Csor.00g020840 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g020840
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationCsor_Chr04: 2507953 .. 2509218 (-)
RNA-Seq ExpressionCsor.00g020840
SyntenyCsor.00g020840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglepolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTGGAAGTGTTGTTGTTGAGCCCTTGGATTTGAGCGTGCAGATTCCTTACCATTTCAGGTGCCCGATTTCTCTCGAGCTCATGCGCGATCCGGTTACGGTTAGCACTGGCCAGACCTATGACCGTTCTAGTATTGAGTCATGGGTGGCCACCGGCAACACTACGTGTCCGGTCACTAGGGCTCCGCTTACGGAGTTTACGCTCATCCCTAACCATACGTTGCGACGCCTTATCCAGGAGTGGTGCGTTGCGAATCGGTCTTATGGCGTTGAGCGGATTCCGACACCGAAGCAACCGGCGGATCCGTCTTTGGTTCGGTCTCTGCTTGGCCAAGCTTCGTCGAAATCGAGTGGTTCGAGTTCGAGAATCTCGGCTGTGCGGCGGTTGAAAGGACTTGCTCGTGACTCGGAGAAGAATCGGTCGTTGATTTCTTCTCTGAATGCTCGTGAAATTCTTATTGATGTTGTGTTTTCGAATTCGGATTCGGATTCGGTTTCTCCTGATTTGAGCCGCGAATCGCTCGCGTTGCTTGTGATGTTGCCGCTCACGGAATCTGAGTGCGTTCTCGTTGCGTCTGATCCGCAACGGATTGGTTACCTGTCACATCTTCTTTTTGATTCTTCGATTGAAGTCCGAATCAATGCCGCTGCGTTGATTGAAATCGTGATTGCAGGAACTCGAGCGTCCGAGCTTCGAGGACAAATTTGCGCTATCGACGAACTATTCGAAGGCGTGGTCGAAATTCTCAGAGATCCAACGGCGTATCCGCGAGCGGTGAAGGTCGGAGTTAAGGCCTTGTTCGCTCTCTGTTTAGTGAAACAAACCAGGCACAAGGCGGTTTCCGCCGGCGCCGCCGAGATTCTCATCGACCGATTCCCCGATTTGGAGAAGTACGACGCGGAGAGAGCCCTAGCAACGATCGAACTCATTTGCAGAATTCCAACTGGTTACGACGCGTTTGCCTCTCACGCGCTCACAGTTCCGCTTTTGGTGAAAGTCATTCTGAAGATCTCAGACAGAGCAACGGAATCAGCAGTCGGAGCTCTGGTATCGCTGTGCTCTGCGTCGGAGGAGAACCGACGGGAGGCGGTGGCGGCAGGGATTTTCACACAGCTGTTGTTGCTGGTGCAAAGCGATTGTACGGAGCGAGTTAAACGGAAATCCCAAGTGCTCCTGAAGCTTCTTCGAGATTCATGGCCGCAGGATTCCGTCGGAAACTCAGACGACTATGGTTGCAGTGAAATTGTGATACCGTTTTATTAA

mRNA sequence

ATGCCTGGAAGTGTTGTTGTTGAGCCCTTGGATTTGAGCGTGCAGATTCCTTACCATTTCAGGTGCCCGATTTCTCTCGAGCTCATGCGCGATCCGGTTACGGTTAGCACTGGCCAGACCTATGACCGTTCTAGTATTGAGTCATGGGTGGCCACCGGCAACACTACGTGTCCGGTCACTAGGGCTCCGCTTACGGAGTTTACGCTCATCCCTAACCATACGTTGCGACGCCTTATCCAGGAGTGGTGCGTTGCGAATCGGTCTTATGGCGTTGAGCGGATTCCGACACCGAAGCAACCGGCGGATCCGTCTTTGGTTCGGTCTCTGCTTGGCCAAGCTTCGTCGAAATCGAGTGGTTCGAGTTCGAGAATCTCGGCTGTGCGGCGGTTGAAAGGACTTGCTCGTGACTCGGAGAAGAATCGGTCGTTGATTTCTTCTCTGAATGCTCGTGAAATTCTTATTGATGTTGTGTTTTCGAATTCGGATTCGGATTCGGTTTCTCCTGATTTGAGCCGCGAATCGCTCGCGTTGCTTGTGATGTTGCCGCTCACGGAATCTGAGTGCGTTCTCGTTGCGTCTGATCCGCAACGGATTGGTTACCTGTCACATCTTCTTTTTGATTCTTCGATTGAAGTCCGAATCAATGCCGCTGCGTTGATTGAAATCGTGATTGCAGGAACTCGAGCGTCCGAGCTTCGAGGACAAATTTGCGCTATCGACGAACTATTCGAAGGCGTGGTCGAAATTCTCAGAGATCCAACGGCGTATCCGCGAGCGGTGAAGGTCGGAGTTAAGGCCTTGTTCGCTCTCTGTTTAGTGAAACAAACCAGGCACAAGGCGGTTTCCGCCGGCGCCGCCGAGATTCTCATCGACCGATTCCCCGATTTGGAGAAGTACGACGCGGAGAGAGCCCTAGCAACGATCGAACTCATTTGCAGAATTCCAACTGGTTACGACGCGTTTGCCTCTCACGCGCTCACAGTTCCGCTTTTGGTGAAAGTCATTCTGAAGATCTCAGACAGAGCAACGGAATCAGCAGTCGGAGCTCTGGTATCGCTGTGCTCTGCGTCGGAGGAGAACCGACGGGAGGCGGTGGCGGCAGGGATTTTCACACAGCTGTTGTTGCTGGTGCAAAGCGATTGTACGGAGCGAGTTAAACGGAAATCCCAAGTGCTCCTGAAGCTTCTTCGAGATTCATGGCCGCAGGATTCCGTCGGAAACTCAGACGACTATGGTTGCAGTGAAATTGTGATACCGTTTTATTAA

Coding sequence (CDS)

ATGCCTGGAAGTGTTGTTGTTGAGCCCTTGGATTTGAGCGTGCAGATTCCTTACCATTTCAGGTGCCCGATTTCTCTCGAGCTCATGCGCGATCCGGTTACGGTTAGCACTGGCCAGACCTATGACCGTTCTAGTATTGAGTCATGGGTGGCCACCGGCAACACTACGTGTCCGGTCACTAGGGCTCCGCTTACGGAGTTTACGCTCATCCCTAACCATACGTTGCGACGCCTTATCCAGGAGTGGTGCGTTGCGAATCGGTCTTATGGCGTTGAGCGGATTCCGACACCGAAGCAACCGGCGGATCCGTCTTTGGTTCGGTCTCTGCTTGGCCAAGCTTCGTCGAAATCGAGTGGTTCGAGTTCGAGAATCTCGGCTGTGCGGCGGTTGAAAGGACTTGCTCGTGACTCGGAGAAGAATCGGTCGTTGATTTCTTCTCTGAATGCTCGTGAAATTCTTATTGATGTTGTGTTTTCGAATTCGGATTCGGATTCGGTTTCTCCTGATTTGAGCCGCGAATCGCTCGCGTTGCTTGTGATGTTGCCGCTCACGGAATCTGAGTGCGTTCTCGTTGCGTCTGATCCGCAACGGATTGGTTACCTGTCACATCTTCTTTTTGATTCTTCGATTGAAGTCCGAATCAATGCCGCTGCGTTGATTGAAATCGTGATTGCAGGAACTCGAGCGTCCGAGCTTCGAGGACAAATTTGCGCTATCGACGAACTATTCGAAGGCGTGGTCGAAATTCTCAGAGATCCAACGGCGTATCCGCGAGCGGTGAAGGTCGGAGTTAAGGCCTTGTTCGCTCTCTGTTTAGTGAAACAAACCAGGCACAAGGCGGTTTCCGCCGGCGCCGCCGAGATTCTCATCGACCGATTCCCCGATTTGGAGAAGTACGACGCGGAGAGAGCCCTAGCAACGATCGAACTCATTTGCAGAATTCCAACTGGTTACGACGCGTTTGCCTCTCACGCGCTCACAGTTCCGCTTTTGGTGAAAGTCATTCTGAAGATCTCAGACAGAGCAACGGAATCAGCAGTCGGAGCTCTGGTATCGCTGTGCTCTGCGTCGGAGGAGAACCGACGGGAGGCGGTGGCGGCAGGGATTTTCACACAGCTGTTGTTGCTGGTGCAAAGCGATTGTACGGAGCGAGTTAAACGGAAATCCCAAGTGCTCCTGAAGCTTCTTCGAGATTCATGGCCGCAGGATTCCGTCGGAAACTCAGACGACTATGGTTGCAGTGAAATTGTGATACCGTTTTATTAA

Protein sequence

MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGSSSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAIDELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYDAERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEENRREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPFY
Homology
BLAST of Csor.00g020840 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 1.4e-152
Identity = 278/424 (65.57%), Postives = 346/424 (81.60%), Query Frame = 0

Query: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60
           MPG+  +EPLDL +QIPYHFRCPISL+LM DPVT+STGQTYDR+SI+SW+A GNTTCPVT
Sbjct: 1   MPGN--LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVT 60

Query: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSS-- 120
           R  L++FTLIPNHTLRRLIQEWCVANRS GVERIPTPKQPADP  VRSLL QAS+ +   
Sbjct: 61  RVALSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTH 120

Query: 121 -GSSSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLAL 180
               SR +A+RRL+GLARDSEKNR LI+  NAREIL+ ++F++ ++ S+S +L  ESLAL
Sbjct: 121 VSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELVSESLAL 180

Query: 181 LVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQIC 240
           LV+L +TE+EC  VASDP R+G+++ LLFDSSIE+R+NAAALIE+V+ G ++ +L+  I 
Sbjct: 181 LVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIIS 240

Query: 241 AIDELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRF-PDL 300
             D +FEGV+++L++P +  RA+K+G+KA+FALCLVKQTRH A+SAGA  ILIDR   D 
Sbjct: 241 GSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADF 300

Query: 301 EKYDAERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSA 360
           ++ D ER LAT+EL+CR+P G  AF  HALTVPL+VK IL++SDRATE A GAL++LC+A
Sbjct: 301 DRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTA 360

Query: 361 SEENRREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEI 420
            E  R EA AAG+ TQLLLLVQSDCTER KRK+Q+LLKLLRDSWP DS  +SDD+  SE 
Sbjct: 361 EERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTVHSDDFNRSE- 420

BLAST of Csor.00g020840 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 5.0e-147
Identity = 274/421 (65.08%), Postives = 339/421 (80.52%), Query Frame = 0

Query: 7   VEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLT 66
           +EPLDL +QIPYHFRCPISLELM+DPVTV TGQTYDR+SIESWV+ G NTTCPVTRAPL+
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 67  EFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSS---GSSS 126
           +FTLIPNHTLRRLIQEWCVANRS GVERIPTPKQPADP+ VR+LL QAS+ +       S
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRS 124

Query: 127 RISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVMLP 186
           R +A+RRL+G ARDS+KNR LI++ NA EILI ++F    S++ S +L  ESLALLVMLP
Sbjct: 125 RAAALRRLRGFARDSDKNRVLIAAHNATEILIKILF----SETTSSELVSESLALLVMLP 184

Query: 187 LTE-SECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAIDE 246
           +TE ++ V ++SDP R+ +L+ LLFDSSIE R+NAAALIEIV  GT++++L+G I   + 
Sbjct: 185 ITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSES 244

Query: 247 LFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRF-PDLEKYD 306
           +FEGV+++LR+P +  RA+K+G+K LFALC VK TRH A++AGA EILIDR   D ++ D
Sbjct: 245 VFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCD 304

Query: 307 AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 366
            ERALAT+EL+CR P G  AF  HALTVPLLVK IL++SDRATE A GAL++LC+A E  
Sbjct: 305 TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAEERW 364

Query: 367 RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQ-DSVGNSDDYGCSEIVIP 421
           R EA  AG+  QLLL+VQS+CTER K+K+Q LLKLLRDSWP  +S  NSDD+GCS  V+P
Sbjct: 365 REEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANSDDFGCSSQVVP 421

BLAST of Csor.00g020840 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 6.7e-51
Identity = 132/403 (32.75%), Postives = 216/403 (53.60%), Query Frame = 0

Query: 10  LDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTEFT 69
           +D  ++IP  F CPISLE+M+DPV VSTG TYDR SIE W+  G   +CPVT+  +T+  
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 70  LIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGSSSRISAVR 129
           L PNHTLRRLIQ WC  N SYGVERIPTP+ P   S +  L+      +S   +++  ++
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLI---RDSASSHENQVKCLK 125

Query: 130 RLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVMLPLTESEC 189
           RL+ +  ++  N+  + +    E L ++V ++S++ S++ +      AL ++  L  SE 
Sbjct: 126 RLRQIVSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDE------ALNLLYHLETSET 185

Query: 190 VLV-----ASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAIDELF 249
           VL        D   +  L+ ++     E R+ A  L++ ++     ++    +    E+F
Sbjct: 186 VLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNIL---EVADPMQSMTLKPEVF 245

Query: 250 EGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPD----LEKY 309
             VV+IL D  +  +A K  +  L  +C   + RHKAV AG   ++I+   D     E+ 
Sbjct: 246 TEVVQILDDRIS-QKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERR 305

Query: 310 DAERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSL--CSAS 369
             E A+  ++L+C+   G   F +H   + ++ K IL++S  A++ AV  L+S+    A+
Sbjct: 306 GPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCAT 365

Query: 370 EENRREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 401
                E +  G+  +L L++Q  C  + K K++ LLKL    W
Sbjct: 366 PALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVW 395

BLAST of Csor.00g020840 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 8.2e-49
Identity = 135/426 (31.69%), Postives = 222/426 (52.11%), Query Frame = 0

Query: 10  LDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTEFT 69
           +D  ++IP  F CPISL++M+DPV VSTG TYDR SIE W+ +G   +CPVT+  +TE  
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 70  LIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGSSSRISAVR 129
           L PNHTLRRLIQ WC  N SYG+ERIPTPK P   S +  L+ ++   SS   +++  ++
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKES---SSSHLNQVKCLK 120

Query: 130 RLKGLARDSEKNRSLISSLNAREILIDVVFSNSDS-DSVSPDLSRESL------------ 189
           RL+ +  ++  N+  + +    E L ++V ++ D+ +S S  LS  +L            
Sbjct: 121 RLRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENR 180

Query: 190 ----------ALLVMLPLTESECVLVA-----SDPQRIGYLSHLLFDSSIEVRINAAALI 249
                     AL V+  L  SE  L +          +  L+ ++     E R  AA L+
Sbjct: 181 FDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLL 240

Query: 250 EIVIAGTRASELRGQICAIDELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKA 309
           + ++      ++   I    ELF  V++IL D  ++ +A +  ++ L   C   + RHKA
Sbjct: 241 KKLLEVADPMQI---ILLERELFGEVIQILHDQISH-KATRSAMQILVITCPWGRNRHKA 300

Query: 310 VSAGAAEILIDRFPD----LEKYDAERALATIELICRIPTGYDAFASHALTVPLLVKVIL 369
           V  G   ++I+   D     E+ ++E A+  ++++C+   G   F +H   + ++ K IL
Sbjct: 301 VEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKIL 360

Query: 370 KISDRATESAVGALVSL--CSASEENRREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLK 401
           ++S   +E AV  L+S+    A+    +E +  G+  +L L++Q  C  + K K++ LLK
Sbjct: 361 RVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLK 419

BLAST of Csor.00g020840 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 6.5e-46
Identity = 128/388 (32.99%), Postives = 207/388 (53.35%), Query Frame = 0

Query: 14  VQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRAPLTEFTLIPNH 73
           + IP  F+CPIS++LM+DPV +STG TYDR SIE+W+ +GN TCPVT   LT F  IPNH
Sbjct: 29  ITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPNH 88

Query: 74  TLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKS-SGSSSRISA-VRRLK 133
           T+R++IQ WCV   S  ++RIPTP+ P  P  V  +  + SS +  G   +    + ++K
Sbjct: 89  TIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKIK 148

Query: 134 GLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVMLPLTESECVLV 193
            L  +SEKNR  ++  +   +L D  F     D     +  E L+LL  +     E +  
Sbjct: 149 KLGDESEKNRKCVNENSVGWVLCD-CFDKFSGDEKLTFMLNEILSLLTWMFPIGLEGISK 208

Query: 194 ASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAIDE-LFEGVVEIL 253
            +       ++ LL  +   VR NAA +++ +++     E R    A++  + E +V+++
Sbjct: 209 LASATSFRCVAGLLKSTDDSVRQNAAFIMKEILS---LDETRVHSFAVENGVAEALVKLI 268

Query: 254 RDPTAYPRAVKVGVKALFALCLVK-QTRHKAVSAGAAEILIDRFPDLEKYDAERALATIE 313
           RD  +   + K  + A++ + L K +   + +  G   I ++   D E    E+ALA ++
Sbjct: 269 RDSVS-SSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDAENSVCEKALAVLD 328

Query: 314 LICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEENR-REAVAAG 373
            IC    G +    +AL +PLLVK I K+S+ AT S++  ++ L          +AV  G
Sbjct: 329 AICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVAVEDAVRLG 388

Query: 374 IFTQLLLLVQSDCTERVKRKSQVLLKLL 397
            F ++LL++Q    E  K K+  LLK++
Sbjct: 389 AFQKVLLVLQVGYGEETKEKATELLKMM 411

BLAST of Csor.00g020840 vs. NCBI nr
Match: XP_022942824.1 (U-box domain-containing protein 26-like [Cucurbita moschata] >KAG6600321.1 U-box domain-containing protein 26, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030978.1 U-box domain-containing protein 26, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 802 bits (2072), Expect = 2.56e-292
Identity = 421/421 (100.00%), Postives = 421/421 (100.00%), Query Frame = 0

Query: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60
           MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT
Sbjct: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60

Query: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS 120
           RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS
Sbjct: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS 120

Query: 121 SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVM 180
           SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVM
Sbjct: 121 SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVM 180

Query: 181 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 240
           LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID
Sbjct: 181 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 240

Query: 241 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 300
           ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD
Sbjct: 241 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 300

Query: 301 AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 360
           AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN
Sbjct: 301 AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 360

Query: 361 RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420
           RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF
Sbjct: 361 RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420

BLAST of Csor.00g020840 vs. NCBI nr
Match: XP_022981075.1 (U-box domain-containing protein 26-like [Cucurbita maxima])

HSP 1 Score: 800 bits (2066), Expect = 2.10e-291
Identity = 419/421 (99.52%), Postives = 421/421 (100.00%), Query Frame = 0

Query: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60
           MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT
Sbjct: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60

Query: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS 120
           RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS
Sbjct: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS 120

Query: 121 SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVM 180
           SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVM
Sbjct: 121 SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVM 180

Query: 181 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 240
           LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID
Sbjct: 181 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 240

Query: 241 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 300
           ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD
Sbjct: 241 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 300

Query: 301 AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 360
           AERALATIELICRIPTGYDAFA+HALTVPLLVKVILKISDRATESAVGALVSLCSASEEN
Sbjct: 301 AERALATIELICRIPTGYDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 360

Query: 361 RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420
           RREAVAAGIFTQLLLLVQSDCT+RVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF
Sbjct: 361 RREAVAAGIFTQLLLLVQSDCTDRVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420

BLAST of Csor.00g020840 vs. NCBI nr
Match: XP_023547970.1 (U-box domain-containing protein 26-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 798 bits (2060), Expect = 1.72e-290
Identity = 418/421 (99.29%), Postives = 420/421 (99.76%), Query Frame = 0

Query: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60
           MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT
Sbjct: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60

Query: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS 120
           RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS
Sbjct: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS 120

Query: 121 SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVM 180
           SSRISAVRRLKGLARDSEKNR LISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVM
Sbjct: 121 SSRISAVRRLKGLARDSEKNRLLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVM 180

Query: 181 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 240
           LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID
Sbjct: 181 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 240

Query: 241 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 300
           ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD
Sbjct: 241 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 300

Query: 301 AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 360
           AERALATIELICRIPTGYDAFA+HALTVPLLVKVILKISDRATESAVGALVSLCSASEEN
Sbjct: 301 AERALATIELICRIPTGYDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 360

Query: 361 RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420
           RREAVAAGIFTQLLLLVQSDCT+RVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF
Sbjct: 361 RREAVAAGIFTQLLLLVQSDCTDRVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420

BLAST of Csor.00g020840 vs. NCBI nr
Match: XP_038875257.1 (U-box domain-containing protein 26-like isoform X2 [Benincasa hispida] >XP_038875267.1 U-box domain-containing protein 26-like isoform X3 [Benincasa hispida])

HSP 1 Score: 752 bits (1941), Expect = 2.72e-272
Identity = 394/420 (93.81%), Postives = 407/420 (96.90%), Query Frame = 0

Query: 3   GSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62
           GSV VEPLDLSV IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 5   GSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64

Query: 63  PLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGSSS 122
           PLT+FTLIPNHTLRRLIQ+WCVANRSYGVERIPTPKQPA+PSLVRSLL QASS+SSG + 
Sbjct: 65  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLSQASSRSSGLNL 124

Query: 123 RISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSN--SDSDSVSPDLSRESLALLVM 182
           RISA+RRLKGLARDS+KNRSLISSLNAREIL+DVVFSN  SDSDS SPDLSRESLALLVM
Sbjct: 125 RISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSFSPDLSRESLALLVM 184

Query: 183 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 242
           LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIE VIAGTRASELR QIC ID
Sbjct: 185 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGTRASELRSQICGID 244

Query: 243 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 302
           ELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAGAAEI+IDRFPDLEKYD
Sbjct: 245 ELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDLEKYD 304

Query: 303 AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 362
           AERALATIELICRIPTG DAFA+HALTVPLLVKVILKISDRATESAVGALVSLCSASEEN
Sbjct: 305 AERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 364

Query: 363 RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420
           RREAVAAGI TQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQ+SVGNSDDYGCSEIV+PF
Sbjct: 365 RREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYGCSEIVVPF 424

BLAST of Csor.00g020840 vs. NCBI nr
Match: XP_008456137.1 (PREDICTED: U-box domain-containing protein 26-like [Cucumis melo] >KAA0037126.1 U-box domain-containing protein 26-like [Cucumis melo var. makuwa] >TYK13948.1 U-box domain-containing protein 26-like [Cucumis melo var. makuwa])

HSP 1 Score: 752 bits (1941), Expect = 2.72e-272
Identity = 393/420 (93.57%), Postives = 408/420 (97.14%), Query Frame = 0

Query: 3   GSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62
           GSV V+PLDLS+ IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 5   GSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64

Query: 63  PLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGSSS 122
           PLT+FTLIPNHTLRRLIQ+WCVANRSYGVERIPTPKQPA+PSLVRSLLGQASS+SSGSS 
Sbjct: 65  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSSGSSL 124

Query: 123 RISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDS--DSVSPDLSRESLALLVM 182
           RISA+RRLKGLARDS+KNRSLISSLNAREIL+DVVFSN DS  DS SPDLSRESLALLVM
Sbjct: 125 RISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSGSDSFSPDLSRESLALLVM 184

Query: 183 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 242
           LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIE VIAGTRASELR QIC ID
Sbjct: 185 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGTRASELRTQICGID 244

Query: 243 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 302
           ELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAGAAEI+IDRFPDLEKYD
Sbjct: 245 ELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDLEKYD 304

Query: 303 AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 362
           AERALATIELICR+PTG DAFA+HALTVPLLVKVILKISDRATESAVGALVSLCSASEEN
Sbjct: 305 AERALATIELICRVPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 364

Query: 363 RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420
           RREAVAAGI TQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQ+SVGNSDDYGCSEIV+PF
Sbjct: 365 RREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYGCSEIVVPF 424

BLAST of Csor.00g020840 vs. ExPASy TrEMBL
Match: A0A6J1FSF9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447739 PE=4 SV=1)

HSP 1 Score: 802 bits (2072), Expect = 1.24e-292
Identity = 421/421 (100.00%), Postives = 421/421 (100.00%), Query Frame = 0

Query: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60
           MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT
Sbjct: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60

Query: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS 120
           RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS
Sbjct: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS 120

Query: 121 SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVM 180
           SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVM
Sbjct: 121 SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVM 180

Query: 181 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 240
           LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID
Sbjct: 181 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 240

Query: 241 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 300
           ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD
Sbjct: 241 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 300

Query: 301 AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 360
           AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN
Sbjct: 301 AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 360

Query: 361 RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420
           RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF
Sbjct: 361 RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420

BLAST of Csor.00g020840 vs. ExPASy TrEMBL
Match: A0A6J1IYF7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111480329 PE=4 SV=1)

HSP 1 Score: 800 bits (2066), Expect = 1.02e-291
Identity = 419/421 (99.52%), Postives = 421/421 (100.00%), Query Frame = 0

Query: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60
           MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT
Sbjct: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60

Query: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS 120
           RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS
Sbjct: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGS 120

Query: 121 SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVM 180
           SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVM
Sbjct: 121 SSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVM 180

Query: 181 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 240
           LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID
Sbjct: 181 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 240

Query: 241 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 300
           ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD
Sbjct: 241 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 300

Query: 301 AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 360
           AERALATIELICRIPTGYDAFA+HALTVPLLVKVILKISDRATESAVGALVSLCSASEEN
Sbjct: 301 AERALATIELICRIPTGYDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 360

Query: 361 RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420
           RREAVAAGIFTQLLLLVQSDCT+RVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF
Sbjct: 361 RREAVAAGIFTQLLLLVQSDCTDRVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420

BLAST of Csor.00g020840 vs. ExPASy TrEMBL
Match: A0A5A7T4S1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold832G001500 PE=4 SV=1)

HSP 1 Score: 752 bits (1941), Expect = 1.32e-272
Identity = 393/420 (93.57%), Postives = 408/420 (97.14%), Query Frame = 0

Query: 3   GSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62
           GSV V+PLDLS+ IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 5   GSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64

Query: 63  PLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGSSS 122
           PLT+FTLIPNHTLRRLIQ+WCVANRSYGVERIPTPKQPA+PSLVRSLLGQASS+SSGSS 
Sbjct: 65  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSSGSSL 124

Query: 123 RISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDS--DSVSPDLSRESLALLVM 182
           RISA+RRLKGLARDS+KNRSLISSLNAREIL+DVVFSN DS  DS SPDLSRESLALLVM
Sbjct: 125 RISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSGSDSFSPDLSRESLALLVM 184

Query: 183 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 242
           LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIE VIAGTRASELR QIC ID
Sbjct: 185 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGTRASELRTQICGID 244

Query: 243 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 302
           ELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAGAAEI+IDRFPDLEKYD
Sbjct: 245 ELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDLEKYD 304

Query: 303 AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 362
           AERALATIELICR+PTG DAFA+HALTVPLLVKVILKISDRATESAVGALVSLCSASEEN
Sbjct: 305 AERALATIELICRVPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 364

Query: 363 RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420
           RREAVAAGI TQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQ+SVGNSDDYGCSEIV+PF
Sbjct: 365 RREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYGCSEIVVPF 424

BLAST of Csor.00g020840 vs. ExPASy TrEMBL
Match: A0A1S3C380 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103496167 PE=4 SV=1)

HSP 1 Score: 752 bits (1941), Expect = 1.32e-272
Identity = 393/420 (93.57%), Postives = 408/420 (97.14%), Query Frame = 0

Query: 3   GSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62
           GSV V+PLDLS+ IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 5   GSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64

Query: 63  PLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGSSS 122
           PLT+FTLIPNHTLRRLIQ+WCVANRSYGVERIPTPKQPA+PSLVRSLLGQASS+SSGSS 
Sbjct: 65  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSSGSSL 124

Query: 123 RISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDS--DSVSPDLSRESLALLVM 182
           RISA+RRLKGLARDS+KNRSLISSLNAREIL+DVVFSN DS  DS SPDLSRESLALLVM
Sbjct: 125 RISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSGSDSFSPDLSRESLALLVM 184

Query: 183 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 242
           LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIE VIAGTRASELR QIC ID
Sbjct: 185 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGTRASELRTQICGID 244

Query: 243 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 302
           ELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAGAAEI+IDRFPDLEKYD
Sbjct: 245 ELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDLEKYD 304

Query: 303 AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 362
           AERALATIELICR+PTG DAFA+HALTVPLLVKVILKISDRATESAVGALVSLCSASEEN
Sbjct: 305 AERALATIELICRVPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 364

Query: 363 RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420
           RREAVAAGI TQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQ+SVGNSDDYGCSEIV+PF
Sbjct: 365 RREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYGCSEIVVPF 424

BLAST of Csor.00g020840 vs. ExPASy TrEMBL
Match: A0A0A0L6F8 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G203270 PE=4 SV=1)

HSP 1 Score: 746 bits (1926), Expect = 2.54e-270
Identity = 392/420 (93.33%), Postives = 405/420 (96.43%), Query Frame = 0

Query: 3   GSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62
           GSV V+PLDLS+ IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 5   GSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64

Query: 63  PLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGSSS 122
           PLT+FTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPA+PSLVRSLL QASS+SSGSS 
Sbjct: 65  PLTDFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPAEPSLVRSLLAQASSRSSGSSL 124

Query: 123 RISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDS--DSVSPDLSRESLALLVM 182
           RISA+RRLKGLARDS+KNRSLISSLNAREIL+DVVFSN DS  DS SPDLSRESLALLVM
Sbjct: 125 RISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSGSDSFSPDLSRESLALLVM 184

Query: 183 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 242
           LPLTESECVLVASDPQRIGYLSHLLFDSSIE RINAAALIE VIAGTRASELR QIC ID
Sbjct: 185 LPLTESECVLVASDPQRIGYLSHLLFDSSIEDRINAAALIETVIAGTRASELRTQICGID 244

Query: 243 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 302
           ELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAGAAEI+IDRFPDLEKYD
Sbjct: 245 ELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDLEKYD 304

Query: 303 AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 362
           AERALATIELICRIPTG DAFA+HALTVPLLVKVILKISDRATESAVGALVSLCSASEEN
Sbjct: 305 AERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 364

Query: 363 RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420
           RREAVAAGI TQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQ+SVGNSDDY CSEIV+PF
Sbjct: 365 RREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYACSEIVVPF 424

BLAST of Csor.00g020840 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 540.8 bits (1392), Expect = 9.7e-154
Identity = 278/424 (65.57%), Postives = 346/424 (81.60%), Query Frame = 0

Query: 1   MPGSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVT 60
           MPG+  +EPLDL +QIPYHFRCPISL+LM DPVT+STGQTYDR+SI+SW+A GNTTCPVT
Sbjct: 1   MPGN--LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVT 60

Query: 61  RAPLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSS-- 120
           R  L++FTLIPNHTLRRLIQEWCVANRS GVERIPTPKQPADP  VRSLL QAS+ +   
Sbjct: 61  RVALSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTH 120

Query: 121 -GSSSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLAL 180
               SR +A+RRL+GLARDSEKNR LI+  NAREIL+ ++F++ ++ S+S +L  ESLAL
Sbjct: 121 VSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELVSESLAL 180

Query: 181 LVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQIC 240
           LV+L +TE+EC  VASDP R+G+++ LLFDSSIE+R+NAAALIE+V+ G ++ +L+  I 
Sbjct: 181 LVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIIS 240

Query: 241 AIDELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRF-PDL 300
             D +FEGV+++L++P +  RA+K+G+KA+FALCLVKQTRH A+SAGA  ILIDR   D 
Sbjct: 241 GSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADF 300

Query: 301 EKYDAERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSA 360
           ++ D ER LAT+EL+CR+P G  AF  HALTVPL+VK IL++SDRATE A GAL++LC+A
Sbjct: 301 DRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTA 360

Query: 361 SEENRREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEI 420
            E  R EA AAG+ TQLLLLVQSDCTER KRK+Q+LLKLLRDSWP DS  +SDD+  SE 
Sbjct: 361 EERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTVHSDDFNRSE- 420

BLAST of Csor.00g020840 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 522.3 bits (1344), Expect = 3.6e-148
Identity = 274/421 (65.08%), Postives = 339/421 (80.52%), Query Frame = 0

Query: 7   VEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLT 66
           +EPLDL +QIPYHFRCPISLELM+DPVTV TGQTYDR+SIESWV+ G NTTCPVTRAPL+
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 67  EFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSS---GSSS 126
           +FTLIPNHTLRRLIQEWCVANRS GVERIPTPKQPADP+ VR+LL QAS+ +       S
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRS 124

Query: 127 RISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVMLP 186
           R +A+RRL+G ARDS+KNR LI++ NA EILI ++F    S++ S +L  ESLALLVMLP
Sbjct: 125 RAAALRRLRGFARDSDKNRVLIAAHNATEILIKILF----SETTSSELVSESLALLVMLP 184

Query: 187 LTE-SECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAIDE 246
           +TE ++ V ++SDP R+ +L+ LLFDSSIE R+NAAALIEIV  GT++++L+G I   + 
Sbjct: 185 ITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSES 244

Query: 247 LFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRF-PDLEKYD 306
           +FEGV+++LR+P +  RA+K+G+K LFALC VK TRH A++AGA EILIDR   D ++ D
Sbjct: 245 VFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCD 304

Query: 307 AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 366
            ERALAT+EL+CR P G  AF  HALTVPLLVK IL++SDRATE A GAL++LC+A E  
Sbjct: 305 TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAEERW 364

Query: 367 RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQ-DSVGNSDDYGCSEIVIP 421
           R EA  AG+  QLLL+VQS+CTER K+K+Q LLKLLRDSWP  +S  NSDD+GCS  V+P
Sbjct: 365 REEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANSDDFGCSSQVVP 421

BLAST of Csor.00g020840 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 203.0 bits (515), Expect = 4.8e-52
Identity = 132/403 (32.75%), Postives = 216/403 (53.60%), Query Frame = 0

Query: 10  LDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTEFT 69
           +D  ++IP  F CPISLE+M+DPV VSTG TYDR SIE W+  G   +CPVT+  +T+  
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 70  LIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGSSSRISAVR 129
           L PNHTLRRLIQ WC  N SYGVERIPTP+ P   S +  L+      +S   +++  ++
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLI---RDSASSHENQVKCLK 125

Query: 130 RLKGLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVMLPLTESEC 189
           RL+ +  ++  N+  + +    E L ++V ++S++ S++ +      AL ++  L  SE 
Sbjct: 126 RLRQIVSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDE------ALNLLYHLETSET 185

Query: 190 VLV-----ASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAIDELF 249
           VL        D   +  L+ ++     E R+ A  L++ ++     ++    +    E+F
Sbjct: 186 VLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNIL---EVADPMQSMTLKPEVF 245

Query: 250 EGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPD----LEKY 309
             VV+IL D  +  +A K  +  L  +C   + RHKAV AG   ++I+   D     E+ 
Sbjct: 246 TEVVQILDDRIS-QKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERR 305

Query: 310 DAERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSL--CSAS 369
             E A+  ++L+C+   G   F +H   + ++ K IL++S  A++ AV  L+S+    A+
Sbjct: 306 GPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCAT 365

Query: 370 EENRREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 401
                E +  G+  +L L++Q  C  + K K++ LLKL    W
Sbjct: 366 PALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVW 395

BLAST of Csor.00g020840 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 196.1 bits (497), Expect = 5.9e-50
Identity = 135/426 (31.69%), Postives = 222/426 (52.11%), Query Frame = 0

Query: 10  LDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTEFT 69
           +D  ++IP  F CPISL++M+DPV VSTG TYDR SIE W+ +G   +CPVT+  +TE  
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 70  LIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGSSSRISAVR 129
           L PNHTLRRLIQ WC  N SYG+ERIPTPK P   S +  L+ ++   SS   +++  ++
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKES---SSSHLNQVKCLK 120

Query: 130 RLKGLARDSEKNRSLISSLNAREILIDVVFSNSDS-DSVSPDLSRESL------------ 189
           RL+ +  ++  N+  + +    E L ++V ++ D+ +S S  LS  +L            
Sbjct: 121 RLRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENR 180

Query: 190 ----------ALLVMLPLTESECVLVA-----SDPQRIGYLSHLLFDSSIEVRINAAALI 249
                     AL V+  L  SE  L +          +  L+ ++     E R  AA L+
Sbjct: 181 FDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLL 240

Query: 250 EIVIAGTRASELRGQICAIDELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKA 309
           + ++      ++   I    ELF  V++IL D  ++ +A +  ++ L   C   + RHKA
Sbjct: 241 KKLLEVADPMQI---ILLERELFGEVIQILHDQISH-KATRSAMQILVITCPWGRNRHKA 300

Query: 310 VSAGAAEILIDRFPD----LEKYDAERALATIELICRIPTGYDAFASHALTVPLLVKVIL 369
           V  G   ++I+   D     E+ ++E A+  ++++C+   G   F +H   + ++ K IL
Sbjct: 301 VEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKIL 360

Query: 370 KISDRATESAVGALVSL--CSASEENRREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLK 401
           ++S   +E AV  L+S+    A+    +E +  G+  +L L++Q  C  + K K++ LLK
Sbjct: 361 RVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLK 419

BLAST of Csor.00g020840 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 186.4 bits (472), Expect = 4.6e-47
Identity = 128/388 (32.99%), Postives = 207/388 (53.35%), Query Frame = 0

Query: 14  VQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRAPLTEFTLIPNH 73
           + IP  F+CPIS++LM+DPV +STG TYDR SIE+W+ +GN TCPVT   LT F  IPNH
Sbjct: 29  ITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPNH 88

Query: 74  TLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKS-SGSSSRISA-VRRLK 133
           T+R++IQ WCV   S  ++RIPTP+ P  P  V  +  + SS +  G   +    + ++K
Sbjct: 89  TIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKIK 148

Query: 134 GLARDSEKNRSLISSLNAREILIDVVFSNSDSDSVSPDLSRESLALLVMLPLTESECVLV 193
            L  +SEKNR  ++  +   +L D  F     D     +  E L+LL  +     E +  
Sbjct: 149 KLGDESEKNRKCVNENSVGWVLCD-CFDKFSGDEKLTFMLNEILSLLTWMFPIGLEGISK 208

Query: 194 ASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAIDE-LFEGVVEIL 253
            +       ++ LL  +   VR NAA +++ +++     E R    A++  + E +V+++
Sbjct: 209 LASATSFRCVAGLLKSTDDSVRQNAAFIMKEILS---LDETRVHSFAVENGVAEALVKLI 268

Query: 254 RDPTAYPRAVKVGVKALFALCLVK-QTRHKAVSAGAAEILIDRFPDLEKYDAERALATIE 313
           RD  +   + K  + A++ + L K +   + +  G   I ++   D E    E+ALA ++
Sbjct: 269 RDSVS-SSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDAENSVCEKALAVLD 328

Query: 314 LICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEENR-REAVAAG 373
            IC    G +    +AL +PLLVK I K+S+ AT S++  ++ L          +AV  G
Sbjct: 329 AICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVAVEDAVRLG 388

Query: 374 IFTQLLLLVQSDCTERVKRKSQVLLKLL 397
            F ++LL++Q    E  K K+  LLK++
Sbjct: 389 AFQKVLLVLQVGYGEETKEKATELLKMM 411

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FXA41.4e-15265.57U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Q9LT795.0e-14765.08U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q84TG36.7e-5132.75E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC68.2e-4931.69E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q5PNY66.5e-4632.99U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2... [more]
Match NameE-valueIdentityDescription
XP_022942824.12.56e-292100.00U-box domain-containing protein 26-like [Cucurbita moschata] >KAG6600321.1 U-box... [more]
XP_022981075.12.10e-29199.52U-box domain-containing protein 26-like [Cucurbita maxima][more]
XP_023547970.11.72e-29099.29U-box domain-containing protein 26-like [Cucurbita pepo subsp. pepo][more]
XP_038875257.12.72e-27293.81U-box domain-containing protein 26-like isoform X2 [Benincasa hispida] >XP_03887... [more]
XP_008456137.12.72e-27293.57PREDICTED: U-box domain-containing protein 26-like [Cucumis melo] >KAA0037126.1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FSF91.24e-292100.00RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447739... [more]
A0A6J1IYF71.02e-29199.52RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111480329 P... [more]
A0A5A7T4S11.32e-27293.57RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C3801.32e-27293.57RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103496167 PE=4 ... [more]
A0A0A0L6F82.54e-27093.33RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G203270 PE... [more]
Match NameE-valueIdentityDescription
AT1G49780.19.7e-15465.57plant U-box 26 [more]
AT3G19380.13.6e-14865.08plant U-box 25 [more]
AT2G35930.14.8e-5232.75plant U-box 23 [more]
AT3G52450.15.9e-5031.69plant U-box 22 [more]
AT5G37490.14.6e-4732.99ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 19..82
e-value: 1.4E-34
score: 130.9
IPR003613U box domainPFAMPF04564U-boxcoord: 16..87
e-value: 5.5E-22
score: 77.7
IPR003613U box domainPROSITEPS51698U_BOXcoord: 15..89
score: 44.637169
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 92..407
e-value: 1.3E-18
score: 68.8
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 9..91
e-value: 2.3E-30
score: 106.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 108..123
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 93..123
NoneNo IPR availablePANTHERPTHR22849:SF141RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 7..417
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 7..417
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 20..61
e-value: 2.63047E-25
score: 95.3782
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 13..91
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 106..396

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g020840.m01Csor.00g020840.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity