Homology
BLAST of Csor.00g031160 vs. ExPASy Swiss-Prot
Match:
Q9FJ79 (DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1)
HSP 1 Score: 1000.0 bits (2584), Expect = 2.2e-290
Identity = 559/931 (60.04%), Postives = 678/931 (72.82%), Query Frame = 0
Query: 1 MAVETFGKAPM------FDDDDEDNMPIVFKRSS-----ASRQNQLNSEVKHSQSQRLNQ 60
MA E F K + ++D+DED++P+VFKR+S +R + +N+ +++S
Sbjct: 1 MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAI----- 60
Query: 61 LSGRPGSDACSANGQS--SNAQKRMTSLPKTSPMRPEI-GSQKAAKSSIQEPLVKSLGTT 120
GS S +S ++ + +S ++S M+P + S +S+++ PL
Sbjct: 61 -----GSTKSSPPMRSPLTSPNRSASSSTRSSMMKPALPSSSSVQRSTLKSPLRDDRSVV 120
Query: 121 LKVSDSMDSRPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPT- 180
K + P +V + +D DSEDDKPLS RLK +S K P +
Sbjct: 121 AKERNGFGKAP------SVSKSDD--------EDSEDDKPLSARLKLDSKEVTKQPSSSG 180
Query: 181 -------VQKNKIKKSSEDS-------DDEVPLSSKFQMKSASGVLGGKQHGFDEKKPLD 240
VQK+ ++ D+ P+SS Q K++ G K ++K+PL
Sbjct: 181 RGSTQQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLV 240
Query: 241 SKVHQNG---STSRDKLQKPAVVSSKRPLPNADSHSSSQISLKKPKLS------------ 300
+ + +NG T Q PA KRPL SS+Q S+K+PKLS
Sbjct: 241 NNIDRNGLKPKTEGHSSQAPA----KRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGS 300
Query: 301 -------------DSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGSKKT 360
D+S PL N ++ K++ D DDH+PI+ RMK ++ NKSS +K +
Sbjct: 301 HISATQDAKGKNLDASKPLRANQATV--KEDNSDGDDHVPIASRMKSDSSNNKSSSAKPS 360
Query: 361 VSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHG 420
SK+ +SS + +K K VK SKY KS+K LP SGDGQKKW TL HNGVIFPPPY+ HG
Sbjct: 361 SSKMIASSSRTIAQKPNKWVKDSKYSKSSKSLP-SGDGQKKWKTLQHNGVIFPPPYKRHG 420
Query: 421 VKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 480
VKIL +GKPVDLTPEQEEVATMFAVM++T+Y +KPKF+ENFWNDWRKLLGKNH+I++LDD
Sbjct: 421 VKILFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDD 480
Query: 481 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPG 540
CDFTPIY+WY EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E++GNFRVEPPG
Sbjct: 481 CDFTPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPG 540
Query: 541 LFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWND 600
LFRGRGEHPKMGKLKKRIRP DITINIGK+APIPECPIPG+RWKEV+HDNTVTWLAFW+D
Sbjct: 541 LFRGRGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSD 600
Query: 601 PINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAV 660
PINPKEFKYVFLAASSSLKGQSDKEKYEKAR L ++I +IR +YTKDF +KD T+RQIAV
Sbjct: 601 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAV 660
Query: 661 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 720
ATYLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEV
Sbjct: 661 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEV 720
Query: 721 ELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 780
E V+KAI QFQ+GKS +DDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML+
Sbjct: 721 EPLVYKAIGQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLS 780
Query: 781 RGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKID 840
+ T+DG+V EK+V YQ ANKEVAIICNHQRTVSKSHGAQ+ +L KI ELR+ +KEL ID
Sbjct: 781 KETRDGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNID 840
Query: 841 LDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSK 875
LDRAKKG+ PL GSDGK+KRNLTPEALEKKI QT KIEK+ERDM+TKED+KTVALGTSK
Sbjct: 841 LDRAKKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSK 898
BLAST of Csor.00g031160 vs. ExPASy Swiss-Prot
Match:
P30181 (DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1)
HSP 1 Score: 992.3 bits (2564), Expect = 4.6e-288
Identity = 558/927 (60.19%), Postives = 671/927 (72.38%), Query Frame = 0
Query: 1 MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLNSEVKHSQSQRLNQLSGRPG 60
M ET K P+ D+ D +D+ P+ FKR++ AS NQ S + S++ ++S
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60
Query: 61 SDACSANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDS 120
S S NG T KTS ++ + S + S + PL + +T+K +
Sbjct: 61 SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120
Query: 121 RPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGN----SNHPIKGPIPTVQKN-- 180
+ D E+ DSEDD+PLS L GN S+ + P P + N
Sbjct: 121 ------------QKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180
Query: 181 -KIKKSS----EDSDDEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDK 240
+ ++S ++SDDE P+SS F+ K SG+ GG Q DEKKPL K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240
Query: 241 LQKPAVVSSKRPLPNADSHSSSQISLKKPKLSDSSTPLSTNTVS---------------- 300
+ V+ KRPL + S+ Q SLKK K+S S T + S
Sbjct: 241 VPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPK 300
Query: 301 LKAKQEVV-------DDDDHIPISQRMKMSAAVNKSSGSKKTVSKVASSS---------- 360
+KAKQ DDDD +PIS+R K ++ + +S +K K+ S+S
Sbjct: 301 MKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNV 360
Query: 361 ----IKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHGVKIL 420
+ K +KK K SKY S+K PSSGDGQKKWTTLVHNGVIFPPPY+ HG+KIL
Sbjct: 361 VSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKIL 420
Query: 421 HKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
+KGKPVDLT EQEEVATMFAVM++TDY KP+F+ENFWNDWR+LLGK HVIQ LDDCDFT
Sbjct: 421 YKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFT 480
Query: 481 PIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRG 540
PIY+W+ EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGLFRG
Sbjct: 481 PIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRG 540
Query: 541 RGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINP 600
RGEHPKMGKLKKRI P +IT+NIGK APIPECPI G+RWKEV+HDNTVTWLAFW DPINP
Sbjct: 541 RGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINP 600
Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYL 660
KEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR +YTK+FT+KD RQIAVATYL
Sbjct: 601 KEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYL 660
Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
IDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE V
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLV 720
Query: 721 FKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
+KAI QFQ+GKS +DDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TK
Sbjct: 721 YKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETK 780
Query: 781 DGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
DG+V++KIV YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL+++LKELK +LDRA
Sbjct: 781 DGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRA 840
Query: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 875
KKGKPPL+GSDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYL 900
BLAST of Csor.00g031160 vs. ExPASy Swiss-Prot
Match:
P93119 (DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1)
HSP 1 Score: 811.6 bits (2095), Expect = 1.1e-233
Identity = 476/792 (60.10%), Postives = 561/792 (70.83%), Query Frame = 0
Query: 125 VKEENDSIHPTQENSDSEDDKPLSIR-----LKGNSNHPIKG-----PIP-TVQKNKIKK 184
+KEE S + DS+DDKPLS + L GNS+H G P P T K +I K
Sbjct: 22 LKEEGQS---RSNSEDSDDDKPLSHKLSSGALNGNSHHIRMGSNLSCPSPYTSPKPRIIK 81
Query: 185 SSEDSDDEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSS 244
E DE+ LSS+ Q+ +G + DE KPL S +L+ P+ +
Sbjct: 82 GPE---DEMLLSSELQLN--AGASNAESSDSDESKPLAS-----------ELEDPSSSLN 141
Query: 245 KRPLPNADSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAA 304
KRPL +S KK KLS+ S P TN A+QE DD IS R K S
Sbjct: 142 KRPL--IQPKNSDPSPSKKAKLSEPSAP--TNRKLKTAEQEEAAADDDPSISNRNKKSTT 201
Query: 305 VNKSSGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGV 364
SKV+ + + S V SK +K +L +GQ LVHNGV
Sbjct: 202 ---------PASKVSDKKKRPDVSASVNKVDFSKSLKVPLVLVKGRNGQ----PLVHNGV 261
Query: 365 IFPPPYQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLG 424
IFPP Y+ HGVK+L++ PVDLTPEQEEVATMFAVM +T+YM KPKF+ENF +DWRK+LG
Sbjct: 262 IFPPLYKPHGVKMLYRENPVDLTPEQEEVATMFAVMLETEYMTKPKFRENFMSDWRKILG 321
Query: 425 KNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLK-QEEKYMWAILDGV-K 484
+ H+IQNL+DCDFTPIY+W+Q EKEKKKQM+T+EKKA+KE K + ++EKYMWA++DG+ +
Sbjct: 322 EKHIIQNLEDCDFTPIYEWHQREKEKKKQMSTDEKKAIKEGKDETRKEKYMWAVVDGMSR 381
Query: 485 EKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPS-DITINIGKDAPIPECPIPGQRWKEVR 544
EKVGNFRV + + K ++KK + DITINIGKDAPIPE PIPG+RWKE+R
Sbjct: 382 EKVGNFRVGTTRVVQRSWRASKDPQVKKNVYSQIDITINIGKDAPIPEPPIPGERWKEIR 441
Query: 545 HDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIR-NSYTK 604
HDNTVTWLAFWNDPINPKEFKYVFLAASSSLKG+ +EKYEK+R LKDYI+ IR +YTK
Sbjct: 442 HDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGKVTREKYEKSRKLKDYIEGIRAAAYTK 501
Query: 605 DFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNF 664
DF SKD +RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+ PN+L+F+F
Sbjct: 502 DFASKDSKKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVETKRPNILKFDF 561
Query: 665 LGKDSIKYENTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVF 724
LGKDSI+Y+N VEVE VF AI QF+SGK G DDLFD+LDTSKLNAHLK+LMPGLTAKVF
Sbjct: 562 LGKDSIRYQNEVEVEPRVFSAIEQFRSGKEGGDDLFDQLDTSKLNAHLKELMPGLTAKVF 621
Query: 725 RTYNASITLDDM-----LNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMT 784
RTYNASITLD+M L+R TK G+V+EK+V YQHANKEVAIICNHQRTVSKSH AQM
Sbjct: 622 RTYNASITLDEMVRCKLLSRETKGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMV 681
Query: 785 RLNEKISELRDILKELKIDLDRAKKGKPPLKGSDGKQKRNLTPE---------------- 844
RLNEKI EL+ +LKEL+ DL R KGKPPLK SDGK KRNL +
Sbjct: 682 RLNEKIEELKGLLKELQEDLTRVNKGKPPLKNSDGKPKRNLNLKRYKGKLLKLIQKLRKW 741
Query: 845 ------ALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVP 875
++ KKI+QTN KIEK+ERD +TKEDLKTVALGTSKINYLDPRITVAWCKRH+VP
Sbjct: 742 SETRRLSVTKKIAQTNTKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHDVP 777
BLAST of Csor.00g031160 vs. ExPASy Swiss-Prot
Match:
Q54RC3 (DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1)
HSP 1 Score: 516.9 bits (1330), Expect = 5.7e-145
Identity = 333/852 (39.08%), Postives = 487/852 (57.16%), Query Frame = 0
Query: 70 KRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQLANAAVKEEN 129
K++T PK +P+ P+ + K I P K + ++ SDS DS ++++ ++
Sbjct: 59 KKLTPTPKPAPVTPKPTAAVNGKKPI--PKKKPVDSS-SDSDSSDSSSDSDSDSS---DS 118
Query: 130 DSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDDEVPLSSKFQ 189
DS + ++S S+ D P + N P G + KN K ++ + + SK
Sbjct: 119 DSDSSSSDSSSSDSDVPKKKPISKIPNKP-SGTVSKTSKNSPKTTTTTTTTTTTVKSKPA 178
Query: 190 MKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNADSHSSSQIS 249
K D D + S S + +KP SK+P + +I+
Sbjct: 179 PKVKPEQSSSDDSSSDSS---DDSSDDSSSDSSSEEEKPKKKPSKKP-----AAVKKEIT 238
Query: 250 LKKPKLSDSSTPLSTNTVSLKAKQEV-VDDDDHIPISQRMKMSAAVNKSSGSKKTVSKVA 309
+KK +T T K+EV V + ++++K + + K T K
Sbjct: 239 VKK--------ETTTTTKKTPVKKEVEVKKETKTTTTKKVKKEESDDDGEDEKTTTKKKT 298
Query: 310 SSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHGVKILH 369
++ +K ++ + ++ + V D KWTTL+HNG +FPP Y HG+K ++
Sbjct: 299 TTRKRKLKEEEEGEEEEEEGVNKWWDKEDDEDDGTKWTTLIHNGPVFPPAYVPHGIKFMY 358
Query: 370 KGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLL--GKNHVIQNLDDCDF 429
GK V LTP+QEEVAT +A +TD+++K FK+NF+ +++ LL + +++ +D CDF
Sbjct: 359 DGKVVKLTPKQEEVATFYANYLETDHVKKQAFKDNFFKEFKGLLTSEQKEIVKKMDKCDF 418
Query: 430 TPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFR 489
+ I+ +T+KE++K + EEK A EK K +EK+ +A +DG KEKVGN+ +EPPGLF
Sbjct: 419 SHIHTHLKTKKEQRKARSKEEKDAETAEKKKIQEKHGFATIDGFKEKVGNYMIEPPGLFL 478
Query: 490 GRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPIN 549
GRG+HPK G LK+RI PSD+T+NIG+ IP PIPG++WKE+ H+NTVTWLAFW + +N
Sbjct: 479 GRGQHPKTGMLKQRIMPSDVTLNIGEGEVIPPSPIPGEKWKEIVHNNTVTWLAFWRESVN 538
Query: 550 PKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATY 609
FKYV+LA SS +KGQ+D++K+E AR LK+ I +IR Y K F + +RQ+AVA Y
Sbjct: 539 -GGFKYVWLAPSSKIKGQADRKKFEVARGLKNCIGDIRKGYQKSFNDESTEKRQLAVALY 598
Query: 610 LIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELP 669
LID LALR GNEK +D+ADTVGCC+L+VE++K N + +FLGKDS++Y NTV++
Sbjct: 599 LIDFLALRVGNEKGEDQADTVGCCSLRVEHIKLESNNTITLDFLGKDSMRYLNTVQIRED 658
Query: 670 VFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNR-G 729
+K + F GK +D LFD L ++LN HLK M GL+AKVFRTYNASITL L++
Sbjct: 659 AYKVLKTFVKGKKPTDLLFDSLTVTQLNNHLKKQMTGLSAKVFRTYNASITLQKELDKMD 718
Query: 730 TKDGE-VSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDL 789
TK+ + + EKI+ Y N+EVAI+CNHQ+ K+ M++++EKI ELRD+ DL
Sbjct: 719 TKEFQSIDEKILFYNRCNREVAILCNHQKAPPKNLAETMSKIDEKIQELRDLQ-----DL 778
Query: 790 DRAKKGKPPLKGSDG-----------------KQKRNLTPEALEKKIS------------ 849
R K GK P + K++ E +K+IS
Sbjct: 779 CRIKIGKTPKSQQEDIESREAERLPKLIQLAITNKKDKDKEKTDKEISFDVKAAFEKSRT 838
Query: 850 ------QTNAKIEKIERDMKT-------KEDLKTVALGTSKINYLDPRITVAWCKRHEVP 875
+ KI K E +K K++LKTVALGTSKINYLDPRITVAWCK+++VP
Sbjct: 839 LPDAKGKIKEKIAKYEEQIKKQEILKTGKDELKTVALGTSKINYLDPRITVAWCKKNKVP 881
BLAST of Csor.00g031160 vs. ExPASy Swiss-Prot
Match:
P07799 (DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=top1 PE=1 SV=2)
HSP 1 Score: 505.8 bits (1301), Expect = 1.3e-141
Identity = 342/859 (39.81%), Postives = 479/859 (55.76%), Query Frame = 0
Query: 112 SMDSRPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKI 171
S DS L+ + S + + SD E D + NHP+ + NK
Sbjct: 3 SSDSDSVSLSIRRRQRRGSSKRISMKESDEESD--------SSENHPLSESL-----NK- 62
Query: 172 KKSSEDSDDEVPL-----SSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQ 231
K SE +D++P+ SSK M ++S KK +KV NG K
Sbjct: 63 KSKSESDEDDIPIRKRRASSKKNMSNSS-----------SKK--RAKVMGNGGLKNGK-- 122
Query: 232 KPAVVSSKRPLPNADSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQ 291
K AVV + D + ++ S K ++S ++ S N K+E D DD +P+
Sbjct: 123 KTAVVKEEE-----DFNEIAKPSPKHKRVSKANG--SKNGAKSAVKKEESDTDDSVPLRA 182
Query: 292 RMKMSAAVNKSSGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWT 351
+S KS + AS++ ++ + + K+ S + D +KWT
Sbjct: 183 VSTVSLTPYKSE-----LPSGASTTQNRSPNDEEDEDEDYKWWTSENI-----DDTQKWT 242
Query: 352 TLVHNGVIFPPPYQ--THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENF 411
TL HNGVIF PPY+ VK+++ G PV+L PE EEVA +A M +TD+ + P F++NF
Sbjct: 243 TLEHNGVIFAPPYEPLPKNVKLIYDGNPVNLPPEAEEVAGFYAAMLETDHAKNPVFQDNF 302
Query: 412 WNDWRKLLGK---NHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEK 471
+ D+ K+ + NH I+ CDFT ++ ++ ++E+KK M E+KKA+K++K ++EEK
Sbjct: 303 FRDFLKVCDECNFNHNIKEFSKCDFTQMFHHFEQKREEKKSMPKEQKKAIKQKKDEEEEK 362
Query: 472 YMWAILDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPI 531
Y W ILDG KEKVGNFR+EPPGLFRGRG HPK G LK+R+ P ITINIG+ P+PE P+
Sbjct: 363 YKWCILDGRKEKVGNFRIEPPGLFRGRGSHPKTGSLKRRVYPEQITINIGEGVPVPE-PL 422
Query: 532 PGQRWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIK 591
PG +W EV+HDNTVTWLA W++ IN KYVFLAA SSLKGQSD +KYEK+R LKDYI
Sbjct: 423 PGHQWAEVKHDNTVTWLATWHENIN-NNVKYVFLAAGSSLKGQSDLKKYEKSRKLKDYID 482
Query: 592 NIRNSYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVP 651
+IR Y KD S+ RQ A YLID ALRAGNEK +DEADTVGCC+L+ E+V P
Sbjct: 483 DIRKGYRKDLKSELTVERQRGTAMYLIDVFALRAGNEKGEDEADTVGCCSLRYEHVTLKP 542
Query: 652 PNLLEFNFLGKDSIKYENTVEVELPVFKAITQF-QSGKSGSDDLFDKLDTSKLNAHLKDL 711
P + F+FLGKDSI+Y N VEV+ VFK + F + K D +FD+L T+ LN +L L
Sbjct: 543 PRTVVFDFLGKDSIRYYNEVEVDPQVFKNLKIFKRPPKKEGDLIFDRLSTNSLNKYLTSL 602
Query: 712 MPGLTAKVFRTYNASITLDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHG 771
M GL+AKVFRTYNAS T+ + L + K+ +++KI+ Y AN+ VAI+CNHQR+V+K+H
Sbjct: 603 MDGLSAKVFRTYNASYTMAEELKKMPKNLTLADKILFYNRANRTVAILCNHQRSVTKNHD 662
Query: 772 AQMTRLNEKISEL---RDILKELKIDLD-RAKKGKPP----------------------- 831
QM R E+I L R L+++ ++L+ + K KP
Sbjct: 663 VQMERFAERIKALQYQRMRLRKMMLNLEPKLAKSKPELLAKEEGITDSWIVKHHETLYEL 722
Query: 832 ---------------------------------LKGSDGKQK--------RNLTP----- 887
LK +D +K + + P
Sbjct: 723 EKEKIKKKFDRENEKLAAEDPKSVLPESELEVRLKAADELKKALDAELKSKKVDPGRSSM 782
BLAST of Csor.00g031160 vs. NCBI nr
Match:
KAG6577484.1 (DNA topoisomerase 1 alpha, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 2196 bits (5690), Expect = 0.0
Identity = 1140/1140 (100.00%), Postives = 1140/1140 (100.00%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
Query: 61 ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61 ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
Query: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180
ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180
Query: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
Query: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKKRRSFNGVSSIPFPGPSTKNIQRRPR 900
TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKKRRSFNGVSSIPFPGPSTKNIQRRPR
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKKRRSFNGVSSIPFPGPSTKNIQRRPR 900
Query: 901 EFDSLSRRGQYDVDLIHLKLCFISSSHRLVMASSDLWLKEYNEASKLGDDINGMISERSS 960
EFDSLSRRGQYDVDLIHLKLCFISSSHRLVMASSDLWLKEYNEASKLGDDINGMISERSS
Sbjct: 901 EFDSLSRRGQYDVDLIHLKLCFISSSHRLVMASSDLWLKEYNEASKLGDDINGMISERSS 960
Query: 961 VPATGPEAQRHASAIRRKITILGTKVDGLESLLLKLPVKQPLSEKEINRRKDMLAQMRSK 1020
VPATGPEAQRHASAIRRKITILGTKVDGLESLLLKLPVKQPLSEKEINRRKDMLAQMRSK
Sbjct: 961 VPATGPEAQRHASAIRRKITILGTKVDGLESLLLKLPVKQPLSEKEINRRKDMLAQMRSK 1020
Query: 1021 VNQMASTLNMSNFANRNSLLGPDMKSADVMSKTAELDNQGLVGFQRQIMKEQDEGLEKLE 1080
VNQMASTLNMSNFANRNSLLGPDMKSADVMSKTAELDNQGLVGFQRQIMKEQDEGLEKLE
Sbjct: 1021 VNQMASTLNMSNFANRNSLLGPDMKSADVMSKTAELDNQGLVGFQRQIMKEQDEGLEKLE 1080
Query: 1081 ETIRSTKHIALAVNEELDLHTRLIVRVLQDLMDFLVYLHNSIMVDLQGALGTLASYLHHS 1140
ETIRSTKHIALAVNEELDLHTRLIVRVLQDLMDFLVYLHNSIMVDLQGALGTLASYLHHS
Sbjct: 1081 ETIRSTKHIALAVNEELDLHTRLIVRVLQDLMDFLVYLHNSIMVDLQGALGTLASYLHHS 1140
BLAST of Csor.00g031160 vs. NCBI nr
Match:
KAG7015549.1 (DNA topoisomerase 1 beta [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1692 bits (4381), Expect = 0.0
Identity = 872/874 (99.77%), Postives = 873/874 (99.89%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
Query: 61 ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61 ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
Query: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180
ANAA+KEENDSIHPTQENSDSEDDKPLSIRLKGNSNHP KGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 ANAALKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180
Query: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
Query: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
BLAST of Csor.00g031160 vs. NCBI nr
Match:
XP_022932254.1 (DNA topoisomerase 1 beta-like [Cucurbita moschata] >XP_022932255.1 DNA topoisomerase 1 beta-like [Cucurbita moschata] >XP_022932256.1 DNA topoisomerase 1 beta-like [Cucurbita moschata] >XP_022932257.1 DNA topoisomerase 1 beta-like [Cucurbita moschata])
HSP 1 Score: 1685 bits (4363), Expect = 0.0
Identity = 870/874 (99.54%), Postives = 870/874 (99.54%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
Query: 61 ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61 ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
Query: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180
ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHP KGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180
Query: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
EVPLSSKFQMKSASGVLGGKQHGF EKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
Query: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
DSHSSSQIS KKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
THGVKILHKGKPVDLT EQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
BLAST of Csor.00g031160 vs. NCBI nr
Match:
XP_023553297.1 (DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1684 bits (4362), Expect = 0.0
Identity = 867/874 (99.20%), Postives = 871/874 (99.66%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
Query: 61 ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
ANGQSSNAQKRMTSLPKTSPMRPEIGSQKA+KSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61 ANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
Query: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180
ANAAVKEENDSIHPTQ+NSDSEDDKPLSIRLKGNSNHP KGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 ANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180
Query: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
EVPLSSKFQMKSASGVLGG+QHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
Query: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
DSHSSSQIS KKPKLSDSSTPLSTNTVSLKAKQEV DDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMSAAVNKSSGS 300
Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+KEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKEL 780
Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
BLAST of Csor.00g031160 vs. NCBI nr
Match:
XP_023007686.1 (DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023007687.1 DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023007688.1 DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023007689.1 DNA topoisomerase 1 beta-like [Cucurbita maxima])
HSP 1 Score: 1675 bits (4339), Expect = 0.0
Identity = 864/874 (98.86%), Postives = 867/874 (99.20%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
MAVETFGKA MFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1 MAVETFGKATMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
Query: 61 ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
NGQSSNAQKRMTSLPKTSPMRPEIGSQKA+KSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61 TNGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
Query: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180
NAAVKEENDSIHP QENSDSEDDKPLSIRLKGNSNHP KGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 VNAAVKEENDSIHPAQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180
Query: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
EVPLSSKF+MKSASGVLGGKQHGFDEKKPLDSKVHQNGSTS DKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKPAVVSSKRPLPNA 240
Query: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
DSHSSSQIS KKPKLSDSSTPLST+TVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
BLAST of Csor.00g031160 vs. ExPASy TrEMBL
Match:
A0A6J1EWI0 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1)
HSP 1 Score: 1685 bits (4363), Expect = 0.0
Identity = 870/874 (99.54%), Postives = 870/874 (99.54%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
Query: 61 ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61 ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
Query: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180
ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHP KGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180
Query: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
EVPLSSKFQMKSASGVLGGKQHGF EKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
Query: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
DSHSSSQIS KKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
THGVKILHKGKPVDLT EQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
BLAST of Csor.00g031160 vs. ExPASy TrEMBL
Match:
A0A6J1KZE0 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1)
HSP 1 Score: 1675 bits (4339), Expect = 0.0
Identity = 864/874 (98.86%), Postives = 867/874 (99.20%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
MAVETFGKA MFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1 MAVETFGKATMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
Query: 61 ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
NGQSSNAQKRMTSLPKTSPMRPEIGSQKA+KSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61 TNGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
Query: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180
NAAVKEENDSIHP QENSDSEDDKPLSIRLKGNSNHP KGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 VNAAVKEENDSIHPAQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180
Query: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
EVPLSSKF+MKSASGVLGGKQHGFDEKKPLDSKVHQNGSTS DKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKPAVVSSKRPLPNA 240
Query: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
DSHSSSQIS KKPKLSDSSTPLST+TVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
BLAST of Csor.00g031160 vs. ExPASy TrEMBL
Match:
A0A1S3BM72 (DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1)
HSP 1 Score: 1550 bits (4013), Expect = 0.0
Identity = 804/876 (91.78%), Postives = 831/876 (94.86%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQL+SE+KHSQ+QRLN+L GR GSD CS
Sbjct: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60
Query: 61 ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSS-IQEPLVKSLGTTLKVSDSMDSRPKQ 120
NGQSSN QKRMTSLPKTSPMR EIGSQKA KSS IQEPL KSLG +LK SDSMDSRPK
Sbjct: 61 TNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPKN 120
Query: 121 LANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSD 180
L NAAVKEEN SI+P+QENSDSEDDKPLSIRLKGNSN P KG + T+ KNK+KKSS DSD
Sbjct: 121 LVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDSD 180
Query: 181 DEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240
DEVPLSSKFQMK ASG +G KQ GFDEKKPL KVHQNGSTSRDKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240
Query: 241 ADSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMS-AAVNKSS 300
DSHS SQ S KKPKLSDSS+PLS+ VSLKAKQEV DDDD IPISQR+K + A VNKSS
Sbjct: 241 EDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKSS 300
Query: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
SK TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
Query: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
YQTHGVK+L+KGKPVDLTP+QEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
Query: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540
VEPPGLFRGRGEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600
AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTR 600
Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660
Query: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
NTVEVELPVFKAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720
Query: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 876
BLAST of Csor.00g031160 vs. ExPASy TrEMBL
Match:
A0A0A0L2W2 (DNA topoisomerase I OS=Cucumis sativus OX=3659 GN=Csa_3G063680 PE=3 SV=1)
HSP 1 Score: 1540 bits (3987), Expect = 0.0
Identity = 798/876 (91.10%), Postives = 828/876 (94.52%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
MAVETFGKAPMFDDDDEDNMPIVFKR+SASRQNQL+SE+KHSQ+QRL++L GR GSD CS
Sbjct: 1 MAVETFGKAPMFDDDDEDNMPIVFKRTSASRQNQLDSEIKHSQAQRLSKLPGRQGSDLCS 60
Query: 61 ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSS-IQEPLVKSLGTTLKVSDSMDSRPKQ 120
NGQSSN QKRMTSLPKTSPMR EIGSQKA++SS I EPL KSLGT+LK S SMDSRPK
Sbjct: 61 TNGQSSNNQKRMTSLPKTSPMRSEIGSQKASRSSPIPEPLTKSLGTSLKASHSMDSRPKS 120
Query: 121 LANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSD 180
L NA VKEEN SI+P+QENSDSEDDKPLSIRLKGN N P KG + TV KNK+KKS DSD
Sbjct: 121 LVNAVVKEENVSINPSQENSDSEDDKPLSIRLKGNPNQPNKGLVSTVPKNKVKKSLADSD 180
Query: 181 DEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240
DEVPLSSKFQMK ASGVLG KQHGFDEKKPL KVHQNGSTSRDKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKHASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240
Query: 241 ADSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMK-MSAAVNKSS 300
DSHS SQ KKPKL+DSS+PL++ VSLKAKQEV DDDD IPISQRMK +SA VNKSS
Sbjct: 241 EDSHSPSQFPTKKPKLTDSSSPLNSKLVSLKAKQEVDDDDDDIPISQRMKKVSATVNKSS 300
Query: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
SK T+SKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKPTISKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
Query: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
YQTHGVK+L+KGKPVDLTP QEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKMLYKGKPVDLTPPQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKA+KEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKAVKEEKLKQEEKYMWAILDGVKEKVGNFR 480
Query: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540
VEPPGLFRGRGEHPKMGKLK+RI PSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRICPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600
AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTR 600
Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660
Query: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
Query: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
DDMLNRGTK+GEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK
Sbjct: 721 DDMLNRGTKNGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 876
BLAST of Csor.00g031160 vs. ExPASy TrEMBL
Match:
A0A5A7VHI9 (DNA topoisomerase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G002170 PE=3 SV=1)
HSP 1 Score: 1539 bits (3984), Expect = 0.0
Identity = 804/894 (89.93%), Postives = 831/894 (92.95%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQL+SE+KHSQ+QRLN+L GR GSD CS
Sbjct: 1 MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60
Query: 61 ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSS-IQEPLVKSLGTTLKVSDSMDSRPKQ 120
NGQSSN QKRMTSLPKTSPMR EIGSQKA KSS IQEPL KSLG +LK SDSMDSRPK
Sbjct: 61 TNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPKN 120
Query: 121 LANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSD 180
L NAAVKEEN SI+P+QENSDSEDDKPLSIRLKGNSN P KG + T+ KNK+KKSS DSD
Sbjct: 121 LVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDSD 180
Query: 181 DEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240
DEVPLSSKFQMK ASG +G KQ GFDEKKPL KVHQNGSTSRDKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240
Query: 241 ADSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMS-AAVNKSS 300
DSHS SQ S KKPKLSDSS+PLS+ VSLKAKQEV DDDD IPISQR+K + A VNKSS
Sbjct: 241 EDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKSS 300
Query: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
SK TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
Query: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
YQTHGVK+L+KGKPVDLTP+QEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
Query: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540
VEPPGLFRGRGEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600
AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTR 600
Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660
Query: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
NTVEVELPVFKAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720
Query: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEK------------------IFNKSLLAK 874
LGTSKINYLDPRITVAWCKRHEVPIEK IFNKSLLAK
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKFKNFLLIFVIHNHNLLPQIFNKSLLAK 894
BLAST of Csor.00g031160 vs. TAIR 10
Match:
AT5G55310.1 (DNA topoisomerase 1 beta )
HSP 1 Score: 1000.0 bits (2584), Expect = 1.6e-291
Identity = 559/931 (60.04%), Postives = 678/931 (72.82%), Query Frame = 0
Query: 1 MAVETFGKAPM------FDDDDEDNMPIVFKRSS-----ASRQNQLNSEVKHSQSQRLNQ 60
MA E F K + ++D+DED++P+VFKR+S +R + +N+ +++S
Sbjct: 1 MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAI----- 60
Query: 61 LSGRPGSDACSANGQS--SNAQKRMTSLPKTSPMRPEI-GSQKAAKSSIQEPLVKSLGTT 120
GS S +S ++ + +S ++S M+P + S +S+++ PL
Sbjct: 61 -----GSTKSSPPMRSPLTSPNRSASSSTRSSMMKPALPSSSSVQRSTLKSPLRDDRSVV 120
Query: 121 LKVSDSMDSRPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPT- 180
K + P +V + +D DSEDDKPLS RLK +S K P +
Sbjct: 121 AKERNGFGKAP------SVSKSDD--------EDSEDDKPLSARLKLDSKEVTKQPSSSG 180
Query: 181 -------VQKNKIKKSSEDS-------DDEVPLSSKFQMKSASGVLGGKQHGFDEKKPLD 240
VQK+ ++ D+ P+SS Q K++ G K ++K+PL
Sbjct: 181 RGSTQQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLV 240
Query: 241 SKVHQNG---STSRDKLQKPAVVSSKRPLPNADSHSSSQISLKKPKLS------------ 300
+ + +NG T Q PA KRPL SS+Q S+K+PKLS
Sbjct: 241 NNIDRNGLKPKTEGHSSQAPA----KRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGS 300
Query: 301 -------------DSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGSKKT 360
D+S PL N ++ K++ D DDH+PI+ RMK ++ NKSS +K +
Sbjct: 301 HISATQDAKGKNLDASKPLRANQATV--KEDNSDGDDHVPIASRMKSDSSNNKSSSAKPS 360
Query: 361 VSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHG 420
SK+ +SS + +K K VK SKY KS+K LP SGDGQKKW TL HNGVIFPPPY+ HG
Sbjct: 361 SSKMIASSSRTIAQKPNKWVKDSKYSKSSKSLP-SGDGQKKWKTLQHNGVIFPPPYKRHG 420
Query: 421 VKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 480
VKIL +GKPVDLTPEQEEVATMFAVM++T+Y +KPKF+ENFWNDWRKLLGKNH+I++LDD
Sbjct: 421 VKILFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDD 480
Query: 481 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPG 540
CDFTPIY+WY EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E++GNFRVEPPG
Sbjct: 481 CDFTPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPG 540
Query: 541 LFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWND 600
LFRGRGEHPKMGKLKKRIRP DITINIGK+APIPECPIPG+RWKEV+HDNTVTWLAFW+D
Sbjct: 541 LFRGRGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSD 600
Query: 601 PINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAV 660
PINPKEFKYVFLAASSSLKGQSDKEKYEKAR L ++I +IR +YTKDF +KD T+RQIAV
Sbjct: 601 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAV 660
Query: 661 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 720
ATYLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEV
Sbjct: 661 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEV 720
Query: 721 ELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 780
E V+KAI QFQ+GKS +DDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML+
Sbjct: 721 EPLVYKAIGQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLS 780
Query: 781 RGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKID 840
+ T+DG+V EK+V YQ ANKEVAIICNHQRTVSKSHGAQ+ +L KI ELR+ +KEL ID
Sbjct: 781 KETRDGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNID 840
Query: 841 LDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSK 875
LDRAKKG+ PL GSDGK+KRNLTPEALEKKI QT KIEK+ERDM+TKED+KTVALGTSK
Sbjct: 841 LDRAKKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSK 898
BLAST of Csor.00g031160 vs. TAIR 10
Match:
AT5G55300.1 (DNA topoisomerase I alpha )
HSP 1 Score: 992.3 bits (2564), Expect = 3.3e-289
Identity = 558/927 (60.19%), Postives = 671/927 (72.38%), Query Frame = 0
Query: 1 MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLNSEVKHSQSQRLNQLSGRPG 60
M ET K P+ D+ D +D+ P+ FKR++ AS NQ S + S++ ++S
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60
Query: 61 SDACSANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDS 120
S S NG T KTS ++ + S + S + PL + +T+K +
Sbjct: 61 SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120
Query: 121 RPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGN----SNHPIKGPIPTVQKN-- 180
+ D E+ DSEDD+PLS L GN S+ + P P + N
Sbjct: 121 ------------QKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180
Query: 181 -KIKKSS----EDSDDEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDK 240
+ ++S ++SDDE P+SS F+ K SG+ GG Q DEKKPL K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240
Query: 241 LQKPAVVSSKRPLPNADSHSSSQISLKKPKLSDSSTPLSTNTVS---------------- 300
+ V+ KRPL + S+ Q SLKK K+S S T + S
Sbjct: 241 VPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPK 300
Query: 301 LKAKQEVV-------DDDDHIPISQRMKMSAAVNKSSGSKKTVSKVASSS---------- 360
+KAKQ DDDD +PIS+R K ++ + +S +K K+ S+S
Sbjct: 301 MKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNV 360
Query: 361 ----IKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHGVKIL 420
+ K +KK K SKY S+K PSSGDGQKKWTTLVHNGVIFPPPY+ HG+KIL
Sbjct: 361 VSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKIL 420
Query: 421 HKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
+KGKPVDLT EQEEVATMFAVM++TDY KP+F+ENFWNDWR+LLGK HVIQ LDDCDFT
Sbjct: 421 YKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFT 480
Query: 481 PIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRG 540
PIY+W+ EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGLFRG
Sbjct: 481 PIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRG 540
Query: 541 RGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINP 600
RGEHPKMGKLKKRI P +IT+NIGK APIPECPI G+RWKEV+HDNTVTWLAFW DPINP
Sbjct: 541 RGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINP 600
Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYL 660
KEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR +YTK+FT+KD RQIAVATYL
Sbjct: 601 KEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYL 660
Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
IDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE V
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLV 720
Query: 721 FKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
+KAI QFQ+GKS +DDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TK
Sbjct: 721 YKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETK 780
Query: 781 DGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
DG+V++KIV YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL+++LKELK +LDRA
Sbjct: 781 DGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRA 840
Query: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 875
KKGKPPL+GSDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYL 900
BLAST of Csor.00g031160 vs. TAIR 10
Match:
AT5G55300.2 (DNA topoisomerase I alpha )
HSP 1 Score: 992.3 bits (2564), Expect = 3.3e-289
Identity = 558/927 (60.19%), Postives = 671/927 (72.38%), Query Frame = 0
Query: 1 MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLNSEVKHSQSQRLNQLSGRPG 60
M ET K P+ D+ D +D+ P+ FKR++ AS NQ S + S++ ++S
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60
Query: 61 SDACSANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDS 120
S S NG T KTS ++ + S + S + PL + +T+K +
Sbjct: 61 SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120
Query: 121 RPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGN----SNHPIKGPIPTVQKN-- 180
+ D E+ DSEDD+PLS L GN S+ + P P + N
Sbjct: 121 ------------QKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180
Query: 181 -KIKKSS----EDSDDEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDK 240
+ ++S ++SDDE P+SS F+ K SG+ GG Q DEKKPL K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240
Query: 241 LQKPAVVSSKRPLPNADSHSSSQISLKKPKLSDSSTPLSTNTVS---------------- 300
+ V+ KRPL + S+ Q SLKK K+S S T + S
Sbjct: 241 VPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPK 300
Query: 301 LKAKQEVV-------DDDDHIPISQRMKMSAAVNKSSGSKKTVSKVASSS---------- 360
+KAKQ DDDD +PIS+R K ++ + +S +K K+ S+S
Sbjct: 301 MKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNV 360
Query: 361 ----IKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHGVKIL 420
+ K +KK K SKY S+K PSSGDGQKKWTTLVHNGVIFPPPY+ HG+KIL
Sbjct: 361 VSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKIL 420
Query: 421 HKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
+KGKPVDLT EQEEVATMFAVM++TDY KP+F+ENFWNDWR+LLGK HVIQ LDDCDFT
Sbjct: 421 YKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFT 480
Query: 481 PIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRG 540
PIY+W+ EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGLFRG
Sbjct: 481 PIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRG 540
Query: 541 RGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINP 600
RGEHPKMGKLKKRI P +IT+NIGK APIPECPI G+RWKEV+HDNTVTWLAFW DPINP
Sbjct: 541 RGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINP 600
Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYL 660
KEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR +YTK+FT+KD RQIAVATYL
Sbjct: 601 KEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYL 660
Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
IDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE V
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLV 720
Query: 721 FKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
+KAI QFQ+GKS +DDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TK
Sbjct: 721 YKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETK 780
Query: 781 DGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
DG+V++KIV YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL+++LKELK +LDRA
Sbjct: 781 DGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRA 840
Query: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 875
KKGKPPL+GSDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYL 900
BLAST of Csor.00g031160 vs. TAIR 10
Match:
AT5G55300.3 (DNA topoisomerase I alpha )
HSP 1 Score: 400.2 bits (1027), Expect = 5.5e-111
Identity = 210/319 (65.83%), Postives = 252/319 (79.00%), Query Frame = 0
Query: 556 AASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYLIDKLALRA 615
A SSLK +M +D +K + + S +Q++ D
Sbjct: 234 ADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPKMKAKQLSTREDGTDD----D 293
Query: 616 GNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQ 675
++ DDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE V+KAI QFQ
Sbjct: 294 DDDDDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQ 353
Query: 676 SGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGEVSEKI 735
+GKS +DDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDG+V++KI
Sbjct: 354 AGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKI 413
Query: 736 VTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPLK 795
V YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL+++LKELK +LDRAKKGKPPL+
Sbjct: 414 VVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLE 473
Query: 796 GSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAW 855
GSDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAW
Sbjct: 474 GSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAW 533
Query: 856 CKRHEVPIEKIFNKSLLAK 875
CKRHEVPIEKIF KSLL K
Sbjct: 534 CKRHEVPIEKIFTKSLLEK 548
HSP 2 Score: 94.4 bits (233), Expect = 6.5e-19
Identity = 101/316 (31.96%), Postives = 154/316 (48.73%), Query Frame = 0
Query: 1 MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLNSEVKHSQSQRLNQLSGRPG 60
M ET K P+ D+ D +D+ P+ FKR++ AS NQ S + S++ ++S
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60
Query: 61 SDACSANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDS 120
S S NG T KTS ++ + S + S + PL + +T+K +
Sbjct: 61 SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120
Query: 121 RPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGN----SNHPIKGPIPTVQKN-- 180
+ D E+ DSEDD+PLS L GN S+ + P P + N
Sbjct: 121 ------------QKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180
Query: 181 -KIKKSS----EDSDDEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDK 240
+ ++S ++SDDE P+SS F+ K SG+ GG Q DEKKPL K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240
Query: 241 LQKPAVVSSKRPLPNADSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPI 300
+ V+ KRPL + S+ Q SLKK K+S S T + S+K + +DD + +
Sbjct: 241 VPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKE---IDDKGRVLV 286
BLAST of Csor.00g031160 vs. TAIR 10
Match:
AT1G79590.1 (syntaxin of plants 52 )
HSP 1 Score: 232.6 bits (592), Expect = 1.5e-60
Identity = 125/202 (61.88%), Postives = 162/202 (80.20%), Query Frame = 0
Query: 932 ASSDLWLKEYNEASKLGDDINGMISERSSVPATGPEAQRHASAIRRKITILGTKVDGLES 991
+SSD W++EYNEA KL +DINGM+SER++ TGP+AQR ASAIRRKITILGT++D L+S
Sbjct: 3 SSSDPWMREYNEALKLSEDINGMMSERNASGLTGPDAQRRASAIRRKITILGTRLDSLQS 62
Query: 992 LLLKLPVKQPLSEKEINRRKDMLAQMRSKVNQMASTLNMSNFANRNSLLGPDMKSADVMS 1051
LL+K+P KQ +SEKE+NRRKDM+ +RSK NQ+AS LNMSNFANR+SL G D+K D ++
Sbjct: 63 LLVKVPGKQHVSEKEMNRRKDMVGNLRSKTNQVASALNMSNFANRDSLFGTDLKPDDAIN 122
Query: 1052 KTAELDNQGLVGFQRQIMKEQDEGLEKLEETIRSTKHIALAVNEELDLHTRLIVRVLQDL 1111
+ + +DNQG+V FQRQ+M+EQDEGLEKLEET+ STKHIALAVNEEL L TRLI D
Sbjct: 123 RVSGMDNQGIVVFQRQVMREQDEGLEKLEETVMSTKHIALAVNEELTLQTRLI-----DD 182
Query: 1112 MDFLVYLHNSIMVDLQGALGTL 1134
+D+ V + +S + +Q +L +
Sbjct: 183 LDYDVDITDSRLRRVQKSLALM 199
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9FJ79 | 2.2e-290 | 60.04 | DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1 | [more] |
P30181 | 4.6e-288 | 60.19 | DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1 | [more] |
P93119 | 1.1e-233 | 60.10 | DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1 | [more] |
Q54RC3 | 5.7e-145 | 39.08 | DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1 | [more] |
P07799 | 1.3e-141 | 39.81 | DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28... | [more] |
Match Name | E-value | Identity | Description | |
KAG6577484.1 | 0.0 | 100.00 | DNA topoisomerase 1 alpha, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
KAG7015549.1 | 0.0 | 99.77 | DNA topoisomerase 1 beta [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
XP_022932254.1 | 0.0 | 99.54 | DNA topoisomerase 1 beta-like [Cucurbita moschata] >XP_022932255.1 DNA topoisome... | [more] |
XP_023553297.1 | 0.0 | 99.20 | DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo] | [more] |
XP_023007686.1 | 0.0 | 98.86 | DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023007687.1 DNA topoisomera... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1EWI0 | 0.0 | 99.54 | DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1 | [more] |
A0A6J1KZE0 | 0.0 | 98.86 | DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1 | [more] |
A0A1S3BM72 | 0.0 | 91.78 | DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1 | [more] |
A0A0A0L2W2 | 0.0 | 91.10 | DNA topoisomerase I OS=Cucumis sativus OX=3659 GN=Csa_3G063680 PE=3 SV=1 | [more] |
A0A5A7VHI9 | 0.0 | 89.93 | DNA topoisomerase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G... | [more] |