Csor.00g031080 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g031080
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPectin lyase-like superfamily protein
LocationCsor_Chr16: 6671966 .. 6673725 (+)
RNA-Seq ExpressionCsor.00g031080
SyntenyCsor.00g031080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTTGAATTCATGTTTAGTGTTCTTTATCATCTTACTTTCCATTCCTAAATGTTATTCTCAATCTAAAAAGTTCAATATCATCCAATTTGGTGCAACAGCGGATGGAAAAACGGATAGCACCGAGGTAAAATTTTTCTTTCTAATAAACCATAAAAACATCAAAACTCAACTCAAACAACCTTTTGTGATGTATTCACATACTCTTAGGTAACATTTTTATTTTTCATTTATTTTATTTTATTTTTTTTAGTTTTTAAAAAATAGAATCATTAGAAGCATTACTTTAGAAATACCATTTTCAAAATGGGATACAATTTTTTTTTTCTTTAATATCTATTTCATGTCTACAAATATCTAAACAGAATCTTTGAAATTAAAAAAATATATATATTTTATGTGTTTGGATTATTCTTCCTAATAAATATCTCAATGTAAATTTAAAATTTAAGTTATTTTGTTGAAAGTACCTCATAAAAGGTTACTTGGTTTGTATAGGCATTAAGTAGGGCATGGAAGGAAGCTTGTGGACATAACGGACATGGTATAGTTTTAGTACCAGCGGGGAGCTTTTTGGTTTTACCGTTAGTGCTTGAAGGTCCATGCAAAGGATCCATTGGTCTGCTTATTAATGGTAAATTGTTGGCTCCTGTAAGCAAGAATTTCGCCATCGGTAAATACTGGTTGAGTATCTCCAAAGTCGACAACTTTTTCGTTAACGGTTCTGGCAGCTTGAACGGCGGCGGTTCTTCAGCTTGGCATTCCGACACTTCTAACCGTCCAATTGTAAGTCCATTTTTATATTTAAGTGATAAAAAAGAAATTGATTTGTTAGGTTTATAGTTAAATGATGCTATCATTTTACATTTAAAAGTATACCAAAATATACATATGTATAATCTAATCTAACGTTTGATTCAATGTAGTCGATGAAACTTTCTGGTGTCAACACTGCGAGAATTTCAAAGATAACATCGTCCAATAGCAAATTCTTCCATTTTGGTGTGCATAAATGTCGTGATGTCACGTTTGATCACGTTACAATCATAGCTCCAGCAGATAGCCCCAATACAGATGGAATTCACATTTCCAAGTCAAGTGGTATCACTATTATGCATTCAGTGATTCGCACCGGAGACGATTGCATCTCTCTAGGCCCTGGAAGTAAATTCATCAACATAACAGACGTCCAGTGTGGTCCAGGTCATGGAATTAGCATCGGTAGCCTCGGAAAGTACCCAAATGAAGAAGATGTCTCTGAAGTCACAATAAGAGACTCTACATTTGTTGGCACAGACAACGGTGTGAGGATCAAAACATGGTCAACTTCATTTTCTAGTTCTGTTAGCAACATCAGTTTTCTCAATCTCGAAATGAAGGATGTAAAAAATCCTATCATCATTGATCAAAAATATTGCACTGATTGCGACGAACATAAAATGGTATATATATTCAAATTTGATAGTTGTTCGGTTTTAGTAAAACTATAAATGGTGAATGATTCTAAAGTAAAATGTGGTTTGTGGTTGACAGAGTAACTCAAAAGTACAGATTAAAGACGTGAGATATGAAGGCATAAGGGGGAGTTCAACGTCCCAAGCGGCAGTAAGTTTTGAATGCAGCCAAGTTTGTTCATGTGAAAGCATAGTGTTACAAGACATTAACCTCTCCTACAAAGGTGATGGCGAACCAACTTCCATTTGTCACAATGTGAAGGGCTCTGCATTTGGCAAACAACTTCCTTCCTCTTGCCTTTAA

mRNA sequence

ATGAGTTTGAATTCATGTTTAGTGTTCTTTATCATCTTACTTTCCATTCCTAAATGTTATTCTCAATCTAAAAAGTTCAATATCATCCAATTTGGTGCAACAGCGGATGGAAAAACGGATAGCACCGAGGCATTAAGTAGGGCATGGAAGGAAGCTTGTGGACATAACGGACATGGTATAGTTTTAGTACCAGCGGGGAGCTTTTTGGTTTTACCGTTAGTGCTTGAAGGTCCATGCAAAGGATCCATTGGTCTGCTTATTAATGGTAAATTGTTGGCTCCTGTAAGCAAGAATTTCGCCATCGGTAAATACTGGTTGAGTATCTCCAAAGTCGACAACTTTTTCGTTAACGGTTCTGGCAGCTTGAACGGCGGCGGTTCTTCAGCTTGGCATTCCGACACTTCTAACCGTCCAATTAGTAACTCAAAAGTACAGATTAAAGACGTGAGATATGAAGGCATAAGGGGGAGTTCAACGTCCCAAGCGGCAGTAAGTTTTGAATGCAGCCAAGTTTGTTCATGTGAAAGCATAGTGTTACAAGACATTAACCTCTCCTACAAAGGTGATGGCGAACCAACTTCCATTTGTCACAATGTGAAGGGCTCTGCATTTGGCAAACAACTTCCTTCCTCTTGCCTTTAA

Coding sequence (CDS)

ATGAGTTTGAATTCATGTTTAGTGTTCTTTATCATCTTACTTTCCATTCCTAAATGTTATTCTCAATCTAAAAAGTTCAATATCATCCAATTTGGTGCAACAGCGGATGGAAAAACGGATAGCACCGAGGCATTAAGTAGGGCATGGAAGGAAGCTTGTGGACATAACGGACATGGTATAGTTTTAGTACCAGCGGGGAGCTTTTTGGTTTTACCGTTAGTGCTTGAAGGTCCATGCAAAGGATCCATTGGTCTGCTTATTAATGGTAAATTGTTGGCTCCTGTAAGCAAGAATTTCGCCATCGGTAAATACTGGTTGAGTATCTCCAAAGTCGACAACTTTTTCGTTAACGGTTCTGGCAGCTTGAACGGCGGCGGTTCTTCAGCTTGGCATTCCGACACTTCTAACCGTCCAATTAGTAACTCAAAAGTACAGATTAAAGACGTGAGATATGAAGGCATAAGGGGGAGTTCAACGTCCCAAGCGGCAGTAAGTTTTGAATGCAGCCAAGTTTGTTCATGTGAAAGCATAGTGTTACAAGACATTAACCTCTCCTACAAAGGTGATGGCGAACCAACTTCCATTTGTCACAATGTGAAGGGCTCTGCATTTGGCAAACAACTTCCTTCCTCTTGCCTTTAA

Protein sequence

MSLNSCLVFFIILLSIPKCYSQSKKFNIIQFGATADGKTDSTEALSRAWKEACGHNGHGIVLVPAGSFLVLPLVLEGPCKGSIGLLINGKLLAPVSKNFAIGKYWLSISKVDNFFVNGSGSLNGGGSSAWHSDTSNRPISNSKVQIKDVRYEGIRGSSTSQAAVSFECSQVCSCESIVLQDINLSYKGDGEPTSICHNVKGSAFGKQLPSSCL
Homology
BLAST of Csor.00g031080 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.7e-21
Identity = 56/176 (31.82%), Postives = 91/176 (51.70%), Query Frame = 0

Query: 26  FNIIQFGATADGKTDSTEALSRAWKEACGHNGHGIVLVPAGSFLVLPLVLEGPCKGSIGL 85
           F+I + GA+ +GKTDST+A+  AW  ACG  G   +L+P G FLV PL   GPCKG + +
Sbjct: 41  FDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGPLNFTGPCKGDVTI 100

Query: 86  LINGKLLAPVS-KNFAIGKYWLSISKVDNFFVNGSGSLNGGGSSAWHSDTSNRPISNSKV 145
            +NG LLA      +     W+ I +VDN  + G G L+G G + W  ++  +   + K+
Sbjct: 101 QVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVKKY-DCKI 160

Query: 146 QIKDVRYEGIRGSSTSQAAV---SFECSQVCSCESIVLQDINLSYKGDGEPTSICH 198
               +  + +     S   +    F    +  C+ ++++D+N++  GD   T   H
Sbjct: 161 LPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIH 215

BLAST of Csor.00g031080 vs. ExPASy Swiss-Prot
Match: P35338 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 5.3e-20
Identity = 53/176 (30.11%), Postives = 91/176 (51.70%), Query Frame = 0

Query: 26  FNIIQFGATADGKTDSTEALSRAWKEACGHNGHGIVLVPAGSFLVLPLVLEGPCKGSIGL 85
           F+I + GA+ +GKTDST+A+  AW  ACG  G   +L+P G FLV  L   GPCKG + +
Sbjct: 41  FDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGDVTI 100

Query: 86  LINGKLLAPVS-KNFAIGKYWLSISKVDNFFVNGSGSLNGGGSSAWHSDTSNRPISNSKV 145
            ++G LLA      +     W+ I +VDN  + G G+L+G G + W  ++  +   + K+
Sbjct: 101 QVDGNLLATTDLSQYKEHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKY-DCKI 160

Query: 146 QIKDVRYEGIRGSSTSQAAV---SFECSQVCSCESIVLQDINLSYKGDGEPTSICH 198
               +  + +     S   +    F    +  C++++++D+ ++  GD   T   H
Sbjct: 161 LPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYQCKNMLIKDVTVTAPGDSPNTDGIH 215

BLAST of Csor.00g031080 vs. ExPASy Swiss-Prot
Match: P26216 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 9.1e-20
Identity = 53/176 (30.11%), Postives = 90/176 (51.14%), Query Frame = 0

Query: 26  FNIIQFGATADGKTDSTEALSRAWKEACGHNGHGIVLVPAGSFLVLPLVLEGPCKGSIGL 85
           F+I + GA+ +GKTDST+A+  AW  ACG  G   +L+P G FLV  L   GPCKG + +
Sbjct: 41  FDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGDVTI 100

Query: 86  LINGKLLAPVS-KNFAIGKYWLSISKVDNFFVNGSGSLNGGGSSAWHSDTSNRPISNSKV 145
            ++G LLA      +     W+ I +VDN  + G G+L+G G + W  ++  +   + K+
Sbjct: 101 QVDGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKY-DCKI 160

Query: 146 QIKDVRYEGIRGSSTSQAAV---SFECSQVCSCESIVLQDINLSYKGDGEPTSICH 198
               +  + +     S   +    F    +  C+ ++++D+ ++  GD   T   H
Sbjct: 161 LPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTVTAPGDSPNTDGIH 215

BLAST of Csor.00g031080 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.9e-17
Identity = 59/195 (30.26%), Postives = 102/195 (52.31%), Query Frame = 0

Query: 8   VFFIILLSIPKCYSQSKKFNII-QFGATADGKTDSTEALSRAWKEACGHNGHGIVLVPAG 67
           +F ++LL I     QS  F+++ +FGA ADGKTD ++    AWKEAC       V++P G
Sbjct: 11  MFVLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKG 70

Query: 68  SFLVLPLVLEGPCKGSIGLLINGKLLAPVSKNFAIGKYWLSISKVDNFFVNGSGSLNGGG 127
           ++L+  + LEGPCK  I + + G + AP   +      W+    V+NF + G G  +G G
Sbjct: 71  TYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQG 130

Query: 128 SSAWHSDTSNRPISNSKVQIKDVRYEGIRGS----STSQAAVSFECSQVCSCESIVLQDI 187
           S A+  +T       SK+ + ++R++ +  +     TS+ +  F  + V +C++I L+ +
Sbjct: 131 SIAYEKNTCENREFRSKLPV-NIRFDFLTNALIQDITSKDSKLFHIN-VFACKNITLERL 190

Query: 188 NLSYKGDGEPTSICH 198
            +    +   T   H
Sbjct: 191 KIEAPDESPNTDGIH 203

BLAST of Csor.00g031080 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 5.5e-17
Identity = 58/195 (29.74%), Postives = 101/195 (51.79%), Query Frame = 0

Query: 8   VFFIILLSIPKCYSQSKKFNII-QFGATADGKTDSTEALSRAWKEACGHNGHGIVLVPAG 67
           +F ++LL I     Q   F+++ +FGA ADGKTD ++    AWKEAC       V++P G
Sbjct: 11  MFVLLLLFISASKVQPDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKG 70

Query: 68  SFLVLPLVLEGPCKGSIGLLINGKLLAPVSKNFAIGKYWLSISKVDNFFVNGSGSLNGGG 127
           ++L+  + LEGPCK  I + + G + AP   +      W+    V+NF + G G  +G G
Sbjct: 71  TYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQG 130

Query: 128 SSAWHSDTSNRPISNSKVQIKDVRYEGIRGS----STSQAAVSFECSQVCSCESIVLQDI 187
           S A+  +T       SK+ + ++R++ +  +     TS+ +  F  + V +C++I L+ +
Sbjct: 131 SIAYEKNTCENREFRSKLPV-NIRFDFVTNALIQDITSKDSKLFHIN-VFACKNITLERL 190

Query: 188 NLSYKGDGEPTSICH 198
            +    +   T   H
Sbjct: 191 KIEAPDESPNTDGIH 203

BLAST of Csor.00g031080 vs. NCBI nr
Match: KAG6577492.1 (Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 425 bits (1093), Expect = 4.30e-150
Identity = 213/213 (100.00%), Postives = 213/213 (100.00%), Query Frame = 0

Query: 1   MSLNSCLVFFIILLSIPKCYSQSKKFNIIQFGATADGKTDSTEALSRAWKEACGHNGHGI 60
           MSLNSCLVFFIILLSIPKCYSQSKKFNIIQFGATADGKTDSTEALSRAWKEACGHNGHGI
Sbjct: 1   MSLNSCLVFFIILLSIPKCYSQSKKFNIIQFGATADGKTDSTEALSRAWKEACGHNGHGI 60

Query: 61  VLVPAGSFLVLPLVLEGPCKGSIGLLINGKLLAPVSKNFAIGKYWLSISKVDNFFVNGSG 120
           VLVPAGSFLVLPLVLEGPCKGSIGLLINGKLLAPVSKNFAIGKYWLSISKVDNFFVNGSG
Sbjct: 61  VLVPAGSFLVLPLVLEGPCKGSIGLLINGKLLAPVSKNFAIGKYWLSISKVDNFFVNGSG 120

Query: 121 SLNGGGSSAWHSDTSNRPISNSKVQIKDVRYEGIRGSSTSQAAVSFECSQVCSCESIVLQ 180
           SLNGGGSSAWHSDTSNRPISNSKVQIKDVRYEGIRGSSTSQAAVSFECSQVCSCESIVLQ
Sbjct: 121 SLNGGGSSAWHSDTSNRPISNSKVQIKDVRYEGIRGSSTSQAAVSFECSQVCSCESIVLQ 180

Query: 181 DINLSYKGDGEPTSICHNVKGSAFGKQLPSSCL 213
           DINLSYKGDGEPTSICHNVKGSAFGKQLPSSCL
Sbjct: 181 DINLSYKGDGEPTSICHNVKGSAFGKQLPSSCL 213

BLAST of Csor.00g031080 vs. NCBI nr
Match: KAG6577490.1 (Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 372 bits (954), Expect = 5.98e-129
Identity = 183/208 (87.98%), Postives = 196/208 (94.23%), Query Frame = 0

Query: 6   CLVFFIILLSIPKCYSQSKKFNIIQFGATADGKTDSTEALSRAWKEACGHNGHGIVLVPA 65
           C V FIILLSIPKCYSQSK+FNIIQFGATADGKT+ST+ALS AW+EACGHNGHG+VLVPA
Sbjct: 3   CSVIFIILLSIPKCYSQSKEFNIIQFGATADGKTESTKALSEAWREACGHNGHGVVLVPA 62

Query: 66  GSFLVLPLVLEGPCKGSIGLLINGKLLAPVSKNFAIGKYWLSISKVDNFFVNGSGSLNGG 125
           G FL+LPLVLEGPCK SIGLLI+G+LLAPVSKNFA G YWLSISKVDN FV+GSGSLNGG
Sbjct: 63  GRFLILPLVLEGPCKVSIGLLIHGELLAPVSKNFATGGYWLSISKVDNLFVDGSGSLNGG 122

Query: 126 GSSAWHSDTSNRPISNSKVQIKDVRYEGIRGSSTSQAAVSFECSQVCSCESIVLQDINLS 185
           GSSAW SDTSNRP+SNSKVQI+DVRYEGIRGSSTSQ AVSFECSQVCSCESIVLQDINLS
Sbjct: 123 GSSAWPSDTSNRPVSNSKVQIRDVRYEGIRGSSTSQVAVSFECSQVCSCESIVLQDINLS 182

Query: 186 YKGDGEPTSICHNVKGSAFGKQLPSSCL 213
           YKGDG+PTSICHNV+GSA GKQ PSSCL
Sbjct: 183 YKGDGQPTSICHNVQGSASGKQFPSSCL 210

BLAST of Csor.00g031080 vs. NCBI nr
Match: KAG6577493.1 (Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 369 bits (947), Expect = 8.94e-128
Identity = 187/217 (86.18%), Postives = 199/217 (91.71%), Query Frame = 0

Query: 1   MSLNS----CLVFFIILLSIPKCYSQSKKFNIIQFGATADGKTDSTEALSRAWKEACGHN 60
           MSLNS    CLVFFIILLS+PKCYSQSK+FNIIQFGA ADGKTDST+ALS AWK ACGHN
Sbjct: 1   MSLNSSDMGCLVFFIILLSVPKCYSQSKEFNIIQFGAIADGKTDSTKALSEAWKGACGHN 60

Query: 61  GHGIVLVPAGSFLVLPLVLEGPCKGSIGLLINGKLLAPVSKNFAIGKYWLSISKVDNFFV 120
           G+GIVLVP GSFLVLPLVLEGPCKGSIGL ING+LLAPVSKNFA G+YWLSIS VDNFFV
Sbjct: 61  GYGIVLVPEGSFLVLPLVLEGPCKGSIGLHINGELLAPVSKNFATGEYWLSISDVDNFFV 120

Query: 121 NGSGSLNGGGSSAWHSDTSNRPISNSKVQIKDVRYEGIRGSSTSQAAVSFECSQVCSCES 180
           +GSGSLNGGGSSAW SDTSNRPISNSKVQI+DVRYEGI+GSSTSQ AV+FECSQV  C+ 
Sbjct: 121 DGSGSLNGGGSSAWQSDTSNRPISNSKVQIRDVRYEGIKGSSTSQVAVNFECSQVLPCQG 180

Query: 181 IVLQDINLSYKGDGEPTSICHNVKGSAFGKQLPSSCL 213
           IVLQDINLS KGDGEPTSIC NVKGSAFGKQ+PSSCL
Sbjct: 181 IVLQDINLSLKGDGEPTSICDNVKGSAFGKQIPSSCL 217

BLAST of Csor.00g031080 vs. NCBI nr
Match: XP_022932248.1 (exopolygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 303 bits (776), Expect = 2.34e-99
Identity = 184/380 (48.42%), Postives = 197/380 (51.84%), Query Frame = 0

Query: 6   CLVFFIILLSIPKCYSQSKKFNIIQFGATADGKTDSTEALSRAWKEACGHNGHGIVLVPA 65
           CLVFFIILLSIPKCYSQSK+FNIIQFGATADGKT+ST+ALS AW+EACGHNGHG+VLVPA
Sbjct: 3   CLVFFIILLSIPKCYSQSKEFNIIQFGATADGKTESTKALSEAWREACGHNGHGVVLVPA 62

Query: 66  GSFLVLPLVLEGPCKGSIGLLINGKLLAPVSKNFAIGKYWLSISKVDNFFVNGSGSLNGG 125
           G FL+LPLVLEGPCK SIGLLI+G+LLAPVSKNFA G YWLSISKVDN FV+GSGSLNGG
Sbjct: 63  GRFLILPLVLEGPCKVSIGLLIHGELLAPVSKNFATGGYWLSISKVDNLFVDGSGSLNGG 122

Query: 126 GSSAWHSDTSNRPIS--------------------------------------------- 185
           GSSAW SDTSNRP+S                                             
Sbjct: 123 GSSAWPSDTSNRPVSMKLSSVNTARISKITSSNSKFFHFGVHGCRDVTFDHVTVIAPADS 182

Query: 186 ------------------------------------------------------------ 213
                                                                       
Sbjct: 183 PNTDGIHVSKSSGINIMHSVIRTGDDCISLGPGSKFINITDIQCGPGHGISIGSLGKYPN 242

BLAST of Csor.00g031080 vs. NCBI nr
Match: KAG6577489.1 (Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 278 bits (710), Expect = 1.05e-91
Identity = 141/217 (64.98%), Postives = 168/217 (77.42%), Query Frame = 0

Query: 1   MSLNS----CLVFFIILLSIPKCYSQSKKFNIIQFGATADGKTDSTEALSRAWKEACGHN 60
           M LNS    C  F  ILL IP CY+Q+  FNI+QFGA  DG+TDST+ALS+AW +AC  +
Sbjct: 1   MGLNSWHIACFAFITILLCIPNCYAQNV-FNIMQFGARNDGRTDSTQALSKAWSQACSRD 60

Query: 61  GHGIVLVPAGSFLVLPLVLEGPCKGSIGLLINGKLLAPVSKNFAIGKYWLSISKVDNFFV 120
           G  +VLVP G FL+  LVL+GPCKGSIGL ING+LLAP+  NFA+G+ WLSI++VDN FV
Sbjct: 61  GGSVVLVPDGRFLIFSLVLKGPCKGSIGLRINGELLAPIDPNFAVGQIWLSINEVDNLFV 120

Query: 121 NGSGSLNGGGSSAWHSDTSNRPISNSKVQIKDVRYEGIRGSSTSQAAVSFECSQVCSCES 180
           +G GSLNG GS AW     NRPISNS+V+I+DVRYE IRG+S +Q AV+FECSQV  CE 
Sbjct: 121 DGYGSLNGRGSLAWSIKKFNRPISNSRVKIRDVRYENIRGTSATQVAVNFECSQVLPCEG 180

Query: 181 IVLQDINLSYKGDGEPTSICHNVKGSAFGKQLPSSCL 213
           IVLQDINLS+ G G+ TSIC NVKG AFGKQ P+SCL
Sbjct: 181 IVLQDINLSFNGRGQLTSICQNVKGHAFGKQFPNSCL 216

BLAST of Csor.00g031080 vs. ExPASy TrEMBL
Match: A0A6J1F153 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111438612 PE=3 SV=1)

HSP 1 Score: 303 bits (776), Expect = 1.13e-99
Identity = 184/380 (48.42%), Postives = 197/380 (51.84%), Query Frame = 0

Query: 6   CLVFFIILLSIPKCYSQSKKFNIIQFGATADGKTDSTEALSRAWKEACGHNGHGIVLVPA 65
           CLVFFIILLSIPKCYSQSK+FNIIQFGATADGKT+ST+ALS AW+EACGHNGHG+VLVPA
Sbjct: 3   CLVFFIILLSIPKCYSQSKEFNIIQFGATADGKTESTKALSEAWREACGHNGHGVVLVPA 62

Query: 66  GSFLVLPLVLEGPCKGSIGLLINGKLLAPVSKNFAIGKYWLSISKVDNFFVNGSGSLNGG 125
           G FL+LPLVLEGPCK SIGLLI+G+LLAPVSKNFA G YWLSISKVDN FV+GSGSLNGG
Sbjct: 63  GRFLILPLVLEGPCKVSIGLLIHGELLAPVSKNFATGGYWLSISKVDNLFVDGSGSLNGG 122

Query: 126 GSSAWHSDTSNRPIS--------------------------------------------- 185
           GSSAW SDTSNRP+S                                             
Sbjct: 123 GSSAWPSDTSNRPVSMKLSSVNTARISKITSSNSKFFHFGVHGCRDVTFDHVTVIAPADS 182

Query: 186 ------------------------------------------------------------ 213
                                                                       
Sbjct: 183 PNTDGIHVSKSSGINIMHSVIRTGDDCISLGPGSKFINITDIQCGPGHGISIGSLGKYPN 242

BLAST of Csor.00g031080 vs. ExPASy TrEMBL
Match: A0A6J1L832 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111500170 PE=3 SV=1)

HSP 1 Score: 218 bits (555), Expect = 4.79e-66
Identity = 148/389 (38.05%), Postives = 174/389 (44.73%), Query Frame = 0

Query: 1   MSLNS----CLVFFIILLSIPKCYSQSKKFNIIQFGATADGKTDSTEALSRAWKEACGHN 60
           M LNS    CLV   ILL I KCY+Q+  FNI+QFGA+ DG+TDST+ALS+AW++AC  +
Sbjct: 18  MGLNSSHIECLVLIAILLCITKCYAQNV-FNIMQFGASNDGRTDSTQALSKAWRQACWRD 77

Query: 61  GHGIVLVPAGSFLVLPLVLEGPCKGSIGLLINGKLLAPVSKNFAIGKYWLSISKVDNFFV 120
           G G+VLVP G FL+  LVL+GPCKGSIGL ING+LLAP+  NFAI + WLSI +VDN FV
Sbjct: 78  GGGVVLVPDGRFLIFSLVLKGPCKGSIGLRINGQLLAPIDPNFAIAQIWLSIYEVDNLFV 137

Query: 121 NGSGSLNGGGSSAWHSDTSNRPIS------------------------------------ 180
           +G GS +G G  AW   T NRPIS                                    
Sbjct: 138 DGYGSFDGRGFLAWSIKTFNRPISMKLTKINHARITGITSYNSKFFHFGVHGCSDVTFDH 197

Query: 181 ------------------------------------------------------------ 213
                                                                       
Sbjct: 198 IRVMAPRDSPNTDGIHISKSSGINIVHSRFDTGDDCISLGPGSRFINITNIHCGPGHGIS 257

BLAST of Csor.00g031080 vs. ExPASy TrEMBL
Match: A0A6J1L5E9 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111500171 PE=3 SV=1)

HSP 1 Score: 175 bits (443), Expect = 3.60e-48
Identity = 104/206 (50.49%), Postives = 127/206 (61.65%), Query Frame = 0

Query: 1   MSLNS----CLVFFIILLSIPKCYSQSKKFNIIQFGATADGKTDSTEALSRAWKEACGHN 60
           M LNS    CLV   ILL I KCY+Q+  FNI+QFGA+ DG+TDST+ALS+AW++AC  +
Sbjct: 18  MGLNSSHIECLVLIAILLCITKCYAQNV-FNIMQFGASNDGRTDSTQALSKAWRQACWRD 77

Query: 61  GHGIVLVPAGSFLVLPLVLEGPCKGSIGLLINGKLLAPVSKNFAIGKYWLSISKVDNFFV 120
           G G+VLVP G FL+  LVL+GPCKGSIGL ING+LLAP+  NFAI + WLSI +VDN FV
Sbjct: 78  GGGVVLVPDGRFLIFSLVLKGPCKGSIGLRINGQLLAPIDPNFAIAQIWLSIYEVDNLFV 137

Query: 121 NGSGSLNGGGSSAWHSDTSNRPISNSKVQIKDVRYEGIRGSSTSQAAVSFECSQVCSCES 180
           +G GS +G G  AW   T NRPIS    +I   R  GI    TS  +  F    V  C  
Sbjct: 138 DGYGSFDGRGFLAWSIKTFNRPISMKLTKINHARITGI----TSYNSKFFHFG-VHGCSD 197

Query: 181 IVLQDINLSYKGDGEPTSICHNVKGS 202
           +    I +    D   T   H  K S
Sbjct: 198 VTFDHIRVMAPRDSPNTDGIHISKSS 217

BLAST of Csor.00g031080 vs. ExPASy TrEMBL
Match: A0A0A0L7H9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G063610 PE=3 SV=1)

HSP 1 Score: 167 bits (422), Expect = 1.16e-46
Identity = 119/360 (33.06%), Postives = 147/360 (40.83%), Query Frame = 0

Query: 29  IQFGATADGKTDSTEALSRAWKEACGHNGHGIVLVPA-GSFLVLPLVLEGPCKGSIGLLI 88
           + FGA  D  TD+++ALS AW EAC +NG GIVL+PA G FLVLPL+L+GPC G I + +
Sbjct: 1   MHFGAKPDAITDNSKALSSAWSEACTNNGGGIVLIPAKGRFLVLPLLLQGPCHGFIRIQL 60

Query: 89  NGKLLAPVSKNFAIGKYWLSISKVDNFFVNGSGSLNGGGSSAWHSDTSNRPIS------- 148
           +G+LLAP+ ++FA G YWLSI +V+N F++G GSL+G GS+AW S + NRP+S       
Sbjct: 61  DGELLAPLDEHFATGDYWLSIDQVNNLFIDGLGSLDGRGSTAWPSTSHNRPVSMRLNGIN 120

Query: 149 ----------NSK----------------------------------------------- 208
                     NSK                                               
Sbjct: 121 NARITNIKSYNSKLFHFAMHGCRDVTFDHVTVIAPANSPNTDGIHISSSSGINIMHSTIG 180

Query: 209 ------------------------------------------------------------ 213
                                                                       
Sbjct: 181 TGDDCISLGPGSKFINITNIQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFIGTSNGVR 240

BLAST of Csor.00g031080 vs. ExPASy TrEMBL
Match: A0A5D3B9J1 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001860 PE=3 SV=1)

HSP 1 Score: 165 bits (418), Expect = 4.25e-46
Identity = 119/359 (33.15%), Postives = 145/359 (40.39%), Query Frame = 0

Query: 29  IQFGATADGKTDSTEALSRAWKEACGHNGHGIVLVPA-GSFLVLPLVLEGPCKGSIGLLI 88
           +QFGA  D  TD+++ALS AW EAC +NG G+VL+PA G FLVLPL+L+GPC G I + +
Sbjct: 1   MQFGAKPDAITDNSKALSTAWSEACTNNGGGVVLIPALGRFLVLPLLLQGPCHGFIHIQL 60

Query: 89  NGKLLAPVSKNFAIGKYWLSISKVDNFFVNGSGSLNGGGSSAWHSDTSNRPIS------- 148
           +G+LLAP+++N   G YWLSI KV+N F++G G LNG GS+AW S + NRPIS       
Sbjct: 61  DGELLAPLNEN--TGDYWLSIDKVNNLFIDGLGRLNGRGSTAWPSASHNRPISMKLTGIN 120

Query: 149 ------------------------------------------------------------ 208
                                                                       
Sbjct: 121 NARISNIKSYNSKLFHFAVHGCRDVTFDHVTVIAPANSPNTDGIHVSLSSGINIMHSTIG 180

Query: 209 ------------------------------------------------------------ 213
                                                                       
Sbjct: 181 TGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFLGTTNGAR 240

BLAST of Csor.00g031080 vs. TAIR 10
Match: AT4G35670.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 91.3 bits (225), Expect = 1.0e-18
Identity = 55/169 (32.54%), Postives = 94/169 (55.62%), Query Frame = 0

Query: 24  KKFNIIQFGATADGKTDSTEALSRAWKEACGHNGH-GIVLVPAG-SFLVLPLVLEGPCK- 83
           K +N++ F A  DG+TD +EA  +AW  ACG +G    +L+P+G +FL+ P V +GPCK 
Sbjct: 22  KNYNVLNFDAKGDGQTDDSEAFLQAWTAACGGDGDIKTLLIPSGKTFLLQPTVFQGPCKS 81

Query: 84  GSIGLLINGKLLAPVSK---NFAIGKYWLSISKVDNFFVNGSGSLNGGGSSAWHSD--TS 143
            SI + ++G ++AP  K   +  I + W+  S V    + GSG+++  GSS W  +   S
Sbjct: 82  SSIKVQLDGTIVAPSDKFAWSDPISRMWIKFSTVSGLIIVGSGTIDSRGSSFWELNLKAS 141

Query: 144 NRPISNSKVQIKDVRYEGIRGSSTSQAAVSFECSQVCSCESIVLQDINL 185
            RP +    +  ++R  GI    + +  +S +     +C ++ + +INL
Sbjct: 142 QRPTALHISKCDNLRINGITSIDSPKNHISIK-----TCNTVAISNINL 185

BLAST of Csor.00g031080 vs. TAIR 10
Match: AT1G05650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 8.7e-18
Identity = 59/192 (30.73%), Postives = 92/192 (47.92%), Query Frame = 0

Query: 8   VFFIILLSIPKCYSQSKKFNIIQFGATADGKTDSTEALSRAWKEACGHNGHGIVLVPAGS 67
           + F +L  I    S S  FN++ FGA  DG TDST A  +AW+ ACG      V+VP G+
Sbjct: 10  LIFTLLTFIDVSSSASIVFNVVSFGAKPDGVTDSTAAFLKAWQGACGSAASATVVVPTGT 69

Query: 68  FLVLPLVLEGPCKGSIGLLINGKLLAPVS-KNFAIGKYWLSISKVDNFFVNGSGSLNGGG 127
           FL+  +   GPCK  I   + G ++AP   + F     W+  +KV+ F + G G+ +  G
Sbjct: 70  FLLKVITFGGPCKSKITFQVTGTVVAPEDYRTFGNSGSWILFNKVNRFSLVG-GTFDARG 129

Query: 128 SSAWHSDTSNRPI-----SNSKVQIKDVRYEGIRGSSTSQAAVSFECSQVCSCESIVLQD 187
           S  W    S +       S S    KDV   G++  ++  + ++        C ++ +++
Sbjct: 130 SGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVSHMTLN-----GCTNVAVRN 189

Query: 188 INLSYKGDGEPT 194
           I L   GD   T
Sbjct: 190 IRLVAPGDSPNT 195

BLAST of Csor.00g031080 vs. TAIR 10
Match: AT1G78400.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 8.7e-18
Identity = 44/107 (41.12%), Postives = 60/107 (56.07%), Query Frame = 0

Query: 24  KKFNIIQFGATADGKTDSTEALSRAWKEACGHNGHGIVLVPAGSFLVLPLVLEGPCKGSI 83
           K F++  +GA  DGKTD+T A ++AWK+AC   G   V +P G+F +  +   GPCK  I
Sbjct: 33  KVFDVRSYGARGDGKTDNTMAFTKAWKDACQWKGLPRVYIPFGTFYLGAVAFTGPCKSRI 92

Query: 84  GLLINGKLLAPVSKNFAIGKYWLSISKVDNFFVNGSGSLNGGGSSAW 131
             +I G LLAP   N      W+    VD   V+G G L+G GS +W
Sbjct: 93  SFIIKGTLLAPKDPNAIKQDSWIIFRYVDYLTVSGGGILDGQGSYSW 139


HSP 2 Score: 67.0 bits (162), Expect = 2.1e-11
Identity = 33/75 (44.00%), Postives = 52/75 (69.33%), Query Frame = 0

Query: 140 SNSKVQIKDVRYEGIRGSSTSQAAVSFECSQVCSCESIVLQDINLSYKG-DGEPTSICHN 199
           S SK+QI+DV+++ I G+ST++ AV  +CS+   C+++ L +IN+ ++G DG  TS+C N
Sbjct: 329 SFSKIQIRDVKFQNIWGTSTAKEAVKLQCSKNVPCKNVQLFNINIVHRGRDGPATSVCEN 388

Query: 200 VKGSAFGKQLPSSCL 214
           V G   GK  P SC+
Sbjct: 389 VGGWIGGKISPPSCI 403

BLAST of Csor.00g031080 vs. TAIR 10
Match: AT1G17150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 87.0 bits (214), Expect = 1.9e-17
Identity = 41/124 (33.06%), Postives = 71/124 (57.26%), Query Frame = 0

Query: 7   LVFFIILLSIPKCYSQSKKFNIIQFGATADGKTDSTEALSRAWKEACGHNGHGIVLVPAG 66
           L+  +I ++  +  ++ K F++  +GA  DGK D+T A + AWK+AC  NG   + +P G
Sbjct: 14  LLLLLIAVTTSRPITRPKVFDVRSYGAKGDGKMDNTNAFTNAWKDACRWNGPSKMYIPLG 73

Query: 67  SFLVLPLVLEGPCKGSIGLLINGKLLAPVSKNFAIGKYWLSISKVDNFFVNGSGSLNGGG 126
           +F +  +   GPC G I  +I+G LLAP + +    + W++   ++   V G G+L+G G
Sbjct: 74  TFYLGGVTFVGPCDGKISFVIDGTLLAPPNNDDIKKEIWINFRYINYLTVFGDGTLDGQG 133

Query: 127 SSAW 131
             +W
Sbjct: 134 KKSW 137


HSP 2 Score: 67.4 bits (163), Expect = 1.6e-11
Identity = 32/76 (42.11%), Postives = 51/76 (67.11%), Query Frame = 0

Query: 139 ISNSKVQIKDVRYEGIRGSSTSQAAVSFECSQVCSCESIVLQDINLSYKG-DGEPTSICH 198
           + +S +QI++V  + I G+S ++ AV F+CS+   C+ + L DINL++ G DG  +++C 
Sbjct: 326 MGDSHIQIRNVTLKNIWGTSKNKVAVKFQCSKTFPCKDVQLIDINLTHHGVDGPASALCE 385

Query: 199 NVKGSAFGKQLPSSCL 214
           NV GSA GK +P  CL
Sbjct: 386 NVDGSATGKMVPPHCL 401

BLAST of Csor.00g031080 vs. TAIR 10
Match: AT2G33160.1 (glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein )

HSP 1 Score: 85.5 bits (210), Expect = 5.6e-17
Identity = 47/131 (35.88%), Postives = 69/131 (52.67%), Query Frame = 0

Query: 8   VFFIILLSIPKCYSQSKKFNIIQFGATADGKTDSTEALSRAWKEACGHN-GHGIVLVPAG 67
           +FF +  S  +   Q K F++  +GA AD + D+  A ++AW EAC  + G   V +P+G
Sbjct: 14  LFFFLAASTKEYPGQPKIFDVRNYGARADSQRDNAFAFTKAWNEACQWSYGRSTVYIPSG 73

Query: 68  SFLVLPLVLEGPCKGSIGLLINGKLLAPVSKNFAIGKYWLSISKVDNFFVNGSGSLNGGG 127
            F +  +   GPCK SI   I G LLAP +      + W+    VDN  V G G L+G G
Sbjct: 74  IFYLRQVTFSGPCKSSITFFIRGTLLAPRNPYAINQEEWILFKYVDNLTVTGGGLLDGQG 133

Query: 128 SSAWHSDTSNR 138
           S +W  +  N+
Sbjct: 134 SYSWPLNDCNK 144

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P353391.7e-2131.82Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1[more]
P353385.3e-2030.11Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1[more]
P262169.1e-2030.11Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1[more]
Q397861.9e-1730.26Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1[more]
Q397665.5e-1729.74Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6577492.14.30e-150100.00Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6577490.15.98e-12987.98Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6577493.18.94e-12886.18Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022932248.12.34e-9948.42exopolygalacturonase-like [Cucurbita moschata][more]
KAG6577489.11.05e-9164.98Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1F1531.13e-9948.42exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111438612 PE=3 SV=... [more]
A0A6J1L8324.79e-6638.05exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111500170 PE=3 SV=1[more]
A0A6J1L5E93.60e-4850.49exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111500171 PE=3 SV=1[more]
A0A0A0L7H91.16e-4633.06Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G063610 PE=3 SV=1[more]
A0A5D3B9J14.25e-4633.15Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
Match NameE-valueIdentityDescription
AT4G35670.11.0e-1832.54Pectin lyase-like superfamily protein [more]
AT1G05650.18.7e-1830.73Pectin lyase-like superfamily protein [more]
AT1G78400.18.7e-1841.12Pectin lyase-like superfamily protein [more]
AT1G17150.11.9e-1733.06Pectin lyase-like superfamily protein [more]
AT2G33160.15.6e-1735.88glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family pro... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 137..201
e-value: 1.9E-9
score: 37.0
coord: 53..135
e-value: 9.8E-9
score: 34.7
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 136..211
e-value: 4.3E-13
score: 50.9
coord: 18..135
e-value: 6.2E-23
score: 83.4
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 140..211
coord: 4..135
NoneNo IPR availablePANTHERPTHR31375:SF147EXOPOLYGALACTURONASE-LIKEcoord: 140..211
NoneNo IPR availablePANTHERPTHR31375:SF147EXOPOLYGALACTURONASE-LIKEcoord: 4..135
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 10..139
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 140..213

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g031080.m01Csor.00g031080.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity