Csor.00g030440 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g030440 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 4.6e-255
Identity = 461/769 (59.95%), Postives = 550/769 (71.52%), Query Frame = 0

Query: 1   MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLG 60
           M   +L  LLL   L +L  +   A KKSYIVYLGSH+H    S+  L  +  +H   L 
Sbjct: 16  MSLQSLSSLLL---LVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLA 75

Query: 61  SMLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN 120
           S +GS+E AKEAIFYSY RHIN FAA+LD+    +IAKHPDVVSV+ NK  KLHTT SWN
Sbjct: 76  SFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWN 135

Query: 121 FLRVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           F+ +  NG +   SLW  + +GE TII NLD+GVWPESKSFSDEGYG +P RWKG C   
Sbjct: 136 FMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH-- 195

Query: 181 SNFRCNRKLIGARYFNKGYASNVG-PLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFG 240
            +  CNRKLIGARYFNKGY +  G P N+SYET RD DGHG+HTLSTA GNFV   ++FG
Sbjct: 196 KDVPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFG 255

Query: 241 NGYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIE 300
            G GTA GGSPKARVAAY+VCWP V    CF ADILA  EAAI DGVDVLS S+GG A +
Sbjct: 256 IGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGD 315

Query: 301 FSEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLG 360
           +  D +AI S+HAVKNG+TVVCSAGNSGP +GTVSNVAPW+ITVGAS++DR F + V L 
Sbjct: 316 YMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELK 375

Query: 361 DKRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCL 420
           + + FKG SL SK LP +K Y LI A DA   N +  DA+LC++GSLDPKKVKGKI+VCL
Sbjct: 376 NGQSFKGTSL-SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCL 435

Query: 421 RGDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQST 480
           RGDNAR++KG   A AGA GM+L NDK +G+   +DAH+LP S I Y DGE+++ Y+ ST
Sbjct: 436 RGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSST 495

Query: 481 KTPIAYVTQVT-ELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSIT 540
           K P  Y+   T  L  KPAP MASFSSRGPNTI P ILKPDITAPGVNIIAAF+EAT  T
Sbjct: 496 KDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPT 555

Query: 541 GLPYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNP 600
            L  D RR  F   SGTSMSCPHISG+VGLLKTLHP WSPAAIRSAIMTT+ T  N   P
Sbjct: 556 DLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKP 615

Query: 601 ILTSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTS 660
           ++    +KANP +YG+GHVQPNKAA+PGLVYDL+T DYL+FLCA GYN   ++LF  D  
Sbjct: 616 MVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQ 675

Query: 661 FVCSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPST 720
           + C +   ++D NYPSI++ NL    + + R++KNVG P TY A+   P GV VSV P  
Sbjct: 676 YTCRQGANLLDFNYPSITVPNLTGS-ITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQ 735

Query: 721 LKFTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTL 768
           L F KTGE K F++ LR +    +  +VFG+L W+   H V SPI V L
Sbjct: 736 LTFNKTGEVKIFQMTLRPLPVTPS-GYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Csor.00g030440 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 2.0e-250
Identity = 439/763 (57.54%), Postives = 550/763 (72.08%), Query Frame = 0

Query: 10  LLSFFLFSLLQTSTIAAK--KSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLGSMLGSYE 69
           LL   L  +     +A+K   SY+VY G+HSH    +   +  + +THY+ LGS  GS E
Sbjct: 10  LLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRE 69

Query: 70  AAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWNFLRVENN 129
            A +AIFYSY +HIN FAA LD  +  +I+KHP+VVSV+ NK LKLHTTRSW+FL +E+N
Sbjct: 70  RATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHN 129

Query: 130 GGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEG--GSNFRC 189
             +PS S+W+ +RFGE TII NLD+GVWPESKSF DEG GPIP+RWKG C+    + F C
Sbjct: 130 SYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHC 189

Query: 190 NRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGNGYGTA 249
           NRKLIGARYFNKGYA+ VG LNSS+++ RD DGHG+HTLSTA G+FV  VS+FG G GTA
Sbjct: 190 NRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTA 249

Query: 250 KGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLL 309
           KGGSP+ARVAAY+VCWP V    C+ AD+LA F+AAI DG DV+S+SLGG    F  D +
Sbjct: 250 KGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSV 309

Query: 310 AIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGDKRHFK 369
           AI S+HA K  I VVCSAGNSGP   TVSNVAPW ITVGAST+DR F S++VLG+ +H+K
Sbjct: 310 AIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYK 369

Query: 370 GKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLRGDNAR 429
           G+SLSS  LP  KFYP++ +++AK  N S  DA LC+ GSLDP K KGKI+VCLRG N R
Sbjct: 370 GQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGR 429

Query: 430 IEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTKTPIAY 489
           +EKG  VA  G +GM+L N    G+   AD H+LP + +   D  +V +YI  TK PIA+
Sbjct: 430 VEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAH 489

Query: 490 VT-QVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGLPYDK 549
           +T   T+LG+KPAP+MASFSS+GP+ + P ILKPDITAPGV++IAA++ A S T   +D 
Sbjct: 490 ITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDP 549

Query: 550 RRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPILTSEK 609
           RR+ F  +SGTSMSCPHISGI GLLKT +P WSPAAIRSAIMTTA    +   PI  +  
Sbjct: 550 RRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATN 609

Query: 610 EKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFVCSKS 669
            KA P ++GAGHVQPN A NPGLVYDL  +DYLNFLC+ GYN +Q+ +F+ +     S  
Sbjct: 610 MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPK 669

Query: 670 FKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPSTLKFTKT 729
             +++LNYPSI++ NL S  V + R VKNVG P  Y  KV +P GV V+V P++L FTK 
Sbjct: 670 ISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKV 729

Query: 730 GEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTL 768
           GE+K FKV+L + + N  + +VFG+LVWS  KHRV SPI V L
Sbjct: 730 GEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Csor.00g030440 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 731.9 bits (1888), Expect = 7.6e-210
Identity = 403/786 (51.27%), Postives = 521/786 (66.28%), Query Frame = 0

Query: 4   FNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLGSML 63
           F L  +L SFFLF+ L  +   +KK YIVY+G+HSHG +P++ DL+L T +HY+LLGS+ 
Sbjct: 6   FCLHLILSSFFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIF 65

Query: 64  GSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWNFLR 123
           GS E AKEAI YSYNRHIN FAA+L+++   DIAK+P+VVSV+ +KE KLHTTRSW FL 
Sbjct: 66  GSREKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLG 125

Query: 124 VENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGS-CE---- 183
           +   G    +S W+  RFGE+TIIGN+D+GVWPES+SFSD+GYG +P++W+G  C+    
Sbjct: 126 LHRRG---QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKL 185

Query: 184 -GGSNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSM 243
            G     CNRKLIGARY+NK + ++ G L+    TARD  GHGTHTLSTAGGNFV    +
Sbjct: 186 PGSMKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARV 245

Query: 244 FGNGYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGA 303
           F  G GTAKGGSP+ARVAAY+VCW       C+ AD+LA  + AI DGVDV+++S G   
Sbjct: 246 FAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSY 305

Query: 304 IEFSE----DLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFT 363
           +  +E    D ++I ++HA+   I +V SAGN GP  GTV+NVAPW+ T+ AST+DR F+
Sbjct: 306 VVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFS 365

Query: 364 SHVVLGDKRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKG 423
           S++ + ++   +G SL    LP  + + LI + DAK  N +  DA LCR+G+LD  KV G
Sbjct: 366 SNLTINNQL-IEGASLFVN-LPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNG 425

Query: 424 KIVVCLR-GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESV 483
           KIV+C R G    + +G     AGA GMIL N  +NG    A+ H+  T +      +S 
Sbjct: 426 KIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSR 485

Query: 484 YQYIQST---------KT--PIAYVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDIT 543
              +++T         KT   I      T  G KPAP+MASFSSRGPN I+PSILKPD+T
Sbjct: 486 PHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVT 545

Query: 544 APGVNIIAAFSEATSITGLPYDKRR-VQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAA 603
           APGVNI+AA+SE  S + L  D RR  +F  L GTSMSCPH SGI GLLKT HP WSPAA
Sbjct: 546 APGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAA 605

Query: 604 IRSAIMTTAETEANDLNPILTS-EKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNF 663
           I+SAIMTTA T  N   PI  + +K  A+  AYG+GHV+P+ A  PGLVYDLS  DYLNF
Sbjct: 606 IKSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNF 665

Query: 664 LCARGYNKAQMKLFTNDTSFVCSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGT 723
           LCA GY++  +     + +F+CS S  V DLNYPSI++ NL+ + V I R V NVG P T
Sbjct: 666 LCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPST 725

Query: 724 YVAKVESPPGVSVSVVPSTLKFTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRV 766
           Y     SP G S++VVP +L FTK GE K FKV+++   +    ++ FG L W+ GKH V
Sbjct: 726 YTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIV 785

BLAST of Csor.00g030440 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 1.5e-176
Identity = 352/769 (45.77%), Postives = 486/769 (63.20%), Query Frame = 0

Query: 12  SFFLFSLLQTSTIAAKKS----YIVYLGSHSHGLNPSAVDLQLITQTHYNLLGSMLGSYE 71
           +FFL   L    +++  S    YIV++        PS+ DL      H N   S L S  
Sbjct: 10  AFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQ---MPSSFDL------HSNWYDSSLRSIS 69

Query: 72  AAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWNFLRVENN 131
            + E + Y+Y   I+ F+  L Q+  + +   P V+SV      +LHTTR+  FL ++ +
Sbjct: 70  DSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH 129

Query: 132 GGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGGSNFR--- 191
               +  L+  +      ++G LD+GVWPESKS+SDEG+GPIP+ WKG CE G+NF    
Sbjct: 130 ----TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASL 189

Query: 192 CNRKLIGARYFNKGYASNVGPLNSSYE--TARDDDGHGTHTLSTAGGNFVQRVSMFGNGY 251
           CNRKLIGAR+F +GY S +GP++ S E  + RDDDGHGTHT STA G+ V+  S+ G   
Sbjct: 190 CNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYAS 249

Query: 252 GTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSE 311
           GTA+G +P+ARVA Y+VCW     GGCF +DILA  + AI+D V+VLS+SLGGG  ++  
Sbjct: 250 GTARGMAPRARVAVYKVCW----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYR 309

Query: 312 DLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGDKR 371
           D +AI ++ A++ GI V CSAGN+GP + ++SNVAPW+ TVGA T+DR F +  +LG+ +
Sbjct: 310 DGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGK 369

Query: 372 HFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLRGD 431
           +F G SL        K  P I A +A  +N ++ +  LC  G+L P+KVKGKIV+C RG 
Sbjct: 370 NFTGVSLFKGEALPDKLLPFIYAGNA--SNATNGN--LCMTGTLIPEKVKGKIVMCDRGI 429

Query: 432 NARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTKTP 491
           NAR++KG VV  AG VGMILAN   NG+   ADAHLLP + +G   G+ +  Y+ +   P
Sbjct: 430 NARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNP 489

Query: 492 IAYVTQV-TELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGLP 551
            A ++ + T +G+KP+P++A+FSSRGPN+I P+ILKPD+ APGVNI+AA++ A   TGL 
Sbjct: 490 TASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLA 549

Query: 552 YDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPILT 611
            D RRV+F  +SGTSMSCPH+SG+  LLK++HP+WSPAAIRSA+MTTA     D  P+L 
Sbjct: 550 SDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLD 609

Query: 612 SEKEK-ANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV 671
               K + P  +GAGHV P  A NPGL+YDL+T+DYL FLCA  Y   Q++  +   ++ 
Sbjct: 610 IATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYT 669

Query: 672 C--SKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVES-PPGVSVSVVPS 731
           C  SKS+ V DLNYPS ++           R V +VG  GTY  KV S   GV +SV P+
Sbjct: 670 CDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPA 729

Query: 732 TLKFTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVT 767
            L F +  E+K++ V      S  +  + FG + WS GKH V SP+ ++
Sbjct: 730 VLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Csor.00g030440 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 2.2e-161
Identity = 343/782 (43.86%), Postives = 470/782 (60.10%), Query Frame = 0

Query: 9   LLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLGSMLGSYEA 68
           +LL F  F  +  +   A K++I  +     G  PS      I  THY+       S E 
Sbjct: 7   VLLLFLSFPFISFAASQAAKTFIFRIDG---GSMPS------IFPTHYHWY-----STEF 66

Query: 69  AKEA-IFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWNFLRVENN 128
           A+E+ I + Y+   + F+AV+     +++  HP V++V+E++  +LHTTRS  FL ++N 
Sbjct: 67  AEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQ 126

Query: 129 GGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGGSNF---R 188
            G     LW  S +G   IIG  D+G+WPE +SFSD   GPIP RW+G CE G+ F    
Sbjct: 127 KG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRN 186

Query: 189 CNRKLIGARYFNKG-YASNVGPLNSSYE--TARDDDGHGTHTLSTAGGNFVQRVSMFGNG 248
           CNRK+IGAR+F KG  A+ +G +N + E  + RD DGHGTHT STA G    + SM G  
Sbjct: 187 CNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYA 246

Query: 249 YGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGG---AI 308
            G AKG +PKAR+AAY+VCW D    GC  +DILA F+AA+ DGVDV+SIS+GGG     
Sbjct: 247 SGVAKGVAPKARIAAYKVCWKD---SGCLDSDILAAFDAAVRDGVDVISISIGGGDGITS 306

Query: 309 EFSEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVL 368
            +  D +AI SY A   GI V  SAGN GP   +V+N+APW+ TVGAST+DR F +  +L
Sbjct: 307 PYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAIL 366

Query: 369 GDKRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVC 428
           GD    +G SL + +    + +P++    +  ++ S     LC + +LDPK+V+GKIV+C
Sbjct: 367 GDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLDPKQVRGKIVIC 426

Query: 429 LRGDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQS 488
            RG + R+ KG VV +AG VGMILAN   NG+    DAHL+P   +G  +G+ +  Y  S
Sbjct: 427 DRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASS 486

Query: 489 TKTPIAYVT-QVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSI 548
              PIA +  + T +GIKPAP++ASFS RGPN + P ILKPD+ APGVNI+AA+++A   
Sbjct: 487 HPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGP 546

Query: 549 TGLPYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLN 608
           TGLP D R+ +F  LSGTSM+CPH+SG   LLK+ HP WSPA IRSA+MTT     N   
Sbjct: 547 TGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNR 606

Query: 609 PIL-TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTND 668
            ++  S  + A P  YG+GH+   +A NPGLVYD++  DY+ FLC+ GY    +++ T  
Sbjct: 607 SLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITR- 666

Query: 669 TSFVCSKSFKVI--DLNYPSISIY---NLKSEV-VNIKRRVKNVG-SPGTYVAKVESPPG 728
           T   C  + K    +LNYPSI+     N +  V   + R   NVG +   Y A++ESP G
Sbjct: 667 TPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRG 726

Query: 729 VSVSVVPSTLKFTKTGEEKNFKVVL----RRVQSNQTEEHVFGKLVW-SHGKHRVSSPIF 767
           V+V+V P  L FT   + +++ V +    R V   +T   VFG + W   GKH V SPI 
Sbjct: 727 VTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGA-VFGSVTWFDGGKHVVRSPIV 759

BLAST of Csor.00g030440 vs. NCBI nr
Match: KAG6577554.1 (Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1536 bits (3978), Expect = 0.0
Identity = 769/769 (100.00%), Postives = 769/769 (100.00%), Query Frame = 0

Query: 1   MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLG 60
           MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLG
Sbjct: 1   MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLG 60

Query: 61  SMLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN 120
           SMLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN
Sbjct: 61  SMLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN 120

Query: 121 FLRVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FLRVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLRVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN 240
           SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN
Sbjct: 181 SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN 240

Query: 241 GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 300
           GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF
Sbjct: 241 GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 300

Query: 301 SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD 360
           SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD
Sbjct: 301 SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD 360

Query: 361 KRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR 420
           KRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR
Sbjct: 361 KRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR 420

Query: 421 GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTK 480
           GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTK
Sbjct: 421 GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTK 480

Query: 481 TPIAYVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL 540
           TPIAYVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL
Sbjct: 481 TPIAYVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL 540

Query: 541 PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPIL 600
           PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPIL
Sbjct: 541 PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPIL 600

Query: 601 TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV 660
           TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV
Sbjct: 601 TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV 660

Query: 661 CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPSTLK 720
           CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPSTLK
Sbjct: 661 CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPSTLK 720

Query: 721 FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI 769
           FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI
Sbjct: 721 FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI 769

BLAST of Csor.00g030440 vs. NCBI nr
Match: XP_022932387.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])

HSP 1 Score: 1506 bits (3900), Expect = 0.0
Identity = 755/769 (98.18%), Postives = 759/769 (98.70%), Query Frame = 0

Query: 1   MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLG 60
           MEAFNLPPLLLSFFLFSLLQTSTIAA KSYIVYLGSHSHGLNPS VDLQL TQTHYNLLG
Sbjct: 1   MEAFNLPPLLLSFFLFSLLQTSTIAANKSYIVYLGSHSHGLNPSTVDLQLTTQTHYNLLG 60

Query: 61  SMLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN 120
           S+LGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAK+PDVVSVYENKELKLHTTRSWN
Sbjct: 61  SVLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKYPDVVSVYENKELKLHTTRSWN 120

Query: 121 FLRVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FL VENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN 240
           SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQ VSMFGN
Sbjct: 181 SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQGVSMFGN 240

Query: 241 GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 300
           GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF
Sbjct: 241 GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 300

Query: 301 SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD 360
           SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD
Sbjct: 301 SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD 360

Query: 361 KRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR 420
           KRHFKGKSLSSKLL AKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR
Sbjct: 361 KRHFKGKSLSSKLLLAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR 420

Query: 421 GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTK 480
           GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHI YTDGESVYQYI+STK
Sbjct: 421 GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHISYTDGESVYQYIRSTK 480

Query: 481 TPIAYVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL 540
           TPIAYVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL
Sbjct: 481 TPIAYVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL 540

Query: 541 PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPIL 600
           PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTA TEANDLNPIL
Sbjct: 541 PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAGTEANDLNPIL 600

Query: 601 TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV 660
           TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV
Sbjct: 601 TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV 660

Query: 661 CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPSTLK 720
           CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESP GVS+SV PSTLK
Sbjct: 661 CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPSGVSISVAPSTLK 720

Query: 721 FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI 769
           FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI
Sbjct: 721 FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI 769

BLAST of Csor.00g030440 vs. NCBI nr
Match: XP_023553405.1 (subtilisin-like protease SBT5.4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1492 bits (3863), Expect = 0.0
Identity = 747/769 (97.14%), Postives = 757/769 (98.44%), Query Frame = 0

Query: 1   MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLG 60
           MEAFNLPPLLLSFFLF+LLQTSTIAA KSYIVYLGSHSHGLNPSAVDLQL TQTHYNLLG
Sbjct: 1   MEAFNLPPLLLSFFLFALLQTSTIAANKSYIVYLGSHSHGLNPSAVDLQLTTQTHYNLLG 60

Query: 61  SMLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN 120
           S+LGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN
Sbjct: 61  SVLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN 120

Query: 121 FLRVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FL VENNGGIPS+SLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENNGGIPSNSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN 240
           SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN
Sbjct: 181 SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN 240

Query: 241 GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 300
           GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF
Sbjct: 241 GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 300

Query: 301 SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD 360
           SEDLLAIMSYHAVKN ITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD
Sbjct: 301 SEDLLAIMSYHAVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD 360

Query: 361 KRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR 420
           KRHFKGKSLSSKLLPAKK YPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR
Sbjct: 361 KRHFKGKSLSSKLLPAKKLYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR 420

Query: 421 GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTK 480
           G+NA+IEKGYVVAQAGAVGMILANDKENGDAFEA+AHLLPTSHI YTDGESVYQYIQSTK
Sbjct: 421 GENAKIEKGYVVAQAGAVGMILANDKENGDAFEANAHLLPTSHISYTDGESVYQYIQSTK 480

Query: 481 TPIAYVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL 540
           TP+AY+T VTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL
Sbjct: 481 TPMAYMTHVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL 540

Query: 541 PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPIL 600
           PYDKRRVQFTELSGTSMSCPHISGIVGLLKTL+PKWSPAAIRSAIMTTA TEANDLNPIL
Sbjct: 541 PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPIL 600

Query: 601 TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV 660
           TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV
Sbjct: 601 TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV 660

Query: 661 CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPSTLK 720
           CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPP VSVSV PSTLK
Sbjct: 661 CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPRVSVSVAPSTLK 720

Query: 721 FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI 769
           FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVW  GKHRVSSPIFVTL+I
Sbjct: 721 FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWFDGKHRVSSPIFVTLEI 769

BLAST of Csor.00g030440 vs. NCBI nr
Match: XP_023553309.1 (subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1384 bits (3583), Expect = 0.0
Identity = 689/769 (89.60%), Postives = 724/769 (94.15%), Query Frame = 0

Query: 1   MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLG 60
           ME FNLPPLL+SFFL  LLQTSTIAAKKSYIVYLGSHSHGLNPSA+DLQL TQTHYNLLG
Sbjct: 1   MEFFNLPPLLVSFFLLVLLQTSTIAAKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60

Query: 61  SMLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN 120
           S+LGS EAAKEAIFYSYNRHINAFAAVLDQKVVEDIAK PDVVSVYENK L+LHTTRSWN
Sbjct: 61  SVLGSNEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKRPDVVSVYENKGLELHTTRSWN 120

Query: 121 FLRVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FL VEN+GGIPS+SLW LS+FGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPSNSLWSLSKFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN 240
           SNF CNRKLIGARYFNKGYASNVG LNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN
Sbjct: 181 SNFSCNRKLIGARYFNKGYASNVGHLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN 240

Query: 241 GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 300
           GYGTAKGGSPKA VAAY+VCWP VG+GGCFMADILA FEAAISDGVDVLSISLGGGA EF
Sbjct: 241 GYGTAKGGSPKALVAAYKVCWPSVGSGGCFMADILAAFEAAISDGVDVLSISLGGGAREF 300

Query: 301 SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD 360
           SED+LAIMS+HAVKNGITVVCSAGN GP  GT SNVAPWMITVGASTVDR FT++VVLGD
Sbjct: 301 SEDILAIMSFHAVKNGITVVCSAGNFGPLEGTASNVAPWMITVGASTVDRHFTTNVVLGD 360

Query: 361 KRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR 420
           KRHFKGKSLSSKLLPAKK YPLIRALDAK NNVSDNDAI C QGSL+P+K KGKIVVCLR
Sbjct: 361 KRHFKGKSLSSKLLPAKKLYPLIRALDAKSNNVSDNDAIQCHQGSLNPEKAKGKIVVCLR 420

Query: 421 GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTK 480
           GD+ R+EKGY VAQAG +GMILANDK +GDA   DAHLLP +HI YTDGES+YQYIQSTK
Sbjct: 421 GDSPRVEKGYAVAQAGGIGMILANDKGSGDALSLDAHLLPAAHISYTDGESIYQYIQSTK 480

Query: 481 TPIAYVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL 540
           +P+AY+T VTELGIKPAPIMA FSSRGPNT+EP ILKPDITAPGVNIIAAFSEATSITGL
Sbjct: 481 SPMAYMTHVTELGIKPAPIMAPFSSRGPNTVEPLILKPDITAPGVNIIAAFSEATSITGL 540

Query: 541 PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPIL 600
           PYDKRRVQFTELSGTSMSCPHISGIVGLLKTL+PKWSPAAIRSAIMTTA TEANDLNPIL
Sbjct: 541 PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAVTEANDLNPIL 600

Query: 601 TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV 660
           + EKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNK QMKLF+NDTSFV
Sbjct: 601 SFEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKKQMKLFSNDTSFV 660

Query: 661 CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPSTLK 720
           CSKSFKVIDLNYPSIS+YNLKSEVV IKRRVKNVGSPGTYVAKVE+PPGV VSV PSTLK
Sbjct: 661 CSKSFKVIDLNYPSISMYNLKSEVVKIKRRVKNVGSPGTYVAKVEAPPGVLVSVDPSTLK 720

Query: 721 FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI 769
           FTKT EEK+F++VLRRVQSNQTE+HVFGKLVWS GKHRVSSPIFVTL+I
Sbjct: 721 FTKTAEEKDFQIVLRRVQSNQTEKHVFGKLVWSDGKHRVSSPIFVTLEI 769

BLAST of Csor.00g030440 vs. NCBI nr
Match: KAG6577555.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1384 bits (3582), Expect = 0.0
Identity = 688/769 (89.47%), Postives = 723/769 (94.02%), Query Frame = 0

Query: 1   MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLG 60
           MEAFNLPPLL+SFFL  LLQTSTIAAKKSYIVYLGSHSHGLNPS +DLQL TQTHYNLLG
Sbjct: 1   MEAFNLPPLLVSFFLLVLLQTSTIAAKKSYIVYLGSHSHGLNPSTIDLQLATQTHYNLLG 60

Query: 61  SMLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN 120
           S+LGS EAAKEAIFYSYNRHINAFAA+LDQKV EDIAK PDV SVYENK L+LHTTRSWN
Sbjct: 61  SVLGSNEAAKEAIFYSYNRHINAFAAILDQKVAEDIAKRPDVASVYENKGLELHTTRSWN 120

Query: 121 FLRVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FL VEN+GGIPS+SLW LS+FGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPSNSLWNLSKFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN 240
           SNF CNRKLIGARYFNKGYASN G LNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN
Sbjct: 181 SNFSCNRKLIGARYFNKGYASNGGHLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN 240

Query: 241 GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 300
           GYGTAKGGSPKA VAAY+VCWP V +GGCFMADILAGFEAAISDGVDVLSISLGGGA EF
Sbjct: 241 GYGTAKGGSPKALVAAYKVCWPAVASGGCFMADILAGFEAAISDGVDVLSISLGGGAREF 300

Query: 301 SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD 360
           SED+LAIMS+HAVKNGITVVCSAGN GP  GTVSNVAPWMITVGASTVDR FT++VVLGD
Sbjct: 301 SEDILAIMSFHAVKNGITVVCSAGNFGPLEGTVSNVAPWMITVGASTVDRHFTTNVVLGD 360

Query: 361 KRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR 420
           KRHFKGKSLSSKLLPAKK YPLIRALDAK NNVSDNDAILC QGSL+P+K KGKIVVCLR
Sbjct: 361 KRHFKGKSLSSKLLPAKKLYPLIRALDAKSNNVSDNDAILCHQGSLNPEKAKGKIVVCLR 420

Query: 421 GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTK 480
           GDN R+EKGYVVAQAG +GMILANDK +GDA   DAHLLP +HI YTDGES+YQYIQSTK
Sbjct: 421 GDNTRVEKGYVVAQAGGIGMILANDKGSGDALSLDAHLLPAAHISYTDGESIYQYIQSTK 480

Query: 481 TPIAYVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL 540
           +P+AY+T VTELGIKPAPIMA FSSRGPNT+EP ILKPDITAPGVNIIAAFSEATSITGL
Sbjct: 481 SPMAYMTHVTELGIKPAPIMAPFSSRGPNTVEPLILKPDITAPGVNIIAAFSEATSITGL 540

Query: 541 PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPIL 600
           PYDKRRVQFTELSGTSMSCPHISGIVGLLKTL+PKWSPAAIRSAIMTTA TEANDLNPIL
Sbjct: 541 PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAVTEANDLNPIL 600

Query: 601 TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV 660
           + E EKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNK QMKLF+NDTSFV
Sbjct: 601 SLENEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKKQMKLFSNDTSFV 660

Query: 661 CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPSTLK 720
           CSKSFKVIDLNYPSIS+YNLKSEVV IKRRVKNVGSPGTYVAKVE+PPGV VSV PSTLK
Sbjct: 661 CSKSFKVIDLNYPSISMYNLKSEVVKIKRRVKNVGSPGTYVAKVEAPPGVLVSVDPSTLK 720

Query: 721 FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI 769
           FTKT EEK+F+VVLRR+QSNQTE+HVFGKLVWS GKHRVSSPIFVTL+I
Sbjct: 721 FTKTAEEKDFQVVLRRIQSNQTEKHVFGKLVWSDGKHRVSSPIFVTLEI 769

BLAST of Csor.00g030440 vs. ExPASy TrEMBL
Match: A0A6J1EWJ2 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111438754 PE=3 SV=1)

HSP 1 Score: 1506 bits (3900), Expect = 0.0
Identity = 755/769 (98.18%), Postives = 759/769 (98.70%), Query Frame = 0

Query: 1   MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLG 60
           MEAFNLPPLLLSFFLFSLLQTSTIAA KSYIVYLGSHSHGLNPS VDLQL TQTHYNLLG
Sbjct: 1   MEAFNLPPLLLSFFLFSLLQTSTIAANKSYIVYLGSHSHGLNPSTVDLQLTTQTHYNLLG 60

Query: 61  SMLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN 120
           S+LGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAK+PDVVSVYENKELKLHTTRSWN
Sbjct: 61  SVLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKYPDVVSVYENKELKLHTTRSWN 120

Query: 121 FLRVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FL VENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN 240
           SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQ VSMFGN
Sbjct: 181 SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQGVSMFGN 240

Query: 241 GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 300
           GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF
Sbjct: 241 GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 300

Query: 301 SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD 360
           SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD
Sbjct: 301 SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD 360

Query: 361 KRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR 420
           KRHFKGKSLSSKLL AKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR
Sbjct: 361 KRHFKGKSLSSKLLLAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR 420

Query: 421 GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTK 480
           GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHI YTDGESVYQYI+STK
Sbjct: 421 GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHISYTDGESVYQYIRSTK 480

Query: 481 TPIAYVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL 540
           TPIAYVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL
Sbjct: 481 TPIAYVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL 540

Query: 541 PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPIL 600
           PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTA TEANDLNPIL
Sbjct: 541 PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAGTEANDLNPIL 600

Query: 601 TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV 660
           TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV
Sbjct: 601 TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV 660

Query: 661 CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPSTLK 720
           CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESP GVS+SV PSTLK
Sbjct: 661 CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPSGVSISVAPSTLK 720

Query: 721 FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI 769
           FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI
Sbjct: 721 FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI 769

BLAST of Csor.00g030440 vs. ExPASy TrEMBL
Match: A0A6J1L1F3 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111500242 PE=3 SV=1)

HSP 1 Score: 1318 bits (3412), Expect = 0.0
Identity = 663/769 (86.22%), Postives = 705/769 (91.68%), Query Frame = 0

Query: 1   MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLG 60
           MEAFNLPPLLLSF LF LLQTSTIAAKKSYIVYLGSHSHGLNPSA+DLQ  TQTHYNLLG
Sbjct: 1   MEAFNLPPLLLSF-LFVLLQTSTIAAKKSYIVYLGSHSHGLNPSAIDLQRATQTHYNLLG 60

Query: 61  SMLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN 120
           SMLGS EAAKEAIFYSYNRHINAFAAVLDQKV EDIAKH DVVSV+ENK+LKLHTTRSWN
Sbjct: 61  SMLGSNEAAKEAIFYSYNRHINAFAAVLDQKVAEDIAKHHDVVSVHENKKLKLHTTRSWN 120

Query: 121 FLRVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FL VEN+GGIP DSLW LSRFGESTIIGN+DSGVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPLDSLWNLSRFGESTIIGNIDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN 240
           ++F CNRKLIG RYFNKG+AS+VGPLNSSYETARD DGHGTHTLSTAGGNFV+ VS+ GN
Sbjct: 181 THFSCNRKLIGVRYFNKGFASDVGPLNSSYETARDVDGHGTHTLSTAGGNFVKGVSILGN 240

Query: 241 GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 300
            YGTAKGGSPKA VAAY+VCW      GCFMADILA FEAAISDGVDVLS+SLGGG  EF
Sbjct: 241 SYGTAKGGSPKALVAAYKVCWSTDRGDGCFMADILASFEAAISDGVDVLSVSLGGGIQEF 300

Query: 301 SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD 360
           S+DL+AI S+HAVKNGITVVCSAGNSGP   TV NVAPWMITVGASTVDRLFTS+VVLGD
Sbjct: 301 SDDLIAIGSFHAVKNGITVVCSAGNSGPSEDTVLNVAPWMITVGASTVDRLFTSYVVLGD 360

Query: 361 KRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR 420
           KR FKG+SLSSKLLP KKFYPLIRALDAK NN S+++AILCRQGSL+P+KV+GKIVVCLR
Sbjct: 361 KRQFKGESLSSKLLPPKKFYPLIRALDAKSNNTSNHEAILCRQGSLNPEKVRGKIVVCLR 420

Query: 421 GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTK 480
           G+N+R EKGYVVAQAG VGMILANDKE+GD   A  H LP SHI YTDGESVYQYIQST 
Sbjct: 421 GENSRGEKGYVVAQAGGVGMILANDKESGDELSASPHFLPASHISYTDGESVYQYIQSTN 480

Query: 481 TPIAYVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL 540
           TPIAY+T VTELGIKPAPIMASFSSRGPN +EPSILKPDITAPGVNIIAAFSEATSI+GL
Sbjct: 481 TPIAYMTSVTELGIKPAPIMASFSSRGPNIVEPSILKPDITAPGVNIIAAFSEATSISGL 540

Query: 541 PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPIL 600
           PYDKR+ QF  LSGTSMSCPHISGIVGLLKTL+PKWSPAAIRSAIMTTAETEANDLNPIL
Sbjct: 541 PYDKRQAQFITLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETEANDLNPIL 600

Query: 601 TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV 660
           TSEKEKANPLAYGAGHVQPNKA+NPGLVYDL+TQDYLNFLCARGYNK  +KLFTNDTSFV
Sbjct: 601 TSEKEKANPLAYGAGHVQPNKASNPGLVYDLTTQDYLNFLCARGYNKTLLKLFTNDTSFV 660

Query: 661 CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPSTLK 720
           CSKSFKV DLNYPSIS+ NLKSE V IKRRVKNVGSPG YVA+VE+PPGVSVSV PSTLK
Sbjct: 661 CSKSFKVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPGMYVAQVEAPPGVSVSVDPSTLK 720

Query: 721 FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI 769
           FTKT EEK+FKVVLRRV +NQTE++VFGKLVWS GKHRVSSPIFV L I
Sbjct: 721 FTKTDEEKDFKVVLRRVPNNQTEQNVFGKLVWSDGKHRVSSPIFVILGI 768

BLAST of Csor.00g030440 vs. ExPASy TrEMBL
Match: A0A6J1F219 (subtilisin-like protease SBT5.3 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111438753 PE=3 SV=1)

HSP 1 Score: 1317 bits (3409), Expect = 0.0
Identity = 660/769 (85.83%), Postives = 702/769 (91.29%), Query Frame = 0

Query: 1   MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLG 60
           MEAFNLPPLLLSFFLF LLQTSTIAAKKSYIVYLGSHSHGLNPSA+DLQL TQTHYNLLG
Sbjct: 1   MEAFNLPPLLLSFFLFVLLQTSTIAAKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60

Query: 61  SMLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN 120
           SMLGS EAAKEAIFYSYNRHINAFAAVLDQKV EDIAKH DVVSV+ENK+LKLHTTRSWN
Sbjct: 61  SMLGSNEAAKEAIFYSYNRHINAFAAVLDQKVAEDIAKHHDVVSVHENKKLKLHTTRSWN 120

Query: 121 FLRVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FL VEN+GGIP +SLW LSRFGESTIIGN+DSGVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPLNSLWNLSRFGESTIIGNIDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN 240
           +NF CNRKLIGARYFNKG+AS+ GPLNSSYETARD DGHGTHTLSTAGGNFV+ VS+ GN
Sbjct: 181 TNFSCNRKLIGARYFNKGFASDTGPLNSSYETARDVDGHGTHTLSTAGGNFVKGVSILGN 240

Query: 241 GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 300
            YGTAKGGSP+A VAAY+VCW      GCFMADILA FEAAISDGVDVLS+SLGGG  EF
Sbjct: 241 SYGTAKGGSPQALVAAYKVCWSTDTGDGCFMADILASFEAAISDGVDVLSVSLGGGIQEF 300

Query: 301 SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD 360
           S+DL+AI S+HAVKNGITVVCSA NSGP  GTV NVAPW+ITVGAST+DRLFTS+VVLGD
Sbjct: 301 SDDLIAIGSFHAVKNGITVVCSAANSGPSEGTVLNVAPWIITVGASTIDRLFTSYVVLGD 360

Query: 361 KRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR 420
           KR FKG+SLSS+LLP KKFYPLIRALDAK NN S  +AILCR+GSL+P KVKGKIVVCLR
Sbjct: 361 KRQFKGESLSSQLLPPKKFYPLIRALDAKSNNTSSTEAILCRKGSLNPDKVKGKIVVCLR 420

Query: 421 GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTK 480
           G+N+R EKGYVVAQAG VGMILANDKE GD   A  H LP +HI YTDGESVYQYIQSTK
Sbjct: 421 GENSRGEKGYVVAQAGGVGMILANDKEYGDDLSASPHFLPAAHISYTDGESVYQYIQSTK 480

Query: 481 TPIAYVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGL 540
           T IAY+TQVTELGIKPAPIMASFSSRGPNT+EPSILKPDITAPGV+IIAAFSEA SIT L
Sbjct: 481 TSIAYMTQVTELGIKPAPIMASFSSRGPNTVEPSILKPDITAPGVDIIAAFSEAASITDL 540

Query: 541 PYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPIL 600
           PYD+R  QF  LSGTSMSCPHISGIVGLLKTL+P WSPAAIRSAIMTTA TEANDLNPIL
Sbjct: 541 PYDERHTQFITLSGTSMSCPHISGIVGLLKTLYPNWSPAAIRSAIMTTAGTEANDLNPIL 600

Query: 601 TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV 660
           +SEKEKANPLAYGAGHVQPNKA+NPGLVYDLSTQDYLNFLCARGYNK QMKLFTNDTSFV
Sbjct: 601 SSEKEKANPLAYGAGHVQPNKASNPGLVYDLSTQDYLNFLCARGYNKTQMKLFTNDTSFV 660

Query: 661 CSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPSTLK 720
           CSKSFKV DLNYPSIS+ NLKSE V IKRRVKNVGSPGTYVA+VE+PPGVSVSV P+TLK
Sbjct: 661 CSKSFKVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPGTYVAQVEAPPGVSVSVDPNTLK 720

Query: 721 FTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTLKI 769
           FTKT EEK+FKVVL+RV +NQTEEHVFGKLVWS GKHRVSSPIFV L I
Sbjct: 721 FTKTDEEKDFKVVLKRVSNNQTEEHVFGKLVWSDGKHRVSSPIFVILGI 769

BLAST of Csor.00g030440 vs. ExPASy TrEMBL
Match: A0A6J1F1I2 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111438753 PE=3 SV=1)

HSP 1 Score: 1304 bits (3375), Expect = 0.0
Identity = 649/768 (84.51%), Postives = 703/768 (91.54%), Query Frame = 0

Query: 1   MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLG 60
           MEAFNLPPLLLSFFLF LLQTSTIAAKKSYIVYLGSHSHGLNPSA+DLQL TQTHYNLLG
Sbjct: 1   MEAFNLPPLLLSFFLFVLLQTSTIAAKKSYIVYLGSHSHGLNPSAIDLQLATQTHYNLLG 60

Query: 61  SMLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN 120
           SMLGS EAAKEAIFYSYNRHINAFAAVLDQKV EDIAK PDVVSVYENK LKLHTTRSWN
Sbjct: 61  SMLGSNEAAKEAIFYSYNRHINAFAAVLDQKVAEDIAKRPDVVSVYENKGLKLHTTRSWN 120

Query: 121 FLRVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FL VEN+GGIPS+SLW LSRFGESTIIGNLD+GVWPESKSFSD+ YGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPSNSLWNLSRFGESTIIGNLDTGVWPESKSFSDKEYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN 240
           SNF CNRKLIGARYFNKGYAS  GPLNSSYETARDD+GHGTHTLSTAGGNFV+ VS+FGN
Sbjct: 181 SNFNCNRKLIGARYFNKGYASVAGPLNSSYETARDDNGHGTHTLSTAGGNFVEGVSIFGN 240

Query: 241 GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 300
           GYGTAKGGSPK+ VAAY+VCWP V +GGCF+ADILAGFEAAISDGVDVLS+SLGG   EF
Sbjct: 241 GYGTAKGGSPKSLVAAYKVCWPPVEDGGCFVADILAGFEAAISDGVDVLSVSLGGRVQEF 300

Query: 301 SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD 360
            +D+LAI S+HAVKNGITVVCSAGNSGP+ GT SNVAPWM+TVGAST+DRLFT++V LGD
Sbjct: 301 YDDILAIGSFHAVKNGITVVCSAGNSGPYEGTASNVAPWMLTVGASTIDRLFTTNVTLGD 360

Query: 361 KRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR 420
           KRHFKG+SLS K+LPAKKFYPLIRALDAKFNNVSD +  LC +G+LDPKKV+GKIVVCLR
Sbjct: 361 KRHFKGESLSGKILPAKKFYPLIRALDAKFNNVSDYETGLCLEGTLDPKKVEGKIVVCLR 420

Query: 421 GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTK 480
           GDN+R++KGYVVAQAG VGMILAND   G A  ++AH+LP SHI YTDGESVY+YIQSTK
Sbjct: 421 GDNSRVDKGYVVAQAGGVGMILANDLLGGRALLSEAHILPASHISYTDGESVYKYIQSTK 480

Query: 481 TPIAYVTQV-TELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITG 540
           TP+AY+T V TELGIKPAPIMASFSSRGPN IEP+ILKPDITAPGVNI+AAFS     T 
Sbjct: 481 TPMAYMTSVRTELGIKPAPIMASFSSRGPNVIEPTILKPDITAPGVNILAAFSNIAPPTH 540

Query: 541 LPYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPI 600
             +DKRRV +  LSGTSMSCPHISGIVGLLKTL+PKWSPAAIRSAIMTTAET+ANDLNPI
Sbjct: 541 SHFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETKANDLNPI 600

Query: 601 LTSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSF 660
           LTS +EKA P AYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNK QM+LFTNDTSF
Sbjct: 601 LTSIEEKAGPFAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKTQMRLFTNDTSF 660

Query: 661 VCSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPSTL 720
           VCSKSFKV DLNYPSISI++L+S+ VNIKRRVKNVGSPGTYVAKVE+PPG+ VSV PSTL
Sbjct: 661 VCSKSFKVTDLNYPSISIFDLRSKEVNIKRRVKNVGSPGTYVAKVEAPPGILVSVDPSTL 720

Query: 721 KFTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTL 767
           KFTK+ EEK+FKVVL+RV +NQTE HVFGKLVWS GKHRVSSPI VTL
Sbjct: 721 KFTKSDEEKDFKVVLKRVPNNQTENHVFGKLVWSDGKHRVSSPISVTL 768

BLAST of Csor.00g030440 vs. ExPASy TrEMBL
Match: A0A6J1EW82 (subtilisin-like protease SBT5.3 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111438753 PE=3 SV=1)

HSP 1 Score: 1302 bits (3370), Expect = 0.0
Identity = 647/768 (84.24%), Postives = 703/768 (91.54%), Query Frame = 0

Query: 1   MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLG 60
           MEAFNLPPLLLSFFLF+LLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQL TQTHYNLLG
Sbjct: 1   MEAFNLPPLLLSFFLFALLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLATQTHYNLLG 60

Query: 61  SMLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN 120
           S+LGS EAAKEAIFYSYNRHIN FAAVLDQKV ED+AK PDVVSVYENK LKLHTTRSWN
Sbjct: 61  SVLGSNEAAKEAIFYSYNRHINGFAAVLDQKVAEDVAKRPDVVSVYENKGLKLHTTRSWN 120

Query: 121 FLRVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FL VEN+GGIPS+SLW LSRFGESTIIGNLD+GVWPESKSFSD+ YGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPSNSLWNLSRFGESTIIGNLDTGVWPESKSFSDKEYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGN 240
           SNF CNRKLIGARYFNKGYAS  GPLNSSYETARDD+GHGTHTLSTAGGNFV+ VS+FGN
Sbjct: 181 SNFNCNRKLIGARYFNKGYASVAGPLNSSYETARDDNGHGTHTLSTAGGNFVEGVSIFGN 240

Query: 241 GYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 300
           GYGTAKGGSPK+ VAAY+VCWP V +GGCF+ADILAGFEAAISDGVDVLS+SLGG   EF
Sbjct: 241 GYGTAKGGSPKSLVAAYKVCWPPVEDGGCFVADILAGFEAAISDGVDVLSVSLGGRVQEF 300

Query: 301 SEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGD 360
            +D+LAI S+HAVKNGITVVCSAGNSGP+ GT SNVAPWM+TVGAST+DRLFT++V LGD
Sbjct: 301 YDDILAIGSFHAVKNGITVVCSAGNSGPYEGTASNVAPWMLTVGASTIDRLFTTNVTLGD 360

Query: 361 KRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLR 420
           KRHFKG+SLS K+LPAKKFYPLIRALDAKFNNVSD +  LC +G+LDPKKV+GKIVVCLR
Sbjct: 361 KRHFKGESLSGKILPAKKFYPLIRALDAKFNNVSDYETGLCLEGTLDPKKVEGKIVVCLR 420

Query: 421 GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTK 480
           GDN+R++KGYVVAQAG VGMILAND   G A  ++AH+LP SHI YTDGESVY+YIQSTK
Sbjct: 421 GDNSRVDKGYVVAQAGGVGMILANDLLGGRALLSEAHILPASHISYTDGESVYKYIQSTK 480

Query: 481 TPIAYVTQV-TELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITG 540
           TP+AY+T V TELGIKPAPIMASFSSRGPN IEP+ILKPDITAPGVNI+AAFS     T 
Sbjct: 481 TPMAYMTSVRTELGIKPAPIMASFSSRGPNVIEPTILKPDITAPGVNILAAFSNIAPPTH 540

Query: 541 LPYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPI 600
             +DKRRV +  LSGTSMSCPHISGIVGLLKTL+PKWSPAAIRSAIMTTAET+ANDLNPI
Sbjct: 541 SHFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETKANDLNPI 600

Query: 601 LTSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSF 660
           LTS +EKA P AYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNK QM+LFTNDTSF
Sbjct: 601 LTSIEEKAGPFAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKTQMRLFTNDTSF 660

Query: 661 VCSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPSTL 720
           VCSKSFKV DLNYPSISI++L+S+ VNIKRRVKNVGSPGTYVAKVE+PPG+ VSV PSTL
Sbjct: 661 VCSKSFKVTDLNYPSISIFDLRSKEVNIKRRVKNVGSPGTYVAKVEAPPGILVSVDPSTL 720

Query: 721 KFTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTL 767
           KFTK+ EEK+FKVVL+RV +NQTE HVFGKLVWS GKHRVSSPI VTL
Sbjct: 721 KFTKSDEEKDFKVVLKRVPNNQTENHVFGKLVWSDGKHRVSSPISVTL 768

BLAST of Csor.00g030440 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 882.1 bits (2278), Expect = 3.2e-256
Identity = 461/769 (59.95%), Postives = 550/769 (71.52%), Query Frame = 0

Query: 1   MEAFNLPPLLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLG 60
           M   +L  LLL   L +L  +   A KKSYIVYLGSH+H    S+  L  +  +H   L 
Sbjct: 16  MSLQSLSSLLL---LVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLA 75

Query: 61  SMLGSYEAAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWN 120
           S +GS+E AKEAIFYSY RHIN FAA+LD+    +IAKHPDVVSV+ NK  KLHTT SWN
Sbjct: 76  SFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWN 135

Query: 121 FLRVENNGGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           F+ +  NG +   SLW  + +GE TII NLD+GVWPESKSFSDEGYG +P RWKG C   
Sbjct: 136 FMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH-- 195

Query: 181 SNFRCNRKLIGARYFNKGYASNVG-PLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFG 240
            +  CNRKLIGARYFNKGY +  G P N+SYET RD DGHG+HTLSTA GNFV   ++FG
Sbjct: 196 KDVPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFG 255

Query: 241 NGYGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIE 300
            G GTA GGSPKARVAAY+VCWP V    CF ADILA  EAAI DGVDVLS S+GG A +
Sbjct: 256 IGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGD 315

Query: 301 FSEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLG 360
           +  D +AI S+HAVKNG+TVVCSAGNSGP +GTVSNVAPW+ITVGAS++DR F + V L 
Sbjct: 316 YMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELK 375

Query: 361 DKRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCL 420
           + + FKG SL SK LP +K Y LI A DA   N +  DA+LC++GSLDPKKVKGKI+VCL
Sbjct: 376 NGQSFKGTSL-SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCL 435

Query: 421 RGDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQST 480
           RGDNAR++KG   A AGA GM+L NDK +G+   +DAH+LP S I Y DGE+++ Y+ ST
Sbjct: 436 RGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSST 495

Query: 481 KTPIAYVTQVT-ELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSIT 540
           K P  Y+   T  L  KPAP MASFSSRGPNTI P ILKPDITAPGVNIIAAF+EAT  T
Sbjct: 496 KDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPT 555

Query: 541 GLPYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNP 600
            L  D RR  F   SGTSMSCPHISG+VGLLKTLHP WSPAAIRSAIMTT+ T  N   P
Sbjct: 556 DLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKP 615

Query: 601 ILTSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTS 660
           ++    +KANP +YG+GHVQPNKAA+PGLVYDL+T DYL+FLCA GYN   ++LF  D  
Sbjct: 616 MVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQ 675

Query: 661 FVCSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPST 720
           + C +   ++D NYPSI++ NL    + + R++KNVG P TY A+   P GV VSV P  
Sbjct: 676 YTCRQGANLLDFNYPSITVPNLTGS-ITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQ 735

Query: 721 LKFTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTL 768
           L F KTGE K F++ LR +    +  +VFG+L W+   H V SPI V L
Sbjct: 736 LTFNKTGEVKIFQMTLRPLPVTPS-GYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Csor.00g030440 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 866.7 bits (2238), Expect = 1.4e-251
Identity = 439/763 (57.54%), Postives = 550/763 (72.08%), Query Frame = 0

Query: 10  LLSFFLFSLLQTSTIAAK--KSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLGSMLGSYE 69
           LL   L  +     +A+K   SY+VY G+HSH    +   +  + +THY+ LGS  GS E
Sbjct: 10  LLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRE 69

Query: 70  AAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWNFLRVENN 129
            A +AIFYSY +HIN FAA LD  +  +I+KHP+VVSV+ NK LKLHTTRSW+FL +E+N
Sbjct: 70  RATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHN 129

Query: 130 GGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEG--GSNFRC 189
             +PS S+W+ +RFGE TII NLD+GVWPESKSF DEG GPIP+RWKG C+    + F C
Sbjct: 130 SYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHC 189

Query: 190 NRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSMFGNGYGTA 249
           NRKLIGARYFNKGYA+ VG LNSS+++ RD DGHG+HTLSTA G+FV  VS+FG G GTA
Sbjct: 190 NRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTA 249

Query: 250 KGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLL 309
           KGGSP+ARVAAY+VCWP V    C+ AD+LA F+AAI DG DV+S+SLGG    F  D +
Sbjct: 250 KGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSV 309

Query: 310 AIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGDKRHFK 369
           AI S+HA K  I VVCSAGNSGP   TVSNVAPW ITVGAST+DR F S++VLG+ +H+K
Sbjct: 310 AIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYK 369

Query: 370 GKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLRGDNAR 429
           G+SLSS  LP  KFYP++ +++AK  N S  DA LC+ GSLDP K KGKI+VCLRG N R
Sbjct: 370 GQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGR 429

Query: 430 IEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTKTPIAY 489
           +EKG  VA  G +GM+L N    G+   AD H+LP + +   D  +V +YI  TK PIA+
Sbjct: 430 VEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAH 489

Query: 490 VT-QVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGLPYDK 549
           +T   T+LG+KPAP+MASFSS+GP+ + P ILKPDITAPGV++IAA++ A S T   +D 
Sbjct: 490 ITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDP 549

Query: 550 RRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPILTSEK 609
           RR+ F  +SGTSMSCPHISGI GLLKT +P WSPAAIRSAIMTTA    +   PI  +  
Sbjct: 550 RRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATN 609

Query: 610 EKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFVCSKS 669
            KA P ++GAGHVQPN A NPGLVYDL  +DYLNFLC+ GYN +Q+ +F+ +     S  
Sbjct: 610 MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPK 669

Query: 670 FKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVSVVPSTLKFTKT 729
             +++LNYPSI++ NL S  V + R VKNVG P  Y  KV +P GV V+V P++L FTK 
Sbjct: 670 ISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKV 729

Query: 730 GEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVTL 768
           GE+K FKV+L + + N  + +VFG+LVWS  KHRV SPI V L
Sbjct: 730 GEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Csor.00g030440 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 621.3 bits (1601), Expect = 1.0e-177
Identity = 352/769 (45.77%), Postives = 486/769 (63.20%), Query Frame = 0

Query: 12  SFFLFSLLQTSTIAAKKS----YIVYLGSHSHGLNPSAVDLQLITQTHYNLLGSMLGSYE 71
           +FFL   L    +++  S    YIV++        PS+ DL      H N   S L S  
Sbjct: 10  AFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQ---MPSSFDL------HSNWYDSSLRSIS 69

Query: 72  AAKEAIFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWNFLRVENN 131
            + E + Y+Y   I+ F+  L Q+  + +   P V+SV      +LHTTR+  FL ++ +
Sbjct: 70  DSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH 129

Query: 132 GGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGGSNFR--- 191
               +  L+  +      ++G LD+GVWPESKS+SDEG+GPIP+ WKG CE G+NF    
Sbjct: 130 ----TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASL 189

Query: 192 CNRKLIGARYFNKGYASNVGPLNSSYE--TARDDDGHGTHTLSTAGGNFVQRVSMFGNGY 251
           CNRKLIGAR+F +GY S +GP++ S E  + RDDDGHGTHT STA G+ V+  S+ G   
Sbjct: 190 CNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYAS 249

Query: 252 GTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSE 311
           GTA+G +P+ARVA Y+VCW     GGCF +DILA  + AI+D V+VLS+SLGGG  ++  
Sbjct: 250 GTARGMAPRARVAVYKVCW----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYR 309

Query: 312 DLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGDKR 371
           D +AI ++ A++ GI V CSAGN+GP + ++SNVAPW+ TVGA T+DR F +  +LG+ +
Sbjct: 310 DGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGK 369

Query: 372 HFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLRGD 431
           +F G SL        K  P I A +A  +N ++ +  LC  G+L P+KVKGKIV+C RG 
Sbjct: 370 NFTGVSLFKGEALPDKLLPFIYAGNA--SNATNGN--LCMTGTLIPEKVKGKIVMCDRGI 429

Query: 432 NARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTKTP 491
           NAR++KG VV  AG VGMILAN   NG+   ADAHLLP + +G   G+ +  Y+ +   P
Sbjct: 430 NARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNP 489

Query: 492 IAYVTQV-TELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGLP 551
            A ++ + T +G+KP+P++A+FSSRGPN+I P+ILKPD+ APGVNI+AA++ A   TGL 
Sbjct: 490 TASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLA 549

Query: 552 YDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPILT 611
            D RRV+F  +SGTSMSCPH+SG+  LLK++HP+WSPAAIRSA+MTTA     D  P+L 
Sbjct: 550 SDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLD 609

Query: 612 SEKEK-ANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV 671
               K + P  +GAGHV P  A NPGL+YDL+T+DYL FLCA  Y   Q++  +   ++ 
Sbjct: 610 IATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYT 669

Query: 672 C--SKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVES-PPGVSVSVVPS 731
           C  SKS+ V DLNYPS ++           R V +VG  GTY  KV S   GV +SV P+
Sbjct: 670 CDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPA 729

Query: 732 TLKFTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSHGKHRVSSPIFVT 767
            L F +  E+K++ V      S  +  + FG + WS GKH V SP+ ++
Sbjct: 730 VLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Csor.00g030440 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 570.9 bits (1470), Expect = 1.6e-162
Identity = 332/766 (43.34%), Postives = 463/766 (60.44%), Query Frame = 0

Query: 10  LLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLGSMLGSYEAA 69
           +++ FLF LL T+   AKK+YI+ +   +H   P +        TH++   S L S    
Sbjct: 13  IITTFLFLLLHTT---AKKTYIIRV---NHSDKPESF------LTHHDWYTSQLNS---- 72

Query: 70  KEAIFYSYNRHINAFAAVLDQKVVED-IAKHPDVVSVYENKELKLHTTRSWNFLRVENNG 129
           + ++ Y+Y    + F+A LD    +  ++    ++ ++E+    LHTTR+  FL + +  
Sbjct: 73  ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEF 132

Query: 130 GIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGGSNF---RC 189
           G+       L       IIG LD+GVWPES+SF D     IP++WKG CE GS+F    C
Sbjct: 133 GVHD-----LGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLC 192

Query: 190 NRKLIGARYFNKGY-ASNVGPLNSSYETA--RDDDGHGTHTLSTAGGNFVQRVSMFGNGY 249
           N+KLIGAR F+KG+  ++ G  +S  E+   RD DGHGTHT +TA G+ V+  S  G   
Sbjct: 193 NKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAA 252

Query: 250 GTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSE 309
           GTA+G + +ARVA Y+VCW    + GCF +DILA  + AI DGVDVLS+SLGGG+  +  
Sbjct: 253 GTARGMATRARVATYKVCW----STGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYR 312

Query: 310 DLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVLGDKR 369
           D +AI ++ A++ G+ V CSAGNSGP   +V+NVAPW++TVGA T+DR F +   LG+ +
Sbjct: 313 DTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGK 372

Query: 370 HFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVCLRGD 429
              G SL S +    K       L+  +N  + + + LC  GSLD   V+GKIVVC RG 
Sbjct: 373 RLTGVSLYSGVGMGTK------PLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGV 432

Query: 430 NARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQSTKTP 489
           NAR+EKG VV  AG +GMI+AN   +G+   AD+HLLP   +G   G+ + +Y++S   P
Sbjct: 433 NARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKP 492

Query: 490 IA-YVTQVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSITGLP 549
            A  V + T L +KP+P++A+FSSRGPNT+ P ILKPD+  PGVNI+A +S+A   TGL 
Sbjct: 493 TALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLD 552

Query: 550 YDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLNPIL- 609
            D RR QF  +SGTSMSCPHISG+ GLLK  HP+WSP+AI+SA+MTTA    N   P+  
Sbjct: 553 KDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHD 612

Query: 610 TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTNDTSFV 669
            ++   +NP A+G+GHV P KA +PGLVYD+ST++Y+ FLC+  Y    +       S  
Sbjct: 613 AADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVN 672

Query: 670 CSKSFK-VIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGT-YVAKVESPPGVSVSVVPST 729
           CSK F     LNYPS S+      VV   R V NVG+  + Y   V   P V +SV PS 
Sbjct: 673 CSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSK 732

Query: 730 LKFTKTGEEKNFKVV-LRRVQSNQTEEHVFGKLVWSHGKHRVSSPI 764
           L F   GE+K + V  + +   + T +  FG + WS+ +H V SP+
Sbjct: 733 LSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPV 747

BLAST of Csor.00g030440 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 570.9 bits (1470), Expect = 1.6e-162
Identity = 343/782 (43.86%), Postives = 470/782 (60.10%), Query Frame = 0

Query: 9   LLLSFFLFSLLQTSTIAAKKSYIVYLGSHSHGLNPSAVDLQLITQTHYNLLGSMLGSYEA 68
           +LL F  F  +  +   A K++I  +     G  PS      I  THY+       S E 
Sbjct: 7   VLLLFLSFPFISFAASQAAKTFIFRIDG---GSMPS------IFPTHYHWY-----STEF 66

Query: 69  AKEA-IFYSYNRHINAFAAVLDQKVVEDIAKHPDVVSVYENKELKLHTTRSWNFLRVENN 128
           A+E+ I + Y+   + F+AV+     +++  HP V++V+E++  +LHTTRS  FL ++N 
Sbjct: 67  AEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQ 126

Query: 129 GGIPSDSLWKLSRFGESTIIGNLDSGVWPESKSFSDEGYGPIPTRWKGSCEGGSNF---R 188
            G     LW  S +G   IIG  D+G+WPE +SFSD   GPIP RW+G CE G+ F    
Sbjct: 127 KG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRN 186

Query: 189 CNRKLIGARYFNKG-YASNVGPLNSSYE--TARDDDGHGTHTLSTAGGNFVQRVSMFGNG 248
           CNRK+IGAR+F KG  A+ +G +N + E  + RD DGHGTHT STA G    + SM G  
Sbjct: 187 CNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYA 246

Query: 249 YGTAKGGSPKARVAAYRVCWPDVGNGGCFMADILAGFEAAISDGVDVLSISLGGG---AI 308
            G AKG +PKAR+AAY+VCW D    GC  +DILA F+AA+ DGVDV+SIS+GGG     
Sbjct: 247 SGVAKGVAPKARIAAYKVCWKD---SGCLDSDILAAFDAAVRDGVDVISISIGGGDGITS 306

Query: 309 EFSEDLLAIMSYHAVKNGITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSHVVL 368
            +  D +AI SY A   GI V  SAGN GP   +V+N+APW+ TVGAST+DR F +  +L
Sbjct: 307 PYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAIL 366

Query: 369 GDKRHFKGKSLSSKLLPAKKFYPLIRALDAKFNNVSDNDAILCRQGSLDPKKVKGKIVVC 428
           GD    +G SL + +    + +P++    +  ++ S     LC + +LDPK+V+GKIV+C
Sbjct: 367 GDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLDPKQVRGKIVIC 426

Query: 429 LRGDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPTSHIGYTDGESVYQYIQS 488
            RG + R+ KG VV +AG VGMILAN   NG+    DAHL+P   +G  +G+ +  Y  S
Sbjct: 427 DRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASS 486

Query: 489 TKTPIAYVT-QVTELGIKPAPIMASFSSRGPNTIEPSILKPDITAPGVNIIAAFSEATSI 548
              PIA +  + T +GIKPAP++ASFS RGPN + P ILKPD+ APGVNI+AA+++A   
Sbjct: 487 HPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGP 546

Query: 549 TGLPYDKRRVQFTELSGTSMSCPHISGIVGLLKTLHPKWSPAAIRSAIMTTAETEANDLN 608
           TGLP D R+ +F  LSGTSM+CPH+SG   LLK+ HP WSPA IRSA+MTT     N   
Sbjct: 547 TGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNR 606

Query: 609 PIL-TSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCARGYNKAQMKLFTND 668
            ++  S  + A P  YG+GH+   +A NPGLVYD++  DY+ FLC+ GY    +++ T  
Sbjct: 607 SLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITR- 666

Query: 669 TSFVCSKSFKVI--DLNYPSISIY---NLKSEV-VNIKRRVKNVG-SPGTYVAKVESPPG 728
           T   C  + K    +LNYPSI+     N +  V   + R   NVG +   Y A++ESP G
Sbjct: 667 TPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRG 726

Query: 729 VSVSVVPSTLKFTKTGEEKNFKVVL----RRVQSNQTEEHVFGKLVW-SHGKHRVSSPIF 767
           V+V+V P  L FT   + +++ V +    R V   +T   VFG + W   GKH V SPI 
Sbjct: 727 VTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGA-VFGSVTWFDGGKHVVRSPIV 759