Csor.00g027330 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g027330
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptioncucumisin-like
LocationCsor_Chr04: 5954939 .. 5958931 (-)
RNA-Seq ExpressionCsor.00g027330
SyntenyCsor.00g027330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACAATTGCATGGATTCTCTTTCGATGGCAGGCTTTTGCTAATGGCTTTTGCCGCCACTTTGCTTCTCACCTCCCAAGCAGCTGATGATAGAAAGGCATTTCATTCTCAAATTTTCATTTCCCAATTTCATTTTGATTTTATTTTAAGTAACATTATTTATATACATATAGTATGGGTAATTAATATCTACATTTTTAAAATGTAAGAGGTTTGATTAGAAAGATTATATTGTGGAGAGGTTAATGAAGTTTGCGTTTGAATGAGCATGCAGGTGTATATTGTGTACATGGGCAAACCATCTGAAGGAGGTTTCTCAGCTGTGTCTCAACGACACACCAACATGCTTCAACAAGTTCTTACCAATAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTGGTTCAATTAAAAATAATATCTCTAAACCTTTTCAAATATTTCAATAATAATCTTAAACTTTCAACAAATATCTTTGAAATACTCTTGAAAATGTTTCAAAAATACCCTTCAATTTTTTAAAAAAAGTTCAAAAATACCTTTATAGTAATTCCATGGTTAAAAAATAACATTGACACATGTTACTGTTATAAAAAAAAATAAAAAATAAAAACTTAACATCAATATATAAACGAAAATTGTCTATCGACACATCATAGTTTATTTAAGAACGTTTTAATGTTATTTTAGAAAGTTCTCGATAGTTTTTGAAATATGATATTTTTGAACTTTAAAAAAAAAAAATTAAGCATTTTTAAAGTTTTTTTTTTTTTTAATTTAAAGATACTTTTTGATTTTTTTTTTTAAATACTTATACTAACAAAACATTTCAAAACTAAAAAAAATATTTTTGAAACATTGTTTCCATCCAAAATTAAAACATTTTTGAATTTTTTTAGAAGTGGAAAGATATTTTTGAAATGTTTAGAAATATTATTAAAAATTTCTTAGATGTATTTTTGAGATAAAAGTATAAATTTTAAAAATATTTTTATTAATTGAGTACTTTTATTTTAATTTAACAATTGTAAGATCGTGAGAGCATAGTTTGTTATTGGTTGTTTTATACTTGGATTTAATACACATTTGGATTTATTTCTCTAACCATTCTTGAAGCTAAAGAATTTGCCACATTTTGTAATTAAATAGAAACAACTTTGAATCTTCTAGGGTTTAACTTTTCAAACTAACTATGATCTCAGTGATGCATCAAAATCTCTGGTGTATAGCTACCACCGGAGCTTCAACGGCTTTGCAGCCTGGCTCAACCAGGAGGAGGCTCGAAAATTCGCCAGTGGGTTCTTCTCTAAACACAAAAAGAAACAGTTTTATTTGTTTAGGGTTTTCTGAATTTTGATGGGATTTTGGTTTTTCAGAGATGGATGAAGTTGTTTCAGTGTTTCCGAGTGAAAAGAAACAGCTTCATACGACAAGATCATGGGATTTTATGGGTTTCTTCCAACAAGCTCGAAGATCGAGCTTGGAGAGCGATTTGGTCATCGGAATGTTGGATACTGGAATTTGGCCGGAATCTGAAAGCTTTTCCGATGAAGGGTTTGGTCCACCACCGCCAAAATGGAAAGGGGAATGCCAACCCGCTTCTAACTTCACCTGCAACAAGTAATCCTATTTTACACCTTCATTTTAGGGTTCTTTAATTTTAGCTATAGATTAAATAAGTAATTCCATTATAATTTATTATTTTTTTTTTTTTTGAATTTAAATAATTTTACATTTTTTCTAGGAAGAAAAATAGTATAATCTATTATTATTCAATGTTTTTAAATCCATAGATTAAACGAGTGTCAACTGTTTTTCCCTAAACCCTAAAGATGTGATTGAAAACTTTTTAAGTAAAATAATCGGAGCTCGATTCTTCCGGAGCGAACCTCTCGGCGAAGGCGATATTCTTTCTCCGAGGGATACAGAAGGCCATGGAACCCACACCTCATCTACTGCCGGCGGCAATTTGGTCTCCGGCGCCAGCCTCTTCGGCCTTGGTCTCGGCACCGCTCGGGGTGGCACTCCCTCCGCCCGCATAGCCGTCTACAAGATCTGCTGGTCCGATGGTTGCTTCGACGCCGATATCCTCGCGGCTTTCGACAATGCAATTGCCGATGGCGTGGACATCATCTCCCTTTCCGTCGGAGGCTTTATCGCCAAGGATTACTTCAACGATTCAATTGCGATTGGGGCTTTTCACGCCATGAAGAATGGGATTCTGACTTCGAATTCCGGTGGGAACTCTGGCCCTAGCCTTGGCAGCATCACGAATGTTTCTCCATGGTCGCTATCGGTGGCTGCTAGTACCATCGATAGGAAGTTTGTGACGAATGTGAAATTGGGTAATGGAGAATCTTCTCAGGTGAGCCCTGAATTGCTTTATCTTTTCATGAACGTGTTCTGTTCTGCAACGTTCGAGCCCTTCATTATTTTGCATGCCATCTATTTGTCAGGGGATCTCTGTGAACACCTTCCAACTTGGAGATAAGTTGATTCCACTTATATATGCTGGTGATGCTCCTAATACAACTGCCGGTTTCAATGAATCATTATCAAGGTAATTGAAGCTACAAGATTGGTAATTTTATGATAAATCTAAACGTACGCGCTCTGTTTTTGCCCAGTTGAAGAACAAGTGTTGGGTTTAGAGGAATTGAATTTGGATGTAAAAATGCAGGTTCTGCTTCCCAGGGTCTTTGGACGTGAACCAAGTTGAGGGAAAGATTGTTCTTTGCGATGAGATTAGCGATGGGGAAGCAGCTCTGAGTAGTGGTGCGGTTGGTACAATAATGCAAGATGGTGGTCTCCAAGATGCCGCCTTTCTTTTTCCCCTGCCCGCTTCCGTGTTAGACTTGAACGCTGGAAACAATGTTTTCCAGTACCTGAGATCAACCAGGTAATAATAATGGCACATACCTCAATGCTTACCGAATATTTGGACGTTTAGAATCCAAAACATTCATGTTTTTACTTTGATAGCAATCCGGAAGCCACCATAGAAAAGAGTACCACAATAGAGGATATGTCAGCTCCATCTGTTGTTTCCTTCTCATCAAGGGGTCCTAACCTAATTACACTAGACATTCTCAAGGTATTAATTCATCTTACTTACTTTCCTAATCACTTGATGAAGCCAAGTCCTCACTGAATTTTATCTTGTAGCCTGATTTGGCAGCACCAGGAGTGGATATAGTAGCATCTTGGTCTGAAGGTACAACAATTGGAGGCTTAGAAGGGGATAACCGAGTATCTCCATTTAACATAATCTCTGGCACATCCATGGCTTGCCCACATGCGACAGGAGCCGCTGCCTATGTCAAATCATTCCACCCAACTTGGTCTCCAGCTGCTATTAAGTCTGCACTTATGACAACAGGTAATAGTTTGAGCTTAATTGTTTAGTACCACTAGTTTAGCCTAACCTTAATCCTTAATCTGTTACAGCTTTTCCCATGGCTCCAAAGTTAAACACGGATGCTGAGTTTGCATATGGAGCAGGTCACATAAACCCAGTGAATGCCATAAACCCCGGTTTAGTGTATGATGCAGAGGAGATTGATTACATAAAGTTCTTATGTGGGCAAGGATATAGTACTCAAAATCTTCGTCTTGTTACCGGAGACGAAAGCAACTGTTCAAATGCTGCAAAAACAGCTGCATCAGATCTAAATTATCCGTCTTTTGGTCTATTAATGAAGTCCCAAAAATTGAATACTCGTGTCTACCACAGGACTGTCACAAACGTGGGGGAGCCAGTATCGACATATAAAGCAGTTATTCGAGCTCCGCCTGGGCTGGAAGTTACAGTACGTCCTGCTACTCTTTCTTTCCGTTCTCTTGGACAAAAGATGTCGTTCAGTGTGAGGGTTAAAGCAAATACTGGTGGGAATATTCTTTCTGGTAGCTTAAGCTGGGAGGATGGAGTGCATCTGGTGAGGAGCCCCATTGTTGCATTTGCCACTCCATCATGA

mRNA sequence

ATGGAACAATTGCATGGATTCTCTTTCGATGGCAGGCTTTTGCTAATGGCTTTTGCCGCCACTTTGCTTCTCACCTCCCAAGCAGCTGATGATAGAAAGGCATTTCATTCTCAAATTTTCATTTCCCAATTTCATTTTGATTTTATTTTAAGTAACATTATTTATATACATATAGTATGGGTGTATATTGTGTACATGGGCAAACCATCTGAAGGAGGTTTCTCAGCTGTGTCTCAACGACACACCAACATGCTTCAACAAGTTCTTACCAATAGTGATGCATCAAAATCTCTGGTGTATAGCTACCACCGGAGCTTCAACGGCTTTGCAGCCTGGCTCAACCAGGAGGAGGCTCGAAAATTCGCCAAGATGGATGAAGTTGTTTCAGTGTTTCCGAGTGAAAAGAAACAGCTTCATACGACAAGATCATGGGATTTTATGGGTTTCTTCCAACAAGCTCGAAGATCGAGCTTGGAGAGCGATTTGGTCATCGGAATGTTGGATACTGGAATTTGGCCGGAATCTGAAAGCTTTTCCGATGAAGGGTTTGGTCCACCACCGCCAAAATGGAAAGGGGAATGCCAACCCGCTTCTAACTTCACCTGCAACAATAAAATAATCGGAGCTCGATTCTTCCGGAGCGAACCTCTCGGCGAAGGCGATATTCTTTCTCCGAGGGATACAGAAGGCCATGGAACCCACACCTCATCTACTGCCGGCGGCAATTTGGTCTCCGGCGCCAGCCTCTTCGGCCTTGGTCTCGGCACCGCTCGGGGTGGCACTCCCTCCGCCCGCATAGCCGTCTACAAGATCTGCTGGTCCGATGGTTGCTTCGACGCCGATATCCTCGCGGCTTTCGACAATGCAATTGCCGATGGCGTGGACATCATCTCCCTTTCCGTCGGAGGCTTTATCGCCAAGGATTACTTCAACGATTCAATTGCGATTGGGGCTTTTCACGCCATGAAGAATGGGATTCTGACTTCGAATTCCGGTGGGAACTCTGGCCCTAGCCTTGGCAGCATCACGAATGTTTCTCCATGGTCGCTATCGGTGGCTGCTAGTACCATCGATAGGAAGTTTGTGACGAATGTGAAATTGGGTAATGGAGAATCTTCTCAGGGGATCTCTGTGAACACCTTCCAACTTGGAGATAAGTTGATTCCACTTATATATGCTGGTGATGCTCCTAATACAACTGCCGGTTTCAATGAATCATTATCAAGGTTCTGCTTCCCAGGGTCTTTGGACGTGAACCAAGTTGAGGGAAAGATTGTTCTTTGCGATGAGATTAGCGATGGGGAAGCAGCTCTGAGTAGTGGTGCGGTTGGTACAATAATGCAAGATGGTGGTCTCCAAGATGCCGCCTTTCTTTTTCCCCTGCCCGCTTCCGTGTTAGACTTGAACGCTGGAAACAATGTTTTCCAGTACCTGAGATCAACCAGCAATCCGGAAGCCACCATAGAAAAGAGTACCACAATAGAGGATATGTCAGCTCCATCTGTTGTTTCCTTCTCATCAAGGGGTCCTAACCTAATTACACTAGACATTCTCAAGCCTGATTTGGCAGCACCAGGAGTGGATATAGTAGCATCTTGGTCTGAAGGTACAACAATTGGAGGCTTAGAAGGGGATAACCGAGTATCTCCATTTAACATAATCTCTGGCACATCCATGGCTTGCCCACATGCGACAGGAGCCGCTGCCTATGTCAAATCATTCCACCCAACTTGGTCTCCAGCTGCTATTAAGTCTGCACTTATGACAACAGCTTTTCCCATGGCTCCAAAGTTAAACACGGATGCTGAGTTTGCATATGGAGCAGGTCACATAAACCCAGTGAATGCCATAAACCCCGGTTTAGTGTATGATGCAGAGGAGATTGATTACATAAAGTTCTTATGTGGGCAAGGATATAGTACTCAAAATCTTCGTCTTGTTACCGGAGACGAAAGCAACTGTTCAAATGCTGCAAAAACAGCTGCATCAGATCTAAATTATCCGTCTTTTGGTCTATTAATGAAGTCCCAAAAATTGAATACTCGTGTCTACCACAGGACTGTCACAAACGTGGGGGAGCCAGTATCGACATATAAAGCAGTTATTCGAGCTCCGCCTGGGCTGGAAGTTACAGTACGTCCTGCTACTCTTTCTTTCCGTTCTCTTGGACAAAAGATGTCGTTCAGTGTGAGGGTTAAAGCAAATACTGGTGGGAATATTCTTTCTGGTAGCTTAAGCTGGGAGGATGGAGTGCATCTGGTGAGGAGCCCCATTGTTGCATTTGCCACTCCATCATGA

Coding sequence (CDS)

ATGGAACAATTGCATGGATTCTCTTTCGATGGCAGGCTTTTGCTAATGGCTTTTGCCGCCACTTTGCTTCTCACCTCCCAAGCAGCTGATGATAGAAAGGCATTTCATTCTCAAATTTTCATTTCCCAATTTCATTTTGATTTTATTTTAAGTAACATTATTTATATACATATAGTATGGGTGTATATTGTGTACATGGGCAAACCATCTGAAGGAGGTTTCTCAGCTGTGTCTCAACGACACACCAACATGCTTCAACAAGTTCTTACCAATAGTGATGCATCAAAATCTCTGGTGTATAGCTACCACCGGAGCTTCAACGGCTTTGCAGCCTGGCTCAACCAGGAGGAGGCTCGAAAATTCGCCAAGATGGATGAAGTTGTTTCAGTGTTTCCGAGTGAAAAGAAACAGCTTCATACGACAAGATCATGGGATTTTATGGGTTTCTTCCAACAAGCTCGAAGATCGAGCTTGGAGAGCGATTTGGTCATCGGAATGTTGGATACTGGAATTTGGCCGGAATCTGAAAGCTTTTCCGATGAAGGGTTTGGTCCACCACCGCCAAAATGGAAAGGGGAATGCCAACCCGCTTCTAACTTCACCTGCAACAATAAAATAATCGGAGCTCGATTCTTCCGGAGCGAACCTCTCGGCGAAGGCGATATTCTTTCTCCGAGGGATACAGAAGGCCATGGAACCCACACCTCATCTACTGCCGGCGGCAATTTGGTCTCCGGCGCCAGCCTCTTCGGCCTTGGTCTCGGCACCGCTCGGGGTGGCACTCCCTCCGCCCGCATAGCCGTCTACAAGATCTGCTGGTCCGATGGTTGCTTCGACGCCGATATCCTCGCGGCTTTCGACAATGCAATTGCCGATGGCGTGGACATCATCTCCCTTTCCGTCGGAGGCTTTATCGCCAAGGATTACTTCAACGATTCAATTGCGATTGGGGCTTTTCACGCCATGAAGAATGGGATTCTGACTTCGAATTCCGGTGGGAACTCTGGCCCTAGCCTTGGCAGCATCACGAATGTTTCTCCATGGTCGCTATCGGTGGCTGCTAGTACCATCGATAGGAAGTTTGTGACGAATGTGAAATTGGGTAATGGAGAATCTTCTCAGGGGATCTCTGTGAACACCTTCCAACTTGGAGATAAGTTGATTCCACTTATATATGCTGGTGATGCTCCTAATACAACTGCCGGTTTCAATGAATCATTATCAAGGTTCTGCTTCCCAGGGTCTTTGGACGTGAACCAAGTTGAGGGAAAGATTGTTCTTTGCGATGAGATTAGCGATGGGGAAGCAGCTCTGAGTAGTGGTGCGGTTGGTACAATAATGCAAGATGGTGGTCTCCAAGATGCCGCCTTTCTTTTTCCCCTGCCCGCTTCCGTGTTAGACTTGAACGCTGGAAACAATGTTTTCCAGTACCTGAGATCAACCAGCAATCCGGAAGCCACCATAGAAAAGAGTACCACAATAGAGGATATGTCAGCTCCATCTGTTGTTTCCTTCTCATCAAGGGGTCCTAACCTAATTACACTAGACATTCTCAAGCCTGATTTGGCAGCACCAGGAGTGGATATAGTAGCATCTTGGTCTGAAGGTACAACAATTGGAGGCTTAGAAGGGGATAACCGAGTATCTCCATTTAACATAATCTCTGGCACATCCATGGCTTGCCCACATGCGACAGGAGCCGCTGCCTATGTCAAATCATTCCACCCAACTTGGTCTCCAGCTGCTATTAAGTCTGCACTTATGACAACAGCTTTTCCCATGGCTCCAAAGTTAAACACGGATGCTGAGTTTGCATATGGAGCAGGTCACATAAACCCAGTGAATGCCATAAACCCCGGTTTAGTGTATGATGCAGAGGAGATTGATTACATAAAGTTCTTATGTGGGCAAGGATATAGTACTCAAAATCTTCGTCTTGTTACCGGAGACGAAAGCAACTGTTCAAATGCTGCAAAAACAGCTGCATCAGATCTAAATTATCCGTCTTTTGGTCTATTAATGAAGTCCCAAAAATTGAATACTCGTGTCTACCACAGGACTGTCACAAACGTGGGGGAGCCAGTATCGACATATAAAGCAGTTATTCGAGCTCCGCCTGGGCTGGAAGTTACAGTACGTCCTGCTACTCTTTCTTTCCGTTCTCTTGGACAAAAGATGTCGTTCAGTGTGAGGGTTAAAGCAAATACTGGTGGGAATATTCTTTCTGGTAGCTTAAGCTGGGAGGATGGAGTGCATCTGGTGAGGAGCCCCATTGTTGCATTTGCCACTCCATCATGA

Protein sequence

MEQLHGFSFDGRLLLMAFAATLLLTSQAADDRKAFHSQIFISQFHFDFILSNIIYIHIVWVYIVYMGKPSEGGFSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEARKFAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGFGPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSSTAGGNLVSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEGKIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSNAAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTVRPATLSFRSLGQKMSFSVRVKANTGGNILSGSLSWEDGVHLVRSPIVAFATPS
Homology
BLAST of Csor.00g027330 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 9.5e-221
Identity = 396/702 (56.41%), Postives = 507/702 (72.22%), Query Frame = 0

Query: 61  VYIVYMGKPSEGGFSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEARK 120
           +YIVYMG+  E   SA    H  ML+QV+ ++ A +S++++Y RSFNGFA  L +EEA K
Sbjct: 33  IYIVYMGRKLEDPDSA-HLHHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEK 92

Query: 121 FAKMDEVVSVFPSEKKQLHTTRSWDFMGF-FQQARRSSLESDLVIGMLDTGIWPESESFS 180
            A M+ VVSVF +E  +LHTTRSWDF+GF     RRS +ES++V+G+LDTGIWPES SF 
Sbjct: 93  IASMEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFD 152

Query: 181 DEGFGPPPPKWKGECQPASNFTCNNKIIGARFFR-SEPLGEGDILSPRDTEGHGTHTSST 240
           DEGF PPPPKWKG C+ ++NF CN KIIGAR +    P+  GD+  PRDT GHGTHT+ST
Sbjct: 153 DEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTAST 212

Query: 241 AGGNLVSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIIS 300
           A G LVS A+L+GLGLGTARGG P ARIA YK+CW+DGC D DILAA+D+AIADGVDIIS
Sbjct: 213 AAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDTDILAAYDDAIADGVDIIS 272

Query: 301 LSVGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTID 360
           LSVGG   + YF D+IAIG+FHA++ GILTSNS GN GP+  +  ++SPW LSVAAST+D
Sbjct: 273 LSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMD 332

Query: 361 RKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDV 420
           RKFVT V++GNG+S QG+S+NTF   ++  PL+   D PNT  GF++S SRFC   S++ 
Sbjct: 333 RKFVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGRDIPNT--GFDKSTSRFCTDKSVNP 392

Query: 421 NQVEGKIVLCDEISDGEAALSS--GAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQ 480
           N ++GKIV+C+          S  GA G +M     +D A  +PLP+SVLD N      +
Sbjct: 393 NLLKGKIVVCEASFGPHEFFKSLDGAAGVLM-TSNTRDYADSYPLPSSVLDPNDLLATLR 452

Query: 481 YLRSTSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEG 540
           Y+ S  +P ATI KSTTI + SAP VVSFSSRGPN  T D++KPD++ PGV+I+A+W   
Sbjct: 453 YIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSV 512

Query: 541 TTIGGLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAP 600
             +GG+    R + FNIISGTSM+CPH TG A YVK+++PTWSPAAIKSALMTTA PM  
Sbjct: 513 APVGGI---RRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNA 572

Query: 601 KLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSN 660
           + N  AEFAYG+GH+NP+ A+ PGLVYDA E DY+KFLCGQGY+TQ +R +TGD S C++
Sbjct: 573 RFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTS 632

Query: 661 AAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTVRPAT 720
                  DLNYPSFGL +   +   + ++RT+T+V    STY+A+I AP GL ++V P  
Sbjct: 633 GNTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNV 692

Query: 721 LSFRSLGQKMSFSVRVKANTGGNILSGSLSWEDGVHLVRSPI 759
           LSF  LG + SF++ V+ +  G ++S SL W DGVH VRSPI
Sbjct: 693 LSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHYVRSPI 725

BLAST of Csor.00g027330 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 8.2e-188
Identity = 354/708 (50.00%), Postives = 466/708 (65.82%), Query Frame = 0

Query: 61  VYIVYMGKPSEGGFSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEARK 120
           VYIVYMG   E  +S  S  H ++LQ+++    AS  LV SY RSFNGFAA L+Q E++K
Sbjct: 32  VYIVYMGTLPEIKYSPPS-HHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQK 91

Query: 121 FAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSL-ESDLVIGMLDTGIWPESESFS 180
              M EVVSVFPS+  +L TTRSWDF+GF ++ARR S+ ESD+++G++D+GIWPESESF 
Sbjct: 92  LQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFD 151

Query: 181 DEGFGPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSSTA 240
           DEGFGPPP KWKG C+    F CNNK+IGARF+           S RD EGHGTHT+STA
Sbjct: 152 DEGFGPPPKKWKGSCKGGLKFACNNKLIGARFYNK------FADSARDEEGHGTHTASTA 211

Query: 241 GGNLVSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISL 300
            GN V  AS +GL  GTARGG PSARIA YK+C+ + C D DILAAFD+AIADGVD+IS+
Sbjct: 212 AGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISI 271

Query: 301 SVGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDR 360
           S+      +  N S+AIG+FHAM  GI+T+ S GN+GP  GS+ NVSPW ++VAAS  DR
Sbjct: 272 SISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDR 331

Query: 361 KFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVN 420
           +F+  V LGNG++  GISVNTF L     P++Y     N +   +++ + +C  G +D  
Sbjct: 332 QFIDRVVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDSE 391

Query: 421 QVEGKIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLR 480
            V+GKIVLCD+      A  +GA+G I+Q+  L D+AF+ P PAS L      ++  Y+ 
Sbjct: 392 LVKGKIVLCDDFLGYREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIE 451

Query: 481 STSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTI 540
           S   P+A I ++  I D  AP V SFSSRGP+ +  ++LKPD++APG++I+A++S   + 
Sbjct: 452 SAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASP 511

Query: 541 GGL--EGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPK 600
                  D R   ++++SGTSMACPH  G AAYVKSFHP WSP+AIKSA+MTTA PM  K
Sbjct: 512 SSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLK 571

Query: 601 LNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSNA 660
            N + EFAYG+G INP  A +PGLVY+ E  DY+K LC +G+ +  L   +G    CS  
Sbjct: 572 KNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSE- 631

Query: 661 AKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKA-VIRAPPGLEVTVRPAT 720
            +T   DLNYP+    + S       + RTVTNVG P STYKA V+   P L++++ P  
Sbjct: 632 -RTEVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEI 691

Query: 721 LSFRSLGQKMSFSVRV--KANTGGNILSGSLSWEDGVHLVRSPIVAFA 763
           L F  L +K SF V +  K    G+ +S S+ W DG H VRSPIVA++
Sbjct: 692 LRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 726

BLAST of Csor.00g027330 vs. ExPASy Swiss-Prot
Match: Q9LLL8 (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 2.2e-185
Identity = 352/716 (49.16%), Postives = 486/716 (67.88%), Query Frame = 0

Query: 62  YIVYMGKPSEGGFSAVSQRHTNMLQQV-LTNSDASKSLVYSYHRSFNGFAAWLNQEEARK 121
           YI+Y+G   +     + + H N+L  + ++  +A +  VYSY ++FN FAA L+  EA+K
Sbjct: 38  YIIYLGDRPDNTEETI-KTHINLLSSLNISQEEAKERKVYSYTKAFNAFAAKLSPHEAKK 97

Query: 122 FAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRS-SLESDLVIGMLDTGIWPESESFS 181
             +M+EVVSV  ++ ++LHTT+SWDF+G    A+R    E D++IG+LDTGI P+SESF 
Sbjct: 98  MMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRHLKAERDVIIGVLDTGITPDSESFL 157

Query: 182 DEGFGPPPPKWKGECQPASNFT-CNNKIIGARFFRSE-PLGEGDILSPRDTEGHGTHTSS 241
           D G GPPP KWKG C P  NFT CNNKIIGA++F+ +  +  G++ SP D +GHGTHTSS
Sbjct: 158 DHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKHDGNVPAGEVRSPIDIDGHGTHTSS 217

Query: 242 TAGGNLVSGASLFGLGLGTARGGTPSARIAVYKICWS-DGCFDADILAAFDNAIADGVDI 301
           T  G LV+ ASL+G+  GTARG  PSAR+A+YK+CW+  GC D DILA F+ AI DGV+I
Sbjct: 218 TVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCADMDILAGFEAAIHDGVEI 277

Query: 302 ISLSVGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAAST 361
           IS+S+GG IA DY +DSI++G+FHAM+ GILT  S GN GPS G++TN  PW L+VAAS 
Sbjct: 278 ISISIGGPIA-DYSSDSISVGSFHAMRKGILTVASAGNDGPSSGTVTNHEPWILTVAASG 337

Query: 362 IDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSL 421
           IDR F + + LGNG+S  G+ ++ F    K  PL+   DA   T   ++ L+R+CF  SL
Sbjct: 338 IDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVSGVDAAKNTD--DKYLARYCFSDSL 397

Query: 422 DVNQVEGKIVLCDEISDG-EAALSS-GAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNV 481
           D  +V+GK+++C     G E+ + S G  G I+      D A +F  PA+ ++ + G+ +
Sbjct: 398 DRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQYLDNAQIFMAPATSVNSSVGDII 457

Query: 482 FQYLRSTSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWS 541
           ++Y+ ST +  A I+K+  +  + AP V SFSSRGPN  ++ +LKPD+AAPG+DI+A+++
Sbjct: 458 YRYINSTRSASAVIQKTRQV-TIPAPFVASFSSRGPNPGSIRLLKPDIAAPGIDILAAFT 517

Query: 542 EGTTIGGLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPM 601
              ++ GL+GD + S F I+SGTSMACPH  G AAYVKSFHP W+PAAIKSA++T+A P+
Sbjct: 518 LKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKSFHPDWTPAAIKSAIITSAKPI 577

Query: 602 APKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDES-N 661
           + ++N DAEFAYG G INP  A +PGLVYD ++I Y++FLCG+GY+   L  + G  S +
Sbjct: 578 SRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDISYVQFLCGEGYNATTLAPLVGTRSVS 637

Query: 662 CSNAAKTAASD-LNYPSFGLLMKSQKLNT-RVYHRTVTNVGEPVSTYKAVIRAPPGLEVT 721
           CS+       D LNYP+  L ++S K +T  V+ R VTNVG P S Y A +RAP G+E+T
Sbjct: 638 CSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFRRRVTNVGPPSSVYTATVRAPKGVEIT 697

Query: 722 VRPATLSFRSLGQKMSFSVRVKAN--TGGNILSGSLSWEDGVHLVRSPIVAFATPS 766
           V P +LSF    QK SF V VKA   T G I+SG L W+   H VRSPIV ++  S
Sbjct: 698 VEPQSLSFSKASQKRSFKVVVKAKQMTPGKIVSGLLVWKSPRHSVRSPIVIYSPTS 748

BLAST of Csor.00g027330 vs. ExPASy Swiss-Prot
Match: F4JA91 (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 4.8e-180
Identity = 357/714 (50.00%), Postives = 468/714 (65.55%), Query Frame = 0

Query: 62  YIVYMGK-PSEGGFSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEARK 121
           YIVYMG  P+   +  +S  HT++LQ V   S     LV +Y RSFNGFAA L + E   
Sbjct: 35  YIVYMGALPARVDYMPMS-HHTSILQDVTGESSIEDRLVRNYKRSFNGFAARLTKSEREI 94

Query: 122 FAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQQ---ARRSSLESDLVIGMLDTGIWPESES 181
            A MDEVVSVFP++K +L TT SW+FMG  +     R + +ESD +IG++D+GI+PES+S
Sbjct: 95  LASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRTKRNTIIESDTIIGVIDSGIYPESDS 154

Query: 182 FSDEGFGPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSS 241
           FS +GFGPPP KWKG C+   NFT NNK+IGAR++   P  EG   S RD  GHG+HT+S
Sbjct: 155 FSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYY--TPKLEGFPESARDYMGHGSHTAS 214

Query: 242 TAGGNLVSGASLFGLGLGTARGGTPSARIAVYKIC--WSDGCFDADILAAFDNAIADGVD 301
           TA GN V   S +GLG GTARGG P+ARIAVYK+C    DGC    ILAAFD+AIAD VD
Sbjct: 215 TAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDPGVDGCTTDGILAAFDDAIADKVD 274

Query: 302 IISLSVGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAAS 361
           II++S+GG  +  +  D IAIGAFHAM  GIL  NS GNSGP   ++ +++PW  +VAAS
Sbjct: 275 IITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSAGNSGPEPSTVASIAPWMFTVAAS 334

Query: 362 TIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGS 421
             +R FVT V LGNG+ + G SVN+F L  K  PL+Y G + +++ G   + + FC PG 
Sbjct: 335 NTNRAFVTKVVLGNGK-TVGRSVNSFDLNGKKYPLVY-GKSASSSCG--AASAGFCSPGC 394

Query: 422 LDVNQVEGKIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVF 481
           LD  +V+GKIVLCD   + + A + GA+ +I++     D A +F  P SVL  +  N V 
Sbjct: 395 LDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVR-SHRTDVASIFSFPVSVLLEDDYNTVL 454

Query: 482 QYLRSTSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSE 541
            Y+ ST NP+A + KS TI +  AP V S+ SRGPN I  DILKPD+ APG +IVA++S 
Sbjct: 455 SYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIPDILKPDITAPGSEIVAAYSP 514

Query: 542 GTTIGGLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPM- 601
                    D R   +++ +GTSM+CPH  G AAY+KSFHP WSP+ I+SA+MTTA+PM 
Sbjct: 515 DAPPS--ISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFHPRWSPSMIQSAIMTTAWPMN 574

Query: 602 --APKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDES 661
                 N  AEFAYGAGH++P+ AI+PGLVY+A + D+I FLCG  Y+ +NLRL++GD S
Sbjct: 575 ASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIAFLCGLNYTAKNLRLISGDSS 634

Query: 662 NCS-NAAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVT 721
           +C+    K+   +LNYPS    + + K    ++ RTVTNVG P +TYKA +     L+V 
Sbjct: 635 SCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNVGRPNATYKAKV-VGSKLKVK 694

Query: 722 VRPATLSFRSLGQKMSFSVRVK--ANTGGNILSGSLSWEDGVHLVRSPIVAFAT 764
           V PA LS +SL +K SF+V          N++S  L W DGVH VRSPIV +AT
Sbjct: 695 VVPAVLSLKSLYEKKSFTVTASGAGPKAENLVSAQLIWSDGVHFVRSPIVVYAT 737

BLAST of Csor.00g027330 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 3.1e-179
Identity = 355/714 (49.72%), Postives = 464/714 (64.99%), Query Frame = 0

Query: 61  VYIVYMGK-PSEGGFSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEAR 120
           VYIVYMG  PS   +  +S  HT++LQ V   S     LV +Y RSFNGFAA L + E  
Sbjct: 33  VYIVYMGALPSRVDYMPMS-HHTSILQDVTGESSIQDRLVRNYKRSFNGFAARLTESERE 92

