Csor.00g031160 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g031160
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionDNA topoisomerase I
LocationCsor_Chr16: 6453980 .. 6464752 (-)
RNA-Seq ExpressionCsor.00g031160
SyntenyCsor.00g031160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGTTGAGACTTTCGGAAAAGCTCCAATGTTTGATGATGATGATGAGGACAACATGCCCATAGTTTTCAAAAGGAGTAGTGCATCTAGGCAAAATCAGTTAAATTCGGAAGTTAAGCATTCGCAATCTCAGAGGTTGAATCAGTTATCAGGGAGGCCAGGTTCTGATGCGTGTTCGGCCAATGGCCAAAGCTCTAATGCTCAAAAAAGGATGACTTCGTTGCCGAAGACATCGCCCATGAGGCCTGAAATAGGAAGTCAGAAAGCAGCTAAGTCCTCCATCCAGGAACCGCTTGTTAAATCACTAGGCACAACTTTGAAAGTCTCAGATTCTATGGATAGCCGGCCCAAACAGTTGGCGAATGCTGCTGTGAAAGAGGAGAATGATTCTATACATCCTACTCAAGAAAATAGTGACTCTGAAGATGATAAACCATTGAGTATTAGATTGAAAGGGAACTCTAACCATCCCATTAAAGGGCCTATACCAACAGTACAAAAGAATAAAATAAAAAAATCCTCAGAAGATTCCGATGATGAGGTTCCTTTATCATCGAAATTTCAAATGAAGTCTGCTTCAGGGGTATTGGGTGGTAAACAACATGGTTTTGATGAAAAGAAACCATTGGATTCAAAAGTTCATCAGAATGGTTCGACCTCAAGGGATAAGCTGCAAAAGCCTGCTGTTGTTTCCAGCAAGAGACCTCTTCCCAATGCAGATTCCCATTCCTCTTCTCAGATTTCATTGAAAAAGCCAAAGCTTTCAGACTCATCTACTCCTTTGAGTACCAATACAGTATCATTGAAAGCAAAACAAGAAGTAGTTGACGACGATGACCATATTCCTATTTCACAGAGAATGAAGATGTCTGCAGCAGTGAATAAGTCATCAGGTTCTAAGAAAACAGTATCAAAAGTGGCTTCTTCTTCAATCAAGAAAACACTCAAGAAATCCAAAAAGCCAGTCAAGAAATCCAAATATGTTAAGTCCACAAAGTTGCTGCCCAGTTCTGGTGATGGTCAGAAAAAATGGACCACCTTGGTTCACAATGGTGTCATTTTTCCACCTCCATACCAGACTCATGGGGTGAAGATTCTTCATAAAGGGAAGCCTGTTGATTTGACTCCTGAACAAGAGGAGGTACCCCTCCACCTGACGTGTTACATGCATAGCATTTTTTTTTCATCTTTAATTTTTTATTTTATATATTTGCATGTTATTTATGTCATCCCAGTTCATTTTTACCACTGAGTTGTGGTGAATTCTTTGGTTGATATGAAATTTTGTCATTATCAGGTTGCAACCATGTTTGCAGTGATGAAGGATACAGATTATATGCAAAAACCAAAATTCAAAGAAAATTTCTGGAATGACTGGCGCAAATTACTTGGGAAAAATCATGTCATTCAGAATTTGGATGATTGTGATTTCACCCCAATATATGACTGGTATCAGACAGAGAAGGAGAAGAAGAAACAGATGACTACAGAAGTAAGTTTGTGATGTTTTAATCTTCCTGGTTATTTATGTCTTTCCATCTTTACGAGTTACAATGCAAACAATGCTTCTCCAGATTTCTACATCTCTCTTGGGTCTCGATTTATTTATTCATTAGCAAAACCTAAGAAGTAGGGATGTTTTTGATGAAAAAGGATGACTAGTTCATTTCCAAATGTTACAAACTTCTTTTGATCTGGTTTATTTTTAGGTTTTTCAGAAATCTATCCTTTGTTTTATTGTTCGTATATTTTTCTAGATGCTTTGCTTTTATGTCAACTTGTAGGTTTTTGTTTCATGTCTTGGTTATATAGACACATTGCCAAGGTGTACAATCGAGGTGGCTTTACTCCATTTCTCAATGTGAACTATTCATTTACTGTTATAAGGAAATATGAACTATTCATCATTCAAGACAGTATTTTTGCCAATGAATGTCTAACCATAATAGTTACTTCTAGTTGATACTCAATTTCAAATTACTTTGTTTTCTATTCTAATTAAGGGTAAATTTGATGCATATTTTAAAGGAATTTTTTAATTGATTGATCAGCAATTTTGAATGAAGGCAACTATGAGCTTCAAGTCCTTTTCAGGCATTCAATTTAATTAATTTTGGTCTTACATTTCTGGGATTTGTTTGTCTAGGAGAAGAAAGCTTTGAAGGAGGAGAAACTGAAACAAGAGGAGAAGTACATGTGGGCTATTCTTGATGGAGTTAAGGAGAAGGTTATTCTCATTTGCCTTTTTCTTCGTGCAGTTTGGATTATGCTAGTCTTGAGGATATGTTTTCTTTATACTTTAATAACCTGTCATTGATGTATTCTAAGAAGCTAAGAAGCACAGGCACTTTAGTTTGACTAGTGGATGTCTGATACATATTAAACACTTGGACACTATTGAACACTTCTTAAGTACTAAGCAAGCCCATAGGATGGAAGTGAAAATTTTTGAGAACAAAATAATACTAATGGTGATGCTTGATTAATTACATTGCAAAATGTGTTAAGACACTTGTTTACGCTTATGTTTTGTCTTTTTCATACTAAATTACTAAATTCATGCATTGGTTGCTACTTAATGTACTTTGTAAGTTCATTGTAGGAATCCATTTTATCTGCGATTTTATCCTTTGAATAATTCCAGTAGAGCATGTACACACATTTTTTTGATTCTTAATATGGATCTCAGGTTGGAAACTTTAGAGTTGAACCACCTGGATTGTTCCGAGGCCGTGGAGAACATCCAAAGGTACCATGTTTAGAATTCAGAAAGTTAAATATTTACTAGCATTTATGACACACTCAGTTAATTATTCAAGGTACTCTTTGAGGAGATTAGTATATGTTTTGGTATTGGATGTTCATAGTTTAAATTTACGTGGAGATTTGTTACTTGATTCAGCTTTTAAAGCTTGGTTGTCTTTGCAGATGGGGAAGTTGAAAAAACGCATTCGCCCCAGTGACATCACAATCAATATCGGAAAAGATGCTCCGATCCCCGAGTGTCCTATACCTGGTCAAAGGTTTGCCTCTTTACACCACTGGAAATAAGTTACATTGAAAATATTGCAGAATAAGGGGTCTATTATGTGTCTAGAAATTCATGAGACACATGCCTTAACTGTCTCAGAATTTCTTGGTTTTATCTTTTTCATATTTTCATTCAGTTTTGAGTTCTTTGACAGGTGGAAGGAAGTGAGACACGACAATACGGTTACATGGTTGGCTTTCTGGAATGATCCAATAAATCCAAAAGAATTCAAGTATGTATTTCTGGCTGCTAGTAGTTCACTGAAAGGCCAGAGTGACAAAGAGAAATACGAGAAAGCAAGGATGCTCAAGGTGTGCCATTCATTATAATTTTACAATTCTTTGTTGAAGTTGTAGTATGGAATCATCATCATGTCTGTTGAATCCTTGCTGGTTTGTGTAGGACTACATAAAAAACATAAGGAATTCATATACAAAGGATTTTACGAGCAAAGACCCAACACGAAGGCAAATTGCAGTTGCCACTTATCTCATTGATAAACTGGCTCTTAGGGCTGGGAATGAGAAGGTAATGTCATTCTAGCCAATTTATGTTTCCATGCATGCATTACTTTATATTTTCAACCATTTTGATCTGGAAGAGTTAAAAGATGTTTGATCCCTACCGCAGGATGACGATGAAGCTGATACAGTTGGCTGCTGTACACTGAAAGTAGAGAATGTCAAAGCAGTACCTCCTAATCTTCTGGAGGTAAACGTCTTTAAGCTTATTGAAATAGGAGCTTGAGAGTTGAGAGATGTCCTGTTTTTAATGCCAACAAGAGATGACCAGTTTTCTTTAAATTCAGTTCAACTTCCTCGGTAAAGATTCAATCAAGTATGAAAATACTGTCGAGGTTGAGCTCCCTGTTTTCAAAGCAATCACACAATTTCAAAGCGGTGAGCTTGTTTATTTGTTTTTGTTAATCCTTTTTTTTTTCTTTTTTTTTTCTTTACACAATGATGCATCAATGCCATGACTGAGGCTGTAGAGTATCGTGTTTCTCAATTCTTCAGGAAAAAGTGGAAGCGATGATCTTTTTGACAAGCTTGATACTAGTAAATTGAATGCTCATCTGAAGGATCTCATGCCTGGTCTCACTGCAAAAGTGTTTCGTACATATAATGCTTCTATCACACTTGATGACATGGTAAAATATTATTTGAAATCTCAGTTTTTCAATTTACTCATAACTTTTTTGCGGCGAACTGATTGTGAGTTAAAAATTTGTGGTAAACTAAGGATTAAATAGAATTATCCTTTTCAAAATAACTCTAGCAGGATGTTTGATTCTTAGCCACGGGTCGGCAGAGTGCGGTTTCACAATCCCATTCTTTCTCATCCATTATAGGAAAGTCTCATGTACTCTAATCTTATTCCCAGGCTTTCCCTCTAGCCAATCAAAAAAATCTTTCTTATTCTCCCAAACCAAATACTACCTGGTTCACTTCAACTATGCACTCAAAACACATATCCTGGTCTTATAACTCTTAATTCTCAACTCTCAGGCTTCCTATTCTGATCACTGCAATCAAATGCCCCTAAAGTTCAATGAACTATTGCAATTGTGATCATTTGCTTTTCCTTAACGTTTTTTTGGGTCACAAACTTGTAAACGTTCAGTCACTTTGTCATAGGCTAGGCTCTGATAGTGGAAAATAGTTTGTTCTATATCTTGCTCTCACAAACCATTTATTTCATATTTCTACAGTTTCTTAATTGATAGTAATGATGTTAAAGTTGAATGAGATCATCAAAATTTAGATGTTTGCTCATAGGTGGTAAAATCGTGTTATTAAATGCACTCCATTGGATGGAGAAAGGCAGTTTAATGATTAACTTGCATCCAGTTTCTAGAAATCATTATGAATGTTTCCAAGATAATCCGTTAACTTTTGTGGTTTTCCTTCTTTTTCCTTGTATTTAATTTAGATTTTTAACAAGATTATTTATGGTGTTATCCATGATAGTTGAACAGAGGAACCAAAGATGGAGAAGTTTCAGAAAAGATCGTCACATACCAACACGCAAACAAGGAGGTAAATTCAGCTCCTCCTTAGGTTTTACTAGTCTAATTTCATGCCATTTGACATTAGCCACTTTTTCTATGAACTACAGGTTGCAATTATTTGTAATCACCAGAGGACTGTTTCGAAATCTCATGGGGCGCAAATGACGAGATTGAACGAGAAGATCTCTGAGCTCAGGGTTAGAACATATTTTTGTCATCGATGGTTCACCTTAGTTAAATAATGCCCTTACCAATCATTCTGTAGTTGTAGTGCATTTAGTTTATCCATCATTTGTTGTGCAGGACATTCTGAAAGAGCTGAAAATTGATCTAGATAGAGCAAAGAAAGGGAAGCCCCCTTTAAAAGGCTCCGATGGAAAGCAAAAGAGGAACTTGACCCCTGAAGCGTAAACTTCTTCTTAACTTTACTTAAGAATCCCACATCAACATCTTCATTTAGTTTTTAACTTTTAGGAATCCGTGTTACAGGTTAGAGAAGAAGATATCCCAAACGAATGCAAAGATTGAGAAGATAGAGCGAGATATGAAGACTAAAGAGGATTTGAAAACCGTAGCGTTGGGCACGTCAAAGATCAACTATCTTGATCCTCGAATCACAGTTGCATGGTGTAAGCGTCACGAAGTCCCTATCGAAAAGGTAAGCATCATTTTCTTGCATTCATGGTGCTGTGCTATATACGAGACGTTGAATCAAACCGTTTATATCTCGTTTGCTTATGGAGTTTTAAGATTAAGGCGTTAACTACTTCCACAGATATTCAATAAGTCTCTTCTAGCAAAGTTTGCTTGGGCAATGGACGTCGACCCCGAATTCAGATTCTGATTGTTGTTTATATTGCGATGGTCTAAAACCCAAATCATCCTTCGCCCCGGCTCAACCGTGAAAGAAAGTAGGAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGATGGAGTAGGAAAAATTGAAGTGTAGTATTTTGCATTACATTCAATTTTTTGTTTTTAATGGTGTTAGTTGCAGAGGGAAGTCCCATAATGTATGCTTATTATCTAATCTCTAATTATATATATTTGGGTTAAAAAAAAAGAAAAAAGAAAAAGGGTCATTTGTGACACCTCCTGTCCCCTTCCCTTCCCTTCCCCCCCCACGAAGAAGCTGTGTTATGAAAACATTTGATTATTCAAAGCTTTCAAACAGCATTTACACTAATTGTTCCAACATTTTAACCATACCTTATATACTTTTTTTTTATCTTATTATACATTTGATGATTGTATGATGACCCCCTGCCTTTCTTTCTTTTCTTTGATTTGCTGCTCCCTTTGTTTCAGTTTCAATTTTCGGGTTTAACTGGAAATTCAATGGTCTTAAATTAAACAATTAATTTTGTTACGGTGTACTAAGCTTTCAATTTCATCATATTCCATAAATTATATTCTTAATTTTTCTAAAAAGATATTAGAGCTAACTGTTTTTTATTTATTTATAAAAATTTAGAAGTTATTTTTTGTAAAAACTTTATTTTTATTGAAATTCATGAATTTTTTTTAACATTTTAATTTTTTTTTTTTTTTTTTTTTGGTTTAAATTAGAGGAAGAATTAACAAAATTCAAAAATTACGACTCTTGAGTTCAAATTGATGGAGCATTTTTACCTGAGGTTCCTAAAAAATATATTTTTGATAACAAATAAGCATTGGAATACTTTTTTTTTTTAGATAAATTTTAAATTTCTTTATTATTAGTACTTTATTTTAGATTAAATTAGTAAGTTTAATTTTTGAATTATCAAATTTAGGTCATATTTACTTCAACTTTCAATTTTTTTTCCATTTTTTTAATAAAATAGAAATTTACATGACCCAAATTACAAAAATTTTAAATCTTGTTAACACCTAATTTAATTTTATACCCAATTTATTGGTTATTATTATTATTATTATTTTTAATATGTCATTGACTCATTAGATACAAATTAAAAAAATTAAAAGTTTATTATTATTTTTTTAATACATGTTATATTATTATTTTTAATATATTAGTTATTATTTTTTTTTAATACATGTTATATTAATAATTAAGATCTCGTAATATTCAAATATCAAAGATTTACAAGTCACAACATATCATTAACATCAGTTTCAAACATTAATCATTCATAATTTTTTTAAAAAATAGGATAGGGTTCGACCCTATCTTTGAAAGTCTGACCACGAAAATGTCCACCCACAAATCGAATTGAATTTTTAATTTTTTTAAAATTTAACTTAATCTAAGTGTTATGATTTTAGTCGGATAATTCAAATTATTTAAAATTATCAGATAATATTAAAAATATTTAGAATGTGGAAAAAAATTATCTATGGGCGTCCATGTGGAGATAATATTAAAAATATTTTGCAATTAAATATTTTTTCAAAGATAATATTAAAAACATTTTGTAATTAAATATTTTATAAAAAATGAAAAAGGAAGCGTAACTAATCCATAATGAACGGAAGAGGGAAGACTACAAAACAGAAAACGACGCAGTTTTAATGGAGTTTCTTCCATTCCCTTCCCTGGACCTTCTACGAAGAACATCCAACGTCGACCACGGGAATTTGACTCCCTCTCCCGCCGTGGTCAATACGACGTTGATCTCATTCATCTCAAATTGTGCTTTATTTCCTCCTCCCACAGGTGAATTTTCTGGTCTGATTTTTTTCTTCCTTGAGCCTTTGATTCTGCTTCGTCTTCATCCATTTTGTCCGTCAATTTCTGGAAACTGGCCGACATTGCATTGGATTATTTAAACGAGTTTCGTTTGCGTTGGTTATTCCGATTATCGCATCCTTATTTTTCCGATATATACAATTAGGTTTCGTTCAGCAGACGGTAGTTTATTTTGATTGATTCTTTTTCGGCCTTGTTTCTTTTGGTAAGATGAATGATAGAAGTAGAATTATTCATCTGGTTTCGTGATATGTTTAGTTGTGATCAATTTTCCTTGTATTTTCTTACCATTTTTAATTTCATGGTAGATGGTTATATGAATTGGAATATTTTTCTTTTTAGCAAACCGAGCTATGCTAGGTAGATATTATGAATTTGAATATTTGATTATGTCATTTGGAAACTTTTAATTCAATCGCCATAGGTTAGCTTTATGGGGTTGTGACATTAGGTGGAGGGGCATGCACGCTGGATACTCTCTGATATGAACACAAAATTAACCAAAAATCCGTTTTGGTGCTTGTTTTTGGAATTGATTTTAGGAGTATTTATGAGTCTAATGGAGATAAGAGTATAATATACCGTTATTAATGCGTCTCTTCTTGTCAGGTTGGTGATGGCTTCTTCTGATTTATGGTTAAAGGAATATAATGAAGCCTCTAAGCTTGGGGATGATATCAATGGCATGATTTCTGAAAGAAGTTCCGTTCCTGCAACTGGACCAGAAGCTCAACGTCATGCCTCTGCGATTCGGAGGAAGATCACGATTTTGGGGACTAAAGTTGATGGCTTAGAGTCCCTTTTGTTGAAGCTTCCTGTAAAGCAGCCCCTGTATGTCATGTCTTAAATCGAATTTAGCAATATCCTTACTTGCATAATGCTATGCTAATGTTAGAGCATTTTGATTTGGGTTATGTTTCTGTCATGTATATACTCACGTTGCTTTAAAATCCAAGGAACAATTGTTCATCTAACCTTGAGATGTAGATGACTTTTTGTGTTCTAGAGGGATGCATAGTTGAGAAAGATATATGTACTAGTTCTTAGTTCTTGGCATCTTTGAGGGTACAGCAAATGCCATGGTTCGATCTCCCTCCTCTTCGCTACTGCACTAAATTATGAGAGACCGATGAAGGGGAGGGGAGTGAGTACGGCGGAGTTCTTGCTGGAAATGTCCTCTGTCTTGATCCTTGCATTAACAAAGGCATAAGAACTATTTTTCAATCCTAAGGGACCAAAACTTTGAGATCGAAATTGTATCTATAACGGGCTAAGCCTACTACTAGCAGATATTGTCCTCTTTGGGCTTTCCCTTTCAGGCTTCCCCTCAAGGTTTTTAAAACGCGTCTGCTAGGGAGAGGTTTCCACACCCTTATAAAGAATAATTCGTTCTCCTCTCCAACCGATGTGGGGTCTCACACTATTTTTTCTAAGTCCAAGTTTGTTCTTTCTTGTGGTTTGCAGGTCCGAAAAGGAGATAAATCGACGAAAGGACATGCTTGCTCAGATGAGATCAAAAGTAAACCAGATGGCCTCAACGCTGAACATGTCAAACTTCGCTAACCGCAACAGTTTGCTTGGCCCCGATATGAAATCAGCGGATGTAATGAGCAAAACAGCTGAACTAGACAACCAAGGACTTGTTGGTTTTCAAAGACAAATCATGAAGGGCAAGTTTTTGTGACAACTCTACTTTCAACATATTATATACTTCCTTGTATCCCTTTTGGGTCTTCTCTGATATGATTTTGAAACAGAGCAAGATGAAGGTCTTGAAAAGTTGGAGGAGACTATAAGAAGTACAAAACATATTGCTTTGGCAGTTAATGAAGAACTCGATCTTCATACTCGCCTAATTGTACGTATGATTTCTTGTTTTCTTTATTAGATTTGTTAAGCTTCCTTATTTGTCTAGGCTATGGAGGTCCACTAGGTTCTTACGGGCTTCTGGGCAGTTTTATTCTTGTCTCTTGTTTCTAGGACTCTTAGGACTAATTTCTTGAGCTAATCTCTTCTTTGGGTGTACACAAGTTCGGCAGTTTTATTCTTGTCTCTTGTTTCTAGGACTCTTAGGACTAATTTCTTGAGCTAATCTCTTCTTTGGGTGTACACAAGTCTAGACCTCTGATCGTCGCCCATGATTTTGGAGTGCAAGGTCTACTAGAAACGTATTGACCTAGGTCAACATGTAAACCGCTCTAGCTATAACACCATAATCTTAACTTGGTTCTCTTAGCATGCTTGTTAGGAAGATATTTCTATCGATCAACTAAAGCCGGGAACACATAATCTAAACGTCATCTTGGTCATATCTCAGAGCTAAGAAGATCTATTGGATTCTTGGTGCAGCTAGGTGGCTTAGCACGAACTTCCAACTCTAGGATATTTTTTAGTCGGGGAGCACTAAGATCTAGAGTCTTTGACAGTCACTCACGATCTTAAGTTGCTCAGTTTACTGGAAACGTCTTGACCTAGGTCATCATCTAGCTAACCTAACTCTATTGCCCTAGTTTTGTGCGTAGAAACATGCCCAATAGGGAGAACACAACTAAACACCTAGAGCTAAACATATTATATCATGCTAACATACAAGCAAAAAATCAAGCTCAATGGGGGAGGTAACATCCATCAATTGCTTGGATCACACATCACAATAGGCCATCATTCTTTCTAACACACGGATAGCATGTAAAACAGCATTCAACACAAATCTTATCATAGGGGCATTTTAGTAATTTACATTTTACATAATTTACGTCACACATAAAACATAAAACACGTTTCGAAAATTTCAACTCAACATACCCATCCAAAGTCGTCTTAAAGTCCTCAACATGCATTTTTTAACCACCTAACAAAAAAGAATGGTTGCATGCTAGCACATCTGCCAAATAGGGGTACTGCAAGACTTGATGGACTTCTTAGTGTACCTGCACAACTCCATTATGGTTGATTTGCAAGGGGCGCTGGGAACCCTGGCTTCCTATTTGCATCACTCTTGA

mRNA sequence

ATGGCTGTTGAGACTTTCGGAAAAGCTCCAATGTTTGATGATGATGATGAGGACAACATGCCCATAGTTTTCAAAAGGAGTAGTGCATCTAGGCAAAATCAGTTAAATTCGGAAGTTAAGCATTCGCAATCTCAGAGGTTGAATCAGTTATCAGGGAGGCCAGGTTCTGATGCGTGTTCGGCCAATGGCCAAAGCTCTAATGCTCAAAAAAGGATGACTTCGTTGCCGAAGACATCGCCCATGAGGCCTGAAATAGGAAGTCAGAAAGCAGCTAAGTCCTCCATCCAGGAACCGCTTGTTAAATCACTAGGCACAACTTTGAAAGTCTCAGATTCTATGGATAGCCGGCCCAAACAGTTGGCGAATGCTGCTGTGAAAGAGGAGAATGATTCTATACATCCTACTCAAGAAAATAGTGACTCTGAAGATGATAAACCATTGAGTATTAGATTGAAAGGGAACTCTAACCATCCCATTAAAGGGCCTATACCAACAGTACAAAAGAATAAAATAAAAAAATCCTCAGAAGATTCCGATGATGAGGTTCCTTTATCATCGAAATTTCAAATGAAGTCTGCTTCAGGGGTATTGGGTGGTAAACAACATGGTTTTGATGAAAAGAAACCATTGGATTCAAAAGTTCATCAGAATGGTTCGACCTCAAGGGATAAGCTGCAAAAGCCTGCTGTTGTTTCCAGCAAGAGACCTCTTCCCAATGCAGATTCCCATTCCTCTTCTCAGATTTCATTGAAAAAGCCAAAGCTTTCAGACTCATCTACTCCTTTGAGTACCAATACAGTATCATTGAAAGCAAAACAAGAAGTAGTTGACGACGATGACCATATTCCTATTTCACAGAGAATGAAGATGTCTGCAGCAGTGAATAAGTCATCAGGTTCTAAGAAAACAGTATCAAAAGTGGCTTCTTCTTCAATCAAGAAAACACTCAAGAAATCCAAAAAGCCAGTCAAGAAATCCAAATATGTTAAGTCCACAAAGTTGCTGCCCAGTTCTGGTGATGGTCAGAAAAAATGGACCACCTTGGTTCACAATGGTGTCATTTTTCCACCTCCATACCAGACTCATGGGGTGAAGATTCTTCATAAAGGGAAGCCTGTTGATTTGACTCCTGAACAAGAGGAGGTTGCAACCATGTTTGCAGTGATGAAGGATACAGATTATATGCAAAAACCAAAATTCAAAGAAAATTTCTGGAATGACTGGCGCAAATTACTTGGGAAAAATCATGTCATTCAGAATTTGGATGATTGTGATTTCACCCCAATATATGACTGGTATCAGACAGAGAAGGAGAAGAAGAAACAGATGACTACAGAAGAGAAGAAAGCTTTGAAGGAGGAGAAACTGAAACAAGAGGAGAAGTACATGTGGGCTATTCTTGATGGAGTTAAGGAGAAGGTTGGAAACTTTAGAGTTGAACCACCTGGATTGTTCCGAGGCCGTGGAGAACATCCAAAGATGGGGAAGTTGAAAAAACGCATTCGCCCCAGTGACATCACAATCAATATCGGAAAAGATGCTCCGATCCCCGAGTGTCCTATACCTGGTCAAAGGTGGAAGGAAGTGAGACACGACAATACGGTTACATGGTTGGCTTTCTGGAATGATCCAATAAATCCAAAAGAATTCAAGTATGTATTTCTGGCTGCTAGTAGTTCACTGAAAGGCCAGAGTGACAAAGAGAAATACGAGAAAGCAAGGATGCTCAAGGACTACATAAAAAACATAAGGAATTCATATACAAAGGATTTTACGAGCAAAGACCCAACACGAAGGCAAATTGCAGTTGCCACTTATCTCATTGATAAACTGGCTCTTAGGGCTGGGAATGAGAAGGATGACGATGAAGCTGATACAGTTGGCTGCTGTACACTGAAAGTAGAGAATGTCAAAGCAGTACCTCCTAATCTTCTGGAGTTCAACTTCCTCGGTAAAGATTCAATCAAGTATGAAAATACTGTCGAGGTTGAGCTCCCTGTTTTCAAAGCAATCACACAATTTCAAAGCGGAAAAAGTGGAAGCGATGATCTTTTTGACAAGCTTGATACTAGTAAATTGAATGCTCATCTGAAGGATCTCATGCCTGGTCTCACTGCAAAAGTGTTTCGTACATATAATGCTTCTATCACACTTGATGACATGTTGAACAGAGGAACCAAAGATGGAGAAGTTTCAGAAAAGATCGTCACATACCAACACGCAAACAAGGAGGTTGCAATTATTTGTAATCACCAGAGGACTGTTTCGAAATCTCATGGGGCGCAAATGACGAGATTGAACGAGAAGATCTCTGAGCTCAGGGACATTCTGAAAGAGCTGAAAATTGATCTAGATAGAGCAAAGAAAGGGAAGCCCCCTTTAAAAGGCTCCGATGGAAAGCAAAAGAGGAACTTGACCCCTGAAGCGTTAGAGAAGAAGATATCCCAAACGAATGCAAAGATTGAGAAGATAGAGCGAGATATGAAGACTAAAGAGGATTTGAAAACCGTAGCGTTGGGCACGTCAAAGATCAACTATCTTGATCCTCGAATCACAGTTGCATGGTGTAAGCGTCACGAAGTCCCTATCGAAAAGATATTCAATAAGTCTCTTCTAGCAAAAAAACGACGCAGTTTTAATGGAGTTTCTTCCATTCCCTTCCCTGGACCTTCTACGAAGAACATCCAACGTCGACCACGGGAATTTGACTCCCTCTCCCGCCGTGGTCAATACGACGTTGATCTCATTCATCTCAAATTGTGCTTTATTTCCTCCTCCCACAGGTTGGTGATGGCTTCTTCTGATTTATGGTTAAAGGAATATAATGAAGCCTCTAAGCTTGGGGATGATATCAATGGCATGATTTCTGAAAGAAGTTCCGTTCCTGCAACTGGACCAGAAGCTCAACGTCATGCCTCTGCGATTCGGAGGAAGATCACGATTTTGGGGACTAAAGTTGATGGCTTAGAGTCCCTTTTGTTGAAGCTTCCTGTAAAGCAGCCCCTGTCCGAAAAGGAGATAAATCGACGAAAGGACATGCTTGCTCAGATGAGATCAAAAGTAAACCAGATGGCCTCAACGCTGAACATGTCAAACTTCGCTAACCGCAACAGTTTGCTTGGCCCCGATATGAAATCAGCGGATGTAATGAGCAAAACAGCTGAACTAGACAACCAAGGACTTGTTGGTTTTCAAAGACAAATCATGAAGGAGCAAGATGAAGGTCTTGAAAAGTTGGAGGAGACTATAAGAAGTACAAAACATATTGCTTTGGCAGTTAATGAAGAACTCGATCTTCATACTCGCCTAATTGTACGGGTACTGCAAGACTTGATGGACTTCTTAGTGTACCTGCACAACTCCATTATGGTTGATTTGCAAGGGGCGCTGGGAACCCTGGCTTCCTATTTGCATCACTCTTGA

Coding sequence (CDS)

ATGGCTGTTGAGACTTTCGGAAAAGCTCCAATGTTTGATGATGATGATGAGGACAACATGCCCATAGTTTTCAAAAGGAGTAGTGCATCTAGGCAAAATCAGTTAAATTCGGAAGTTAAGCATTCGCAATCTCAGAGGTTGAATCAGTTATCAGGGAGGCCAGGTTCTGATGCGTGTTCGGCCAATGGCCAAAGCTCTAATGCTCAAAAAAGGATGACTTCGTTGCCGAAGACATCGCCCATGAGGCCTGAAATAGGAAGTCAGAAAGCAGCTAAGTCCTCCATCCAGGAACCGCTTGTTAAATCACTAGGCACAACTTTGAAAGTCTCAGATTCTATGGATAGCCGGCCCAAACAGTTGGCGAATGCTGCTGTGAAAGAGGAGAATGATTCTATACATCCTACTCAAGAAAATAGTGACTCTGAAGATGATAAACCATTGAGTATTAGATTGAAAGGGAACTCTAACCATCCCATTAAAGGGCCTATACCAACAGTACAAAAGAATAAAATAAAAAAATCCTCAGAAGATTCCGATGATGAGGTTCCTTTATCATCGAAATTTCAAATGAAGTCTGCTTCAGGGGTATTGGGTGGTAAACAACATGGTTTTGATGAAAAGAAACCATTGGATTCAAAAGTTCATCAGAATGGTTCGACCTCAAGGGATAAGCTGCAAAAGCCTGCTGTTGTTTCCAGCAAGAGACCTCTTCCCAATGCAGATTCCCATTCCTCTTCTCAGATTTCATTGAAAAAGCCAAAGCTTTCAGACTCATCTACTCCTTTGAGTACCAATACAGTATCATTGAAAGCAAAACAAGAAGTAGTTGACGACGATGACCATATTCCTATTTCACAGAGAATGAAGATGTCTGCAGCAGTGAATAAGTCATCAGGTTCTAAGAAAACAGTATCAAAAGTGGCTTCTTCTTCAATCAAGAAAACACTCAAGAAATCCAAAAAGCCAGTCAAGAAATCCAAATATGTTAAGTCCACAAAGTTGCTGCCCAGTTCTGGTGATGGTCAGAAAAAATGGACCACCTTGGTTCACAATGGTGTCATTTTTCCACCTCCATACCAGACTCATGGGGTGAAGATTCTTCATAAAGGGAAGCCTGTTGATTTGACTCCTGAACAAGAGGAGGTTGCAACCATGTTTGCAGTGATGAAGGATACAGATTATATGCAAAAACCAAAATTCAAAGAAAATTTCTGGAATGACTGGCGCAAATTACTTGGGAAAAATCATGTCATTCAGAATTTGGATGATTGTGATTTCACCCCAATATATGACTGGTATCAGACAGAGAAGGAGAAGAAGAAACAGATGACTACAGAAGAGAAGAAAGCTTTGAAGGAGGAGAAACTGAAACAAGAGGAGAAGTACATGTGGGCTATTCTTGATGGAGTTAAGGAGAAGGTTGGAAACTTTAGAGTTGAACCACCTGGATTGTTCCGAGGCCGTGGAGAACATCCAAAGATGGGGAAGTTGAAAAAACGCATTCGCCCCAGTGACATCACAATCAATATCGGAAAAGATGCTCCGATCCCCGAGTGTCCTATACCTGGTCAAAGGTGGAAGGAAGTGAGACACGACAATACGGTTACATGGTTGGCTTTCTGGAATGATCCAATAAATCCAAAAGAATTCAAGTATGTATTTCTGGCTGCTAGTAGTTCACTGAAAGGCCAGAGTGACAAAGAGAAATACGAGAAAGCAAGGATGCTCAAGGACTACATAAAAAACATAAGGAATTCATATACAAAGGATTTTACGAGCAAAGACCCAACACGAAGGCAAATTGCAGTTGCCACTTATCTCATTGATAAACTGGCTCTTAGGGCTGGGAATGAGAAGGATGACGATGAAGCTGATACAGTTGGCTGCTGTACACTGAAAGTAGAGAATGTCAAAGCAGTACCTCCTAATCTTCTGGAGTTCAACTTCCTCGGTAAAGATTCAATCAAGTATGAAAATACTGTCGAGGTTGAGCTCCCTGTTTTCAAAGCAATCACACAATTTCAAAGCGGAAAAAGTGGAAGCGATGATCTTTTTGACAAGCTTGATACTAGTAAATTGAATGCTCATCTGAAGGATCTCATGCCTGGTCTCACTGCAAAAGTGTTTCGTACATATAATGCTTCTATCACACTTGATGACATGTTGAACAGAGGAACCAAAGATGGAGAAGTTTCAGAAAAGATCGTCACATACCAACACGCAAACAAGGAGGTTGCAATTATTTGTAATCACCAGAGGACTGTTTCGAAATCTCATGGGGCGCAAATGACGAGATTGAACGAGAAGATCTCTGAGCTCAGGGACATTCTGAAAGAGCTGAAAATTGATCTAGATAGAGCAAAGAAAGGGAAGCCCCCTTTAAAAGGCTCCGATGGAAAGCAAAAGAGGAACTTGACCCCTGAAGCGTTAGAGAAGAAGATATCCCAAACGAATGCAAAGATTGAGAAGATAGAGCGAGATATGAAGACTAAAGAGGATTTGAAAACCGTAGCGTTGGGCACGTCAAAGATCAACTATCTTGATCCTCGAATCACAGTTGCATGGTGTAAGCGTCACGAAGTCCCTATCGAAAAGATATTCAATAAGTCTCTTCTAGCAAAAAAACGACGCAGTTTTAATGGAGTTTCTTCCATTCCCTTCCCTGGACCTTCTACGAAGAACATCCAACGTCGACCACGGGAATTTGACTCCCTCTCCCGCCGTGGTCAATACGACGTTGATCTCATTCATCTCAAATTGTGCTTTATTTCCTCCTCCCACAGGTTGGTGATGGCTTCTTCTGATTTATGGTTAAAGGAATATAATGAAGCCTCTAAGCTTGGGGATGATATCAATGGCATGATTTCTGAAAGAAGTTCCGTTCCTGCAACTGGACCAGAAGCTCAACGTCATGCCTCTGCGATTCGGAGGAAGATCACGATTTTGGGGACTAAAGTTGATGGCTTAGAGTCCCTTTTGTTGAAGCTTCCTGTAAAGCAGCCCCTGTCCGAAAAGGAGATAAATCGACGAAAGGACATGCTTGCTCAGATGAGATCAAAAGTAAACCAGATGGCCTCAACGCTGAACATGTCAAACTTCGCTAACCGCAACAGTTTGCTTGGCCCCGATATGAAATCAGCGGATGTAATGAGCAAAACAGCTGAACTAGACAACCAAGGACTTGTTGGTTTTCAAAGACAAATCATGAAGGAGCAAGATGAAGGTCTTGAAAAGTTGGAGGAGACTATAAGAAGTACAAAACATATTGCTTTGGCAGTTAATGAAGAACTCGATCTTCATACTCGCCTAATTGTACGGGTACTGCAAGACTTGATGGACTTCTTAGTGTACCTGCACAACTCCATTATGGTTGATTTGCAAGGGGCGCTGGGAACCCTGGCTTCCTATTTGCATCACTCTTGA

Protein sequence

MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACSANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDDEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNADSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKKRRSFNGVSSIPFPGPSTKNIQRRPREFDSLSRRGQYDVDLIHLKLCFISSSHRLVMASSDLWLKEYNEASKLGDDINGMISERSSVPATGPEAQRHASAIRRKITILGTKVDGLESLLLKLPVKQPLSEKEINRRKDMLAQMRSKVNQMASTLNMSNFANRNSLLGPDMKSADVMSKTAELDNQGLVGFQRQIMKEQDEGLEKLEETIRSTKHIALAVNEELDLHTRLIVRVLQDLMDFLVYLHNSIMVDLQGALGTLASYLHHS
Homology
BLAST of Csor.00g031160 vs. ExPASy Swiss-Prot
Match: Q9FJ79 (DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1)

HSP 1 Score: 1000.0 bits (2584), Expect = 2.2e-290
Identity = 559/931 (60.04%), Postives = 678/931 (72.82%), Query Frame = 0

Query: 1   MAVETFGKAPM------FDDDDEDNMPIVFKRSS-----ASRQNQLNSEVKHSQSQRLNQ 60
           MA E F K  +      ++D+DED++P+VFKR+S      +R + +N+ +++S       
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAI----- 60

Query: 61  LSGRPGSDACSANGQS--SNAQKRMTSLPKTSPMRPEI-GSQKAAKSSIQEPLVKSLGTT 120
                GS   S   +S  ++  +  +S  ++S M+P +  S    +S+++ PL       
Sbjct: 61  -----GSTKSSPPMRSPLTSPNRSASSSTRSSMMKPALPSSSSVQRSTLKSPLRDDRSVV 120

Query: 121 LKVSDSMDSRPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPT- 180
            K  +     P      +V + +D         DSEDDKPLS RLK +S    K P  + 
Sbjct: 121 AKERNGFGKAP------SVSKSDD--------EDSEDDKPLSARLKLDSKEVTKQPSSSG 180

Query: 181 -------VQKNKIKKSSEDS-------DDEVPLSSKFQMKSASGVLGGKQHGFDEKKPLD 240
                  VQK+ ++             D+  P+SS  Q K++ G    K    ++K+PL 
Sbjct: 181 RGSTQQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLV 240

Query: 241 SKVHQNG---STSRDKLQKPAVVSSKRPLPNADSHSSSQISLKKPKLS------------ 300
           + + +NG    T     Q PA    KRPL      SS+Q S+K+PKLS            
Sbjct: 241 NNIDRNGLKPKTEGHSSQAPA----KRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGS 300

Query: 301 -------------DSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGSKKT 360
                        D+S PL  N  ++  K++  D DDH+PI+ RMK  ++ NKSS +K +
Sbjct: 301 HISATQDAKGKNLDASKPLRANQATV--KEDNSDGDDHVPIASRMKSDSSNNKSSSAKPS 360

Query: 361 VSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHG 420
            SK+ +SS +   +K  K VK SKY KS+K LP SGDGQKKW TL HNGVIFPPPY+ HG
Sbjct: 361 SSKMIASSSRTIAQKPNKWVKDSKYSKSSKSLP-SGDGQKKWKTLQHNGVIFPPPYKRHG 420

Query: 421 VKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 480
           VKIL +GKPVDLTPEQEEVATMFAVM++T+Y +KPKF+ENFWNDWRKLLGKNH+I++LDD
Sbjct: 421 VKILFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDD 480

Query: 481 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPG 540
           CDFTPIY+WY  EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E++GNFRVEPPG
Sbjct: 481 CDFTPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPG 540

Query: 541 LFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWND 600
           LFRGRGEHPKMGKLKKRIRP DITINIGK+APIPECPIPG+RWKEV+HDNTVTWLAFW+D
Sbjct: 541 LFRGRGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSD 600

Query: 601 PINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAV 660
           PINPKEFKYVFLAASSSLKGQSDKEKYEKAR L ++I +IR +YTKDF +KD T+RQIAV
Sbjct: 601 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAV 660

Query: 661 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 720
           ATYLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEV
Sbjct: 661 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEV 720

Query: 721 ELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 780
           E  V+KAI QFQ+GKS +DDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML+
Sbjct: 721 EPLVYKAIGQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLS 780

Query: 781 RGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKID 840
           + T+DG+V EK+V YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL ID
Sbjct: 781 KETRDGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNID 840

Query: 841 LDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSK 875
           LDRAKKG+ PL GSDGK+KRNLTPEALEKKI QT  KIEK+ERDM+TKED+KTVALGTSK
Sbjct: 841 LDRAKKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSK 898

BLAST of Csor.00g031160 vs. ExPASy Swiss-Prot
Match: P30181 (DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1)

HSP 1 Score: 992.3 bits (2564), Expect = 4.6e-288
Identity = 558/927 (60.19%), Postives = 671/927 (72.38%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLNSEVKHSQSQRLNQLSGRPG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    ++S    
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60

Query: 61  SDACSANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDS 120
           S   S NG         T   KTS ++  + S  +  S  + PL   + +T+K    +  
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120

Query: 121 RPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGN----SNHPIKGPIPTVQKN-- 180
                       + D      E+ DSEDD+PLS  L GN    S+  +  P P  + N  
Sbjct: 121 ------------QKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 -KIKKSS----EDSDDEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDK 240
             + ++S    ++SDDE P+SS F+ K  SG+ GG Q   DEKKPL  K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVVSSKRPLPNADSHSSSQISLKKPKLSDSSTPLSTNTVS---------------- 300
           +    V+  KRPL    + S+ Q SLKK K+S S T +     S                
Sbjct: 241 VPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPK 300

Query: 301 LKAKQEVV-------DDDDHIPISQRMKMSAAVNKSSGSKKTVSKVASSS---------- 360
           +KAKQ          DDDD +PIS+R K  ++ + +S +K    K+ S+S          
Sbjct: 301 MKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNV 360

Query: 361 ----IKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHGVKIL 420
                +   K +KK  K SKY  S+K  PSSGDGQKKWTTLVHNGVIFPPPY+ HG+KIL
Sbjct: 361 VSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKIL 420

Query: 421 HKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
           +KGKPVDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDCDFT
Sbjct: 421 YKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFT 480

Query: 481 PIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRG 540
           PIY+W+  EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGLFRG
Sbjct: 481 PIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRG 540

Query: 541 RGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINP 600
           RGEHPKMGKLKKRI P +IT+NIGK APIPECPI G+RWKEV+HDNTVTWLAFW DPINP
Sbjct: 541 RGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINP 600

Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYL 660
           KEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR +YTK+FT+KD   RQIAVATYL
Sbjct: 601 KEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYL 660

Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
           IDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLV 720

Query: 721 FKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
           +KAI QFQ+GKS +DDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TK
Sbjct: 721 YKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETK 780

Query: 781 DGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
           DG+V++KIV YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LDRA
Sbjct: 781 DGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRA 840

Query: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 875
           KKGKPPL+GSDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYL 900

BLAST of Csor.00g031160 vs. ExPASy Swiss-Prot
Match: P93119 (DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 1.1e-233
Identity = 476/792 (60.10%), Postives = 561/792 (70.83%), Query Frame = 0

Query: 125 VKEENDSIHPTQENSDSEDDKPLSIR-----LKGNSNHPIKG-----PIP-TVQKNKIKK 184
           +KEE  S      + DS+DDKPLS +     L GNS+H   G     P P T  K +I K
Sbjct: 22  LKEEGQS---RSNSEDSDDDKPLSHKLSSGALNGNSHHIRMGSNLSCPSPYTSPKPRIIK 81

Query: 185 SSEDSDDEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSS 244
             E   DE+ LSS+ Q+   +G    +    DE KPL S           +L+ P+   +
Sbjct: 82  GPE---DEMLLSSELQLN--AGASNAESSDSDESKPLAS-----------ELEDPSSSLN 141

Query: 245 KRPLPNADSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAA 304
           KRPL      +S     KK KLS+ S P  TN     A+QE    DD   IS R K S  
Sbjct: 142 KRPL--IQPKNSDPSPSKKAKLSEPSAP--TNRKLKTAEQEEAAADDDPSISNRNKKSTT 201

Query: 305 VNKSSGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGV 364
                      SKV+    +  +  S   V  SK +K   +L    +GQ     LVHNGV
Sbjct: 202 ---------PASKVSDKKKRPDVSASVNKVDFSKSLKVPLVLVKGRNGQ----PLVHNGV 261

Query: 365 IFPPPYQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLG 424
           IFPP Y+ HGVK+L++  PVDLTPEQEEVATMFAVM +T+YM KPKF+ENF +DWRK+LG
Sbjct: 262 IFPPLYKPHGVKMLYRENPVDLTPEQEEVATMFAVMLETEYMTKPKFRENFMSDWRKILG 321

Query: 425 KNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLK-QEEKYMWAILDGV-K 484
           + H+IQNL+DCDFTPIY+W+Q EKEKKKQM+T+EKKA+KE K + ++EKYMWA++DG+ +
Sbjct: 322 EKHIIQNLEDCDFTPIYEWHQREKEKKKQMSTDEKKAIKEGKDETRKEKYMWAVVDGMSR 381

Query: 485 EKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPS-DITINIGKDAPIPECPIPGQRWKEVR 544
           EKVGNFRV    + +      K  ++KK +    DITINIGKDAPIPE PIPG+RWKE+R
Sbjct: 382 EKVGNFRVGTTRVVQRSWRASKDPQVKKNVYSQIDITINIGKDAPIPEPPIPGERWKEIR 441

Query: 545 HDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIR-NSYTK 604
           HDNTVTWLAFWNDPINPKEFKYVFLAASSSLKG+  +EKYEK+R LKDYI+ IR  +YTK
Sbjct: 442 HDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGKVTREKYEKSRKLKDYIEGIRAAAYTK 501

Query: 605 DFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNF 664
           DF SKD  +RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+   PN+L+F+F
Sbjct: 502 DFASKDSKKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVETKRPNILKFDF 561

Query: 665 LGKDSIKYENTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVF 724
           LGKDSI+Y+N VEVE  VF AI QF+SGK G DDLFD+LDTSKLNAHLK+LMPGLTAKVF
Sbjct: 562 LGKDSIRYQNEVEVEPRVFSAIEQFRSGKEGGDDLFDQLDTSKLNAHLKELMPGLTAKVF 621

Query: 725 RTYNASITLDDM-----LNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMT 784
           RTYNASITLD+M     L+R TK G+V+EK+V YQHANKEVAIICNHQRTVSKSH AQM 
Sbjct: 622 RTYNASITLDEMVRCKLLSRETKGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMV 681

Query: 785 RLNEKISELRDILKELKIDLDRAKKGKPPLKGSDGKQKRNLTPE---------------- 844
           RLNEKI EL+ +LKEL+ DL R  KGKPPLK SDGK KRNL  +                
Sbjct: 682 RLNEKIEELKGLLKELQEDLTRVNKGKPPLKNSDGKPKRNLNLKRYKGKLLKLIQKLRKW 741

Query: 845 ------ALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVP 875
                 ++ KKI+QTN KIEK+ERD +TKEDLKTVALGTSKINYLDPRITVAWCKRH+VP
Sbjct: 742 SETRRLSVTKKIAQTNTKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHDVP 777

BLAST of Csor.00g031160 vs. ExPASy Swiss-Prot
Match: Q54RC3 (DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 5.7e-145
Identity = 333/852 (39.08%), Postives = 487/852 (57.16%), Query Frame = 0

Query: 70  KRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQLANAAVKEEN 129
           K++T  PK +P+ P+  +    K  I  P  K + ++   SDS DS     ++++   ++
Sbjct: 59  KKLTPTPKPAPVTPKPTAAVNGKKPI--PKKKPVDSS-SDSDSSDSSSDSDSDSS---DS 118

Query: 130 DSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDDEVPLSSKFQ 189
           DS   + ++S S+ D P    +    N P  G +    KN  K ++  +     + SK  
Sbjct: 119 DSDSSSSDSSSSDSDVPKKKPISKIPNKP-SGTVSKTSKNSPKTTTTTTTTTTTVKSKPA 178

Query: 190 MKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNADSHSSSQIS 249
            K             D     D     + S S  + +KP    SK+P     +    +I+
Sbjct: 179 PKVKPEQSSSDDSSSDSS---DDSSDDSSSDSSSEEEKPKKKPSKKP-----AAVKKEIT 238

Query: 250 LKKPKLSDSSTPLSTNTVSLKAKQEV-VDDDDHIPISQRMKMSAAVNKSSGSKKTVSKVA 309
           +KK          +T T     K+EV V  +     ++++K   + +     K T  K  
Sbjct: 239 VKK--------ETTTTTKKTPVKKEVEVKKETKTTTTKKVKKEESDDDGEDEKTTTKKKT 298

Query: 310 SSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHGVKILH 369
           ++  +K  ++ +   ++ + V          D   KWTTL+HNG +FPP Y  HG+K ++
Sbjct: 299 TTRKRKLKEEEEGEEEEEEGVNKWWDKEDDEDDGTKWTTLIHNGPVFPPAYVPHGIKFMY 358

Query: 370 KGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLL--GKNHVIQNLDDCDF 429
            GK V LTP+QEEVAT +A   +TD+++K  FK+NF+ +++ LL   +  +++ +D CDF
Sbjct: 359 DGKVVKLTPKQEEVATFYANYLETDHVKKQAFKDNFFKEFKGLLTSEQKEIVKKMDKCDF 418

Query: 430 TPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFR 489
           + I+   +T+KE++K  + EEK A   EK K +EK+ +A +DG KEKVGN+ +EPPGLF 
Sbjct: 419 SHIHTHLKTKKEQRKARSKEEKDAETAEKKKIQEKHGFATIDGFKEKVGNYMIEPPGLFL 478

Query: 490 GRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPIN 549
           GRG+HPK G LK+RI PSD+T+NIG+   IP  PIPG++WKE+ H+NTVTWLAFW + +N
Sbjct: 479 GRGQHPKTGMLKQRIMPSDVTLNIGEGEVIPPSPIPGEKWKEIVHNNTVTWLAFWRESVN 538

Query: 550 PKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATY 609
              FKYV+LA SS +KGQ+D++K+E AR LK+ I +IR  Y K F  +   +RQ+AVA Y
Sbjct: 539 -GGFKYVWLAPSSKIKGQADRKKFEVARGLKNCIGDIRKGYQKSFNDESTEKRQLAVALY 598

Query: 610 LIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELP 669
           LID LALR GNEK +D+ADTVGCC+L+VE++K    N +  +FLGKDS++Y NTV++   
Sbjct: 599 LIDFLALRVGNEKGEDQADTVGCCSLRVEHIKLESNNTITLDFLGKDSMRYLNTVQIRED 658

Query: 670 VFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNR-G 729
            +K +  F  GK  +D LFD L  ++LN HLK  M GL+AKVFRTYNASITL   L++  
Sbjct: 659 AYKVLKTFVKGKKPTDLLFDSLTVTQLNNHLKKQMTGLSAKVFRTYNASITLQKELDKMD 718

Query: 730 TKDGE-VSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDL 789
           TK+ + + EKI+ Y   N+EVAI+CNHQ+   K+    M++++EKI ELRD+      DL
Sbjct: 719 TKEFQSIDEKILFYNRCNREVAILCNHQKAPPKNLAETMSKIDEKIQELRDLQ-----DL 778

Query: 790 DRAKKGKPPLKGSDG-----------------KQKRNLTPEALEKKIS------------ 849
            R K GK P    +                    K++   E  +K+IS            
Sbjct: 779 CRIKIGKTPKSQQEDIESREAERLPKLIQLAITNKKDKDKEKTDKEISFDVKAAFEKSRT 838

Query: 850 ------QTNAKIEKIERDMKT-------KEDLKTVALGTSKINYLDPRITVAWCKRHEVP 875
                 +   KI K E  +K        K++LKTVALGTSKINYLDPRITVAWCK+++VP
Sbjct: 839 LPDAKGKIKEKIAKYEEQIKKQEILKTGKDELKTVALGTSKINYLDPRITVAWCKKNKVP 881

BLAST of Csor.00g031160 vs. ExPASy Swiss-Prot
Match: P07799 (DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=top1 PE=1 SV=2)

HSP 1 Score: 505.8 bits (1301), Expect = 1.3e-141
Identity = 342/859 (39.81%), Postives = 479/859 (55.76%), Query Frame = 0

Query: 112 SMDSRPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKI 171
           S DS    L+    +    S   + + SD E D         + NHP+   +     NK 
Sbjct: 3   SSDSDSVSLSIRRRQRRGSSKRISMKESDEESD--------SSENHPLSESL-----NK- 62

Query: 172 KKSSEDSDDEVPL-----SSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQ 231
           K  SE  +D++P+     SSK  M ++S            KK   +KV  NG     K  
Sbjct: 63  KSKSESDEDDIPIRKRRASSKKNMSNSS-----------SKK--RAKVMGNGGLKNGK-- 122

Query: 232 KPAVVSSKRPLPNADSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQ 291
           K AVV  +      D +  ++ S K  ++S ++   S N      K+E  D DD +P+  
Sbjct: 123 KTAVVKEEE-----DFNEIAKPSPKHKRVSKANG--SKNGAKSAVKKEESDTDDSVPLRA 182

Query: 292 RMKMSAAVNKSSGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWT 351
              +S    KS      +   AS++  ++    +   +  K+  S  +     D  +KWT
Sbjct: 183 VSTVSLTPYKSE-----LPSGASTTQNRSPNDEEDEDEDYKWWTSENI-----DDTQKWT 242

Query: 352 TLVHNGVIFPPPYQ--THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENF 411
           TL HNGVIF PPY+     VK+++ G PV+L PE EEVA  +A M +TD+ + P F++NF
Sbjct: 243 TLEHNGVIFAPPYEPLPKNVKLIYDGNPVNLPPEAEEVAGFYAAMLETDHAKNPVFQDNF 302

Query: 412 WNDWRKLLGK---NHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEK 471
           + D+ K+  +   NH I+    CDFT ++  ++ ++E+KK M  E+KKA+K++K ++EEK
Sbjct: 303 FRDFLKVCDECNFNHNIKEFSKCDFTQMFHHFEQKREEKKSMPKEQKKAIKQKKDEEEEK 362

Query: 472 YMWAILDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPI 531
           Y W ILDG KEKVGNFR+EPPGLFRGRG HPK G LK+R+ P  ITINIG+  P+PE P+
Sbjct: 363 YKWCILDGRKEKVGNFRIEPPGLFRGRGSHPKTGSLKRRVYPEQITINIGEGVPVPE-PL 422

Query: 532 PGQRWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIK 591
           PG +W EV+HDNTVTWLA W++ IN    KYVFLAA SSLKGQSD +KYEK+R LKDYI 
Sbjct: 423 PGHQWAEVKHDNTVTWLATWHENIN-NNVKYVFLAAGSSLKGQSDLKKYEKSRKLKDYID 482

Query: 592 NIRNSYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVP 651
           +IR  Y KD  S+    RQ   A YLID  ALRAGNEK +DEADTVGCC+L+ E+V   P
Sbjct: 483 DIRKGYRKDLKSELTVERQRGTAMYLIDVFALRAGNEKGEDEADTVGCCSLRYEHVTLKP 542

Query: 652 PNLLEFNFLGKDSIKYENTVEVELPVFKAITQF-QSGKSGSDDLFDKLDTSKLNAHLKDL 711
           P  + F+FLGKDSI+Y N VEV+  VFK +  F +  K   D +FD+L T+ LN +L  L
Sbjct: 543 PRTVVFDFLGKDSIRYYNEVEVDPQVFKNLKIFKRPPKKEGDLIFDRLSTNSLNKYLTSL 602

Query: 712 MPGLTAKVFRTYNASITLDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHG 771
           M GL+AKVFRTYNAS T+ + L +  K+  +++KI+ Y  AN+ VAI+CNHQR+V+K+H 
Sbjct: 603 MDGLSAKVFRTYNASYTMAEELKKMPKNLTLADKILFYNRANRTVAILCNHQRSVTKNHD 662

Query: 772 AQMTRLNEKISEL---RDILKELKIDLD-RAKKGKPP----------------------- 831
            QM R  E+I  L   R  L+++ ++L+ +  K KP                        
Sbjct: 663 VQMERFAERIKALQYQRMRLRKMMLNLEPKLAKSKPELLAKEEGITDSWIVKHHETLYEL 722

Query: 832 ---------------------------------LKGSDGKQK--------RNLTP----- 887
                                            LK +D  +K        + + P     
Sbjct: 723 EKEKIKKKFDRENEKLAAEDPKSVLPESELEVRLKAADELKKALDAELKSKKVDPGRSSM 782

BLAST of Csor.00g031160 vs. NCBI nr
Match: KAG6577484.1 (DNA topoisomerase 1 alpha, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2196 bits (5690), Expect = 0.0
Identity = 1140/1140 (100.00%), Postives = 1140/1140 (100.00%), Query Frame = 0

Query: 1    MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
            MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1    MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61   ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
            ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61   ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120

Query: 121  ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180
            ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD
Sbjct: 121  ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180

Query: 181  EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
            EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA
Sbjct: 181  EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240

Query: 241  DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
            DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241  DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300

Query: 301  KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
            KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301  KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360

Query: 361  THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
            THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361  THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420

Query: 421  LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
            LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421  LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480

Query: 481  PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
            PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481  PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540

Query: 541  WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
            WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541  WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600

Query: 601  IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
            IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601  IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660

Query: 661  VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
            VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661  VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720

Query: 721  MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
            MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL
Sbjct: 721  MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780

Query: 781  KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
            KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781  KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840

Query: 841  TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKKRRSFNGVSSIPFPGPSTKNIQRRPR 900
            TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKKRRSFNGVSSIPFPGPSTKNIQRRPR
Sbjct: 841  TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKKRRSFNGVSSIPFPGPSTKNIQRRPR 900

Query: 901  EFDSLSRRGQYDVDLIHLKLCFISSSHRLVMASSDLWLKEYNEASKLGDDINGMISERSS 960
            EFDSLSRRGQYDVDLIHLKLCFISSSHRLVMASSDLWLKEYNEASKLGDDINGMISERSS
Sbjct: 901  EFDSLSRRGQYDVDLIHLKLCFISSSHRLVMASSDLWLKEYNEASKLGDDINGMISERSS 960

Query: 961  VPATGPEAQRHASAIRRKITILGTKVDGLESLLLKLPVKQPLSEKEINRRKDMLAQMRSK 1020
            VPATGPEAQRHASAIRRKITILGTKVDGLESLLLKLPVKQPLSEKEINRRKDMLAQMRSK
Sbjct: 961  VPATGPEAQRHASAIRRKITILGTKVDGLESLLLKLPVKQPLSEKEINRRKDMLAQMRSK 1020

Query: 1021 VNQMASTLNMSNFANRNSLLGPDMKSADVMSKTAELDNQGLVGFQRQIMKEQDEGLEKLE 1080
            VNQMASTLNMSNFANRNSLLGPDMKSADVMSKTAELDNQGLVGFQRQIMKEQDEGLEKLE
Sbjct: 1021 VNQMASTLNMSNFANRNSLLGPDMKSADVMSKTAELDNQGLVGFQRQIMKEQDEGLEKLE 1080

Query: 1081 ETIRSTKHIALAVNEELDLHTRLIVRVLQDLMDFLVYLHNSIMVDLQGALGTLASYLHHS 1140
            ETIRSTKHIALAVNEELDLHTRLIVRVLQDLMDFLVYLHNSIMVDLQGALGTLASYLHHS
Sbjct: 1081 ETIRSTKHIALAVNEELDLHTRLIVRVLQDLMDFLVYLHNSIMVDLQGALGTLASYLHHS 1140

BLAST of Csor.00g031160 vs. NCBI nr
Match: KAG7015549.1 (DNA topoisomerase 1 beta [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1692 bits (4381), Expect = 0.0
Identity = 872/874 (99.77%), Postives = 873/874 (99.89%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
           ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120

Query: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180
           ANAA+KEENDSIHPTQENSDSEDDKPLSIRLKGNSNHP KGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 ANAALKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180

Query: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
           EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240

Query: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
           DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300

Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
           KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360

Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
           THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420

Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
           LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480

Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
           PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540

Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
           WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600

Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
           IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660

Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
           VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720

Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
           MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780

Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
           KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840

Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
           TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874

BLAST of Csor.00g031160 vs. NCBI nr
Match: XP_022932254.1 (DNA topoisomerase 1 beta-like [Cucurbita moschata] >XP_022932255.1 DNA topoisomerase 1 beta-like [Cucurbita moschata] >XP_022932256.1 DNA topoisomerase 1 beta-like [Cucurbita moschata] >XP_022932257.1 DNA topoisomerase 1 beta-like [Cucurbita moschata])

HSP 1 Score: 1685 bits (4363), Expect = 0.0
Identity = 870/874 (99.54%), Postives = 870/874 (99.54%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
           ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120

Query: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180
           ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHP KGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180

Query: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
           EVPLSSKFQMKSASGVLGGKQHGF EKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240

Query: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
           DSHSSSQIS KKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300

Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
           KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360

Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
           THGVKILHKGKPVDLT EQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420

Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
           LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480

Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
           PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540

Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
           WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600

Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
           IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660

Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
           VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720

Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
           MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780

Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
           KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840

Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
           TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874

BLAST of Csor.00g031160 vs. NCBI nr
Match: XP_023553297.1 (DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1684 bits (4362), Expect = 0.0
Identity = 867/874 (99.20%), Postives = 871/874 (99.66%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
           ANGQSSNAQKRMTSLPKTSPMRPEIGSQKA+KSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120

Query: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180
           ANAAVKEENDSIHPTQ+NSDSEDDKPLSIRLKGNSNHP KGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 ANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180

Query: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
           EVPLSSKFQMKSASGVLGG+QHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240

Query: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
           DSHSSSQIS KKPKLSDSSTPLSTNTVSLKAKQEV DDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMSAAVNKSSGS 300

Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
           KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360

Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
           THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420

Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
           LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480

Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
           PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540

Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
           WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600

Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
           IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660

Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
           VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720

Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
           MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+KEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKEL 780

Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
           KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840

Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
           TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874

BLAST of Csor.00g031160 vs. NCBI nr
Match: XP_023007686.1 (DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023007687.1 DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023007688.1 DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023007689.1 DNA topoisomerase 1 beta-like [Cucurbita maxima])

HSP 1 Score: 1675 bits (4339), Expect = 0.0
Identity = 864/874 (98.86%), Postives = 867/874 (99.20%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKA MFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1   MAVETFGKATMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
            NGQSSNAQKRMTSLPKTSPMRPEIGSQKA+KSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61  TNGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120

Query: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180
            NAAVKEENDSIHP QENSDSEDDKPLSIRLKGNSNHP KGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 VNAAVKEENDSIHPAQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180

Query: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
           EVPLSSKF+MKSASGVLGGKQHGFDEKKPLDSKVHQNGSTS DKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKPAVVSSKRPLPNA 240

Query: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
           DSHSSSQIS KKPKLSDSSTPLST+TVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300

Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
           KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360

Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
           THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420

Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
           LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480

Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
           PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540

Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
           WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600

Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
           IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660

Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
           VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720

Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
           MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780

Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
           KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840

Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
           TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874

BLAST of Csor.00g031160 vs. ExPASy TrEMBL
Match: A0A6J1EWI0 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1)

HSP 1 Score: 1685 bits (4363), Expect = 0.0
Identity = 870/874 (99.54%), Postives = 870/874 (99.54%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
           ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120

Query: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180
           ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHP KGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180

Query: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
           EVPLSSKFQMKSASGVLGGKQHGF EKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240

Query: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
           DSHSSSQIS KKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300

Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
           KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360

Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
           THGVKILHKGKPVDLT EQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420

Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
           LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480

Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
           PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540

Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
           WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600

Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
           IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660

Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
           VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720

Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
           MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780

Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
           KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840

Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
           TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874

BLAST of Csor.00g031160 vs. ExPASy TrEMBL
Match: A0A6J1KZE0 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1)

HSP 1 Score: 1675 bits (4339), Expect = 0.0
Identity = 864/874 (98.86%), Postives = 867/874 (99.20%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKA MFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1   MAVETFGKATMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
            NGQSSNAQKRMTSLPKTSPMRPEIGSQKA+KSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61  TNGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120

Query: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180
            NAAVKEENDSIHP QENSDSEDDKPLSIRLKGNSNHP KGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 VNAAVKEENDSIHPAQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180

Query: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
           EVPLSSKF+MKSASGVLGGKQHGFDEKKPLDSKVHQNGSTS DKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKPAVVSSKRPLPNA 240

Query: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300
           DSHSSSQIS KKPKLSDSSTPLST+TVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300

Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
           KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360

Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
           THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420

Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
           LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480

Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
           PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540

Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
           WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600

Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
           IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660

Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
           VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720

Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780
           MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780

Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
           KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840

Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
           TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874

BLAST of Csor.00g031160 vs. ExPASy TrEMBL
Match: A0A1S3BM72 (DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1)

HSP 1 Score: 1550 bits (4013), Expect = 0.0
Identity = 804/876 (91.78%), Postives = 831/876 (94.86%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQL+SE+KHSQ+QRLN+L GR GSD CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSS-IQEPLVKSLGTTLKVSDSMDSRPKQ 120
            NGQSSN QKRMTSLPKTSPMR EIGSQKA KSS IQEPL KSLG +LK SDSMDSRPK 
Sbjct: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPKN 120

Query: 121 LANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSD 180
           L NAAVKEEN SI+P+QENSDSEDDKPLSIRLKGNSN P KG + T+ KNK+KKSS DSD
Sbjct: 121 LVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDSD 180

Query: 181 DEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240
           DEVPLSSKFQMK ASG +G KQ GFDEKKPL  KVHQNGSTSRDKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240

Query: 241 ADSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMS-AAVNKSS 300
            DSHS SQ S KKPKLSDSS+PLS+  VSLKAKQEV DDDD IPISQR+K + A VNKSS
Sbjct: 241 EDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKSS 300

Query: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
            SK TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVK+L+KGKPVDLTP+QEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540

Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600
           AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
           DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 876

BLAST of Csor.00g031160 vs. ExPASy TrEMBL
Match: A0A0A0L2W2 (DNA topoisomerase I OS=Cucumis sativus OX=3659 GN=Csa_3G063680 PE=3 SV=1)

HSP 1 Score: 1540 bits (3987), Expect = 0.0
Identity = 798/876 (91.10%), Postives = 828/876 (94.52%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKAPMFDDDDEDNMPIVFKR+SASRQNQL+SE+KHSQ+QRL++L GR GSD CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRTSASRQNQLDSEIKHSQAQRLSKLPGRQGSDLCS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSS-IQEPLVKSLGTTLKVSDSMDSRPKQ 120
            NGQSSN QKRMTSLPKTSPMR EIGSQKA++SS I EPL KSLGT+LK S SMDSRPK 
Sbjct: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKASRSSPIPEPLTKSLGTSLKASHSMDSRPKS 120

Query: 121 LANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSD 180
           L NA VKEEN SI+P+QENSDSEDDKPLSIRLKGN N P KG + TV KNK+KKS  DSD
Sbjct: 121 LVNAVVKEENVSINPSQENSDSEDDKPLSIRLKGNPNQPNKGLVSTVPKNKVKKSLADSD 180

Query: 181 DEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240
           DEVPLSSKFQMK ASGVLG KQHGFDEKKPL  KVHQNGSTSRDKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKHASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240

Query: 241 ADSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMK-MSAAVNKSS 300
            DSHS SQ   KKPKL+DSS+PL++  VSLKAKQEV DDDD IPISQRMK +SA VNKSS
Sbjct: 241 EDSHSPSQFPTKKPKLTDSSSPLNSKLVSLKAKQEVDDDDDDIPISQRMKKVSATVNKSS 300

Query: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
            SK T+SKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKPTISKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVK+L+KGKPVDLTP QEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKMLYKGKPVDLTPPQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKA+KEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKAVKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RI PSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRICPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540

Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600
           AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
           DDMLNRGTK+GEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK
Sbjct: 721 DDMLNRGTKNGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 876

BLAST of Csor.00g031160 vs. ExPASy TrEMBL
Match: A0A5A7VHI9 (DNA topoisomerase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G002170 PE=3 SV=1)

HSP 1 Score: 1539 bits (3984), Expect = 0.0
Identity = 804/894 (89.93%), Postives = 831/894 (92.95%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQL+SE+KHSQ+QRLN+L GR GSD CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSS-IQEPLVKSLGTTLKVSDSMDSRPKQ 120
            NGQSSN QKRMTSLPKTSPMR EIGSQKA KSS IQEPL KSLG +LK SDSMDSRPK 
Sbjct: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPKN 120

Query: 121 LANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSD 180
           L NAAVKEEN SI+P+QENSDSEDDKPLSIRLKGNSN P KG + T+ KNK+KKSS DSD
Sbjct: 121 LVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDSD 180

Query: 181 DEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240
           DEVPLSSKFQMK ASG +G KQ GFDEKKPL  KVHQNGSTSRDKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240

Query: 241 ADSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMS-AAVNKSS 300
            DSHS SQ S KKPKLSDSS+PLS+  VSLKAKQEV DDDD IPISQR+K + A VNKSS
Sbjct: 241 EDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKSS 300

Query: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
            SK TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVK+L+KGKPVDLTP+QEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540

Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600
           AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
           DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEK------------------IFNKSLLAK 874
           LGTSKINYLDPRITVAWCKRHEVPIEK                  IFNKSLLAK
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKFKNFLLIFVIHNHNLLPQIFNKSLLAK 894

BLAST of Csor.00g031160 vs. TAIR 10
Match: AT5G55310.1 (DNA topoisomerase 1 beta )

HSP 1 Score: 1000.0 bits (2584), Expect = 1.6e-291
Identity = 559/931 (60.04%), Postives = 678/931 (72.82%), Query Frame = 0

Query: 1   MAVETFGKAPM------FDDDDEDNMPIVFKRSS-----ASRQNQLNSEVKHSQSQRLNQ 60
           MA E F K  +      ++D+DED++P+VFKR+S      +R + +N+ +++S       
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAI----- 60

Query: 61  LSGRPGSDACSANGQS--SNAQKRMTSLPKTSPMRPEI-GSQKAAKSSIQEPLVKSLGTT 120
                GS   S   +S  ++  +  +S  ++S M+P +  S    +S+++ PL       
Sbjct: 61  -----GSTKSSPPMRSPLTSPNRSASSSTRSSMMKPALPSSSSVQRSTLKSPLRDDRSVV 120

Query: 121 LKVSDSMDSRPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPT- 180
            K  +     P      +V + +D         DSEDDKPLS RLK +S    K P  + 
Sbjct: 121 AKERNGFGKAP------SVSKSDD--------EDSEDDKPLSARLKLDSKEVTKQPSSSG 180

Query: 181 -------VQKNKIKKSSEDS-------DDEVPLSSKFQMKSASGVLGGKQHGFDEKKPLD 240
                  VQK+ ++             D+  P+SS  Q K++ G    K    ++K+PL 
Sbjct: 181 RGSTQQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLV 240

Query: 241 SKVHQNG---STSRDKLQKPAVVSSKRPLPNADSHSSSQISLKKPKLS------------ 300
           + + +NG    T     Q PA    KRPL      SS+Q S+K+PKLS            
Sbjct: 241 NNIDRNGLKPKTEGHSSQAPA----KRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGS 300

Query: 301 -------------DSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGSKKT 360
                        D+S PL  N  ++  K++  D DDH+PI+ RMK  ++ NKSS +K +
Sbjct: 301 HISATQDAKGKNLDASKPLRANQATV--KEDNSDGDDHVPIASRMKSDSSNNKSSSAKPS 360

Query: 361 VSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHG 420
            SK+ +SS +   +K  K VK SKY KS+K LP SGDGQKKW TL HNGVIFPPPY+ HG
Sbjct: 361 SSKMIASSSRTIAQKPNKWVKDSKYSKSSKSLP-SGDGQKKWKTLQHNGVIFPPPYKRHG 420

Query: 421 VKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 480
           VKIL +GKPVDLTPEQEEVATMFAVM++T+Y +KPKF+ENFWNDWRKLLGKNH+I++LDD
Sbjct: 421 VKILFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDD 480

Query: 481 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPG 540
           CDFTPIY+WY  EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E++GNFRVEPPG
Sbjct: 481 CDFTPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPG 540

Query: 541 LFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWND 600
           LFRGRGEHPKMGKLKKRIRP DITINIGK+APIPECPIPG+RWKEV+HDNTVTWLAFW+D
Sbjct: 541 LFRGRGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSD 600

Query: 601 PINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAV 660
           PINPKEFKYVFLAASSSLKGQSDKEKYEKAR L ++I +IR +YTKDF +KD T+RQIAV
Sbjct: 601 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAV 660

Query: 661 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 720
           ATYLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEV
Sbjct: 661 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEV 720

Query: 721 ELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 780
           E  V+KAI QFQ+GKS +DDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML+
Sbjct: 721 EPLVYKAIGQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLS 780

Query: 781 RGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKID 840
           + T+DG+V EK+V YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL ID
Sbjct: 781 KETRDGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNID 840

Query: 841 LDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSK 875
           LDRAKKG+ PL GSDGK+KRNLTPEALEKKI QT  KIEK+ERDM+TKED+KTVALGTSK
Sbjct: 841 LDRAKKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSK 898

BLAST of Csor.00g031160 vs. TAIR 10
Match: AT5G55300.1 (DNA topoisomerase I alpha )

HSP 1 Score: 992.3 bits (2564), Expect = 3.3e-289
Identity = 558/927 (60.19%), Postives = 671/927 (72.38%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLNSEVKHSQSQRLNQLSGRPG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    ++S    
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60

Query: 61  SDACSANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDS 120
           S   S NG         T   KTS ++  + S  +  S  + PL   + +T+K    +  
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120

Query: 121 RPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGN----SNHPIKGPIPTVQKN-- 180
                       + D      E+ DSEDD+PLS  L GN    S+  +  P P  + N  
Sbjct: 121 ------------QKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 -KIKKSS----EDSDDEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDK 240
             + ++S    ++SDDE P+SS F+ K  SG+ GG Q   DEKKPL  K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVVSSKRPLPNADSHSSSQISLKKPKLSDSSTPLSTNTVS---------------- 300
           +    V+  KRPL    + S+ Q SLKK K+S S T +     S                
Sbjct: 241 VPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPK 300

Query: 301 LKAKQEVV-------DDDDHIPISQRMKMSAAVNKSSGSKKTVSKVASSS---------- 360
           +KAKQ          DDDD +PIS+R K  ++ + +S +K    K+ S+S          
Sbjct: 301 MKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNV 360

Query: 361 ----IKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHGVKIL 420
                +   K +KK  K SKY  S+K  PSSGDGQKKWTTLVHNGVIFPPPY+ HG+KIL
Sbjct: 361 VSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKIL 420

Query: 421 HKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
           +KGKPVDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDCDFT
Sbjct: 421 YKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFT 480

Query: 481 PIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRG 540
           PIY+W+  EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGLFRG
Sbjct: 481 PIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRG 540

Query: 541 RGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINP 600
           RGEHPKMGKLKKRI P +IT+NIGK APIPECPI G+RWKEV+HDNTVTWLAFW DPINP
Sbjct: 541 RGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINP 600

Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYL 660
           KEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR +YTK+FT+KD   RQIAVATYL
Sbjct: 601 KEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYL 660

Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
           IDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLV 720

Query: 721 FKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
           +KAI QFQ+GKS +DDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TK
Sbjct: 721 YKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETK 780

Query: 781 DGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
           DG+V++KIV YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LDRA
Sbjct: 781 DGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRA 840

Query: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 875
           KKGKPPL+GSDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYL 900

BLAST of Csor.00g031160 vs. TAIR 10
Match: AT5G55300.2 (DNA topoisomerase I alpha )

HSP 1 Score: 992.3 bits (2564), Expect = 3.3e-289
Identity = 558/927 (60.19%), Postives = 671/927 (72.38%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLNSEVKHSQSQRLNQLSGRPG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    ++S    
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60

Query: 61  SDACSANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDS 120
           S   S NG         T   KTS ++  + S  +  S  + PL   + +T+K    +  
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120

Query: 121 RPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGN----SNHPIKGPIPTVQKN-- 180
                       + D      E+ DSEDD+PLS  L GN    S+  +  P P  + N  
Sbjct: 121 ------------QKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 -KIKKSS----EDSDDEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDK 240
             + ++S    ++SDDE P+SS F+ K  SG+ GG Q   DEKKPL  K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVVSSKRPLPNADSHSSSQISLKKPKLSDSSTPLSTNTVS---------------- 300
           +    V+  KRPL    + S+ Q SLKK K+S S T +     S                
Sbjct: 241 VPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPK 300

Query: 301 LKAKQEVV-------DDDDHIPISQRMKMSAAVNKSSGSKKTVSKVASSS---------- 360
           +KAKQ          DDDD +PIS+R K  ++ + +S +K    K+ S+S          
Sbjct: 301 MKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNV 360

Query: 361 ----IKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHGVKIL 420
                +   K +KK  K SKY  S+K  PSSGDGQKKWTTLVHNGVIFPPPY+ HG+KIL
Sbjct: 361 VSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKIL 420

Query: 421 HKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
           +KGKPVDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDCDFT
Sbjct: 421 YKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFT 480

Query: 481 PIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRG 540
           PIY+W+  EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGLFRG
Sbjct: 481 PIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRG 540

Query: 541 RGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINP 600
           RGEHPKMGKLKKRI P +IT+NIGK APIPECPI G+RWKEV+HDNTVTWLAFW DPINP
Sbjct: 541 RGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINP 600

Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYL 660
           KEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR +YTK+FT+KD   RQIAVATYL
Sbjct: 601 KEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYL 660

Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
           IDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLV 720

Query: 721 FKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
           +KAI QFQ+GKS +DDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TK
Sbjct: 721 YKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETK 780

Query: 781 DGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
           DG+V++KIV YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LDRA
Sbjct: 781 DGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRA 840

Query: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 875
           KKGKPPL+GSDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYL 900

BLAST of Csor.00g031160 vs. TAIR 10
Match: AT5G55300.3 (DNA topoisomerase I alpha )

HSP 1 Score: 400.2 bits (1027), Expect = 5.5e-111
Identity = 210/319 (65.83%), Postives = 252/319 (79.00%), Query Frame = 0

Query: 556 AASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYLIDKLALRA 615
           A  SSLK           +M +D +K   +   +   S     +Q++      D      
Sbjct: 234 ADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPKMKAKQLSTREDGTDD----D 293

Query: 616 GNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQ 675
            ++ DDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+KAI QFQ
Sbjct: 294 DDDDDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQ 353

Query: 676 SGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGEVSEKI 735
           +GKS +DDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDG+V++KI
Sbjct: 354 AGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKI 413

Query: 736 VTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPLK 795
           V YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LDRAKKGKPPL+
Sbjct: 414 VVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLE 473

Query: 796 GSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAW 855
           GSDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAW
Sbjct: 474 GSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAW 533

Query: 856 CKRHEVPIEKIFNKSLLAK 875
           CKRHEVPIEKIF KSLL K
Sbjct: 534 CKRHEVPIEKIFTKSLLEK 548


HSP 2 Score: 94.4 bits (233), Expect = 6.5e-19
Identity = 101/316 (31.96%), Postives = 154/316 (48.73%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLNSEVKHSQSQRLNQLSGRPG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    ++S    
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60

Query: 61  SDACSANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDS 120
           S   S NG         T   KTS ++  + S  +  S  + PL   + +T+K    +  
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120

Query: 121 RPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGN----SNHPIKGPIPTVQKN-- 180
                       + D      E+ DSEDD+PLS  L GN    S+  +  P P  + N  
Sbjct: 121 ------------QKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 -KIKKSS----EDSDDEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDK 240
             + ++S    ++SDDE P+SS F+ K  SG+ GG Q   DEKKPL  K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVVSSKRPLPNADSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPI 300
           +    V+  KRPL    + S+ Q SLKK K+S S T +     S+K +   +DD   + +
Sbjct: 241 VPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKE---IDDKGRVLV 286

BLAST of Csor.00g031160 vs. TAIR 10
Match: AT1G79590.1 (syntaxin of plants 52 )

HSP 1 Score: 232.6 bits (592), Expect = 1.5e-60
Identity = 125/202 (61.88%), Postives = 162/202 (80.20%), Query Frame = 0

Query: 932  ASSDLWLKEYNEASKLGDDINGMISERSSVPATGPEAQRHASAIRRKITILGTKVDGLES 991
            +SSD W++EYNEA KL +DINGM+SER++   TGP+AQR ASAIRRKITILGT++D L+S
Sbjct: 3    SSSDPWMREYNEALKLSEDINGMMSERNASGLTGPDAQRRASAIRRKITILGTRLDSLQS 62

Query: 992  LLLKLPVKQPLSEKEINRRKDMLAQMRSKVNQMASTLNMSNFANRNSLLGPDMKSADVMS 1051
            LL+K+P KQ +SEKE+NRRKDM+  +RSK NQ+AS LNMSNFANR+SL G D+K  D ++
Sbjct: 63   LLVKVPGKQHVSEKEMNRRKDMVGNLRSKTNQVASALNMSNFANRDSLFGTDLKPDDAIN 122

Query: 1052 KTAELDNQGLVGFQRQIMKEQDEGLEKLEETIRSTKHIALAVNEELDLHTRLIVRVLQDL 1111
            + + +DNQG+V FQRQ+M+EQDEGLEKLEET+ STKHIALAVNEEL L TRLI     D 
Sbjct: 123  RVSGMDNQGIVVFQRQVMREQDEGLEKLEETVMSTKHIALAVNEELTLQTRLI-----DD 182

Query: 1112 MDFLVYLHNSIMVDLQGALGTL 1134
            +D+ V + +S +  +Q +L  +
Sbjct: 183  LDYDVDITDSRLRRVQKSLALM 199

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJ792.2e-29060.04DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1[more]
P301814.6e-28860.19DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1[more]
P931191.1e-23360.10DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1[more]
Q54RC35.7e-14539.08DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1[more]
P077991.3e-14139.81DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28... [more]
Match NameE-valueIdentityDescription
KAG6577484.10.0100.00DNA topoisomerase 1 alpha, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7015549.10.099.77DNA topoisomerase 1 beta [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022932254.10.099.54DNA topoisomerase 1 beta-like [Cucurbita moschata] >XP_022932255.1 DNA topoisome... [more]
XP_023553297.10.099.20DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo][more]
XP_023007686.10.098.86DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023007687.1 DNA topoisomera... [more]
Match NameE-valueIdentityDescription
A0A6J1EWI00.099.54DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1[more]
A0A6J1KZE00.098.86DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1[more]
A0A1S3BM720.091.78DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1[more]
A0A0A0L2W20.091.10DNA topoisomerase I OS=Cucumis sativus OX=3659 GN=Csa_3G063680 PE=3 SV=1[more]
A0A5A7VHI90.089.93DNA topoisomerase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G... [more]
Match NameE-valueIdentityDescription
AT5G55310.11.6e-29160.04DNA topoisomerase 1 beta [more]
AT5G55300.13.3e-28960.19DNA topoisomerase I alpha [more]
AT5G55300.23.3e-28960.19DNA topoisomerase I alpha [more]
AT5G55300.35.5e-11165.83DNA topoisomerase I alpha [more]
AT1G79590.11.5e-6061.88syntaxin of plants 52 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 433..461
NoneNo IPR availableCOILSCoilCoilcoord: 763..790
NoneNo IPR availableCOILSCoilCoilcoord: 814..834
NoneNo IPR availableCOILSCoilCoilcoord: 1076..1096
NoneNo IPR availableGENE3D1.20.5.110coord: 1068..1114
e-value: 6.1E-8
score: 34.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 290..312
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..76
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 290..323
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 201..220
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..266
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..117
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 788..807
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 170..184
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 130..150
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 237..266
NoneNo IPR availablePANTHERPTHR10290DNA TOPOISOMERASE Icoord: 122..875
NoneNo IPR availablePANTHERPTHR10290:SF17DNA TOPOISOMERASE Icoord: 122..875
NoneNo IPR availableCDDcd00660Topoisomer_IB_Ncoord: 344..556
e-value: 7.4351E-120
score: 366.204
NoneNo IPR availableCDDcd15841SNARE_Qccoord: 1069..1104
e-value: 8.62003E-9
score: 50.6348
NoneNo IPR availableSUPERFAMILY58038SNARE fusion complexcoord: 1057..1109
IPR001631DNA topoisomerase IPRINTSPR00416EUTPISMRASEIcoord: 645..661
score: 57.14
coord: 484..493
score: 71.07
coord: 623..637
score: 66.19
coord: 703..717
score: 80.0
coord: 600..619
score: 67.14
coord: 840..851
score: 72.62
IPR013499DNA topoisomerase I, eukaryotic-typeSMARTSM00435topeucoord: 484..860
e-value: 1.1E-265
score: 898.5
IPR013034DNA topoisomerase I, DNA binding, N-terminal domain 1GENE3D1.10.10.41coord: 360..443
e-value: 1.7E-95
score: 320.7
IPR014711DNA topoisomerase I, catalytic core, alpha-helical subdomain, eukaryotic-typeGENE3D3.90.15.10Topoisomerase I; Chain A, domain 3coord: 558..706
e-value: 1.1E-56
score: 193.2
IPR025834Topoisomerase I C-terminal domainPFAMPF14370Topo_C_assoccoord: 819..874
e-value: 4.7E-27
score: 93.7
IPR014727DNA topoisomerase I, catalytic core, alpha/beta subdomainGENE3D1.10.132.10coord: 707..881
e-value: 5.1E-60
score: 204.2
IPR013500DNA topoisomerase I, catalytic core, eukaryotic-typePFAMPF01028Topoisom_Icoord: 558..781
e-value: 8.9E-92
score: 306.5
IPR013500DNA topoisomerase I, catalytic core, eukaryotic-typeCDDcd00659Topo_IB_Ccoord: 564..757
e-value: 6.40251E-78
score: 252.968
IPR008336DNA topoisomerase I, DNA binding, eukaryotic-typePFAMPF02919Topoisom_I_Ncoord: 344..555
e-value: 1.5E-93
score: 312.2
IPR013030DNA topoisomerase I, DNA binding, N-terminal domain 2GENE3D2.170.11.10DNA Topoisomerase I, domain 2coord: 340..555
e-value: 1.7E-95
score: 320.7
IPR018521DNA topoisomerase I, active sitePROSITEPS00176TOPOISOMERASE_I_EUKcoord: 833..851
IPR036202DNA topoisomerase I, DNA binding, eukaryotic-type, N-terminal domain superfamilySUPERFAMILY56741Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragmentcoord: 339..556
IPR011010DNA breaking-rejoining enzyme, catalytic coreSUPERFAMILY56349DNA breaking-rejoining enzymescoord: 558..875

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g031160.m01Csor.00g031160.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006338 chromatin remodeling
biological_process GO:0007059 chromosome segregation
biological_process GO:0006260 DNA replication
biological_process GO:0006265 DNA topological change
cellular_component GO:0005694 chromosome
cellular_component GO:0005730 nucleolus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity