Homology
BLAST of Csor.00g030610 vs. ExPASy Swiss-Prot
Match:
F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)
HSP 1 Score: 875.9 bits (2262), Expect = 3.3e-253
Identity = 450/769 (58.52%), Postives = 547/769 (71.13%), Query Frame = 0
Query: 1 MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
M +L LLL L L + A+KKSYIVYLGSH+H S+ L +H L
Sbjct: 16 MSLQSLSSLLL---LVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLA 75
Query: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWS 120
S +GS+E AKEAIFYSY RHINGFAA+LD A ++AKHP V+SV NKGR+LHTT+SW+
Sbjct: 76 SFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWN 135
Query: 121 FLGVENNGELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGG 180
F+ + NG + +SLWN A +GE TII NLDTG+WPESKSFSDEGYG +P RWKG C
Sbjct: 136 FMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH-- 195
Query: 181 SNFSCNRKLIGARYFNKGYASIVG-PLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFG 240
+ CNRKLIGARYFNKGY + G P N+SY T RD GHG+HTLSTA G+FV AN+FG
Sbjct: 196 KDVPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFG 255
Query: 241 NGNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLE 300
GNGTA GGSPKA VAAYKVCWP V CFD+D+LAA EAAI DGVDVLS S+GG +
Sbjct: 256 IGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGD 315
Query: 301 FHDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALG 360
+ D IAIGSFHAVKNG+TVVCSAGNSGP G+V+NVAPW+ITVGAS+ DR F +V L
Sbjct: 316 YMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELK 375
Query: 361 DKRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCL 420
+ + KG S+S K LP +K Y LI A DA N + DA+LC +GSLDPKKV+ KI+VCL
Sbjct: 376 NGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCL 435
Query: 421 SGDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYINST 480
GD V+KG A AGA GM+L NDK G+ + DAH+LPAS I+Y DGE ++ Y++ST
Sbjct: 436 RGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSST 495
Query: 481 KTPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPT 540
K P Y+ L KPAP M FSSRGPN I P ILKPDITAPGVNI+AAFTE T PT
Sbjct: 496 KDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPT 555
Query: 541 HSSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKANDLNP 600
D RR PFN SGTSMSCPHISG+VGLLKTL+P WSPA ++SAIMTT+RT+ N P
Sbjct: 556 DLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKP 615
Query: 601 ILSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTS 660
++ +KAN F+YG+GHV PNKAA+PGLVYDL+T DYL+FLCA GYN T ++LF+ D
Sbjct: 616 MVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQ 675
Query: 661 FVCSKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPRVSISVEPNT 720
+ C + + D NYPSIT+ NL + + R++KN+G P+TY A + P V +SVEP
Sbjct: 676 YTCRQGANLLDFNYPSITVPNLTGSIT-VTRKLKNVGPPATYNARFREPLGVRVSVEPKQ 735
Query: 721 LKFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVL 769
L F +TGE K F + L+ +P G VFG L W++ H+VRSPI+V L
Sbjct: 736 LTFNKTGEVKIFQMTLRPLPVT-PSGYVFGELTWTDSHHYVRSPIVVQL 776
BLAST of Csor.00g030610 vs. ExPASy Swiss-Prot
Match:
Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)
HSP 1 Score: 857.8 bits (2215), Expect = 9.2e-248
Identity = 424/742 (57.14%), Postives = 531/742 (71.56%), Query Frame = 0
Query: 29 SYIVYLGSHSHGLNPSAIDLQLATQTHYNLLGSVLGSNEAAKEAIFYSYNRHINGFAAVL 88
SY+VY G+HSH + + +THY+ LGS GS E A +AIFYSY +HINGFAA L
Sbjct: 31 SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90
Query: 89 DHKVAEDVAKHPSVISVHENKGRELHTTNSWSFLGVENNGELPLNSLWNLANFGESTIIG 148
DH +A +++KHP V+SV NK +LHTT SW FLG+E+N +P +S+W A FGE TII
Sbjct: 91 DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150
Query: 149 NLDTGIWPESKSFSDEGYGPIPKRWKGSCEG--GSNFSCNRKLIGARYFNKGYASIVGPL 208
NLDTG+WPESKSF DEG GPIP RWKG C+ + F CNRKLIGARYFNKGYA+ VG L
Sbjct: 151 NLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHL 210
Query: 209 NSSYATARDDQGHGTHTLSTAGGHFVSRANIFGNGNGTAKGGSPKALVAAYKVCWPRVKV 268
NSS+ + RD GHG+HTLSTA G FV +IFG GNGTAKGGSP+A VAAYKVCWP VK
Sbjct: 211 NSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKG 270
Query: 269 GGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEFHDDPIAIGSFHAVKNGITVVCSAGNS 328
C+D+DVLAAF+AAI DG DV+S+SLGG P F +D +AIGSFHA K I VVCSAGNS
Sbjct: 271 NECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNS 330
Query: 329 GPSPGSVANVAPWLITVGASTTDRLFTTYVALGDKRHIKGESISNKILPVQKFYPLIRAL 388
GP+ +V+NVAPW ITVGAST DR F + + LG+ +H KG+S+S+ LP KFYP++ ++
Sbjct: 331 GPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASV 390
Query: 389 DAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCLSGDITNVEKGYVVAQAGAVGMILANDK 448
+AK N S +DA LC GSLDP K + KI+VCL G VEKG VA G +GM+L N
Sbjct: 391 NAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTY 450
Query: 449 EDGDRLEVDAHLLPASNINYIDGELVYQYINSTKTPMAYMTRVRTELGVKPAPVMGRFSS 508
G+ L D H+LPA+ + D V +YI+ TK P+A++T RT+LG+KPAPVM FSS
Sbjct: 451 VTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSS 510
Query: 509 RGPNIIEPSILKPDITAPGVNILAAFTEETSPTHSSFDKRRVPFNILSGTSMSCPHISGI 568
+GP+I+ P ILKPDITAPGV+++AA+T SPT+ FD RR+ FN +SGTSMSCPHISGI
Sbjct: 511 KGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGI 570
Query: 569 VGLLKTLYPKWSPAVMKSAIMTTARTKANDLNPILSSGKEKANSFAYGAGHVNPNKAANP 628
GLLKT YP WSPA ++SAIMTTA + PI ++ KA F++GAGHV PN A NP
Sbjct: 571 AGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNP 630
Query: 629 GLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSFVCSKKFKVTDLNYPSITIDNLASEVV 688
GLVYDL +DYLNFLC+ GYN +Q+ +FS + S K + +LNYPSIT+ NL S V
Sbjct: 631 GLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKV 690
Query: 689 KIKRRVKNLGCPSTYVANVKAPPRVSISVEPNTLKFTRTGEEKSFTVVLKRVPNDYHRGA 748
+ R VKN+G PS Y V P V ++V+P +L FT+ GE+K+F V+L + + +G
Sbjct: 691 TVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGY 750
Query: 749 VFGRLAWSNGKHHVRSPILVVL 769
VFG L WS+ KH VRSPI+V L
Sbjct: 751 VFGELVWSDKKHRVRSPIVVKL 772
BLAST of Csor.00g030610 vs. ExPASy Swiss-Prot
Match:
I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)
HSP 1 Score: 729.9 bits (1883), Expect = 2.9e-209
Identity = 402/786 (51.15%), Postives = 515/786 (65.52%), Query Frame = 0
Query: 4 FNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLGSVL 63
F L +L SFFLF L + KK YIVY+G+HSHG +P++ DL+LAT +HY+LLGS+
Sbjct: 6 FCLHLILSSFFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIF 65
Query: 64 GSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWSFLG 123
GS E AKEAI YSYNRHINGFAA+L+ + A D+AK+P+V+SV +K +LHTT SW FLG
Sbjct: 66 GSREKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLG 125
Query: 124 VENNGELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGS-CE---- 183
+ G+ NS W FGE+TIIGN+DTG+WPES+SFSD+GYG +P +W+G C+
Sbjct: 126 LHRRGQ---NSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKL 185
Query: 184 -GGSNFSCNRKLIGARYFNKGYASIVGPLNSSYATARDDQGHGTHTLSTAGGHFVSRANI 243
G +CNRKLIGARY+NK + + G L+ TARD GHGTHTLSTAGG+FV A +
Sbjct: 186 PGSMKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARV 245
Query: 244 FGNGNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSP 303
F GNGTAKGGSP+A VAAYKVCW C+ +DVLAA + AI DGVDV+++S G S
Sbjct: 246 FAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSY 305
Query: 304 L----EFHDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFT 363
+ D I+IG+FHA+ I +V SAGN GP+PG+VANVAPW+ T+ AST DR F+
Sbjct: 306 VVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFS 365
Query: 364 TYVALGDKRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEE 423
+ + + ++ I+G S+ + P Q F LI + DAK N + DA LC G+LD KV
Sbjct: 366 SNLTINNQL-IEGASLFVNLPPNQAF-SLILSTDAKLANATFRDAQLCRRGTLDRTKVNG 425
Query: 424 KIVVCL-SGDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNI-------- 483
KIV+C G I +V +G AGA GMIL N ++G L + H+ N
Sbjct: 426 KIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSR 485
Query: 484 -NYIDGELVYQYINSTKT-PMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDIT 543
+ + + + KT M+R RT G KPAPVM FSSRGPN I+PSILKPD+T
Sbjct: 486 PHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVT 545
Query: 544 APGVNILAAFTEETSPTHSSFDKRR-VPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAV 603
APGVNILAA++E S + D RR FN+L GTSMSCPH SGI GLLKT +P WSPA
Sbjct: 546 APGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAA 605
Query: 604 MKSAIMTTARTKANDLNPILSS-GKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNF 663
+KSAIMTTA T N PI + K A++FAYG+GHV P+ A PGLVYDLS DYLNF
Sbjct: 606 IKSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNF 665
Query: 664 LCARGYNKTQMKLFSNDTSFVCSKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPST 723
LCA GY++ + + + +F+CS V DLNYPSIT+ NL + V I R V N+G PST
Sbjct: 666 LCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPST 725
Query: 724 YVANVKAPPRVSISVEPNTLKFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHV 767
Y + ++P SI+V P +L FT+ GE K+F V+++ R FG L W++GKH V
Sbjct: 726 YTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIV 785
BLAST of Csor.00g030610 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 610.9 bits (1574), Expect = 2.0e-173
Identity = 351/774 (45.35%), Postives = 476/774 (61.50%), Query Frame = 0
Query: 12 SFFLFALL----QTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLGSVLGSNE 71
+FFL L +S+ + + +YIV++ PS+ DL H N S L S
Sbjct: 10 AFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQ---MPSSFDL------HSNWYDSSLRSIS 69
Query: 72 AAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWSFLGVENN 131
+ E + Y+Y I+GF+ L + A+ + P VISV ELHTT + FLG++ +
Sbjct: 70 DSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH 129
Query: 132 GELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGGSNFS--- 191
L+ A ++G LDTG+WPESKS+SDEG+GPIP WKG CE G+NF+
Sbjct: 130 ----TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASL 189
Query: 192 CNRKLIGARYFNKGYASIVGPLNSS--YATARDDQGHGTHTLSTAGGHFVSRANIFGNGN 251
CNRKLIGAR+F +GY S +GP++ S + RDD GHGTHT STA G V A++ G +
Sbjct: 190 CNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYAS 249
Query: 252 GTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEFHD 311
GTA+G +P+A VA YKVCW +GGCF SD+LAA + AI+D V+VLS+SLGG +++
Sbjct: 250 GTARGMAPRARVAVYKVCW----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYR 309
Query: 312 DPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALGDKR 371
D +AIG+F A++ GI V CSAGN+GPS S++NVAPW+ TVGA T DR F LG+ +
Sbjct: 310 DGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGK 369
Query: 372 H------IKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIV 431
+ KGE++ +K+LP F A +A N N LC+ G+L P+KV+ KIV
Sbjct: 370 NFTGVSLFKGEALPDKLLP---FIYAGNASNATNGN-------LCMTGTLIPEKVKGKIV 429
Query: 432 VCLSGDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYI 491
+C G V+KG VV AG VGMILAN +G+ L DAHLLPA+ + G+++ Y+
Sbjct: 430 MCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYV 489
Query: 492 NSTKTPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEET 551
+ P A ++ + T +GVKP+PV+ FSSRGPN I P+ILKPD+ APGVNILAA+T
Sbjct: 490 TTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAA 549
Query: 552 SPTHSSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKAND 611
PT + D RRV FNI+SGTSMSCPH+SG+ LLK+++P+WSPA ++SA+MTTA D
Sbjct: 550 GPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKD 609
Query: 612 LNPILSSGKEKANS-FAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFS 671
P+L K ++ F +GAGHV+P A NPGL+YDL+TEDYL FLCA Y Q++ S
Sbjct: 610 GKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVS 669
Query: 672 NDTSFVC--SKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPR-VS 731
++ C SK + V DLNYPS ++ K R V ++G TY V + V
Sbjct: 670 R-RNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVK 729
Query: 732 ISVEPNTLKFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILV 767
ISVEP L F E+KS+TV + FG + WS+GKH V SP+ +
Sbjct: 730 ISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754
BLAST of Csor.00g030610 vs. ExPASy Swiss-Prot
Match:
Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)
HSP 1 Score: 580.1 bits (1494), Expect = 3.7e-164
Identity = 351/788 (44.54%), Postives = 473/788 (60.03%), Query Frame = 0
Query: 8 PLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLGSVLGSNE 67
PLLL FF +S+ +SYIV++ SH PS H +LL S+ S +
Sbjct: 14 PLLLCFF---SPSSSSSDGLESYIVHV-QRSH--KPSLFSSH--NNWHVSLLRSLPSSPQ 73
Query: 68 AAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWSFLGVENN 127
A + YSY+R ++GF+A L + +HPSVISV ++ RE+HTT++ +FLG N
Sbjct: 74 PA--TLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQN 133
Query: 128 GELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGGSNF---S 187
+ LW+ +N+GE I+G LDTGIWPE SFSD G GPIP WKG CE G +F S
Sbjct: 134 -----SGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASS 193
Query: 188 CNRKLIGARYFNKGYASIVGPLNSSYA----TARDDQGHGTHTLSTAGGHFVSRANIFGN 247
CNRKLIGAR F +GY + A + RD +GHGTHT STA G V+ A+++
Sbjct: 194 CNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQY 253
Query: 248 GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLG--GSPL 307
GTA G + KA +AAYK+CW GGC+DSD+LAA + A++DGV V+S+S+G GS
Sbjct: 254 ARGTATGMASKARIAAYKICW----TGGCYDSDILAAMDQAVADGVHVISLSVGASGSAP 313
Query: 308 EFHDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVAL 367
E+H D IAIG+F A ++GI V CSAGNSGP+P + N+APW++TVGAST DR F
Sbjct: 314 EYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAIT 373
Query: 368 GDKRHIKGESI-SNKILPVQKFYPLIRALDAKNNNV--SDIDAVLCLEGSLDPKKVEEKI 427
GD + G S+ + + LP D++ + V D + LC G L+ VE KI
Sbjct: 374 GDGKVFTGTSLYAGESLP-----------DSQLSLVYSGDCGSRLCYPGKLNSSLVEGKI 433
Query: 428 VVCLSGDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQY 487
V+C G VEKG V AG GMILAN E G+ L D+HL+PA+ + G+ + Y
Sbjct: 434 VLCDRGGNARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDY 493
Query: 488 INSTKTPMAYMTRVRTELGVK-PAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTE 547
I ++ +P A ++ + T +G P+P + FSSRGPN + P ILKPD+ APGVNILA +T
Sbjct: 494 IKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTG 553
Query: 548 ETSPTHSSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKA 607
PT D RRV FNI+SGTSMSCPH+SG+ LL+ +P WSPA +KSA++TTA
Sbjct: 554 MVGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVE 613
Query: 608 NDLNPI--LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMK 667
N PI L++GK +NSF +GAGHV+PNKA NPGLVYD+ ++Y+ FLCA GY +
Sbjct: 614 NSGEPIEDLATGK-SSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGIL 673
Query: 668 LFSNDTSFV----CSKKFKVTDLNYPSITIDNLAS-EVVKIKRRVKNLG--CPSTYVANV 727
+F D + SK DLNYPS ++ ++ EVVK KR VKN+G + Y V
Sbjct: 674 VFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGV 733
Query: 728 KAPPRVSISVEPNTLKFTRTGEEKSFTVVLKRVPNDYHRGAV----FGRLAWSNGKHHVR 770
K+P V I V P+ L F++ + V K V G+V FG + W++G+H V+
Sbjct: 734 KSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVK 770
BLAST of Csor.00g030610 vs. NCBI nr
Match:
KAG6577538.1 (Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1541 bits (3990), Expect = 0.0
Identity = 769/769 (100.00%), Postives = 769/769 (100.00%), Query Frame = 0
Query: 1 MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG
Sbjct: 1 MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWS 120
SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWS
Sbjct: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWS 120
Query: 121 FLGVENNGELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGG 180
FLGVENNGELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGG
Sbjct: 121 FLGVENNGELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGG 180
Query: 181 SNFSCNRKLIGARYFNKGYASIVGPLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFGN 240
SNFSCNRKLIGARYFNKGYASIVGPLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFGN
Sbjct: 181 SNFSCNRKLIGARYFNKGYASIVGPLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFGN 240
Query: 241 GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEF 300
GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEF
Sbjct: 241 GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEF 300
Query: 301 HDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALGD 360
HDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALGD
Sbjct: 301 HDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALGD 360
Query: 361 KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCLS 420
KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCLS
Sbjct: 361 KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCLS 420
Query: 421 GDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYINSTK 480
GDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYINSTK
Sbjct: 421 GDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYINSTK 480
Query: 481 TPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTH 540
TPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTH
Sbjct: 481 TPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTH 540
Query: 541 SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKANDLNPI 600
SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKANDLNPI
Sbjct: 541 SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKANDLNPI 600
Query: 601 LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF 660
LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF
Sbjct: 601 LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF 660
Query: 661 VCSKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPRVSISVEPNTL 720
VCSKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPRVSISVEPNTL
Sbjct: 661 VCSKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPRVSISVEPNTL 720
Query: 721 KFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVLG 769
KFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVLG
Sbjct: 721 KFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVLG 769
BLAST of Csor.00g030610 vs. NCBI nr
Match:
XP_023553312.1 (subtilisin-like protease SBT5.4 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1508 bits (3903), Expect = 0.0
Identity = 745/769 (96.88%), Postives = 758/769 (98.57%), Query Frame = 0
Query: 1 MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
MEAFNLPPLLLSFFLFAL QTSTIA+KKSYIVYLGSHSHGLNPSA+DLQLATQTHYNLLG
Sbjct: 1 MEAFNLPPLLLSFFLFALFQTSTIALKKSYIVYLGSHSHGLNPSAVDLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWS 120
SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAED+AKHPSV+SVHENKGRELH TNSWS
Sbjct: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDIAKHPSVVSVHENKGRELHATNSWS 120
Query: 121 FLGVENNGELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGG 180
FLGVENNGELP NSLWNLANFGESTIIGNLDTG+WPESKSFSDEGYGPIPKRWKGSCEGG
Sbjct: 121 FLGVENNGELPPNSLWNLANFGESTIIGNLDTGVWPESKSFSDEGYGPIPKRWKGSCEGG 180
Query: 181 SNFSCNRKLIGARYFNKGYASIVGPLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFGN 240
SNFSCNRKLIGARYFNKGYASIVGPLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFGN
Sbjct: 181 SNFSCNRKLIGARYFNKGYASIVGPLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFGN 240
Query: 241 GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEF 300
GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLA FEAAISDGVDVLSISLGGSPLEF
Sbjct: 241 GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAGFEAAISDGVDVLSISLGGSPLEF 300
Query: 301 HDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALGD 360
HDDPIAI SFHAVKNGITVVCSAGNSGPSPG+VANVAPWLITVGASTTDRLFTTYVALGD
Sbjct: 301 HDDPIAIASFHAVKNGITVVCSAGNSGPSPGTVANVAPWLITVGASTTDRLFTTYVALGD 360
Query: 361 KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCLS 420
KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDI+AVLCLEGSLDPKKVEEKIVVCLS
Sbjct: 361 KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIEAVLCLEGSLDPKKVEEKIVVCLS 420
Query: 421 GDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYINSTK 480
GDITNVEKGYV AQAGAVGMILAND+EDGDRLEVDAHLLPASNINY+DGELVYQYINSTK
Sbjct: 421 GDITNVEKGYVAAQAGAVGMILANDEEDGDRLEVDAHLLPASNINYVDGELVYQYINSTK 480
Query: 481 TPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTH 540
TPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTH
Sbjct: 481 TPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTH 540
Query: 541 SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKANDLNPI 600
SFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPA+MKSAIMTTARTKANDLNPI
Sbjct: 541 LSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAIMKSAIMTTARTKANDLNPI 600
Query: 601 LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF 660
L+SGKEKANSFAYGAGHVNPNKAANPGLVYDLS EDYLNFLCARGYNKTQMKLFSNDTSF
Sbjct: 601 LNSGKEKANSFAYGAGHVNPNKAANPGLVYDLSIEDYLNFLCARGYNKTQMKLFSNDTSF 660
Query: 661 VCSKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPRVSISVEPNTL 720
VCSKKFKVTDLNYPSITIDNL SEVVKIKRRVKN+G P TYVANVKAPPRVS+SVEPNTL
Sbjct: 661 VCSKKFKVTDLNYPSITIDNLTSEVVKIKRRVKNVGSPGTYVANVKAPPRVSVSVEPNTL 720
Query: 721 KFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVLG 769
KFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVLG
Sbjct: 721 KFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVLG 769
BLAST of Csor.00g030610 vs. NCBI nr
Match:
XP_023007710.1 (subtilisin-like protease SBT5.4 [Cucurbita maxima])
HSP 1 Score: 1471 bits (3807), Expect = 0.0
Identity = 726/769 (94.41%), Postives = 749/769 (97.40%), Query Frame = 0
Query: 1 MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSA+DLQLATQTHYNLLG
Sbjct: 1 MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAVDLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWS 120
SV+GSNEAAKEAIFYSYNRHINGFAAVLD+KVAEDVAKHPSV+SVHENKGRELHTTNSWS
Sbjct: 61 SVIGSNEAAKEAIFYSYNRHINGFAAVLDYKVAEDVAKHPSVVSVHENKGRELHTTNSWS 120
Query: 121 FLGVENNGELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGG 180
FLGVENNG LP NSLWNLANFGESTIIGNLDTGIWPESKSF+DEGYGPIPKRWKGSCEGG
Sbjct: 121 FLGVENNGGLPPNSLWNLANFGESTIIGNLDTGIWPESKSFNDEGYGPIPKRWKGSCEGG 180
Query: 181 SNFSCNRKLIGARYFNKGYASIVGPLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFGN 240
SNF+CNRKLIGARYFNKGYASIVGPLNSSY TARDDQGHGTHTLSTAGGHFVSRANIFGN
Sbjct: 181 SNFNCNRKLIGARYFNKGYASIVGPLNSSYLTARDDQGHGTHTLSTAGGHFVSRANIFGN 240
Query: 241 GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEF 300
GNGTAKGGSPKALVAAYKVCWPRV +GGCFDSD+LA FEAAISDGVDVLSISLGGSPLEF
Sbjct: 241 GNGTAKGGSPKALVAAYKVCWPRVNIGGCFDSDILAGFEAAISDGVDVLSISLGGSPLEF 300
Query: 301 HDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALGD 360
HDDPIAI SFHAVKNGITVVCSAGNSGPSPG+VANVAPWLITVGAST DRLFTTY+ALGD
Sbjct: 301 HDDPIAIASFHAVKNGITVVCSAGNSGPSPGTVANVAPWLITVGASTIDRLFTTYLALGD 360
Query: 361 KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCLS 420
KRHIKGESISNKILPVQKFYPLIRALDAKNNNVS+I+A+LCLEGSLDPKKVE KIVVCLS
Sbjct: 361 KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSNIEAILCLEGSLDPKKVEGKIVVCLS 420
Query: 421 GDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYINSTK 480
GDI NV+KGYV AQAGAVGMIL NDKEDGDRLEV+AHLLP S+INYIDGELVYQ+INSTK
Sbjct: 421 GDIANVDKGYVAAQAGAVGMILTNDKEDGDRLEVEAHLLPTSHINYIDGELVYQFINSTK 480
Query: 481 TPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTH 540
TP+AYMT VRTELGVKPAP+M RFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPT
Sbjct: 481 TPIAYMTHVRTELGVKPAPIMARFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTG 540
Query: 541 SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKANDLNPI 600
SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKS IMTTAR KANDLN I
Sbjct: 541 SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSTIMTTARIKANDLNSI 600
Query: 601 LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF 660
LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF
Sbjct: 601 LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF 660
Query: 661 VCSKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPRVSISVEPNTL 720
VCSKKFKV DLNYPSITIDNLASEVVKIKRRVKN+G P+TYVANVKAPPRVS+SVEPNTL
Sbjct: 661 VCSKKFKVADLNYPSITIDNLASEVVKIKRRVKNVGSPATYVANVKAPPRVSVSVEPNTL 720
Query: 721 KFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVLG 769
KFTRTGEEKSFTVVLKRVPNDYHRG+VFGRLAWSNGKHHVRSPILVVLG
Sbjct: 721 KFTRTGEEKSFTVVLKRVPNDYHRGSVFGRLAWSNGKHHVRSPILVVLG 769
BLAST of Csor.00g030610 vs. NCBI nr
Match:
XP_023553317.1 (subtilisin-like protease SBT5.4 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1360 bits (3520), Expect = 0.0
Identity = 674/769 (87.65%), Postives = 721/769 (93.76%), Query Frame = 0
Query: 1 MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
MEAFNLPPLLLSFFLF+LLQTSTIA KKSYIVYLGSHSHGLNPSA+DLQLATQTHYNLLG
Sbjct: 1 MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWS 120
SVLGSNEAAKEAIFYSYNRHINGFAAVLD KVAEDVAKHP VISVHEN+GR+LHTTNSWS
Sbjct: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDQKVAEDVAKHPDVISVHENRGRKLHTTNSWS 120
Query: 121 FLGVENNGELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGG 180
FLGVEN+ +P NS+WNLA+FGESTIIGNLDTG+WPESKSFSDEGYGPIPKRWKGSCEGG
Sbjct: 121 FLGVENHDGIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPKRWKGSCEGG 180
Query: 181 SNFSCNRKLIGARYFNKGYASIVGPLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFGN 240
SNFSCNRKLIGARYFNKGYAS+VGPLNS TARDDQGHGTHTLSTA G+FVS+ANIFGN
Sbjct: 181 SNFSCNRKLIGARYFNKGYASMVGPLNS---TARDDQGHGTHTLSTAAGNFVSKANIFGN 240
Query: 241 GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEF 300
GNGTAKGGSPKALVAAY+VCWP V +G CF+SD+LA FEAAISDGVDVLSISLGGSPLEF
Sbjct: 241 GNGTAKGGSPKALVAAYRVCWPPVALGECFESDILAGFEAAISDGVDVLSISLGGSPLEF 300
Query: 301 HDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALGD 360
HDDPIAI SFHAVKNGITVVCSAGNSGPSPG+VANVAPWLITVGASTTDRLFTTYVALGD
Sbjct: 301 HDDPIAIASFHAVKNGITVVCSAGNSGPSPGTVANVAPWLITVGASTTDRLFTTYVALGD 360
Query: 361 KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCLS 420
KRHIKG S+SNKILPVQKFYPLIRALDAKNNN S++DA+LCL GSLDPKKV+ KIVVC
Sbjct: 361 KRHIKGGSLSNKILPVQKFYPLIRALDAKNNNSSEMDALLCLGGSLDPKKVKGKIVVCHR 420
Query: 421 GDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYINSTK 480
G+ V+KGYV AQAGAV MIL +D+ DGD L DAHLLPAS+I+Y DGELVYQYINSTK
Sbjct: 421 GNNGRVDKGYVAAQAGAVAMILVDDEVDGDGLMADAHLLPASHISYDDGELVYQYINSTK 480
Query: 481 TPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTH 540
TPMAY+TRVRTE GVKPAP+M FSSRGPN IEPS+LKPDITAPGVNILAA++E TSPT
Sbjct: 481 TPMAYVTRVRTESGVKPAPIMATFSSRGPNTIEPSMLKPDITAPGVNILAAYSEATSPTG 540
Query: 541 SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKANDLNPI 600
+ FDKRRVPF ILSGTSMSCPHISGIVGLLKTLYPKWSPA+M+SA+MTTARTKAN+LNPI
Sbjct: 541 APFDKRRVPFIILSGTSMSCPHISGIVGLLKTLYPKWSPAIMRSALMTTARTKANNLNPI 600
Query: 601 LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF 660
SS KEKANSFAYGAGHVNPNKAANPGLVYDLS EDYLN+LCARGYNKTQMKLFSNDTSF
Sbjct: 601 RSSRKEKANSFAYGAGHVNPNKAANPGLVYDLSIEDYLNYLCARGYNKTQMKLFSNDTSF 660
Query: 661 VCSKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPRVSISVEPNTL 720
VCSKKFKVTDLNYPSITIDN+ S+VV+IKRRVKN+G PSTYVANVKAPP VS+SVEPNTL
Sbjct: 661 VCSKKFKVTDLNYPSITIDNMRSKVVRIKRRVKNVGSPSTYVANVKAPPGVSVSVEPNTL 720
Query: 721 KFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVLG 769
KFT+TGEEKSF+VVLKRVPNDY RGA+FGRLAWSNGKH VRSPILVVLG
Sbjct: 721 KFTKTGEEKSFSVVLKRVPNDYRRGAMFGRLAWSNGKHRVRSPILVVLG 766
BLAST of Csor.00g030610 vs. NCBI nr
Match:
XP_022932415.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])
HSP 1 Score: 1357 bits (3513), Expect = 0.0
Identity = 673/769 (87.52%), Postives = 719/769 (93.50%), Query Frame = 0
Query: 1 MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
MEAFNLPPLLLSFFLFALLQTSTIA KKSYIVYLGSHSHGLNPSA DLQLATQTHYNLLG
Sbjct: 1 MEAFNLPPLLLSFFLFALLQTSTIAAKKSYIVYLGSHSHGLNPSAADLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWS 120
SVLGSNEAAKEAIFYSYNRHINGFAAVLD KVAEDVAKHP VISVHEN+GR+LHTTNSWS
Sbjct: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDQKVAEDVAKHPDVISVHENRGRKLHTTNSWS 120
Query: 121 FLGVENNGELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGG 180
FLGVEN+ +P NS+WNLA+FGESTIIGNLDTG+WPESKSFSDEGYGPIPKRWKGSCEGG
Sbjct: 121 FLGVENHDGIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPKRWKGSCEGG 180
Query: 181 SNFSCNRKLIGARYFNKGYASIVGPLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFGN 240
SNFSCNRKLIGARYFNKGYAS+VGPLNS TARDDQGHGTHTLSTA G+FVS+ANIFGN
Sbjct: 181 SNFSCNRKLIGARYFNKGYASMVGPLNS---TARDDQGHGTHTLSTAAGNFVSKANIFGN 240
Query: 241 GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEF 300
GNGTAKGGSPKALVAAY+VCWP V +G CF+SD+LA FEAAISDGVDVLSISLGGSPLEF
Sbjct: 241 GNGTAKGGSPKALVAAYRVCWPPVALGECFESDILAGFEAAISDGVDVLSISLGGSPLEF 300
Query: 301 HDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALGD 360
HDDPIAI SFHAVKNGITVVCSAGNSGPSPG+VANVAPWLITVGASTTDRLFTTYVALGD
Sbjct: 301 HDDPIAIASFHAVKNGITVVCSAGNSGPSPGTVANVAPWLITVGASTTDRLFTTYVALGD 360
Query: 361 KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCLS 420
KRHIKGES+SNKILPVQKFYPLIRALDAKNNN SD+DA+LCL GSLDPKKV+ KIVVC
Sbjct: 361 KRHIKGESLSNKILPVQKFYPLIRALDAKNNNNSDMDAILCLGGSLDPKKVKGKIVVCRR 420
Query: 421 GDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYINSTK 480
G+ V+KGY+ AQAGAVGMIL +D+ DGD L DAHLLPAS+I++ DGELVYQYINSTK
Sbjct: 421 GNNGRVDKGYMAAQAGAVGMILVDDEVDGDGLMADAHLLPASHISFADGELVYQYINSTK 480
Query: 481 TPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTH 540
TPMAY+T VRTE GVKPAP+M FSSRGPN IEPS+LKPDITAPGVNILAA++E TSPT
Sbjct: 481 TPMAYVTHVRTETGVKPAPIMATFSSRGPNTIEPSMLKPDITAPGVNILAAYSEATSPTG 540
Query: 541 SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKANDLNPI 600
+ FDKRRVPF ILSGTSMSCPHISGIVGLLKTLYPKWSPA+M+SAIMTTARTKAN+LNPI
Sbjct: 541 APFDKRRVPFIILSGTSMSCPHISGIVGLLKTLYPKWSPAIMRSAIMTTARTKANNLNPI 600
Query: 601 LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF 660
SS KEKANSFAYGAGHVNPN AANPGLVYDLS EDYLN+LCARGYNKTQ+KLFSNDTSF
Sbjct: 601 RSSRKEKANSFAYGAGHVNPNNAANPGLVYDLSIEDYLNYLCARGYNKTQIKLFSNDTSF 660
Query: 661 VCSKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPRVSISVEPNTL 720
VCSKKFKVTDLNYPSITIDN+ S+VV+IKRR+KN+G PSTYVANVKAP VSISVEPNTL
Sbjct: 661 VCSKKFKVTDLNYPSITIDNMRSKVVRIKRRLKNVGSPSTYVANVKAPSGVSISVEPNTL 720
Query: 721 KFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVLG 769
KFT+TGEEKSF+VVLKRVPNDY RGA+FGRLAWSNGKH VRSPILVVLG
Sbjct: 721 KFTKTGEEKSFSVVLKRVPNDYRRGAMFGRLAWSNGKHRVRSPILVVLG 766
BLAST of Csor.00g030610 vs. ExPASy TrEMBL
Match:
A0A6J1L3R2 (subtilisin-like protease SBT5.4 OS=Cucurbita maxima OX=3661 GN=LOC111500241 PE=3 SV=1)
HSP 1 Score: 1471 bits (3807), Expect = 0.0
Identity = 726/769 (94.41%), Postives = 749/769 (97.40%), Query Frame = 0
Query: 1 MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSA+DLQLATQTHYNLLG
Sbjct: 1 MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAVDLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWS 120
SV+GSNEAAKEAIFYSYNRHINGFAAVLD+KVAEDVAKHPSV+SVHENKGRELHTTNSWS
Sbjct: 61 SVIGSNEAAKEAIFYSYNRHINGFAAVLDYKVAEDVAKHPSVVSVHENKGRELHTTNSWS 120
Query: 121 FLGVENNGELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGG 180
FLGVENNG LP NSLWNLANFGESTIIGNLDTGIWPESKSF+DEGYGPIPKRWKGSCEGG
Sbjct: 121 FLGVENNGGLPPNSLWNLANFGESTIIGNLDTGIWPESKSFNDEGYGPIPKRWKGSCEGG 180
Query: 181 SNFSCNRKLIGARYFNKGYASIVGPLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFGN 240
SNF+CNRKLIGARYFNKGYASIVGPLNSSY TARDDQGHGTHTLSTAGGHFVSRANIFGN
Sbjct: 181 SNFNCNRKLIGARYFNKGYASIVGPLNSSYLTARDDQGHGTHTLSTAGGHFVSRANIFGN 240
Query: 241 GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEF 300
GNGTAKGGSPKALVAAYKVCWPRV +GGCFDSD+LA FEAAISDGVDVLSISLGGSPLEF
Sbjct: 241 GNGTAKGGSPKALVAAYKVCWPRVNIGGCFDSDILAGFEAAISDGVDVLSISLGGSPLEF 300
Query: 301 HDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALGD 360
HDDPIAI SFHAVKNGITVVCSAGNSGPSPG+VANVAPWLITVGAST DRLFTTY+ALGD
Sbjct: 301 HDDPIAIASFHAVKNGITVVCSAGNSGPSPGTVANVAPWLITVGASTIDRLFTTYLALGD 360
Query: 361 KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCLS 420
KRHIKGESISNKILPVQKFYPLIRALDAKNNNVS+I+A+LCLEGSLDPKKVE KIVVCLS
Sbjct: 361 KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSNIEAILCLEGSLDPKKVEGKIVVCLS 420
Query: 421 GDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYINSTK 480
GDI NV+KGYV AQAGAVGMIL NDKEDGDRLEV+AHLLP S+INYIDGELVYQ+INSTK
Sbjct: 421 GDIANVDKGYVAAQAGAVGMILTNDKEDGDRLEVEAHLLPTSHINYIDGELVYQFINSTK 480
Query: 481 TPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTH 540
TP+AYMT VRTELGVKPAP+M RFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPT
Sbjct: 481 TPIAYMTHVRTELGVKPAPIMARFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTG 540
Query: 541 SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKANDLNPI 600
SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKS IMTTAR KANDLN I
Sbjct: 541 SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSTIMTTARIKANDLNSI 600
Query: 601 LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF 660
LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF
Sbjct: 601 LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF 660
Query: 661 VCSKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPRVSISVEPNTL 720
VCSKKFKV DLNYPSITIDNLASEVVKIKRRVKN+G P+TYVANVKAPPRVS+SVEPNTL
Sbjct: 661 VCSKKFKVADLNYPSITIDNLASEVVKIKRRVKNVGSPATYVANVKAPPRVSVSVEPNTL 720
Query: 721 KFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVLG 769
KFTRTGEEKSFTVVLKRVPNDYHRG+VFGRLAWSNGKHHVRSPILVVLG
Sbjct: 721 KFTRTGEEKSFTVVLKRVPNDYHRGSVFGRLAWSNGKHHVRSPILVVLG 769
BLAST of Csor.00g030610 vs. ExPASy TrEMBL
Match:
A0A6J1F248 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111438770 PE=3 SV=1)
HSP 1 Score: 1357 bits (3513), Expect = 0.0
Identity = 673/769 (87.52%), Postives = 719/769 (93.50%), Query Frame = 0
Query: 1 MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
MEAFNLPPLLLSFFLFALLQTSTIA KKSYIVYLGSHSHGLNPSA DLQLATQTHYNLLG
Sbjct: 1 MEAFNLPPLLLSFFLFALLQTSTIAAKKSYIVYLGSHSHGLNPSAADLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWS 120
SVLGSNEAAKEAIFYSYNRHINGFAAVLD KVAEDVAKHP VISVHEN+GR+LHTTNSWS
Sbjct: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDQKVAEDVAKHPDVISVHENRGRKLHTTNSWS 120
Query: 121 FLGVENNGELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGG 180
FLGVEN+ +P NS+WNLA+FGESTIIGNLDTG+WPESKSFSDEGYGPIPKRWKGSCEGG
Sbjct: 121 FLGVENHDGIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPKRWKGSCEGG 180
Query: 181 SNFSCNRKLIGARYFNKGYASIVGPLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFGN 240
SNFSCNRKLIGARYFNKGYAS+VGPLNS TARDDQGHGTHTLSTA G+FVS+ANIFGN
Sbjct: 181 SNFSCNRKLIGARYFNKGYASMVGPLNS---TARDDQGHGTHTLSTAAGNFVSKANIFGN 240
Query: 241 GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEF 300
GNGTAKGGSPKALVAAY+VCWP V +G CF+SD+LA FEAAISDGVDVLSISLGGSPLEF
Sbjct: 241 GNGTAKGGSPKALVAAYRVCWPPVALGECFESDILAGFEAAISDGVDVLSISLGGSPLEF 300
Query: 301 HDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALGD 360
HDDPIAI SFHAVKNGITVVCSAGNSGPSPG+VANVAPWLITVGASTTDRLFTTYVALGD
Sbjct: 301 HDDPIAIASFHAVKNGITVVCSAGNSGPSPGTVANVAPWLITVGASTTDRLFTTYVALGD 360
Query: 361 KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCLS 420
KRHIKGES+SNKILPVQKFYPLIRALDAKNNN SD+DA+LCL GSLDPKKV+ KIVVC
Sbjct: 361 KRHIKGESLSNKILPVQKFYPLIRALDAKNNNNSDMDAILCLGGSLDPKKVKGKIVVCRR 420
Query: 421 GDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYINSTK 480
G+ V+KGY+ AQAGAVGMIL +D+ DGD L DAHLLPAS+I++ DGELVYQYINSTK
Sbjct: 421 GNNGRVDKGYMAAQAGAVGMILVDDEVDGDGLMADAHLLPASHISFADGELVYQYINSTK 480
Query: 481 TPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTH 540
TPMAY+T VRTE GVKPAP+M FSSRGPN IEPS+LKPDITAPGVNILAA++E TSPT
Sbjct: 481 TPMAYVTHVRTETGVKPAPIMATFSSRGPNTIEPSMLKPDITAPGVNILAAYSEATSPTG 540
Query: 541 SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKANDLNPI 600
+ FDKRRVPF ILSGTSMSCPHISGIVGLLKTLYPKWSPA+M+SAIMTTARTKAN+LNPI
Sbjct: 541 APFDKRRVPFIILSGTSMSCPHISGIVGLLKTLYPKWSPAIMRSAIMTTARTKANNLNPI 600
Query: 601 LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF 660
SS KEKANSFAYGAGHVNPN AANPGLVYDLS EDYLN+LCARGYNKTQ+KLFSNDTSF
Sbjct: 601 RSSRKEKANSFAYGAGHVNPNNAANPGLVYDLSIEDYLNYLCARGYNKTQIKLFSNDTSF 660
Query: 661 VCSKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPRVSISVEPNTL 720
VCSKKFKVTDLNYPSITIDN+ S+VV+IKRR+KN+G PSTYVANVKAP VSISVEPNTL
Sbjct: 661 VCSKKFKVTDLNYPSITIDNMRSKVVRIKRRLKNVGSPSTYVANVKAPSGVSISVEPNTL 720
Query: 721 KFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVLG 769
KFT+TGEEKSF+VVLKRVPNDY RGA+FGRLAWSNGKH VRSPILVVLG
Sbjct: 721 KFTKTGEEKSFSVVLKRVPNDYRRGAMFGRLAWSNGKHRVRSPILVVLG 766
BLAST of Csor.00g030610 vs. ExPASy TrEMBL
Match:
A0A6J1EW82 (subtilisin-like protease SBT5.3 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111438753 PE=3 SV=1)
HSP 1 Score: 1241 bits (3210), Expect = 0.0
Identity = 612/768 (79.69%), Postives = 676/768 (88.02%), Query Frame = 0
Query: 1 MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
MEAFNLPPLLLSFFLFALLQTSTIA KKSYIVYLGSHSHGLNPSA+DLQLATQTHYNLLG
Sbjct: 1 MEAFNLPPLLLSFFLFALLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWS 120
SVLGSNEAAKEAIFYSYNRHINGFAAVLD KVAEDVAK P V+SV+ENKG +LHTT SW+
Sbjct: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDQKVAEDVAKRPDVVSVYENKGLKLHTTRSWN 120
Query: 121 FLGVENNGELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGG 180
FLGVEN+G +P NSLWNL+ FGESTIIGNLDTG+WPESKSFSD+ YGPIP RWKGSCEGG
Sbjct: 121 FLGVENDGGIPSNSLWNLSRFGESTIIGNLDTGVWPESKSFSDKEYGPIPTRWKGSCEGG 180
Query: 181 SNFSCNRKLIGARYFNKGYASIVGPLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFGN 240
SNF+CNRKLIGARYFNKGYAS+ GPLNSSY TARDD GHGTHTLSTAGG+FV +IFGN
Sbjct: 181 SNFNCNRKLIGARYFNKGYASVAGPLNSSYETARDDNGHGTHTLSTAGGNFVEGVSIFGN 240
Query: 241 GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEF 300
G GTAKGGSPK+LVAAYKVCWP V+ GGCF +D+LA FEAAISDGVDVLS+SLGG EF
Sbjct: 241 GYGTAKGGSPKSLVAAYKVCWPPVEDGGCFVADILAGFEAAISDGVDVLSVSLGGRVQEF 300
Query: 301 HDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALGD 360
+DD +AIGSFHAVKNGITVVCSAGNSGP G+ +NVAPW++TVGAST DRLFTT V LGD
Sbjct: 301 YDDILAIGSFHAVKNGITVVCSAGNSGPYEGTASNVAPWMLTVGASTIDRLFTTNVTLGD 360
Query: 361 KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCLS 420
KRH KGES+S KILP +KFYPLIRALDAK NNVSD + LCLEG+LDPKKVE KIVVCL
Sbjct: 361 KRHFKGESLSGKILPAKKFYPLIRALDAKFNNVSDYETGLCLEGTLDPKKVEGKIVVCLR 420
Query: 421 GDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYINSTK 480
GD + V+KGYVVAQAG VGMILAND G L +AH+LPAS+I+Y DGE VY+YI STK
Sbjct: 421 GDNSRVDKGYVVAQAGGVGMILANDLLGGRALLSEAHILPASHISYTDGESVYKYIQSTK 480
Query: 481 TPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTH 540
TPMAYMT VRTELG+KPAP+M FSSRGPN+IEP+ILKPDITAPGVNILAAF+ PTH
Sbjct: 481 TPMAYMTSVRTELGIKPAPIMASFSSRGPNVIEPTILKPDITAPGVNILAAFSNIAPPTH 540
Query: 541 SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKANDLNPI 600
S FDKRRV +N+LSGTSMSCPHISGIVGLLKTLYPKWSPA ++SAIMTTA TKANDLNPI
Sbjct: 541 SHFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETKANDLNPI 600
Query: 601 LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF 660
L+S +EKA FAYGAGHV PNKAANPGLVYDLST+DYLNFLCARGYNKTQM+LF+NDTSF
Sbjct: 601 LTSIEEKAGPFAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKTQMRLFTNDTSF 660
Query: 661 VCSKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPRVSISVEPNTL 720
VCSK FKVTDLNYPSI+I +L S+ V IKRRVKN+G P TYVA V+APP + +SV+P+TL
Sbjct: 661 VCSKSFKVTDLNYPSISIFDLRSKEVNIKRRVKNVGSPGTYVAKVEAPPGILVSVDPSTL 720
Query: 721 KFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVL 768
KFT++ EEK F VVLKRVPN+ VFG+L WS+GKH V SPI V L
Sbjct: 721 KFTKSDEEKDFKVVLKRVPNNQTENHVFGKLVWSDGKHRVSSPISVTL 768
BLAST of Csor.00g030610 vs. ExPASy TrEMBL
Match:
A0A6J1F1I2 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111438753 PE=3 SV=1)
HSP 1 Score: 1236 bits (3197), Expect = 0.0
Identity = 609/768 (79.30%), Postives = 674/768 (87.76%), Query Frame = 0
Query: 1 MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
MEAFNLPPLLLSFFLF LLQTSTIA KKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG
Sbjct: 1 MEAFNLPPLLLSFFLFVLLQTSTIAAKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWS 120
S+LGSNEAAKEAIFYSYNRHIN FAAVLD KVAED+AK P V+SV+ENKG +LHTT SW+
Sbjct: 61 SMLGSNEAAKEAIFYSYNRHINAFAAVLDQKVAEDIAKRPDVVSVYENKGLKLHTTRSWN 120
Query: 121 FLGVENNGELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGG 180
FLGVEN+G +P NSLWNL+ FGESTIIGNLDTG+WPESKSFSD+ YGPIP RWKGSCEGG
Sbjct: 121 FLGVENDGGIPSNSLWNLSRFGESTIIGNLDTGVWPESKSFSDKEYGPIPTRWKGSCEGG 180
Query: 181 SNFSCNRKLIGARYFNKGYASIVGPLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFGN 240
SNF+CNRKLIGARYFNKGYAS+ GPLNSSY TARDD GHGTHTLSTAGG+FV +IFGN
Sbjct: 181 SNFNCNRKLIGARYFNKGYASVAGPLNSSYETARDDNGHGTHTLSTAGGNFVEGVSIFGN 240
Query: 241 GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEF 300
G GTAKGGSPK+LVAAYKVCWP V+ GGCF +D+LA FEAAISDGVDVLS+SLGG EF
Sbjct: 241 GYGTAKGGSPKSLVAAYKVCWPPVEDGGCFVADILAGFEAAISDGVDVLSVSLGGRVQEF 300
Query: 301 HDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALGD 360
+DD +AIGSFHAVKNGITVVCSAGNSGP G+ +NVAPW++TVGAST DRLFTT V LGD
Sbjct: 301 YDDILAIGSFHAVKNGITVVCSAGNSGPYEGTASNVAPWMLTVGASTIDRLFTTNVTLGD 360
Query: 361 KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCLS 420
KRH KGES+S KILP +KFYPLIRALDAK NNVSD + LCLEG+LDPKKVE KIVVCL
Sbjct: 361 KRHFKGESLSGKILPAKKFYPLIRALDAKFNNVSDYETGLCLEGTLDPKKVEGKIVVCLR 420
Query: 421 GDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYINSTK 480
GD + V+KGYVVAQAG VGMILAND G L +AH+LPAS+I+Y DGE VY+YI STK
Sbjct: 421 GDNSRVDKGYVVAQAGGVGMILANDLLGGRALLSEAHILPASHISYTDGESVYKYIQSTK 480
Query: 481 TPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTH 540
TPMAYMT VRTELG+KPAP+M FSSRGPN+IEP+ILKPDITAPGVNILAAF+ PTH
Sbjct: 481 TPMAYMTSVRTELGIKPAPIMASFSSRGPNVIEPTILKPDITAPGVNILAAFSNIAPPTH 540
Query: 541 SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKANDLNPI 600
S FDKRRV +N+LSGTSMSCPHISGIVGLLKTLYPKWSPA ++SAIMTTA TKANDLNPI
Sbjct: 541 SHFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETKANDLNPI 600
Query: 601 LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF 660
L+S +EKA FAYGAGHV PNKAANPGLVYDLST+DYLNFLCARGYNKTQM+LF+NDTSF
Sbjct: 601 LTSIEEKAGPFAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKTQMRLFTNDTSF 660
Query: 661 VCSKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPRVSISVEPNTL 720
VCSK FKVTDLNYPSI+I +L S+ V IKRRVKN+G P TYVA V+APP + +SV+P+TL
Sbjct: 661 VCSKSFKVTDLNYPSISIFDLRSKEVNIKRRVKNVGSPGTYVAKVEAPPGILVSVDPSTL 720
Query: 721 KFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVL 768
KFT++ EEK F VVLKRVPN+ VFG+L WS+GKH V SPI V L
Sbjct: 721 KFTKSDEEKDFKVVLKRVPNNQTENHVFGKLVWSDGKHRVSSPISVTL 768
BLAST of Csor.00g030610 vs. ExPASy TrEMBL
Match:
A0A6J1EWV1 (subtilisin-like protease SBT5.3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111438753 PE=3 SV=1)
HSP 1 Score: 1233 bits (3191), Expect = 0.0
Identity = 608/768 (79.17%), Postives = 673/768 (87.63%), Query Frame = 0
Query: 1 MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
MEAFNLPPLL+SFFL LLQTSTIA KKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG
Sbjct: 1 MEAFNLPPLLVSFFLLVLLQTSTIAAKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
Query: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWS 120
SVLGSNEAAKEAIFYSYNRHIN FAAVLD KVAED+AK P V+SV+ENKG +LHTT SW+
Sbjct: 61 SVLGSNEAAKEAIFYSYNRHINAFAAVLDQKVAEDIAKRPDVVSVYENKGLKLHTTRSWN 120
Query: 121 FLGVENNGELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGG 180
FLGVEN+G +P NSLWNL+ FGESTIIGNLDTG+WPESKSFSD+ YGPIP RWKGSCEGG
Sbjct: 121 FLGVENDGGIPSNSLWNLSRFGESTIIGNLDTGVWPESKSFSDKEYGPIPTRWKGSCEGG 180
Query: 181 SNFSCNRKLIGARYFNKGYASIVGPLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFGN 240
SNF+CNRKLIGARYFNKGYAS+ GPLNSSY TARDD GHGTHTLSTAGG+FV +IFGN
Sbjct: 181 SNFNCNRKLIGARYFNKGYASVAGPLNSSYETARDDNGHGTHTLSTAGGNFVEGVSIFGN 240
Query: 241 GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEF 300
G GTAKGGSPK+LVAAYKVCWP V+ GGCF +D+LA FEAAISDGVDVLS+SLGG EF
Sbjct: 241 GYGTAKGGSPKSLVAAYKVCWPPVEDGGCFVADILAGFEAAISDGVDVLSVSLGGRVQEF 300
Query: 301 HDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALGD 360
+DD +AIGSFHAVKNGITVVCSAGNSGP G+ +NVAPW++TVGAST DRLFTT V LGD
Sbjct: 301 YDDILAIGSFHAVKNGITVVCSAGNSGPYEGTASNVAPWMLTVGASTIDRLFTTNVTLGD 360
Query: 361 KRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCLS 420
KRH KGES+S KILP +KFYPLIRALDAK NNVSD + LCLEG+LDPKKVE KIVVCL
Sbjct: 361 KRHFKGESLSGKILPAKKFYPLIRALDAKFNNVSDYETGLCLEGTLDPKKVEGKIVVCLR 420
Query: 421 GDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYINSTK 480
GD + V+KGYVVAQAG VGMILAND G L +AH+LPAS+I+Y DGE VY+YI STK
Sbjct: 421 GDNSRVDKGYVVAQAGGVGMILANDLLGGRALLSEAHILPASHISYTDGESVYKYIQSTK 480
Query: 481 TPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPTH 540
TPMAYMT VRTELG+KPAP+M FSSRGPN+IEP+ILKPDITAPGVNILAAF+ PTH
Sbjct: 481 TPMAYMTSVRTELGIKPAPIMASFSSRGPNVIEPTILKPDITAPGVNILAAFSNIAPPTH 540
Query: 541 SSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKANDLNPI 600
S FDKRRV +N+LSGTSMSCPHISGIVGLLKTLYPKWSPA ++SAIMTTA TKANDLNPI
Sbjct: 541 SHFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETKANDLNPI 600
Query: 601 LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSF 660
L+S +EKA FAYGAGHV PNKAANPGLVYDLST+DYLNFLCARGYNKTQM+LF+NDTSF
Sbjct: 601 LTSIEEKAGPFAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKTQMRLFTNDTSF 660
Query: 661 VCSKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPRVSISVEPNTL 720
VCSK FKVTDLNYPSI+I +L S+ V IKRRVKN+G P TYVA V+APP + +SV+P+TL
Sbjct: 661 VCSKSFKVTDLNYPSISIFDLRSKEVNIKRRVKNVGSPGTYVAKVEAPPGILVSVDPSTL 720
Query: 721 KFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVL 768
KFT++ EEK F VVLKRVPN+ VFG+L WS+GKH V SPI V L
Sbjct: 721 KFTKSDEEKDFKVVLKRVPNNQTENHVFGKLVWSDGKHRVSSPISVTL 768
BLAST of Csor.00g030610 vs. TAIR 10
Match:
AT5G59810.1 (Subtilase family protein )
HSP 1 Score: 875.9 bits (2262), Expect = 2.3e-254
Identity = 450/769 (58.52%), Postives = 547/769 (71.13%), Query Frame = 0
Query: 1 MEAFNLPPLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60
M +L LLL L L + A+KKSYIVYLGSH+H S+ L +H L
Sbjct: 16 MSLQSLSSLLL---LVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLA 75
Query: 61 SVLGSNEAAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWS 120
S +GS+E AKEAIFYSY RHINGFAA+LD A ++AKHP V+SV NKGR+LHTT+SW+
Sbjct: 76 SFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWN 135
Query: 121 FLGVENNGELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGG 180
F+ + NG + +SLWN A +GE TII NLDTG+WPESKSFSDEGYG +P RWKG C
Sbjct: 136 FMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH-- 195
Query: 181 SNFSCNRKLIGARYFNKGYASIVG-PLNSSYATARDDQGHGTHTLSTAGGHFVSRANIFG 240
+ CNRKLIGARYFNKGY + G P N+SY T RD GHG+HTLSTA G+FV AN+FG
Sbjct: 196 KDVPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFG 255
Query: 241 NGNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLE 300
GNGTA GGSPKA VAAYKVCWP V CFD+D+LAA EAAI DGVDVLS S+GG +
Sbjct: 256 IGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGD 315
Query: 301 FHDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALG 360
+ D IAIGSFHAVKNG+TVVCSAGNSGP G+V+NVAPW+ITVGAS+ DR F +V L
Sbjct: 316 YMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELK 375
Query: 361 DKRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCL 420
+ + KG S+S K LP +K Y LI A DA N + DA+LC +GSLDPKKV+ KI+VCL
Sbjct: 376 NGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCL 435
Query: 421 SGDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYINST 480
GD V+KG A AGA GM+L NDK G+ + DAH+LPAS I+Y DGE ++ Y++ST
Sbjct: 436 RGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSST 495
Query: 481 KTPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSPT 540
K P Y+ L KPAP M FSSRGPN I P ILKPDITAPGVNI+AAFTE T PT
Sbjct: 496 KDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPT 555
Query: 541 HSSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKANDLNP 600
D RR PFN SGTSMSCPHISG+VGLLKTL+P WSPA ++SAIMTT+RT+ N P
Sbjct: 556 DLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKP 615
Query: 601 ILSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTS 660
++ +KAN F+YG+GHV PNKAA+PGLVYDL+T DYL+FLCA GYN T ++LF+ D
Sbjct: 616 MVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQ 675
Query: 661 FVCSKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPRVSISVEPNT 720
+ C + + D NYPSIT+ NL + + R++KN+G P+TY A + P V +SVEP
Sbjct: 676 YTCRQGANLLDFNYPSITVPNLTGSIT-VTRKLKNVGPPATYNARFREPLGVRVSVEPKQ 735
Query: 721 LKFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILVVL 769
L F +TGE K F + L+ +P G VFG L W++ H+VRSPI+V L
Sbjct: 736 LTFNKTGEVKIFQMTLRPLPVT-PSGYVFGELTWTDSHHYVRSPIVVQL 776
BLAST of Csor.00g030610 vs. TAIR 10
Match:
AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 857.8 bits (2215), Expect = 6.5e-249
Identity = 424/742 (57.14%), Postives = 531/742 (71.56%), Query Frame = 0
Query: 29 SYIVYLGSHSHGLNPSAIDLQLATQTHYNLLGSVLGSNEAAKEAIFYSYNRHINGFAAVL 88
SY+VY G+HSH + + +THY+ LGS GS E A +AIFYSY +HINGFAA L
Sbjct: 31 SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90
Query: 89 DHKVAEDVAKHPSVISVHENKGRELHTTNSWSFLGVENNGELPLNSLWNLANFGESTIIG 148
DH +A +++KHP V+SV NK +LHTT SW FLG+E+N +P +S+W A FGE TII
Sbjct: 91 DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150
Query: 149 NLDTGIWPESKSFSDEGYGPIPKRWKGSCEG--GSNFSCNRKLIGARYFNKGYASIVGPL 208
NLDTG+WPESKSF DEG GPIP RWKG C+ + F CNRKLIGARYFNKGYA+ VG L
Sbjct: 151 NLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHL 210
Query: 209 NSSYATARDDQGHGTHTLSTAGGHFVSRANIFGNGNGTAKGGSPKALVAAYKVCWPRVKV 268
NSS+ + RD GHG+HTLSTA G FV +IFG GNGTAKGGSP+A VAAYKVCWP VK
Sbjct: 211 NSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKG 270
Query: 269 GGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEFHDDPIAIGSFHAVKNGITVVCSAGNS 328
C+D+DVLAAF+AAI DG DV+S+SLGG P F +D +AIGSFHA K I VVCSAGNS
Sbjct: 271 NECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNS 330
Query: 329 GPSPGSVANVAPWLITVGASTTDRLFTTYVALGDKRHIKGESISNKILPVQKFYPLIRAL 388
GP+ +V+NVAPW ITVGAST DR F + + LG+ +H KG+S+S+ LP KFYP++ ++
Sbjct: 331 GPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASV 390
Query: 389 DAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVCLSGDITNVEKGYVVAQAGAVGMILANDK 448
+AK N S +DA LC GSLDP K + KI+VCL G VEKG VA G +GM+L N
Sbjct: 391 NAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTY 450
Query: 449 EDGDRLEVDAHLLPASNINYIDGELVYQYINSTKTPMAYMTRVRTELGVKPAPVMGRFSS 508
G+ L D H+LPA+ + D V +YI+ TK P+A++T RT+LG+KPAPVM FSS
Sbjct: 451 VTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSS 510
Query: 509 RGPNIIEPSILKPDITAPGVNILAAFTEETSPTHSSFDKRRVPFNILSGTSMSCPHISGI 568
+GP+I+ P ILKPDITAPGV+++AA+T SPT+ FD RR+ FN +SGTSMSCPHISGI
Sbjct: 511 KGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGI 570
Query: 569 VGLLKTLYPKWSPAVMKSAIMTTARTKANDLNPILSSGKEKANSFAYGAGHVNPNKAANP 628
GLLKT YP WSPA ++SAIMTTA + PI ++ KA F++GAGHV PN A NP
Sbjct: 571 AGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNP 630
Query: 629 GLVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSFVCSKKFKVTDLNYPSITIDNLASEVV 688
GLVYDL +DYLNFLC+ GYN +Q+ +FS + S K + +LNYPSIT+ NL S V
Sbjct: 631 GLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKV 690
Query: 689 KIKRRVKNLGCPSTYVANVKAPPRVSISVEPNTLKFTRTGEEKSFTVVLKRVPNDYHRGA 748
+ R VKN+G PS Y V P V ++V+P +L FT+ GE+K+F V+L + + +G
Sbjct: 691 TVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGY 750
Query: 749 VFGRLAWSNGKHHVRSPILVVL 769
VFG L WS+ KH VRSPI+V L
Sbjct: 751 VFGELVWSDKKHRVRSPIVVKL 772
BLAST of Csor.00g030610 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 610.9 bits (1574), Expect = 1.4e-174
Identity = 351/774 (45.35%), Postives = 476/774 (61.50%), Query Frame = 0
Query: 12 SFFLFALL----QTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLGSVLGSNE 71
+FFL L +S+ + + +YIV++ PS+ DL H N S L S
Sbjct: 10 AFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQ---MPSSFDL------HSNWYDSSLRSIS 69
Query: 72 AAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWSFLGVENN 131
+ E + Y+Y I+GF+ L + A+ + P VISV ELHTT + FLG++ +
Sbjct: 70 DSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH 129
Query: 132 GELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGGSNFS--- 191
L+ A ++G LDTG+WPESKS+SDEG+GPIP WKG CE G+NF+
Sbjct: 130 ----TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASL 189
Query: 192 CNRKLIGARYFNKGYASIVGPLNSS--YATARDDQGHGTHTLSTAGGHFVSRANIFGNGN 251
CNRKLIGAR+F +GY S +GP++ S + RDD GHGTHT STA G V A++ G +
Sbjct: 190 CNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYAS 249
Query: 252 GTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGSPLEFHD 311
GTA+G +P+A VA YKVCW +GGCF SD+LAA + AI+D V+VLS+SLGG +++
Sbjct: 250 GTARGMAPRARVAVYKVCW----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYR 309
Query: 312 DPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVALGDKR 371
D +AIG+F A++ GI V CSAGN+GPS S++NVAPW+ TVGA T DR F LG+ +
Sbjct: 310 DGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGK 369
Query: 372 H------IKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIV 431
+ KGE++ +K+LP F A +A N N LC+ G+L P+KV+ KIV
Sbjct: 370 NFTGVSLFKGEALPDKLLP---FIYAGNASNATNGN-------LCMTGTLIPEKVKGKIV 429
Query: 432 VCLSGDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYI 491
+C G V+KG VV AG VGMILAN +G+ L DAHLLPA+ + G+++ Y+
Sbjct: 430 MCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYV 489
Query: 492 NSTKTPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEET 551
+ P A ++ + T +GVKP+PV+ FSSRGPN I P+ILKPD+ APGVNILAA+T
Sbjct: 490 TTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAA 549
Query: 552 SPTHSSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKAND 611
PT + D RRV FNI+SGTSMSCPH+SG+ LLK+++P+WSPA ++SA+MTTA D
Sbjct: 550 GPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKD 609
Query: 612 LNPILSSGKEKANS-FAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMKLFS 671
P+L K ++ F +GAGHV+P A NPGL+YDL+TEDYL FLCA Y Q++ S
Sbjct: 610 GKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVS 669
Query: 672 NDTSFVC--SKKFKVTDLNYPSITIDNLASEVVKIKRRVKNLGCPSTYVANVKAPPR-VS 731
++ C SK + V DLNYPS ++ K R V ++G TY V + V
Sbjct: 670 R-RNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVK 729
Query: 732 ISVEPNTLKFTRTGEEKSFTVVLKRVPNDYHRGAVFGRLAWSNGKHHVRSPILV 767
ISVEP L F E+KS+TV + FG + WS+GKH V SP+ +
Sbjct: 730 ISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754
BLAST of Csor.00g030610 vs. TAIR 10
Match:
AT3G14067.1 (Subtilase family protein )
HSP 1 Score: 580.1 bits (1494), Expect = 2.6e-165
Identity = 351/788 (44.54%), Postives = 473/788 (60.03%), Query Frame = 0
Query: 8 PLLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLGSVLGSNE 67
PLLL FF +S+ +SYIV++ SH PS H +LL S+ S +
Sbjct: 14 PLLLCFF---SPSSSSSDGLESYIVHV-QRSH--KPSLFSSH--NNWHVSLLRSLPSSPQ 73
Query: 68 AAKEAIFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWSFLGVENN 127
A + YSY+R ++GF+A L + +HPSVISV ++ RE+HTT++ +FLG N
Sbjct: 74 PA--TLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQN 133
Query: 128 GELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGGSNF---S 187
+ LW+ +N+GE I+G LDTGIWPE SFSD G GPIP WKG CE G +F S
Sbjct: 134 -----SGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASS 193
Query: 188 CNRKLIGARYFNKGYASIVGPLNSSYA----TARDDQGHGTHTLSTAGGHFVSRANIFGN 247
CNRKLIGAR F +GY + A + RD +GHGTHT STA G V+ A+++
Sbjct: 194 CNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQY 253
Query: 248 GNGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLG--GSPL 307
GTA G + KA +AAYK+CW GGC+DSD+LAA + A++DGV V+S+S+G GS
Sbjct: 254 ARGTATGMASKARIAAYKICW----TGGCYDSDILAAMDQAVADGVHVISLSVGASGSAP 313
Query: 308 EFHDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVAL 367
E+H D IAIG+F A ++GI V CSAGNSGP+P + N+APW++TVGAST DR F
Sbjct: 314 EYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAIT 373
Query: 368 GDKRHIKGESI-SNKILPVQKFYPLIRALDAKNNNV--SDIDAVLCLEGSLDPKKVEEKI 427
GD + G S+ + + LP D++ + V D + LC G L+ VE KI
Sbjct: 374 GDGKVFTGTSLYAGESLP-----------DSQLSLVYSGDCGSRLCYPGKLNSSLVEGKI 433
Query: 428 VVCLSGDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQY 487
V+C G VEKG V AG GMILAN E G+ L D+HL+PA+ + G+ + Y
Sbjct: 434 VLCDRGGNARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDY 493
Query: 488 INSTKTPMAYMTRVRTELGVK-PAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTE 547
I ++ +P A ++ + T +G P+P + FSSRGPN + P ILKPD+ APGVNILA +T
Sbjct: 494 IKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTG 553
Query: 548 ETSPTHSSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKA 607
PT D RRV FNI+SGTSMSCPH+SG+ LL+ +P WSPA +KSA++TTA
Sbjct: 554 MVGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVE 613
Query: 608 NDLNPI--LSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYNKTQMK 667
N PI L++GK +NSF +GAGHV+PNKA NPGLVYD+ ++Y+ FLCA GY +
Sbjct: 614 NSGEPIEDLATGK-SSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGIL 673
Query: 668 LFSNDTSFV----CSKKFKVTDLNYPSITIDNLAS-EVVKIKRRVKNLG--CPSTYVANV 727
+F D + SK DLNYPS ++ ++ EVVK KR VKN+G + Y V
Sbjct: 674 VFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGV 733
Query: 728 KAPPRVSISVEPNTLKFTRTGEEKSFTVVLKRVPNDYHRGAV----FGRLAWSNGKHHVR 770
K+P V I V P+ L F++ + V K V G+V FG + W++G+H V+
Sbjct: 734 KSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVK 770
BLAST of Csor.00g030610 vs. TAIR 10
Match:
AT4G34980.1 (subtilisin-like serine protease 2 )
HSP 1 Score: 571.2 bits (1471), Expect = 1.2e-162
Identity = 342/780 (43.85%), Postives = 471/780 (60.38%), Query Frame = 0
Query: 9 LLLSFFLFALLQTSTIAVKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLGSVLGSNEA 68
+LL F F + + K++I + G PS THY+ S E
Sbjct: 7 VLLLFLSFPFISFAASQAAKTFIFRIDG---GSMPSIF------PTHYHWY-----STEF 66
Query: 69 AKEA-IFYSYNRHINGFAAVLDHKVAEDVAKHPSVISVHENKGRELHTTNSWSFLGVENN 128
A+E+ I + Y+ +GF+AV+ A+++ HP+V++V E++ RELHTT S FLG++N
Sbjct: 67 AEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQ 126
Query: 129 GELPLNSLWNLANFGESTIIGNLDTGIWPESKSFSDEGYGPIPKRWKGSCEGGSNFS--- 188
LW+ +++G IIG DTGIWPE +SFSD GPIPKRW+G CE G+ FS
Sbjct: 127 -----KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRN 186
Query: 189 CNRKLIGARYFNKG-YASIVGPLNSS--YATARDDQGHGTHTLSTAGGHFVSRANIFGNG 248
CNRK+IGAR+F KG A+++G +N + + + RD GHGTHT STA G +A++ G
Sbjct: 187 CNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYA 246
Query: 249 NGTAKGGSPKALVAAYKVCWPRVKVGGCFDSDVLAAFEAAISDGVDVLSISLGGS---PL 308
+G AKG +PKA +AAYKVCW K GC DSD+LAAF+AA+ DGVDV+SIS+GG
Sbjct: 247 SGVAKGVAPKARIAAYKVCW---KDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITS 306
Query: 309 EFHDDPIAIGSFHAVKNGITVVCSAGNSGPSPGSVANVAPWLITVGASTTDRLFTTYVAL 368
++ DPIAIGS+ A GI V SAGN GP+ SV N+APW+ TVGAST DR F L
Sbjct: 307 PYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAIL 366
Query: 369 GDKRHIKGESISNKILPVQKFYPLIRALDAKNNNVSDIDAVLCLEGSLDPKKVEEKIVVC 428
GD ++G S+ + + +P++ K+ S A LC+E +LDPK+V KIV+C
Sbjct: 367 GDGHRLRGVSLYAGVPLNGRMFPVV--YPGKSGMSS---ASLCMENTLDPKQVRGKIVIC 426
Query: 429 LSGDITNVEKGYVVAQAGAVGMILANDKEDGDRLEVDAHLLPASNINYIDGELVYQYINS 488
G V KG VV +AG VGMILAN +G+ L DAHL+PA + +G+ + Y +S
Sbjct: 427 DRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASS 486
Query: 489 TKTPMAYMTRVRTELGVKPAPVMGRFSSRGPNIIEPSILKPDITAPGVNILAAFTEETSP 548
P+A + T +G+KPAPV+ FS RGPN + P ILKPD+ APGVNILAA+T+ P
Sbjct: 487 HPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGP 546
Query: 549 THSSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSAIMTTARTKANDLN 608
T D R+ FNILSGTSM+CPH+SG LLK+ +P WSPAV++SA+MTT N
Sbjct: 547 TGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNR 606
Query: 609 PIL--SSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYN-KTQMKLFS 668
++ S+GK A + YG+GH+N +A NPGLVYD++ +DY+ FLC+ GY KT +
Sbjct: 607 SLIDESTGK-SATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITR 666
Query: 669 NDTSFVCSKKFKVTDLNYPSITI---DNLASEVVK-IKRRVKNLG-CPSTYVANVKAPPR 728
++K +LNYPSIT N V K + R N+G + Y A +++P
Sbjct: 667 TPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRG 726
Query: 729 VSISVEPNTLKFTRTGEEKSFTVVLKRVPNDY---HRGAVFGRLAW-SNGKHHVRSPILV 767
V+++V+P L FT + +S+ V + + GAVFG + W GKH VRSPI+V
Sbjct: 727 VTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVV 758
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| F4JXC5 | 3.3e-253 | 58.52 | Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... | [more] |
| Q9ZSP5 | 9.2e-248 | 57.14 | Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... | [more] |
| I1N462 | 2.9e-209 | 51.15 | Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... | [more] |
| O65351 | 2.0e-173 | 45.35 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| Q9LVJ1 | 3.7e-164 | 44.54 | Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1L3R2 | 0.0 | 94.41 | subtilisin-like protease SBT5.4 OS=Cucurbita maxima OX=3661 GN=LOC111500241 PE=3... | [more] |
| A0A6J1F248 | 0.0 | 87.52 | subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111438770 PE... | [more] |
| A0A6J1EW82 | 0.0 | 79.69 | subtilisin-like protease SBT5.3 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC1... | [more] |
| A0A6J1F1I2 | 0.0 | 79.30 | subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1... | [more] |
| A0A6J1EWV1 | 0.0 | 79.17 | subtilisin-like protease SBT5.3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... | [more] |
