Csor.00g028080 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g028080
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	Aminoacyl-tRNA hydrolase
Location	Csor_Chr20: 4731011 .. 4734564 (+)
RNA-Seq Expression	Csor.00g028080
Synteny	Csor.00g028080

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAACATAAGCACAATCGTCATTCAACGCCTGGTAATGTTTTTTCCTTTTTAACGTCTTTGATTCTTCCTTTCCAGAAAAGAATAAGTTCTTTTGTCAAGCGTCTCGCAAGTTTCTTTCAAGAACTCCGTTTATCTGGAATACGATGAAGGACTCGGATTGGTTAATTCAAAGTTGAATAGTGGGGAGTTAAGTTCGCATTATATGTTTTCTAAGAAATCGGTGTTTTCGAAAGCTGTAATGAAATAACATGGTAGGACTGTTTTGCAGTGAGGGCTTCTTGGATTGGTTTATCCTTTTATTTTGATATCTCTGTTAATTGGCTTGTTCTGAACTTTGGAGGCTGATTGATCAATTTTGAAACGGTAATAAAATAACATGGTATAAGAAAACGTTCTGTAGTTAATAATTGGGTATATTCACGATGCATTTTATAGATTGATTAAGAGTCGTATGATAACCATTTGATTTTTGAAAAATTAAGCTTACAAACACTAGTTACATCTATTGGTTTCTTTGTTTTATTGTCTACCTTTTAGGATTGATTTCAAATTCCAATCAAGTTTTCAACTAAAACAAGTTTTTGTTTTTAAGATTTGAGGAAATTGTGAGAAAACAAGCACAATATTTAAAAGCAAAAAACCAAATCGTTATCAAATGGGATCAGAAAATTAAATTCCTAAATGACATTCAAAAGGTTCTTTGTGTGTTCTGCAAAGAAAATGCAGGAAAAATCTAAATTTTTCTGTATATCATAATCTTGAATTTGAGGAAATGGCTGCTATGTCATTCATTTTATCTTTGTTCTCTTCCTTCTGTAGTTACTGTTTCTGAGTTCTAACTTGAGGTATTCCTGTTCTATGTAGGATTTCAATTGCTTGAATTAGAATTTGGTATCTGGTCCAGCAATAGCCTTGCTGGACTTCAATAGTAGAGGAGTGCATTTTATAGACTAGGATTGGATGGATCTGACATGGCTGAGTGCCATTGTAGTTGGGGCAAGCTGCCTTGTTTTGGGATACTGTATTGGTTCGAAGTATCCTCCTCGCATCTTTATCAAAGCTAAATTAGCCAAAATCAACCAATCCAGCAGAAATGGGAAGAAGGATAAGCCTAAAGAGCCATTGGAGGTCGAAAATTTGGCTGACATACTCGATGACTTCAAGATGGTCTGTCTCTCTGTTTCTCTCTTGCTGTCTATTGGAAAATCATTCTCGACGACTTTAAGACGTTCTCTCTTTTACTCTCTCTAAAAAAATATAACAACATTTCACTGTGTCTCTTCAGGTTTTAGTTGTGAGAAATGATTTGAAAATGGGAAAAGGAAAAATTGCTGCTCAATGCAGGTATTTTATTACCATCTTGTGGCTCCTCTTTCATTACTGATGTCTTTAATGCTAAAAATGATAGAAGTTTTAAATTTTACTGTTGCTTGCATACTATGAATGCTGGAGTTGGTTTTTATTAGAAACTAGTACTAAGAATTGGGCATATAATGTTTGGAAGTTTAGGGTAACAACCCACTGCACACCTGGTATAGTAAATTTATTGCAAAGATTTATAAGTTTAACTAAAATTATAACTAAAAGTATCAATGACTAACATAAGAGGTTTTGGATGATAATAGGCTGAAAGTCAAAGTTGTCACAATGCAAAATTTTGGATGATAATAGTGCTTCTATTTTTTGAAATATTGAGGAAAATTATTGATGAATGGAATCTATAAAACAACAGCAAAGGTGCTAATGGGAAGAATCATCCTCGTAAATAAAATTTCATGATGAAAGAAAGATGTATTGATTATGCACCCTTTTCACCTTTTTCTCAATTTCCAGACCAACCTGCTTTACTGTCAATGCTATATCCAAAAACTATGGATCCACTTTTTCATACCCAACCCTTTTCTTTCTTACATCACTTTTCTTCGGGTGCCCCTTTTCTTTACTATGAGGGTTACCCTAGGGAATGGTGGAGTCCTGAGAAAATTATCCTCTGAATTCCAGTCATTTATGAGATTAGTTTACTGTATAAGGAGGGATCTTTCGTCCATCACTGTCTAGGTGCTCTACTTTTGGTGGATCTCTCAACGGCGCTTTCTGGACATCTAATTTGAAGATTTATTGTCTGAATTTTTTGCTTGGTTGGTTGATAGTTGAATAATAGACAATAGGCATAATGAAACCATCAGTTAAATTTATTTAGTTGGAATATTGTAGCATACTTGTAGATTCTGGGTTTTATGGCCGTTTTTGTTTTTTTGTCCTTGGTAATGTGTGGTATCCTATCCTTCCACCACATTAAGGTATACAGATACCTTTCAATCCTCATGGCAGATTGCGATGGACATAATAGTTAAGCTAAAACTTGGTTGAGCTTGCATAGATGCAGTTTAACTGAAGGGATTTTGATGATAAGGAAGAAGAACCGTCAGAACTTTTATGGTACAAGGCATATAAGTGGAATATGCATTGAACACCCCTATGCATAGCTCTGATACCATGTTAGGAACCACGACCCTTTACGAAAAGAATTGCCAATACTAGACAAATTATGATACTATACTATTACTTGAGAGGCCAAATTTTCGTATGATTTAATATGAGTATTGACTGTTTAAGTACTGCCTTTTTACTTGTTTAAAAATTAAATGATTGAGAATTGAGCCTAGGAATCTAATTTCGTCAAACCTTTGCACAGTGAACTTCCCAGTTTCTTAATGCTGTAAATTATATTTGGACTAGTTTCTGATCCACTTAACTTGCTTTTGTTAGTCATGCAACTTTGGGTCTCTATAAGAAGCTCCATTATCGTGCACCAAAAGCGTTGAATAGGTACGTATTTACAGAATCGTCCTCCCTATATTAATGTGCATCCCATACTTCGTTCCCTGTACATTTATTTGATTTTATTTACATGTAGGTGGGAAATGTGTGGACAACCCAAGGTGGTACTGAAGATAGAAAGTGAGGAAGACATGCTAGTCTTGCAAGTAACAATTCCACTATGATTTCCTTTCTTAAAAGTTCATTTTATGGTATCTAGCTGATTTTTGTTATATGAAGTTCTCCCTAATATAATCCGATCGCGCTTTTCTATATCTCATCTATTCTTTGAGGCATTTCAGTAATTCTTTCATTTTTTCAAACTTTTGTATATTGTTGTTCATCAGGAGAGGGCAAAATCATTGAAGCTACCAACCCATATTACCATTGATGCCGGAAAAACTCAGATTGCTCCTAGTGAGTTAAATGAAATTCACCGAAGTAGATCGACTTTTTTTCACAGTAAATTTATCAACTTCACTAATTTTCACTTTTTTGTCTTTCCAGATTCAAGAACGGTGATGGCAATTCTTGGTAAGTAGATTTTTTTCCATGCAACCTTTAGCTTAAAGAGTAGATCTACATAACAATTTCATTTTGGTGCTATGTAAAATTAGTATAAACTAATTTGATTCATCCAATGAATATAATGGTGTACACTTTTTGCTTCACAAATTGCAGGTCCTGTTGAAGTGGTTGATGATGTAACTGGCGGGTTAAAACTTTTGTAG

mRNA sequence

ATGCAACATAAGCACAATCGTCATTCAACGCCTGGATTTCAATTGCTTGAATTAGAATTTGTTGGGGCAAGCTGCCTTGTTTTGGGATACTGTATTGGTTCGAAGTATCCTCCTCGCATCTTTATCAAAGCTAAATTAGCCAAAATCAACCAATCCAGCAGAAATGGGAAGAAGGATAAGCCTAAAGAGCCATTGGAGGTCGAAAATTTGGCTGACATACTCGATGACTTCAAGATGGTTTTAGTTGTGAGAAATGATTTGAAAATGGGAAAAGGAAAAATTGCTGCTCAATGCAGTCATGCAACTTTGGGTCTCTATAAGAAGCTCCATTATCGTGCACCAAAAGCGTTGAATAGGTGGGAAATGTGTGGACAACCCAAGGTGGTACTGAAGATAGAAAGTGAGGAAGACATGCTAGTCTTGCAAGAGAGGGCAAAATCATTGAAGCTACCAACCCATATTACCATTGATGCCGGAAAAACTCAGATTGCTCCTAATTCAAGAACGGTGATGGCAATTCTTGGTCCTGTTGAAGTGGTTGATGATGTAACTGGCGGGTTAAAACTTTTGTAG

Coding sequence (CDS)

ATGCAACATAAGCACAATCGTCATTCAACGCCTGGATTTCAATTGCTTGAATTAGAATTTGTTGGGGCAAGCTGCCTTGTTTTGGGATACTGTATTGGTTCGAAGTATCCTCCTCGCATCTTTATCAAAGCTAAATTAGCCAAAATCAACCAATCCAGCAGAAATGGGAAGAAGGATAAGCCTAAAGAGCCATTGGAGGTCGAAAATTTGGCTGACATACTCGATGACTTCAAGATGGTTTTAGTTGTGAGAAATGATTTGAAAATGGGAAAAGGAAAAATTGCTGCTCAATGCAGTCATGCAACTTTGGGTCTCTATAAGAAGCTCCATTATCGTGCACCAAAAGCGTTGAATAGGTGGGAAATGTGTGGACAACCCAAGGTGGTACTGAAGATAGAAAGTGAGGAAGACATGCTAGTCTTGCAAGAGAGGGCAAAATCATTGAAGCTACCAACCCATATTACCATTGATGCCGGAAAAACTCAGATTGCTCCTAATTCAAGAACGGTGATGGCAATTCTTGGTCCTGTTGAAGTGGTTGATGATGTAACTGGCGGGTTAAAACTTTTGTAG

Protein sequence

MQHKHNRHSTPGFQLLELEFVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKLL

Homology

[Close]

BLAST of Csor.00g028080 vs. ExPASy Swiss-Prot
Match: Q9Y3E5 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Homo sapiens OX=9606 GN=PTRH2 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.3e-30
Identity = 72/137 (52.55%), Postives = 92/137 (67.15%), Query Frame = 0


Query: 57  KKDKPKEPLEVENLADILDD---FKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRA 116
           K    K   + E+ A IL D   +KM+LVVRNDLKMGKGK+AAQCSHA +  YK++  R 
Sbjct: 42  KSKTSKTHTDTESEASILGDSGEYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRN 101

Query: 117 PKALNRWEMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAI 176
           P+ L +WE CGQPKVV+K   EE ++ L   AK L L   +  DAG+TQIAP S+TV+ I
Sbjct: 102 PEMLKQWEYCGQPKVVVKAPDEETLIALLAHAKMLGLTVSLIQDAGRTQIAPGSQTVLGI 161

Query: 177 -LGPVEVVDDVTGGLKL 190
             GP +++D VTG LKL
Sbjct: 162 GPGPADLIDKVTGHLKL 178

[Close]

BLAST of Csor.00g028080 vs. ExPASy Swiss-Prot
Match: Q3ZBL5 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Bos taurus OX=9913 GN=PTRH2 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 6.6e-30
Identity = 70/128 (54.69%), Postives = 88/128 (68.75%), Query Frame = 0


Query: 66  EVENLADIL---DDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEM 125
           + E  A IL    ++KM+LVVRNDLKMGKGK+AAQCSHA +  YK++  R P+ L  WE 
Sbjct: 51  DTETEASILGESGEYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRNPELLKEWEY 110

Query: 126 CGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAI-LGPVEVVD 185
           CGQPKVV+K   EE ++ L   AK L L   +  DAG+TQIAP SRTV+ I  GP +++D
Sbjct: 111 CGQPKVVVKAPDEETLVELLTHAKVLGLTVSLIQDAGRTQIAPGSRTVLGIGPGPADLID 170

Query: 186 DVTGGLKL 190
            VTG LKL
Sbjct: 171 KVTGHLKL 178

[Close]

BLAST of Csor.00g028080 vs. ExPASy Swiss-Prot
Match: Q8R2Y8 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Mus musculus OX=10090 GN=Ptrh2 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 6.6e-30
Identity = 65/115 (56.52%), Postives = 84/115 (73.04%), Query Frame = 0


Query: 76  DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIESE 135
           ++KM+LVVR DLKMGKGK+AAQCSHA +  YK+   R+P+ L  WE CGQPKVV+K   E
Sbjct: 66  EYKMILVVRTDLKMGKGKVAAQCSHAAVSAYKQTQRRSPQVLKEWEYCGQPKVVVKAPDE 125

Query: 136 EDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAI-LGPVEVVDDVTGGLKL 190
           + ++ L   AK+L L   +  DAG+TQI P SRTV+ I  GPVE++D+VTG LKL
Sbjct: 126 DTLIQLLTHAKTLGLTVSLIQDAGRTQIEPGSRTVLGIGPGPVELIDEVTGHLKL 180

[Close]

BLAST of Csor.00g028080 vs. ExPASy Swiss-Prot
Match: O97067 (Probable peptidyl-tRNA hydrolase 2 OS=Drosophila melanogaster OX=7227 GN=CG1307 PE=2 SV=2)

HSP 1 Score: 120.9 bits (302), Expect = 1.5e-26
Identity = 61/118 (51.69%), Postives = 84/118 (71.19%), Query Frame = 0


Query: 75  DDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIES 134
           D+FKMVLVVRNDLKMGKGKIAAQC H  +G Y++   R P+ L  WE CG  K+ +++ES
Sbjct: 70  DNFKMVLVVRNDLKMGKGKIAAQCGHGAVGAYQRAVVRTPRLLRSWENCGCAKIAVRVES 129

Query: 135 EEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEV--VDDVTGGLKLL 191
           E +++ +++ A+  +L T +  DAG+TQI  NS+TV+A+ GP     +D VTG LKLL
Sbjct: 130 EAELMAIKKEAERQQLNTCLIRDAGRTQIEANSKTVLAV-GPAAAADIDRVTGHLKLL 186

[Close]

BLAST of Csor.00g028080 vs. ExPASy Swiss-Prot
Match: O13830 (Probable peptidyl-tRNA hydrolase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC19A8.14 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 7.8e-23
Identity = 72/183 (39.34%), Postives = 98/183 (53.55%), Query Frame = 0


Query: 27  VLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKP---KEPLEVENLADILD-------- 86
           V+G+ IG KY      +   +K    + N +K+ P       + E+  ++LD        
Sbjct: 22  VVGFMIGRKYSVADASRGYSSKNANKASNPEKESPVSVSNDEDSESETELLDMLKGNSSL 81

Query: 87  --------DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPK 146
                     KMVLVVR DL M KGKIAAQC+HA L  YK      P  +  WE  GQ K
Sbjct: 82  AALALAEGQTKMVLVVRTDLGMTKGKIAAQCAHAALACYKIASAVDPDLVRIWENAGQAK 141

Query: 147 VVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAI-LGPVEVVDDVTGG 190
           + L+ ++EE + +LQ +A SL L   +  DAG+TQIA  S TV+ I  GPV V+++VTG 
Sbjct: 142 ITLQAQTEETLELLQAQAMSLGLCARVIHDAGRTQIASGSATVLGIGPGPVSVINEVTGS 201

[Close]

BLAST of Csor.00g028080 vs. NCBI nr
Match: KAG6571187.1 (Peptidyl-tRNA hydrolase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 379 bits (974), Expect = 1.03e-132
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = 0


Query: 1   MQHKHNRHSTPGFQLLELEFVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK 60
           MQHKHNRHSTPGFQLLELEFVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK
Sbjct: 1   MQHKHNRHSTPGFQLLELEFVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK 60

Query: 61  PKEPLEVENLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRW 120
           PKEPLEVENLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRW
Sbjct: 61  PKEPLEVENLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRW 120

Query: 121 EMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV 180
           EMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV
Sbjct: 121 EMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV 180

Query: 181 DDVTGGLKLL 190
           DDVTGGLKLL
Sbjct: 181 DDVTGGLKLL 190

[Close]

BLAST of Csor.00g028080 vs. NCBI nr
Match: XP_023511827.1 (peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 353 bits (905), Expect = 2.49e-122
Identity = 180/190 (94.74%), Postives = 181/190 (95.26%), Query Frame = 0


Query: 1   MQHKHNRHSTPGFQLLELEFVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK 60
           MQHKHNRHSTP         VGASCLVLGYCIGSKYPPRIFIKA+LAKINQSSRNGKKDK
Sbjct: 1   MQHKHNRHSTP---------VGASCLVLGYCIGSKYPPRIFIKARLAKINQSSRNGKKDK 60

Query: 61  PKEPLEVENLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRW 120
           PKEPLEVENLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRW
Sbjct: 61  PKEPLEVENLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRW 120

Query: 121 EMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV 180
           EMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV
Sbjct: 121 EMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV 180

Query: 181 DDVTGGLKLL 190
           DDVTGGLKLL
Sbjct: 181 DDVTGGLKLL 181

[Close]

BLAST of Csor.00g028080 vs. NCBI nr
Match: XP_022944764.1 (peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 352 bits (904), Expect = 3.54e-122
Identity = 180/190 (94.74%), Postives = 181/190 (95.26%), Query Frame = 0


Query: 1   MQHKHNRHSTPGFQLLELEFVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK 60
           MQHKHNRHSTP         VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK
Sbjct: 1   MQHKHNRHSTP---------VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK 60

Query: 61  PKEPLEVENLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRW 120
           PKEPLEVENLADILDDFKMVLVVRNDLK+GKGKIAAQCSHATLGLYKKLHYRAPKALNRW
Sbjct: 61  PKEPLEVENLADILDDFKMVLVVRNDLKIGKGKIAAQCSHATLGLYKKLHYRAPKALNRW 120

Query: 121 EMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV 180
           EMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV
Sbjct: 121 EMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV 180

Query: 181 DDVTGGLKLL 190
           DDVTGGLKLL
Sbjct: 181 DDVTGGLKLL 181

[Close]

BLAST of Csor.00g028080 vs. NCBI nr
Match: XP_022986616.1 (peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 350 bits (899), Expect = 2.05e-121
Identity = 179/190 (94.21%), Postives = 181/190 (95.26%), Query Frame = 0


Query: 1   MQHKHNRHSTPGFQLLELEFVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK 60
           MQHKHNRHSTP         VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK
Sbjct: 1   MQHKHNRHSTP---------VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK 60

Query: 61  PKEPLEVENLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRW 120
           PKEPLEVENLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLH+RAPKALNRW
Sbjct: 61  PKEPLEVENLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRW 120

Query: 121 EMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV 180
           EMCGQPKVVLKIESEED+LVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV
Sbjct: 121 EMCGQPKVVLKIESEEDILVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV 180

Query: 181 DDVTGGLKLL 190
           DDVTGGLKLL
Sbjct: 181 DDVTGGLKLL 181

[Close]

BLAST of Csor.00g028080 vs. NCBI nr
Match: KAG7010995.1 (Peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 336 bits (862), Expect = 8.66e-116
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 0


Query: 21  VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENLADILDDFKMV 80
           VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENLADILDDFKMV
Sbjct: 11  VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENLADILDDFKMV 70

Query: 81  LVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIESEEDMLV 140
           LVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIESEEDMLV
Sbjct: 71  LVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIESEEDMLV 130

Query: 141 LQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 190
           LQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKLL
Sbjct: 131 LQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 180

[Close]

BLAST of Csor.00g028080 vs. ExPASy TrEMBL
Match: A0A6J1FZ05 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111449121 PE=4 SV=1)

HSP 1 Score: 352 bits (904), Expect = 1.72e-122
Identity = 180/190 (94.74%), Postives = 181/190 (95.26%), Query Frame = 0


Query: 1   MQHKHNRHSTPGFQLLELEFVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK 60
           MQHKHNRHSTP         VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK
Sbjct: 1   MQHKHNRHSTP---------VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK 60

Query: 61  PKEPLEVENLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRW 120
           PKEPLEVENLADILDDFKMVLVVRNDLK+GKGKIAAQCSHATLGLYKKLHYRAPKALNRW
Sbjct: 61  PKEPLEVENLADILDDFKMVLVVRNDLKIGKGKIAAQCSHATLGLYKKLHYRAPKALNRW 120

Query: 121 EMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV 180
           EMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV
Sbjct: 121 EMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV 180

Query: 181 DDVTGGLKLL 190
           DDVTGGLKLL
Sbjct: 181 DDVTGGLKLL 181

[Close]

BLAST of Csor.00g028080 vs. ExPASy TrEMBL
Match: A0A6J1JBP2 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484304 PE=4 SV=1)

HSP 1 Score: 350 bits (899), Expect = 9.92e-122
Identity = 179/190 (94.21%), Postives = 181/190 (95.26%), Query Frame = 0


Query: 1   MQHKHNRHSTPGFQLLELEFVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK 60
           MQHKHNRHSTP         VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK
Sbjct: 1   MQHKHNRHSTP---------VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDK 60

Query: 61  PKEPLEVENLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRW 120
           PKEPLEVENLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLH+RAPKALNRW
Sbjct: 61  PKEPLEVENLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRW 120

Query: 121 EMCGQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV 180
           EMCGQPKVVLKIESEED+LVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV
Sbjct: 121 EMCGQPKVVLKIESEEDILVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVV 180

Query: 181 DDVTGGLKLL 190
           DDVTGGLKLL
Sbjct: 181 DDVTGGLKLL 181

[Close]

BLAST of Csor.00g028080 vs. ExPASy TrEMBL
Match: A0A6J1FZ04 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111449121 PE=4 SV=1)

HSP 1 Score: 335 bits (858), Expect = 1.71e-115
Identity = 169/170 (99.41%), Postives = 170/170 (100.00%), Query Frame = 0


Query: 21  VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENLADILDDFKMV 80
           VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENLADILDDFKMV
Sbjct: 11  VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENLADILDDFKMV 70

Query: 81  LVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIESEEDMLV 140
           LVVRNDLK+GKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIESEEDMLV
Sbjct: 71  LVVRNDLKIGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIESEEDMLV 130

Query: 141 LQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 190
           LQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKLL
Sbjct: 131 LQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 180

[Close]

BLAST of Csor.00g028080 vs. ExPASy TrEMBL
Match: A0A6J1J827 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484304 PE=4 SV=1)

HSP 1 Score: 333 bits (853), Expect = 9.53e-115
Identity = 168/170 (98.82%), Postives = 170/170 (100.00%), Query Frame = 0


Query: 21  VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENLADILDDFKMV 80
           VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENLADILDDFKMV
Sbjct: 10  VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENLADILDDFKMV 69

Query: 81  LVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIESEEDMLV 140
           LVVRNDLKMGKGKIAAQCSHATLGLYKKLH+RAPKALNRWEMCGQPKVVLKIESEED+LV
Sbjct: 70  LVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCGQPKVVLKIESEEDILV 129

Query: 141 LQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 190
           LQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKLL
Sbjct: 130 LQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 179

[Close]

BLAST of Csor.00g028080 vs. ExPASy TrEMBL
Match: A0A6J1G686 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111451148 PE=4 SV=1)

HSP 1 Score: 321 bits (823), Expect = 3.69e-110
Identity = 162/170 (95.29%), Postives = 165/170 (97.06%), Query Frame = 0


Query: 21  VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENLADILDDFKMV 80
           VGA CLVLGYCIGSKYPPRIFIKAKLAK N+SSR GKK KPKEPLEVENLADIL+DFKMV
Sbjct: 11  VGAGCLVLGYCIGSKYPPRIFIKAKLAKSNESSRTGKKGKPKEPLEVENLADILEDFKMV 70

Query: 81  LVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIESEEDMLV 140
           LVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMC QPKVVLKIESEEDMLV
Sbjct: 71  LVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVLKIESEEDMLV 130

Query: 141 LQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 190
           LQERAKSLKLPTHITIDAG+TQIAPNSRTVMAILGPVEVVDDVTGGLKLL
Sbjct: 131 LQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 180

[Close]

BLAST of Csor.00g028080 vs. TAIR 10
Match: AT3G03010.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 243.4 bits (620), Expect = 1.4e-64
Identity = 130/177 (73.45%), Postives = 145/177 (81.92%), Query Frame = 0


