Csor.00g023910 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g023910
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionDNA-directed RNA polymerases II and V subunit 8A-like
LocationCsor_Chr04: 4128375 .. 4130097 (+)
RNA-Seq ExpressionCsor.00g023910
SyntenyCsor.00g023910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGCTTCTATTTGATGATATTTTCAAGGTCAAGTGCGTCAACCCAGATGGTAAAAAGTATGATAAAGGTACTTTCATGAAGCTTGTAACTGCTATCTCATTCTTTTAATTCACATTTTATGATCTTTTAACTGTCTATGAGTCCTTTTGTAGACAGTAACTTCTGCACCATCTTGACTTTTATGTGAATGATTTGATATGGTGTTTATTGGGTGCATAAAATTGTTCTTATAGTTTCTAGAATTGAAGCACGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGTGACAAGATTCTGATGGTCCTGTCTCCTACGCTGAATTATGATGGAGCTCCTGTTACCGGCTATAGTGGTCAAGTAATATTTCTCCCCTCAATGTTTAATTGAATTTCATACAATCTTGGTGCTATCTGGTAGTCGTTTCATGTCTCTTCATTGTTTAAAAGAACACTCGGAAACACCTTGCTGCTAGATATCTGCATCCATATGTATCTTATAGATTCTAAAAGAAACGACCAATGATTTCATAATCTTTCTCTGATGCTGAAATTTTGGAGAACGGATTTTACCTAACGATCTATAATAGCCCAAGCTCACTGCTAGCAGACATTGTCCTCTTCGAGTTTTCCCTTTCGAGCTTCCAAACATGTCTCCTAGGGAGAGGTTTCCACACCCTTATAAGGAATATTTTGTTCTCCTCTCCAACTGATGTGGAGTCTCACAATCCATCCCCCTTCGAGGCCCGATGTCCTTGCTGGCACTCGATCAACTCTCCAATCGATGTAGGATCTCACAATCCACCTCTCTTCGAGGTCCAACGTCCTCGCTGCCACACTGGCTGATGTCCACCCCCTTCGGGGCTTAGTCTCCTCACTGGCACACCGCCCGGTGTCCATCCCCCTTGGGGCTCAGTGTTCTCGCTGGCACACCGTCCGGTATTTGGCTTTGACACCATTTGTAATGGCCCAGACCATCAATAGCAAATATTGTCCTCTTTGGGCTTTTCCTTTTGGACTTCTCCTCAAATTTTTAAAACCCATCTGCTAAAGAGAGGTTTTCACGCCCTTATAAGAAATGCTCCGTTCCCCTCTACAATCGACGTGGGATCTCACAGTTTCTATAAACTTTTCCTAAAGTAAAAAACAGAACAGTGATTATAAGCAAACAGCTTTATGTTCTAAAAACAAAATTATGCAAATGTTTCTTAGTATGGGAAGTGCCGAGTAGGCACTAATATCAAGCACAAACCCGTAACTTCAGCCGTGAAGATTGTGAACATAATTGAAATTTGTAGTAGCAGGAGTAATATTTTTGAACTAAACTTAAGTTGTTGCAGAAAGGGAAGAAATCACTAGCAGATAAGTTCGAGTATATCATGCATGGGAAGCTGTACAAACTCGCAGATGAAGGCTCAGGATCAGACTTGAAAGTGTAAGTGAATTGCCATGATTTTGCTCACCCATTTCTGCTGCTGATTTTCCTAAAAGTCTAAGTGGAAGTACTTTATGCATGATTCTAACTCGATTCATAAAACATATTTTGATGTCGGAATTGGGTGTATTTTACAGAGAGGTTTATGCATCTTTTGGAGGACTTCAAATGATGTTGAAGGGAGATCCCCTCCACTGTGCCAAATTCAGGGTAGACCAGAACATGTTCTTGCTCCTCCGAAAGCTCACATGA

mRNA sequence

ATGGCGGAGCTTCTATTTGATGATATTTTCAAGGTCAAGTGCGTCAACCCAGATGGTAAAAAGTATGATAAAGTTTCTAGAATTGAAGCACGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGTGACAAGATTCTGATGGTCCTGTCTCCTACGCTGAATTATGATGGAGCTCCTGTTACCGGCTATAGTGGTCAAAAAGGGAAGAAATCACTAGCAGATAAGTTCGAGTATATCATGCATGGGAAGCTGTACAAACTCGCAGATGAAGGCTCAGGATCAGACTTGAAAGTAGAGGTTTATGCATCTTTTGGAGGACTTCAAATGATGTTGAAGGGAGATCCCCTCCACTGTGCCAAATTCAGGGTAGACCAGAACATGTTCTTGCTCCTCCGAAAGCTCACATGA

