Csor.00g027350 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g027350
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptioncucumisin-like
LocationCsor_Chr04: 5968982 .. 5971747 (+)
RNA-Seq ExpressionCsor.00g027350
SyntenyCsor.00g027350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATAAGAAAAGGCAGTAATATGAGTTCTCCTCTGATCCTTAATCTCCTCTTCCTTGCCTTGTTCTGCAGTGTGCTGGCATCCACCTCCCATTCTCACGATGATGATAGGAAGATATATATTGTGTACATGGGGAGCAAGCTACAGGACCCTTCTTCCGCTCATTTGCATCACCGAGCAATGTTGGAGCAAGTGGTTGGCAGGTTTATAACACACTCTTCATCAACAATTATTTGAACAAATTATTTGAACAAATTATTTGAACAAATTATTTGAAATTTTTTTGTATGTTGTTTCAGCACTTTTGATCCACAATCTGTGTTATACACCTACAATAGAAGTTTCAACGGATTCGTGGTGAGACTCACTGAAGAAGAAGCTCAAGAGATTGCTAGTACGTACGTGTATCTTTTATTTTTATTTCAAGTTATTTTTGAAATTTGAAATTTGAAATTTGAAATTTGAAATGTTTTTCCAGGTAAGGAGGGTGTGGTCTCTGTGTTCCTCAATGAAAACAACCATCTTCACACCACAAGATCATGGGATTTTCTGGGTTTCTCACAAAATGTTAGTCGCGTAAACCAAGTGGAAAGCAACATAGTTGTTGGAGTTATGGACAGCGGAATCTGGCCAGAGTCTCCCAGTTTCAACGATGAAGCCTTTGGTCCTGCACCACCCAACTGGAAGGGCATATGCCAAAACAACAACAACAACAACTTCAGTTGCAACAGGTAAACGATTATATTCCAATTACAGACAAAATTTATAAATCTATGACAACTAAACATGCGAATGCTTGGAACTTTGTTAGAAAAATCATCGGAGCTCGAGCATACCACATTGGGAAGTCCATTGCCCCTGACGAAGTTAATAGTCCAAGAGATACCAACGGCCATGGGACCCACACAGCGTCCATAGTGGCTGGCGGTCTGGTGAGCCAGGCAAGTTTGTATAATCTCGGCCTCGGCACGGCCAGAGGAGGGGCTCCCTCTGCGCGGATTGCGGTGTATAAAATATGTTGGAAACATGGGTGCTCCGACGCCGACATTCTTGCGGCATTCGACGATGCAATTGCTGATGGGGTCGATTTCATTTCCCTTTCCGTTGGCTCCAGTAAACCGCGCCCTTATTTCAGCGATCCCATTGCTATTGGATCTTTCCATGCAATGAAGAAAGGAATATTGACCTCCAATTCCGCCGGAAACGAAGGTCCCAAGGACTCCACCACCACAAGCTTGTCTCCATGGCTTCTTTCTGTCGCTGCAACCACCATGGACAGAAAATTTGTCACTCGAGTAAGGCTTGGCGATGGAAGATTCTTCCAGGTAACATTATTTTTTAATTTATTTTTTTTGTCACCTGATATGCTATGACTGGTTTTATATATGTTATTATATAAATCATATATATAATTATATATATATAACATATAAAATTGTAATGTTTTCATGAAAGTGCAGGGAGTTTCAATCAACACGTTCAACACTATGGTTTATCAATATCCCTTAGTTTCTCTTCACTAAGTTCAACGCTATAGTTCCTCCTTCTCTATTCTCTTCGTTGGGTGCCATAAATGGCGTCATAATGCAATCTGGTACAAGGGATAATGCAACTTCCTATCCCCTGCCTGCTGCCCTCGTCAGCCGACAAGATGTCATTACCATTTTTCGCTTCACTCGTGAAACACTGTATGTTTCTCTTCACTTTCTTCGATTCAATTTAACAGGTTTATTGAAATAATTTATTATTTCAATTAACAGCACGCCGTATGCCATGTTTTTCAAGAGTAACGCTACACAAGATCCATCTGCTCCTGTCATTGCTTCCTTCTCATCTCGGGGACCTAATTCTCTAACCAACCACATTCTCAAGGTTTTTCCTCCTTTCCAATTCAAACAATAAATTGCGTTTGTCTTTCCATCATTCATTTGTTTCATTTACTTCCACACATGCAGCCAGATTTGAGTGGTCCTGGAGTTGAAATTCTAGGAGCATGGCCTCCCGTTGCGCCAATAGCTGAAGTTAAAGACTCCAGGAGTTCGCTTTACAATATAATCTCAGGGACCTCCATGTCTTGCTCACATATCACTGGAGTCGCAGTGTATGTTAAAACATTCAATCCAACCTTGTCTCCCGCTGCTATAAAATCGGCACTCATGACAACCGGTAATTATTACTAACTGCTCTGTTTTTCTAAATTTTGTGTGCTTTTCACTCACTCCTCTCTCTTTCTACACAGCTTCGCCCATGAATGCTAGCCTCCACTCACAGGCGGAGTTTGCGTATGGCGCAGGCCATGTCAACCCACTGAAGGCTGTAAATCCAGGGTTGGTGTACGATGCAGATGAAAATGAGTATGTGAAATTCTTGTGTGGTCAAGGTTACGACACGGCCATGGTTCGACGTATCACTGGGGATAATAGTGCTTGCACTTCTGGCAACATTGGAAGAGTATGGGATCTAAACCTTCCTTCTTTTGCACTTTCGGTAGCGGCCGGTTCGAAAGCCCTCAATCAGTACTTCACTAGAACTCTCACAAATGTGGCATCCAGTGGATCCACATATAGAGCTACCATTTCTGCCCCAAAAGGCCTTCGCATCACAGTGAATCCCTCTGTTCTATCATTTAATCGCATTGGGGAAAGGAAATCCTTCAATTTGACAGTTGGTGGAACAGTGAGTCGTAGGTTAGTCTCTGCTTGTTTGGTGTGGAGTGATGGTGTTCATTCCGTCCGAAGCCCTATTACAATCTCCACTTCCCGTTGA

mRNA sequence

ATGATAAGAAAAGGCAGTAATATGAGTTCTCCTCTGATCCTTAATCTCCTCTTCCTTGCCTTGTTCTGCAGTGTGCTGGCATCCACCTCCCATTCTCACGATGATGATAGGAAGATATATATTGTGTACATGGGGAGCAAGCTACAGGACCCTTCTTCCGCTCATTTGCATCACCGAGCAATGTTGGAGCAAGTGGTTGGCAGCACTTTTGATCCACAATCTGTGTTATACACCTACAATAGAAGTTTCAACGGATTCGTGGTGAGACTCACTGAAGAAGAAGCTCAAGAGATTGCTAGTAAGGAGGGTGTGGTCTCTGTGTTCCTCAATGAAAACAACCATCTTCACACCACAAGATCATGGGATTTTCTGGGTTTCTCACAAAATGTTAGTCGCGTAAACCAAGTGGAAAGCAACATAGTTGTTGGAGTTATGGACAGCGGAATCTGGCCAGAGTCTCCCAGTTTCAACGATGAAGCCTTTGGTCCTGCACCACCCAACTGGAAGGGCATATGCCAAAACAACAACAACAACAACTTCAGTTGCAACAGAAAAATCATCGGAGCTCGAGCATACCACATTGGGAAGTCCATTGCCCCTGACGAAGTTAATAGTCCAAGAGATACCAACGGCCATGGGACCCACACAGCGTCCATAGTGGCTGGCGGTCTGGTGAGCCAGGCAAGTTTGTATAATCTCGGCCTCGGCACGGCCAGAGGAGGGGCTCCCTCTGCGCGGATTGCGGTGTATAAAATATGTTGGAAACATGGGTGCTCCGACGCCGACATTCTTGCGGCATTCGACGATGCAATTGCTGATGGGGTCGATTTCATTTCCCTTTCCGTTGGCTCCAGTAAACCGCGCCCTTATTTCAGCGATCCCATTGCTATTGGATCTTTCCATGCAATGAAGAAAGGAATATTGACCTCCAATTCCGCCGGAAACGAAGGTCCCAAGGACTCCACCACCACAAGCTTGTCTCCATGGCTTCTTTCTGTCGCTGCAACCACCATGGACAGAAAATTTGTCACTCGAGTAAGGCTTGGCGATGGAAGATTCTTCCAGTTTCTCTTCACTAAGTTCAACGCTATAGTTCCTCCTTCTCTATTCTCTTCGTTGGGTGCCATAAATGGCGTCATAATGCAATCTGGTACAAGGGATAATGCAACTTCCTATCCCCTGCCTGCTGCCCTCGTCAGCCGACAAGATGTCATTACCATTTTTCGCTTCACTCGTGAAACACTCACGCCGTATGCCATGTTTTTCAAGAGTAACGCTACACAAGATCCATCTGCTCCTGTCATTGCTTCCTTCTCATCTCGGGGACCTAATTCTCTAACCAACCACATTCTCAAGCCAGATTTGAGTGGTCCTGGAGTTGAAATTCTAGGAGCATGGCCTCCCGTTGCGCCAATAGCTGAAGTTAAAGACTCCAGGAGTTCGCTTTACAATATAATCTCAGGGACCTCCATGTCTTGCTCACATATCACTGGAGTCGCAGTGTATGTTAAAACATTCAATCCAACCTTGTCTCCCGCTGCTATAAAATCGGCACTCATGACAACCGCTTCGCCCATGAATGCTAGCCTCCACTCACAGGCGGAGTTTGCGTATGGCGCAGGCCATGTCAACCCACTGAAGGCTGTAAATCCAGGGTTGGTGTACGATGCAGATGAAAATGAGTATGTGAAATTCTTGTGTGGTCAAGGTTACGACACGGCCATGGTTCGACGTATCACTGGGGATAATAGTGCTTGCACTTCTGGCAACATTGGAAGAGTATGGGATCTAAACCTTCCTTCTTTTGCACTTTCGGTAGCGGCCGGTTCGAAAGCCCTCAATCAGTACTTCACTAGAACTCTCACAAATGTGGCATCCAGTGGATCCACATATAGAGCTACCATTTCTGCCCCAAAAGGCCTTCGCATCACAGTGAATCCCTCTGTTCTATCATTTAATCGCATTGGGGAAAGGAAATCCTTCAATTTGACAGTTGGTGGAACAGTGAGTCGTAGGTTAGTCTCTGCTTGTTTGGTGTGGAGTGATGGTGTTCATTCCGTCCGAAGCCCTATTACAATCTCCACTTCCCGTTGA

Coding sequence (CDS)

ATGATAAGAAAAGGCAGTAATATGAGTTCTCCTCTGATCCTTAATCTCCTCTTCCTTGCCTTGTTCTGCAGTGTGCTGGCATCCACCTCCCATTCTCACGATGATGATAGGAAGATATATATTGTGTACATGGGGAGCAAGCTACAGGACCCTTCTTCCGCTCATTTGCATCACCGAGCAATGTTGGAGCAAGTGGTTGGCAGCACTTTTGATCCACAATCTGTGTTATACACCTACAATAGAAGTTTCAACGGATTCGTGGTGAGACTCACTGAAGAAGAAGCTCAAGAGATTGCTAGTAAGGAGGGTGTGGTCTCTGTGTTCCTCAATGAAAACAACCATCTTCACACCACAAGATCATGGGATTTTCTGGGTTTCTCACAAAATGTTAGTCGCGTAAACCAAGTGGAAAGCAACATAGTTGTTGGAGTTATGGACAGCGGAATCTGGCCAGAGTCTCCCAGTTTCAACGATGAAGCCTTTGGTCCTGCACCACCCAACTGGAAGGGCATATGCCAAAACAACAACAACAACAACTTCAGTTGCAACAGAAAAATCATCGGAGCTCGAGCATACCACATTGGGAAGTCCATTGCCCCTGACGAAGTTAATAGTCCAAGAGATACCAACGGCCATGGGACCCACACAGCGTCCATAGTGGCTGGCGGTCTGGTGAGCCAGGCAAGTTTGTATAATCTCGGCCTCGGCACGGCCAGAGGAGGGGCTCCCTCTGCGCGGATTGCGGTGTATAAAATATGTTGGAAACATGGGTGCTCCGACGCCGACATTCTTGCGGCATTCGACGATGCAATTGCTGATGGGGTCGATTTCATTTCCCTTTCCGTTGGCTCCAGTAAACCGCGCCCTTATTTCAGCGATCCCATTGCTATTGGATCTTTCCATGCAATGAAGAAAGGAATATTGACCTCCAATTCCGCCGGAAACGAAGGTCCCAAGGACTCCACCACCACAAGCTTGTCTCCATGGCTTCTTTCTGTCGCTGCAACCACCATGGACAGAAAATTTGTCACTCGAGTAAGGCTTGGCGATGGAAGATTCTTCCAGTTTCTCTTCACTAAGTTCAACGCTATAGTTCCTCCTTCTCTATTCTCTTCGTTGGGTGCCATAAATGGCGTCATAATGCAATCTGGTACAAGGGATAATGCAACTTCCTATCCCCTGCCTGCTGCCCTCGTCAGCCGACAAGATGTCATTACCATTTTTCGCTTCACTCGTGAAACACTCACGCCGTATGCCATGTTTTTCAAGAGTAACGCTACACAAGATCCATCTGCTCCTGTCATTGCTTCCTTCTCATCTCGGGGACCTAATTCTCTAACCAACCACATTCTCAAGCCAGATTTGAGTGGTCCTGGAGTTGAAATTCTAGGAGCATGGCCTCCCGTTGCGCCAATAGCTGAAGTTAAAGACTCCAGGAGTTCGCTTTACAATATAATCTCAGGGACCTCCATGTCTTGCTCACATATCACTGGAGTCGCAGTGTATGTTAAAACATTCAATCCAACCTTGTCTCCCGCTGCTATAAAATCGGCACTCATGACAACCGCTTCGCCCATGAATGCTAGCCTCCACTCACAGGCGGAGTTTGCGTATGGCGCAGGCCATGTCAACCCACTGAAGGCTGTAAATCCAGGGTTGGTGTACGATGCAGATGAAAATGAGTATGTGAAATTCTTGTGTGGTCAAGGTTACGACACGGCCATGGTTCGACGTATCACTGGGGATAATAGTGCTTGCACTTCTGGCAACATTGGAAGAGTATGGGATCTAAACCTTCCTTCTTTTGCACTTTCGGTAGCGGCCGGTTCGAAAGCCCTCAATCAGTACTTCACTAGAACTCTCACAAATGTGGCATCCAGTGGATCCACATATAGAGCTACCATTTCTGCCCCAAAAGGCCTTCGCATCACAGTGAATCCCTCTGTTCTATCATTTAATCGCATTGGGGAAAGGAAATCCTTCAATTTGACAGTTGGTGGAACAGTGAGTCGTAGGTTAGTCTCTGCTTGTTTGGTGTGGAGTGATGGTGTTCATTCCGTCCGAAGCCCTATTACAATCTCCACTTCCCGTTGA

Protein sequence

MIRKGSNMSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFLGFSQNVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSCNRKIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFLFTKFNAIVPPSLFSSLGAINGVIMQSGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNVASSGSTYRATISAPKGLRITVNPSVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWSDGVHSVRSPITISTSR
Homology
BLAST of Csor.00g027350 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 1.3e-264
Identity = 476/734 (64.85%), Postives = 550/734 (74.93%), Query Frame = 0

Query: 8   MSSPLILNLLFLALFCS-VLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVV 67
           MSS LI  L F +LF S  LAS   S DD + IYIVYMG KL+DP SAHLHHRAMLEQVV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 68  GSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFLGF 127
           GSTF P+SVL+TY RSFNGF V+LTEEEA++IAS EGVVSVFLNE N LHTTRSWDFLGF
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 128 SQNVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSCNRKI 187
              V R +QVESNIVVGV+D+GIWPESPSF+DE F P PP WKG C+   +NNF CNRKI
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCE--TSNNFRCNRKI 180

Query: 188 IGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSAR 247
           IGAR+YHIG+ I+P +VN PRDTNGHGTHTAS  AGGLVSQA+LY LGLGTARGG P AR
Sbjct: 181 IGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLAR 240

Query: 248 IAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKG 307
           IA YK+CW  GCSD DILAA+DDAIADGVD ISLSVG + PR YF D IAIGSFHA+++G
Sbjct: 241 IAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERG 300

Query: 308 ILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFL--------- 367
           ILTSNSAGN GP   TT SLSPWLLSVAA+TMDRKFVT+V++G+G+ FQ +         
Sbjct: 301 ILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQY 360

Query: 368 ------------------------------FTKFNAIV------PPSLFSSLGAINGVIM 427
                                           K   +V      P   F SL    GV+M
Sbjct: 361 YPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLM 420

Query: 428 QSGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSR 487
            S TRD A SYPLP++++   D++   R+     +P A  FKS    + SAPV+ SFSSR
Sbjct: 421 TSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 480

Query: 488 GPNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAV 547
           GPN  T  ++KPD+SGPGVEIL AWP VAP+  ++  R++L+NIISGTSMSC HITG+A 
Sbjct: 481 GPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIR--RNTLFNIISGTSMSCPHITGIAT 540

Query: 548 YVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENE 607
           YVKT+NPT SPAAIKSALMTTASPMNA  + QAEFAYG+GHVNPLKAV PGLVYDA+E++
Sbjct: 541 YVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESD 600

Query: 608 YVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTL 667
           YVKFLCGQGY+T  VRRITGD SACTSGN GRVWDLN PSF LSV+  S+  NQYF RTL
Sbjct: 601 YVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSP-SQTFNQYFNRTL 660

Query: 668 TNVASSGSTYRATISAPKGLRITVNPSVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWS 696
           T+VA   STYRA ISAP+GL I+VNP+VLSFN +G+RKSF LTV G++   +VSA LVWS
Sbjct: 661 TSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWS 720

BLAST of Csor.00g027350 vs. ExPASy Swiss-Prot
Match: Q9FIM5 (Subtilisin-like protease SBT4.9 OS=Arabidopsis thaliana OX=3702 GN=SBT4.9 PE=2 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 4.0e-157
Identity = 311/719 (43.25%), Postives = 444/719 (61.75%), Query Frame = 0

Query: 13  ILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSS-----AHLHHRAMLEQVVG 72
           +++ + ++   SV A T  S   D+++Y+VYMGS    PSS        HH ++L++V G
Sbjct: 10  LISCVLVSFVISVSAVTDDS--QDKQVYVVYMGSL---PSSRLEYTPMSHHMSILQEVTG 69

Query: 73  STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFLGFS 132
            +     ++ +Y RSFNGF  RLTE E + +A  EGVVSVF + N  L TT SWDFLG  
Sbjct: 70  ESSVEGRLVRSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTASWDFLGLK 129

Query: 133 Q--NVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSCNRK 192
           +  N  R   +ES+ ++G +DSGIWPES SF+D+ FGP P  WKG+C  +   NF+CN K
Sbjct: 130 EGKNTKRNLAIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVC--SAGKNFTCNNK 189

Query: 193 IIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSA 252
           +IGAR Y              RD  GHGTHTAS  AG  V   S Y +G GTARGG P++
Sbjct: 190 LIGARDY---------TNEGTRDIEGHGTHTASTAAGNAVKNTSFYGIGNGTARGGVPAS 249

Query: 253 RIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKK 312
           RIA YK C + GC+   +L+AFDDAIADGVD IS+S+G++  R Y +DPIAIG+FHAM K
Sbjct: 250 RIAAYKACSEMGCTTESVLSAFDDAIADGVDLISISLGANLVRTYETDPIAIGAFHAMVK 309

Query: 313 GILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFLFTKFNA-- 372
           GILT  SAGN GP   +  S++PW+L+VAA+  +R FVT+V LG+G+   F+    NA  
Sbjct: 310 GILTVQSAGNGGPNPGSVMSVAPWILTVAASNTNRGFVTKVVLGNGK--TFVGKSLNAFD 369

Query: 373 ----------------IVPPSLFSSLGAINGVIMQSGTRDNATSYP----LPAALVSRQD 432
                           ++   +  S   ++  I+ +   +N   Y     LP++ +S+ D
Sbjct: 370 LKGKNYPLYGGSTDGPLLRGKILVSEDKVSSEIVVANINENYHDYAYVSILPSSALSKDD 429

Query: 433 VITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPNSLTNHILKPDLSGPGVEIL 492
             ++  +   T +P+    KS A  + +AP +A FSSRGPN++   ILKPD++ PGVEIL
Sbjct: 430 FDSVISYVNSTKSPHGTVLKSEAIFNQAAPKVAGFSSRGPNTIAVDILKPDVTAPGVEIL 489

Query: 493 GAWPPV-APIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVKTFNPTLSPAAIKSALMTT 552
            A+ P+ +P  + +D+R   Y+++SGTSMSC H+ GVA Y+KTF+P  SP+ I+SA+MTT
Sbjct: 490 AAFSPLNSPAQDKRDNRHVKYSVLSGTSMSCPHVAGVAAYIKTFHPEWSPSMIQSAIMTT 549

Query: 553 ASPMNA--SLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVKFLCGQGYDTAMVRRIT 612
           A PMNA  +  +  EFAYGAGHV+P+ A+NPGLVY+  +++++ FLCG  Y+   ++ I 
Sbjct: 550 AWPMNATGTAVASTEFAYGAGHVDPIAAINPGLVYEIGKSDHIAFLCGLNYNATSLKLIA 609

Query: 613 GDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNVASSGSTYRATISAPKG 672
           G+   CT   + R  +LN PS +  +     +    F RT+TNV +  STY++ I    G
Sbjct: 610 GEAVTCTGKTLPR--NLNYPSMSAKLPKSESSFIVTFNRTVTNVGTPNSTYKSKIVLNHG 669

Query: 673 --LRITVNPSVLSFNRIGERKSFNLTVGGT-VSRRL-VSACLVWSDGVHSVRSPITIST 696
             L++ V+PSVLS   + E++SF +TV G+ +  +L  SA L+WSDG H+VRSPI + T
Sbjct: 670 SNLKVEVSPSVLSMKSVKEKQSFTVTVSGSNIDPKLPSSANLIWSDGTHNVRSPIVVYT 708

BLAST of Csor.00g027350 vs. ExPASy Swiss-Prot
Match: Q9FIM6 (Subtilisin-like protease SBT4.8 OS=Arabidopsis thaliana OX=3702 GN=SBT4.8 PE=3 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 1.4e-154
Identity = 301/708 (42.51%), Postives = 439/708 (62.01%), Query Frame = 0

Query: 19  LALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHL-HHRAMLEQVVGSTFDPQSVLY 78
           + LF S +++  +    D+++Y+VYMGS    P+   + +H  +L++V G +     ++ 
Sbjct: 14  IILFLSSVSAIIYD-PQDKQVYVVYMGSLPSQPNYTPMSNHINILQEVTGESSIEGRLVR 73

Query: 79  TYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFLGFSQ--NVSRVNQ 138
           +Y RSFNGF   LTE E + +A  EGVVSVF ++N  L TT SWDF+G  +  N  R   
Sbjct: 74  SYKRSFNGFSALLTESEREGVAEMEGVVSVFRSKNYKLQTTASWDFMGMKEGKNTKRNFA 133

Query: 139 VESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSCNRKIIGARAYHIG 198
           VES+ ++G +DSGIWPES SF+D+ FGP P  WKG+C+     NF+CN K+IGAR Y   
Sbjct: 134 VESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCK--GGKNFTCNNKLIGARDY--- 193

Query: 199 KSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVYKICWK 258
                      RD  GHGTHT S  AG  V+  S + +G GTARGG P++R+A YK+C  
Sbjct: 194 ------TSEGTRDLQGHGTHTTSTAAGNAVADTSFFGIGNGTARGGVPASRVAAYKVCTI 253

Query: 259 HGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTSNSAGN 318
            GCSD ++L+AFDDAIADGVD IS+S+G   P  Y  D IAIG+FHAM KGILT +SAGN
Sbjct: 254 TGCSDDNVLSAFDDAIADGVDLISVSLGGDYPSLYAEDTIAIGAFHAMAKGILTVHSAGN 313

Query: 319 EGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGR----------------------- 378
            GP  +T  S++PW+L+VAATT +R+F+T+V LG+G+                       
Sbjct: 314 AGPNPTTVVSVAPWMLTVAATTTNRRFLTKVVLGNGKTLVGKSVNAFDLKGKKYPLEYGD 373

Query: 379 FFQFLFTKFNAIVPPSLFSSLGAINGVIMQSGTRDNATSYPLPAALVSRQDVITIFRFTR 438
           +      K   +V   L  S  A++   + +  +D A+    P +++S+ D  ++  +  
Sbjct: 374 YLNESLVKGKILVSRYLSGSEVAVS--FITTDNKDYASISSRPLSVLSQDDFDSLVSYIN 433

Query: 439 ETLTPYAMFFKSNATQDPSAPVIASFSSRGPNSLTNHILKPDLSGPGVEILGAWPPVA-P 498
            T +P     K+ A  +  +P +ASFSSRGPN++   ILKPD+S PGVEIL A+ P++ P
Sbjct: 434 STRSPQGSVLKTEAIFNQLSPKVASFSSRGPNTIAVDILKPDISAPGVEILAAYSPLSLP 493

Query: 499 IAEVKDSRSSLYNIISGTSMSCSHITGVAVYVKTFNPTLSPAAIKSALMTTASPMNA--S 558
             + +D R   Y+++SGTSM+C H+TGVA Y+KTF+P  SP+ I+SA+MTTA  MNA  +
Sbjct: 494 SEDRRDKRRVKYSVLSGTSMACPHVTGVAAYIKTFHPDWSPSVIQSAIMTTAWQMNATGT 553

Query: 559 LHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVKFLCGQGYDTAMVRRITGDNSACTSG 618
                EFAYGAGHV+P+ A+NPGLVY+ ++ +++ FLCG  Y +  ++ I+GD   C+  
Sbjct: 554 GAESTEFAYGAGHVDPIAAINPGLVYELNKTDHISFLCGMNYTSKTLKLISGDAVICSGK 613

Query: 619 NIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNVASSGSTYRATISAPKG--LRITVNP 678
            + R  +LN PS +  ++  + +    F RT+TN+ ++ STY++ I    G  L + V+P
Sbjct: 614 TLQR--NLNYPSMSAKLSESNSSFTVTFKRTVTNLGTANSTYKSKIVLNHGSKLNVKVSP 673

Query: 679 SVLSFNRIGERKSFNLTVGGT-VSRRL-VSACLVWSDGVHSVRSPITI 694
           SVLS   + E++SF +TV G+ +  +L  SA L+WSDG H+VRSPI +
Sbjct: 674 SVLSMKSLKEKQSFTVTVSGSNIDPKLPSSANLIWSDGTHNVRSPIVV 705

BLAST of Csor.00g027350 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 7.0e-154
Identity = 316/746 (42.36%), Postives = 448/746 (60.05%), Query Frame = 0

Query: 9   SSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQ----DPSSAHLHHRAMLEQ 68
           +S  +L+ L +    SV A T     DD+++YIVYMGS        P+S H++   +L++
Sbjct: 6   ASSSLLSCLLVLFLSSVSAVT-----DDKQVYIVYMGSLSSRADYTPTSDHMN---ILQE 65

Query: 69  VVGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFL 128
           V G +     ++ +Y RSFNGF  RLTE E + +A   GVVSVF N+   L TT SWDF+
Sbjct: 66  VTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFM 125

Query: 129 GFSQNV--SRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSC 188
           G  + +   R   VES+ ++GV+DSGI PES SF+D+ FGP P  WKG+C  +   NF+C
Sbjct: 126 GLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVC--SGGKNFTC 185

Query: 189 NRKIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGA 248
           N K+IGAR Y              RD +GHGTHTAS  AG  V  AS + +G GT RGG 
Sbjct: 186 NNKLIGARDY---------TSEGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGV 245

Query: 249 PSARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHA 308
           P++R+A YK+C   GCS   +L+AFDDAIADGVD I++S+G      + +DPIAIG+FHA
Sbjct: 246 PASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHA 305

Query: 309 MKKGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRF--------- 368
           M KG+LT NSAGN GPK  + + ++PW+L+VAA+T +R FVT+V LG+G+          
Sbjct: 306 MAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAY 365

Query: 369 ------FQFLFTKFNA---------------IVPPS----------------LFSSLGAI 428
                 +  ++ K  A                V  S                +  S+GA+
Sbjct: 366 EMKGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAV 425

Query: 429 NGVIMQSGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIA 488
            G+I ++   D A  +PLPAA +  +D  ++  +   T +P A+  K+ A  + ++PVIA
Sbjct: 426 -GLIYRTPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIA 485

Query: 489 SFSSRGPNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHI 548
           SFSSRGPN++   ILKPD++ PGVEIL A+ P    ++  D+R   Y+++SGTSMSC H+
Sbjct: 486 SFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQ-DDTRHVKYSVLSGTSMSCPHV 545

Query: 549 TGVAVYVKTFNPTLSPAAIKSALMTTASPMNASLH--SQAEFAYGAGHVNPLKAVNPGLV 608
            GVA YVKTFNP  SP+ I+SA+MTTA P+NA+    +  EFAYG+GHV+P+ A NPGLV
Sbjct: 546 AGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLV 605

Query: 609 YDADENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALN 668
           Y+ D+++++ FLCG  Y + +++ I+G+   C+        +LN PS +  ++       
Sbjct: 606 YELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFT 665

Query: 669 QYFTRTLTNVASSGSTYRATISAPKG--LRITVNPSVLSFNRIGERKSFNLTVGGT--VS 697
             F RTLTNV +  STY + + A  G  L + + PSVLSF  + E++SF +TV G+   S
Sbjct: 666 VTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDS 725

BLAST of Csor.00g027350 vs. ExPASy Swiss-Prot
Match: F4KGD4 (Subtilisin-like protease SBT4.7 OS=Arabidopsis thaliana OX=3702 GN=SBT4.7 PE=3 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 2.7e-153
Identity = 311/711 (43.74%), Postives = 431/711 (60.62%), Query Frame = 0

Query: 18  FLALFCSVLASTSHSHDD--DRKIYIVYMGSKLQDPSSAHL-HHRAMLEQVVGSTFDPQS 77
           F+ LF   L+S S   DD  ++++Y+VYMGS         L HH ++L++V G +     
Sbjct: 9   FVVLF---LSSVSAVIDDPQNKQVYVVYMGSLPSLLEYTPLSHHMSILQEVTGDSSVEGR 68

Query: 78  VLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFLGFSQ--NVSR 137
           ++ +Y RSFNGF  RLTE E   +A  EGVVSVF N N  L TT SWDFLG  +  N  R
Sbjct: 69  LVRSYKRSFNGFAARLTESERIRVAEMEGVVSVFPNINYKLQTTASWDFLGLKEGKNTKR 128

Query: 138 VNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSCNRKIIGARAY 197
              +ES+ ++G +DSGIWPES SF+D+ FGP P  WKG+C  +   NF+CN K+IGAR Y
Sbjct: 129 NLAIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVC--SGGKNFTCNNKLIGARDY 188

Query: 198 HIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVYKI 257
                         RD  GHGTHTAS  AG  V+ AS + +G GTARGG P++RIA YK+
Sbjct: 189 ---------TSEGTRDLQGHGTHTASTAAGNAVADASFFGIGNGTARGGVPASRIAAYKV 248

Query: 258 CWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTSNS 317
           C +  C+ A +L+AFDDAIADGVD IS+S+ S  P+ Y+ D IAIG+FHA  KGILT NS
Sbjct: 249 CSEKDCTAASLLSAFDDAIADGVDLISISLASEFPQKYYKDAIAIGAFHANVKGILTVNS 308

Query: 318 AGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGR--------FFQFLFTKFNAI 377
           AGN G   STT S++PW+LSVAA+  +R F T+V LG+G+         F     K+  +
Sbjct: 309 AGNSGSFPSTTASVAPWILSVAASNTNRGFFTKVVLGNGKTLVGRSVNSFDLKGKKYPLV 368

Query: 378 VPPS----------LFSSLGAINGVIMQSGTRDNATSYPL----PAALVSRQDVITIFRF 437
              +          L S     + V + S   D+   Y L    P +L+   D  ++  +
Sbjct: 369 YGDNFNESLVQGKILVSKFPTSSKVAVGSILIDDYQHYALLSSKPFSLLPPDDFDSLVSY 428

Query: 438 TRETLTPYAMFFKSNATQDPSAPVIASFSSRGPNSLTNHILKPDLSGPGVEILGAWPPV- 497
              T +P   F K+ A  + +AP +ASFSSRGPN +   +LKPD+S PGVEIL A+ P+ 
Sbjct: 429 INSTRSPQGTFLKTEAFFNQTAPTVASFSSRGPNFIAVDLLKPDISAPGVEILAAYSPLG 488

Query: 498 APIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVKTFNPTLSPAAIKSALMTTASPM--N 557
           +P  E  D R   Y+++SGTSMSC H+ GVA Y++TF+P  SP+ I+SA+MTTA PM  N
Sbjct: 489 SPSEEESDKRRVKYSVMSGTSMSCPHVAGVAAYIRTFHPKWSPSVIQSAIMTTAWPMKPN 548

Query: 558 ASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVKFLCGQGYDTAMVRRITGDNSACT 617
               +  EFAYGAGHV+ + A+NPGLVY+ D+ +++ FLCG  Y +  +  I G+   C+
Sbjct: 549 RPGFASTEFAYGAGHVDQIAAINPGLVYELDKADHIAFLCGLNYTSKTLHLIAGEAVTCS 608

Query: 618 SGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNVASSGSTYRATISAPKGLR-ITVN 677
              + R  +LN PS +  +   + +    F RT+TN+ +  STY++ I    G + + V+
Sbjct: 609 GNTLPR--NLNYPSMSAKIDGYNSSFTVTFKRTVTNLGTPNSTYKSKIVLNHGAKLVKVS 668

Query: 678 PSVLSFNRIGERKSFNLTVGGTVSRRL-VSACLVWSDGVHSVRSPITISTS 697
           PSVLSF R+ E++SF +T  G ++  L  SA L+WSDG H+VRS I + T+
Sbjct: 669 PSVLSFKRVNEKQSFTVTFSGNLNLNLPTSANLIWSDGTHNVRSVIVVYTT 703

BLAST of Csor.00g027350 vs. NCBI nr
Match: KAG6600968.1 (hypothetical protein SDJN03_06201, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1382 bits (3578), Expect = 0.0
Identity = 697/697 (100.00%), Postives = 697/697 (100.00%), Query Frame = 0

Query: 1   MIRKGSNMSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRA 60
           MIRKGSNMSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRA
Sbjct: 1   MIRKGSNMSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRA 60

Query: 61  MLEQVVGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRS 120
           MLEQVVGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRS
Sbjct: 61  MLEQVVGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRS 120

Query: 121 WDFLGFSQNVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNF 180
           WDFLGFSQNVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNF
Sbjct: 121 WDFLGFSQNVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNF 180

Query: 181 SCNRKIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARG 240
           SCNRKIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARG
Sbjct: 181 SCNRKIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARG 240

Query: 241 GAPSARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSF 300
           GAPSARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSF
Sbjct: 241 GAPSARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSF 300

Query: 301 HAMKKGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFLFTK 360
           HAMKKGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFLFTK
Sbjct: 301 HAMKKGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFLFTK 360

Query: 361 FNAIVPPSLFSSLGAINGVIMQSGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAM 420
           FNAIVPPSLFSSLGAINGVIMQSGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAM
Sbjct: 361 FNAIVPPSLFSSLGAINGVIMQSGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAM 420

Query: 421 FFKSNATQDPSAPVIASFSSRGPNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRS 480
           FFKSNATQDPSAPVIASFSSRGPNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRS
Sbjct: 421 FFKSNATQDPSAPVIASFSSRGPNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRS 480

Query: 481 SLYNIISGTSMSCSHITGVAVYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGA 540
           SLYNIISGTSMSCSHITGVAVYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGA
Sbjct: 481 SLYNIISGTSMSCSHITGVAVYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGA 540

Query: 541 GHVNPLKAVNPGLVYDADENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLP 600
           GHVNPLKAVNPGLVYDADENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLP
Sbjct: 541 GHVNPLKAVNPGLVYDADENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLP 600

Query: 601 SFALSVAAGSKALNQYFTRTLTNVASSGSTYRATISAPKGLRITVNPSVLSFNRIGERKS 660
           SFALSVAAGSKALNQYFTRTLTNVASSGSTYRATISAPKGLRITVNPSVLSFNRIGERKS
Sbjct: 601 SFALSVAAGSKALNQYFTRTLTNVASSGSTYRATISAPKGLRITVNPSVLSFNRIGERKS 660

Query: 661 FNLTVGGTVSRRLVSACLVWSDGVHSVRSPITISTSR 697
           FNLTVGGTVSRRLVSACLVWSDGVHSVRSPITISTSR
Sbjct: 661 FNLTVGGTVSRRLVSACLVWSDGVHSVRSPITISTSR 697

BLAST of Csor.00g027350 vs. NCBI nr
Match: XP_022957349.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1278 bits (3306), Expect = 0.0
Identity = 661/736 (89.81%), Postives = 669/736 (90.90%), Query Frame = 0

Query: 8   MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 67
           MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG
Sbjct: 1   MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60

Query: 68  STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFLGFS 127
           STFDPQSVLYTYNRSFNGFVV LTEEEAQEIASKEGVVSVFLN+NNHLHTTRSWDF+GF 
Sbjct: 61  STFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120

Query: 128 QNVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSCNRKII 187
           QNV RV Q+ESNIVVGV+DSGIWPESPSFND+ FG  P  W+G CQ +N   FSCNRKII
Sbjct: 121 QNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSN---FSCNRKII 180

Query: 188 GARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARI 247
           GARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARI
Sbjct: 181 GARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARI 240

Query: 248 AVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGI 307
           AVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGI
Sbjct: 241 AVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGI 300

Query: 308 LTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFL-FTKFN---- 367
           LTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQ +    FN    
Sbjct: 301 LTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVY 360

Query: 368 -----------------------------------------AIVPPSLFSSLGAINGVIM 427
                                                    AIVPPSLFSSLGAINGVIM
Sbjct: 361 QYPLVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIM 420

Query: 428 QSGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSR 487
           QSGTRDNA SYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSR
Sbjct: 421 QSGTRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSR 480

Query: 488 GPNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAV 547
           GPNSL NHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAV
Sbjct: 481 GPNSLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAV 540

Query: 548 YVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENE 607
           YVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENE
Sbjct: 541 YVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENE 600

Query: 608 YVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTL 667
           YVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTL
Sbjct: 601 YVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTL 660

Query: 668 TNVASSGSTYRATISAPKGLRITVNPSVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWS 697
           TNVASSGSTYRATISAPKGLRITVNP VLSFNRIGERKSFNLTVGGTVSRRLVSACLVWS
Sbjct: 661 TNVASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWS 720

BLAST of Csor.00g027350 vs. NCBI nr
Match: XP_022957348.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1095 bits (2833), Expect = 0.0
Identity = 559/738 (75.75%), Postives = 615/738 (83.33%), Query Frame = 0

Query: 1   MIRKGSNMSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRA 60
           MIRKGS+MSSPLILNLLFLALFCSVLASTSHSHDDDR+IYIVYMGSKLQDPSSAHLHHRA
Sbjct: 1   MIRKGSDMSSPLILNLLFLALFCSVLASTSHSHDDDREIYIVYMGSKLQDPSSAHLHHRA 60

Query: 61  MLEQVVGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRS 120
           MLEQVVGSTFDPQSVLYTYNRSFNGFVV LTEEEAQEIASKEGVVSVFLN+NNHLHTTRS
Sbjct: 61  MLEQVVGSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRS 120

Query: 121 WDFLGFSQNVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNF 180
           WDF+GF QNV RV Q+ESNIVVGV+DSGIWPESPSFND+ FG  P  W+G CQ +N   F
Sbjct: 121 WDFMGFPQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSN---F 180

Query: 181 SCNRKIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARG 240
           SCNRK+IG RAYHIG+ IAP +V SPRDT+GHGTHTAS VAGGLVSQASLY LGLGTARG
Sbjct: 181 SCNRKLIGGRAYHIGRPIAPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARG 240

Query: 241 GAPSARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSF 300
           G PSARIA YK+CW+ GC DADILAAFDDAIADGVD +SLSVGS+KPRPYF+DPIAIGSF
Sbjct: 241 GVPSARIAAYKVCWEDGCWDADILAAFDDAIADGVDIVSLSVGSNKPRPYFNDPIAIGSF 300

Query: 301 HAMKKGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFL-FT 360
           HA+K GILTSNSAGNEGPKD TTTSLSPWLLSVAA+ MDRKFVTRV+LG+G  +Q +   
Sbjct: 301 HAIKNGILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISIN 360

Query: 361 KFN--------------------------------------------AIVPPSLFSSLGA 420
            FN                                            +++PPS F S GA
Sbjct: 361 TFNMNLQHPLVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFFSFGA 420

Query: 421 INGVIMQSGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVI 480
           I G++MQ+ TRDNA SYP PAA++   D I ++R+ R T+ P A  FKS A QD SAPV+
Sbjct: 421 IVGILMQAYTRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVV 480

Query: 481 ASFSSRGPNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSH 540
            SFSSRGPN+LTN ILKPDLSGPGVEIL AWPPVAPIA VKDSRSSLYNIISGTSMSC H
Sbjct: 481 VSFSSRGPNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPH 540

Query: 541 ITGVAVYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVY 600
           +TGVAVYVKTFNPTLSPAAIKSALMTTASPM+A  HSQAEFAYGAGHVNPLKA+NPGLVY
Sbjct: 541 LTGVAVYVKTFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVY 600

Query: 601 DADENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQ 660
           DA+E++YV FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLN PSFA+S++ G++ +NQ
Sbjct: 601 DANESDYVSFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNYPSFAVSIS-GTRTVNQ 660

Query: 661 YFTRTLTNVASSGSTYRATISAPKGLRITVNPSVLSFNRIGERKSFNLTVGGTVSRRLVS 693
           YFTRTLTNVA  GSTYRA ISAP+GL ITVNP+ LSFN IG+RKSF LTV GTVS+ + S
Sbjct: 661 YFTRTLTNVARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVRGTVSQSISS 720

BLAST of Csor.00g027350 vs. NCBI nr
Match: KAG6600967.1 (hypothetical protein SDJN03_06200, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1089 bits (2816), Expect = 0.0
Identity = 554/731 (75.79%), Postives = 609/731 (83.31%), Query Frame = 0

Query: 8   MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 67
           M S L+LNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG
Sbjct: 1   MCSSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60

Query: 68  STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFLGFS 127
           STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLN+NNHLHTTRSWDF+GF 
Sbjct: 61  STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120

Query: 128 QNVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSCNRKII 187
           QNV RV Q+ESNIVVGV+DSGIWPESPSFND+ FG  P  W+G CQ++N   FSCNRK+I
Sbjct: 121 QNVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDSN---FSCNRKLI 180

Query: 188 GARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARI 247
           G RAYHIG+ I P +V SPRDT+GHGTHTAS VAGGLVSQASLY LGLGTARGG PSARI
Sbjct: 181 GGRAYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARI 240

Query: 248 AVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGI 307
           A YK+CW+ GCSDADILAAFDDAIADGVD +SLSVGSSKPRPYFSDPIAIGSFHA+K GI
Sbjct: 241 AAYKVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGI 300

