Csor.00g021830 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g021830
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionaspartic proteinase CDR1-like
LocationCsor_Chr04: 3026421 .. 3026789 (-)
RNA-Seq ExpressionCsor.00g021830
SyntenyCsor.00g021830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglepolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAAATAAGCTTCAGCAAATAGGCCGTAGTTTTAGGGCGAAAAGTCGTTTCTATACCCATCTCGTGTTAAAAGAACCCGATACCTTCTATTACCTAACTCTGGAAGCAATGTCCGTTGCAAACAAGCGGTTCAAGGCCACGAACGACATGTCGGCCGCCGCAGAACAAGGGAATATCCTTATCGATTCCAGTACAACATTGACAATTTTACCCCCGAATTTCTACAAAGGTGTCGCTTCAACATTGGCGCGTGTTGTTAAAGCGAAGCGAGTGGATGATCCCATAATGGTTGGAAGAACGGTTTGTCTAAACCAATTGACTGATTTAGTTCGACCTATCCAACCCGACTCGAGTGGATTAGACTAA

mRNA sequence

ATGCAAAATAAGCTTCAGCAAATAGGCCGTAGTTTTAGGGCGAAAAGTCGTTTCTATACCCATCTCGTGTTAAAAGAACCCGATACCTTCTATTACCTAACTCTGGAAGCAATGTCCGTTGCAAACAAGCGGTTCAAGGCCACGAACGACATGTCGGCCGCCGCAGAACAAGGGAATATCCTTATCGATTCCAGTACAACATTGACAATTTTACCCCCGAATTTCTACAAAGGTGTCGCTTCAACATTGGCGCGTGTTGTTAAAGCGAAGCGAGTGGATGATCCCATAATGGTTGGAAGAACGGTTTGTCTAAACCAATTGACTGATTTAGTTCGACCTATCCAACCCGACTCGAGTGGATTAGACTAA

Coding sequence (CDS)

ATGCAAAATAAGCTTCAGCAAATAGGCCGTAGTTTTAGGGCGAAAAGTCGTTTCTATACCCATCTCGTGTTAAAAGAACCCGATACCTTCTATTACCTAACTCTGGAAGCAATGTCCGTTGCAAACAAGCGGTTCAAGGCCACGAACGACATGTCGGCCGCCGCAGAACAAGGGAATATCCTTATCGATTCCAGTACAACATTGACAATTTTACCCCCGAATTTCTACAAAGGTGTCGCTTCAACATTGGCGCGTGTTGTTAAAGCGAAGCGAGTGGATGATCCCATAATGGTTGGAAGAACGGTTTGTCTAAACCAATTGACTGATTTAGTTCGACCTATCCAACCCGACTCGAGTGGATTAGACTAA

Protein sequence

MQNKLQQIGRSFRAKSRFYTHLVLKEPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNILIDSSTTLTILPPNFYKGVASTLARVVKAKRVDDPIMVGRTVCLNQLTDLVRPIQPDSSGLD
Homology
BLAST of Csor.00g021830 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.2e-10
Identity = 38/96 (39.58%), Postives = 60/96 (62.50%), Query Frame = 0

Query: 20  THLVLK-EPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNILIDSSTTLTILPPNFYKG 79
           T L+ K   +TFYYLTL+++SV +K+ + +   S ++E GNI+IDS TTLT+LP  FY  
Sbjct: 276 TPLIAKASQETFYYLTLKSISVGSKQIQYSGSDSESSE-GNIIIDSGTTLTLLPTEFYSE 335

Query: 80  VASTLARVVKAKRVDDPIMVGRTVCLNQLTDLVRPI 115
           +   +A  + A++  DP   G ++C +   DL  P+
Sbjct: 336 LEDAVASSIDAEKKQDP-QSGLSLCYSATGDLKVPV 369

BLAST of Csor.00g021830 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 3.2e-09
Identity = 41/84 (48.81%), Postives = 50/84 (59.52%), Query Frame = 0

Query: 20  THLVLKEPDTFYYLTLEAMSVANKRFKAT------NDMSAAAE-QGNILIDSSTTLTILP 79
           T LV KEP T+YYLTLEA+SV  K+   T      ND    +E  GNI+IDS TTLT+L 
Sbjct: 276 TPLVDKEPLTYYYLTLEAISVGKKKIPYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLE 335

Query: 80  PNFYKGVASTLAR-VVKAKRVDDP 96
             F+   +S +   V  AKRV DP
Sbjct: 336 AGFFDKFSSAVEESVTGAKRVSDP 359

BLAST of Csor.00g021830 vs. NCBI nr
Match: KAG6600418.1 (putative aspartic protease, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 242 bits (617), Expect = 1.71e-80
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0

Query: 1   MQNKLQQIGRSFRAKSRFYTHLVLKEPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNI 60
           MQNKLQQIGRSFRAKSRFYTHLVLKEPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNI
Sbjct: 1   MQNKLQQIGRSFRAKSRFYTHLVLKEPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNI 60

Query: 61  LIDSSTTLTILPPNFYKGVASTLARVVKAKRVDDPIMVGRTVCLNQLTDLVRPIQPDSSG 120
           LIDSSTTLTILPPNFYKGVASTLARVVKAKRVDDPIMVGRTVCLNQLTDLVRPIQPDSSG
Sbjct: 61  LIDSSTTLTILPPNFYKGVASTLARVVKAKRVDDPIMVGRTVCLNQLTDLVRPIQPDSSG 120

Query: 121 LD 122
           LD
Sbjct: 121 LD 122

BLAST of Csor.00g021830 vs. NCBI nr
Match: KAG6600420.1 (putative aspartic protease, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 130 bits (327), Expect = 3.72e-33
Identity = 67/76 (88.16%), Postives = 70/76 (92.11%), Query Frame = 0

Query: 20  THLVLKEPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNILIDSSTTLTILPPNFYKGV 79
           T LVLKEP+TFYYLTLEAMSVANKRFKA N+MSAA EQGNILIDS TTLTILP N YKGV
Sbjct: 275 TPLVLKEPNTFYYLTLEAMSVANKRFKAANNMSAAVEQGNILIDSGTTLTILPQNLYKGV 334

Query: 80  ASTLARVVKAKRVDDP 95
           ASTLARVVKAKRV+DP
Sbjct: 335 ASTLARVVKAKRVNDP 350

BLAST of Csor.00g021830 vs. NCBI nr
Match: XP_023543528.1 (aspartic proteinase CDR1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 127 bits (319), Expect = 5.36e-32
Identity = 64/76 (84.21%), Postives = 70/76 (92.11%), Query Frame = 0

Query: 20  THLVLKEPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNILIDSSTTLTILPPNFYKGV 79
           T LVLKEP+TFYY+TL+AMSVANKRFKA N+MSAA E+GNILIDS TTLTILPPN YKGV
Sbjct: 275 TPLVLKEPNTFYYVTLKAMSVANKRFKAANNMSAAVERGNILIDSGTTLTILPPNLYKGV 334

Query: 80  ASTLARVVKAKRVDDP 95
           ASTLA VVKAKRV+DP
Sbjct: 335 ASTLAHVVKAKRVNDP 350

BLAST of Csor.00g021830 vs. NCBI nr
Match: XP_022942027.1 (aspartic proteinase CDR1-like [Cucurbita moschata])

HSP 1 Score: 127 bits (318), Expect = 7.48e-32
Identity = 65/76 (85.53%), Postives = 68/76 (89.47%), Query Frame = 0

Query: 20  THLVLKEPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNILIDSSTTLTILPPNFYKGV 79
           T LVLKEP+TFYYLTLEAMSVANKRFKA N+MS A EQGNILIDS TTLTILP N YKGV
Sbjct: 275 TPLVLKEPNTFYYLTLEAMSVANKRFKAANNMSTAVEQGNILIDSGTTLTILPQNLYKGV 334

Query: 80  ASTLARVVKAKRVDDP 95
           ASTLA VVKAKRV+DP
Sbjct: 335 ASTLAHVVKAKRVNDP 350

BLAST of Csor.00g021830 vs. NCBI nr
Match: XP_022942024.1 (aspartic proteinase CDR1-like [Cucurbita moschata])

HSP 1 Score: 116 bits (290), Expect = 1.32e-29
Identity = 65/93 (69.89%), Postives = 70/93 (75.27%), Query Frame = 0

Query: 2   QNKLQQIGRSFRAKSRFYTHLVLKEPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNIL 61
           Q+KL+Q G  FRAKS FY   V K     +YLTLEAMSVANKRFKA NDMS AAEQGNIL
Sbjct: 76  QSKLRQKGHCFRAKSHFYLSHV-KRTRYLHYLTLEAMSVANKRFKAANDMSVAAEQGNIL 135

Query: 62  IDSSTTLTILPPNFYKGVASTLARVVKAKRVDD 94
           ++  TTLTILP N YK VA TLARVVK KRV D
Sbjct: 136 MNFGTTLTILPSNLYKRVALTLARVVKVKRVHD 167

BLAST of Csor.00g021830 vs. ExPASy TrEMBL
Match: A0A6J1FP39 (aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111447216 PE=3 SV=1)

HSP 1 Score: 127 bits (318), Expect = 3.62e-32
Identity = 65/76 (85.53%), Postives = 68/76 (89.47%), Query Frame = 0

Query: 20  THLVLKEPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNILIDSSTTLTILPPNFYKGV 79
           T LVLKEP+TFYYLTLEAMSVANKRFKA N+MS A EQGNILIDS TTLTILP N YKGV
Sbjct: 275 TPLVLKEPNTFYYLTLEAMSVANKRFKAANNMSTAVEQGNILIDSGTTLTILPQNLYKGV 334

Query: 80  ASTLARVVKAKRVDDP 95
           ASTLA VVKAKRV+DP
Sbjct: 335 ASTLAHVVKAKRVNDP 350

BLAST of Csor.00g021830 vs. ExPASy TrEMBL
Match: A0A6J1FQ48 (aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111447212 PE=4 SV=1)

HSP 1 Score: 116 bits (290), Expect = 6.39e-30
Identity = 65/93 (69.89%), Postives = 70/93 (75.27%), Query Frame = 0

Query: 2   QNKLQQIGRSFRAKSRFYTHLVLKEPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNIL 61
           Q+KL+Q G  FRAKS FY   V K     +YLTLEAMSVANKRFKA NDMS AAEQGNIL
Sbjct: 76  QSKLRQKGHCFRAKSHFYLSHV-KRTRYLHYLTLEAMSVANKRFKAANDMSVAAEQGNIL 135

Query: 62  IDSSTTLTILPPNFYKGVASTLARVVKAKRVDD 94
           ++  TTLTILP N YK VA TLARVVK KRV D
Sbjct: 136 MNFGTTLTILPSNLYKRVALTLARVVKVKRVHD 167

BLAST of Csor.00g021830 vs. ExPASy TrEMBL
Match: A0A5A7TPZ5 (Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G002740 PE=3 SV=1)

HSP 1 Score: 113 bits (283), Expect = 3.77e-27
Identity = 61/96 (63.54%), Postives = 70/96 (72.92%), Query Frame = 0

Query: 20  THLVLKEPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNILIDSSTTLTILPPNFYKGV 79
           T LV + PDTFY+LTLEA+SV NKRFKA  DMSA   QGNI+IDS TTLT+LP + Y GV
Sbjct: 272 TPLVPRSPDTFYFLTLEAISVGNKRFKAAKDMSAMTNQGNIIIDSGTTLTLLPRSLYDGV 331

Query: 80  ASTLARVVKAKRVDDPI-MVGRTVCLNQLTDLVRPI 114
            STLARV+KAKRVDDP  ++       QL DL  PI
Sbjct: 332 VSTLARVIKAKRVDDPSGILELCYSAGQLEDLNIPI 367

BLAST of Csor.00g021830 vs. ExPASy TrEMBL
Match: A0A5D3D1Z7 (Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003070 PE=3 SV=1)

HSP 1 Score: 112 bits (279), Expect = 1.40e-26
Identity = 60/96 (62.50%), Postives = 69/96 (71.88%), Query Frame = 0

Query: 20  THLVLKEPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNILIDSSTTLTILPPNFYKGV 79
           T LV + PDTFY+LTLEA+SV NKRFKA  DMSA   QGNI+IDS TTLT+LP + Y GV
Sbjct: 272 TPLVPRSPDTFYFLTLEAISVGNKRFKAAKDMSAMTNQGNIIIDSGTTLTLLPRSLYDGV 331

Query: 80  ASTLARVVKAKRVDDPI-MVGRTVCLNQLTDLVRPI 114
            STLARV+K KRVDDP  ++       QL DL  PI
Sbjct: 332 VSTLARVIKTKRVDDPSGILELCYSAGQLEDLNIPI 367

BLAST of Csor.00g021830 vs. ExPASy TrEMBL
Match: A0A1S3BT75 (probable aspartic protease At2g35615 OS=Cucumis melo OX=3656 GN=LOC103493255 PE=3 SV=1)

HSP 1 Score: 112 bits (279), Expect = 1.40e-26
Identity = 60/96 (62.50%), Postives = 69/96 (71.88%), Query Frame = 0

Query: 20  THLVLKEPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNILIDSSTTLTILPPNFYKGV 79
           T LV + PDTFY+LTLEA+SV NKRFKA  DMSA   QGNI+IDS TTLT+LP + Y GV
Sbjct: 272 TPLVPRSPDTFYFLTLEAISVGNKRFKAAKDMSAMTNQGNIIIDSGTTLTLLPRSLYDGV 331

Query: 80  ASTLARVVKAKRVDDPI-MVGRTVCLNQLTDLVRPI 114
            STLARV+K KRVDDP  ++       QL DL  PI
Sbjct: 332 VSTLARVIKTKRVDDPSGILELCYSAGQLEDLNIPI 367

BLAST of Csor.00g021830 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 76.3 bits (186), Expect = 2.0e-14
Identity = 38/76 (50.00%), Postives = 54/76 (71.05%), Query Frame = 0

Query: 20  THLVLKEPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNILIDSSTTLTILPPNFYKGV 79
           T +V K+P T+Y+L LEA+SV +K+ + T+ +    E GNI+IDS TTLT+LP NFY  +
Sbjct: 271 TSMVKKDPATYYFLNLEAISVGSKKIQFTSTIFGTGE-GNIVIDSGTTLTLLPSNFYYEL 330

Query: 80  ASTLARVVKAKRVDDP 96
            S +A  +KA+RV DP
Sbjct: 331 ESVVASTIKAERVQDP 345

BLAST of Csor.00g021830 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 66.6 bits (161), Expect = 1.6e-11
Identity = 38/96 (39.58%), Postives = 60/96 (62.50%), Query Frame = 0

Query: 20  THLVLK-EPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNILIDSSTTLTILPPNFYKG 79
           T L+ K   +TFYYLTL+++SV +K+ + +   S ++E GNI+IDS TTLT+LP  FY  
Sbjct: 276 TPLIAKASQETFYYLTLKSISVGSKQIQYSGSDSESSE-GNIIIDSGTTLTLLPTEFYSE 335

Query: 80  VASTLARVVKAKRVDDPIMVGRTVCLNQLTDLVRPI 115
           +   +A  + A++  DP   G ++C +   DL  P+
Sbjct: 336 LEDAVASSIDAEKKQDP-QSGLSLCYSATGDLKVPV 369

BLAST of Csor.00g021830 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 62.8 bits (151), Expect = 2.2e-10
Identity = 41/84 (48.81%), Postives = 50/84 (59.52%), Query Frame = 0

Query: 20  THLVLKEPDTFYYLTLEAMSVANKRFKAT------NDMSAAAE-QGNILIDSSTTLTILP 79
           T LV KEP T+YYLTLEA+SV  K+   T      ND    +E  GNI+IDS TTLT+L 
Sbjct: 276 TPLVDKEPLTYYYLTLEAISVGKKKIPYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLE 335

Query: 80  PNFYKGVASTLAR-VVKAKRVDDP 96
             F+   +S +   V  AKRV DP
Sbjct: 336 AGFFDKFSSAVEESVTGAKRVSDP 359

BLAST of Csor.00g021830 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 58.9 bits (141), Expect = 3.2e-09
Identity = 33/82 (40.24%), Postives = 48/82 (58.54%), Query Frame = 0

Query: 20  THLVLKEPDTFYYLTLEAMSVANKRFKATN-----DMSAAAEQGNILIDSSTTLTILPPN 79
           T L+ K+P+T+Y+LTLEA++V   +   T      +  ++   GNI+IDS TTLT+L   
Sbjct: 276 TPLIQKDPETYYFLTLEAVTVGKTKLPYTGGGYGLNGKSSKRTGNIIIDSGTTLTLLDSG 335

Query: 80  FYKGVASTLAR-VVKAKRVDDP 96
           FY    + +   V  AKRV DP
Sbjct: 336 FYDDFGTAVEESVTGAKRVSDP 357

BLAST of Csor.00g021830 vs. TAIR 10
Match: AT2G28220.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 53.5 bits (127), Expect = 1.4e-07
Identity = 30/74 (40.54%), Postives = 45/74 (60.81%), Query Frame = 0

Query: 22  LVLKEPDTFYYLTLEAMSVANKRFKATNDMSAAAEQGNILIDSSTTLTILPPNFYKGVAS 81
           + +K+ + FYYL L+A+SV + R + T      AE GNI+IDS +T+T  P ++   V  
Sbjct: 253 MFIKKDNPFYYLNLDAVSVEDNRIE-TLGTPFHAEDGNIVIDSGSTVTYFPVSYCNLVRK 312

Query: 82  TLARVVKAKRVDDP 96
            + +VV A RV DP
Sbjct: 313 AVEQVVTAVRVPDP 325

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6XBF82.2e-1039.58Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1[more]
Q3EBM53.2e-0948.81Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561... [more]
Match NameE-valueIdentityDescription
KAG6600418.11.71e-80100.00putative aspartic protease, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6600420.13.72e-3388.16putative aspartic protease, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023543528.15.36e-3284.21aspartic proteinase CDR1-like [Cucurbita pepo subsp. pepo][more]
XP_022942027.17.48e-3285.53aspartic proteinase CDR1-like [Cucurbita moschata][more]
XP_022942024.11.32e-2969.89aspartic proteinase CDR1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1FP393.62e-3285.53aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111447216 PE=3... [more]
A0A6J1FQ486.39e-3069.89aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111447212 PE=4... [more]
A0A5A7TPZ53.77e-2763.54Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A5D3D1Z71.40e-2662.50Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BT751.40e-2662.50probable aspartic protease At2g35615 OS=Cucumis melo OX=3656 GN=LOC103493255 PE=... [more]
Match NameE-valueIdentityDescription
AT1G64830.12.0e-1450.00Eukaryotic aspartyl protease family protein [more]
AT5G33340.11.6e-1139.58Eukaryotic aspartyl protease family protein [more]
AT2G35615.12.2e-1048.81Eukaryotic aspartyl protease family protein [more]
AT1G31450.13.2e-0940.24Eukaryotic aspartyl protease family protein [more]
AT2G28220.11.4e-0740.54Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 12..108
e-value: 6.3E-15
score: 57.3
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 15..103
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 30..94
e-value: 4.2E-8
score: 33.1
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 19..107
NoneNo IPR availablePANTHERPTHR47967:SF66ASPARTIC PROTEINASE CDR1-RELATEDcoord: 19..107

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g021830.m01Csor.00g021830.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005576 extracellular region
molecular_function GO:0004190 aspartic-type endopeptidase activity