Query: 121 KFAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQ---QARRSSLESDLVIGMLDTGIWPESE 180
             A MDEVVSVFPS+   L TT SW+FMG  +     R   +ESD +IG++D+GI+PES+
Sbjct: 93  ILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKRNPLIESDTIIGVIDSGIYPESD 152

Query: 181 SFSDEGFGPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTS 240
           SFS +GFGPPP KWKG C+  +NFTCNNK+IGAR++   P  EG   S RD  GHG+HT+
Sbjct: 153 SFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYY--TPKLEGFPESARDNTGHGSHTA 212

Query: 241 STAGGNLVSGASLFGLGLGTARGGTPSARIAVYKICWSD--GCFDADILAAFDNAIADGV 300
           S A GN V   S +GLG GT RGG P+ARIAVYK+C      C    ILAAFD+AIAD V
Sbjct: 213 SIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGVIRCTSDGILAAFDDAIADKV 272

Query: 301 DIISLSVGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAA 360
           DII++S+G      +  D++AIGAFHAM  GILT N  GN+GP   +I +++PW  +VAA
Sbjct: 273 DIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGNNGPERRTIVSMAPWLFTVAA 332

Query: 361 STIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPG 420
           S ++R F+T V LGNG++  G SVN+F L  K  PL+Y   A   ++  + S + FC PG
Sbjct: 333 SNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVYGKSA---SSRCDASSAGFCSPG 392

Query: 421 SLDVNQVEGKIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNV 480
            LD  +V+GKIVLCD   +   A + GAV +I+++   +DAA +F  P SVL  +  N V
Sbjct: 393 CLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRN-PYEDAASVFSFPVSVLSEDDYNIV 452

Query: 481 FQYLRSTSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWS 540
             Y+ ST NP+A + KS TI +  AP V S+SSRGPN +  DILKPD+ APG +I+A++S
Sbjct: 453 LSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAYS 512

Query: 541 EGTTIGGLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPM 600
               +   E D R   + +ISGTSM+CPH  G AAY+K+FHP WSP+ I+SA+MTTA+PM
Sbjct: 513 --PYVPPSESDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPM 572

Query: 601 ---APKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDE 660
                  N  AEFAYGAGH++P+ AI+PGLVY+A + D+I FLCG  Y+ + LRL++GD 
Sbjct: 573 NASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDS 632

Query: 661 SNCS-NAAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEV 720
           S+C+    K+   +LNYPS    +   K     + RTVTNVG P +TYKA +     L+V
Sbjct: 633 SSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKV-VGSKLKV 692

Query: 721 TVRPATLSFRSLGQKMSFSVRVK--ANTGGNILSGSLSWEDGVHLVRSPIVAFA 763
            V PA LS +SL +K SF+V V        N++S  L W DGVH VRSPIV +A
Sbjct: 693 KVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVVYA 736

BLAST of Csor.00g027330 vs. NCBI nr
Match: KAG6600966.1 (hypothetical protein SDJN03_06199, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1517 bits (3928), Expect = 0.0
Identity = 765/765 (100.00%), Postives = 765/765 (100.00%), Query Frame = 0

Query: 1   MEQLHGFSFDGRLLLMAFAATLLLTSQAADDRKAFHSQIFISQFHFDFILSNIIYIHIVW 60
           MEQLHGFSFDGRLLLMAFAATLLLTSQAADDRKAFHSQIFISQFHFDFILSNIIYIHIVW
Sbjct: 1   MEQLHGFSFDGRLLLMAFAATLLLTSQAADDRKAFHSQIFISQFHFDFILSNIIYIHIVW 60

Query: 61  VYIVYMGKPSEGGFSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEARK 120
           VYIVYMGKPSEGGFSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEARK
Sbjct: 61  VYIVYMGKPSEGGFSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEARK 120

Query: 121 FAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSD 180
           FAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSD
Sbjct: 121 FAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSD 180

Query: 181 EGFGPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSSTAG 240
           EGFGPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSSTAG
Sbjct: 181 EGFGPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSSTAG 240

Query: 241 GNLVSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLS 300
           GNLVSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLS
Sbjct: 241 GNLVSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLS 300

Query: 301 VGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRK 360
           VGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRK
Sbjct: 301 VGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRK 360

Query: 361 FVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQ 420
           FVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQ
Sbjct: 361 FVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQ 420

Query: 421 VEGKIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRS 480
           VEGKIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRS
Sbjct: 421 VEGKIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRS 480

Query: 481 TSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIG 540
           TSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIG
Sbjct: 481 TSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIG 540

Query: 541 GLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPKLNT 600
           GLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPKLNT
Sbjct: 541 GLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPKLNT 600

Query: 601 DAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSNAAKT 660
           DAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSNAAKT
Sbjct: 601 DAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSNAAKT 660

Query: 661 AASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTVRPATLSFR 720
           AASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTVRPATLSFR
Sbjct: 661 AASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTVRPATLSFR 720

Query: 721 SLGQKMSFSVRVKANTGGNILSGSLSWEDGVHLVRSPIVAFATPS 765
           SLGQKMSFSVRVKANTGGNILSGSLSWEDGVHLVRSPIVAFATPS
Sbjct: 721 SLGQKMSFSVRVKANTGGNILSGSLSWEDGVHLVRSPIVAFATPS 765

BLAST of Csor.00g027330 vs. NCBI nr
Match: KAG7031587.1 (hypothetical protein SDJN02_05628, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1386 bits (3587), Expect = 0.0
Identity = 702/711 (98.73%), Postives = 702/711 (98.73%), Query Frame = 0

Query: 61  VYIVYMGKPSEGGFSAVSQRHTNMLQQVLTN------SDASKSLVYSYHRSFNGFAAWLN 120
           VYIVYMGKPSEGGFSAVSQRHTNMLQQ LT       SDASKSLVYSYHRSFNGFAAWLN
Sbjct: 3   VYIVYMGKPSEGGFSAVSQRHTNMLQQGLTFQTNYDLSDASKSLVYSYHRSFNGFAAWLN 62

Query: 121 QEEARKFAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPE 180
           QEEARKFAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPE
Sbjct: 63  QEEARKFAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPE 122

Query: 181 SESFSDEGFGPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTH 240
           SESFSDEGFGPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTH
Sbjct: 123 SESFSDEGFGPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTH 182

Query: 241 TSSTAGGNLVSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGV 300
           TSSTAGGNLVSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGV
Sbjct: 183 TSSTAGGNLVSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGV 242

Query: 301 DIISLSVGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAA 360
           DIISLSVGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAA
Sbjct: 243 DIISLSVGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAA 302

Query: 361 STIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPG 420
           STIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPG
Sbjct: 303 STIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPG 362

Query: 421 SLDVNQVEGKIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNV 480
           SLDVNQVEGKIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNV
Sbjct: 363 SLDVNQVEGKIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNV 422

Query: 481 FQYLRSTSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWS 540
           FQYLRSTSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWS
Sbjct: 423 FQYLRSTSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWS 482

Query: 541 EGTTIGGLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPM 600
           EGTTIGGLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPM
Sbjct: 483 EGTTIGGLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPM 542

Query: 601 APKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNC 660
           APKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNC
Sbjct: 543 APKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNC 602

Query: 661 SNAAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTVRP 720
           SNAAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTVRP
Sbjct: 603 SNAAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTVRP 662

Query: 721 ATLSFRSLGQKMSFSVRVKANTGGNILSGSLSWEDGVHLVRSPIVAFATPS 765
           ATLSFRSLGQKMSFSVRVKANTGGNILSGSLSWEDGVHLVRSPIVAFA PS
Sbjct: 663 ATLSFRSLGQKMSFSVRVKANTGGNILSGSLSWEDGVHLVRSPIVAFAIPS 713

BLAST of Csor.00g027330 vs. NCBI nr
Match: XP_022990086.1 (cucumisin-like [Cucurbita maxima])

HSP 1 Score: 1362 bits (3525), Expect = 0.0
Identity = 683/700 (97.57%), Postives = 691/700 (98.71%), Query Frame = 0

Query: 66  MGKPSEGGFSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEARKFAKMD 125
           MGKPSEGGFSAVSQRHT+MLQQVLTNSDASKSLVYSYHRSFNGFAAWLN+EEARKFAKMD
Sbjct: 1   MGKPSEGGFSAVSQRHTHMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNEEEARKFAKMD 60

Query: 126 EVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGFGP 185
           EVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEG GP
Sbjct: 61  EVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGLGP 120

Query: 186 PPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSSTAGGNLVS 245
           PPPKWKG CQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHT+STAGGNLVS
Sbjct: 121 PPPKWKGGCQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTASTAGGNLVS 180

Query: 246 GASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGGFI 305
           GASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGGFI
Sbjct: 181 GASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGGFI 240

Query: 306 AKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVTNV 365
           AKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVTNV
Sbjct: 241 AKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVTNV 300

Query: 366 KLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEGKI 425
           KLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEGKI
Sbjct: 301 KLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEGKI 360

Query: 426 VLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSNPE 485
           VLCDEI DGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSNPE
Sbjct: 361 VLCDEIGDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSNPE 420

Query: 486 ATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLEGD 545
           ATIEKSTTIED+SAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLEGD
Sbjct: 421 ATIEKSTTIEDLSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLEGD 480

Query: 546 NRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPKLNTDAEFA 605
           NRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPKLNTDAEFA
Sbjct: 481 NRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPKLNTDAEFA 540

Query: 606 YGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSNAAKTAASDL 665
           YGAGHINP NAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCS+ AKTAASDL
Sbjct: 541 YGAGHINPANAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSDVAKTAASDL 600

Query: 666 NYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTVRPATLSFRSLGQK 725
           NYPSF LLMKSQKLNTRVYHRTVTNVG PVSTYKAVIRAPPGLEVTVRPATLSFRSLGQK
Sbjct: 601 NYPSFSLLMKSQKLNTRVYHRTVTNVGVPVSTYKAVIRAPPGLEVTVRPATLSFRSLGQK 660

Query: 726 MSFSVRVKANTGGNILSGSLSWEDGVHLVRSPIVAFATPS 765
           MSF+VRV+AN+GGNILSGSLSWEDGVHLVRSPIVAF  PS
Sbjct: 661 MSFTVRVRANSGGNILSGSLSWEDGVHLVRSPIVAFGIPS 700

BLAST of Csor.00g027330 vs. NCBI nr
Match: XP_022956485.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1250 bits (3234), Expect = 0.0
Identity = 633/653 (96.94%), Postives = 636/653 (97.40%), Query Frame = 0

Query: 124 MDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGF 183
           MDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGF
Sbjct: 1   MDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGF 60

Query: 184 GPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSSTAGGNL 243
           GPPPPKWKGECQPASNFTCNNKIIGARFFR+EPLGEGDILSPRDTEGHGTHTSSTAGGNL
Sbjct: 61  GPPPPKWKGECQPASNFTCNNKIIGARFFRTEPLGEGDILSPRDTEGHGTHTSSTAGGNL 120

Query: 244 VSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGG 303
           VSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGG
Sbjct: 121 VSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGG 180

Query: 304 FIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVT 363
           FIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVT
Sbjct: 181 FIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVT 240

Query: 364 NVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEG 423
           NVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFN SLSRFCFPGSLDVNQVEG
Sbjct: 241 NVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNGSLSRFCFPGSLDVNQVEG 300

Query: 424 KIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSN 483
           KIVLCDEI DGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSN
Sbjct: 301 KIVLCDEIGDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSN 360

Query: 484 PEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLE 543
           PEATIEKSTTIED+SAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLE
Sbjct: 361 PEATIEKSTTIEDLSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLE 420

Query: 544 GDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTT-----------AF 603
           GDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTT           AF
Sbjct: 421 GDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTGNSLSLIVYVTAF 480

Query: 604 PMAPKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDES 663
           PMAPKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDES
Sbjct: 481 PMAPKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDES 540

Query: 664 NCSNAAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTV 723
           NCSN AKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVG PVSTYKAVIRAPPGLEVTV
Sbjct: 541 NCSNVAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGVPVSTYKAVIRAPPGLEVTV 600

Query: 724 RPATLSFRSLGQKMSFSVRVKANTGGNILSGSLSWEDGVHLVRSPIVAFATPS 765
           RPATLSFRSLGQKMSF+VRVKANTGGNILSGSLSWEDGVH VRSPIVAFA PS
Sbjct: 601 RPATLSFRSLGQKMSFTVRVKANTGGNILSGSLSWEDGVHQVRSPIVAFAIPS 653

BLAST of Csor.00g027330 vs. NCBI nr
Match: XP_023526089.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1249 bits (3231), Expect = 0.0
Identity = 632/653 (96.78%), Postives = 635/653 (97.24%), Query Frame = 0

Query: 124 MDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGF 183
           MDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLE+DLVIGMLDTGIWPESESFSDEGF
Sbjct: 1   MDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLETDLVIGMLDTGIWPESESFSDEGF 60

Query: 184 GPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSSTAGGNL 243
           GPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSSTAGGNL
Sbjct: 61  GPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSSTAGGNL 120

Query: 244 VSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGG 303
           VSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGG
Sbjct: 121 VSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGG 180

Query: 304 FIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVT 363
           FIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVT
Sbjct: 181 FIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVT 240

Query: 364 NVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEG 423
           NVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEG
Sbjct: 241 NVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEG 300

Query: 424 KIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSN 483
           KIVLCDEI DGE ALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSN
Sbjct: 301 KIVLCDEIGDGEGALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSN 360

Query: 484 PEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLE 543
           PEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLE
Sbjct: 361 PEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLE 420

Query: 544 GDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTT-----------AF 603
           GDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTT           AF
Sbjct: 421 GDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTGNSLSLIVYLTAF 480

Query: 604 PMAPKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDES 663
           PMAPKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDES
Sbjct: 481 PMAPKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDES 540

Query: 664 NCSNAAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTV 723
           NCS+  KTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVG PVSTYKAVIRAPPGLEVTV
Sbjct: 541 NCSDVTKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGVPVSTYKAVIRAPPGLEVTV 600

Query: 724 RPATLSFRSLGQKMSFSVRVKANTGGNILSGSLSWEDGVHLVRSPIVAFATPS 765
           RPATLSFRSLGQKMSF+VRVKA TGGNILSGSLSWEDGVHLVRSPIVAFA PS
Sbjct: 601 RPATLSFRSLGQKMSFTVRVKAKTGGNILSGSLSWEDGVHLVRSPIVAFAIPS 653

BLAST of Csor.00g027330 vs. ExPASy TrEMBL
Match: A0A6J1JLZ2 (cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111487088 PE=3 SV=1)

HSP 1 Score: 1362 bits (3525), Expect = 0.0
Identity = 683/700 (97.57%), Postives = 691/700 (98.71%), Query Frame = 0

Query: 66  MGKPSEGGFSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEARKFAKMD 125
           MGKPSEGGFSAVSQRHT+MLQQVLTNSDASKSLVYSYHRSFNGFAAWLN+EEARKFAKMD
Sbjct: 1   MGKPSEGGFSAVSQRHTHMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNEEEARKFAKMD 60

Query: 126 EVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGFGP 185
           EVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEG GP
Sbjct: 61  EVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGLGP 120

Query: 186 PPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSSTAGGNLVS 245
           PPPKWKG CQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHT+STAGGNLVS
Sbjct: 121 PPPKWKGGCQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTASTAGGNLVS 180

Query: 246 GASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGGFI 305
           GASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGGFI
Sbjct: 181 GASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGGFI 240

Query: 306 AKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVTNV 365
           AKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVTNV
Sbjct: 241 AKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVTNV 300

Query: 366 KLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEGKI 425
           KLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEGKI
Sbjct: 301 KLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEGKI 360

Query: 426 VLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSNPE 485
           VLCDEI DGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSNPE
Sbjct: 361 VLCDEIGDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSNPE 420

Query: 486 ATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLEGD 545
           ATIEKSTTIED+SAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLEGD
Sbjct: 421 ATIEKSTTIEDLSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLEGD 480

Query: 546 NRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPKLNTDAEFA 605
           NRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPKLNTDAEFA
Sbjct: 481 NRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPKLNTDAEFA 540

Query: 606 YGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSNAAKTAASDL 665
           YGAGHINP NAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCS+ AKTAASDL
Sbjct: 541 YGAGHINPANAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSDVAKTAASDL 600

Query: 666 NYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTVRPATLSFRSLGQK 725
           NYPSF LLMKSQKLNTRVYHRTVTNVG PVSTYKAVIRAPPGLEVTVRPATLSFRSLGQK
Sbjct: 601 NYPSFSLLMKSQKLNTRVYHRTVTNVGVPVSTYKAVIRAPPGLEVTVRPATLSFRSLGQK 660

Query: 726 MSFSVRVKANTGGNILSGSLSWEDGVHLVRSPIVAFATPS 765
           MSF+VRV+AN+GGNILSGSLSWEDGVHLVRSPIVAF  PS
Sbjct: 661 MSFTVRVRANSGGNILSGSLSWEDGVHLVRSPIVAFGIPS 700

BLAST of Csor.00g027330 vs. ExPASy TrEMBL
Match: A0A6J1GXX0 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458206 PE=3 SV=1)

HSP 1 Score: 1250 bits (3234), Expect = 0.0
Identity = 633/653 (96.94%), Postives = 636/653 (97.40%), Query Frame = 0

Query: 124 MDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGF 183
           MDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGF
Sbjct: 1   MDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGF 60

Query: 184 GPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSSTAGGNL 243
           GPPPPKWKGECQPASNFTCNNKIIGARFFR+EPLGEGDILSPRDTEGHGTHTSSTAGGNL
Sbjct: 61  GPPPPKWKGECQPASNFTCNNKIIGARFFRTEPLGEGDILSPRDTEGHGTHTSSTAGGNL 120

Query: 244 VSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGG 303
           VSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGG
Sbjct: 121 VSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGG 180

Query: 304 FIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVT 363
           FIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVT
Sbjct: 181 FIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVT 240

Query: 364 NVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEG 423
           NVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFN SLSRFCFPGSLDVNQVEG
Sbjct: 241 NVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNGSLSRFCFPGSLDVNQVEG 300

Query: 424 KIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSN 483
           KIVLCDEI DGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSN
Sbjct: 301 KIVLCDEIGDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSN 360

Query: 484 PEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLE 543
           PEATIEKSTTIED+SAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLE
Sbjct: 361 PEATIEKSTTIEDLSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLE 420

Query: 544 GDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTT-----------AF 603
           GDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTT           AF
Sbjct: 421 GDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTGNSLSLIVYVTAF 480

Query: 604 PMAPKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDES 663
           PMAPKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDES
Sbjct: 481 PMAPKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDES 540

Query: 664 NCSNAAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTV 723
           NCSN AKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVG PVSTYKAVIRAPPGLEVTV
Sbjct: 541 NCSNVAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGVPVSTYKAVIRAPPGLEVTV 600

Query: 724 RPATLSFRSLGQKMSFSVRVKANTGGNILSGSLSWEDGVHLVRSPIVAFATPS 765
           RPATLSFRSLGQKMSF+VRVKANTGGNILSGSLSWEDGVH VRSPIVAFA PS
Sbjct: 601 RPATLSFRSLGQKMSFTVRVKANTGGNILSGSLSWEDGVHQVRSPIVAFAIPS 653

BLAST of Csor.00g027330 vs. ExPASy TrEMBL
Match: A0A6J1CC81 (cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010157 PE=3 SV=1)

HSP 1 Score: 1156 bits (2991), Expect = 0.0
Identity = 579/752 (76.99%), Postives = 645/752 (85.77%), Query Frame = 0

Query: 16  MAFAATLLLTSQAADDRKAFHSQIFISQFHFDFILSNIIYIHIVWVYIVYMGKPSE--GG 75
           MAFA TLL+TSQA+DDRK                           VY+VYMGKPSE  GG
Sbjct: 1   MAFAYTLLVTSQASDDRK---------------------------VYVVYMGKPSEVEGG 60

Query: 76  FSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEARKFAKMDEVVSVFPS 135
           FSA S RH NMLQQVLT SDAS SLVYSYHRSF+GFAA LN +EARK A+M+ VVSVF S
Sbjct: 61  FSAASARHANMLQQVLTTSDASNSLVYSYHRSFSGFAARLNDDEARKLAEMEGVVSVFVS 120

Query: 136 EKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGFGPPPPKWKGE 195
           EKK+LHTTRSWDFMG   +A R+ LESD+++GMLDTGIWPES+SF+DEGFGPPPPKWKG+
Sbjct: 121 EKKELHTTRSWDFMGLLDEASRTRLESDVIVGMLDTGIWPESKSFTDEGFGPPPPKWKGK 180

Query: 196 CQPASNFTCNNKIIGARFFRSEPL-GEGDILSPRDTEGHGTHTSSTAGGNLVSGASLFGL 255
           CQ +SNFTCNNK+IGARF+RS P+ G GDILSPRDTEGHGTHTSSTA GNLV+ ASLFGL
Sbjct: 181 CQSSSNFTCNNKLIGARFYRSNPVEGGGDILSPRDTEGHGTHTSSTAAGNLVAQASLFGL 240

Query: 256 GLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGGFIAKDYFND 315
           GLGT+RGG PSAR+AVYKICWSDGC DADILAAFD+AIADGVD+IS+SVGG I ++YF D
Sbjct: 241 GLGTSRGGVPSARVAVYKICWSDGCSDADILAAFDDAIADGVDVISISVGGSIPREYFKD 300

Query: 316 SIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVTNVKLGNGES 375
           SIAIGAFHAMKNGILTSNS GN+GP   +I+NVSPWSLSVAASTIDRKFVT VKLGNG++
Sbjct: 301 SIAIGAFHAMKNGILTSNSAGNTGPGSATISNVSPWSLSVAASTIDRKFVTKVKLGNGKA 360

Query: 376 SQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEGKIVLCDEIS 435
            +GISVNTFQL DK+ PLIYAGDAPN+TAGFN SLSRFCFPGSLD+N V+GKIV CDEI 
Sbjct: 361 FEGISVNTFQLEDKMFPLIYAGDAPNSTAGFNGSLSRFCFPGSLDMNLVQGKIVFCDEIG 420

Query: 436 DGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSNPEATIEKST 495
           DG  ALSSGA+GTIMQD    D AF+FPLPAS LDLNAG+ VFQYLRSTSNPEA IEKST
Sbjct: 421 DGTTALSSGAIGTIMQDANFLDVAFVFPLPASKLDLNAGSKVFQYLRSTSNPEANIEKST 480

Query: 496 TIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLEGDNRVSPFN 555
           TI+D+SAP VVSFSSRGPN ITLDILKPDL APGVDI+ASWSE  T+  L GDNRV PFN
Sbjct: 481 TIDDLSAPFVVSFSSRGPNQITLDILKPDLTAPGVDILASWSEDATVTSLAGDNRVVPFN 540

Query: 556 IISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPKLNTDAEFAYGAGHIN 615
           IISGTSM+CPHATG AAYVKSFHPTWSPAAIKSALMTTAFPM PKLN DAEFAYGAGH+N
Sbjct: 541 IISGTSMSCPHATGTAAYVKSFHPTWSPAAIKSALMTTAFPMTPKLNADAEFAYGAGHLN 600

Query: 616 PVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSNAAKTAASDLNYPSFGL 675
           PVNAINPGLVYDA EIDYIKFLCGQG+ST NL LVTGD+SNCS+  KTAASDLNYPSF L
Sbjct: 601 PVNAINPGLVYDATEIDYIKFLCGQGFSTNNLSLVTGDQSNCSDVRKTAASDLNYPSFAL 660

Query: 676 LMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTVRPATLSFRSLGQKMSFSVRV 735
            +K+++L +RV+HRTVTNVG PVSTYKA+I+A PGL+VTVRPATLSF SLGQK+SF+VR+
Sbjct: 661 SIKTRRLVSRVFHRTVTNVGLPVSTYKAIIQAAPGLKVTVRPATLSFSSLGQKISFTVRI 720

Query: 736 KANTGGNILSGSLSWEDGVHLVRSPIVAFATP 764
           KA T G ++SGSL+W+DGVHLVRSPIV F  P
Sbjct: 721 KAKTAGKLISGSLTWDDGVHLVRSPIVTFVNP 725

BLAST of Csor.00g027330 vs. ExPASy TrEMBL
Match: A0A5A7UDF9 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001080 PE=3 SV=1)

HSP 1 Score: 1123 bits (2905), Expect = 0.0
Identity = 565/705 (80.14%), Postives = 628/705 (89.08%), Query Frame = 0

Query: 66  MGKPSEGGFSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEARKFAKMD 125
           MGKPS GG  A S+ HTNMLQQVLT SDASKSLVYSYHRSF+GFAA LN +EARK A+MD
Sbjct: 1   MGKPSGGGLLAASELHTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNDDEARKLAEMD 60

Query: 126 EVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGFGP 185
            VVSVFPSEKKQLHTTRSWDFMGFFQ+A R  LESDL+IGMLDTGIWPES+SFSDEGFGP
Sbjct: 61  GVVSVFPSEKKQLHTTRSWDFMGFFQEAPRKRLESDLIIGMLDTGIWPESKSFSDEGFGP 120

Query: 186 PPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSSTAGGNLVS 245
           PP KWKGEC  A NFTCNNKIIGARFFRSEP+  GD  SPRDTEGHGTHTSSTAGGNLV+
Sbjct: 121 PPSKWKGEC--AHNFTCNNKIIGARFFRSEPV-IGDSPSPRDTEGHGTHTSSTAGGNLVT 180

Query: 246 GASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGGFI 305
            A+LFGL  GT+RGG PSARIAVYKICWS+GC DADILAAFD+AIADGVDIIS+SVGGF 
Sbjct: 181 DANLFGLAAGTSRGGAPSARIAVYKICWSNGCPDADILAAFDHAIADGVDIISISVGGFG 240

Query: 306 AKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVTNV 365
           A +Y +D IAIGAFHAMKNGILTSNSGGNSGP+LGS++NVSPWSLSVAASTIDRKFVT V
Sbjct: 241 ASNYLDDPIAIGAFHAMKNGILTSNSGGNSGPNLGSLSNVSPWSLSVAASTIDRKFVTKV 300

Query: 366 KLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEGKI 425
            LGNGES QGISVNTF+LGDKL PLI+AGDAPNTTAGFN S SR CFPGSLDV++V+GKI
Sbjct: 301 TLGNGESIQGISVNTFELGDKLFPLIHAGDAPNTTAGFNGSTSRLCFPGSLDVDKVQGKI 360

Query: 426 VLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSNPE 485
           V+CD IS G+   SSGAVGT+M D G QD AFLFP P S++  + G ++FQYLRS SNPE
Sbjct: 361 VICDLISGGDVTQSSGAVGTVMLDSGFQDVAFLFPQPVSLISFHTGKSLFQYLRSNSNPE 420

Query: 486 ATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLEGD 545
           A IEKSTTIED+SAPSVVSFSSRGPN+ITLDILKPDLAAPGVDI+ASWSE T+I G+EGD
Sbjct: 421 AIIEKSTTIEDLSAPSVVSFSSRGPNVITLDILKPDLAAPGVDIIASWSEATSITGVEGD 480

Query: 546 NRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTA--FPMAPKLNTDAE 605
            R++PFNIISGTSM+CPHATGAAAYVKSFHP+WSPAAIKSALMT+   FPM+PKLNTDAE
Sbjct: 481 KRIAPFNIISGTSMSCPHATGAAAYVKSFHPSWSPAAIKSALMTSGNTFPMSPKLNTDAE 540

Query: 606 FAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSNAAKTAAS 665
            AYGAGH+NPVNAINPGLVYDAEE+DYIKFLCGQGYST++LRLV+GD SNCS+  KTAAS
Sbjct: 541 LAYGAGHLNPVNAINPGLVYDAEELDYIKFLCGQGYSTKDLRLVSGDHSNCSDVTKTAAS 600

Query: 666 DLNYPSFGLLMKS--QKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTVRPATLSFRS 725
           DLNYPSFGL++ S  Q+L +RVYHRTVTNVG PVSTYKAVI+APPGL+VTVRPATLSFRS
Sbjct: 601 DLNYPSFGLVINSTSQRLISRVYHRTVTNVGLPVSTYKAVIKAPPGLKVTVRPATLSFRS 660

Query: 726 LGQKMSFSV--RVKANTGGNILSGSLSWEDGVHLVRSPIVAFATP 764
           LGQK+SF+V  R KA+  G ++SGSL+W+DGVHLVRSPIV+F  P
Sbjct: 661 LGQKISFTVTVRAKADVAGKVVSGSLTWDDGVHLVRSPIVSFVIP 702

BLAST of Csor.00g027330 vs. ExPASy TrEMBL
Match: A0A1S3CMK1 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103502667 PE=3 SV=1)

HSP 1 Score: 1092 bits (2825), Expect = 0.0
Identity = 547/677 (80.80%), Postives = 609/677 (89.96%), Query Frame = 0

Query: 92  SDASKSLVYSYHRSFNGFAAWLNQEEARKFAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQ 151
           SDASKSLVYSYHRSF+GFAA LN +EARK A+MD VVSVFPSEKKQLHTTRSWDFMGFFQ
Sbjct: 11  SDASKSLVYSYHRSFSGFAARLNDDEARKLAEMDGVVSVFPSEKKQLHTTRSWDFMGFFQ 70

Query: 152 QARRSSLESDLVIGMLDTGIWPESESFSDEGFGPPPPKWKGECQPASNFTCNNKIIGARF 211
           +A R  LESDL+IGMLDTGIWPES+SFSDEGFGPPP KWKGEC  A NFTCNNKIIGARF
Sbjct: 71  EAPRKRLESDLIIGMLDTGIWPESKSFSDEGFGPPPSKWKGEC--AHNFTCNNKIIGARF 130

Query: 212 FRSEPLGEGDILSPRDTEGHGTHTSSTAGGNLVSGASLFGLGLGTARGGTPSARIAVYKI 271
           FRSEP+  GD  SPRDTEGHGTHTSSTAGGNLV+ A+LFGL  GT+RGG PSARIAVYKI
Sbjct: 131 FRSEPV-IGDSPSPRDTEGHGTHTSSTAGGNLVTDANLFGLAAGTSRGGAPSARIAVYKI 190

Query: 272 CWSDGCFDADILAAFDNAIADGVDIISLSVGGFIAKDYFNDSIAIGAFHAMKNGILTSNS 331
           CWS+GC DADILAAFD+AIADGVDIIS+SVGGF A +Y +D IAIGAFHAMKNGILTSNS
Sbjct: 191 CWSNGCPDADILAAFDHAIADGVDIISISVGGFGASNYLDDPIAIGAFHAMKNGILTSNS 250

Query: 332 GGNSGPSLGSITNVSPWSLSVAASTIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLI 391
           GGNSGP+LGS++NVSPWSLSVAASTIDRKFVT V LGNGES QGISVNTF+LGDKL PLI
Sbjct: 251 GGNSGPNLGSLSNVSPWSLSVAASTIDRKFVTKVTLGNGESIQGISVNTFELGDKLFPLI 310

Query: 392 YAGDAPNTTAGFNESLSRFCFPGSLDVNQVEGKIVLCDEISDGEAALSSGAVGTIMQDGG 451
           +AGDAPNTTAGFN S SR CFPGSLDV++V+GKIV+CD IS G+   SSGAVGT+M D G
Sbjct: 311 HAGDAPNTTAGFNGSTSRLCFPGSLDVDKVQGKIVICDLISGGDVTQSSGAVGTVMLDSG 370

Query: 452 LQDAAFLFPLPASVLDLNAGNNVFQYLRSTSNPEATIEKSTTIEDMSAPSVVSFSSRGPN 511
            QD AFLFP P S++  + G ++FQYLRS SNPEA IEKSTTIED+SAPSVVSFSSRGPN
Sbjct: 371 FQDVAFLFPQPVSLISFHTGKSLFQYLRSNSNPEAIIEKSTTIEDLSAPSVVSFSSRGPN 430

Query: 512 LITLDILKPDLAAPGVDIVASWSEGTTIGGLEGDNRVSPFNIISGTSMACPHATGAAAYV 571
           +ITLDILKPDLAAPGVDI+ASWSE T+I G+EGD R++PFNIISGTSM+CPHATGAAAYV
Sbjct: 431 VITLDILKPDLAAPGVDIIASWSEATSITGVEGDKRIAPFNIISGTSMSCPHATGAAAYV 490

Query: 572 KSFHPTWSPAAIKSALMTTAFPMAPKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYI 631
           KSFHP+WSPAAIKSALMT+AFPM+PKLNTDAE AYGAGH+NPVNAINPGLVYDAEE+DYI
Sbjct: 491 KSFHPSWSPAAIKSALMTSAFPMSPKLNTDAELAYGAGHLNPVNAINPGLVYDAEELDYI 550

Query: 632 KFLCGQGYSTQNLRLVTGDESNCSNAAKTAASDLNYPSFGLLMKS--QKLNTRVYHRTVT 691
           KFLCGQGYST++LRLV+GD SNCS+  KTAASDLNYPSFGL++ S  Q+L +RVYHRTVT
Sbjct: 551 KFLCGQGYSTKDLRLVSGDHSNCSDVTKTAASDLNYPSFGLVINSTSQRLISRVYHRTVT 610

Query: 692 NVGEPVSTYKAVIRAPPGLEVTVRPATLSFRSLGQKMSFSV--RVKANTGGNILSGSLSW 751
           NVG PVSTYKAVI+APPGL+VTVRPATLSFRSLGQK+SF+V  R KA+  G ++SGSL+W
Sbjct: 611 NVGLPVSTYKAVIKAPPGLKVTVRPATLSFRSLGQKISFTVTVRAKADVAGKVVSGSLTW 670

Query: 752 EDGVHLVRSPIVAFATP 764
           +DGVHLVRSPIV+F  P
Sbjct: 671 DDGVHLVRSPIVSFVIP 684

BLAST of Csor.00g027330 vs. TAIR 10
Match: AT4G00230.1 (xylem serine peptidase 1 )

HSP 1 Score: 650.6 bits (1677), Expect = 1.6e-186
Identity = 352/716 (49.16%), Postives = 486/716 (67.88%), Query Frame = 0

Query: 62  YIVYMGKPSEGGFSAVSQRHTNMLQQV-LTNSDASKSLVYSYHRSFNGFAAWLNQEEARK 121
           YI+Y+G   +     + + H N+L  + ++  +A +  VYSY ++FN FAA L+  EA+K
Sbjct: 38  YIIYLGDRPDNTEETI-KTHINLLSSLNISQEEAKERKVYSYTKAFNAFAAKLSPHEAKK 97

Query: 122 FAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRS-SLESDLVIGMLDTGIWPESESFS 181
             +M+EVVSV  ++ ++LHTT+SWDF+G    A+R    E D++IG+LDTGI P+SESF 
Sbjct: 98  MMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRHLKAERDVIIGVLDTGITPDSESFL 157

Query: 182 DEGFGPPPPKWKGECQPASNFT-CNNKIIGARFFRSE-PLGEGDILSPRDTEGHGTHTSS 241
           D G GPPP KWKG C P  NFT CNNKIIGA++F+ +  +  G++ SP D +GHGTHTSS
Sbjct: 158 DHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKHDGNVPAGEVRSPIDIDGHGTHTSS 217

Query: 242 TAGGNLVSGASLFGLGLGTARGGTPSARIAVYKICWS-DGCFDADILAAFDNAIADGVDI 301
           T  G LV+ ASL+G+  GTARG  PSAR+A+YK+CW+  GC D DILA F+ AI DGV+I
Sbjct: 218 TVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCADMDILAGFEAAIHDGVEI 277

Query: 302 ISLSVGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAAST 361
           IS+S+GG IA DY +DSI++G+FHAM+ GILT  S GN GPS G++TN  PW L+VAAS 
Sbjct: 278 ISISIGGPIA-DYSSDSISVGSFHAMRKGILTVASAGNDGPSSGTVTNHEPWILTVAASG 337

Query: 362 IDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSL 421
           IDR F + + LGNG+S  G+ ++ F    K  PL+   DA   T   ++ L+R+CF  SL
Sbjct: 338 IDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVSGVDAAKNTD--DKYLARYCFSDSL 397

Query: 422 DVNQVEGKIVLCDEISDG-EAALSS-GAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNV 481
           D  +V+GK+++C     G E+ + S G  G I+      D A +F  PA+ ++ + G+ +
Sbjct: 398 DRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQYLDNAQIFMAPATSVNSSVGDII 457

Query: 482 FQYLRSTSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWS 541
           ++Y+ ST +  A I+K+  +  + AP V SFSSRGPN  ++ +LKPD+AAPG+DI+A+++
Sbjct: 458 YRYINSTRSASAVIQKTRQV-TIPAPFVASFSSRGPNPGSIRLLKPDIAAPGIDILAAFT 517

Query: 542 EGTTIGGLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPM 601
              ++ GL+GD + S F I+SGTSMACPH  G AAYVKSFHP W+PAAIKSA++T+A P+
Sbjct: 518 LKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKSFHPDWTPAAIKSAIITSAKPI 577

Query: 602 APKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDES-N 661
           + ++N DAEFAYG G INP  A +PGLVYD ++I Y++FLCG+GY+   L  + G  S +
Sbjct: 578 SRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDISYVQFLCGEGYNATTLAPLVGTRSVS 637

Query: 662 CSNAAKTAASD-LNYPSFGLLMKSQKLNT-RVYHRTVTNVGEPVSTYKAVIRAPPGLEVT 721
           CS+       D LNYP+  L ++S K +T  V+ R VTNVG P S Y A +RAP G+E+T
Sbjct: 638 CSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFRRRVTNVGPPSSVYTATVRAPKGVEIT 697

Query: 722 VRPATLSFRSLGQKMSFSVRVKAN--TGGNILSGSLSWEDGVHLVRSPIVAFATPS 766
           V P +LSF    QK SF V VKA   T G I+SG L W+   H VRSPIV ++  S
Sbjct: 698 VEPQSLSFSKASQKRSFKVVVKAKQMTPGKIVSGLLVWKSPRHSVRSPIVIYSPTS 748

BLAST of Csor.00g027330 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 649.8 bits (1675), Expect = 2.7e-186
Identity = 349/703 (49.64%), Postives = 461/703 (65.58%), Query Frame = 0

Query: 66  MGKPSEGGFSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEARKFAKMD 125
           MG   E  +S  S  H ++LQ+++    AS  LV SY RSFNGFAA L+Q E++K   M 
Sbjct: 1   MGTLPEIKYSPPS-HHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMK 60

Query: 126 EVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSL-ESDLVIGMLDTGIWPESESFSDEGFG 185
           EVVSVFPS+  +L TTRSWDF+GF ++ARR S+ ESD+++G++D+GIWPESESF DEGFG
Sbjct: 61  EVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFG 120

Query: 186 PPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSSTAGGNLV 245
           PPP KWKG C+    F CNNK+IGARF+           S RD EGHGTHT+STA GN V
Sbjct: 121 PPPKKWKGSCKGGLKFACNNKLIGARFYNK------FADSARDEEGHGTHTASTAAGNAV 180

Query: 246 SGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGGF 305
             AS +GL  GTARGG PSARIA YK+C+ + C D DILAAFD+AIADGVD+IS+S+   
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240

Query: 306 IAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVTN 365
              +  N S+AIG+FHAM  GI+T+ S GN+GP  GS+ NVSPW ++VAAS  DR+F+  
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 366 VKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEGK 425
           V LGNG++  GISVNTF L     P++Y     N +   +++ + +C  G +D   V+GK
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDSELVKGK 360

Query: 426 IVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSNP 485
           IVLCD+      A  +GA+G I+Q+  L D+AF+ P PAS L      ++  Y+ S   P
Sbjct: 361 IVLCDDFLGYREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPP 420

Query: 486 EATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGL-- 545
           +A I ++  I D  AP V SFSSRGP+ +  ++LKPD++APG++I+A++S   +      
Sbjct: 421 QAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLN 480

Query: 546 EGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPKLNTDA 605
             D R   ++++SGTSMACPH  G AAYVKSFHP WSP+AIKSA+MTTA PM  K N + 
Sbjct: 481 PEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQ 540

Query: 606 EFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSNAAKTAA 665
           EFAYG+G INP  A +PGLVY+ E  DY+K LC +G+ +  L   +G    CS   +T  
Sbjct: 541 EFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSE--RTEV 600

Query: 666 SDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKA-VIRAPPGLEVTVRPATLSFRS 725
            DLNYP+    + S       + RTVTNVG P STYKA V+   P L++++ P  L F  
Sbjct: 601 KDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGF 660

Query: 726 LGQKMSFSVRV--KANTGGNILSGSLSWEDGVHLVRSPIVAFA 763
           L +K SF V +  K    G+ +S S+ W DG H VRSPIVA++
Sbjct: 661 LEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 690

BLAST of Csor.00g027330 vs. TAIR 10
Match: AT3G46840.1 (Subtilase family protein )

HSP 1 Score: 632.9 bits (1631), Expect = 3.4e-181
Identity = 357/714 (50.00%), Postives = 468/714 (65.55%), Query Frame = 0

Query: 62  YIVYMGK-PSEGGFSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEARK 121
           YIVYMG  P+   +  +S  HT++LQ V   S     LV +Y RSFNGFAA L + E   
Sbjct: 35  YIVYMGALPARVDYMPMS-HHTSILQDVTGESSIEDRLVRNYKRSFNGFAARLTKSEREI 94

Query: 122 FAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQQ---ARRSSLESDLVIGMLDTGIWPESES 181
            A MDEVVSVFP++K +L TT SW+FMG  +     R + +ESD +IG++D+GI+PES+S
Sbjct: 95  LASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRTKRNTIIESDTIIGVIDSGIYPESDS 154

Query: 182 FSDEGFGPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTSS 241
           FS +GFGPPP KWKG C+   NFT NNK+IGAR++   P  EG   S RD  GHG+HT+S
Sbjct: 155 FSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYY--TPKLEGFPESARDYMGHGSHTAS 214

Query: 242 TAGGNLVSGASLFGLGLGTARGGTPSARIAVYKIC--WSDGCFDADILAAFDNAIADGVD 301
           TA GN V   S +GLG GTARGG P+ARIAVYK+C    DGC    ILAAFD+AIAD VD
Sbjct: 215 TAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDPGVDGCTTDGILAAFDDAIADKVD 274

Query: 302 IISLSVGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAAS 361
           II++S+GG  +  +  D IAIGAFHAM  GIL  NS GNSGP   ++ +++PW  +VAAS
Sbjct: 275 IITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSAGNSGPEPSTVASIAPWMFTVAAS 334

Query: 362 TIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGS 421
             +R FVT V LGNG+ + G SVN+F L  K  PL+Y G + +++ G   + + FC PG 
Sbjct: 335 NTNRAFVTKVVLGNGK-TVGRSVNSFDLNGKKYPLVY-GKSASSSCG--AASAGFCSPGC 394

Query: 422 LDVNQVEGKIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVF 481
           LD  +V+GKIVLCD   + + A + GA+ +I++     D A +F  P SVL  +  N V 
Sbjct: 395 LDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVR-SHRTDVASIFSFPVSVLLEDDYNTVL 454

Query: 482 QYLRSTSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSE 541
            Y+ ST NP+A + KS TI +  AP V S+ SRGPN I  DILKPD+ APG +IVA++S 
Sbjct: 455 SYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIPDILKPDITAPGSEIVAAYSP 514

Query: 542 GTTIGGLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPM- 601
                    D R   +++ +GTSM+CPH  G AAY+KSFHP WSP+ I+SA+MTTA+PM 
Sbjct: 515 DAPPS--ISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFHPRWSPSMIQSAIMTTAWPMN 574

Query: 602 --APKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDES 661
                 N  AEFAYGAGH++P+ AI+PGLVY+A + D+I FLCG  Y+ +NLRL++GD S
Sbjct: 575 ASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIAFLCGLNYTAKNLRLISGDSS 634

Query: 662 NCS-NAAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVT 721
           +C+    K+   +LNYPS    + + K    ++ RTVTNVG P +TYKA +     L+V 
Sbjct: 635 SCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNVGRPNATYKAKV-VGSKLKVK 694

Query: 722 VRPATLSFRSLGQKMSFSVRVK--ANTGGNILSGSLSWEDGVHLVRSPIVAFAT 764
           V PA LS +SL +K SF+V          N++S  L W DGVH VRSPIV +AT
Sbjct: 695 VVPAVLSLKSLYEKKSFTVTASGAGPKAENLVSAQLIWSDGVHFVRSPIVVYAT 737

BLAST of Csor.00g027330 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 630.2 bits (1624), Expect = 2.2e-180
Identity = 355/714 (49.72%), Postives = 464/714 (64.99%), Query Frame = 0

Query: 61  VYIVYMGK-PSEGGFSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEAR 120
           VYIVYMG  PS   +  +S  HT++LQ V   S     LV +Y RSFNGFAA L + E  
Sbjct: 33  VYIVYMGALPSRVDYMPMS-HHTSILQDVTGESSIQDRLVRNYKRSFNGFAARLTESERE 92

Query: 121 KFAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQ---QARRSSLESDLVIGMLDTGIWPESE 180
             A MDEVVSVFPS+   L TT SW+FMG  +     R   +ESD +IG++D+GI+PES+
Sbjct: 93  ILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKRNPLIESDTIIGVIDSGIYPESD 152

Query: 181 SFSDEGFGPPPPKWKGECQPASNFTCNNKIIGARFFRSEPLGEGDILSPRDTEGHGTHTS 240
           SFS +GFGPPP KWKG C+  +NFTCNNK+IGAR++   P  EG   S RD  GHG+HT+
Sbjct: 153 SFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYY--TPKLEGFPESARDNTGHGSHTA 212

Query: 241 STAGGNLVSGASLFGLGLGTARGGTPSARIAVYKICWSD--GCFDADILAAFDNAIADGV 300
           S A GN V   S +GLG GT RGG P+ARIAVYK+C      C    ILAAFD+AIAD V
Sbjct: 213 SIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGVIRCTSDGILAAFDDAIADKV 272

Query: 301 DIISLSVGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAA 360
           DII++S+G      +  D++AIGAFHAM  GILT N  GN+GP   +I +++PW  +VAA
Sbjct: 273 DIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGNNGPERRTIVSMAPWLFTVAA 332

Query: 361 STIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPG 420
           S ++R F+T V LGNG++  G SVN+F L  K  PL+Y   A   ++  + S + FC PG
Sbjct: 333 SNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVYGKSA---SSRCDASSAGFCSPG 392

Query: 421 SLDVNQVEGKIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNV 480
            LD  +V+GKIVLCD   +   A + GAV +I+++   +DAA +F  P SVL  +  N V
Sbjct: 393 CLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRN-PYEDAASVFSFPVSVLSEDDYNIV 452

Query: 481 FQYLRSTSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWS 540
             Y+ ST NP+A + KS TI +  AP V S+SSRGPN +  DILKPD+ APG +I+A++S
Sbjct: 453 LSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAYS 512

Query: 541 EGTTIGGLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPM 600
               +   E D R   + +ISGTSM+CPH  G AAY+K+FHP WSP+ I+SA+MTTA+PM
Sbjct: 513 --PYVPPSESDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPM 572

Query: 601 ---APKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDE 660
                  N  AEFAYGAGH++P+ AI+PGLVY+A + D+I FLCG  Y+ + LRL++GD 
Sbjct: 573 NASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDS 632

Query: 661 SNCS-NAAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEV 720
           S+C+    K+   +LNYPS    +   K     + RTVTNVG P +TYKA +     L+V
Sbjct: 633 SSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKV-VGSKLKV 692

Query: 721 TVRPATLSFRSLGQKMSFSVRVK--ANTGGNILSGSLSWEDGVHLVRSPIVAFA 763
            V PA LS +SL +K SF+V V        N++S  L W DGVH VRSPIV +A
Sbjct: 693 KVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVVYA 736

BLAST of Csor.00g027330 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 622.9 bits (1605), Expect = 3.5e-178
Identity = 356/729 (48.83%), Postives = 479/729 (65.71%), Query Frame = 0

Query: 62  YIVYMGKPSEGGFSAVSQRHTNMLQQVLTNSDASKSL-VYSYHRSFNGFAAWLNQEEARK 121
           YIVYMG+ +E      ++ H N+L  V+ +   ++ L +YSY ++ NGF A L   EA K
Sbjct: 35  YIVYMGEATENSLVEAAENHHNLLMTVIGDESKARELKIYSYGKNINGFVARLFPHEAEK 94

Query: 122 FAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSS--LESDLVIGMLDTGIWPESESF 181
            ++ + VVSVF + ++QLHTTRSWDF+G  +   + S  +ES++++G+LDTGI  ES SF
Sbjct: 95  LSREEGVVSVFKNTQRQLHTTRSWDFLGLVESKYKRSVGIESNIIVGVLDTGIDVESPSF 154

Query: 182 SDEGFGPPPPKWKGECQPASNFT-CNNKIIGARFF--RSEPLGEGDILSPRDTEGHGTHT 241
           +D+G GPPP KWKG+C   +NFT CNNK+IGA++F  +SE L +G+  +  D +GHGTHT
Sbjct: 155 NDKGVGPPPAKWKGKCVTGNNFTRCNNKVIGAKYFHIQSEGLPDGEGDTAADHDGHGTHT 214

Query: 242 SSTAGGNLVSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVD 301
           SST  G  VS ASLFG+  GTARGG PSARIA YK+CW  GC D D+LAAFD AI+DGVD
Sbjct: 215 SSTIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCWDSGCTDMDMLAAFDEAISDGVD 274

Query: 302 IISLSVGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAAS 361
           IIS+S+GG  +  +F D IAIGAFHAMK GILT+ S GN+GP L +++N++PW ++VAA+
Sbjct: 275 IISISIGG-ASLPFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLFTVSNLAPWVMTVAAN 334

Query: 362 TIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGS 421
           ++DRKF T VKLGNG ++ GIS+N F    K+ PL     A N +AG     S  C PG+
Sbjct: 335 SLDRKFETVVKLGNGLTASGISLNGFNPRKKMYPLTSGSLASNLSAGGYGEPST-CEPGT 394

Query: 422 LDVNQVEGKIVLC----DEISDG-----EAALSSGAVGTIMQDGGLQDAAFLFPLPASVL 481
           L  ++V GK+V C    +E  +G         S    G I+Q     D A    +  S +
Sbjct: 395 LGEDKVMGKVVYCEAGREEGGNGGQGQDHVVRSLKGAGVIVQLLEPTDMATSTLIAGSYV 454

Query: 482 DLNAGNNVFQYLRSTSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPG 541
               G  + +Y+ ST NP+A I K+ T + M APS+ SFS+RGP  I+ +ILKPD++APG
Sbjct: 455 FFEDGTKITEYINSTKNPQAVIFKTKTTK-MLAPSISSFSARGPQRISPNILKPDISAPG 514

Query: 542 VDIVASWSEGTTIGGLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSA 601
           ++I+A++S+  ++ G   DNR + F+I+SGTSMACPHA  AAAYVKSFHP WSPAAIKSA
Sbjct: 515 LNILAAYSKLASVTGYPDDNRRTLFSIMSGTSMACPHAAAAAAYVKSFHPDWSPAAIKSA 574

Query: 602 LMTTAFPMAPKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRL 661
           LMTTA PM  K N +AE +YG+G INP  AI+PGLVYD  E  Y++FLC +GY++ ++ L
Sbjct: 575 LMTTATPMRIKGN-EAELSYGSGQINPRRAIHPGLVYDITEDAYLRFLCKEGYNSTSIGL 634

Query: 662 VTGDES--------NCSNAAKTAASD-LNYPSFGLLMKSQKLN-TRVYHRTVTNVGEPVS 721
           +TGD S        NC N  +   SD LNYPS    + S +   + V++RTVTNVG   S
Sbjct: 635 LTGDNSNNTTKKEYNCENIKRGLGSDGLNYPSLHKQVNSTEAKVSEVFYRTVTNVGYGPS 694

Query: 722 TYKAVIRAPPGLEVTVRPATLSFRSLGQKMSFSVRVKA---NTGGNILSGSLSWEDG-VH 762
           TY A + AP GL V V P  +SF    +K +F V +      T   I+S S+ W+D   H
Sbjct: 695 TYVARVWAPKGLRVEVVPKVMSFERPKEKRNFKVVIDGVWDETMKGIVSASVEWDDSRGH 754

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q395479.5e-22156.41Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1[more]
Q9FIF88.2e-18850.00Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... [more]
Q9LLL82.2e-18549.16Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2... [more]
F4JA914.8e-18050.00Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 S... [more]
Q9STF73.1e-17949.72Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S... [more]
Match NameE-valueIdentityDescription
KAG6600966.10.0100.00hypothetical protein SDJN03_06199, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7031587.10.098.73hypothetical protein SDJN02_05628, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022990086.10.097.57cucumisin-like [Cucurbita maxima][more]
XP_022956485.10.096.94cucumisin-like [Cucurbita moschata][more]
XP_023526089.10.096.78cucumisin-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1JLZ20.097.57cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111487088 PE=3 SV=1[more]
A0A6J1GXX00.096.94cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458206 PE=3 SV=1[more]
A0A6J1CC810.076.99cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010157 PE=3 SV=1[more]
A0A5A7UDF90.080.14Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G00108... [more]
A0A1S3CMK10.080.80cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103502667 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G00230.11.6e-18649.16xylem serine peptidase 1 [more]
AT5G59190.12.7e-18649.64subtilase family protein [more]
AT3G46840.13.4e-18150.00Subtilase family protein [more]
AT3G46850.12.2e-18049.72Subtilase family protein [more]
AT5G03620.13.5e-17848.83Subtilisin-like serine endopeptidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 227..240
score: 39.54
coord: 159..178
score: 25.94
coord: 555..571
score: 55.04
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 61..139
e-value: 5.3E-15
score: 55.9
NoneNo IPR availableGENE3D2.60.40.2310coord: 634..762
e-value: 1.7E-42
score: 146.3
NoneNo IPR availableGENE3D3.50.30.30coord: 358..495
e-value: 1.3E-185
score: 619.6
NoneNo IPR availablePANTHERPTHR10795:SF649PUTATIVE-RELATEDcoord: 49..762
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 143..617
score: 24.654531
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 364..488
e-value: 1.70768E-18
score: 80.1503
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 59..139
e-value: 1.1E-25
score: 91.9
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 664..759
e-value: 6.1E-28
score: 96.9
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 161..630
e-value: 1.3E-185
score: 619.6
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 143..618
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 160..609
e-value: 1.3E-41
score: 142.8
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 49..762
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 556..566
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 136..591
e-value: 1.61742E-129
score: 385.798

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g027330.m01Csor.00g027330.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity