Csor.00g032890 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g032890
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionLipid droplet-associated hydrolase
LocationCsor_Chr07: 3928935 .. 3932821 (+)
RNA-Seq ExpressionCsor.00g032890
SyntenyCsor.00g032890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTCAAGAAGTTCTGAATCCTTTTAGTAGAAGGCGCGCGCAGTTTCGTTTGTGCAATGTATCAGGGTATTCGATTCTCGTTTATTTTCATTGAAATTTCTCTTGGAATTGTGGTTTTCCATGAATTAAATCGCCTTTCGTTTCTGCGTTTTGATAGTTACACTAATGAATTGCTGGAGATTCATGCGGATGATCCATCGTTGCACGTATTGTTCATACCTGGAAATCCAGGTCCATATGCTTTTGTGGATTTGGGATTTCGATGCTTCTTTTGATTTTTGCATAATTTCTGAATGGGTTTTGTTCTAAGACAGGGATTGTTTCGTTTTATAAAGATTTTGTTGAATCCCTGTACCAGTTGCTTGGGGGCCGTGTATCTATTACAGGCAAGACATGAATTGTTGGCATTGTTCTTATAGAAAACATTAAATCTTTTACATCTGGGCTGATACTTTTTCTCTTTTTGGCCATCCTTTTGTATACTATCAGCTATTGGTCATCTATCTCAAACGACGAAGGTACTTGCACATCGGTCGTATAAAGTTCATATCTGCTATGCATTTTAGCTTTGGCGAGTGACAAATTCTTTTGGAAATGATGTTTTGTGCACTGCATTCTTATATATGAAATATCAATGGCTCTATGTAGTTGGAGGCTCTGGTTAGTTATGCTTAAGTTTAATGCTTCAAAAGGAAAATTAGTTTATGTTGCTCGGATGCATATAGTGAAGTTTGTTACATAAATTTCCCCAGTGATTTTATGTAATGGATATTGATGTTTCTTTTCTTTTAGGATTGGGAAGGTGGAAGGTTATTTTCATTACAAGAACAAATTGATCATAAGGTAATTCTCTGTTTTATCCGATTTGATTTAGTTATTGATAGCCTAGGGTGGCAAGTGGTCCATGAAAAATATGAAGTCGCGAGTGTACGTCATTTTAAATTGCATGGAGAGCTGTGCATGTGAAATCCCACGTCGGTTGGAGATGGGAGCAGAACATTCCTTATAAGGGTGTGAAAACCTCTCCCTAGCAGATGCGATTTATAACCCTGAGGCTGATGACGATACGTAACGGGCCAAAGCGAACAATATCTGCTAGTGGTGGGCTTGAGTTGTTACAAATGGTACTAGAGCTGGACACCGAGCGGTGTGCCAGCAAGGACGTTGGGCCCCCAATGGGGGTGGAGATTGTGTGATCCCATATCGGTTGGAGAGGGGAACAAAACATTCCTTATAAGGGCGTGGAAACCTCTCCCTAGCAGACGCATTTTAAACCCGTGAGGCTGATAGCGATACGTAACGGGCCAAAGCGGACAATATCTGCTAGCGGTGGGCTTGAGCTGTTACAGTTCAATTGGAATAACATTGCAGAAATTTGGAATTAATATCTCTTGCTCAAGATTATTGTTCATTTCTTTATTATTGTTCATTTCTTTATTATCTGTTTCCTACAGGTCTCTTTTCTCTTTCATATATGGGGCTGAACTATATTCATATTTCTTTTGCAGATAGAATTTGTCAGACAAGAATTACAGAATAAAGACATTCCATTGCTACTGGTAGTTATACCTTGATGATTACAGTTTATGCTCAATGTTCACGATAATCATAAAAGAGTCGCACGGTGCAAAATTTTCAATTGTATTGTTTTGATATCATCTGAATACAATTGAGTTATTCTTTTATTAGTAAGCCACTGCAACCCTTATAAAATTTTATTCTAGTTTAACCCATACACAATAGTTTCTAGTTATAGTGCTCATATTCTTCTTGTTACCTGTTAACTAAACCGAGACAATTTCACTTGTTATGTTAAGAATGAACTTGTTACAATTTTGCAAAGTTCTTCGTTATGACTGTTATTTCAGTAGCTGACCCGTTTGTTCTCTTTGAAGGTTGGGCATTCTATTGGATCGTATATCTCGATTGAATTGTACCGCAAGTTCCAAGATCAGGTATTGGCATAAACTCTTTCTGCTTCTTATGTATTCCCAGCTTTCAAGAGATGTTGCAATGGAAATTTTCCTTGAACCCATAGGTGGTGTATTGTATTGGATTGCATCCATTTATGATGGTGAACAGACAATCTCGTCAGCAATTTTTAATTGGAAAACTTGCAGGGTATGCCCGTTATTTGTTCTGATAACATGAATTATGCCTTCCTGCATCTACATTGCCATCAAAGTCTCTTCACTCTTTCTAATTATAGGACCCTTTTAAAATCTTTCCCACTTCAGTGGATTCAAACTCAGCCTTAGGGAGTTTCTCAGTTTTGTGTATCTTTTATATTGTTTTTGTGAGATCCCACATCGGTTGGGGAGAAGAACGAAGCATTCTTAACAAGGGTGTGGAAACCTCTCCTTAGTAGACGCATTTTAAAAACCTTGAAGGGAAGCCAAAGATTAATAGTACACTGTAAGGCTGACAAGATAATTTTGTGCTGTAGATCCCGTCTTCTATCAACTTTGTTTTCATCTTTTGTCGCACTGTTGGGTGTATTACCGAGCCGAGTTTCAAGTTTTGTTGTGAGTAAAACTATTGGGAAGTCATGGTCCAGAACTGCAAGTGAGGCTGCTTGCACTTATTTGCTAAAGGTGAAATGAAATCTAATGTATTATTTGTAGTATATGCATTTTTAGTATCCTCTTGAAACTGCTATAAATTTTGATTCTAGGATAATGACTGTCTGTTCTGATATTTGGAATATCATCTTTGTGTTCTACAGTACCATGTCATGCGAAATATTCTATTTATGGCGTTGACAGAGTTCGAGAAGGTATTCACTATGAAAAATCGCGATAGTTAGGCAAACGTGTCGCTCATATCAGACATTTGTATCACTGAAATCTTCGTTTTCGTGGAAGTACACAGCGTAATATCAATATTTAGTCAAATTTCCTCATCTGATTGTGTATGGACATTGTTTAGCTTTCAGAAACACCAGATTGGGCATTCATGAAGAAATCAAGTCGGAAATTATCATTCTTGTTCTGTATGGATGACCACTGGGCTCCAATGCATGTATTTGAGGAGGTAGGACATCGAACCACTTTTCCATTTTATTAATCATTTCTGCTGCATGCATGTAGCTTCGGCGAATATTGTTGTTATGGAGTCTACTGCTTATTTATAACTTAGTCAATGGTTATACATGGTAAAAGTATATATGGTAAATGACATAAACAACTATGTATAGTAAAAAGCTATATATGTTAAAGCAGTTATCCCATTAGGTAGTGCTTTGAAAATGTACTTTCTGCATTCTCCGTATTCTATACTACTCTACATGTATGAAGTCGTCAATATAAACATTCAGCCAATATTCCTTATTACATTTTCTCTCTCCTATTCTTTGTGTTCATCTAGATCGAGTATGCATTGTTGTGCGATTCTAATAATTAGAAAGTACAACGATAGTCGGTTTGAAATATTATTAACTTCGTATTATGCATGGTGAACAACTTCAAATAGCAGGATTGAAAAAGTAATAATTTGAAGCTTAAAACTGTAACTGATGTAGTGAATTCAAGTTCTAGTGTTGTTCTTGTTTGAGTTTTGTTTTCTCCACAGATATCGAAGCAGATCCCGGAAATGGATGTATCAGTTGAAAGGGAAGGTCATACACATGCATTTTGCTGCTCTGAAGCTGGCTCACTCTATATTGCTCAACATGTGGCAAGCTTGATCAAGAATCACATCTCAGATTAAGGTCAGATTCATTCATCATTCCTTCCTTCTTACTCCATTGTAGAGACTTCCTTTGTGTTTTATATCGGATGTTCGTTCTTAACGTTTTGTAGTAGCAGCGCATGCTTTTCGACAATGAACGTCTCCCCATACACTATCATTCCTATAGAAACCTCATGTCGTCGAACAGGAAAATAG

mRNA sequence

ATGGGTCAAGAAGTTCTGAATCCTTTTAGTAGAAGGCGCGCGCAGTTTCGTTTGTGCAATGTATCAGGTTACACTAATGAATTGCTGGAGATTCATGCGGATGATCCATCGTTGCACGTATTGTTCATACCTGGAAATCCAGGGATTGTTTCGTTTTATAAAGATTTTGTTGAATCCCTGTACCAGTTGCTTGGGGGCCGTGTATCTATTACAGCTATTGGTCATCTATCTCAAACGACGAAGGATTGGGAAGGTGGAAGGTTATTTTCATTACAAGAACAAATTGATCATAAGATAGAATTTGTCAGACAAGAATTACAGAATAAAGACATTCCATTGCTACTGGTTGGGCATTCTATTGGATCGTATATCTCGATTGAATTGTACCGCAAGTTCCAAGATCAGGTGGTGTATTGTATTGGATTGCATCCATTTATGATGGTGAACAGACAATCTCGTCAGCAATTTTTAATTGGAAAACTTGCAGGATCCCGTCTTCTATCAACTTTGTTTTCATCTTTTGTCGCACTGTTGGGTGTATTACCGAGCCGAGTTTCAAGTTTTGTTGTGAGTAAAACTATTGGGAAGTCATGGTCCAGAACTGCAAGTGAGGCTGCTTGCACTTATTTGCTAAAGTACCATGTCATGCGAAATATTCTATTTATGGCGTTGACAGAGTTCGAGAAGCTTTCAGAAACACCAGATTGGGCATTCATGAAGAAATCAAGTCGGAAATTATCATTCTTGTTCTGTATGGATGACCACTGGGCTCCAATGCATGTATTTGAGGAGATATCGAAGCAGATCCCGGAAATGGATGTATCAGTTGAAAGGGAAGAATCACATCTCAGATTAAGTAGCAGCGCATGCTTTTCGACAATGAACGTCTCCCCATACACTATCATTCCTATAGAAACCTCATGTCGTCGAACAGGAAAATAG

Coding sequence (CDS)

ATGGGTCAAGAAGTTCTGAATCCTTTTAGTAGAAGGCGCGCGCAGTTTCGTTTGTGCAATGTATCAGGTTACACTAATGAATTGCTGGAGATTCATGCGGATGATCCATCGTTGCACGTATTGTTCATACCTGGAAATCCAGGGATTGTTTCGTTTTATAAAGATTTTGTTGAATCCCTGTACCAGTTGCTTGGGGGCCGTGTATCTATTACAGCTATTGGTCATCTATCTCAAACGACGAAGGATTGGGAAGGTGGAAGGTTATTTTCATTACAAGAACAAATTGATCATAAGATAGAATTTGTCAGACAAGAATTACAGAATAAAGACATTCCATTGCTACTGGTTGGGCATTCTATTGGATCGTATATCTCGATTGAATTGTACCGCAAGTTCCAAGATCAGGTGGTGTATTGTATTGGATTGCATCCATTTATGATGGTGAACAGACAATCTCGTCAGCAATTTTTAATTGGAAAACTTGCAGGATCCCGTCTTCTATCAACTTTGTTTTCATCTTTTGTCGCACTGTTGGGTGTATTACCGAGCCGAGTTTCAAGTTTTGTTGTGAGTAAAACTATTGGGAAGTCATGGTCCAGAACTGCAAGTGAGGCTGCTTGCACTTATTTGCTAAAGTACCATGTCATGCGAAATATTCTATTTATGGCGTTGACAGAGTTCGAGAAGCTTTCAGAAACACCAGATTGGGCATTCATGAAGAAATCAAGTCGGAAATTATCATTCTTGTTCTGTATGGATGACCACTGGGCTCCAATGCATGTATTTGAGGAGATATCGAAGCAGATCCCGGAAATGGATGTATCAGTTGAAAGGGAAGAATCACATCTCAGATTAAGTAGCAGCGCATGCTTTTCGACAATGAACGTCTCCCCATACACTATCATTCCTATAGAAACCTCATGTCGTCGAACAGGAAAATAG

Protein sequence

MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESLYQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSIGSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGVLPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMKKSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVEREESHLRLSSSACFSTMNVSPYTIIPIETSCRRTGK
Homology
BLAST of Csor.00g032890 vs. ExPASy Swiss-Prot
Match: Q5F477 (Lipid droplet-associated hydrolase OS=Gallus gallus OX=9031 GN=LDAH PE=2 SV=2)

HSP 1 Score: 104.8 bits (260), Expect = 1.9e-21
Identity = 75/254 (29.53%), Postives = 127/254 (50.00%), Query Frame = 0

Query: 36  PSLHVLFIPGNPGIVSFYKDFVESLYQLLGGRVSITAIGH-----------LSQTTKDWE 95
           P L  + IPGNPG+  +Y+ F+++LY  L  +  +  + H           + + T   E
Sbjct: 43  PRLLFMIIPGNPGLAGYYRTFIKALYCGLNQQYPVWVVSHAGHCKPPSGMEMIEDTDIKE 102

Query: 96  GGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSIGSYISIELYRKFQD-QVVYCIGLH 155
              +F L  Q++HK+ F+++ + +KDI L+L+ HSIG YI++E+ ++  + QV+  + L 
Sbjct: 103 LEDVFGLNGQVEHKLNFLKKNV-SKDIKLVLIAHSIGCYITLEMMKRASELQVLRSVLLF 162

Query: 156 PFMMVNRQSRQQFLIGKLAGSRLLSTLFSSF--VALLGVLPSRVSSFVVSKTI-GKSWSR 215
           P +    QS Q    GKL    L    ++ +  V LL  LP  V + +V   + G     
Sbjct: 163 PTIERMAQSPQ----GKLMTPLLCKLRYALYMPVYLLSFLPEGVKASLVRFALRGMKTCD 222

Query: 216 TASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMKKSSRKLSFLFCMDDHWAPMH 275
            +S      L     + NIL+MA  E  K+ E  D   +K++ +KL F +   D W P +
Sbjct: 223 ESSITTSVNLFSVDCIANILYMASQEMMKVVER-DSTTIKQNLKKLIFYYGTGDSWCPQN 282

BLAST of Csor.00g032890 vs. ExPASy Swiss-Prot
Match: Q54LL8 (Lipid droplet-associated hydrolase OS=Dictyostelium discoideum OX=44689 GN=DDB_G0286581 PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 3.5e-20
Identity = 81/269 (30.11%), Postives = 130/269 (48.33%), Query Frame = 0

Query: 27  ELLEIHADDPS-LHVLFIPGNPGIVSFYKDFVESLYQLLGGRVSITAIGHLSQTTKDWEG 86
           E++   +  PS + ++ I GNPGI SFY++FV+ L      +  I  +GH+    K    
Sbjct: 21  EIIYTKSQTPSNIKIIVIAGNPGIESFYQEFVKVLNLSFNSKYDIYGVGHIGHCGK--IE 80

Query: 87  GRLFSLQEQIDHKIEFVRQELQN-------KDIPLLLVGHSIGSYISIELYRKFQDQVVY 146
            + FS++EQI HK  F+   L+N       KDI  +L+GHS+GSYIS+++  +F ++  +
Sbjct: 81  NKTFSVEEQIKHKELFLEYLLKNKYGDKDRKDIKFILIGHSVGSYISLKVVSRFSEKFEF 140

Query: 147 CI-------------GLHPF--MMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGVLPS 206
                          GL PF  M+V R+S +  L              S+F   L  +PS
Sbjct: 141 LSVVNLFPTFKNLYDGLSPFIKMVVMRESTRNGL--------------STF---LHYIPS 200

Query: 207 RVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMKKSS 266
            V S V+   +    SR A ++     + Y+   NIL+MA TE E + E  D      +S
Sbjct: 201 IVVSNVLKWILPSDESRIAVQSK----INYYSALNILYMAYTETEDIKEIDDECHSVFNS 260

Query: 267 R--KLSFLFCMDDHWAPMHVFEEISKQIP 271
           R  +L F++   D + P   ++E+ +  P
Sbjct: 261 RLNQLLFIYGQTDSYTPKSFYDEMKQLYP 266

BLAST of Csor.00g032890 vs. ExPASy Swiss-Prot
Match: Q5HZX7 (Lipid droplet-associated hydrolase OS=Rattus norvegicus OX=10116 GN=Ldah PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.1e-18
Identity = 73/257 (28.40%), Postives = 124/257 (48.25%), Query Frame = 0

Query: 39  HVLF-IPGNPGIVSFYKDFVESLYQLLGGRVSITAIGH--LSQTTKD------------W 98
           H++F IPGNPG+  FY  F ++LY L+ G   +  I H     T KD             
Sbjct: 45  HLIFVIPGNPGLSPFYVPFAKALYSLVKGHFPVWIISHAGFCLTPKDKKILTAPQEPNAQ 104

Query: 99  EGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSIGSYISIELYRKFQDQVVYCIGLH 158
           E   ++ L  QI+HKI F+R  +  KD+ L+ +GHS+GSYI++ +  +  +  V    +H
Sbjct: 105 EIEDIYGLNGQIEHKIAFLRAHVP-KDVKLIFIGHSVGSYIALHVMNRAPELPV----VH 164

Query: 159 PFMM---VNRQSRQQFLIGKLAG---SRLLSTLFSSFVALLGVLPSRVSSFVVSKTIGKS 218
            F++   + R S      GK A     R    L+++   +    P  + SF+V K + K 
Sbjct: 165 TFLLFPTIERMSESP--NGKFATPFLCRFRYMLYAASYLIFKPCPEMIKSFIVQKVLEKM 224

Query: 219 WSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMKKSSRKLSFLFCMDDHWA 275
             +  SE   T LL+   + N  ++   E   + +  D   +K+   KL+F +   D W 
Sbjct: 225 SFK--SELRLTDLLQPFCLANAAYLGGQEMIHVVKRDD-GIIKELLPKLTFYYGKTDGWC 284

BLAST of Csor.00g032890 vs. ExPASy Swiss-Prot
Match: Q8BVA5 (Lipid droplet-associated hydrolase OS=Mus musculus OX=10090 GN=Ldah PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 3.3e-18
Identity = 72/272 (26.47%), Postives = 127/272 (46.69%), Query Frame = 0

Query: 24  YTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESLYQLLGGRVSITAIGH--LSQTTK 83
           +TN   +     P   +  IPGNPG  +FY  F ++LY L+  R  +  I H   S T K
Sbjct: 31  WTNLFEKQDVSKPKQLIFIIPGNPGYSAFYVPFAKALYTLMKSRFPVWIISHAGFSVTPK 90

Query: 84  D-------------WEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSIGSYISIEL 143
           D              +   ++ L  QI+HKI F+R  +  KD+ L+L+GHS+G+Y+++ +
Sbjct: 91  DKKVLAAPQEESNAQKIEDVYGLNGQIEHKIAFLRAHVP-KDVKLILIGHSVGTYMTLHV 150

Query: 144 YRKFQDQVVYCIGLHPFMM---VNRQSRQ---QFLIGKLAGSRLLSTLFSSFVALLGVLP 203
            ++  +  V     H F++   + R S     +F    L   R L  L+++   L    P
Sbjct: 151 MKRVLELPV----AHAFLLFPTIERMSESPNGKFATPFLCQFRYL--LYATSYLLFKPCP 210

Query: 204 SRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMKKS 263
             + SF++ K +G+       E   T +L+   + N  ++   E  ++ +  D   +K+ 
Sbjct: 211 EVIKSFIIQKLMGQ--MNIKLELPLTDILQPFCLANAAYLGSQEMVQIVKRDD-DIIKEF 270

Query: 264 SRKLSFLFCMDDHWAPMHVFEEISKQIPEMDV 275
             KL F +   D W P+  +E++ K  PE ++
Sbjct: 271 LPKLKFYYGKTDGWCPVKYYEDMKKDFPEGNI 292

BLAST of Csor.00g032890 vs. ExPASy Swiss-Prot
Match: Q9H6V9 (Lipid droplet-associated hydrolase OS=Homo sapiens OX=9606 GN=LDAH PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.4e-16
Identity = 69/256 (26.95%), Postives = 118/256 (46.09%), Query Frame = 0

Query: 36  PSLHVLFIPGNPGIVSFYKDFVESLYQLLGGRVSITAIGH--------------LSQTTK 95
           P L +  IPGNPG  +FY  F ++LY L   R  +  I H               S+ + 
Sbjct: 43  PKLLIFIIPGNPGFSAFYVPFAKALYSLTNRRFPVWTISHAGHALAPKDKKILTTSEDSN 102

Query: 96  DWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSIGSYISIELYRKFQDQVVYCIG 155
             E   ++ L  QI+HK+ F+R  +  KD+ L+L+GHSIGSY ++++ ++  +  V    
Sbjct: 103 AQEIKDIYGLNGQIEHKLAFLRTHVP-KDMKLVLIGHSIGSYFTLQMLKRVPELPVIRAF 162

Query: 156 LHPFMMVNRQSRQQFLIGKLAGSRLL---STLFSSFVALLGVLPSRVSSFVVSKTIGKSW 215
           L  F  + R S      G++A   L      L+ +   LL   P  + S ++ +  G   
Sbjct: 163 L-LFPTIERMSESP--NGRIATPLLCWFRYVLYVTGYLLLKPCPETIKSLLIRR--GLQV 222

Query: 216 SRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMKKSSRKLSFLFCMDDHWAP 275
               +E +   +L+   + N  ++   E  ++ +  D   +K+   KL+F +   D W P
Sbjct: 223 MNLENEFSPLNILEPFCLANAAYLGGQEMMEVVKRDD-ETIKEHLCKLTFYYGTIDPWCP 282

BLAST of Csor.00g032890 vs. NCBI nr
Match: KAG6595161.1 (Lipid droplet-associated hydrolase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 630 bits (1626), Expect = 5.38e-228
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0

Query: 1   MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 60
           MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL
Sbjct: 1   MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 60

Query: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 120
           YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI
Sbjct: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 120

Query: 121 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 180
           GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV
Sbjct: 121 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 180

Query: 181 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK 240
           LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK
Sbjct: 181 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK 240

Query: 241 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVEREESHLRLSSSACFSTMNVSPYT 300
           KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVEREESHLRLSSSACFSTMNVSPYT
Sbjct: 241 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVEREESHLRLSSSACFSTMNVSPYT 300

Query: 301 IIPIETSCRRTGK 313
           IIPIETSCRRTGK
Sbjct: 301 IIPIETSCRRTGK 313

BLAST of Csor.00g032890 vs. NCBI nr
Match: XP_023517069.1 (lipid droplet-associated hydrolase isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 563 bits (1450), Expect = 3.11e-201
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0

Query: 1   MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 60
           MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL
Sbjct: 1   MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 60

Query: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 120
           YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI
Sbjct: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 120

Query: 121 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 180
           GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV
Sbjct: 121 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 180

Query: 181 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK 240
           LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK
Sbjct: 181 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK 240

Query: 241 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE 279
           KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE
Sbjct: 241 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE 279

BLAST of Csor.00g032890 vs. NCBI nr
Match: XP_023517065.1 (lipid droplet-associated hydrolase isoform X1 [Cucurbita pepo subsp. pepo] >XP_023517066.1 lipid droplet-associated hydrolase isoform X1 [Cucurbita pepo subsp. pepo] >XP_023517067.1 lipid droplet-associated hydrolase isoform X1 [Cucurbita pepo subsp. pepo] >XP_023517068.1 lipid droplet-associated hydrolase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 563 bits (1450), Expect = 1.41e-200
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0

Query: 1   MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 60
           MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL
Sbjct: 42  MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 101

Query: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 120
           YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI
Sbjct: 102 YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 161

Query: 121 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 180
           GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV
Sbjct: 162 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 221

Query: 181 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK 240
           LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK
Sbjct: 222 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK 281

Query: 241 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE 279
           KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE
Sbjct: 282 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE 320

BLAST of Csor.00g032890 vs. NCBI nr
Match: XP_022963432.1 (lipid droplet-associated hydrolase isoform X2 [Cucurbita moschata])

HSP 1 Score: 551 bits (1419), Expect = 1.65e-196
Identity = 274/279 (98.21%), Postives = 275/279 (98.57%), Query Frame = 0

Query: 1   MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 60
           MGQEVLNPF RRRAQFRLCNVSGYTNEL EIHADDPSLHVLFIPGNPGIVSFYKDFVESL
Sbjct: 1   MGQEVLNPFIRRRAQFRLCNVSGYTNELPEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 60

Query: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 120
           YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI
Sbjct: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 120

Query: 121 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 180
           GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTL SSFVALLGV
Sbjct: 121 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLVSSFVALLGV 180

Query: 181 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK 240
           LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMAL+EFEKLSETPDWAFMK
Sbjct: 181 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALSEFEKLSETPDWAFMK 240

Query: 241 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE 279
           KSSRKLSFLFCMDDHWAPMHVFEEI KQIPEMDVSVERE
Sbjct: 241 KSSRKLSFLFCMDDHWAPMHVFEEIWKQIPEMDVSVERE 279

BLAST of Csor.00g032890 vs. NCBI nr
Match: XP_022972587.1 (lipid droplet-associated hydrolase isoform X2 [Cucurbita maxima])

HSP 1 Score: 550 bits (1416), Expect = 4.72e-196
Identity = 273/279 (97.85%), Postives = 275/279 (98.57%), Query Frame = 0

Query: 1   MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 60
           MGQEVLNPFSR RAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYK FVESL
Sbjct: 1   MGQEVLNPFSRWRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKYFVESL 60

Query: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 120
           YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQ+KDIPLLLVGHSI
Sbjct: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQSKDIPLLLVGHSI 120

Query: 121 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 180
           GSYISIELYRKFQD+VVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV
Sbjct: 121 GSYISIELYRKFQDRVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 180

Query: 181 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK 240
           LPSRVSSFVV KTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDW FMK
Sbjct: 181 LPSRVSSFVVGKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWGFMK 240

Query: 241 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE 279
           KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE
Sbjct: 241 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE 279

BLAST of Csor.00g032890 vs. ExPASy TrEMBL
Match: A0A6J1HF89 (lipid droplet-associated hydrolase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111463638 PE=3 SV=1)

HSP 1 Score: 551 bits (1419), Expect = 7.98e-197
Identity = 274/279 (98.21%), Postives = 275/279 (98.57%), Query Frame = 0

Query: 1   MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 60
           MGQEVLNPF RRRAQFRLCNVSGYTNEL EIHADDPSLHVLFIPGNPGIVSFYKDFVESL
Sbjct: 1   MGQEVLNPFIRRRAQFRLCNVSGYTNELPEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 60

Query: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 120
           YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI
Sbjct: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 120

Query: 121 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 180
           GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTL SSFVALLGV
Sbjct: 121 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLVSSFVALLGV 180

Query: 181 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK 240
           LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMAL+EFEKLSETPDWAFMK
Sbjct: 181 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALSEFEKLSETPDWAFMK 240

Query: 241 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE 279
           KSSRKLSFLFCMDDHWAPMHVFEEI KQIPEMDVSVERE
Sbjct: 241 KSSRKLSFLFCMDDHWAPMHVFEEIWKQIPEMDVSVERE 279

BLAST of Csor.00g032890 vs. ExPASy TrEMBL
Match: A0A6J1I581 (lipid droplet-associated hydrolase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111471128 PE=3 SV=1)

HSP 1 Score: 550 bits (1416), Expect = 2.29e-196
Identity = 273/279 (97.85%), Postives = 275/279 (98.57%), Query Frame = 0

Query: 1   MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 60
           MGQEVLNPFSR RAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYK FVESL
Sbjct: 1   MGQEVLNPFSRWRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKYFVESL 60

Query: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 120
           YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQ+KDIPLLLVGHSI
Sbjct: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQSKDIPLLLVGHSI 120

Query: 121 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 180
           GSYISIELYRKFQD+VVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV
Sbjct: 121 GSYISIELYRKFQDRVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 180

Query: 181 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK 240
           LPSRVSSFVV KTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDW FMK
Sbjct: 181 LPSRVSSFVVGKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWGFMK 240

Query: 241 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE 279
           KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE
Sbjct: 241 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE 279

BLAST of Csor.00g032890 vs. ExPASy TrEMBL
Match: A0A6J1HG44 (lipid droplet-associated hydrolase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111463638 PE=3 SV=1)

HSP 1 Score: 551 bits (1419), Expect = 3.59e-196
Identity = 274/279 (98.21%), Postives = 275/279 (98.57%), Query Frame = 0

Query: 1   MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 60
           MGQEVLNPF RRRAQFRLCNVSGYTNEL EIHADDPSLHVLFIPGNPGIVSFYKDFVESL
Sbjct: 42  MGQEVLNPFIRRRAQFRLCNVSGYTNELPEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 101

Query: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 120
           YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI
Sbjct: 102 YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 161

Query: 121 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 180
           GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTL SSFVALLGV
Sbjct: 162 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLVSSFVALLGV 221

Query: 181 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK 240
           LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMAL+EFEKLSETPDWAFMK
Sbjct: 222 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALSEFEKLSETPDWAFMK 281

Query: 241 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE 279
           KSSRKLSFLFCMDDHWAPMHVFEEI KQIPEMDVSVERE
Sbjct: 282 KSSRKLSFLFCMDDHWAPMHVFEEIWKQIPEMDVSVERE 320

BLAST of Csor.00g032890 vs. ExPASy TrEMBL
Match: A0A6J1I921 (lipid droplet-associated hydrolase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471128 PE=3 SV=1)

HSP 1 Score: 550 bits (1416), Expect = 1.07e-195
Identity = 273/279 (97.85%), Postives = 275/279 (98.57%), Query Frame = 0

Query: 1   MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 60
           MGQEVLNPFSR RAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYK FVESL
Sbjct: 43  MGQEVLNPFSRWRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKYFVESL 102

Query: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 120
           YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQ+KDIPLLLVGHSI
Sbjct: 103 YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQSKDIPLLLVGHSI 162

Query: 121 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 180
           GSYISIELYRKFQD+VVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV
Sbjct: 163 GSYISIELYRKFQDRVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 222

Query: 181 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK 240
           LPSRVSSFVV KTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDW FMK
Sbjct: 223 LPSRVSSFVVGKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWGFMK 282

Query: 241 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE 279
           KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE
Sbjct: 283 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVERE 321

BLAST of Csor.00g032890 vs. ExPASy TrEMBL
Match: A0A6J1IV27 (lipid droplet-associated hydrolase-like OS=Cucurbita maxima OX=3661 GN=LOC111478816 PE=3 SV=1)

HSP 1 Score: 490 bits (1261), Expect = 9.65e-173
Identity = 241/299 (80.60%), Postives = 263/299 (87.96%), Query Frame = 0

Query: 1   MGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESL 60
           M QE L  FSRRR +FRLCNVSGYTNELLEIH+D+PSLHVLFIPGNPGI+SFYKDFVESL
Sbjct: 1   MDQEDLQSFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKDFVESL 60

Query: 61  YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSI 120
           YQLLGGRVSITAIGH+ QT KDWEGGRLFSLQEQIDHKIEFV+QELQNK+IPLLLVGHSI
Sbjct: 61  YQLLGGRVSITAIGHICQTNKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120

Query: 121 GSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 180
           GS ISIEL R F D+VVYCIGL+PFMMVNRQSR Q LI KLAGS LLSTLFSSF ALLGV
Sbjct: 121 GSPISIELCRWFPDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSTLFSSFAALLGV 180

Query: 181 LPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMK 240
           LP R SSFVV KTIGKSWSRTASEAAC++LLKYH MRN+L+MA TEFEKLSETPDW FMK
Sbjct: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAKTEFEKLSETPDWKFMK 240

Query: 241 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVEREESHLRLSSSACFSTMNVSPY 299
           K+S KLSFLFC DDHWAPMHVFEEI KQ+PE+DVSVEREE H      +   +++++ Y
Sbjct: 241 KASEKLSFLFCKDDHWAPMHVFEEICKQVPEVDVSVEREEGHKHAFCCSEAGSLHIAQY 299

BLAST of Csor.00g032890 vs. TAIR 10
Match: AT3G11620.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 349.0 bits (894), Expect = 4.0e-96
Identity = 161/270 (59.63%), Postives = 212/270 (78.52%), Query Frame = 0

Query: 10  SRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESLYQLLGGRVS 69
           ++R  + RLC VSG   E++EI A++P+ HVLFIPGNPG+VSFYKDF+ESLY+ LGG  S
Sbjct: 11  TKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYEFLGGNAS 70

Query: 70  ITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSIGSYISIELY 129
           + AIG +S T+KDWE GRLFS QEQIDHKI+F+RQEL++  +P++LVGHSIGSYIS+EL 
Sbjct: 71  VIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGSYISLELL 130

Query: 130 RKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGVLPSRVSSFV 189
           +KF D+VVYCIGL+PF+ +N+QS +Q LIGKLA S +LS   S  +A L +LP   +  +
Sbjct: 131 KKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLPMSAARLL 190

Query: 190 VSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMKKSSRKLSFL 249
           VSK+IG SWS TA +A CT+L +YH MRN+LFMA +EF +L+  PDW FM+++  KL+FL
Sbjct: 191 VSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFRELAAEPDWDFMRENQSKLAFL 250

Query: 250 FCMDDHWAPMHVFEEISKQIPEMDVSVERE 280
           F +DDHW P+ +FEEISKQ P   +S+ERE
Sbjct: 251 FGIDDHWGPLQLFEEISKQAPHTSLSIERE 280

BLAST of Csor.00g032890 vs. TAIR 10
Match: AT3G11620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 349.0 bits (894), Expect = 4.0e-96
Identity = 161/270 (59.63%), Postives = 212/270 (78.52%), Query Frame = 0

Query: 10  SRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESLYQLLGGRVS 69
           ++R  + RLC VSG   E++EI A++P+ HVLFIPGNPG+VSFYKDF+ESLY+ LGG  S
Sbjct: 11  TKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYEFLGGNAS 70

Query: 70  ITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSIGSYISIELY 129
           + AIG +S T+KDWE GRLFS QEQIDHKI+F+RQEL++  +P++LVGHSIGSYIS+EL 
Sbjct: 71  VIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGSYISLELL 130

Query: 130 RKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGVLPSRVSSFV 189
           +KF D+VVYCIGL+PF+ +N+QS +Q LIGKLA S +LS   S  +A L +LP   +  +
Sbjct: 131 KKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLPMSAARLL 190

Query: 190 VSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWAFMKKSSRKLSFL 249
           VSK+IG SWS TA +A CT+L +YH MRN+LFMA +EF +L+  PDW FM+++  KL+FL
Sbjct: 191 VSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFRELAAEPDWDFMRENQSKLAFL 250

Query: 250 FCMDDHWAPMHVFEEISKQIPEMDVSVERE 280
           F +DDHW P+ +FEEISKQ P   +S+ERE
Sbjct: 251 FGIDDHWGPLQLFEEISKQAPHTSLSIERE 280

BLAST of Csor.00g032890 vs. TAIR 10
Match: AT3G11620.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 285.8 bits (730), Expect = 4.2e-77
Identity = 133/221 (60.18%), Postives = 175/221 (79.19%), Query Frame = 0

Query: 10  SRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESLYQLLGGRVS 69
           ++R  + RLC VSG   E++EI A++P+ HVLFIPGNPG+VSFYKDF+ESLY+ LGG  S
Sbjct: 11  TKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYEFLGGNAS 70

Query: 70  ITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSIGSYISIELY 129
           + AIG +S T+KDWE GRLFS QEQIDHKI+F+RQEL++  +P++LVGHSIGSYIS+EL 
Sbjct: 71  VIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGSYISLELL 130

Query: 130 RKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGVLPSRVSSFV 189
           +KF D+VVYCIGL+PF+ +N+QS +Q LIGKLA S +LS   S  +A L +LP   +  +
Sbjct: 131 KKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLPMSAARLL 190

Query: 190 VSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKL 231
           VSK+IG SWS TA +A CT+L +YH MRN+LFMA +EF ++
Sbjct: 191 VSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFREV 231

BLAST of Csor.00g032890 vs. TAIR 10
Match: AT3G11620.4 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 285.8 bits (730), Expect = 4.2e-77
Identity = 133/221 (60.18%), Postives = 175/221 (79.19%), Query Frame = 0

Query: 10  SRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKDFVESLYQLLGGRVS 69
           ++R  + RLC VSG   E++EI A++P+ HVLFIPGNPG+VSFYKDF+ESLY+ LGG  S
Sbjct: 11  TKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYEFLGGNAS 70

Query: 70  ITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSIGSYISIELY 129
           + AIG +S T+KDWE GRLFS QEQIDHKI+F+RQEL++  +P++LVGHSIGSYIS+EL 
Sbjct: 71  VIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGSYISLELL 130

Query: 130 RKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGVLPSRVSSFV 189
           +KF D+VVYCIGL+PF+ +N+QS +Q LIGKLA S +LS   S  +A L +LP   +  +
Sbjct: 131 KKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLPMSAARLL 190

Query: 190 VSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKL 231
           VSK+IG SWS TA +A CT+L +YH MRN+LFMA +EF ++
Sbjct: 191 VSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFREV 231

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5F4771.9e-2129.53Lipid droplet-associated hydrolase OS=Gallus gallus OX=9031 GN=LDAH PE=2 SV=2[more]
Q54LL83.5e-2030.11Lipid droplet-associated hydrolase OS=Dictyostelium discoideum OX=44689 GN=DDB_G... [more]
Q5HZX71.1e-1828.40Lipid droplet-associated hydrolase OS=Rattus norvegicus OX=10116 GN=Ldah PE=2 SV... [more]
Q8BVA53.3e-1826.47Lipid droplet-associated hydrolase OS=Mus musculus OX=10090 GN=Ldah PE=1 SV=1[more]
Q9H6V91.4e-1626.95Lipid droplet-associated hydrolase OS=Homo sapiens OX=9606 GN=LDAH PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6595161.15.38e-228100.00Lipid droplet-associated hydrolase, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023517069.13.11e-201100.00lipid droplet-associated hydrolase isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023517065.11.41e-200100.00lipid droplet-associated hydrolase isoform X1 [Cucurbita pepo subsp. pepo] >XP_0... [more]
XP_022963432.11.65e-19698.21lipid droplet-associated hydrolase isoform X2 [Cucurbita moschata][more]
XP_022972587.14.72e-19697.85lipid droplet-associated hydrolase isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1HF897.98e-19798.21lipid droplet-associated hydrolase isoform X2 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1I5812.29e-19697.85lipid droplet-associated hydrolase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1HG443.59e-19698.21lipid droplet-associated hydrolase isoform X1 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1I9211.07e-19597.85lipid droplet-associated hydrolase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1IV279.65e-17380.60lipid droplet-associated hydrolase-like OS=Cucurbita maxima OX=3661 GN=LOC111478... [more]
Match NameE-valueIdentityDescription
AT3G11620.24.0e-9659.63alpha/beta-Hydrolases superfamily protein [more]
AT3G11620.14.0e-9659.63alpha/beta-Hydrolases superfamily protein [more]
AT3G11620.34.2e-7760.18alpha/beta-Hydrolases superfamily protein [more]
AT3G11620.44.2e-7760.18alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 33..285
e-value: 9.2E-11
score: 44.1
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 35..276
IPR019363Lipid droplet-associated hydrolasePFAMPF10230LIDHydrolasecoord: 38..279
e-value: 1.1E-60
score: 205.4
IPR019363Lipid droplet-associated hydrolasePANTHERPTHR13390LIPASEcoord: 16..281
NoneNo IPR availablePANTHERPTHR13390:SF2BNAA05G27450D PROTEINcoord: 16..281

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g032890.m01Csor.00g032890.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019915 lipid storage
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005811 lipid droplet
molecular_function GO:0016298 lipase activity