Csor.00g036830 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g036830
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionmeiotic recombination protein SPO11-1
LocationCsor_Chr09: 5348360 .. 5351279 (+)
RNA-Seq ExpressionCsor.00g036830
SyntenyCsor.00g036830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGGAAAGCTTGGAAGCTCGGAGAGAATCGACATCATGAGGAAAATCAGAGGTCACAATTCGATTGAATTTACTCATTCTCATATTCATTCCATACGGTTTCTGTTTCAACGTTGCTATTTTCTTTTTCGCTTTTCGATTCCATGTTTTGCAGAGCTAACAAGATCTATGATCGTTGAACTGAGTAATGGAAGCTCCAAGATGATCGCTATCGATCGGTTTAAAAATTACTGCACTAATTCCACTGAAAACTGGTTCGGATTTTGTTTTGCTTGTTTGCAGTTTTTGTTACTTTTGAATCTTCATAGTCGTACCAAATAATGATCACGATCATCATGTTTCTATGTGATTTACTTGACTACGATTATTTAGTTCTCAATACGTATGACAATTTTCGATAATCTTCGATTTGTTATTTACCAATGGATCTGTTGGATCCTATCGGAGGCCGAGTCAATCGGTTGCATAACTTAGCCGCGATTTGTCATTTTCGAAAGGCACTTCTCTGTCTAAACTCATAAGCATTTAATATGTAGGACCTAGTTCTCACTTATCCTTTTCGTAATAAACGAATGGTGTTCCAAACAGTAGTTTAATCTTTCAACAAAATACAGTTCGCGAATGTGCTGTGGCCACTATCTTGAAATTGGACATGTGCTTTGGAAATGTATGTTGTCTACCTAAAGAGATGTTTAGACGATTATGGCTATTTTTTATACAGTTGCTGCAGGTTCAATTTATCTGAAGGGAAGGAGATTCTAACACTTCAAAAGGAAAGCCATGTCCGTAGACTAGGTGATTTAGTTTTGTCGACGAATGATATTATAAAATCATAATTCATATAGACTACAGAGTACACTTGTGACTTATTTGATATCATTTTGCTCTATCAGATGTCTTGCTTAGAGTTCTACTCATTATTCAGCAGCTTCTGCGAGAAAATAGACATAGCTCAAAGAGAGATATATATTATATGCATCCCTCTGTCTTTACAGGTAAAGCTAACCACCGTTTAATCCGCAGGCACCGGTAGATTTGTTTAAGATACATGTTTCATAAAATAATTAGCTCTCCAGTTGAGAAGAATTAAATTTCAACATATCCTTCGAATTTATGTTTTCAGCAAATTTGTGGCAGGAGATTTTCTGAACATCTCCTTGTTTTGCTTGATATGACAGAGTTTAATGACATTGGCTTCATTGTAATGATGAATATTTTGCAGATCAGTCTGTTGTTGACCGGGCAATCAACGACATCTGCATTCTTTTGCAATGTAGCCGGCACAATCTAAATGTGGTTGGTGCTGCGCTTCATTTTACTTTCTTTCCAATTTATGTTTCCTCCATAGTTATATAATACAGTTCATGTGGGATCTAATCTCAATATGATATTTACAGTCGGCTGCGTGTCACACAAAATGAGGTTCTTTGATAAAATAAACGTATAGTCATTTTTTGTGATCCAACAGCACTAATAGGTGTTGAAATTAGGAAGATACTCTTCAACAGATCAATTGCAGTTTTCTTTAAATCCTATAAACATGTTTGCAAGCTGCTTCTGATGATCATTTCAACTTGTGTCCTTTTGACAGGTTTCTGTTGGAAATGGGTAAGTATCGAGGAAAACATCTGCTCAGTTCAGTAAAGAAAAGACTAGTGCAGAGTGCTGCTTGCGTTCTTGGCATAAGCCTTTACAAGCAGTCTCCTTACACAATTATTTGTTTTGTGGGTCGATACCCATATCAGCCTTTTCTATACCATTAGATTTTGTTACATAACAATTTCCTCTTTTTGCTGCAATCTGTTTCATGGAGGAAGAAAATAAAGAACTGAATGTTGACGTAGTCACTTCTTTTACAGGTTGGTGATGGGTTGGTTGAGGTTCGCAGAAGCTGGTAGGAGATTTGATTGCATAAAGAAGCCTAACAATGTAAAAGTTATACTACGGTTCTTCATAATGCAATTGCCAATTTTATCACTCTTAGCTGTAACTGATTTGAACATATGCATCTCAATAACATACAGGCCCACTCCATTCCTGTTCAAGTTGAAGAAGTCACAGGTATTGCTGATACTGGATACATAACTTGCCAATCTGTTAGGCTAGAGCTTAAATTGGACACTCGGAATGATACCTTTGGATATGGATTGTCTATACTGTAGTCTGCCAAAGTTCTACATTTCTTAGTGAAATTATATAGTTCTTATGGTTGAGAATTGTTGAGAGGGAGTCCCACGTCTGCTAATTTAGGGAATATCATGAGTTTATAAGTAAGGAATACATCTCCATTGGTATGAGGCCAGTGATTCCTAACACTTATCGCATTCCTCCATATTCTTGAAAGCCCTTTTCACCCGATTCGGTTTTCGCTTATGACTATTTTTATCTTTATATGCAGATATTGCTAGTCCTGCGAATTACATACTCGTTGTTGAGAAAGAATCAGGTGAAGGCTATACTGATAAAAGCTGATTTCACGGTTCAAGGAATGAGTTTCACTTTGATCAGTTAAAAGTACCTGGTTCGATTTTTGAACGTGCAGTTTTTCAGCGTCTAGCAAATGACCATTTCTGCGGCAGAAACCGTTGCATTGTAATCTCGGTGAGCATGTCTCAGGTCCTGCAATTGAAAATTCTAATATTCAAATGTTAACATTTTCTTTTTTCAGGGACGTGGATATCCAGATGTTCCTACTAGAAGGTAAAGAAATAAATTATGGTCATAAAAACGCTAATCTCATTTTATTATTGATGATGCGAGACATGAAAATATGCACGCATGTATTGCTATCAGGTTTCTGCGGCTTCTCGTTGATGTCTTGGCATTGCCTGCATTTTGTTTGGTAGATTGTGATCCATATGGGTTTGACATTCTGACAACATATCGATTCGGTTCTATGGTTAGGCTCCTCAATTGA

mRNA sequence

ATGGCGGGAAAGCTTGGAAGCTCGGAGAGAATCGACATCATGAGGAAAATCAGAGAGCTAACAAGATCTATGATCGTTGAACTGAGTAATGGAAGCTCCAAGATGATCGCTATCGATCGGTTTAAAAATTACTGCACTAATTCCACTGAAAACTGGTTCAATTTATCTGAAGGGAAGGAGATTCTAACACTTCAAAAGGAAAGCCATGTCCGTAGACTAGATGTCTTGCTTAGAGTTCTACTCATTATTCAGCAGCTTCTGCGAGAAAATAGACATAGCTCAAAGAGAGATATATATTATATGCATCCCTCTGTCTTTACAGATCAGTCTGTTGTTGACCGGGCAATCAACGACATCTGCATTCTTTTGCAATGTAGCCGGCACAATCTAAATGTGGTTTCTGTTGGAAATGGGTTGGTGATGGGTTGGTTGAGGTTCGCAGAAGCTGGTAGGAGATTTGATTGCATAAAGAAGCCTAACAATGCCCACTCCATTCCTGTTCAAGTTGAAGAAGTCACAGATATTGCTAGTCCTGCGAATTACATACTCGTTGTTGAGAAAGAATCAGTTTTTCAGCGTCTAGCAAATGACCATTTCTGCGGCAGAAACCGTTGCATTGTAATCTCGGGACGTGGATATCCAGATGTTCCTACTAGAAGGTTTCTGCGGCTTCTCGTTGATGTCTTGGCATTGCCTGCATTTTGTTTGGTAGATTGTGATCCATATGGGTTTGACATTCTGACAACATATCGATTCGGTTCTATGGTTAGGCTCCTCAATTGA

Coding sequence (CDS)

ATGGCGGGAAAGCTTGGAAGCTCGGAGAGAATCGACATCATGAGGAAAATCAGAGAGCTAACAAGATCTATGATCGTTGAACTGAGTAATGGAAGCTCCAAGATGATCGCTATCGATCGGTTTAAAAATTACTGCACTAATTCCACTGAAAACTGGTTCAATTTATCTGAAGGGAAGGAGATTCTAACACTTCAAAAGGAAAGCCATGTCCGTAGACTAGATGTCTTGCTTAGAGTTCTACTCATTATTCAGCAGCTTCTGCGAGAAAATAGACATAGCTCAAAGAGAGATATATATTATATGCATCCCTCTGTCTTTACAGATCAGTCTGTTGTTGACCGGGCAATCAACGACATCTGCATTCTTTTGCAATGTAGCCGGCACAATCTAAATGTGGTTTCTGTTGGAAATGGGTTGGTGATGGGTTGGTTGAGGTTCGCAGAAGCTGGTAGGAGATTTGATTGCATAAAGAAGCCTAACAATGCCCACTCCATTCCTGTTCAAGTTGAAGAAGTCACAGATATTGCTAGTCCTGCGAATTACATACTCGTTGTTGAGAAAGAATCAGTTTTTCAGCGTCTAGCAAATGACCATTTCTGCGGCAGAAACCGTTGCATTGTAATCTCGGGACGTGGATATCCAGATGTTCCTACTAGAAGGTTTCTGCGGCTTCTCGTTGATGTCTTGGCATTGCCTGCATTTTGTTTGGTAGATTGTGATCCATATGGGTTTGACATTCTGACAACATATCGATTCGGTTCTATGGTTAGGCTCCTCAATTGA

Protein sequence

MAGKLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENWFNLSEGKEILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAINDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIASPANYILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLVDCDPYGFDILTTYRFGSMVRLLN
Homology
BLAST of Csor.00g036830 vs. ExPASy Swiss-Prot
Match: Q9M4A2 (Meiotic recombination protein SPO11-1 OS=Arabidopsis thaliana OX=3702 GN=SPO11-1 PE=1 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 1.4e-94
Identity = 169/258 (65.50%), Postives = 206/258 (79.84%), Query Frame = 0

Query: 1   MAGKLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENWFNLSE--- 60
           M GK   SE  +++++I++ T+S++V+L+ G S  I+I++F+NYC N   +    S+   
Sbjct: 1   MEGKFAISESTNLLQRIKDFTQSVVVDLAEGRSPKISINQFRNYCMNPEADCLCSSDKPK 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           G+EI TL+KE    R+D+LLRVLLI+QQLL+ENRH+SKRDIYYMHPS F  QS+VDRAI 
Sbjct: 61  GQEIFTLKKEPQTYRIDMLLRVLLIVQQLLQENRHASKRDIYYMHPSAFKAQSIVDRAIG 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIAS 180
           DICIL QCSR+NLNVVSVGNGLVMGWL+F EAGR+FDC+   N A+ +PV VEEV DI S
Sbjct: 121 DICILFQCSRYNLNVVSVGNGLVMGWLKFREAGRKFDCLNSLNTAYPVPVLVEEVEDIVS 180

Query: 181 PANYILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
            A YILVVEKE+VFQRLAND FC  NRCIVI+GRGYPDV TRRFLRLL++ L LP  CLV
Sbjct: 181 LAEYILVVEKETVFQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKLHLPVHCLV 240

Query: 241 DCDPYGFDILTTYRFGSM 256
           DCDPYGF+IL TYRFGSM
Sbjct: 241 DCDPYGFEILATYRFGSM 258

BLAST of Csor.00g036830 vs. ExPASy Swiss-Prot
Match: Q5ZPV8 (Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. indica OX=39946 GN=SPO11-1 PE=2 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.0e-82
Identity = 145/255 (56.86%), Postives = 194/255 (76.08%), Query Frame = 0

Query: 9   ERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTEN-------WFNLSEGKEI 68
           E   ++ +IR L R ++ E++ G S  +A+ R++NYC++++          +++  G ++
Sbjct: 24  EAATLLHRIRGLVRWVVAEVAAGRSPTVALHRYQNYCSSASAAAASPCACSYDVPVGTDV 83

Query: 69  LT-LQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAINDIC 128
           L+ L + SH  RL+VLLRVLL++QQLL++N+H SKRDIYYM+PS+F +Q+VVDRAINDIC
Sbjct: 84  LSLLHRGSHASRLNVLLRVLLVVQQLLQQNKHCSKRDIYYMYPSIFQEQAVVDRAINDIC 143

Query: 129 ILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIASPAN 188
           +L +CSRHNLNVV V  GLVMGW+RF E  +   C+   N A SIPV +E + D+ S A+
Sbjct: 144 VLFKCSRHNLNVVPVAKGLVMGWIRFLEGEKEVYCVTNVNAAFSIPVSIEAIKDVVSVAD 203

Query: 189 YILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLVDCD 248
           YIL+VEKE+VFQRLAND FC RNRCIVI+GRGYPD+PTRRFLR LV+ L LP +CLVD D
Sbjct: 204 YILIVEKETVFQRLANDKFCERNRCIVITGRGYPDIPTRRFLRYLVEQLHLPVYCLVDAD 263

Query: 249 PYGFDILTTYRFGSM 256
           PYGFDIL TY+FGS+
Sbjct: 264 PYGFDILATYKFGSL 278

BLAST of Csor.00g036830 vs. ExPASy Swiss-Prot
Match: Q7Y021 (Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. japonica OX=39947 GN=SPO11-1 PE=2 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.0e-82
Identity = 145/255 (56.86%), Postives = 194/255 (76.08%), Query Frame = 0

Query: 9   ERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTEN-------WFNLSEGKEI 68
           E   ++ +IR L R ++ E++ G S  +A+ R++NYC++++          +++  G ++
Sbjct: 24  EAATLLHRIRGLVRWVVAEVAAGRSPTVALHRYQNYCSSASAAAASPCACSYDVPVGTDV 83

Query: 69  LT-LQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAINDIC 128
           L+ L + SH  RL+VLLRVLL++QQLL++N+H SKRDIYYM+PS+F +Q+VVDRAINDIC
Sbjct: 84  LSLLHRGSHASRLNVLLRVLLVVQQLLQQNKHCSKRDIYYMYPSIFQEQAVVDRAINDIC 143

