Csor.00g036810 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g036810
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptiontwo-pore potassium channel 1
LocationCsor_Chr09: 5367015 .. 5368382 (+)
RNA-Seq ExpressionCsor.00g036810
SyntenyCsor.00g036810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTACCAACATCATCTAATACCTTCGTAACAAGGTCAATAGATATTCCGAGAAGCAAAAGACGATTGAGGCGCACGAAGAGTGCTCCTCATTCGGATTCTCCTCATACAGAGACCACTGGCACCGTTACTGGTCTAGTTCCACGTTCAGGATTAGTTTTCGGAAATCTACACCCGAGTTTTATAAGAGTAGCCTTAGTTTTAATAATGTACTTGGGTATAGGAACTCTATGTTTCTACCTTGTCAGGCACCAAATCAAAGGGGGAAAAACCAACGGAGTAGTTGATGCCATTTATTTCACTATTGTGACAATGACAACCGTTGGATATGGTGATCTTGTCCCAAACACTCCTTCCACCAAGCTACTAGCTTGTGCTTTTGTCTTCACAGGAATGGCCGTAGTTGGCCTAATATTAACCAATGCAGCTGATTATTTGGTAGAGAAGCAGGAAATCTTTCTATTTAAGGTCTTCCATATGCACCAAAATGGTTCTTGCGACATTACAGAAGAAATTGATACTAATAAAGCTAGAAATAAGTGTATAGTGGTGTTTCTTTTTCTTCTTTTGTTCATAATTTTTGGGACTGTCTTTCTAGTTACCCTTGAGGAGTTGGATTTTATTGATGCCTTTTATTGTGTATGTTCTACCATTACTACGCTAGGCTATGGAGATAAAAGCTTCTCGACCCGGTGGGGGCGTCTATTCGCCATTTTCTGGATCTTGATAAGTTCCATCACTCTGGCTCAGTTTTTCCTTTATATTGCTGTGCTAAACACTGAGAGAAGGAAAAAATCTCTGGTGAAGTGGGTTCTAAGTAAAAAGGTGACGGTCTTGGACCTCGAGGCTGCGGATCTCGATGATGATGGGGTCGTCGGGTAAGTCATAGTTTGTTCTTTTCATTAGACTTCACTATCAGGTGGCCTCATTGTGAGATCCTACATTGGTTGGGGAGGAGAACGAAACATTTTTTATAAGAGTGTGGAAACCTTCCTCTCGCAGACGCGATTTAAAAACCTTGAGGGAAAGTCCGAAAGAGAAAGTTCAAAGAGGACAATATCTGCTAGCGGTGGGCTTGGGCTATTACACTCTTACTCACTCCGAACACACCCATTCGTTTTTTATGAGACCTCTAGCATGTTTACGAGATTCACATACGTTTTCGAGTTGGAGTTTATTAAATTTCCATTGATCATTTCCTTCTTAAACTTCCACAGGGCTGCAGAATTTGTCATATACAAGCTCAAGGAGATGGGGAAAATCACTGAAGATGACATTTCACCTGTTCTGGACGAATTTGAGAATCTTGATGTTGATCAATCAGGAACATTGTCCATATCTGATATAACACTTGCTCGATCGTCTTAA

mRNA sequence

ATGTTACCAACATCATCTAATACCTTCGTAACAAGGTCAATAGATATTCCGAGAAGCAAAAGACGATTGAGGCGCACGAAGAGTGCTCCTCATTCGGATTCTCCTCATACAGAGACCACTGGCACCGTTACTGGTCTAGTTCCACGTTCAGGATTAGTTTTCGGAAATCTACACCCGAGTTTTATAAGAGTAGCCTTAGTTTTAATAATGTACTTGGGTATAGGAACTCTATGTTTCTACCTTGTCAGGCACCAAATCAAAGGGGGAAAAACCAACGGAGTAGTTGATGCCATTTATTTCACTATTGTGACAATGACAACCGTTGGATATGGTGATCTTGTCCCAAACACTCCTTCCACCAAGCTACTAGCTTGTGCTTTTGTCTTCACAGGAATGGCCGTAGTTGGCCTAATATTAACCAATGCAGCTGATTATTTGGTAGAGAAGCAGGAAATCTTTCTATTTAAGGTCTTCCATATGCACCAAAATGGTTCTTGCGACATTACAGAAGAAATTGATACTAATAAAGCTAGAAATAAGTGTATAGTGGTGTTTCTTTTTCTTCTTTTGTTCATAATTTTTGGGACTGTCTTTCTAGTTACCCTTGAGGAGTTGGATTTTATTGATGCCTTTTATTGTGTATGTTCTACCATTACTACGCTAGGCTATGGAGATAAAAGCTTCTCGACCCGGTGGGGGCGTCTATTCGCCATTTTCTGGATCTTGATAAGTTCCATCACTCTGGCTCAGTTTTTCCTTTATATTGCTGTGCTAAACACTGAGAGAAGGAAAAAATCTCTGGTGAAGTGGGTTCTAAGTAAAAAGGTGACGGTCTTGGACCTCGAGGCTGCGGATCTCGATGATGATGGGGTCGTCGGGGCTGCAGAATTTGTCATATACAAGCTCAAGGAGATGGGGAAAATCACTGAAGATGACATTTCACCTGTTCTGGACGAATTTGAGAATCTTGATGTTGATCAATCAGGAACATTGTCCATATCTGATATAACACTTGCTCGATCGTCTTAA

Coding sequence (CDS)

ATGTTACCAACATCATCTAATACCTTCGTAACAAGGTCAATAGATATTCCGAGAAGCAAAAGACGATTGAGGCGCACGAAGAGTGCTCCTCATTCGGATTCTCCTCATACAGAGACCACTGGCACCGTTACTGGTCTAGTTCCACGTTCAGGATTAGTTTTCGGAAATCTACACCCGAGTTTTATAAGAGTAGCCTTAGTTTTAATAATGTACTTGGGTATAGGAACTCTATGTTTCTACCTTGTCAGGCACCAAATCAAAGGGGGAAAAACCAACGGAGTAGTTGATGCCATTTATTTCACTATTGTGACAATGACAACCGTTGGATATGGTGATCTTGTCCCAAACACTCCTTCCACCAAGCTACTAGCTTGTGCTTTTGTCTTCACAGGAATGGCCGTAGTTGGCCTAATATTAACCAATGCAGCTGATTATTTGGTAGAGAAGCAGGAAATCTTTCTATTTAAGGTCTTCCATATGCACCAAAATGGTTCTTGCGACATTACAGAAGAAATTGATACTAATAAAGCTAGAAATAAGTGTATAGTGGTGTTTCTTTTTCTTCTTTTGTTCATAATTTTTGGGACTGTCTTTCTAGTTACCCTTGAGGAGTTGGATTTTATTGATGCCTTTTATTGTGTATGTTCTACCATTACTACGCTAGGCTATGGAGATAAAAGCTTCTCGACCCGGTGGGGGCGTCTATTCGCCATTTTCTGGATCTTGATAAGTTCCATCACTCTGGCTCAGTTTTTCCTTTATATTGCTGTGCTAAACACTGAGAGAAGGAAAAAATCTCTGGTGAAGTGGGTTCTAAGTAAAAAGGTGACGGTCTTGGACCTCGAGGCTGCGGATCTCGATGATGATGGGGTCGTCGGGGCTGCAGAATTTGTCATATACAAGCTCAAGGAGATGGGGAAAATCACTGAAGATGACATTTCACCTGTTCTGGACGAATTTGAGAATCTTGATGTTGATCAATCAGGAACATTGTCCATATCTGATATAACACTTGCTCGATCGTCTTAA

Protein sequence

MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPSFIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPSTKLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNKARNKCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLARSS
Homology
BLAST of Csor.00g036810 vs. ExPASy Swiss-Prot
Match: Q8LBL1 (Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2)

HSP 1 Score: 378.6 bits (971), Expect = 7.3e-104
Identity = 201/344 (58.43%), Postives = 258/344 (75.00%), Query Frame = 0

Query: 2   LPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLV--FGNLHP 61
           + T +  F   S      KRRLRR++SAP  D  + +    +    P    +  F +L+P
Sbjct: 16  IDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDV-KIDEPPPHPSKIPMFSDLNP 75

Query: 62  SFIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPS 121
           +  RV + L +YL IGTLCFYLVR QI G KT+GVVDA+YF IVTMTTVGYGDLVPN+ +
Sbjct: 76  NLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGDLVPNSSA 135

Query: 122 TKLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQN-GSCDITEEIDTNKAR 181
           ++LLACAFVF+GM +VG +L+ AADYLVEKQE  L + FH+ Q+ G  DI +E+ TNK R
Sbjct: 136 SRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQSFGPTDILKELHTNKLR 195

Query: 182 NKCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAI 241
            KC    L L++  I GT+FLV +E++  I AFYCVCST+TTLGYGDKSF++  GRLFA+
Sbjct: 196 YKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTTLGYGDKSFNSEAGRLFAV 255

Query: 242 FWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFV 301
           FWIL SSI LAQFFLY+A LNTE ++++LVKWVL++++T  DLEAADLD+DGVVGAAEF+
Sbjct: 256 FWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLDEDGVVGAAEFI 315

Query: 302 IYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLARSS 343
           +YKLKEMGKI E DIS ++DEFE LD D+SGTL+ SDI LA+++
Sbjct: 316 VYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIVLAQTT 358

BLAST of Csor.00g036810 vs. ExPASy Swiss-Prot
Match: Q850M0 (Two pore potassium channel a OS=Oryza sativa subsp. japonica OX=39947 GN=TPKA PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.2e-90
Identity = 179/322 (55.59%), Postives = 233/322 (72.36%), Query Frame = 0

Query: 22  RLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPSFIRVALVLIMYLGIGTLCFYL 81
           R RR +S P +D           G   ++  +F  + PSF  V L+L +YL +G L FY 
Sbjct: 28  RFRRCRSTPSTDPLQGPPE---KGSSVKAKELFKEMRPSFRLVGLLLFIYLLVGVLAFYA 87

Query: 82  VRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPSTKLLACAFVFTGMAVVGLILTN 141
           V  +I G +TN V+DA+YF +VTMTTVGYGDLVPN  +TKLLACAFVF GMAVV L ++ 
Sbjct: 88  VMDEISGKRTNRVLDALYFCVVTMTTVGYGDLVPNNDTTKLLACAFVFMGMAVVALFVSK 147

Query: 142 AADYLVEKQEIFLFKVFHMH-QNGSCDITEEIDTNKARNKCIVVFLFLLLFIIFGTVFLV 201
            ADYLVEKQE+  FK  H + + G   +   I+TN+ + K     L L+L II GTVFL 
Sbjct: 148 VADYLVEKQEVLFFKALHTNLKGGETKMLRAIETNRIKYKFYTNALLLVLSIISGTVFLW 207

Query: 202 TLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFWILISSITLAQFFLYIAVLNT 261
            +E+L  +D+FYCVC+TITTLGYGDKSFS++ GR+FA+FWI+ S+I +AQFF+Y+A + T
Sbjct: 208 KVEKLSLVDSFYCVCATITTLGYGDKSFSSKLGRVFAVFWIITSTIIMAQFFMYLAEIYT 267

Query: 262 ERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIYKLKEMGKITEDDISPVLDEF 321
           ERR+K L  WVL++K+T +DLEAADLDDD  VGAAEFV+YKLKE+GKI +++IS  L+EF
Sbjct: 268 ERRQKMLANWVLTRKMTKMDLEAADLDDDRQVGAAEFVVYKLKELGKINQEEISSFLEEF 327

Query: 322 ENLDVDQSGTLSISDITLARSS 343
           E LDVD SGTLS  D+TLA+S+
Sbjct: 328 EKLDVDHSGTLSPYDLTLAQSA 346

BLAST of Csor.00g036810 vs. ExPASy Swiss-Prot
Match: Q8LIN5 (Two pore potassium channel b OS=Oryza sativa subsp. japonica OX=39947 GN=TPKB PE=1 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 6.4e-84
Identity = 166/320 (51.88%), Postives = 218/320 (68.12%), Query Frame = 0

Query: 21  RRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPSFIRVALVLIMYLGIGTLCFY 80
           RR RR ++AP S+ P T+   +     P   L  G   PSF  V L+L+ YL +GT+ FY
Sbjct: 25  RRFRRCRTAPSSEPPPTDKDNSSAADAPPKTLFTGGGRPSFRLVGLLLVAYLLLGTIAFY 84

Query: 81  LVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPSTKLLACAFVFTGMAVVGLILT 140
           L    + G +T   +DA+YF +VTMTTVGYGDLVP + + KLLACAFVF G+AVVG  L+
Sbjct: 85  LAMDHMSGTRTTRALDALYFCVVTMTTVGYGDLVPASDAAKLLACAFVFAGVAVVGTFLS 144

Query: 141 NAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNKARNKCIVVFLFLLLFIIFGTVFLV 200
            AADYLVEKQE  LF+  H H      +   ++ NK R K     L L+  +  GTV L 
Sbjct: 145 KAADYLVEKQEALLFRALHSH-----TMVRAMEMNKVRYKLYTAGLLLVAAVASGTVVLW 204

Query: 201 TLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFWILISSITLAQFFLYIAVLNT 260
            +E +  +DAFYCVC+T+TTLGYGD+SFS+  GR FA+ WI +S++ +A FFLY A L T
Sbjct: 205 KVEGMRAVDAFYCVCATVTTLGYGDRSFSSEGGRAFAVAWITVSTVVVALFFLYAAELYT 264

Query: 261 ERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIYKLKEMGKITEDDISPVLDEF 320
           ERR++ L +WVL ++ T +DLEAADLD D  VGAA+FV+YKLKE+GKI+++DIS  LDEF
Sbjct: 265 ERRQRELARWVLRRRTTNMDLEAADLDGDHRVGAADFVLYKLKELGKISQEDISEFLDEF 324

Query: 321 ENLDVDQSGTLSISDITLAR 341
           +NLD D SGTLS +D+  A+
Sbjct: 325 DNLDADHSGTLSPADLAAAQ 339

BLAST of Csor.00g036810 vs. ExPASy Swiss-Prot
Match: Q9S6Z8 (Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.5e-45
Identity = 110/289 (38.06%), Postives = 172/289 (59.52%), Query Frame = 0

Query: 60  SFIRVAL-VLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTP 119
           S IR A+ +LI+YL +G   +   R    G +T+ VVDA+YF IVTM T+GYGD+ P TP
Sbjct: 115 SIIRQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTP 174

Query: 120 STKLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLF---------KVFHMHQNGSCD-- 179
            TK+ A  FV  G   + ++L+   +Y+++ QE  +          +  H H+  + D  
Sbjct: 175 WTKIFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYI 234

Query: 180 ITEEIDTNKARNKCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKS 239
           I  E    + R K  +    ++L I  G + L  +EEL F+D+ Y    ++TT+GYGD++
Sbjct: 235 IDFEKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRA 294

Query: 240 FSTRWGRLFAIFWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLD 299
           F T  GRLFA  W+L+S++ +A+ FLY+A    +RR +  VK  L++++TV DL  AD  
Sbjct: 295 FKTLQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTY 354

Query: 300 DDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDI 337
             G +  +E+++ KLKEMGKIT+ DI  V+ +FE LD +Q G +++ D+
Sbjct: 355 QHGFISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPDL 403

BLAST of Csor.00g036810 vs. ExPASy Swiss-Prot
Match: Q9SVV6 (Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.7e-44
Identity = 103/282 (36.52%), Postives = 167/282 (59.22%), Query Frame = 0

Query: 60  SFIRVAL-VLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTP 119
           S +R A  +L++YL +G L ++L R      +T+ VVD +YF IVTM T+GYGD+ PN+ 
Sbjct: 148 SVVRQAFALLVVYLSLGVLIYWLNRDHYVVNQTHPVVDGLYFCIVTMCTIGYGDITPNSV 207

Query: 120 STKLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNKAR 179
            TKL +  FV  G   + ++L+    Y+++ QE ++              +  ID  K R
Sbjct: 208 VTKLFSIMFVLVGFGFIDILLSGMVSYVLDLQESYMLDSAKRRDEPEKRRSYIIDVKKGR 267

Query: 180 N----KCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGR 239
                K  +    ++L I  G   +  +EE+ ++D+FY    ++TT+GYGD++F T  GR
Sbjct: 268 MRIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLDSFYLSVMSVTTVGYGDRAFKTLPGR 327

Query: 240 LFAIFWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGA 299
           LFA  W+L+S++ +A+ FLY+A    ++R +   K VL + ++V    AAD+D++G V  
Sbjct: 328 LFAAIWLLVSTLAVARAFLYLAEARVDKRNRERAKKVLCETMSVSQFFAADIDNNGCVSK 387

Query: 300 AEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDI 337
           AE+VIYKLKEM KIT+ DI P+  +F+ LD   +G +++ D+
Sbjct: 388 AEYVIYKLKEMEKITDKDILPISKQFDKLDRCSNGKITLLDL 429

BLAST of Csor.00g036810 vs. NCBI nr
Match: KAG6592092.1 (Two-pore potassium channel 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 660 bits (1704), Expect = 6.63e-239
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 0

Query: 1   MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPS 60
           MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPS
Sbjct: 1   MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPS 60

Query: 61  FIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST 120
           FIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST
Sbjct: 61  FIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST 120

Query: 121 KLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNKARNK 180
           KLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNKARNK
Sbjct: 121 KLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNKARNK 180

Query: 181 CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW 240
           CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW
Sbjct: 181 CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW 240

Query: 241 ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY 300
           ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY
Sbjct: 241 ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY 300

Query: 301 KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLARSS 342
           KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLARSS
Sbjct: 301 KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLARSS 342

BLAST of Csor.00g036810 vs. NCBI nr
Match: KAG7024969.1 (Two-pore potassium channel 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 659 bits (1700), Expect = 3.78e-238
Identity = 341/342 (99.71%), Postives = 342/342 (100.00%), Query Frame = 0

Query: 1   MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPS 60
           MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPS
Sbjct: 10  MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPS 69

Query: 61  FIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST 120
           FIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST
Sbjct: 70  FIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST 129

Query: 121 KLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNKARNK 180
           KLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNKARNK
Sbjct: 130 KLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNKARNK 189

Query: 181 CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW 240
           CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW
Sbjct: 190 CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW 249

Query: 241 ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY 300
           ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY
Sbjct: 250 ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY 309

Query: 301 KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLARSS 342
           KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLA+SS
Sbjct: 310 KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQSS 351

BLAST of Csor.00g036810 vs. NCBI nr
Match: XP_023535214.1 (two-pore potassium channel 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 654 bits (1686), Expect = 5.14e-236
Identity = 337/342 (98.54%), Postives = 340/342 (99.42%), Query Frame = 0

Query: 1   MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPS 60
           MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPS
Sbjct: 10  MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPS 69

Query: 61  FIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST 120
           FIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDA+YFTIVTMTTVGYGDLVPNTPST
Sbjct: 70  FIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAVYFTIVTMTTVGYGDLVPNTPST 129

Query: 121 KLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNKARNK 180
           KLLACAFVFTGMAVVGLIL NAADYLVEKQEIFLFK FHMHQNGSCDITEEIDTNKARNK
Sbjct: 130 KLLACAFVFTGMAVVGLILINAADYLVEKQEIFLFKAFHMHQNGSCDITEEIDTNKARNK 189

Query: 181 CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW 240
           CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW
Sbjct: 190 CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW 249

Query: 241 ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY 300
           ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY
Sbjct: 250 ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY 309

Query: 301 KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLARSS 342
           KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITL++SS
Sbjct: 310 KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLSQSS 351

BLAST of Csor.00g036810 vs. NCBI nr
Match: XP_022936020.1 (two-pore potassium channel 1 [Cucurbita moschata])

HSP 1 Score: 640 bits (1652), Expect = 7.81e-231
Identity = 333/342 (97.37%), Postives = 335/342 (97.95%), Query Frame = 0

Query: 1   MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPS 60
           MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHS SPHTETT T TGLVPRSGLVFGNLHPS
Sbjct: 10  MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSGSPHTETTSTATGLVPRSGLVFGNLHPS 69

Query: 61  FIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST 120
           FIRVALVLIMYLGIGTLCFYLV HQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST
Sbjct: 70  FIRVALVLIMYLGIGTLCFYLVGHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST 129

Query: 121 KLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNKARNK 180
           KLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFK FHMHQNG  DIT+EIDTNKARNK
Sbjct: 130 KLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKAFHMHQNGPFDITKEIDTNKARNK 189

Query: 181 CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW 240
           CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW
Sbjct: 190 CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW 249

Query: 241 ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY 300
           ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY
Sbjct: 250 ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY 309

Query: 301 KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLARSS 342
           KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLA+SS
Sbjct: 310 KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQSS 351

BLAST of Csor.00g036810 vs. NCBI nr
Match: XP_022975856.1 (two-pore potassium channel 1-like [Cucurbita maxima])

HSP 1 Score: 635 bits (1639), Expect = 7.48e-229
Identity = 330/342 (96.49%), Postives = 334/342 (97.66%), Query Frame = 0

Query: 1   MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPS 60
           MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHS SPHTETT T TGLVPRSGLVFGNLHPS
Sbjct: 10  MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSGSPHTETTSTATGLVPRSGLVFGNLHPS 69

Query: 61  FIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST 120
           FIRVALVLIMYLGIGTLC YLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST
Sbjct: 70  FIRVALVLIMYLGIGTLCVYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST 129

Query: 121 KLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNKARNK 180
           KLLACAF+FTGMAVVGLILTNAADYLVEKQEIFLFK FHMHQNGSC  TE+IDTNKARNK
Sbjct: 130 KLLACAFMFTGMAVVGLILTNAADYLVEKQEIFLFKAFHMHQNGSCGRTEKIDTNKARNK 189

Query: 181 CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW 240
           CIVVFLFLLLFII GTVFLVTLE+LDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW
Sbjct: 190 CIVVFLFLLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW 249

Query: 241 ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY 300
           ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY
Sbjct: 250 ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY 309

Query: 301 KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLARSS 342
           KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLA+SS
Sbjct: 310 KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQSS 351

BLAST of Csor.00g036810 vs. ExPASy TrEMBL
Match: A0A6J1FCF3 (two-pore potassium channel 1 OS=Cucurbita moschata OX=3662 GN=LOC111442740 PE=3 SV=1)

HSP 1 Score: 640 bits (1652), Expect = 3.78e-231
Identity = 333/342 (97.37%), Postives = 335/342 (97.95%), Query Frame = 0

Query: 1   MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPS 60
           MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHS SPHTETT T TGLVPRSGLVFGNLHPS
Sbjct: 10  MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSGSPHTETTSTATGLVPRSGLVFGNLHPS 69

Query: 61  FIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST 120
           FIRVALVLIMYLGIGTLCFYLV HQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST
Sbjct: 70  FIRVALVLIMYLGIGTLCFYLVGHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST 129

Query: 121 KLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNKARNK 180
           KLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFK FHMHQNG  DIT+EIDTNKARNK
Sbjct: 130 KLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKAFHMHQNGPFDITKEIDTNKARNK 189

Query: 181 CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW 240
           CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW
Sbjct: 190 CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW 249

Query: 241 ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY 300
           ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY
Sbjct: 250 ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY 309

Query: 301 KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLARSS 342
           KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLA+SS
Sbjct: 310 KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQSS 351

BLAST of Csor.00g036810 vs. ExPASy TrEMBL
Match: A0A6J1IFC3 (two-pore potassium channel 1-like OS=Cucurbita maxima OX=3661 GN=LOC111476430 PE=3 SV=1)

HSP 1 Score: 635 bits (1639), Expect = 3.62e-229
Identity = 330/342 (96.49%), Postives = 334/342 (97.66%), Query Frame = 0

Query: 1   MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLVFGNLHPS 60
           MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHS SPHTETT T TGLVPRSGLVFGNLHPS
Sbjct: 10  MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSGSPHTETTSTATGLVPRSGLVFGNLHPS 69

Query: 61  FIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST 120
           FIRVALVLIMYLGIGTLC YLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST
Sbjct: 70  FIRVALVLIMYLGIGTLCVYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPST 129

Query: 121 KLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNKARNK 180
           KLLACAF+FTGMAVVGLILTNAADYLVEKQEIFLFK FHMHQNGSC  TE+IDTNKARNK
Sbjct: 130 KLLACAFMFTGMAVVGLILTNAADYLVEKQEIFLFKAFHMHQNGSCGRTEKIDTNKARNK 189

Query: 181 CIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW 240
           CIVVFLFLLLFII GTVFLVTLE+LDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW
Sbjct: 190 CIVVFLFLLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFW 249

Query: 241 ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY 300
           ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY
Sbjct: 250 ILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFVIY 309

Query: 301 KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLARSS 342
           KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLA+SS
Sbjct: 310 KLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQSS 351

BLAST of Csor.00g036810 vs. ExPASy TrEMBL
Match: A0A5D3C7V3 (Two-pore potassium channel 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G00580 PE=3 SV=1)

HSP 1 Score: 570 bits (1468), Expect = 4.55e-203
Identity = 295/344 (85.76%), Postives = 320/344 (93.02%), Query Frame = 0

Query: 1   MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGT----VTGLVPRSGLVFGN 60
           +LPTSSNT  TR IDIPRSKRRLRRTKSAPH++SP TE T T     TG VPRSGL+FGN
Sbjct: 10  LLPTSSNTLETRVIDIPRSKRRLRRTKSAPHANSP-TEITRTSNVPATGPVPRSGLIFGN 69

Query: 61  LHPSFIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPN 120
           LHPSF RVALVLI+YLGIGTLCFYLVR+QIKG KTNG+VDAIYFTIVTMTTVGYGDLVPN
Sbjct: 70  LHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPN 129

Query: 121 TPSTKLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNK 180
           +PSTKLLACAFVF+GMA+VGLIL+NAADYLVEKQEI LFK FH+HQNG CDI++EIDTNK
Sbjct: 130 SPSTKLLACAFVFSGMALVGLILSNAADYLVEKQEILLFKAFHIHQNGHCDISKEIDTNK 189

Query: 181 ARNKCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLF 240
           ARNKC+VVFL LLLFII GT FLV +E+LDFIDAFYCVCSTITTLGYGD+SFST+WGR+F
Sbjct: 190 ARNKCVVVFLLLLLFIISGTAFLVIIEKLDFIDAFYCVCSTITTLGYGDQSFSTKWGRIF 249

Query: 241 AIFWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAE 300
           AIFWILIS+ITLAQFFLYIA LNTERR+KSLVKWVLSKKVT +DLE AD+DDDGVVGAAE
Sbjct: 250 AIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADIDDDGVVGAAE 309

Query: 301 FVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAR 340
           FVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLA+
Sbjct: 310 FVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQ 352

BLAST of Csor.00g036810 vs. ExPASy TrEMBL
Match: A0A1S3CI53 (two-pore potassium channel 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501240 PE=3 SV=1)

HSP 1 Score: 566 bits (1460), Expect = 7.51e-202
Identity = 293/344 (85.17%), Postives = 320/344 (93.02%), Query Frame = 0

Query: 1   MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGT----VTGLVPRSGLVFGN 60
           +LPTSSNT  TR IDIPRSKRRLRRTKSAPH++SP TE T T     TG VPRSGL+FGN
Sbjct: 10  LLPTSSNTLETRVIDIPRSKRRLRRTKSAPHANSP-TEITRTSNVPATGPVPRSGLIFGN 69

Query: 61  LHPSFIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPN 120
           LHPSF RVALVLI+YLGIGTLCFYLVR+QIKG K+NG+VDAIYFTIVTMTTVGYGDLVPN
Sbjct: 70  LHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKSNGIVDAIYFTIVTMTTVGYGDLVPN 129

Query: 121 TPSTKLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNK 180
           +PSTKLLACAFVF+GMA+VGLIL+NAADYLVEKQEI LFK FH+HQNG CDI++EIDTNK
Sbjct: 130 SPSTKLLACAFVFSGMALVGLILSNAADYLVEKQEILLFKAFHIHQNGHCDISKEIDTNK 189

Query: 181 ARNKCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLF 240
           ARNKC+VVFL LLLFII GT FLV +E+LDFIDAFYCVCSTITTLGYGD+SFST+WGR+F
Sbjct: 190 ARNKCVVVFLLLLLFIISGTAFLVIIEKLDFIDAFYCVCSTITTLGYGDQSFSTKWGRIF 249

Query: 241 AIFWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAE 300
           AIFWILIS+ITLAQFFLYIA LNTERR+KSLVKWVLS+KVT +DLE AD+DDDGVVGAAE
Sbjct: 250 AIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSEKVTDIDLEVADIDDDGVVGAAE 309

Query: 301 FVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAR 340
           FVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLA+
Sbjct: 310 FVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQ 352

BLAST of Csor.00g036810 vs. ExPASy TrEMBL
Match: A0A0A0K3G0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G098760 PE=3 SV=1)

HSP 1 Score: 558 bits (1439), Expect = 1.19e-198
Identity = 293/344 (85.17%), Postives = 317/344 (92.15%), Query Frame = 0

Query: 1   MLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGT----VTGLVPRSGLVFGN 60
           +LPTSSNT  TR I+IPRSKRRLRRTKSAPH++SP TE T T     TG VPRSGL+FGN
Sbjct: 10  LLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSP-TEITHTSNVPATGPVPRSGLIFGN 69

Query: 61  LHPSFIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPN 120
           LHPSF RVALVLI YLGIGTLCFYLVRHQI+G KTN +VDAIYFTIVTMTTVGYGDLVPN
Sbjct: 70  LHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTTVGYGDLVPN 129

Query: 121 TPSTKLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNK 180
           +PSTKLLACAFVFTGMA+VGLIL+NAADYLVEKQEI LFK FH+ QNG CDI++EIDTNK
Sbjct: 130 SPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQNGHCDISKEIDTNK 189

Query: 181 ARNKCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLF 240
           ARNKCIVVFL LLLFII GT FLVT+E+LDFIDAFYCVCSTITTLGYGD+SFST+WGR+F
Sbjct: 190 ARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQSFSTKWGRVF 249

Query: 241 AIFWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAE 300
           AIFWILIS+ITLAQFFLYIA LNTERR+KSLVKWVLSKKVT +DLE AD+DDDGVVGAAE
Sbjct: 250 AIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADIDDDGVVGAAE 309

Query: 301 FVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAR 340
           FVIYKLKEMGKITEDDIS VL+EFENLDVDQSGTLSISDITLA+
Sbjct: 310 FVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQ 352

BLAST of Csor.00g036810 vs. TAIR 10
Match: AT5G55630.1 (Outward rectifying potassium channel protein )

HSP 1 Score: 378.6 bits (971), Expect = 5.2e-105
Identity = 201/344 (58.43%), Postives = 258/344 (75.00%), Query Frame = 0

Query: 2   LPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLV--FGNLHP 61
           + T +  F   S      KRRLRR++SAP  D  + +    +    P    +  F +L+P
Sbjct: 16  IDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDV-KIDEPPPHPSKIPMFSDLNP 75

Query: 62  SFIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPS 121
           +  RV + L +YL IGTLCFYLVR QI G KT+GVVDA+YF IVTMTTVGYGDLVPN+ +
Sbjct: 76  NLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGDLVPNSSA 135

Query: 122 TKLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQN-GSCDITEEIDTNKAR 181
           ++LLACAFVF+GM +VG +L+ AADYLVEKQE  L + FH+ Q+ G  DI +E+ TNK R
Sbjct: 136 SRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQSFGPTDILKELHTNKLR 195

Query: 182 NKCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAI 241
            KC    L L++  I GT+FLV +E++  I AFYCVCST+TTLGYGDKSF++  GRLFA+
Sbjct: 196 YKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTTLGYGDKSFNSEAGRLFAV 255

Query: 242 FWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFV 301
           FWIL SSI LAQFFLY+A LNTE ++++LVKWVL++++T  DLEAADLD+DGVVGAAEF+
Sbjct: 256 FWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLDEDGVVGAAEFI 315

Query: 302 IYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLARSS 343
           +YKLKEMGKI E DIS ++DEFE LD D+SGTL+ SDI LA+++
Sbjct: 316 VYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIVLAQTT 358

BLAST of Csor.00g036810 vs. TAIR 10
Match: AT5G55630.2 (Outward rectifying potassium channel protein )

HSP 1 Score: 378.6 bits (971), Expect = 5.2e-105
Identity = 201/344 (58.43%), Postives = 258/344 (75.00%), Query Frame = 0

Query: 2   LPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGTVTGLVPRSGLV--FGNLHP 61
           + T +  F   S      KRRLRR++SAP  D  + +    +    P    +  F +L+P
Sbjct: 16  IDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDV-KIDEPPPHPSKIPMFSDLNP 75

Query: 62  SFIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPS 121
           +  RV + L +YL IGTLCFYLVR QI G KT+GVVDA+YF IVTMTTVGYGDLVPN+ +
Sbjct: 76  NLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGDLVPNSSA 135

Query: 122 TKLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQN-GSCDITEEIDTNKAR 181
           ++LLACAFVF+GM +VG +L+ AADYLVEKQE  L + FH+ Q+ G  DI +E+ TNK R
Sbjct: 136 SRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQSFGPTDILKELHTNKLR 195

Query: 182 NKCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAI 241
            KC    L L++  I GT+FLV +E++  I AFYCVCST+TTLGYGDKSF++  GRLFA+
Sbjct: 196 YKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTTLGYGDKSFNSEAGRLFAV 255

Query: 242 FWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAAEFV 301
           FWIL SSI LAQFFLY+A LNTE ++++LVKWVL++++T  DLEAADLD+DGVVGAAEF+
Sbjct: 256 FWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLDEDGVVGAAEFI 315

Query: 302 IYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLARSS 343
           +YKLKEMGKI E DIS ++DEFE LD D+SGTL+ SDI LA+++
Sbjct: 316 VYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIVLAQTT 358

BLAST of Csor.00g036810 vs. TAIR 10
Match: AT4G01840.1 (Ca2+ activated outward rectifying K+ channel 5 )

HSP 1 Score: 184.9 bits (468), Expect = 1.1e-46
Identity = 110/289 (38.06%), Postives = 172/289 (59.52%), Query Frame = 0

Query: 60  SFIRVAL-VLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTP 119
           S IR A+ +LI+YL +G   +   R    G +T+ VVDA+YF IVTM T+GYGD+ P TP
Sbjct: 115 SIIRQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTP 174

Query: 120 STKLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLF---------KVFHMHQNGSCD-- 179
            TK+ A  FV  G   + ++L+   +Y+++ QE  +          +  H H+  + D  
Sbjct: 175 WTKIFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYI 234

Query: 180 ITEEIDTNKARNKCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKS 239
           I  E    + R K  +    ++L I  G + L  +EEL F+D+ Y    ++TT+GYGD++
Sbjct: 235 IDFEKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRA 294

Query: 240 FSTRWGRLFAIFWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLD 299
           F T  GRLFA  W+L+S++ +A+ FLY+A    +RR +  VK  L++++TV DL  AD  
Sbjct: 295 FKTLQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTY 354

Query: 300 DDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDI 337
             G +  +E+++ KLKEMGKIT+ DI  V+ +FE LD +Q G +++ D+
Sbjct: 355 QHGFISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPDL 403

BLAST of Csor.00g036810 vs. TAIR 10
Match: AT4G18160.1 (Ca2+ activated outward rectifying K+ channel 6 )

HSP 1 Score: 181.4 bits (459), Expect = 1.2e-45
Identity = 103/282 (36.52%), Postives = 167/282 (59.22%), Query Frame = 0

Query: 60  SFIRVAL-VLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTP 119
           S +R A  +L++YL +G L ++L R      +T+ VVD +YF IVTM T+GYGD+ PN+ 
Sbjct: 148 SVVRQAFALLVVYLSLGVLIYWLNRDHYVVNQTHPVVDGLYFCIVTMCTIGYGDITPNSV 207

Query: 120 STKLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEEIDTNKAR 179
            TKL +  FV  G   + ++L+    Y+++ QE ++              +  ID  K R
Sbjct: 208 VTKLFSIMFVLVGFGFIDILLSGMVSYVLDLQESYMLDSAKRRDEPEKRRSYIIDVKKGR 267

Query: 180 N----KCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGR 239
                K  +    ++L I  G   +  +EE+ ++D+FY    ++TT+GYGD++F T  GR
Sbjct: 268 MRIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLDSFYLSVMSVTTVGYGDRAFKTLPGR 327

Query: 240 LFAIFWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGA 299
           LFA  W+L+S++ +A+ FLY+A    ++R +   K VL + ++V    AAD+D++G V  
Sbjct: 328 LFAAIWLLVSTLAVARAFLYLAEARVDKRNRERAKKVLCETMSVSQFFAADIDNNGCVSK 387

Query: 300 AEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDI 337
           AE+VIYKLKEM KIT+ DI P+  +F+ LD   +G +++ D+
Sbjct: 388 AEYVIYKLKEMEKITDKDILPISKQFDKLDRCSNGKITLLDL 429

BLAST of Csor.00g036810 vs. TAIR 10
Match: AT5G46370.1 (Ca2+ activated outward rectifying K+ channel 2 )

HSP 1 Score: 171.8 bits (434), Expect = 9.6e-43
Identity = 98/281 (34.88%), Postives = 170/281 (60.50%), Query Frame = 0

Query: 67  VLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTTVGYGDLVPNTPSTKLLACA 126
           +L++YL +G L ++L R      +T+ VVDA+YF IVTM T+GYGD+ P++  TKL +  
Sbjct: 152 LLVVYLSLGVLIYWLNRDSYNVKQTHPVVDALYFCIVTMCTIGYGDITPDSVVTKLFSIF 211

Query: 127 FVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCDITEE-------IDTNKARN 186
           FV  G   + ++L+    Y+++ QE ++ +     +N S ++ +        ID  K R 
Sbjct: 212 FVLVGFGFMDILLSGMVTYVLDLQENYMLET---ARNESLNLNDRDKVRSYIIDVKKGRM 271

Query: 187 ----KCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKSFSTRWGRL 246
               K  +    ++L + FG + +  +E++ ++D+FY    ++TT+GYGD++F+T  GRL
Sbjct: 272 RIRLKVGLALGVVVLCLGFGVLIMHFVEKIGWLDSFYFSVMSVTTVGYGDRAFNTLAGRL 331

Query: 247 FAIFWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLDDDGVVGAA 306
            A  W+L+S++ +A+  L++A    ++R +   K VL + +++     AD+D +G V  A
Sbjct: 332 LAAMWLLVSTLAVARAILFLAESRVDKRNRERAKKVLGESMSISQFLDADIDCNGCVSKA 391

Query: 307 EFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDI 337
           EFVIYKLK+M KITE DI+P+  +F+ LD   SG +++ D+
Sbjct: 392 EFVIYKLKKMDKITEKDINPIGFQFDKLDRTNSGRITLLDL 429

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LBL17.3e-10458.43Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2[more]
Q850M01.2e-9055.59Two pore potassium channel a OS=Oryza sativa subsp. japonica OX=39947 GN=TPKA PE... [more]
Q8LIN56.4e-8451.88Two pore potassium channel b OS=Oryza sativa subsp. japonica OX=39947 GN=TPKB PE... [more]
Q9S6Z81.5e-4538.06Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1[more]
Q9SVV61.7e-4436.52Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6592092.16.63e-239100.00Two-pore potassium channel 1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7024969.13.78e-23899.71Two-pore potassium channel 1, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_023535214.15.14e-23698.54two-pore potassium channel 1 [Cucurbita pepo subsp. pepo][more]
XP_022936020.17.81e-23197.37two-pore potassium channel 1 [Cucurbita moschata][more]
XP_022975856.17.48e-22996.49two-pore potassium channel 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1FCF33.78e-23197.37two-pore potassium channel 1 OS=Cucurbita moschata OX=3662 GN=LOC111442740 PE=3 ... [more]
A0A6J1IFC33.62e-22996.49two-pore potassium channel 1-like OS=Cucurbita maxima OX=3661 GN=LOC111476430 PE... [more]
A0A5D3C7V34.55e-20385.76Two-pore potassium channel 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3CI537.51e-20285.17two-pore potassium channel 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501240 ... [more]
A0A0A0K3G01.19e-19885.17Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G098760 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G55630.15.2e-10558.43Outward rectifying potassium channel protein [more]
AT5G55630.25.2e-10558.43Outward rectifying potassium channel protein [more]
AT4G01840.11.1e-4638.06Ca2+ activated outward rectifying K+ channel 5 [more]
AT4G18160.11.2e-4536.52Ca2+ activated outward rectifying K+ channel 6 [more]
AT5G46370.19.6e-4334.88Ca2+ activated outward rectifying K+ channel 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003280Two pore domain potassium channelPRINTSPR013332POREKCHANELcoord: 103..131
score: 31.64
coord: 219..228
score: 45.91
IPR003280Two pore domain potassium channelPANTHERPTHR11003POTASSIUM CHANNEL, SUBFAMILY Kcoord: 16..334
IPR013099Potassium channel domainPFAMPF07885Ion_trans_2coord: 67..147
e-value: 2.6E-16
score: 59.4
coord: 187..257
e-value: 2.0E-14
score: 53.3
NoneNo IPR availableGENE3D1.10.287.70coord: 58..157
e-value: 2.4E-21
score: 77.4
NoneNo IPR availableGENE3D1.10.287.70coord: 165..271
e-value: 1.2E-19
score: 72.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 20..43
NoneNo IPR availablePANTHERPTHR11003:SF271OUTWARD RECTIFYING POTASSIUM CHANNEL PROTEINcoord: 16..334
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 58..162
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 174..280
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 285..297
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 277..337

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g036810.m01Csor.00g036810.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030007 cellular potassium ion homeostasis
biological_process GO:0071257 cellular response to electrical stimulus
biological_process GO:0097623 potassium ion export across plasma membrane
biological_process GO:1990573 potassium ion import across plasma membrane
biological_process GO:0010029 regulation of seed germination
biological_process GO:0010119 regulation of stomatal movement
biological_process GO:0030322 stabilization of membrane potential
biological_process GO:0071805 potassium ion transmembrane transport
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022841 potassium ion leak channel activity
molecular_function GO:0005267 potassium channel activity