG HPDLEHF GGSDFI
Sbjct: 301 RQPPRGPGGGHPDLEHFPGGSDFI 302

BLAST of Csor.00g041690 vs. TAIR 10
Match: AT3G53970.2 (proteasome inhibitor-related )

HSP 1 Score: 235.7 bits (600), Expect = 5.0e-62
Identity = 138/270 (51.11%), Postives = 175/270 (64.81%), Query Frame = 0

Query: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTDE 60
           M N Q+VMA+IR  R  FRN++DKVAFA+H++F+ASG++LTATG  AF + A SS S ++
Sbjct: 1   MANSQTVMAMIRLARPPFRNNHDKVAFAIHSSFVASGYILTATGRPAFADEALSSSSQND 60

Query: 61  VSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDVE 120
           V IEGWNE E EYA+VYANP++ S  KK++VKCL M+ KLL+DA+ D  ++P HLEI V 
Sbjct: 61  VGIEGWNEFEGEYAFVYANPKKGS--KKILVKCLAMDDKLLVDAIADGGAEPAHLEIKVG 120

Query: 121 EIVNPNGGSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSSTRSESSERSSAAVNEP 180
           +    +    Y+ Q++NLDKLV  L   I+ KL G  K   S +       +SS+  NE 
Sbjct: 121 DYAEESNEGDYSAQFKNLDKLVTDLQSEIIDKLDGKPKPVASRA-------QSSSETNEE 180

Query: 181 TSGIPENHSPPSNMPMFGIPERPGPQIDPSGVVIPPINPFGG-GDLFPGPGAGMYPNRGG 240
                +  +P             GPQI PSGVV+PPI   GG  DLFPGPGAGMYP RGG
Sbjct: 181 PRYYDDTPNP------------LGPQIHPSGVVVPPIPGNGGYSDLFPGPGAGMYPGRGG 240

Query: 241 FSGDGSMLLGPNDPRWFGIGRG---PGFQG 267
           F GDGSML+GP DPR+F  G G   PGF G
Sbjct: 241 F-GDGSMLVGPTDPRFFPFGDGSDRPGFMG 248

BLAST of Csor.00g041690 vs. TAIR 10
Match: AT1G48530.1 (CONTAINS InterPro DOMAIN/s: Proteasome Inhibitor PI31 (InterPro:IPR021625); BEST Arabidopsis thaliana protein match is: proteasome inhibitor-related (TAIR:AT3G53970.2); Has 43 Blast hits to 43 proteins in 18 species: Archae - 0; Bacteria - 0; Metazoa - 11; Fungi - 0; Plants - 32; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 133.3 bits (334), Expect = 3.5e-31
Identity = 77/166 (46.39%), Postives = 104/166 (62.65%), Query Frame = 0

Query: 1   MVNEQSVMAVIRATRASFRNDYDKVAFAVHATFLASGFVLTATGPSAFTEAAFSSPSTD- 60
           M    +VM VIR+   +FRN  DK+AF VHA+   SGF+LT+TG  AF   A SS +T  
Sbjct: 12  MATTHAVMGVIRSVMPAFRNKNDKIAFVVHASLAVSGFILTSTGRPAFAHDALSSSTTQC 71

Query: 61  EVSIEGWNELEDEYAYVYANPERDSNWKKMIVKCLVMNGKLLIDALTDNNSDPLHLEIDV 120
            V IEGWNE ++EYA+VY  P      K+ +VKCL MN KLL+DA+ ++  +  HL+I+V
Sbjct: 72  LVGIEGWNEFDEEYAFVYKCPV-----KRYLVKCLAMNDKLLVDAIAEDGKEFGHLQIEV 131

Query: 121 EEIVNPNG-GSSYTTQYRNLDKLVERLDLGILSKLHGSSKATRSSS 165
              ++ +G    Y TQ++N DKLV  L   IL KL+  S  T+SSS
Sbjct: 132 GNYIDESGEEGDYDTQFKNFDKLVTELKSEILCKLNTVSTTTKSSS 172

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M3301.3e-8355.25Probable proteasome inhibitor OS=Arabidopsis thaliana OX=3702 GN=At3g53970 PE=1 ... [more]
Q925301.9e-0527.07Proteasome inhibitor PI31 subunit OS=Homo sapiens OX=9606 GN=PSMF1 PE=1 SV=2[more]
Q5RDN36.1e-0427.27Proteasome inhibitor PI31 subunit OS=Pongo abelii OX=9601 GN=PSMF1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6595354.11.43e-230100.00putative proteasome inhibitor, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022931882.13.37e-22999.37probable proteasome inhibitor [Cucurbita moschata][more]
XP_023517596.11.37e-22899.06probable proteasome inhibitor [Cucurbita pepo subsp. pepo][more]
XP_022966596.12.66e-22698.11probable proteasome inhibitor [Cucurbita maxima][more]
XP_038883482.18.97e-20990.88probable proteasome inhibitor [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1EUZ41.63e-22999.37probable proteasome inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111438164 PE=4... [more]
A0A6J1HPR81.29e-22698.11probable proteasome inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111466228 PE=4 S... [more]
A0A5A7TPR91.77e-20890.57Putative proteasome inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3BJY51.77e-20890.57probable proteasome inhibitor OS=Cucumis melo OX=3656 GN=LOC103490685 PE=4 SV=1[more]
A0A0A0KZR41.19e-20689.62PI31_Prot_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G303080... [more]
Match NameE-valueIdentityDescription
AT3G53970.19.4e-8555.25proteasome inhibitor-related [more]
AT3G53970.25.0e-6251.11proteasome inhibitor-related [more]
AT1G48530.13.5e-3146.39CONTAINS InterPro DOMAIN/s: Proteasome Inhibitor PI31 (InterPro:IPR021625); BEST... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021625PI31 proteasome regulator, N-terminalPFAMPF11566PI31_Prot_Ncoord: 15..156
e-value: 9.2E-18
score: 64.5
NoneNo IPR availableGENE3D3.40.1000.30coord: 2..169
e-value: 7.0E-42
score: 144.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 156..210
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 156..189
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 283..318
IPR045128Proteasome inhibitor PI31-likePANTHERPTHR13266PROTEASOME INHIBITORcoord: 1..314

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g041690.m01Csor.00g041690.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
molecular_function GO:0004866 endopeptidase inhibitor activity
molecular_function GO:0070628 proteasome binding