Query: 129 ILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIASPAN 188
           +L +CSRHNLNVV V  GLVMGW+RF E  +   C+   N A SIPV +E + D+ S A+
Sbjct: 144 VLFKCSRHNLNVVPVAKGLVMGWIRFLEGEKEVYCVTNVNAAFSIPVSIEAIKDVVSVAD 203

Query: 189 YILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLVDCD 248
           YIL+VEKE+VFQRLAND FC RNRCIVI+GRGYPD+PTRRFLR LV+ L LP +CLVD D
Sbjct: 204 YILIVEKETVFQRLANDKFCERNRCIVITGRGYPDIPTRRFLRYLVEQLHLPVYCLVDAD 263

Query: 249 PYGFDILTTYRFGSM 256
           PYGFDIL TY+FGS+
Sbjct: 264 PYGFDILATYKFGSL 278

BLAST of Csor.00g036830 vs. ExPASy Swiss-Prot
Match: Q9LZ03 (DNA topoisomerase 6 subunit A OS=Arabidopsis thaliana OX=3702 GN=TOP6A PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.5e-37
Identity = 77/187 (41.18%), Postives = 113/187 (60.43%), Query Frame = 0

Query: 68  SHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAINDICILLQCSR 127
           S VR+  +  R+L +I QL   N H +KRD++Y    +F DQ+  D  ++D+  +L C+R
Sbjct: 138 SSVRKTTITTRILALIHQLCLRNIHVTKRDLFYTDVKLFQDQTQSDAVLDDVSCMLGCTR 197

Query: 128 HNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIASPANYILVVEK 187
            +LNV++   G+V+G L F++ G   DC K      +IP  ++ V D+ S A +IL+VEK
Sbjct: 198 SSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQSDAMFILLVEK 257

Query: 188 ESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLVDCDPYGFDIL 247
           ++ + RLA D F  R  CI+++ +G PDV TR FLR +   L LP   LVD DPYG  IL
Sbjct: 258 DAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALVDSDPYGLKIL 317

Query: 248 TTYRFGS 255
           + Y  GS
Sbjct: 318 SVYGCGS 324

BLAST of Csor.00g036830 vs. ExPASy Swiss-Prot
Match: Q9Y5K1 (Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 5.8e-37
Identity = 83/258 (32.17%), Postives = 152/258 (58.91%), Query Frame = 0

Query: 4   KLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENWFNLSEGKEILT 63
           +L SS   +++  I  + + +I  L+   +    ID   ++     E+   L       T
Sbjct: 40  RLASSS--EVLASIENIIQDIITSLARNEAPAFTIDNRSSWENIKFEDSVGLQMVSHCTT 99

Query: 64  LQKES----HVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAINDI 123
            + +S      ++  ++L++L +I +L++ N +++KRDIYY    +F +Q+VVD  INDI
Sbjct: 100 RKIKSDSPKSAQKFSLILKILSMIYKLVQSNTYATKRDIYYTDSQLFGNQTVVDNIINDI 159

Query: 124 CILLQCSRHNLNVVSVGNGLVMGWLRFAEA-GRRFDCIKKPNNAHSIPVQVEEVTDIASP 183
             +L+ SR +L+++S   GL+ G LR+ E  G + +C      A ++P  ++ + ++ + 
Sbjct: 160 SCMLKVSRRSLHILSTSKGLIAGNLRYIEEDGTKVNCTCGA-TAVAVPSNIQGIRNLVTD 219

Query: 184 ANYILVVEKESVFQRLANDHFCGR-NRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 243
           A ++L+VEK++ FQRL +D+FC + + CI+I+G+G PD+ TR  ++ L D   +P F LV
Sbjct: 220 AKFVLIVEKDATFQRLLDDNFCNKLSPCIMITGKGVPDLNTRLLVKKLWDTFHVPVFTLV 279

Query: 244 DCDPYGFDILTTYRFGSM 256
           D DP+G +I+  Y++GSM
Sbjct: 280 DADPHGIEIMCIYKYGSM 294

BLAST of Csor.00g036830 vs. NCBI nr
Match: KAG6592090.1 (Meiotic recombination protein SPO11-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 522 bits (1345), Expect = 6.08e-187
Identity = 260/260 (100.00%), Postives = 260/260 (100.00%), Query Frame = 0

Query: 1   MAGKLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENWFNLSEGKE 60
           MAGKLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENWFNLSEGKE
Sbjct: 1   MAGKLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENWFNLSEGKE 60

Query: 61  ILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAINDIC 120
           ILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAINDIC
Sbjct: 61  ILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAINDIC 120

Query: 121 ILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIASPAN 180
           ILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIASPAN
Sbjct: 121 ILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIASPAN 180

Query: 181 YILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLVDCD 240
           YILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLVDCD
Sbjct: 181 YILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLVDCD 240

Query: 241 PYGFDILTTYRFGSMVRLLN 260
           PYGFDILTTYRFGSMVRLLN
Sbjct: 241 PYGFDILTTYRFGSMVRLLN 260

BLAST of Csor.00g036830 vs. NCBI nr
Match: KAG7024967.1 (Meiotic recombination protein SPO11-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 498 bits (1282), Expect = 3.28e-177
Identity = 255/268 (95.15%), Postives = 256/268 (95.52%), Query Frame = 0

Query: 1   MAGKLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENW---FNLSE 60
           MAG LGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNS EN    FNLSE
Sbjct: 1   MAGNLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           GK+ILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN
Sbjct: 61  GKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNN-----AHSIPVQVEEV 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNN     AHSIPVQVEEV
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEV 180

Query: 181 TDIASPANYILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240
           TDIASPANYILVVEKESVFQRLANDHFC RNRCIVISGRGYPDVPTRRFLRLLVDVLALP
Sbjct: 181 TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240

Query: 241 AFCLVDCDPYGFDILTTYRFGSMVRLLN 260
           AFCLVDCDPYGFDILTTYRFGSMVRLLN
Sbjct: 241 AFCLVDCDPYGFDILTTYRFGSMVRLLN 268

BLAST of Csor.00g036830 vs. NCBI nr
Match: XP_022937001.1 (meiotic recombination protein SPO11-1 [Cucurbita moschata])

HSP 1 Score: 490 bits (1261), Expect = 2.01e-172
Identity = 249/260 (95.77%), Postives = 251/260 (96.54%), Query Frame = 0

Query: 1   MAGKLGSSERI--DIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENW---FNL 60
           MAGKLGSSERI  DIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTEN    FNL
Sbjct: 1   MAGKLGSSERIRIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENCCCRFNL 60

Query: 61  SEGKEILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRA 120
           SEGKEILTLQ++SHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQS VDRA
Sbjct: 61  SEGKEILTLQRQSHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSAVDRA 120

Query: 121 INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDI 180
           INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDI
Sbjct: 121 INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDI 180

Query: 181 ASPANYILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFC 240
           A PANYILVVEKESVFQRLANDHFC RNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFC
Sbjct: 181 AGPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFC 240

Query: 241 LVDCDPYGFDILTTYRFGSM 255
           LVDCDPYGFDILTTYRFGSM
Sbjct: 241 LVDCDPYGFDILTTYRFGSM 260

BLAST of Csor.00g036830 vs. NCBI nr
Match: XP_023536168.1 (meiotic recombination protein SPO11-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 487 bits (1253), Expect = 3.08e-171
Identity = 247/258 (95.74%), Postives = 249/258 (96.51%), Query Frame = 0

Query: 1   MAGKLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENW---FNLSE 60
           MAGKLGSSERIDIMRKIRELTRSMIVEL N SSKMIAIDRFKNYCTNS EN    FNLSE
Sbjct: 1   MAGKLGSSERIDIMRKIRELTRSMIVELCNRSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           GKEILTLQ+ESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN
Sbjct: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIAS 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRR DCIKKPN+AHSIPVQVEEVTDIAS
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRSDCIKKPNSAHSIPVQVEEVTDIAS 180

Query: 181 PANYILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
           PANYILVVEKESVFQRLANDHFC RNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV
Sbjct: 181 PANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFDILTTYRFGSM 255
           DCDPYGFDILTTYRFGSM
Sbjct: 241 DCDPYGFDILTTYRFGSM 258

BLAST of Csor.00g036830 vs. NCBI nr
Match: XP_022976331.1 (meiotic recombination protein SPO11-1 [Cucurbita maxima])

HSP 1 Score: 486 bits (1250), Expect = 7.66e-171
Identity = 246/258 (95.35%), Postives = 248/258 (96.12%), Query Frame = 0

Query: 1   MAGKLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENW---FNLSE 60
           MAG LG SERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYC NS EN    FNLSE
Sbjct: 1   MAGNLGRSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCINSIENCCCRFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           GKEILTLQ+ESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN
Sbjct: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIAS 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRR DCIKKPN+AHSIPVQVEEVTDIAS
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRSDCIKKPNSAHSIPVQVEEVTDIAS 180

Query: 181 PANYILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
           PANYILVVEKESVFQRLANDHFC RNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV
Sbjct: 181 PANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFDILTTYRFGSM 255
           DCDPYGFDILTTYRFGSM
Sbjct: 241 DCDPYGFDILTTYRFGSM 258

BLAST of Csor.00g036830 vs. ExPASy TrEMBL
Match: A0A6J1F921 (meiotic recombination protein SPO11-1 OS=Cucurbita moschata OX=3662 GN=LOC111443423 PE=3 SV=1)

HSP 1 Score: 490 bits (1261), Expect = 9.71e-173
Identity = 249/260 (95.77%), Postives = 251/260 (96.54%), Query Frame = 0

Query: 1   MAGKLGSSERI--DIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENW---FNL 60
           MAGKLGSSERI  DIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTEN    FNL
Sbjct: 1   MAGKLGSSERIRIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENCCCRFNL 60

Query: 61  SEGKEILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRA 120
           SEGKEILTLQ++SHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQS VDRA
Sbjct: 61  SEGKEILTLQRQSHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSAVDRA 120

Query: 121 INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDI 180
           INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDI
Sbjct: 121 INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDI 180

Query: 181 ASPANYILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFC 240
           A PANYILVVEKESVFQRLANDHFC RNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFC
Sbjct: 181 AGPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFC 240

Query: 241 LVDCDPYGFDILTTYRFGSM 255
           LVDCDPYGFDILTTYRFGSM
Sbjct: 241 LVDCDPYGFDILTTYRFGSM 260

BLAST of Csor.00g036830 vs. ExPASy TrEMBL
Match: A0A6J1IGM0 (meiotic recombination protein SPO11-1 OS=Cucurbita maxima OX=3661 GN=LOC111476764 PE=3 SV=1)

HSP 1 Score: 486 bits (1250), Expect = 3.71e-171
Identity = 246/258 (95.35%), Postives = 248/258 (96.12%), Query Frame = 0

Query: 1   MAGKLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENW---FNLSE 60
           MAG LG SERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYC NS EN    FNLSE
Sbjct: 1   MAGNLGRSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCINSIENCCCRFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           GKEILTLQ+ESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN
Sbjct: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIAS 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRR DCIKKPN+AHSIPVQVEEVTDIAS
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRSDCIKKPNSAHSIPVQVEEVTDIAS 180

Query: 181 PANYILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
           PANYILVVEKESVFQRLANDHFC RNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV
Sbjct: 181 PANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFDILTTYRFGSM 255
           DCDPYGFDILTTYRFGSM
Sbjct: 241 DCDPYGFDILTTYRFGSM 258

BLAST of Csor.00g036830 vs. ExPASy TrEMBL
Match: A0A1S3CI64 (meiotic recombination protein SPO11-1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501238 PE=3 SV=1)

HSP 1 Score: 469 bits (1206), Expect = 3.74e-164
Identity = 229/255 (89.80%), Postives = 244/255 (95.69%), Query Frame = 0

Query: 1   MAGKLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENWFNLSEGKE 60
           M G  GS ERI+++RKI ELTRSMIVELSNGSSKMIAIDRFK+YCTNS ENWFNLSEGK+
Sbjct: 1   MEGNRGSLERIEVLRKITELTRSMIVELSNGSSKMIAIDRFKSYCTNSLENWFNLSEGKD 60

Query: 61  ILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAINDIC 120
           +LTLQ+ESHVRRLDVLLRVLLIIQQLLRENRHSSKRD+YYMHPS+FTDQSVVDRAINDIC
Sbjct: 61  VLTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDMYYMHPSIFTDQSVVDRAINDIC 120

Query: 121 ILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIASPAN 180
           ILLQCSRHNLNVVSVGNGLVMGWLRF+EAGRRFDC+KKPN+A  IPVQVEEVTDIA+ A+
Sbjct: 121 ILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAQFIPVQVEEVTDIATAAD 180

Query: 181 YILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLVDCD 240
           YILVVEKESVFQRLAND FC RNRCIVI+GRGYPDVPTRRFLRLLVDVLALPAFCLVDCD
Sbjct: 181 YILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLVDCD 240

Query: 241 PYGFDILTTYRFGSM 255
           PYGFDILTTYRFGSM
Sbjct: 241 PYGFDILTTYRFGSM 255

BLAST of Csor.00g036830 vs. ExPASy TrEMBL
Match: A0A6J1CDC7 (meiotic recombination protein SPO11-1 OS=Momordica charantia OX=3673 GN=LOC111010510 PE=3 SV=1)

HSP 1 Score: 461 bits (1186), Expect = 2.19e-161
Identity = 230/258 (89.15%), Postives = 242/258 (93.80%), Query Frame = 0

Query: 1   MAGKLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENW---FNLSE 60
           M GK  SSER DI+R+IRE+TRSMIVELSNGSSKM+AIDRFKNYCTNS EN    FNLSE
Sbjct: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           GKEILTLQKESHVRRLDVLLRVLLI++QLLRENRHSSKRDIYYMHPSVF+DQSVVDRAIN
Sbjct: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIAS 180
           DICILLQCSRHNLNVVS GNGLVMGWLRF+EAGRRFDCIKKPN+AHSIPVQVEEVTDI S
Sbjct: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180

Query: 181 PANYILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
            A YIL+VEKESVFQRLAND FC RNRCIVI+GRGYPDVPTRRFLRLLVDV ALPAFCLV
Sbjct: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240

Query: 241 DCDPYGFDILTTYRFGSM 255
           DCDPYGF+ILTTYRFGSM
Sbjct: 241 DCDPYGFEILTTYRFGSM 258

BLAST of Csor.00g036830 vs. ExPASy TrEMBL
Match: A0A1S3CJR6 (meiotic recombination protein SPO11-1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501238 PE=3 SV=1)

HSP 1 Score: 458 bits (1179), Expect = 5.33e-160
Identity = 228/258 (88.37%), Postives = 243/258 (94.19%), Query Frame = 0

Query: 1   MAGKLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENW---FNLSE 60
           M G  GS ERI+++RKI ELTRSMIVELSNGSSKMIAIDRFK+YCTNS EN    FNLSE
Sbjct: 1   MEGNRGSLERIEVLRKITELTRSMIVELSNGSSKMIAIDRFKSYCTNSLENCCCRFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           GK++LTLQ+ESHVRRLDVLLRVLLIIQQLLRENRHSSKRD+YYMHPS+FTDQSVVDRAIN
Sbjct: 61  GKDVLTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDMYYMHPSIFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIAS 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRF+EAGRRFDC+KKPN+A  IPVQVEEVTDIA+
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAQFIPVQVEEVTDIAT 180

Query: 181 PANYILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
            A+YILVVEKESVFQRLAND FC RNRCIVI+GRGYPDVPTRRFLRLLVDVLALPAFCLV
Sbjct: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFDILTTYRFGSM 255
           DCDPYGFDILTTYRFGSM
Sbjct: 241 DCDPYGFDILTTYRFGSM 258

BLAST of Csor.00g036830 vs. TAIR 10
Match: AT3G13170.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 347.4 bits (890), Expect = 9.7e-96
Identity = 169/258 (65.50%), Postives = 206/258 (79.84%), Query Frame = 0

Query: 1   MAGKLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENWFNLSE--- 60
           M GK   SE  +++++I++ T+S++V+L+ G S  I+I++F+NYC N   +    S+   
Sbjct: 1   MEGKFAISESTNLLQRIKDFTQSVVVDLAEGRSPKISINQFRNYCMNPEADCLCSSDKPK 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           G+EI TL+KE    R+D+LLRVLLI+QQLL+ENRH+SKRDIYYMHPS F  QS+VDRAI 
Sbjct: 61  GQEIFTLKKEPQTYRIDMLLRVLLIVQQLLQENRHASKRDIYYMHPSAFKAQSIVDRAIG 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIAS 180
           DICIL QCSR+NLNVVSVGNGLVMGWL+F EAGR+FDC+   N A+ +PV VEEV DI S
Sbjct: 121 DICILFQCSRYNLNVVSVGNGLVMGWLKFREAGRKFDCLNSLNTAYPVPVLVEEVEDIVS 180

Query: 181 PANYILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
            A YILVVEKE+VFQRLAND FC  NRCIVI+GRGYPDV TRRFLRLL++ L LP  CLV
Sbjct: 181 LAEYILVVEKETVFQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKLHLPVHCLV 240

Query: 241 DCDPYGFDILTTYRFGSM 256
           DCDPYGF+IL TYRFGSM
Sbjct: 241 DCDPYGFEILATYRFGSM 258

BLAST of Csor.00g036830 vs. TAIR 10
Match: AT5G02820.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 157.9 bits (398), Expect = 1.1e-38
Identity = 77/187 (41.18%), Postives = 113/187 (60.43%), Query Frame = 0

Query: 68  SHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAINDICILLQCSR 127
           S VR+  +  R+L +I QL   N H +KRD++Y    +F DQ+  D  ++D+  +L C+R
Sbjct: 138 SSVRKTTITTRILALIHQLCLRNIHVTKRDLFYTDVKLFQDQTQSDAVLDDVSCMLGCTR 197

Query: 128 HNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDIASPANYILVVEK 187
            +LNV++   G+V+G L F++ G   DC K      +IP  ++ V D+ S A +IL+VEK
Sbjct: 198 SSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQSDAMFILLVEK 257

Query: 188 ESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLVDCDPYGFDIL 247
           ++ + RLA D F  R  CI+++ +G PDV TR FLR +   L LP   LVD DPYG  IL
Sbjct: 258 DAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALVDSDPYGLKIL 317

Query: 248 TTYRFGS 255
           + Y  GS
Sbjct: 318 SVYGCGS 324

BLAST of Csor.00g036830 vs. TAIR 10
Match: AT1G63990.1 (sporulation 11-2 )

HSP 1 Score: 112.8 bits (281), Expect = 4.0e-25
Identity = 63/186 (33.87%), Postives = 106/186 (56.99%), Query Frame = 0

Query: 78  RVLLIIQQLLRENRHSSKRDIYYM----HPSVFTDQSVVDRAINDICILLQCSRHNLNVV 137
           +V+ I  Q+L + +  ++R+++Y      P  F+ Q  V+R++ D+  LL+CSR++L ++
Sbjct: 102 KVMEICFQILLQEKRVTQRELFYKLLCDSPDYFSSQIEVNRSVQDVVALLRCSRYSLGIM 161

Query: 138 SVGNGLVMGWLRFAEAGRR-FDCIKKPNNAHSI--PVQVEEVTDIASPANYILVVEKESV 197
           +   GLV G L   E G+   DC    ++  +I   + + + T + + A YI++VEK ++
Sbjct: 162 ASSRGLVAGRLFLQEPGKEAVDCSACGSSGFAITGDLNLLDNTIMRTDARYIIIVEKHAI 221

Query: 198 FQRLANDHFCGRNRCIVISGRGYPDVPTRRFL-RLLVDVLALPAFCLVDCDPYGFDILTT 256
           F RL  D       C+ I+ +GYPD+ TR FL R+      LP   LVD +P G  IL T
Sbjct: 222 FHRLVEDRVFNHIPCVFITAKGYPDIATRFFLHRMSTTFPDLPILVLVDWNPAGLAILCT 281

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M4A21.4e-9465.50Meiotic recombination protein SPO11-1 OS=Arabidopsis thaliana OX=3702 GN=SPO11-1... [more]
Q5ZPV82.0e-8256.86Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Q7Y0212.0e-8256.86Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9LZ031.5e-3741.18DNA topoisomerase 6 subunit A OS=Arabidopsis thaliana OX=3702 GN=TOP6A PE=1 SV=1[more]
Q9Y5K15.8e-3732.17Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6592090.16.08e-187100.00Meiotic recombination protein SPO11-1, partial [Cucurbita argyrosperma subsp. so... [more]
KAG7024967.13.28e-17795.15Meiotic recombination protein SPO11-1, partial [Cucurbita argyrosperma subsp. ar... [more]
XP_022937001.12.01e-17295.77meiotic recombination protein SPO11-1 [Cucurbita moschata][more]
XP_023536168.13.08e-17195.74meiotic recombination protein SPO11-1 [Cucurbita pepo subsp. pepo][more]
XP_022976331.17.66e-17195.35meiotic recombination protein SPO11-1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1F9219.71e-17395.77meiotic recombination protein SPO11-1 OS=Cucurbita moschata OX=3662 GN=LOC111443... [more]
A0A6J1IGM03.71e-17195.35meiotic recombination protein SPO11-1 OS=Cucurbita maxima OX=3661 GN=LOC11147676... [more]
A0A1S3CI643.74e-16489.80meiotic recombination protein SPO11-1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1CDC72.19e-16189.15meiotic recombination protein SPO11-1 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A1S3CJR65.33e-16088.37meiotic recombination protein SPO11-1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT3G13170.19.7e-9665.50Spo11/DNA topoisomerase VI, subunit A protein [more]
AT5G02820.11.1e-3841.18Spo11/DNA topoisomerase VI, subunit A protein [more]
AT1G63990.14.0e-2533.87sporulation 11-2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002815Spo11/DNA topoisomerase VI subunit APRINTSPR01550TOP6AFAMILYcoord: 95..106
score: 50.0
coord: 238..254
score: 52.57
coord: 206..227
score: 42.9
coord: 109..126
score: 43.06
coord: 182..199
score: 54.17
coord: 127..142
score: 41.41
IPR002815Spo11/DNA topoisomerase VI subunit APANTHERPTHR10848MEIOTIC RECOMBINATION PROTEIN SPO11coord: 8..256
IPR013049Spo11/DNA topoisomerase VI, subunit A, N-terminalPFAMPF04406TP6A_Ncoord: 71..132
e-value: 3.8E-22
score: 78.1
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 2..135
e-value: 2.5E-24
score: 87.7
NoneNo IPR availableGENE3D3.40.1360.10coord: 137..259
e-value: 3.4E-47
score: 162.8
NoneNo IPR availablePANTHERPTHR10848:SF3MEIOTIC RECOMBINATION PROTEIN SPO11-1coord: 8..256
IPR034136Topoisomerase 6 subunit A/Spo11, TOPRIM domainCDDcd00223TOPRIM_TopoIIB_SPOcoord: 180..255
e-value: 1.3334E-37
score: 128.141
IPR036078Spo11/DNA topoisomerase VI subunit A superfamilySUPERFAMILY56726DNA topoisomerase IV, alpha subunitcoord: 70..255

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g036830.m01Csor.00g036830.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051026 chiasma assembly
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0042138 meiotic DNA double-strand break formation
biological_process GO:0006259 DNA metabolic process
cellular_component GO:0005694 chromosome
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003824 catalytic activity