Coding sequence (CDS)

ATGGCGGAGCTTCTATTTGATGATATTTTCAAGGTCAAGTGCGTCAACCCAGATGGTAAAAAGTATGATAAAGTTTCTAGAATTGAAGCACGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGTGACAAGATTCTGATGGTCCTGTCTCCTACGCTGAATTATGATGGAGCTCCTGTTACCGGCTATAGTGGTCAAAAAGGGAAGAAATCACTAGCAGATAAGTTCGAGTATATCATGCATGGGAAGCTGTACAAACTCGCAGATGAAGGCTCAGGATCAGACTTGAAAGTAGAGGTTTATGCATCTTTTGGAGGACTTCAAATGATGTTGAAGGGAGATCCCCTCCACTGTGCCAAATTCAGGGTAGACCAGAACATGTTCTTGCTCCTCCGAAAGCTCACATGA

Protein sequence

MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVLSPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQMMLKGDPLHCAKFRVDQNMFLLLRKLT
Homology
BLAST of Csor.00g023910 vs. ExPASy Swiss-Prot
Match: Q9M1A8 (DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=3702 GN=NRPB8B PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.1e-43
Identity = 83/142 (58.45%), Postives = 108/142 (76.06%), Query Frame = 0

Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVLSPT 63
           ++F+DIF V  ++PDGKK+DKV+R+EARS  L M+M LDVNTE+YP+  GDK  + ++PT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P TGY     KK+LADK+EYIMHGKLYK++ E  G   K E+Y SFGGL M+L+
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLQ 125

Query: 124 GDPLHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

BLAST of Csor.00g023910 vs. ExPASy Swiss-Prot
Match: O81097 (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702 GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.4e-43
Identity = 84/142 (59.15%), Postives = 107/142 (75.35%), Query Frame = 0

Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVLSPT 63
           +LF+DIF V  ++PDGKK+DKV+R++A S  L M+M LDVNTE+YP+  GDK  + L+PT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P TGY     KK+LADK+EYIMHGKLYK++ E  G   K E+Y SFGGL M+LK
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLK 125

Query: 124 GDPLHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

BLAST of Csor.00g023910 vs. ExPASy Swiss-Prot
Match: Q19826 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis elegans OX=6239 GN=rpb-8 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 1.4e-32
Identity = 65/145 (44.83%), Postives = 101/145 (69.66%), Query Frame = 0

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MA ++FDD+FKVK V+PDGKK+D+VSR    +E   M +++D+N++IYP+ + DK+ +VL
Sbjct: 1   MAGIIFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG    G    K +     ++EY+M+GK+Y+L D+ +G+D  K+  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDTGTDGGKLAAYASFGGLL 120

Query: 121 MMLKGDPLHCAKFRVDQNMFLLLRK 145
           M LKG+ ++   F VD N++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

BLAST of Csor.00g023910 vs. ExPASy Swiss-Prot
Match: A8XT81 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis briggsae OX=6238 GN=rpb-8 PE=3 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 1.6e-31
Identity = 64/145 (44.14%), Postives = 99/145 (68.28%), Query Frame = 0

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MA ++FDD+F VK V+PDGKK+D+VSR    +E   M +++D+N++IYP+ + DK  +VL
Sbjct: 1   MAGIIFDDMFSVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKFRLVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG    G    K +     ++EY+M+GK+Y+L D+ SG+D  ++  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDSGTDGGQLAAYASFGGLL 120

Query: 121 MMLKGDPLHCAKFRVDQNMFLLLRK 145
           M LKG+ ++   F VD N++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

BLAST of Csor.00g023910 vs. ExPASy Swiss-Prot
Match: P52434 (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Homo sapiens OX=9606 GN=POLR2H PE=1 SV=4)

HSP 1 Score: 132.1 bits (331), Expect = 5.1e-30
Identity = 64/148 (43.24%), Postives = 100/148 (67.57%), Query Frame = 0

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MA +LF+DIF VK ++P+GKK+D+VSR+   SE   M ++LDVN +IYP+D GDK  +V+
Sbjct: 1   MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVI 60

Query: 61  SPTLNYDGAPVTG-YSGQKGKKSLADKFEYIMHGKLYKLADEGSGSD--LKVEVYASFGG 120
           + TL  DG    G Y+    + S AD+FEY+M+GK+Y++  + + ++   ++  Y S+GG
Sbjct: 61  ASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGG 120

Query: 121 LQMMLKGDPLHCAKFRVDQNMFLLLRKL 146
           L M L+GD  +   F VD  ++LL++KL
Sbjct: 121 LLMRLQGDANNLHGFEVDSRVYLLMKKL 148

BLAST of Csor.00g023910 vs. NCBI nr
Match: KAG6600626.1 (DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031262.1 DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 296 bits (758), Expect = 3.20e-101
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146
           MLKGDPLHCAKFRVDQNMFLLLRKLT
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146

BLAST of Csor.00g023910 vs. NCBI nr
Match: XP_022983196.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983203.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983212.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983221.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983229.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima])

HSP 1 Score: 291 bits (745), Expect = 3.08e-99
Identity = 143/146 (97.95%), Postives = 145/146 (99.32%), Query Frame = 0

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MAE+LFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL
Sbjct: 1   MAEVLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVT YSGQ+GKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146
           MLKGDPLHCAKFRVDQNMFLLLRKLT
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146

BLAST of Csor.00g023910 vs. NCBI nr
Match: XP_022942165.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata])

HSP 1 Score: 285 bits (730), Expect = 5.97e-97
Identity = 141/145 (97.24%), Postives = 142/145 (97.93%), Query Frame = 0

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVT YSGQ+GKKSLADKFEYIMH KLYKLADEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHRKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 145
           MLKGDPLHC KFRVDQNMFLLLRKL
Sbjct: 121 MLKGDPLHCVKFRVDQNMFLLLRKL 145

BLAST of Csor.00g023910 vs. NCBI nr
Match: XP_022929200.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022929202.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022985172.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_023551974.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita pepo subsp. pepo] >KAG6577274.1 DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015358.1 DNA-directed RNA polymerases II, IV and V subunit 8B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 282 bits (722), Expect = 9.58e-96
Identity = 136/145 (93.79%), Postives = 144/145 (99.31%), Query Frame = 0

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MAELLF+D+FKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVT YSGQ+GKKSLADKFEYIMHGK+YKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 145
           +LKGDP+HCAKFRVDQNMFLLLRKL
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

BLAST of Csor.00g023910 vs. NCBI nr
Match: XP_038901286.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Benincasa hispida])

HSP 1 Score: 279 bits (713), Expect = 2.42e-94
Identity = 134/144 (93.06%), Postives = 142/144 (98.61%), Query Frame = 0

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MAELLFDDIF VKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MAELLFDDIFMVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVT Y+GQ+GKKSLADKFEYIMHGK+YKL+D+GSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYTGQEGKKSLADKFEYIMHGKIYKLSDDGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRK 144
           +LKGDP+HCAKFRVDQNMFLLLRK
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRK 144

BLAST of Csor.00g023910 vs. ExPASy TrEMBL
Match: A0A6J1J6Q9 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX=3661 GN=LOC111481850 PE=3 SV=1)

HSP 1 Score: 291 bits (745), Expect = 1.49e-99
Identity = 143/146 (97.95%), Postives = 145/146 (99.32%), Query Frame = 0

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MAE+LFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL
Sbjct: 1   MAEVLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVT YSGQ+GKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146
           MLKGDPLHCAKFRVDQNMFLLLRKLT
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146

BLAST of Csor.00g023910 vs. ExPASy TrEMBL
Match: A0A6J1FVR2 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata OX=3662 GN=LOC111447307 PE=3 SV=1)

HSP 1 Score: 285 bits (730), Expect = 2.89e-97
Identity = 141/145 (97.24%), Postives = 142/145 (97.93%), Query Frame = 0

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVT YSGQ+GKKSLADKFEYIMH KLYKLADEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHRKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 145
           MLKGDPLHC KFRVDQNMFLLLRKL
Sbjct: 121 MLKGDPLHCVKFRVDQNMFLLLRKL 145

BLAST of Csor.00g023910 vs. ExPASy TrEMBL
Match: A0A6J1ETP8 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata OX=3662 GN=LOC111435865 PE=3 SV=1)

HSP 1 Score: 282 bits (722), Expect = 4.64e-96
Identity = 136/145 (93.79%), Postives = 144/145 (99.31%), Query Frame = 0

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MAELLF+D+FKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVT YSGQ+GKKSLADKFEYIMHGK+YKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 145
           +LKGDP+HCAKFRVDQNMFLLLRKL
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

BLAST of Csor.00g023910 vs. ExPASy TrEMBL
Match: A0A6J1J454 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX=3661 GN=LOC111483257 PE=3 SV=1)

HSP 1 Score: 282 bits (722), Expect = 4.64e-96
Identity = 136/145 (93.79%), Postives = 144/145 (99.31%), Query Frame = 0

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MAELLF+D+FKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVT YSGQ+GKKSLADKFEYIMHGK+YKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 145
           +LKGDP+HCAKFRVDQNMFLLLRKL
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

BLAST of Csor.00g023910 vs. ExPASy TrEMBL
Match: A0A0A0KZC2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G031020 PE=3 SV=1)

HSP 1 Score: 273 bits (698), Expect = 2.19e-92
Identity = 132/146 (90.41%), Postives = 141/146 (96.58%), Query Frame = 0

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MA LLF+DIFKVKCVNPDGKKYDKVSRIEARSEKLSM+MLLDVNTEIYPIDEG+K+LMVL
Sbjct: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDG+PVT Y  Q+GKKSLADKFEYIMHGK YKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146
           +LKGDP+HCAKFRVDQNMFLLLRKLT
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKLT 146

BLAST of Csor.00g023910 vs. TAIR 10
Match: AT3G59600.1 (RNA polymerase Rpb8 )

HSP 1 Score: 177.6 bits (449), Expect = 7.5e-45
Identity = 83/142 (58.45%), Postives = 108/142 (76.06%), Query Frame = 0

Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVLSPT 63
           ++F+DIF V  ++PDGKK+DKV+R+EARS  L M+M LDVNTE+YP+  GDK  + ++PT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P TGY     KK+LADK+EYIMHGKLYK++ E  G   K E+Y SFGGL M+L+
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLQ 125

Query: 124 GDPLHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

BLAST of Csor.00g023910 vs. TAIR 10
Match: AT1G54250.1 (RNA polymerase Rpb8 )

HSP 1 Score: 177.2 bits (448), Expect = 9.8e-45
Identity = 84/142 (59.15%), Postives = 107/142 (75.35%), Query Frame = 0

Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVLSPT 63
           +LF+DIF V  ++PDGKK+DKV+R++A S  L M+M LDVNTE+YP+  GDK  + L+PT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P TGY     KK+LADK+EYIMHGKLYK++ E  G   K E+Y SFGGL M+LK
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLK 125

Query: 124 GDPLHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M1A81.1e-4358.45DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=... [more]
O810971.4e-4359.15DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702... [more]
Q198261.4e-3244.83Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab... [more]
A8XT811.6e-3144.14Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab... [more]
P524345.1e-3043.24DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Homo sapiens OX=96... [more]
Match NameE-valueIdentityDescription
KAG6600626.13.20e-101100.00DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyros... [more]
XP_022983196.13.08e-9997.95DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP... [more]
XP_022942165.15.97e-9797.24DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata][more]
XP_022929200.19.58e-9693.79DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >... [more]
XP_038901286.12.42e-9493.06DNA-directed RNA polymerases II, IV and V subunit 8B-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1J6Q91.49e-9997.95DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX... [more]
A0A6J1FVR22.89e-9797.24DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata ... [more]
A0A6J1ETP84.64e-9693.79DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata ... [more]
A0A6J1J4544.64e-9693.79DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX... [more]
A0A0A0KZC22.19e-9290.41Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G031020 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G59600.17.5e-4558.45RNA polymerase Rpb8 [more]
AT1G54250.19.8e-4559.15RNA polymerase Rpb8 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005570RNA polymerase, Rpb8SMARTSM00658rpol8neucoord: 2..144
e-value: 5.0E-59
score: 212.1
IPR005570RNA polymerase, Rpb8PFAMPF03870RNA_pol_Rpb8coord: 7..144
e-value: 4.1E-50
score: 169.6
IPR005570RNA polymerase, Rpb8PIRSFPIRSF000779RPB8coord: 1..146
e-value: 1.5E-48
score: 161.8
IPR005570RNA polymerase, Rpb8PANTHERPTHR10917DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3coord: 1..145
NoneNo IPR availableGENE3D2.40.50.140coord: 6..146
e-value: 1.7E-54
score: 185.7
NoneNo IPR availablePANTHERPTHR10917:SF1DNA-DIRECTED RNA POLYMERASE I, IIcoord: 1..145
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 3..144

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g023910.m01Csor.00g023910.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005736 RNA polymerase I complex
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity