Homology
BLAST of Csor.00g030640 vs. ExPASy Swiss-Prot
Match:
F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)
HSP 1 Score: 859.4 bits (2219), Expect = 3.2e-248
Identity = 442/766 (57.70%), Postives = 539/766 (70.37%), Query Frame = 0
Query: 5 NLPPLLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLGSVLG 64
+L L L L + A KKSYIVYLGSH+H S+ L +H L S +G
Sbjct: 17 SLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVG 76
Query: 65 SNEAAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWNFLGV 124
S+E AK+AIFYSY RHINGFAA+LD+ A +IAKHPDV+SV NKG KLHTT SWNF+ +
Sbjct: 77 SHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLL 136
Query: 125 ENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGGFNFS 184
+G V +SLWN + +GE TII N+DTGVWPESKSFSDEGYG +P RWKG C +
Sbjct: 137 AKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCHK--DVP 196
Query: 185 CNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFFGNGN 244
CNRKLIGARYFNKGY++ P N+SYET RDH+GHG+HTLSTA GNFV G + FG GN
Sbjct: 197 CNRKLIGARYFNKGYLAYT-GLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGN 256
Query: 245 GTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTEEFSD 304
GTA GGSPKA VAAY+VCWP + CF ADILA IEAAI DGVDVLS S+GG+ ++
Sbjct: 257 GTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMS 316
Query: 305 NLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTLGDKR 364
+ IAIGSFHAVKNG+TVVCSAGNSGP G+V NVAPW+ITVGAS++DR F V L + +
Sbjct: 317 DGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQ 376
Query: 365 HFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVCLNWD 424
FKG SL SK +P EK Y +I A DA N + DA LC KGSLDP+K KGKI+VCL D
Sbjct: 377 SFKGTSL-SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGD 436
Query: 425 DIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRSNKNP 484
+ R++KG A AGA GM+L N+K SG +I+DAH+LPAS I Y DGE + Y+ S K+P
Sbjct: 437 NARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDP 496
Query: 485 IAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSITGLP 544
YI L TKPAP MASFSSRGPN + P ILKPDITAPG+NI+AAF+E T L
Sbjct: 497 KGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLD 556
Query: 545 YDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLNPILS 604
D RR FN SGTSM+CPHI+G+VGLLKTL+P WSPAAI+S IMTT+ T N P++
Sbjct: 557 SDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVD 616
Query: 605 SEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDTSFVR 664
++ANP +YG+GHVQPNKAA PGLVYDL+T DYL+FLCA GY+ ++LF+ D +
Sbjct: 617 ESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTC 676
Query: 665 SKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPSTLKF 724
+ ++D NYPSI++ NLT + + R++KNVG TY A+ P GV VSV+P L F
Sbjct: 677 RQGANLLDFNYPSITVPNLTGS-ITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTF 736
Query: 725 TNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTL 771
T E K F++ LR +P + VFG+L W+D H V SPI V L
Sbjct: 737 NKTGEVKIFQMTLRPLPVTPS-GYVFGELTWTDSHHYVRSPIVVQL 776
BLAST of Csor.00g030640 vs. ExPASy Swiss-Prot
Match:
Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)
HSP 1 Score: 843.6 bits (2178), Expect = 1.8e-243
Identity = 425/765 (55.56%), Postives = 541/765 (70.72%), Query Frame = 0
Query: 10 LLSFFLCALLQTSTIAAK--KSYIVYLGSHSHGLNPSALDLQLATQTHYNLLGSVLGSNE 69
LL L + +A+K SY+VY G+HSH + + +THY+ LGS GS E
Sbjct: 10 LLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRE 69
Query: 70 AAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWNFLGVEND 129
A AIFYSY +HINGFAA LD +A +I+KHP+V+SV NK LKLHTTRSW+FLG+E++
Sbjct: 70 RATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHN 129
Query: 130 GGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEG--GFNFSC 189
VPS+S+W + FGE TII N+DTGVWPESKSF DEG GPIP+RWKG C+ F C
Sbjct: 130 SYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHC 189
Query: 190 NRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFFGNGNG 249
NRKLIGARYFNKGY + V LNSS+++ RD +GHG+HTLSTA G+FV GVS FG GNG
Sbjct: 190 NRKLIGARYFNKGYAAAV--GHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNG 249
Query: 250 TAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTEEFSDN 309
TAKGGSP+A VAAY+VCWP + C+ AD+LA +AAI DG DV+S+SLGG F ++
Sbjct: 250 TAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFND 309
Query: 310 LIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTLGDKRH 369
+AIGSFHA K I VVCSAGNSGP + +V NVAPW ITVGAST+DR F +N+ LG+ +H
Sbjct: 310 SVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKH 369
Query: 370 FKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVCLNWDD 429
+KG SLSS +P KFYPI+ +++AK N+ +DA LC GSLDP K KGKI+VCL +
Sbjct: 370 YKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQN 429
Query: 430 IRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRSNKNPI 489
R+EKG G +GM+L N +G L+AD H+LPA+ ++ D V +YI K PI
Sbjct: 430 GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPI 489
Query: 490 AYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSITGLPY 549
A+ITP +T+LG KPAP+MASFSS+GP+ V P ILKPDITAPG++++AA++ S T +
Sbjct: 490 AHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQF 549
Query: 550 DKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLNPILSS 609
D RR+LFN +SGTSM+CPHI+GI GLLKT YP WSPAAI+S IMTTA + PI ++
Sbjct: 550 DPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNA 609
Query: 610 EKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDTSFVRS 669
+A P ++GAGHVQPN A +PGLVYDL +DYLNFLC+ GY+ Q+ +FS + S
Sbjct: 610 TNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSS 669
Query: 670 KSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPSTLKFT 729
+++LNYPSI++ NLTS V + R VKNVG Y +V +P GV V+V P++L FT
Sbjct: 670 PKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFT 729
Query: 730 NTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTL 771
E+K FKV+L + N K VFG+LVWSD KHRV SPI V L
Sbjct: 730 KVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772
BLAST of Csor.00g030640 vs. ExPASy Swiss-Prot
Match:
I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)
HSP 1 Score: 711.8 bits (1836), Expect = 8.2e-204
Identity = 395/788 (50.13%), Postives = 511/788 (64.85%), Query Frame = 0
Query: 4 FNLPPLLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLGSVL 63
F L +L SFFL L + +KK YIVY+G+HSHG +P++ DL+LAT +HY+LLGS+
Sbjct: 6 FCLHLILSSFFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIF 65
Query: 64 GSNEAAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWNFLG 123
GS E AK+AI YSYNRHINGFAA+L+++ A DIAK+P+V+SV +K KLHTTRSW FLG
Sbjct: 66 GSREKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLG 125
Query: 124 VENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGS-CE---- 183
+ G NS W FGE+TIIGNIDTGVWPES+SFSD+GYG +P++W+G C+
Sbjct: 126 LHRRG---QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKL 185
Query: 184 -GGFNFSCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGV 243
G +CNRKLIGARY+NK + L+ T RD GHGTHTLSTAGGNFV G
Sbjct: 186 PGSMKNTCNRKLIGARYYNKAF--EAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGA 245
Query: 244 SFFGNGNGTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGG 303
F GNGTAKGGSP+A VAAY+VCW C+ AD+LA I+ AI DGVDV+++S G
Sbjct: 246 RVFAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGV 305
Query: 304 N----TEEFSDNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRL 363
+ E + I+IG+FHA+ I +V SAGN GP G+V NVAPW+ T+ AST+DR
Sbjct: 306 SYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRD 365
Query: 364 FTTNVTLGDKRHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKA 423
F++N+T+ ++ +G SL +P + + +I + DAK N+ DA LC +G+LD K
Sbjct: 366 FSSNLTINNQL-IEGASL-FVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKV 425
Query: 424 KGKIVVCLNWDDIR-LEKGYVADEAGAVGMILVNNKGSGEGLIADAHLL-----PASHIS 483
GKIV+C I+ + +G A AGA GMIL N +G+ L A+ H+ P
Sbjct: 426 NGKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAK 485
Query: 484 YTDGELVDQYIRSNKNPI-----AYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPD 543
+ I +P+ ++ +T G KPAP+MASFSSRGPN ++PSILKPD
Sbjct: 486 SRPHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPD 545
Query: 544 ITAPGINILAAFSEENSITGLPYDKRR-VLFNVLSGTSMACPHIAGIVGLLKTLYPKWSP 603
+TAPG+NILAA+SE S + L D RR FNVL GTSM+CPH +GI GLLKT +P WSP
Sbjct: 546 VTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSP 605
Query: 604 AAIKSVIMTTAETEANDLNPILSS-EKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYL 663
AAIKS IMTTA T N PI + +K A+ AYG+GHV+P+ A +PGLVYDLS DYL
Sbjct: 606 AAIKSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYL 665
Query: 664 NFLCAHGYDKEQMKLFSNDTSFVRSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSS 723
NFLCA GYD++ + + + +F+ S S V DLNYPSI++ NL V I R V NVG
Sbjct: 666 NFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPP 725
Query: 724 GTYVAQVESPPGVSVSVDPSTLKFTNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKH 769
TY SP G S++V P +L FT E K FKV+++ ++ FG L W+DGKH
Sbjct: 726 STYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKH 785
BLAST of Csor.00g030640 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 617.5 bits (1591), Expect = 2.1e-175
Identity = 342/767 (44.59%), Postives = 480/767 (62.58%), Query Frame = 0
Query: 9 LLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLGSVLGSNEA 68
LLL C + +S+ + + +YIV++ PS+ DL H N S L S
Sbjct: 13 LLLCLGFCHV--SSSSSDQGTYIVHMAKSQ---MPSSFDL------HSNWYDSSLRSISD 72
Query: 69 AKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWNFLGVENDG 128
+ + + Y+Y I+GF+ L Q+ A+ + P VISV +LHTTR+ FLG++
Sbjct: 73 SAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH- 132
Query: 129 GVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGGFNFS---C 188
+ L+ + ++G +DTGVWPESKS+SDEG+GPIP+ WKG CE G NF+ C
Sbjct: 133 ---TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLC 192
Query: 189 NRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFFGNGNG 248
NRKLIGAR+F +GY S + P + + RD +GHGTHT STA G+ VEG S G +G
Sbjct: 193 NRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASG 252
Query: 249 TAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTEEFSDN 308
TA+G +P+A VA Y+VCW GGCF +DILA I+ AI+D V+VLS+SLGG ++ +
Sbjct: 253 TARGMAPRARVAVYKVCW----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRD 312
Query: 309 LIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTLGDKRH 368
+AIG+F A++ GI V CSAGN+GP S+ NVAPWI TVGA T+DR F LG+ ++
Sbjct: 313 GVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKN 372
Query: 369 FKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVCLNWDD 428
F G+SL +K P I A N S+ + LC+ G+L PEK KGKIV+C +
Sbjct: 373 FTGVSLFKGEALPDKLLPFIYA----GNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN 432
Query: 429 IRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRSNKNPI 488
R++KG V AG VGMIL N +GE L+ADAHLLPA+ + G+++ Y+ ++ NP
Sbjct: 433 ARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPT 492
Query: 489 AYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSITGLPY 548
A I+ + T +G KP+P++A+FSSRGPN + P+ILKPD+ APG+NILAA++ TGL
Sbjct: 493 ASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLAS 552
Query: 549 DKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLNPILS- 608
D RRV FN++SGTSM+CPH++G+ LLK+++P+WSPAAI+S +MTTA D P+L
Sbjct: 553 DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI 612
Query: 609 SEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFS-NDTSFV 668
+ + + P +GAGHV P A +PGL+YDL+T+DYL FLCA Y Q++ S + +
Sbjct: 613 ATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCD 672
Query: 669 RSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVES-PPGVSVSVDPSTL 728
SKS+ V DLNYPS ++ R V +VG +GTY +V S GV +SV+P+ L
Sbjct: 673 PSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVL 732
Query: 729 KFTNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVT 770
F +E+K + V + + N FG + WSDGKH V SP+ ++
Sbjct: 733 NFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755
BLAST of Csor.00g030640 vs. ExPASy Swiss-Prot
Match:
Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)
HSP 1 Score: 565.8 bits (1457), Expect = 7.3e-160
Identity = 328/767 (42.76%), Postives = 457/767 (59.58%), Query Frame = 0
Query: 10 LLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLGSVLGSNEAA 69
+++ FL LL T+ AKK+YI+ + +H P + TH++ S L S
Sbjct: 13 IITTFLFLLLHTT---AKKTYIIRV---NHSDKPESF------LTHHDWYTSQLNS---- 72
Query: 70 KKAIFYSYNRHINGFAAVLDQKVAED-IAKHPDVISVHENKGLKLHTTRSWNFLGVENDG 129
+ ++ Y+Y +GF+A LD A+ ++ ++ + E+ LHTTR+ FLG+ ++
Sbjct: 73 ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEF 132
Query: 130 GVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGGFNFS---C 189
GV +L S IIG +DTGVWPES+SF D IP++WKG CE G +F C
Sbjct: 133 GV-----HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLC 192
Query: 190 NRKLIGARYFNKGYISVVEPKPLNSSYETT--RDHNGHGTHTLSTAGGNFVEGVSFFGNG 249
N+KLIGAR F+KG+ + +S E+ RD +GHGTHT +TA G+ V SF G
Sbjct: 193 NKKLIGARSFSKGF-QMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYA 252
Query: 250 NGTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTEEFS 309
GTA+G + +A VA Y+VCW S GCF +DILA ++ AI DGVDVLS+SLGG + +
Sbjct: 253 AGTARGMATRARVATYKVCW----STGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYY 312
Query: 310 DNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTLGDK 369
+ IAIG+F A++ G+ V CSAGNSGP SV NVAPW++TVGA T+DR F LG+
Sbjct: 313 RDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNG 372
Query: 370 RHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVCLNW 429
+ G+SL S K L+ +N + + LCL GSLD +GKIVVC
Sbjct: 373 KRLTGVSLYSGVGMGTK------PLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRG 432
Query: 430 DDIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRSNKN 489
+ R+EKG V +AG +GMI+ N SGE L+AD+HLLPA + G+L+ +Y++S+
Sbjct: 433 VNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSK 492
Query: 490 PIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSITGL 549
P A + T L KP+P++A+FSSRGPN V P ILKPD+ PG+NILA +S+ TGL
Sbjct: 493 PTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGL 552
Query: 550 PYDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLNPIL 609
D RR FN++SGTSM+CPHI+G+ GLLK +P+WSP+AIKS +MTTA N P+
Sbjct: 553 DKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLH 612
Query: 610 -SSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDTSF 669
+++ +NP A+G+GHV P KA PGLVYD+ST++Y+ FLC+ Y + + S
Sbjct: 613 DAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSV 672
Query: 670 VRSKSFK-VIDLNYPSISIYNLTSDVVNIKRRVKNVG-SSGTYVAQVESPPGVSVSVDPS 729
SK F LNYPS S+ VV R V NVG +S Y V P V +SV PS
Sbjct: 673 NCSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPS 732
Query: 730 TLKFTNTDEEKDFKVV-LRRVPNNQTKENVFGKLVWSDGKHRVSSPI 767
L F + E+K + V + + + T + FG + WS+ +H V SP+
Sbjct: 733 KLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPV 747
BLAST of Csor.00g030640 vs. NCBI nr
Match:
KAG6577535.1 (Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1544 bits (3998), Expect = 0.0
Identity = 772/772 (100.00%), Postives = 772/772 (100.00%), Query Frame = 0
Query: 1 MVAFNLPPLLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLG 60
MVAFNLPPLLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLG
Sbjct: 1 MVAFNLPPLLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWN 120
SVLGSNEAAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWN
Sbjct: 61 SVLGSNEAAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWN 120
Query: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
Query: 181 FNFSCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFF 240
FNFSCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFF
Sbjct: 181 FNFSCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFF 240
Query: 241 GNGNGTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTE 300
GNGNGTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTE
Sbjct: 241 GNGNGTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTE 300
Query: 301 EFSDNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTL 360
EFSDNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTL
Sbjct: 301 EFSDNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTL 360
Query: 361 GDKRHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVC 420
GDKRHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVC
Sbjct: 361 GDKRHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVC 420
Query: 421 LNWDDIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRS 480
LNWDDIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRS
Sbjct: 421 LNWDDIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRS 480
Query: 481 NKNPIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSI 540
NKNPIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSI
Sbjct: 481 NKNPIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSI 540
Query: 541 TGLPYDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLN 600
TGLPYDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLN
Sbjct: 541 TGLPYDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLN 600
Query: 601 PILSSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDT 660
PILSSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDT
Sbjct: 601 PILSSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDT 660
Query: 661 SFVRSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPS 720
SFVRSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPS
Sbjct: 661 SFVRSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPS 720
Query: 721 TLKFTNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTLQM 772
TLKFTNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTLQM
Sbjct: 721 TLKFTNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTLQM 772
BLAST of Csor.00g030640 vs. NCBI nr
Match:
XP_023552945.1 (subtilisin-like protease SBT5.4 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1491 bits (3860), Expect = 0.0
Identity = 743/772 (96.24%), Postives = 756/772 (97.93%), Query Frame = 0
Query: 1 MVAFNLPPLLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLG 60
M AFNLPPLLLSFFL ALLQTSTIAA KSYIVYLGSHSHGLNPS LDLQLATQTHY+LLG
Sbjct: 1 MEAFNLPPLLLSFFLFALLQTSTIAANKSYIVYLGSHSHGLNPSVLDLQLATQTHYSLLG 60
Query: 61 SVLGSNEAAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWN 120
SVLGSNEAAK+AIFYSYNRHINGFAAVLDQKVA+DIAKH DVISVHENKGLKLHTTRSWN
Sbjct: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDQKVAKDIAKHRDVISVHENKGLKLHTTRSWN 120
Query: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSF DEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFRDEGYGPIPTRWKGSCEGG 180
Query: 181 FNFSCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFF 240
FNFSCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFF
Sbjct: 181 FNFSCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFF 240
Query: 241 GNGNGTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTE 300
GNGNGT KGGSPKALVAAYRVCWPS SG CFMADILAGIEAAISDGVDVLSISLGGNTE
Sbjct: 241 GNGNGTTKGGSPKALVAAYRVCWPSTRSGACFMADILAGIEAAISDGVDVLSISLGGNTE 300
Query: 301 EFSDNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTL 360
EFSDNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGAST+DRLFTTNVTL
Sbjct: 301 EFSDNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTMDRLFTTNVTL 360
Query: 361 GDKRHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVC 420
GDKRHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEK KGKIVVC
Sbjct: 361 GDKRHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKVKGKIVVC 420
Query: 421 LNWDDIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRS 480
LNWDDIRLEKGYVA EAGAVGMILVNNKGSGEGL+ADAHLLPASHISYTDGELVDQYIRS
Sbjct: 421 LNWDDIRLEKGYVAAEAGAVGMILVNNKGSGEGLMADAHLLPASHISYTDGELVDQYIRS 480
Query: 481 NKNPIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSI 540
NK+PIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPG+NILAAFSEENS+
Sbjct: 481 NKHPIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGVNILAAFSEENSV 540
Query: 541 TGLPYDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLN 600
TGLPYDKRRVLFNVLSGTSMACPHIAGIV LLKTLYPKWSPAAI+S IMTTAETEANDLN
Sbjct: 541 TGLPYDKRRVLFNVLSGTSMACPHIAGIVSLLKTLYPKWSPAAIRSAIMTTAETEANDLN 600
Query: 601 PILSSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDT 660
PILSSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDT
Sbjct: 601 PILSSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDT 660
Query: 661 SFVRSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPS 720
SFV SKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGS GTYVAQVE+PPGVSVS+DP+
Sbjct: 661 SFVCSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSPGTYVAQVEAPPGVSVSIDPN 720
Query: 721 TLKFTNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTLQM 772
TLKFTNTDEEKDFKVVLR+VPNNQTKENVFGKLVWSDGKHRVSSPIFVTLQM
Sbjct: 721 TLKFTNTDEEKDFKVVLRKVPNNQTKENVFGKLVWSDGKHRVSSPIFVTLQM 772
BLAST of Csor.00g030640 vs. NCBI nr
Match:
KAG6577554.1 (Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1271 bits (3290), Expect = 0.0
Identity = 639/772 (82.77%), Postives = 696/772 (90.16%), Query Frame = 0
Query: 1 MVAFNLPPLLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLG 60
M AFNLPPLLLSFFL +LLQTSTIAAKKSYIVYLGSHSHGLNPSA+DLQL TQTHYNLLG
Sbjct: 1 MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLG 60
Query: 61 SVLGSNEAAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWN 120
S+LGS EAAK+AIFYSYNRHIN FAAVLDQKV EDIAKHPDV+SV+ENK LKLHTTRSWN
Sbjct: 61 SMLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN 120
Query: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
FL VEN+GG+PS+SLW LS FGESTIIGN+D+GVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLRVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
Query: 181 FNFSCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFF 240
NF CNRKLIGARYFNKGY S V P LNSSYET RD +GHGTHTLSTAGGNFV+ VS F
Sbjct: 181 SNFRCNRKLIGARYFNKGYASNVGP--LNSSYETARDDDGHGTHTLSTAGGNFVQRVSMF 240
Query: 241 GNGNGTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTE 300
GNG GTAKGGSPKA VAAYRVCWP +G+GGCFMADILAG EAAISDGVDVLSISLGG
Sbjct: 241 GNGYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAI 300
Query: 301 EFSDNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTL 360
EFS++L+AI S+HAVKNGITVVCSAGNSGPF G+V NVAPW+ITVGAST+DRLFT++V L
Sbjct: 301 EFSEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVL 360
Query: 361 GDKRHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVC 420
GDKRHFKG SLSSK +PA+KFYP+IRALDAKFNN D DA LC +GSLDP+K KGKIVVC
Sbjct: 361 GDKRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVC 420
Query: 421 LNWDDIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRS 480
L D+ R+EKGYV +AGAVGMIL N+K +G+ ADAHLLP SHI YTDGE V QYI+S
Sbjct: 421 LRGDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQS 480
Query: 481 NKNPIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSI 540
K PIAY+T V TELG KPAPIMASFSSRGPN +EPSILKPDITAPG+NI+AAFSE SI
Sbjct: 481 TKTPIAYVTQV-TELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSI 540
Query: 541 TGLPYDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLN 600
TGLPYDKRRV F LSGTSM+CPHI+GIVGLLKTL+PKWSPAAI+S IMTTAETEANDLN
Sbjct: 541 TGLPYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLN 600
Query: 601 PILSSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDT 660
PIL+SEKE+ANPLAYGAGHVQPNKAA+PGLVYDLSTQDYLNFLCA GY+K QMKLF+NDT
Sbjct: 601 PILTSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDT 660
Query: 661 SFVRSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPS 720
SFV SKSFKVIDLNYPSISIYNL S+VVNIKRRVKNVGS GTYVA+VESPPGVSVSV PS
Sbjct: 661 SFVCSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPS 720
Query: 721 TLKFTNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTLQM 772
TLKFT T EEK+FKVVLRRV +NQT+E+VFGKLVWS GKHRVSSPIFVTL++
Sbjct: 721 TLKFTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI 769
BLAST of Csor.00g030640 vs. NCBI nr
Match:
KAG6577555.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1269 bits (3283), Expect = 0.0
Identity = 635/772 (82.25%), Postives = 694/772 (89.90%), Query Frame = 0
Query: 1 MVAFNLPPLLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLG 60
M AFNLPPLL+SFFL LLQTSTIAAKKSYIVYLGSHSHGLNPS +DLQLATQTHYNLLG
Sbjct: 1 MEAFNLPPLLVSFFLLVLLQTSTIAAKKSYIVYLGSHSHGLNPSTIDLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWN 120
SVLGSNEAAK+AIFYSYNRHIN FAA+LDQKVAEDIAK PDV SV+ENKGL+LHTTRSWN
Sbjct: 61 SVLGSNEAAKEAIFYSYNRHINAFAAILDQKVAEDIAKRPDVASVYENKGLELHTTRSWN 120
Query: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
FLGVENDGG+PSNSLWNLS FGESTIIGN+D+GVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPSNSLWNLSKFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
Query: 181 FNFSCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFF 240
NFSCNRKLIGARYFNKGY S LNSSYET RD +GHGTHTLSTAGGNFV+ VS F
Sbjct: 181 SNFSCNRKLIGARYFNKGYAS--NGGHLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMF 240
Query: 241 GNGNGTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTE 300
GNG GTAKGGSPKALVAAY+VCWP++ SGGCFMADILAG EAAISDGVDVLSISLGG
Sbjct: 241 GNGYGTAKGGSPKALVAAYKVCWPAVASGGCFMADILAGFEAAISDGVDVLSISLGGGAR 300
Query: 301 EFSDNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTL 360
EFS++++AI SFHAVKNGITVVCSAGN GP EG+V NVAPW+ITVGAST+DR FTTNV L
Sbjct: 301 EFSEDILAIMSFHAVKNGITVVCSAGNFGPLEGTVSNVAPWMITVGASTVDRHFTTNVVL 360
Query: 361 GDKRHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVC 420
GDKRHFKG SLSSK +PA+K YP+IRALDAK NN D DA LC +GSL+PEKAKGKIVVC
Sbjct: 361 GDKRHFKGKSLSSKLLPAKKLYPLIRALDAKSNNVSDNDAILCHQGSLNPEKAKGKIVVC 420
Query: 421 LNWDDIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRS 480
L D+ R+EKGYV +AG +GMIL N+KGSG+ L DAHLLPA+HISYTDGE + QYI+S
Sbjct: 421 LRGDNTRVEKGYVVAQAGGIGMILANDKGSGDALSLDAHLLPAAHISYTDGESIYQYIQS 480
Query: 481 NKNPIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSI 540
K+P+AY+T V TELG KPAPIMA FSSRGPN VEP ILKPDITAPG+NI+AAFSE SI
Sbjct: 481 TKSPMAYMTHV-TELGIKPAPIMAPFSSRGPNTVEPLILKPDITAPGVNIIAAFSEATSI 540
Query: 541 TGLPYDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLN 600
TGLPYDKRRV F LSGTSM+CPHI+GIVGLLKTLYPKWSPAAI+S IMTTA TEANDLN
Sbjct: 541 TGLPYDKRRVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAVTEANDLN 600
Query: 601 PILSSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDT 660
PILS E E+ANPLAYGAGHVQPNKAA+PGLVYDLSTQDYLNFLCA GY+K+QMKLFSNDT
Sbjct: 601 PILSLENEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKKQMKLFSNDT 660
Query: 661 SFVRSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPS 720
SFV SKSFKVIDLNYPSIS+YNL S+VV IKRRVKNVGS GTYVA+VE+PPGV VSVDPS
Sbjct: 661 SFVCSKSFKVIDLNYPSISMYNLKSEVVKIKRRVKNVGSPGTYVAKVEAPPGVLVSVDPS 720
Query: 721 TLKFTNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTLQM 772
TLKFT T EEKDF+VVLRR+ +NQT+++VFGKLVWSDGKHRVSSPIFVTL++
Sbjct: 721 TLKFTKTAEEKDFQVVLRRIQSNQTEKHVFGKLVWSDGKHRVSSPIFVTLEI 769
BLAST of Csor.00g030640 vs. NCBI nr
Match:
KAG6577557.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1267 bits (3279), Expect = 0.0
Identity = 627/772 (81.22%), Postives = 695/772 (90.03%), Query Frame = 0
Query: 1 MVAFNLPPLLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLG 60
M AFNLPPLLLSFFL ALLQTSTIAAKKSYIVYLGSHSHGLNPSA+DLQLATQTHYNLLG
Sbjct: 1 MEAFNLPPLLLSFFLFALLQTSTIAAKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWN 120
SVLGSNEAAK+AIFYSYNRHIN FAAVLDQKVAEDIAK PDV+SV+ENKGLKLHTTRSWN
Sbjct: 61 SVLGSNEAAKEAIFYSYNRHINAFAAVLDQKVAEDIAKRPDVVSVYENKGLKLHTTRSWN 120
Query: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
FLGVENDGG+PSNSLWNLS FGESTIIGN+DTGVWPESKSFSD+GYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPSNSLWNLSRFGESTIIGNLDTGVWPESKSFSDKGYGPIPTRWKGSCEGG 180
Query: 181 FNFSCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFF 240
NF+CNRKLIGARYFNKGY SV P LNSSYE+ RD NGHGTHTLSTAGGNFVEGVS F
Sbjct: 181 SNFNCNRKLIGARYFNKGYASVAGP--LNSSYESARDDNGHGTHTLSTAGGNFVEGVSIF 240
Query: 241 GNGNGTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTE 300
GNG GTAKGGSPK+LVAAY+VCWP + GGCF+ADILAG EAAISDGVDVLS+SLGG +
Sbjct: 241 GNGYGTAKGGSPKSLVAAYKVCWPPVEDGGCFVADILAGFEAAISDGVDVLSVSLGGRVQ 300
Query: 301 EFSDNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTL 360
EF D+++AIGSFHAVKNGITVVCSAGNSGP+EG+ NVAPW++TVGASTIDRLFTTNVTL
Sbjct: 301 EFYDDILAIGSFHAVKNGITVVCSAGNSGPYEGTASNVAPWMLTVGASTIDRLFTTNVTL 360
Query: 361 GDKRHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVC 420
GDKRHFKG SLS K +PA+KFYP+IRALDAKFNN D +A LCL+G+LDP+K +GKIVVC
Sbjct: 361 GDKRHFKGESLSGKILPAKKFYPLIRALDAKFNNVSDYEAGLCLEGTLDPKKVEGKIVVC 420
Query: 421 LNWDDIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRS 480
L D+ R++KGYV +AG VGMIL N+ G L+++AH+LPASHISYTDGE V +YI+S
Sbjct: 421 LRGDNSRVDKGYVVAQAGGVGMILANDLLGGRALLSEAHILPASHISYTDGESVYKYIQS 480
Query: 481 NKNPIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSI 540
K P+AY+T V+TELG KPAPIMASFSSRGPN +EP+ILKPDITAPG+NILAAFS
Sbjct: 481 TKTPMAYMTSVRTELGIKPAPIMASFSSRGPNVIEPTILKPDITAPGVNILAAFSNIAPP 540
Query: 541 TGLPYDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLN 600
T +DKRRVL+NVLSGTSM+CPHI+GIVGLLKTLYPKWSPAAI+S IMTTAET+ANDLN
Sbjct: 541 THSHFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETKANDLN 600
Query: 601 PILSSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDT 660
PIL+S +E+A P AYGAGHVQPNKAA+PGLVYDLSTQDYLNFLCA GY+K QM+LF+NDT
Sbjct: 601 PILTSIEEKAGPFAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKTQMRLFTNDT 660
Query: 661 SFVRSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPS 720
SFV SKSFKV DLNYPSISI++L S VNIKRRVKNVGS GTYVA+VE+PPG+ VSVDPS
Sbjct: 661 SFVCSKSFKVTDLNYPSISIFDLRSKEVNIKRRVKNVGSPGTYVAKVEAPPGILVSVDPS 720
Query: 721 TLKFTNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTLQM 772
TLKFT DEEKDFKVVL+RVPNNQT+ +VFGKLVWSDGKHRVSSPI VTL M
Sbjct: 721 TLKFTKPDEEKDFKVVLKRVPNNQTENHVFGKLVWSDGKHRVSSPISVTLLM 770
BLAST of Csor.00g030640 vs. ExPASy TrEMBL
Match:
A0A6J1EWJ2 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111438754 PE=3 SV=1)
HSP 1 Score: 1267 bits (3278), Expect = 0.0
Identity = 637/772 (82.51%), Postives = 694/772 (89.90%), Query Frame = 0
Query: 1 MVAFNLPPLLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLG 60
M AFNLPPLLLSFFL +LLQTSTIAA KSYIVYLGSHSHGLNPS +DLQL TQTHYNLLG
Sbjct: 1 MEAFNLPPLLLSFFLFSLLQTSTIAANKSYIVYLGSHSHGLNPSTVDLQLTTQTHYNLLG 60
Query: 61 SVLGSNEAAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWN 120
SVLGS EAAK+AIFYSYNRHIN FAAVLDQKV EDIAK+PDV+SV+ENK LKLHTTRSWN
Sbjct: 61 SVLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKYPDVVSVYENKELKLHTTRSWN 120
Query: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
FLGVEN+GG+PS+SLW LS FGESTIIGN+D+GVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
Query: 181 FNFSCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFF 240
NF CNRKLIGARYFNKGY S V P LNSSYET RD +GHGTHTLSTAGGNFV+GVS F
Sbjct: 181 SNFRCNRKLIGARYFNKGYASNVGP--LNSSYETARDDDGHGTHTLSTAGGNFVQGVSMF 240
Query: 241 GNGNGTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTE 300
GNG GTAKGGSPKA VAAYRVCWP +G+GGCFMADILAG EAAISDGVDVLSISLGG
Sbjct: 241 GNGYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAI 300
Query: 301 EFSDNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTL 360
EFS++L+AI S+HAVKNGITVVCSAGNSGPF G+V NVAPW+ITVGAST+DRLFT++V L
Sbjct: 301 EFSEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVL 360
Query: 361 GDKRHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVC 420
GDKRHFKG SLSSK + A+KFYP+IRALDAKFNN D DA LC +GSLDP+K KGKIVVC
Sbjct: 361 GDKRHFKGKSLSSKLLLAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVC 420
Query: 421 LNWDDIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRS 480
L D+ R+EKGYV +AGAVGMIL N+K +G+ ADAHLLP SHISYTDGE V QYIRS
Sbjct: 421 LRGDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHISYTDGESVYQYIRS 480
Query: 481 NKNPIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSI 540
K PIAY+T V TELG KPAPIMASFSSRGPN +EPSILKPDITAPG+NI+AAFSE SI
Sbjct: 481 TKTPIAYVTQV-TELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSI 540
Query: 541 TGLPYDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLN 600
TGLPYDKRRV F LSGTSM+CPHI+GIVGLLKTL+PKWSPAAI+S IMTTA TEANDLN
Sbjct: 541 TGLPYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAGTEANDLN 600
Query: 601 PILSSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDT 660
PIL+SEKE+ANPLAYGAGHVQPNKAA+PGLVYDLSTQDYLNFLCA GY+K QMKLF+NDT
Sbjct: 601 PILTSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDT 660
Query: 661 SFVRSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPS 720
SFV SKSFKVIDLNYPSISIYNL S+VVNIKRRVKNVGS GTYVA+VESP GVS+SV PS
Sbjct: 661 SFVCSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPSGVSISVAPS 720
Query: 721 TLKFTNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTLQM 772
TLKFT T EEK+FKVVLRRV +NQT+E+VFGKLVWS GKHRVSSPIFVTL++
Sbjct: 721 TLKFTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI 769
BLAST of Csor.00g030640 vs. ExPASy TrEMBL
Match:
A0A6J1EW82 (subtilisin-like protease SBT5.3 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111438753 PE=3 SV=1)
HSP 1 Score: 1266 bits (3277), Expect = 0.0
Identity = 626/772 (81.09%), Postives = 695/772 (90.03%), Query Frame = 0
Query: 1 MVAFNLPPLLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLG 60
M AFNLPPLLLSFFL ALLQTSTIAAKKSYIVYLGSHSHGLNPSA+DLQLATQTHYNLLG
Sbjct: 1 MEAFNLPPLLLSFFLFALLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWN 120
SVLGSNEAAK+AIFYSYNRHINGFAAVLDQKVAED+AK PDV+SV+ENKGLKLHTTRSWN
Sbjct: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDQKVAEDVAKRPDVVSVYENKGLKLHTTRSWN 120
Query: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
FLGVENDGG+PSNSLWNLS FGESTIIGN+DTGVWPESKSFSD+ YGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPSNSLWNLSRFGESTIIGNLDTGVWPESKSFSDKEYGPIPTRWKGSCEGG 180
Query: 181 FNFSCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFF 240
NF+CNRKLIGARYFNKGY SV P LNSSYET RD NGHGTHTLSTAGGNFVEGVS F
Sbjct: 181 SNFNCNRKLIGARYFNKGYASVAGP--LNSSYETARDDNGHGTHTLSTAGGNFVEGVSIF 240
Query: 241 GNGNGTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTE 300
GNG GTAKGGSPK+LVAAY+VCWP + GGCF+ADILAG EAAISDGVDVLS+SLGG +
Sbjct: 241 GNGYGTAKGGSPKSLVAAYKVCWPPVEDGGCFVADILAGFEAAISDGVDVLSVSLGGRVQ 300
Query: 301 EFSDNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTL 360
EF D+++AIGSFHAVKNGITVVCSAGNSGP+EG+ NVAPW++TVGASTIDRLFTTNVTL
Sbjct: 301 EFYDDILAIGSFHAVKNGITVVCSAGNSGPYEGTASNVAPWMLTVGASTIDRLFTTNVTL 360
Query: 361 GDKRHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVC 420
GDKRHFKG SLS K +PA+KFYP+IRALDAKFNN D + LCL+G+LDP+K +GKIVVC
Sbjct: 361 GDKRHFKGESLSGKILPAKKFYPLIRALDAKFNNVSDYETGLCLEGTLDPKKVEGKIVVC 420
Query: 421 LNWDDIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRS 480
L D+ R++KGYV +AG VGMIL N+ G L+++AH+LPASHISYTDGE V +YI+S
Sbjct: 421 LRGDNSRVDKGYVVAQAGGVGMILANDLLGGRALLSEAHILPASHISYTDGESVYKYIQS 480
Query: 481 NKNPIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSI 540
K P+AY+T V+TELG KPAPIMASFSSRGPN +EP+ILKPDITAPG+NILAAFS
Sbjct: 481 TKTPMAYMTSVRTELGIKPAPIMASFSSRGPNVIEPTILKPDITAPGVNILAAFSNIAPP 540
Query: 541 TGLPYDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLN 600
T +DKRRVL+NVLSGTSM+CPHI+GIVGLLKTLYPKWSPAAI+S IMTTAET+ANDLN
Sbjct: 541 THSHFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETKANDLN 600
Query: 601 PILSSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDT 660
PIL+S +E+A P AYGAGHVQPNKAA+PGLVYDLSTQDYLNFLCA GY+K QM+LF+NDT
Sbjct: 601 PILTSIEEKAGPFAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKTQMRLFTNDT 660
Query: 661 SFVRSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPS 720
SFV SKSFKV DLNYPSISI++L S VNIKRRVKNVGS GTYVA+VE+PPG+ VSVDPS
Sbjct: 661 SFVCSKSFKVTDLNYPSISIFDLRSKEVNIKRRVKNVGSPGTYVAKVEAPPGILVSVDPS 720
Query: 721 TLKFTNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTLQM 772
TLKFT +DEEKDFKVVL+RVPNNQT+ +VFGKLVWSDGKHRVSSPI VTL M
Sbjct: 721 TLKFTKSDEEKDFKVVLKRVPNNQTENHVFGKLVWSDGKHRVSSPISVTLLM 770
BLAST of Csor.00g030640 vs. ExPASy TrEMBL
Match:
A0A6J1EWV1 (subtilisin-like protease SBT5.3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111438753 PE=3 SV=1)
HSP 1 Score: 1263 bits (3267), Expect = 0.0
Identity = 624/772 (80.83%), Postives = 693/772 (89.77%), Query Frame = 0
Query: 1 MVAFNLPPLLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLG 60
M AFNLPPLL+SFFL LLQTSTIAAKKSYIVYLGSHSHGLNPSA+DLQLATQTHYNLLG
Sbjct: 1 MEAFNLPPLLVSFFLLVLLQTSTIAAKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWN 120
SVLGSNEAAK+AIFYSYNRHIN FAAVLDQKVAEDIAK PDV+SV+ENKGLKLHTTRSWN
Sbjct: 61 SVLGSNEAAKEAIFYSYNRHINAFAAVLDQKVAEDIAKRPDVVSVYENKGLKLHTTRSWN 120
Query: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
FLGVENDGG+PSNSLWNLS FGESTIIGN+DTGVWPESKSFSD+ YGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPSNSLWNLSRFGESTIIGNLDTGVWPESKSFSDKEYGPIPTRWKGSCEGG 180
Query: 181 FNFSCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFF 240
NF+CNRKLIGARYFNKGY SV P LNSSYET RD NGHGTHTLSTAGGNFVEGVS F
Sbjct: 181 SNFNCNRKLIGARYFNKGYASVAGP--LNSSYETARDDNGHGTHTLSTAGGNFVEGVSIF 240
Query: 241 GNGNGTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTE 300
GNG GTAKGGSPK+LVAAY+VCWP + GGCF+ADILAG EAAISDGVDVLS+SLGG +
Sbjct: 241 GNGYGTAKGGSPKSLVAAYKVCWPPVEDGGCFVADILAGFEAAISDGVDVLSVSLGGRVQ 300
Query: 301 EFSDNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTL 360
EF D+++AIGSFHAVKNGITVVCSAGNSGP+EG+ NVAPW++TVGASTIDRLFTTNVTL
Sbjct: 301 EFYDDILAIGSFHAVKNGITVVCSAGNSGPYEGTASNVAPWMLTVGASTIDRLFTTNVTL 360
Query: 361 GDKRHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVC 420
GDKRHFKG SLS K +PA+KFYP+IRALDAKFNN D + LCL+G+LDP+K +GKIVVC
Sbjct: 361 GDKRHFKGESLSGKILPAKKFYPLIRALDAKFNNVSDYETGLCLEGTLDPKKVEGKIVVC 420
Query: 421 LNWDDIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRS 480
L D+ R++KGYV +AG VGMIL N+ G L+++AH+LPASHISYTDGE V +YI+S
Sbjct: 421 LRGDNSRVDKGYVVAQAGGVGMILANDLLGGRALLSEAHILPASHISYTDGESVYKYIQS 480
Query: 481 NKNPIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSI 540
K P+AY+T V+TELG KPAPIMASFSSRGPN +EP+ILKPDITAPG+NILAAFS
Sbjct: 481 TKTPMAYMTSVRTELGIKPAPIMASFSSRGPNVIEPTILKPDITAPGVNILAAFSNIAPP 540
Query: 541 TGLPYDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLN 600
T +DKRRVL+NVLSGTSM+CPHI+GIVGLLKTLYPKWSPAAI+S IMTTAET+ANDLN
Sbjct: 541 THSHFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETKANDLN 600
Query: 601 PILSSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDT 660
PIL+S +E+A P AYGAGHVQPNKAA+PGLVYDLSTQDYLNFLCA GY+K QM+LF+NDT
Sbjct: 601 PILTSIEEKAGPFAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKTQMRLFTNDT 660
Query: 661 SFVRSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPS 720
SFV SKSFKV DLNYPSISI++L S VNIKRRVKNVGS GTYVA+VE+PPG+ VSVDPS
Sbjct: 661 SFVCSKSFKVTDLNYPSISIFDLRSKEVNIKRRVKNVGSPGTYVAKVEAPPGILVSVDPS 720
Query: 721 TLKFTNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTLQM 772
TLKFT +DEEKDFKVVL+RVPNNQT+ +VFGKLVWSDGKHRVSSPI VTL M
Sbjct: 721 TLKFTKSDEEKDFKVVLKRVPNNQTENHVFGKLVWSDGKHRVSSPISVTLLM 770
BLAST of Csor.00g030640 vs. ExPASy TrEMBL
Match:
A0A6J1F1I2 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111438753 PE=3 SV=1)
HSP 1 Score: 1262 bits (3266), Expect = 0.0
Identity = 624/772 (80.83%), Postives = 693/772 (89.77%), Query Frame = 0
Query: 1 MVAFNLPPLLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLG 60
M AFNLPPLLLSFFL LLQTSTIAAKKSYIVYLGSHSHGLNPSA+DLQLATQTHYNLLG
Sbjct: 1 MEAFNLPPLLLSFFLFVLLQTSTIAAKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWN 120
S+LGSNEAAK+AIFYSYNRHIN FAAVLDQKVAEDIAK PDV+SV+ENKGLKLHTTRSWN
Sbjct: 61 SMLGSNEAAKEAIFYSYNRHINAFAAVLDQKVAEDIAKRPDVVSVYENKGLKLHTTRSWN 120
Query: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
FLGVENDGG+PSNSLWNLS FGESTIIGN+DTGVWPESKSFSD+ YGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPSNSLWNLSRFGESTIIGNLDTGVWPESKSFSDKEYGPIPTRWKGSCEGG 180
Query: 181 FNFSCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFF 240
NF+CNRKLIGARYFNKGY SV P LNSSYET RD NGHGTHTLSTAGGNFVEGVS F
Sbjct: 181 SNFNCNRKLIGARYFNKGYASVAGP--LNSSYETARDDNGHGTHTLSTAGGNFVEGVSIF 240
Query: 241 GNGNGTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTE 300
GNG GTAKGGSPK+LVAAY+VCWP + GGCF+ADILAG EAAISDGVDVLS+SLGG +
Sbjct: 241 GNGYGTAKGGSPKSLVAAYKVCWPPVEDGGCFVADILAGFEAAISDGVDVLSVSLGGRVQ 300
Query: 301 EFSDNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTL 360
EF D+++AIGSFHAVKNGITVVCSAGNSGP+EG+ NVAPW++TVGASTIDRLFTTNVTL
Sbjct: 301 EFYDDILAIGSFHAVKNGITVVCSAGNSGPYEGTASNVAPWMLTVGASTIDRLFTTNVTL 360
Query: 361 GDKRHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVC 420
GDKRHFKG SLS K +PA+KFYP+IRALDAKFNN D + LCL+G+LDP+K +GKIVVC
Sbjct: 361 GDKRHFKGESLSGKILPAKKFYPLIRALDAKFNNVSDYETGLCLEGTLDPKKVEGKIVVC 420
Query: 421 LNWDDIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRS 480
L D+ R++KGYV +AG VGMIL N+ G L+++AH+LPASHISYTDGE V +YI+S
Sbjct: 421 LRGDNSRVDKGYVVAQAGGVGMILANDLLGGRALLSEAHILPASHISYTDGESVYKYIQS 480
Query: 481 NKNPIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSI 540
K P+AY+T V+TELG KPAPIMASFSSRGPN +EP+ILKPDITAPG+NILAAFS
Sbjct: 481 TKTPMAYMTSVRTELGIKPAPIMASFSSRGPNVIEPTILKPDITAPGVNILAAFSNIAPP 540
Query: 541 TGLPYDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLN 600
T +DKRRVL+NVLSGTSM+CPHI+GIVGLLKTLYPKWSPAAI+S IMTTAET+ANDLN
Sbjct: 541 THSHFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETKANDLN 600
Query: 601 PILSSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDT 660
PIL+S +E+A P AYGAGHVQPNKAA+PGLVYDLSTQDYLNFLCA GY+K QM+LF+NDT
Sbjct: 601 PILTSIEEKAGPFAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKTQMRLFTNDT 660
Query: 661 SFVRSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPS 720
SFV SKSFKV DLNYPSISI++L S VNIKRRVKNVGS GTYVA+VE+PPG+ VSVDPS
Sbjct: 661 SFVCSKSFKVTDLNYPSISIFDLRSKEVNIKRRVKNVGSPGTYVAKVEAPPGILVSVDPS 720
Query: 721 TLKFTNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTLQM 772
TLKFT +DEEKDFKVVL+RVPNNQT+ +VFGKLVWSDGKHRVSSPI VTL M
Sbjct: 721 TLKFTKSDEEKDFKVVLKRVPNNQTENHVFGKLVWSDGKHRVSSPISVTLLM 770
BLAST of Csor.00g030640 vs. ExPASy TrEMBL
Match:
A0A6J1KZG2 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111500238 PE=3 SV=1)
HSP 1 Score: 1256 bits (3250), Expect = 0.0
Identity = 630/770 (81.82%), Postives = 687/770 (89.22%), Query Frame = 0
Query: 1 MVAFNLPPLLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLG 60
M AFNLPPLLL FFL ALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQL TQ+HYNLLG
Sbjct: 1 MEAFNLPPLLLPFFLFALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLTTQSHYNLLG 60
Query: 61 SVLGSNEAAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWN 120
SVLGSNEAAK+AIFYSYNR+INGFAAVLDQKVAE +AKHPDVISV+ENKGLKLHTTRSWN
Sbjct: 61 SVLGSNEAAKEAIFYSYNRNINGFAAVLDQKVAEVVAKHPDVISVYENKGLKLHTTRSWN 120
Query: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIP RWKGSCEGG
Sbjct: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPKRWKGSCEGG 180
Query: 181 FNFSCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFF 240
NF CNRKLIGARYFNKGYISVVEPKPLNSSYET RDH+GHGTHTLSTAGGNFVEGVS F
Sbjct: 181 SNFRCNRKLIGARYFNKGYISVVEPKPLNSSYETARDHDGHGTHTLSTAGGNFVEGVSIF 240
Query: 241 GNGNGTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTE 300
GNG GTAKGGSPKALVAAYRVCWP G CF+ADI+AG EAAISDGVDVLS+SLGG+
Sbjct: 241 GNGYGTAKGGSPKALVAAYRVCWPQTLYGACFIADIVAGFEAAISDGVDVLSVSLGGDAT 300
Query: 301 EFSDNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTL 360
EFS++L+AI SFHAVKNGITVVCSAGNSGP E V NVAPW+ITVGASTIDR FTT V L
Sbjct: 301 EFSNDLMAIMSFHAVKNGITVVCSAGNSGPTESIVTNVAPWMITVGASTIDRHFTTYVVL 360
Query: 361 GDKRHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVC 420
G+K KG SLS++ +PA+KFYP+I +LDAK NN DA C KG+LDP+K +GKIVVC
Sbjct: 361 GNKTRLKGESLSNQILPAKKFYPLIHSLDAKLNNVSTYDAQTCEKGALDPKKVQGKIVVC 420
Query: 421 LNWDDIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRS 480
+N D + K YVA +AGA+G+ILVN + G+ L+ AHLLPA+HISYTD + +DQYI+S
Sbjct: 421 VNVGD-PMAKSYVAAQAGAIGIILVNYEDIGDELLPAAHLLPAAHISYTDRKSIDQYIQS 480
Query: 481 NKNPIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSI 540
K+P+AYIT VKTELG KPAPIMASFSSRGPNP+EPSILKPDITAPG+NILAAFS+E S
Sbjct: 481 TKSPMAYITRVKTELGVKPAPIMASFSSRGPNPIEPSILKPDITAPGVNILAAFSDEASP 540
Query: 541 TGLPYDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLN 600
TG P+DKRR+L+ VLSGTSM+CPHI+G+VGLLKTLYPKWSPAAI+S IMTTAET+ANDLN
Sbjct: 541 TGSPFDKRRILYTVLSGTSMSCPHISGVVGLLKTLYPKWSPAAIRSAIMTTAETKANDLN 600
Query: 601 PILSSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDT 660
PIL+ EKE+ NPLAYGAGHVQPNKAA+PGLVYDLSTQDYLNFLCAHGY+K QMKLFSNDT
Sbjct: 601 PILNPEKEKVNPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCAHGYNKTQMKLFSNDT 660
Query: 661 SFVRSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPS 720
SFV SKS KV DLNYPSIS+ NL S V +KRRVKNVGS GTYVAQVE+PPGVSVSVDP+
Sbjct: 661 SFVCSKSVKVTDLNYPSISMNNLKSKEVEVKRRVKNVGSPGTYVAQVEAPPGVSVSVDPN 720
Query: 721 TLKFTNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTL 770
TLKFT T EEKDFKVVLRRVPNNQT VFGKLVW DGKHRVSSPIFVTL
Sbjct: 721 TLKFTKTGEEKDFKVVLRRVPNNQTDGYVFGKLVWYDGKHRVSSPIFVTL 769
BLAST of Csor.00g030640 vs. TAIR 10
Match:
AT5G59810.1 (Subtilase family protein )
HSP 1 Score: 859.4 bits (2219), Expect = 2.3e-249
Identity = 442/766 (57.70%), Postives = 539/766 (70.37%), Query Frame = 0
Query: 5 NLPPLLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLGSVLG 64
+L L L L + A KKSYIVYLGSH+H S+ L +H L S +G
Sbjct: 17 SLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVG 76
Query: 65 SNEAAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWNFLGV 124
S+E AK+AIFYSY RHINGFAA+LD+ A +IAKHPDV+SV NKG KLHTT SWNF+ +
Sbjct: 77 SHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLL 136
Query: 125 ENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGGFNFS 184
+G V +SLWN + +GE TII N+DTGVWPESKSFSDEGYG +P RWKG C +
Sbjct: 137 AKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCHK--DVP 196
Query: 185 CNRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFFGNGN 244
CNRKLIGARYFNKGY++ P N+SYET RDH+GHG+HTLSTA GNFV G + FG GN
Sbjct: 197 CNRKLIGARYFNKGYLAYT-GLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGN 256
Query: 245 GTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTEEFSD 304
GTA GGSPKA VAAY+VCWP + CF ADILA IEAAI DGVDVLS S+GG+ ++
Sbjct: 257 GTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMS 316
Query: 305 NLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTLGDKR 364
+ IAIGSFHAVKNG+TVVCSAGNSGP G+V NVAPW+ITVGAS++DR F V L + +
Sbjct: 317 DGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQ 376
Query: 365 HFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVCLNWD 424
FKG SL SK +P EK Y +I A DA N + DA LC KGSLDP+K KGKI+VCL D
Sbjct: 377 SFKGTSL-SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGD 436
Query: 425 DIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRSNKNP 484
+ R++KG A AGA GM+L N+K SG +I+DAH+LPAS I Y DGE + Y+ S K+P
Sbjct: 437 NARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDP 496
Query: 485 IAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSITGLP 544
YI L TKPAP MASFSSRGPN + P ILKPDITAPG+NI+AAF+E T L
Sbjct: 497 KGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLD 556
Query: 545 YDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLNPILS 604
D RR FN SGTSM+CPHI+G+VGLLKTL+P WSPAAI+S IMTT+ T N P++
Sbjct: 557 SDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVD 616
Query: 605 SEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDTSFVR 664
++ANP +YG+GHVQPNKAA PGLVYDL+T DYL+FLCA GY+ ++LF+ D +
Sbjct: 617 ESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTC 676
Query: 665 SKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPSTLKF 724
+ ++D NYPSI++ NLT + + R++KNVG TY A+ P GV VSV+P L F
Sbjct: 677 RQGANLLDFNYPSITVPNLTGS-ITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTF 736
Query: 725 TNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTL 771
T E K F++ LR +P + VFG+L W+D H V SPI V L
Sbjct: 737 NKTGEVKIFQMTLRPLPVTPS-GYVFGELTWTDSHHYVRSPIVVQL 776
BLAST of Csor.00g030640 vs. TAIR 10
Match:
AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 843.6 bits (2178), Expect = 1.3e-244
Identity = 425/765 (55.56%), Postives = 541/765 (70.72%), Query Frame = 0
Query: 10 LLSFFLCALLQTSTIAAK--KSYIVYLGSHSHGLNPSALDLQLATQTHYNLLGSVLGSNE 69
LL L + +A+K SY+VY G+HSH + + +THY+ LGS GS E
Sbjct: 10 LLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRE 69
Query: 70 AAKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWNFLGVEND 129
A AIFYSY +HINGFAA LD +A +I+KHP+V+SV NK LKLHTTRSW+FLG+E++
Sbjct: 70 RATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHN 129
Query: 130 GGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEG--GFNFSC 189
VPS+S+W + FGE TII N+DTGVWPESKSF DEG GPIP+RWKG C+ F C
Sbjct: 130 SYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHC 189
Query: 190 NRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFFGNGNG 249
NRKLIGARYFNKGY + V LNSS+++ RD +GHG+HTLSTA G+FV GVS FG GNG
Sbjct: 190 NRKLIGARYFNKGYAAAV--GHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNG 249
Query: 250 TAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTEEFSDN 309
TAKGGSP+A VAAY+VCWP + C+ AD+LA +AAI DG DV+S+SLGG F ++
Sbjct: 250 TAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFND 309
Query: 310 LIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTLGDKRH 369
+AIGSFHA K I VVCSAGNSGP + +V NVAPW ITVGAST+DR F +N+ LG+ +H
Sbjct: 310 SVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKH 369
Query: 370 FKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVCLNWDD 429
+KG SLSS +P KFYPI+ +++AK N+ +DA LC GSLDP K KGKI+VCL +
Sbjct: 370 YKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQN 429
Query: 430 IRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRSNKNPI 489
R+EKG G +GM+L N +G L+AD H+LPA+ ++ D V +YI K PI
Sbjct: 430 GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPI 489
Query: 490 AYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSITGLPY 549
A+ITP +T+LG KPAP+MASFSS+GP+ V P ILKPDITAPG++++AA++ S T +
Sbjct: 490 AHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQF 549
Query: 550 DKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLNPILSS 609
D RR+LFN +SGTSM+CPHI+GI GLLKT YP WSPAAI+S IMTTA + PI ++
Sbjct: 550 DPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNA 609
Query: 610 EKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDTSFVRS 669
+A P ++GAGHVQPN A +PGLVYDL +DYLNFLC+ GY+ Q+ +FS + S
Sbjct: 610 TNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSS 669
Query: 670 KSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVESPPGVSVSVDPSTLKFT 729
+++LNYPSI++ NLTS V + R VKNVG Y +V +P GV V+V P++L FT
Sbjct: 670 PKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFT 729
Query: 730 NTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVTL 771
E+K FKV+L + N K VFG+LVWSD KHRV SPI V L
Sbjct: 730 KVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772
BLAST of Csor.00g030640 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 617.5 bits (1591), Expect = 1.5e-176
Identity = 342/767 (44.59%), Postives = 480/767 (62.58%), Query Frame = 0
Query: 9 LLLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLGSVLGSNEA 68
LLL C + +S+ + + +YIV++ PS+ DL H N S L S
Sbjct: 13 LLLCLGFCHV--SSSSSDQGTYIVHMAKSQ---MPSSFDL------HSNWYDSSLRSISD 72
Query: 69 AKKAIFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWNFLGVENDG 128
+ + + Y+Y I+GF+ L Q+ A+ + P VISV +LHTTR+ FLG++
Sbjct: 73 SAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH- 132
Query: 129 GVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGGFNFS---C 188
+ L+ + ++G +DTGVWPESKS+SDEG+GPIP+ WKG CE G NF+ C
Sbjct: 133 ---TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLC 192
Query: 189 NRKLIGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFFGNGNG 248
NRKLIGAR+F +GY S + P + + RD +GHGTHT STA G+ VEG S G +G
Sbjct: 193 NRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASG 252
Query: 249 TAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTEEFSDN 308
TA+G +P+A VA Y+VCW GGCF +DILA I+ AI+D V+VLS+SLGG ++ +
Sbjct: 253 TARGMAPRARVAVYKVCW----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRD 312
Query: 309 LIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTLGDKRH 368
+AIG+F A++ GI V CSAGN+GP S+ NVAPWI TVGA T+DR F LG+ ++
Sbjct: 313 GVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKN 372
Query: 369 FKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVCLNWDD 428
F G+SL +K P I A N S+ + LC+ G+L PEK KGKIV+C +
Sbjct: 373 FTGVSLFKGEALPDKLLPFIYA----GNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN 432
Query: 429 IRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRSNKNPI 488
R++KG V AG VGMIL N +GE L+ADAHLLPA+ + G+++ Y+ ++ NP
Sbjct: 433 ARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPT 492
Query: 489 AYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSITGLPY 548
A I+ + T +G KP+P++A+FSSRGPN + P+ILKPD+ APG+NILAA++ TGL
Sbjct: 493 ASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLAS 552
Query: 549 DKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLNPILS- 608
D RRV FN++SGTSM+CPH++G+ LLK+++P+WSPAAI+S +MTTA D P+L
Sbjct: 553 DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI 612
Query: 609 SEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFS-NDTSFV 668
+ + + P +GAGHV P A +PGL+YDL+T+DYL FLCA Y Q++ S + +
Sbjct: 613 ATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCD 672
Query: 669 RSKSFKVIDLNYPSISIYNLTSDVVNIKRRVKNVGSSGTYVAQVES-PPGVSVSVDPSTL 728
SKS+ V DLNYPS ++ R V +VG +GTY +V S GV +SV+P+ L
Sbjct: 673 PSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVL 732
Query: 729 KFTNTDEEKDFKVVLRRVPNNQTKENVFGKLVWSDGKHRVSSPIFVT 770
F +E+K + V + + N FG + WSDGKH V SP+ ++
Sbjct: 733 NFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755
BLAST of Csor.00g030640 vs. TAIR 10
Match:
AT2G05920.1 (Subtilase family protein )
HSP 1 Score: 565.8 bits (1457), Expect = 5.2e-161
Identity = 328/767 (42.76%), Postives = 457/767 (59.58%), Query Frame = 0
Query: 10 LLSFFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLGSVLGSNEAA 69
+++ FL LL T+ AKK+YI+ + +H P + TH++ S L S
Sbjct: 13 IITTFLFLLLHTT---AKKTYIIRV---NHSDKPESF------LTHHDWYTSQLNS---- 72
Query: 70 KKAIFYSYNRHINGFAAVLDQKVAED-IAKHPDVISVHENKGLKLHTTRSWNFLGVENDG 129
+ ++ Y+Y +GF+A LD A+ ++ ++ + E+ LHTTR+ FLG+ ++
Sbjct: 73 ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEF 132
Query: 130 GVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGGFNFS---C 189
GV +L S IIG +DTGVWPES+SF D IP++WKG CE G +F C
Sbjct: 133 GV-----HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLC 192
Query: 190 NRKLIGARYFNKGYISVVEPKPLNSSYETT--RDHNGHGTHTLSTAGGNFVEGVSFFGNG 249
N+KLIGAR F+KG+ + +S E+ RD +GHGTHT +TA G+ V SF G
Sbjct: 193 NKKLIGARSFSKGF-QMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYA 252
Query: 250 NGTAKGGSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTEEFS 309
GTA+G + +A VA Y+VCW S GCF +DILA ++ AI DGVDVLS+SLGG + +
Sbjct: 253 AGTARGMATRARVATYKVCW----STGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYY 312
Query: 310 DNLIAIGSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTLGDK 369
+ IAIG+F A++ G+ V CSAGNSGP SV NVAPW++TVGA T+DR F LG+
Sbjct: 313 RDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNG 372
Query: 370 RHFKGISLSSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVCLNW 429
+ G+SL S K L+ +N + + LCL GSLD +GKIVVC
Sbjct: 373 KRLTGVSLYSGVGMGTK------PLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRG 432
Query: 430 DDIRLEKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYIRSNKN 489
+ R+EKG V +AG +GMI+ N SGE L+AD+HLLPA + G+L+ +Y++S+
Sbjct: 433 VNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSK 492
Query: 490 PIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSITGL 549
P A + T L KP+P++A+FSSRGPN V P ILKPD+ PG+NILA +S+ TGL
Sbjct: 493 PTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGL 552
Query: 550 PYDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLNPIL 609
D RR FN++SGTSM+CPHI+G+ GLLK +P+WSP+AIKS +MTTA N P+
Sbjct: 553 DKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLH 612
Query: 610 -SSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSNDTSF 669
+++ +NP A+G+GHV P KA PGLVYD+ST++Y+ FLC+ Y + + S
Sbjct: 613 DAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSV 672
Query: 670 VRSKSFK-VIDLNYPSISIYNLTSDVVNIKRRVKNVG-SSGTYVAQVESPPGVSVSVDPS 729
SK F LNYPS S+ VV R V NVG +S Y V P V +SV PS
Sbjct: 673 NCSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPS 732
Query: 730 TLKFTNTDEEKDFKVV-LRRVPNNQTKENVFGKLVWSDGKHRVSSPI 767
L F + E+K + V + + + T + FG + WS+ +H V SP+
Sbjct: 733 KLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPV 747
BLAST of Csor.00g030640 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 559.3 bits (1440), Expect = 4.8e-159
Identity = 326/784 (41.58%), Postives = 453/784 (57.78%), Query Frame = 0
Query: 13 FFLCALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLGSVLGSNEAAKKA 72
FFL L S+ A+ + + Y+ H PS TH++ S L S ++ +
Sbjct: 8 FFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIF------PTHFHWYTSSLASLTSSPPS 67
Query: 73 IFYSYNRHINGFAAVLDQKVAEDIAKHPDVISVHENKGLKLHTTRSWNFLGVENDGGVPS 132
I ++Y+ +GF+A L + A + HP VISV + LHTTRS FLG+ +
Sbjct: 68 IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRS---TDK 127
Query: 133 NSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGGFNF---SCNRKL 192
L S FG +IG IDTGVWPE SF D G GP+P +WKG C +F +CNRKL
Sbjct: 128 AGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKL 187
Query: 193 IGARYFNKGYISVVEPKPLNSSYETTRDHNGHGTHTLSTAGGNFVEGVSFFGNGNGTAKG 252
+GAR+F GY + + + + RD +GHGTHT S + G +V S G +G A G
Sbjct: 188 VGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAG 247
Query: 253 GSPKALVAAYRVCWPSMGSGGCFMADILAGIEAAISDGVDVLSISLGGNTEEFSDNLIAI 312
+PKA +AAY+VCW S GC+ +DILA + A++DGVDV+S+S+GG + + IAI
Sbjct: 248 MAPKARLAAYKVCWNS----GCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAI 307
Query: 313 GSFHAVKNGITVVCSAGNSGPFEGSVENVAPWIITVGASTIDRLFTTNVTLGDKRHFKGI 372
G+F A+ GI V SAGN GP +V NVAPW+ TVGA TIDR F NV LG+ + G+
Sbjct: 308 GAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGV 367
Query: 373 SL-SSKTMPAEKFYPIIRALDAKFNNSHDIDAALCLKGSLDPEKAKGKIVVCLNWDDIRL 432
S+ + + YP++ ++LCL+GSLDP KGKIV+C + R
Sbjct: 368 SVYGGPGLDPGRMYPLV--YGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRA 427
Query: 433 EKGYVADEAGAVGMILVNNKGSGEGLIADAHLLPASHISYTDGELVDQYI------RSNK 492
KG + + G +GMI+ N GEGL+AD H+LPA+ + + G+ + +YI RS+K
Sbjct: 428 TKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSK 487
Query: 493 NPIAYITPVKTELGTKPAPIMASFSSRGPNPVEPSILKPDITAPGINILAAFSEENSITG 552
+P A I T LG +PAP++ASFS+RGPNP P ILKPD+ APG+NILAA+ + +G
Sbjct: 488 HPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSG 547
Query: 553 LPYDKRRVLFNVLSGTSMACPHIAGIVGLLKTLYPKWSPAAIKSVIMTTAETEANDLNPI 612
+ D RR FN+LSGTSMACPH++G+ LLK +P WSPAAI+S ++TTA T N P+
Sbjct: 548 VTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPM 607
Query: 613 L-SSEKERANPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCAHGYDKEQMKLFSN--- 672
+ S ++ + YG+GHV P KA DPGLVYD+++ DY+NFLC Y + + +
Sbjct: 608 MDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA 667
Query: 673 DTSFVRSKSFKVIDLNYPSISI----YNLTSDVVNIKRRVKNVG-SSGTYVAQVESPPGV 732
D R ++ V +LNYPS S+ Y + + R V NVG S Y ++ P G
Sbjct: 668 DCDGAR-RAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGT 727
Query: 733 SVSVDPSTLKFTNTDEEKDFKVVLRRVPNNQTK-----ENV-FGKLVWSDGKHRVSSPIF 772
+V+V+P L F ++ F V RV + K NV G +VWSDGK V+SP+
Sbjct: 728 TVTVEPEKLSFRRVGQKLSFVV---RVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLV 772
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| F4JXC5 | 3.2e-248 | 57.70 | Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... | [more] |
| Q9ZSP5 | 1.8e-243 | 55.56 | Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... | [more] |
| I1N462 | 8.2e-204 | 50.13 | Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... | [more] |
| O65351 | 2.1e-175 | 44.59 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| Q9ZUF6 | 7.3e-160 | 42.76 | Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| KAG6577535.1 | 0.0 | 100.00 | Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| XP_023552945.1 | 0.0 | 96.24 | subtilisin-like protease SBT5.4 [Cucurbita pepo subsp. pepo] | [more] |
| KAG6577554.1 | 0.0 | 82.77 | Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| KAG6577555.1 | 0.0 | 82.25 | Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| KAG6577557.1 | 0.0 | 81.22 | Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1EWJ2 | 0.0 | 82.51 | subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111438754 PE... | [more] |
| A0A6J1EW82 | 0.0 | 81.09 | subtilisin-like protease SBT5.3 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC1... | [more] |
| A0A6J1EWV1 | 0.0 | 80.83 | subtilisin-like protease SBT5.3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... | [more] |
| A0A6J1F1I2 | 0.0 | 80.83 | subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1... | [more] |
| A0A6J1KZG2 | 0.0 | 81.82 | subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111500238 PE=3... | [more] |