Query: 15  LLELEFVGASCLVLGYCIGS-KYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENLADI 74
           LL +  VGA+   LGY I + +   RIF+ +K   I+  S   KK K KEPLE+E LAD 
Sbjct: 6   LLSVLLVGAA---LGYYISTLRQTRRIFLSSKSVAIDAGSSGNKKTKSKEPLEIEKLADF 65

Query: 75  LDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIE 134
             +FKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKL  RAPKALNRWE C QPKVV+KIE
Sbjct: 66  RKNFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLLQRAPKALNRWEYCAQPKVVVKIE 125

Query: 135 SEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 191
           SEE+MLVLQERAK+LKLPTHITIDAGKTQIAPNSRTVMAILGPV+ VD+VTGGLKL+
Sbjct: 126 SEEEMLVLQERAKTLKLPTHITIDAGKTQIAPNSRTVMAILGPVDNVDEVTGGLKLM 179

[Close]

BLAST of Csor.00g028080 vs. TAIR 10
Match: AT3G03010.2 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 243.4 bits (620), Expect = 1.4e-64
Identity = 130/177 (73.45%), Postives = 145/177 (81.92%), Query Frame = 0


Query: 15  LLELEFVGASCLVLGYCIGS-KYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENLADI 74
           LL +  VGA+   LGY I + +   RIF+ +K   I+  S   KK K KEPLE+E LAD 
Sbjct: 6   LLSVLLVGAA---LGYYISTLRQTRRIFLSSKSVAIDAGSSGNKKTKSKEPLEIEKLADF 65

Query: 75  LDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIE 134
             +FKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKL  RAPKALNRWE C QPKVV+KIE
Sbjct: 66  RKNFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLLQRAPKALNRWEYCAQPKVVVKIE 125

Query: 135 SEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 191
           SEE+MLVLQERAK+LKLPTHITIDAGKTQIAPNSRTVMAILGPV+ VD+VTGGLKL+
Sbjct: 126 SEEEMLVLQERAKTLKLPTHITIDAGKTQIAPNSRTVMAILGPVDNVDEVTGGLKLM 179

[Close]

BLAST of Csor.00g028080 vs. TAIR 10
Match: AT5G16870.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 229.6 bits (584), Expect = 2.2e-60
Identity = 125/170 (73.53%), Postives = 140/170 (82.35%), Query Frame = 0


Query: 21  VGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENLADILDDFKMV 80
           VGA+   LGYCIG++       K  +A ++   ++ K    K PLE+E LADILDDFKMV
Sbjct: 11  VGAA---LGYCIGTRRS-----KPVVAAVD---KDIKTQSSKSPLEIEKLADILDDFKMV 70

Query: 81  LVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIESEEDMLV 140
           LVVRNDLKMGKGKIAAQCSHATLGLYKKL  RAPKAL+ WE C QPKVV+KIE E++ML 
Sbjct: 71  LVVRNDLKMGKGKIAAQCSHATLGLYKKLVRRAPKALDCWEECAQPKVVVKIEDEDEMLE 130

Query: 141 LQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 191
           LQERAKSLKLPTHITIDAG+TQIAPNSRTVMAILGPVEVVD+VTGGLKLL
Sbjct: 131 LQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDEVTGGLKLL 169

[Close]

BLAST of Csor.00g028080 vs. TAIR 10
Match: AT4G32900.2 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 113.2 bits (282), Expect = 2.3e-25
Identity = 58/116 (50.00%), Postives = 81/116 (69.83%), Query Frame = 0


Query: 76  DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIESE 135
           + KMVLVVR DLKM  GKIA+QC+HA  G+Y +L       L RWE  GQPK+V+  +++
Sbjct: 78  ELKMVLVVRQDLKMRTGKIASQCAHAATGMYAELMQSDRYLLRRWEENGQPKIVVTCKNQ 137

Query: 136 EDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAI-LGPVEVVDDVTGGLKLL 191
           ++M  + E A+S+ LPT +  DAG+T++A  SRTV+A+  GP E+VD +TG L LL
Sbjct: 138 QEMNKITEAAESVGLPTFVVADAGRTEVAAGSRTVLAVGPGPKELVDSITGRLALL 193

[Close]

BLAST of Csor.00g028080 vs. TAIR 10
Match: AT4G32900.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 68.6 bits (166), Expect = 6.4e-12
Identity = 32/63 (50.79%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 76  DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVVLKIESE 135
           + KMVLVVR DLKM  GKIA+QC+HA  G+Y +L       L RWE  GQPK+V+  +++
Sbjct: 78  ELKMVLVVRQDLKMRTGKIASQCAHAATGMYAELMQSDRYLLRRWEENGQPKIVVTCKNQ 137

Query: 136 EDM 139
           ++M
Sbjct: 138 QEM 140

The following BLAST results are available for this feature:

BLAST of Csor.00g028080 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9Y3E5	1.3e-30	52.55	Peptidyl-tRNA hydrolase 2, mitochondrial OS=Homo sapiens OX=9606 GN=PTRH2 PE=1 S...	[more]
Q3ZBL5	6.6e-30	54.69	Peptidyl-tRNA hydrolase 2, mitochondrial OS=Bos taurus OX=9913 GN=PTRH2 PE=2 SV=...	[more]
Q8R2Y8	6.6e-30	56.52	Peptidyl-tRNA hydrolase 2, mitochondrial OS=Mus musculus OX=10090 GN=Ptrh2 PE=1 ...	[more]
O97067	1.5e-26	51.69	Probable peptidyl-tRNA hydrolase 2 OS=Drosophila melanogaster OX=7227 GN=CG1307 ...	[more]
O13830	7.8e-23	39.34	Probable peptidyl-tRNA hydrolase 2 OS=Schizosaccharomyces pombe (strain 972 / AT...	[more]

BLAST of Csor.00g028080 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571187.1	1.03e-132	100.00	Peptidyl-tRNA hydrolase 2, mitochondrial, partial [Cucurbita argyrosperma subsp....	[more]
XP_023511827.1	2.49e-122	94.74	peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X1 [Cucurbita pepo subsp. ...	[more]
XP_022944764.1	3.54e-122	94.74	peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X1 [Cucurbita moschata]	[more]
XP_022986616.1	2.05e-121	94.21	peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X1 [Cucurbita maxima]	[more]
KAG7010995.1	8.66e-116	100.00	Peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosp...	[more]

BLAST of Csor.00g028080 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FZ05	1.72e-122	94.74	Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111449121 PE=4 SV=1	[more]
A0A6J1JBP2	9.92e-122	94.21	Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484304 PE=4 SV=1	[more]
A0A6J1FZ04	1.71e-115	99.41	Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111449121 PE=4 SV=1	[more]
A0A6J1J827	9.53e-115	98.82	Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484304 PE=4 SV=1	[more]
A0A6J1G686	3.69e-110	95.29	Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111451148 PE=4 SV=1	[more]

BLAST of Csor.00g028080 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G03010.1	1.4e-64	73.45	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT3G03010.2	1.4e-64	73.45	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT5G16870.1	2.2e-60	73.53	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT4G32900.2	2.3e-25	50.00	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT4G32900.1	6.4e-12	50.79	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002833	Peptidyl-tRNA hydrolase, PTH2	PFAM	PF01981	PTH2	coord: 78..190 e-value: 1.9E-41 score: 141.1
IPR002833	Peptidyl-tRNA hydrolase, PTH2	TIGRFAM	TIGR00283	TIGR00283	coord: 78..190 e-value: 2.1E-34 score: 116.3
IPR002833	Peptidyl-tRNA hydrolase, PTH2	PANTHER	PTHR12649	PEPTIDYL-TRNA HYDROLASE 2	coord: 16..190
IPR002833	Peptidyl-tRNA hydrolase, PTH2	CDD	cd02430	PTH2	coord: 77..190 e-value: 9.22421E-64 score: 190.427
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	GENE3D	3.40.1490.10	Bit1	coord: 75..190 e-value: 1.3E-44 score: 153.0
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	SUPERFAMILY	102462	Peptidyl-tRNA hydrolase II	coord: 75..190
None	No IPR available	PANTHER	PTHR12649:SF19	OSJNBA0060D06.11 PROTEIN	coord: 16..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g028080.m01	Csor.00g028080.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005829	cytosol
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004045	aminoacyl-tRNA hydrolase activity