Query: 308 LTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFL-FTKFN---- 367
           LTSNSAGNEGPKD TTTSLSPWLLSVAA+ MDRKFVTRV+LG+G  +Q +    FN    
Sbjct: 301 LTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQ 360

Query: 368 ----------------------------------------AIVPPSLFSSLGAINGVIMQ 427
                                                   +++PPS FSS GAI G++MQ
Sbjct: 361 HPLVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQ 420

Query: 428 SGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRG 487
           + TRDNA SYP PAA++   D I ++R+ R T+ P A  FKS A QD SAPV+ SFSSRG
Sbjct: 421 AYTRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRG 480

Query: 488 PNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVY 547
           PN+LTN ILKPDLSGPGVEIL AWPPVAPIA VKDSRSSLYNIISGTSMSC H+TGVAVY
Sbjct: 481 PNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVY 540

Query: 548 VKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEY 607
           VKTFNPTLSPAAIKSALMTTASPM+A  HSQAEFAYGAGHVNPLKA+NPGLVYDA+E++Y
Sbjct: 541 VKTFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDY 600

Query: 608 VKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLT 667
           V FLCGQGY T+MVRRITGDNSACTSGNIGRVWDLN PSFA+S++ G++ +NQYFTRTLT
Sbjct: 601 VSFLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSIS-GTRTVNQYFTRTLT 660

Query: 668 NVASSGSTYRATISAPKGLRITVNPSVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWSD 693
           NVA  GSTYRA ISAP+GL ITVNP+ LSFN IG+RKSF LTVGGTVS+ + SA L WSD
Sbjct: 661 NVARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSD 720

BLAST of Csor.00g027350 vs. NCBI nr
Match: XP_023527145.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1069 bits (2765), Expect = 0.0
Identity = 546/738 (73.98%), Postives = 606/738 (82.11%), Query Frame = 0

Query: 1   MIRKGSNMSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRA 60
           M RKG  M S L+ NLLFLALF SVLASTSHSH DDRKIYIVYMGSKL+DPSSAHLHHRA
Sbjct: 1   MTRKGITMCSSLLFNLLFLALFRSVLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRA 60

Query: 61  MLEQVVGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRS 120
           MLEQVVGSTFDPQSVLYTY RSFNGFVVRLTEEEAQEIASKEGVVSVFLN+NNHLHTTRS
Sbjct: 61  MLEQVVGSTFDPQSVLYTYTRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRS 120

Query: 121 WDFLGFSQNVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNF 180
           WDFLGF QNV RV Q+ESNIVVGV+DSGIWPESPSFND+ FG  P  W+G CQ +N   F
Sbjct: 121 WDFLGFPQNVGRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGSCQVSN---F 180

Query: 181 SCNRKIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARG 240
            CNRK+IG RAY IG+ I P +V SPRDT+GHGTHTAS VAGGLVSQASLY LG+GTARG
Sbjct: 181 RCNRKLIGGRAYRIGRPILPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGIGTARG 240

Query: 241 GAPSARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSF 300
           G PSARIA YK+CWK GCSDADILAAFDDAIADGVD +SLSVGSS+PRPYF+DPIAIGSF
Sbjct: 241 GVPSARIAAYKVCWKDGCSDADILAAFDDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSF 300

Query: 301 HAMKKGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFLFTK 360
           HA+K GILTSNSAGNEGPKD TTTSLSPWLLSVAA+ MDRKFVTRV+LG+G  ++ +   
Sbjct: 301 HAIKNGILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYEGISIN 360

Query: 361 --------------------FN-------------------------AIVPPSLFSSLGA 420
                               FN                         +++P S+FSS GA
Sbjct: 361 TFDMNLQYPLVYAGDIPNLGFNGSTSRYCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGA 420

Query: 421 INGVIMQSGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVI 480
           I G++MQ+ TRDNA SYP PAA++   D I ++R+ R T+ P A  FKS A QD SAP++
Sbjct: 421 IAGILMQAYTRDNARSYPAPAAVLHPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLV 480

Query: 481 ASFSSRGPNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSH 540
            SFSSRGPN+LTN ILKPDLSGPGVEIL AWPPVAPIA VKDSRSSLYNIISGTSMSC H
Sbjct: 481 VSFSSRGPNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPH 540

Query: 541 ITGVAVYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVY 600
           +TGVAVYVKTFNPTLSPAAIKSAL+TTASPM+A  HSQAEFAYGAGHVNPLKA+NPGLVY
Sbjct: 541 LTGVAVYVKTFNPTLSPAAIKSALITTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVY 600

Query: 601 DADENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQ 660
           DA+E++YV FLCGQGY T+MVRRITGDNSACTSGNIGRVWDLN PSFA+S++ G++ +NQ
Sbjct: 601 DANESDYVSFLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSIS-GTRTVNQ 660

Query: 661 YFTRTLTNVASSGSTYRATISAPKGLRITVNPSVLSFNRIGERKSFNLTVGGTVSRRLVS 693
           YFTRTLTNVA  GSTYRA ISAP+GL ITVNP+ LSFN IG+RKSF LTVGGTVS+ + S
Sbjct: 661 YFTRTLTNVARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISS 720

BLAST of Csor.00g027350 vs. ExPASy TrEMBL
Match: A0A6J1H1P2 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458775 PE=3 SV=1)

HSP 1 Score: 1278 bits (3306), Expect = 0.0
Identity = 661/736 (89.81%), Postives = 669/736 (90.90%), Query Frame = 0

Query: 8   MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 67
           MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG
Sbjct: 1   MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60

Query: 68  STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFLGFS 127
           STFDPQSVLYTYNRSFNGFVV LTEEEAQEIASKEGVVSVFLN+NNHLHTTRSWDF+GF 
Sbjct: 61  STFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120

Query: 128 QNVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSCNRKII 187
           QNV RV Q+ESNIVVGV+DSGIWPESPSFND+ FG  P  W+G CQ +N   FSCNRKII
Sbjct: 121 QNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSN---FSCNRKII 180

Query: 188 GARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARI 247
           GARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARI
Sbjct: 181 GARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARI 240

Query: 248 AVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGI 307
           AVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGI
Sbjct: 241 AVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGI 300

Query: 308 LTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFL-FTKFN---- 367
           LTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQ +    FN    
Sbjct: 301 LTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVY 360

Query: 368 -----------------------------------------AIVPPSLFSSLGAINGVIM 427
                                                    AIVPPSLFSSLGAINGVIM
Sbjct: 361 QYPLVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIM 420

Query: 428 QSGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSR 487
           QSGTRDNA SYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSR
Sbjct: 421 QSGTRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSR 480

Query: 488 GPNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAV 547
           GPNSL NHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAV
Sbjct: 481 GPNSLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAV 540

Query: 548 YVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENE 607
           YVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENE
Sbjct: 541 YVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENE 600

Query: 608 YVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTL 667
           YVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTL
Sbjct: 601 YVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTL 660

Query: 668 TNVASSGSTYRATISAPKGLRITVNPSVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWS 697
           TNVASSGSTYRATISAPKGLRITVNP VLSFNRIGERKSFNLTVGGTVSRRLVSACLVWS
Sbjct: 661 TNVASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWS 720

BLAST of Csor.00g027350 vs. ExPASy TrEMBL
Match: A0A6J1GZY5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458774 PE=3 SV=1)

HSP 1 Score: 1095 bits (2833), Expect = 0.0
Identity = 559/738 (75.75%), Postives = 615/738 (83.33%), Query Frame = 0

Query: 1   MIRKGSNMSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRA 60
           MIRKGS+MSSPLILNLLFLALFCSVLASTSHSHDDDR+IYIVYMGSKLQDPSSAHLHHRA
Sbjct: 1   MIRKGSDMSSPLILNLLFLALFCSVLASTSHSHDDDREIYIVYMGSKLQDPSSAHLHHRA 60

Query: 61  MLEQVVGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRS 120
           MLEQVVGSTFDPQSVLYTYNRSFNGFVV LTEEEAQEIASKEGVVSVFLN+NNHLHTTRS
Sbjct: 61  MLEQVVGSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRS 120

Query: 121 WDFLGFSQNVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNF 180
           WDF+GF QNV RV Q+ESNIVVGV+DSGIWPESPSFND+ FG  P  W+G CQ +N   F
Sbjct: 121 WDFMGFPQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSN---F 180

Query: 181 SCNRKIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARG 240
           SCNRK+IG RAYHIG+ IAP +V SPRDT+GHGTHTAS VAGGLVSQASLY LGLGTARG
Sbjct: 181 SCNRKLIGGRAYHIGRPIAPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARG 240

Query: 241 GAPSARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSF 300
           G PSARIA YK+CW+ GC DADILAAFDDAIADGVD +SLSVGS+KPRPYF+DPIAIGSF
Sbjct: 241 GVPSARIAAYKVCWEDGCWDADILAAFDDAIADGVDIVSLSVGSNKPRPYFNDPIAIGSF 300

Query: 301 HAMKKGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFL-FT 360
           HA+K GILTSNSAGNEGPKD TTTSLSPWLLSVAA+ MDRKFVTRV+LG+G  +Q +   
Sbjct: 301 HAIKNGILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISIN 360

Query: 361 KFN--------------------------------------------AIVPPSLFSSLGA 420
            FN                                            +++PPS F S GA
Sbjct: 361 TFNMNLQHPLVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFFSFGA 420

Query: 421 INGVIMQSGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVI 480
           I G++MQ+ TRDNA SYP PAA++   D I ++R+ R T+ P A  FKS A QD SAPV+
Sbjct: 421 IVGILMQAYTRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVV 480

Query: 481 ASFSSRGPNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSH 540
            SFSSRGPN+LTN ILKPDLSGPGVEIL AWPPVAPIA VKDSRSSLYNIISGTSMSC H
Sbjct: 481 VSFSSRGPNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPH 540

Query: 541 ITGVAVYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVY 600
           +TGVAVYVKTFNPTLSPAAIKSALMTTASPM+A  HSQAEFAYGAGHVNPLKA+NPGLVY
Sbjct: 541 LTGVAVYVKTFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVY 600

Query: 601 DADENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQ 660
           DA+E++YV FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLN PSFA+S++ G++ +NQ
Sbjct: 601 DANESDYVSFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNYPSFAVSIS-GTRTVNQ 660

Query: 661 YFTRTLTNVASSGSTYRATISAPKGLRITVNPSVLSFNRIGERKSFNLTVGGTVSRRLVS 693
           YFTRTLTNVA  GSTYRA ISAP+GL ITVNP+ LSFN IG+RKSF LTV GTVS+ + S
Sbjct: 661 YFTRTLTNVARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVRGTVSQSISS 720

BLAST of Csor.00g027350 vs. ExPASy TrEMBL
Match: A0A4P2YW59 (Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2 SV=1)

HSP 1 Score: 959 bits (2480), Expect = 0.0
Identity = 495/729 (67.90%), Postives = 574/729 (78.74%), Query Frame = 0

Query: 10  SPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVGST 69
           S +I  L+ L+L  S+LAS   S DD RKIYIVYMG+KL+DP SAHLHHRAMLE+VVGST
Sbjct: 8   SSVIFKLVLLSLLSSLLASGFDSDDDGRKIYIVYMGNKLEDPDSAHLHHRAMLEEVVGST 67

Query: 70  FDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFLGFSQN 129
           F P+SV+YTY RSFNGF V+LTEEEAQ+IA+KEGVVSVFLN+ NH+HTT SWDFLGF Q 
Sbjct: 68  FAPESVIYTYKRSFNGFAVKLTEEEAQKIAAKEGVVSVFLNQQNHIHTTNSWDFLGFPQA 127

Query: 130 VSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSCNRKIIGA 189
           V+R+ QVESNIVVGV+DSGIWPESPSF DE FG AP +WKG CQ + N  FSCN KIIGA
Sbjct: 128 VARMRQVESNIVVGVLDSGIWPESPSFRDEGFGRAPSSWKGTCQASKN--FSCNNKIIGA 187

Query: 190 RAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAV 249
           RAY +G ++ P +V+SPRDT+GHGTHTAS VAGGLVSQASLY LGLGTARGG PSARIA 
Sbjct: 188 RAYRVGGTLPPGDVSSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAA 247

Query: 250 YKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILT 309
           YKICW  GCSDADILAAFDDAIADGV  ISLSVG S+ RPYF+DPIAIG+FHAMK GILT
Sbjct: 248 YKICWSDGCSDADILAAFDDAIADGVHIISLSVGGSQARPYFNDPIAIGAFHAMKHGILT 307

Query: 310 SNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFLFTK--------- 369
           SNSAGNEG K  TTTSLSPWLLSVAA+T DRKFVT V+LG+G+ +Q              
Sbjct: 308 SNSAGNEGSKFFTTTSLSPWLLSVAASTTDRKFVTHVQLGNGKIYQGTAINTFDMNNQYP 367

Query: 370 -----------FN-------------------------AIVPPSLFSSLGAINGVIMQSG 429
                      FN                         +I+P S+FSS GAI GV+MQ G
Sbjct: 368 LVYAGEVPNVGFNGSTSRFCLNNSVNPYLVKGKILLCDSILPSSVFSSFGAIAGVLMQ-G 427

Query: 430 TRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPN 489
             DNA S+ +PA ++  QD   ++R+   T TP A        +D SAPV+ SFSSRGPN
Sbjct: 428 YTDNARSHSVPATVLHPQDGANVYRYIYSTSTPTARILSGTTVRDASAPVVISFSSRGPN 487

Query: 490 SLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVK 549
           +LTN +LKPD++GPGVEIL AWPP+API+ V++SRS+L+NIISGTSMSC H++ VAVYVK
Sbjct: 488 ALTNDLLKPDITGPGVEILAAWPPIAPISGVRESRSTLFNIISGTSMSCPHLSAVAVYVK 547

Query: 550 TFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVK 609
           TFNP+ SPAAIKSALMTTASPM A L+S+AEFAYG+GHVNPLKAVNPGLVYDAD+++YVK
Sbjct: 548 TFNPSWSPAAIKSALMTTASPMTARLNSEAEFAYGSGHVNPLKAVNPGLVYDADQSDYVK 607

Query: 610 FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNV 669
           FLCGQGY+TAM+RRITGDNSACTS NIGRVWDLN PSFAL+V+ G ++LNQYFTRTLTNV
Sbjct: 608 FLCGQGYNTAMLRRITGDNSACTSANIGRVWDLNYPSFALAVS-GYQSLNQYFTRTLTNV 667

Query: 670 ASSGSTYRATISAPKGLRITVNPSVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWSDGV 693
           AS  STYRATIS P+GL ITVNP VLSF+  G+RKSF LTV G+VS+ +VS  LVWSDGV
Sbjct: 668 ASQASTYRATISVPQGLTITVNPPVLSFSGKGDRKSFTLTVRGSVSQPIVSGSLVWSDGV 727

BLAST of Csor.00g027350 vs. ExPASy TrEMBL
Match: K7NBW1 (Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 934 bits (2413), Expect = 0.0
Identity = 488/733 (66.58%), Postives = 565/733 (77.08%), Query Frame = 0

Query: 6   SNMSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQV 65
           S MSS L+  L+FL+LFCS+L S+S S+DD RKIYIVYMGSKL+D +SAHL+HRAMLE+V
Sbjct: 4   STMSS-LVFKLIFLSLFCSLLVSSSDSNDDGRKIYIVYMGSKLEDTASAHLYHRAMLEEV 63

Query: 66  VGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFLG 125
           VGSTF P+SV+YTY RSFNGF V+LTEEEA +IA+KEGVVSVF +E NHLHTTRSWDFLG
Sbjct: 64  VGSTFAPESVIYTYKRSFNGFAVKLTEEEALKIAAKEGVVSVFPSEKNHLHTTRSWDFLG 123

Query: 126 FSQNVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSCNRK 185
            SQNV RV QVESNIVVGV DSGIWPE+PSFND+ FGPAP NW+G CQ + N  F CNRK
Sbjct: 124 ISQNVPRVKQVESNIVVGVFDSGIWPENPSFNDDGFGPAPANWRGTCQASTN--FRCNRK 183

Query: 186 IIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSA 245
           IIGARAY    ++ P +V SPRDT+GHGTHTAS VAG LVSQASLY LG+GTARGG P A
Sbjct: 184 IIGARAYRS-STLPPGDVRSPRDTDGHGTHTASTVAGVLVSQASLYGLGVGTARGGVPPA 243

Query: 246 RIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKK 305
           RIAVYKICW  GCSDADILAAFDDAIADGVD ISLSVG   P+PY  + IAIGSFHAMK+
Sbjct: 244 RIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGKVPQPYLYNSIAIGSFHAMKR 303

Query: 306 GILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFLFTK----- 365
           GILTSNSAGN GPK  T TSLSPWL +VAA++ DRKFVT+V LG+G  +Q +        
Sbjct: 304 GILTSNSAGNNGPKSFTVTSLSPWLPTVAASSSDRKFVTQVLLGNGNTYQGVSINTFDMR 363

Query: 366 ---------------FNAIVP-------------------------PSLFSSLGAINGVI 425
                          FN+                            P++F+S G   GV+
Sbjct: 364 NQYPLIYAGNAPSIGFNSSTSRYCYEDSVDPNLVRGKILLCDSTFGPTVFASFGGAAGVL 423

Query: 426 MQSGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSS 485
           MQS TRD+A+SYPLPA+++       I R+   T  P A  FKS   +D SAPV+ SFSS
Sbjct: 424 MQSNTRDHASSYPLPASVLDPAGGNNIKRYMSSTRAPTATIFKSTVVRDTSAPVVVSFSS 483

Query: 486 RGPNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVA 545
           RGPN +T+ ILKPD + PGVEIL AWPPVAPI+ V+DSRS+LYNIISGTSMSC H+T +A
Sbjct: 484 RGPNYVTHDILKPDSTAPGVEILAAWPPVAPISGVRDSRSALYNIISGTSMSCPHVTAIA 543

Query: 546 VYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADEN 605
           V++KTF P+ SPAAIKSALMTTASPMNA  +S AEFAYG+GHVNPLKAV+PGLVYDA E+
Sbjct: 544 VHIKTFYPSWSPAAIKSALMTTASPMNARFNSDAEFAYGSGHVNPLKAVDPGLVYDASES 603

Query: 606 EYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRT 665
           +YVKFLCG+GY TAMVR  TGDNSACTSGNIGRVWDLN PSFALS++  S+  NQ F RT
Sbjct: 604 DYVKFLCGEGYTTAMVRSTTGDNSACTSGNIGRVWDLNYPSFALSISR-SQTANQSFRRT 663

Query: 666 LTNVASSGSTYRATISAPKGLRITVNPSVLSFNRIGERKSFNLTVGGTVSRRLVSACLVW 693
           LTNV S  STYRA+ISAP+GL I+VNPSVLSFN IG++KSF LTV GTVS+ +VSA LVW
Sbjct: 664 LTNVVSGASTYRASISAPQGLSISVNPSVLSFNGIGDQKSFTLTVRGTVSQAIVSASLVW 723

BLAST of Csor.00g027350 vs. ExPASy TrEMBL
Match: A0A1S3CF86 (LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1)

HSP 1 Score: 918 bits (2372), Expect = 0.0
Identity = 479/734 (65.26%), Postives = 553/734 (75.34%), Query Frame = 0

Query: 8   MSSPLILNLLFLALFCSV-LASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVV 67
           MSS LI  L F +LF S  LAS   S DD + IYIVYMG KL+DP SAHLHHRAMLEQVV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 68  GSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFLGF 127
           GSTF P+SVL+TY RSFNGF V+LTEEEA++IAS EGVVSVFLNE N LHTTRSWDFLGF
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 128 SQNVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSCNRKI 187
              V R +QVESNIVVGV+D+GIWPESPSF+DE F P PP WKG C+ +NN  F CNRKI
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNN--FRCNRKI 180

Query: 188 IGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSAR 247
           IGAR+YHIG+ I+P +VN PRDTNGHGTHTAS  AGGLVSQA+LY LGLGTARGG P AR
Sbjct: 181 IGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLAR 240

Query: 248 IAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKG 307
           IA YK+CW  GCSDADILAA+DDAIADGVD ISLSVG + PR YF D IAIGSFHA+++G
Sbjct: 241 IAAYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERG 300

Query: 308 ILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFL--------- 367
           ILTSNSAGN GP   TT SLSPWLLSVAA+TMDRKFVT+V++G+G+ FQ +         
Sbjct: 301 ILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQY 360

Query: 368 --------------------FTKFNAIVPPSL----------------FSSLGAINGVIM 427
                               F   N++ P  L                F SL    GV+M
Sbjct: 361 YPLVSGRDIPNPGFDKSTSRFCTDNSVXPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLM 420

Query: 428 QSGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSR 487
            S TRD A SYPLP++++   D++   R+     +P A  FKS    + SAPV+ SFSSR
Sbjct: 421 TSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 480

Query: 488 GPNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAV 547
           GPN  T  ++KPD+SGPGVEIL AWP VAP+  ++  R++L+NIISGTSMSC HITG+A 
Sbjct: 481 GPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIR--RNTLFNIISGTSMSCPHITGIAT 540

Query: 548 YVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENE 607
           YVKT+NPT SPAAIKSALMTTASPMNA  + QAEFAYG+GHVNPLKAV PGLVYDA+E++
Sbjct: 541 YVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESD 600

Query: 608 YVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTL 667
           YVKFLCGQGY+T  VRRITGD SACT GN GRVWDLN PSF LSV+  SK  NQYF RTL
Sbjct: 601 YVKFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSP-SKTFNQYFNRTL 660

Query: 668 TNVASSGSTYRATISAPKGLRITVNPSVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWS 695
           T+VA   STYRA ISAP+GL I+VNP+VLSFN +G+RKSF LTV G++   +VSA LVWS
Sbjct: 661 TSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWS 720

BLAST of Csor.00g027350 vs. TAIR 10
Match: AT5G58840.1 (Subtilase family protein )

HSP 1 Score: 556.6 bits (1433), Expect = 2.8e-158
Identity = 311/719 (43.25%), Postives = 444/719 (61.75%), Query Frame = 0

Query: 13  ILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSS-----AHLHHRAMLEQVVG 72
           +++ + ++   SV A T  S   D+++Y+VYMGS    PSS        HH ++L++V G
Sbjct: 10  LISCVLVSFVISVSAVTDDS--QDKQVYVVYMGSL---PSSRLEYTPMSHHMSILQEVTG 69

Query: 73  STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFLGFS 132
            +     ++ +Y RSFNGF  RLTE E + +A  EGVVSVF + N  L TT SWDFLG  
Sbjct: 70  ESSVEGRLVRSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTASWDFLGLK 129

Query: 133 Q--NVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSCNRK 192
           +  N  R   +ES+ ++G +DSGIWPES SF+D+ FGP P  WKG+C  +   NF+CN K
Sbjct: 130 EGKNTKRNLAIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVC--SAGKNFTCNNK 189

Query: 193 IIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSA 252
           +IGAR Y              RD  GHGTHTAS  AG  V   S Y +G GTARGG P++
Sbjct: 190 LIGARDY---------TNEGTRDIEGHGTHTASTAAGNAVKNTSFYGIGNGTARGGVPAS 249

Query: 253 RIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKK 312
           RIA YK C + GC+   +L+AFDDAIADGVD IS+S+G++  R Y +DPIAIG+FHAM K
Sbjct: 250 RIAAYKACSEMGCTTESVLSAFDDAIADGVDLISISLGANLVRTYETDPIAIGAFHAMVK 309

Query: 313 GILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFLFTKFNA-- 372
           GILT  SAGN GP   +  S++PW+L+VAA+  +R FVT+V LG+G+   F+    NA  
Sbjct: 310 GILTVQSAGNGGPNPGSVMSVAPWILTVAASNTNRGFVTKVVLGNGK--TFVGKSLNAFD 369

Query: 373 ----------------IVPPSLFSSLGAINGVIMQSGTRDNATSYP----LPAALVSRQD 432
                           ++   +  S   ++  I+ +   +N   Y     LP++ +S+ D
Sbjct: 370 LKGKNYPLYGGSTDGPLLRGKILVSEDKVSSEIVVANINENYHDYAYVSILPSSALSKDD 429

Query: 433 VITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPNSLTNHILKPDLSGPGVEIL 492
             ++  +   T +P+    KS A  + +AP +A FSSRGPN++   ILKPD++ PGVEIL
Sbjct: 430 FDSVISYVNSTKSPHGTVLKSEAIFNQAAPKVAGFSSRGPNTIAVDILKPDVTAPGVEIL 489

Query: 493 GAWPPV-APIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVKTFNPTLSPAAIKSALMTT 552
            A+ P+ +P  + +D+R   Y+++SGTSMSC H+ GVA Y+KTF+P  SP+ I+SA+MTT
Sbjct: 490 AAFSPLNSPAQDKRDNRHVKYSVLSGTSMSCPHVAGVAAYIKTFHPEWSPSMIQSAIMTT 549

Query: 553 ASPMNA--SLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVKFLCGQGYDTAMVRRIT 612
           A PMNA  +  +  EFAYGAGHV+P+ A+NPGLVY+  +++++ FLCG  Y+   ++ I 
Sbjct: 550 AWPMNATGTAVASTEFAYGAGHVDPIAAINPGLVYEIGKSDHIAFLCGLNYNATSLKLIA 609

Query: 613 GDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNVASSGSTYRATISAPKG 672
           G+   CT   + R  +LN PS +  +     +    F RT+TNV +  STY++ I    G
Sbjct: 610 GEAVTCTGKTLPR--NLNYPSMSAKLPKSESSFIVTFNRTVTNVGTPNSTYKSKIVLNHG 669

Query: 673 --LRITVNPSVLSFNRIGERKSFNLTVGGT-VSRRL-VSACLVWSDGVHSVRSPITIST 696
             L++ V+PSVLS   + E++SF +TV G+ +  +L  SA L+WSDG H+VRSPI + T
Sbjct: 670 SNLKVEVSPSVLSMKSVKEKQSFTVTVSGSNIDPKLPSSANLIWSDGTHNVRSPIVVYT 708

BLAST of Csor.00g027350 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 545.8 bits (1405), Expect = 5.0e-155
Identity = 316/746 (42.36%), Postives = 448/746 (60.05%), Query Frame = 0

Query: 9   SSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQ----DPSSAHLHHRAMLEQ 68
           +S  +L+ L +    SV A T     DD+++YIVYMGS        P+S H++   +L++
Sbjct: 6   ASSSLLSCLLVLFLSSVSAVT-----DDKQVYIVYMGSLSSRADYTPTSDHMN---ILQE 65

Query: 69  VVGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFL 128
           V G +     ++ +Y RSFNGF  RLTE E + +A   GVVSVF N+   L TT SWDF+
Sbjct: 66  VTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFM 125

Query: 129 GFSQNV--SRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSC 188
           G  + +   R   VES+ ++GV+DSGI PES SF+D+ FGP P  WKG+C  +   NF+C
Sbjct: 126 GLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVC--SGGKNFTC 185

Query: 189 NRKIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGA 248
           N K+IGAR Y              RD +GHGTHTAS  AG  V  AS + +G GT RGG 
Sbjct: 186 NNKLIGARDY---------TSEGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGV 245

Query: 249 PSARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHA 308
           P++R+A YK+C   GCS   +L+AFDDAIADGVD I++S+G      + +DPIAIG+FHA
Sbjct: 246 PASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHA 305

Query: 309 MKKGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRF--------- 368
           M KG+LT NSAGN GPK  + + ++PW+L+VAA+T +R FVT+V LG+G+          
Sbjct: 306 MAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAY 365

Query: 369 ------FQFLFTKFNA---------------IVPPS----------------LFSSLGAI 428
                 +  ++ K  A                V  S                +  S+GA+
Sbjct: 366 EMKGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAV 425

Query: 429 NGVIMQSGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIA 488
            G+I ++   D A  +PLPAA +  +D  ++  +   T +P A+  K+ A  + ++PVIA
Sbjct: 426 -GLIYRTPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIA 485

Query: 489 SFSSRGPNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHI 548
           SFSSRGPN++   ILKPD++ PGVEIL A+ P    ++  D+R   Y+++SGTSMSC H+
Sbjct: 486 SFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQ-DDTRHVKYSVLSGTSMSCPHV 545

Query: 549 TGVAVYVKTFNPTLSPAAIKSALMTTASPMNASLH--SQAEFAYGAGHVNPLKAVNPGLV 608
            GVA YVKTFNP  SP+ I+SA+MTTA P+NA+    +  EFAYG+GHV+P+ A NPGLV
Sbjct: 546 AGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLV 605

Query: 609 YDADENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALN 668
           Y+ D+++++ FLCG  Y + +++ I+G+   C+        +LN PS +  ++       
Sbjct: 606 YELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFT 665

Query: 669 QYFTRTLTNVASSGSTYRATISAPKG--LRITVNPSVLSFNRIGERKSFNLTVGGT--VS 697
             F RTLTNV +  STY + + A  G  L + + PSVLSF  + E++SF +TV G+   S
Sbjct: 666 VTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDS 725

BLAST of Csor.00g027350 vs. TAIR 10
Match: AT5G58820.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 543.9 bits (1400), Expect = 1.9e-154
Identity = 311/711 (43.74%), Postives = 431/711 (60.62%), Query Frame = 0

Query: 18  FLALFCSVLASTSHSHDD--DRKIYIVYMGSKLQDPSSAHL-HHRAMLEQVVGSTFDPQS 77
           F+ LF   L+S S   DD  ++++Y+VYMGS         L HH ++L++V G +     
Sbjct: 9   FVVLF---LSSVSAVIDDPQNKQVYVVYMGSLPSLLEYTPLSHHMSILQEVTGDSSVEGR 68

Query: 78  VLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFLGFSQ--NVSR 137
           ++ +Y RSFNGF  RLTE E   +A  EGVVSVF N N  L TT SWDFLG  +  N  R
Sbjct: 69  LVRSYKRSFNGFAARLTESERIRVAEMEGVVSVFPNINYKLQTTASWDFLGLKEGKNTKR 128

Query: 138 VNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSCNRKIIGARAY 197
              +ES+ ++G +DSGIWPES SF+D+ FGP P  WKG+C  +   NF+CN K+IGAR Y
Sbjct: 129 NLAIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVC--SGGKNFTCNNKLIGARDY 188

Query: 198 HIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVYKI 257
                         RD  GHGTHTAS  AG  V+ AS + +G GTARGG P++RIA YK+
Sbjct: 189 ---------TSEGTRDLQGHGTHTASTAAGNAVADASFFGIGNGTARGGVPASRIAAYKV 248

Query: 258 CWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTSNS 317
           C +  C+ A +L+AFDDAIADGVD IS+S+ S  P+ Y+ D IAIG+FHA  KGILT NS
Sbjct: 249 CSEKDCTAASLLSAFDDAIADGVDLISISLASEFPQKYYKDAIAIGAFHANVKGILTVNS 308

Query: 318 AGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGR--------FFQFLFTKFNAI 377
           AGN G   STT S++PW+LSVAA+  +R F T+V LG+G+         F     K+  +
Sbjct: 309 AGNSGSFPSTTASVAPWILSVAASNTNRGFFTKVVLGNGKTLVGRSVNSFDLKGKKYPLV 368

Query: 378 VPPS----------LFSSLGAINGVIMQSGTRDNATSYPL----PAALVSRQDVITIFRF 437
              +          L S     + V + S   D+   Y L    P +L+   D  ++  +
Sbjct: 369 YGDNFNESLVQGKILVSKFPTSSKVAVGSILIDDYQHYALLSSKPFSLLPPDDFDSLVSY 428

Query: 438 TRETLTPYAMFFKSNATQDPSAPVIASFSSRGPNSLTNHILKPDLSGPGVEILGAWPPV- 497
              T +P   F K+ A  + +AP +ASFSSRGPN +   +LKPD+S PGVEIL A+ P+ 
Sbjct: 429 INSTRSPQGTFLKTEAFFNQTAPTVASFSSRGPNFIAVDLLKPDISAPGVEILAAYSPLG 488

Query: 498 APIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVKTFNPTLSPAAIKSALMTTASPM--N 557
           +P  E  D R   Y+++SGTSMSC H+ GVA Y++TF+P  SP+ I+SA+MTTA PM  N
Sbjct: 489 SPSEEESDKRRVKYSVMSGTSMSCPHVAGVAAYIRTFHPKWSPSVIQSAIMTTAWPMKPN 548

Query: 558 ASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVKFLCGQGYDTAMVRRITGDNSACT 617
               +  EFAYGAGHV+ + A+NPGLVY+ D+ +++ FLCG  Y +  +  I G+   C+
Sbjct: 549 RPGFASTEFAYGAGHVDQIAAINPGLVYELDKADHIAFLCGLNYTSKTLHLIAGEAVTCS 608

Query: 618 SGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNVASSGSTYRATISAPKGLR-ITVN 677
              + R  +LN PS +  +   + +    F RT+TN+ +  STY++ I    G + + V+
Sbjct: 609 GNTLPR--NLNYPSMSAKIDGYNSSFTVTFKRTVTNLGTPNSTYKSKIVLNHGAKLVKVS 668

Query: 678 PSVLSFNRIGERKSFNLTVGGTVSRRL-VSACLVWSDGVHSVRSPITISTS 697
           PSVLSF R+ E++SF +T  G ++  L  SA L+WSDG H+VRS I + T+
Sbjct: 669 PSVLSFKRVNEKQSFTVTFSGNLNLNLPTSANLIWSDGTHNVRSVIVVYTT 703

BLAST of Csor.00g027350 vs. TAIR 10
Match: AT5G58830.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 543.1 bits (1398), Expect = 3.2e-154
Identity = 301/708 (42.51%), Postives = 436/708 (61.58%), Query Frame = 0

Query: 19  LALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHL-HHRAMLEQVVGSTFDPQSVLY 78
           + LF S +++  +    D+++Y+VYMGS    P+   + +H  +L++V G          
Sbjct: 14  IILFLSSVSAIIYD-PQDKQVYVVYMGSLPSQPNYTPMSNHINILQEVTGE--------- 73

Query: 79  TYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFLGFSQ--NVSRVNQ 138
           +Y RSFNGF   LTE E + +A  EGVVSVF ++N  L TT SWDF+G  +  N  R   
Sbjct: 74  SYKRSFNGFSALLTESEREGVAEMEGVVSVFRSKNYKLQTTASWDFMGMKEGKNTKRNFA 133

Query: 139 VESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSCNRKIIGARAYHIG 198
           VES+ ++G +DSGIWPES SF+D+ FGP P  WKG+C+     NF+CN K+IGAR Y   
Sbjct: 134 VESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCK--GGKNFTCNNKLIGARDY--- 193

Query: 199 KSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVYKICWK 258
                      RD  GHGTHT S  AG  V+  S + +G GTARGG P++R+A YK+C  
Sbjct: 194 ------TSEGTRDLQGHGTHTTSTAAGNAVADTSFFGIGNGTARGGVPASRVAAYKVCTI 253

Query: 259 HGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTSNSAGN 318
            GCSD ++L+AFDDAIADGVD IS+S+G   P  Y  D IAIG+FHAM KGILT +SAGN
Sbjct: 254 TGCSDDNVLSAFDDAIADGVDLISVSLGGDYPSLYAEDTIAIGAFHAMAKGILTVHSAGN 313

Query: 319 EGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGR----------------------- 378
            GP  +T  S++PW+L+VAATT +R+F+T+V LG+G+                       
Sbjct: 314 AGPNPTTVVSVAPWMLTVAATTTNRRFLTKVVLGNGKTLVGKSVNAFDLKGKKYPLEYGD 373

Query: 379 FFQFLFTKFNAIVPPSLFSSLGAINGVIMQSGTRDNATSYPLPAALVSRQDVITIFRFTR 438
           +      K   +V   L  S  A++   + +  +D A+    P +++S+ D  ++  +  
Sbjct: 374 YLNESLVKGKILVSRYLSGSEVAVS--FITTDNKDYASISSRPLSVLSQDDFDSLVSYIN 433

Query: 439 ETLTPYAMFFKSNATQDPSAPVIASFSSRGPNSLTNHILKPDLSGPGVEILGAWPPVA-P 498
            T +P     K+ A  +  +P +ASFSSRGPN++   ILKPD+S PGVEIL A+ P++ P
Sbjct: 434 STRSPQGSVLKTEAIFNQLSPKVASFSSRGPNTIAVDILKPDISAPGVEILAAYSPLSLP 493

Query: 499 IAEVKDSRSSLYNIISGTSMSCSHITGVAVYVKTFNPTLSPAAIKSALMTTASPMNA--S 558
             + +D R   Y+++SGTSM+C H+TGVA Y+KTF+P  SP+ I+SA+MTTA  MNA  +
Sbjct: 494 SEDRRDKRRVKYSVLSGTSMACPHVTGVAAYIKTFHPDWSPSVIQSAIMTTAWQMNATGT 553

Query: 559 LHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVKFLCGQGYDTAMVRRITGDNSACTSG 618
                EFAYGAGHV+P+ A+NPGLVY+ ++ +++ FLCG  Y +  ++ I+GD   C+  
Sbjct: 554 GAESTEFAYGAGHVDPIAAINPGLVYELNKTDHISFLCGMNYTSKTLKLISGDAVICSGK 613

Query: 619 NIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNVASSGSTYRATISAPKG--LRITVNP 678
            + R  +LN PS +  ++  + +    F RT+TN+ ++ STY++ I    G  L + V+P
Sbjct: 614 TLQR--NLNYPSMSAKLSESNSSFTVTFKRTVTNLGTANSTYKSKIVLNHGSKLNVKVSP 673

Query: 679 SVLSFNRIGERKSFNLTVGGT-VSRRL-VSACLVWSDGVHSVRSPITI 694
           SVLS   + E++SF +TV G+ +  +L  SA L+WSDG H+VRSPI +
Sbjct: 674 SVLSMKSLKEKQSFTVTVSGSNIDPKLPSSANLIWSDGTHNVRSPIVV 696

BLAST of Csor.00g027350 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 542.0 bits (1395), Expect = 7.2e-154
Identity = 315/735 (42.86%), Postives = 437/735 (59.46%), Query Frame = 0

Query: 17  LFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQD----PSSAHLHHRAMLEQVVGSTFDP 76
           L+  L   +L+S S   D+D ++YIVYMGS        P+S H+   ++L+QV G +   
Sbjct: 10  LYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHM---SILQQVTGESSIE 69

Query: 77  QSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRSWDFLGFSQ--NV 136
             ++ +Y RSFNGF  RLTE E   IA  EGVVSVF N+   LHTT SWDF+G  +  N 
Sbjct: 70  GRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNT 129

Query: 137 SRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNFSCNRKIIGAR 196
            R   +ES+ ++GV+D+GIWPES SF+D+ FGP P  WKG+C  +   NF+CN K+IGAR
Sbjct: 130 KRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVC--SGGKNFTCNNKLIGAR 189

Query: 197 AYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVY 256
            Y              RDT+GHGTHTAS  AG  V   S + +G GT RGG P++RIA Y
Sbjct: 190 DY---------TSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAY 249

Query: 257 KICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTS 316
           K+C   GCS   +L++FDDAIADGVD I++S+G   P  +  DPIAIG+FHAM KGILT 
Sbjct: 250 KVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTV 309

Query: 317 NSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGR--------FFQFLFTKFN 376
           +SAGN GPK +T + ++PW+ +VAA+T +R F+T+V LG+G+         F     K+ 
Sbjct: 310 SSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYP 369

Query: 377 AIVPPSLFS--------------------------------------SLGAINGVIMQSG 436
            +   S  S                                      S+GAI  +I +S 
Sbjct: 370 LVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAI-AIIDKSP 429

Query: 437 TRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPN 496
             D A ++ LPA+ +  +D  ++  +     +P A   K+    + ++PVIASFSSRGPN
Sbjct: 430 RPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPN 489

Query: 497 SLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVK 556
           ++   ILKPD++ PGVEIL A+ P    +E  D+R   Y++ SGTSM+C H+ GVA YVK
Sbjct: 490 TIAVDILKPDITAPGVEILAAFSPNGEPSE-DDTRRVKYSVFSGTSMACPHVAGVAAYVK 549

Query: 557 TFNPTLSPAAIKSALMTTASPMNASLH--SQAEFAYGAGHVNPLKAVNPGLVYDADENEY 616
           TF P  SP+ I+SA+MTTA P+ A     +  EFAYGAGHV+P+ A+NPGLVY+ D+ ++
Sbjct: 550 TFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADH 609

Query: 617 VKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLT 676
           + FLCG  Y +  ++ I+GD   C+  N     +LN PS +  ++      +  F RTLT
Sbjct: 610 IAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLT 669

Query: 677 NVASSGSTYRATISAPKG--LRITVNPSVLSFNRIGERKSFNLTVGGT--VSRRLVSACL 694
           NV +  STY++ + A  G  L I V PSVL F  + E++SF++TV G+   S    SA L
Sbjct: 670 NVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANL 728

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q395471.3e-26464.85Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1[more]
Q9FIM54.0e-15743.25Subtilisin-like protease SBT4.9 OS=Arabidopsis thaliana OX=3702 GN=SBT4.9 PE=2 S... [more]
Q9FIM61.4e-15442.51Subtilisin-like protease SBT4.8 OS=Arabidopsis thaliana OX=3702 GN=SBT4.8 PE=3 S... [more]
Q9FIG27.0e-15442.36Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... [more]
F4KGD42.7e-15343.74Subtilisin-like protease SBT4.7 OS=Arabidopsis thaliana OX=3702 GN=SBT4.7 PE=3 S... [more]
Match NameE-valueIdentityDescription
KAG6600968.10.0100.00hypothetical protein SDJN03_06201, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022957349.10.089.81cucumisin-like [Cucurbita moschata][more]
XP_022957348.10.075.75cucumisin-like [Cucurbita moschata][more]
KAG6600967.10.075.79hypothetical protein SDJN03_06200, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023527145.10.073.98cucumisin-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1H1P20.089.81cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458775 PE=3 SV=1[more]
A0A6J1GZY50.075.75cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458774 PE=3 SV=1[more]
A0A4P2YW590.067.90Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2... [more]
K7NBW10.066.58Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1[more]
A0A1S3CF860.065.26LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G58840.12.8e-15843.25Subtilase family protein [more]
AT5G59120.15.0e-15542.36subtilase 4.13 [more]
AT5G58820.11.9e-15443.74Subtilisin-like serine endopeptidase family protein [more]
AT5G58830.13.2e-15442.51Subtilisin-like serine endopeptidase family protein [more]
AT5G59090.17.2e-15442.86subtilase 4.12 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 487..503
score: 41.38
coord: 208..221
score: 53.31
coord: 137..156
score: 30.91
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 40..116
e-value: 6.8E-14
score: 52.3
NoneNo IPR availableGENE3D3.50.30.30coord: 339..355
e-value: 5.7E-97
score: 327.4
coord: 371..428
e-value: 1.0E-63
score: 217.7
NoneNo IPR availableGENE3D2.60.40.2310coord: 566..696
e-value: 1.1E-39
score: 137.2
NoneNo IPR availablePANTHERPTHR10795:SF685SUBTILISIN-LIKE PROTEASE SBT4.3coord: 39..353
coord: 373..695
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 120..549
score: 27.871391
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 23..116
e-value: 4.2E-27
score: 96.4
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 138..541
e-value: 4.1E-46
score: 157.5
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 596..693
e-value: 3.6E-26
score: 91.3
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 429..562
e-value: 1.0E-63
score: 217.7
coord: 139..338
e-value: 5.7E-97
score: 327.4
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 131..550
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 39..353
coord: 373..695
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 114..524
e-value: 7.5779E-129
score: 381.946

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g027350.m01Csor.00g027350.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016020 membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity