Csor.00g015030 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g015030
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionaspartic proteinase PCS1-like
LocationCsor_Chr01: 6073951 .. 6075093 (-)
RNA-Seq ExpressionCsor.00g015030
SyntenyCsor.00g015030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglepolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCTCTCTCTCCTATTTCTCTCCCTATTCTGTCTCTTATTCTCCCCATCAAACTCCCTCTCCCTCTCCCTCCCTCTGACCTACCATTCTGTCTCCTCCCAAGAAGCCTCCCTTTCCCTCTCCTCCAAAACCAAATCCCATGGGAAGTTCCCCTTCCAGTACTCCAACGCCCTTGTGGTGTCTGTTCCCATTGGATCGCCGCCGCAGCAGATGGACATGGTCGTAGACACTGGCAGCCAACTGTCCTGGATTCAATGCCACGGCAAAGTAAGGAGAAAATCAGTGAAGCCCATGATTAATTGGTTTGACCCTTATCTCTCCTCCTCTTTCTCTTTCCTTCCCTGTAACACCACTCTGTGCAGACCCCGAATTCCCGATTTTACCCTTCCTACTTCTTGTGACCCAACTCGCCACTGCCACTACTCCTACTTCTACGCGGATGGGACCCTGGCCGAGGGTAATCTCGTCACTGAAAAATTCACCTTCTCCAATTCCTTAACTACCCGCTCCCTCCTTCTGGGCTGTGCTACGGCCTCCACCCAAAACAGGGGTATGTTGGGAATGAATACTGGACGCCTCTCCTTCATCTCCCAGGCCAAAATATCCAAGTTTTCCTATTGCGTTCCTGATCGAACCGGGTCGGATCTAACCGGCTTGTTCTACCTTGGAGACAACCCCAACTCCGCTAAATTCAAATACGTCAACATGTTGACTTTCCCCAAAAGTCGACTCTCCCCGAATCTTGACAAGTCGGCCTACACCCTCCCGATGAAGGGGATAAGAATAGGCAACAACAAACTCAACATCTCGCCCGCCGTTTTCAAACCGGACCCATCTGGGGCCGGTCAGACCATGATCGACTCCGGCTCCGATTTGACGTATTTGGTAGATGAGGCCTACAGCAAGGTCAGAGAAGAGATGGTCAGATTAGTGGGGCCCATGATGAAGAAAGGATACGAATACGCCGCCGTCGCCGACATGTGTTTCGACGGCGCAGTGGCGGCGGCGGTGGGTCGGAGGATCGGCGACATGTGGTTCCAGTTTGAGAATGGGGTGGAGATATTGGTTGGGGAAAGGGGAAGGGTTATTGACGGAAGTGGAGGAAGGGGTGAAGTGCGTGGGGATCGGACGGTCAGATAG

mRNA sequence

ATGCCTCTCTCTCTCCTATTTCTCTCCCTATTCTGTCTCTTATTCTCCCCATCAAACTCCCTCTCCCTCTCCCTCCCTCTGACCTACCATTCTGTCTCCTCCCAAGAAGCCTCCCTTTCCCTCTCCTCCAAAACCAAATCCCATGGGAAGTTCCCCTTCCAGTACTCCAACGCCCTTGTGGTGTCTGTTCCCATTGGATCGCCGCCGCAGCAGATGGACATGGTCGTAGACACTGGCAGCCAACTGTCCTGGATTCAATGCCACGGCAAAGTAAGGAGAAAATCAGTGAAGCCCATGATTAATTGGTTTGACCCTTATCTCTCCTCCTCTTTCTCTTTCCTTCCCTGTAACACCACTCTGTGCAGACCCCGAATTCCCGATTTTACCCTTCCTACTTCTTGTGACCCAACTCGCCACTGCCACTACTCCTACTTCTACGCGGATGGGACCCTGGCCGAGGGTAATCTCGTCACTGAAAAATTCACCTTCTCCAATTCCTTAACTACCCGCTCCCTCCTTCTGGGCTGTGCTACGGCCTCCACCCAAAACAGGGGTATGTTGGGAATGAATACTGGACGCCTCTCCTTCATCTCCCAGGCCAAAATATCCAAGTTTTCCTATTGCGTTCCTGATCGAACCGGGTCGGATCTAACCGGCTTGTTCTACCTTGGAGACAACCCCAACTCCGCTAAATTCAAATACGTCAACATGTTGACTTTCCCCAAAAGTCGACTCTCCCCGAATCTTGACAAGTCGGCCTACACCCTCCCGATGAAGGGGATAAGAATAGGCAACAACAAACTCAACATCTCGCCCGCCGTTTTCAAACCGGACCCATCTGGGGCCGGTCAGACCATGATCGACTCCGGCTCCGATTTGACGTATTTGGTAGATGAGGCCTACAGCAAGGTCAGAGAAGAGATGGTCAGATTAGTGGGGCCCATGATGAAGAAAGGATACGAATACGCCGCCGTCGCCGACATGTGTTTCGACGGCGCAGTGGCGGCGGCGGTGGGTCGGAGGATCGGCGACATGTGGTTCCAGTTTGAGAATGGGGTGGAGATATTGGTTGGGGAAAGGGGAAGGGTTATTGACGGAAGTGGAGGAAGGGGTGAAGTGCGTGGGGATCGGACGGTCAGATAG

Coding sequence (CDS)

ATGCCTCTCTCTCTCCTATTTCTCTCCCTATTCTGTCTCTTATTCTCCCCATCAAACTCCCTCTCCCTCTCCCTCCCTCTGACCTACCATTCTGTCTCCTCCCAAGAAGCCTCCCTTTCCCTCTCCTCCAAAACCAAATCCCATGGGAAGTTCCCCTTCCAGTACTCCAACGCCCTTGTGGTGTCTGTTCCCATTGGATCGCCGCCGCAGCAGATGGACATGGTCGTAGACACTGGCAGCCAACTGTCCTGGATTCAATGCCACGGCAAAGTAAGGAGAAAATCAGTGAAGCCCATGATTAATTGGTTTGACCCTTATCTCTCCTCCTCTTTCTCTTTCCTTCCCTGTAACACCACTCTGTGCAGACCCCGAATTCCCGATTTTACCCTTCCTACTTCTTGTGACCCAACTCGCCACTGCCACTACTCCTACTTCTACGCGGATGGGACCCTGGCCGAGGGTAATCTCGTCACTGAAAAATTCACCTTCTCCAATTCCTTAACTACCCGCTCCCTCCTTCTGGGCTGTGCTACGGCCTCCACCCAAAACAGGGGTATGTTGGGAATGAATACTGGACGCCTCTCCTTCATCTCCCAGGCCAAAATATCCAAGTTTTCCTATTGCGTTCCTGATCGAACCGGGTCGGATCTAACCGGCTTGTTCTACCTTGGAGACAACCCCAACTCCGCTAAATTCAAATACGTCAACATGTTGACTTTCCCCAAAAGTCGACTCTCCCCGAATCTTGACAAGTCGGCCTACACCCTCCCGATGAAGGGGATAAGAATAGGCAACAACAAACTCAACATCTCGCCCGCCGTTTTCAAACCGGACCCATCTGGGGCCGGTCAGACCATGATCGACTCCGGCTCCGATTTGACGTATTTGGTAGATGAGGCCTACAGCAAGGTCAGAGAAGAGATGGTCAGATTAGTGGGGCCCATGATGAAGAAAGGATACGAATACGCCGCCGTCGCCGACATGTGTTTCGACGGCGCAGTGGCGGCGGCGGTGGGTCGGAGGATCGGCGACATGTGGTTCCAGTTTGAGAATGGGGTGGAGATATTGGTTGGGGAAAGGGGAAGGGTTATTGACGGAAGTGGAGGAAGGGGTGAAGTGCGTGGGGATCGGACGGTCAGATAG

Protein sequence

MPLSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALVVSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGERGRVIDGSGGRGEVRGDRTVR
Homology
BLAST of Csor.00g015030 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.0e-50
Identity = 123/340 (36.18%), Postives = 181/340 (53.24%), Query Frame = 0

Query: 12  CLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKT-----KSHGKFPFQYSNALVVSVPIG 71
           C+  S S+S S S      S SSQ   L L ++      +   K  F ++  L V++ +G
Sbjct: 21  CVSSSSSSSSSFSFSSFSSSSSSQTLVLPLKTRITPTDHRPTDKLHFHHNVTLTVTLTVG 80

Query: 72  SPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIP 131
           +PPQ + MV+DTGS+LSW++C+    R S    +N FDP  SSS+S +PC++  CR R  
Sbjct: 81  TPPQNISMVIDTGSELSWLRCN----RSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTR 140

Query: 132 DFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATA------- 191
           DF +P SCD  + CH +  YAD + +EGNL  E F F NS    +L+ GC  +       
Sbjct: 141 DFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPE 200

Query: 192 -STQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNML 251
             T+  G+LGMN G LSFISQ    KFSYC+      D  G   LGD    + F ++  L
Sbjct: 201 EDTKTTGLLGMNRGSLSFISQMGFPKFSYCISGT--DDFPGFLLLGD----SNFTWLTPL 260

Query: 252 TF-PKSRLS---PNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLT 311
            + P  R+S   P  D+ AYT+ + GI++    L I  +V  PD +GAGQTM+DSG+  T
Sbjct: 261 NYTPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFT 320

Query: 312 YLVDEAYSKVREEMVRLVGPMM----KKGYEYAAVADMCF 331
           +L+   Y+ +R   +     ++       + +    D+C+
Sbjct: 321 FLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCY 350

BLAST of Csor.00g015030 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.9e-29
Identity = 87/277 (31.41%), Postives = 137/277 (49.46%), Query Frame = 0

Query: 60  VVSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTT 119
           ++++ IG+P Q    ++DTGS L W QC    +  +    I  F+P  SSSFS LPC++ 
Sbjct: 96  LMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPI--FNPQGSSSFSTLPCSSQ 155

Query: 120 LCRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCAT- 179
           LC+       L +       C Y+Y Y DG+  +G++ TE  TF  S++  ++  GC   
Sbjct: 156 LCQ------ALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTF-GSVSIPNITFGCGEN 215

Query: 180 ----ASTQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKY 239
                     G++GM  G LS  SQ  ++KFSYC+    GS       LG   NS     
Sbjct: 216 NQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTP-IGSSTPSNLLLGSLANSVTAGS 275

Query: 240 VNMLTFPKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDP-SGAGQTMIDSGSDL 299
            N      S++      + Y + + G+ +G+ +L I P+ F  +  +G G  +IDSG+ L
Sbjct: 276 PNTTLIQSSQI-----PTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTL 335

Query: 300 TYLVDEAYSKVREEMVRLVGPMMKKGYEYAAVADMCF 331
           TY V+ AY  VR+E +  +   +  G   ++  D+CF
Sbjct: 336 TYFVNNAYQSVRQEFISQINLPVVNG--SSSGFDLCF 355

BLAST of Csor.00g015030 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 2.1e-27
Identity = 95/319 (29.78%), Postives = 149/319 (46.71%), Query Frame = 0

Query: 60  VVSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTT 119
           +++V IG+P      ++DTGS L W QC    +  S    I  F+P  SSSFS LPC + 
Sbjct: 97  LMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPI--FNPQDSSSFSTLPCESQ 156

Query: 120 LCRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCAT- 179
            C+       LP+       C Y+Y Y DG+  +G + TE FTF  S +  ++  GC   
Sbjct: 157 YCQ------DLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETS-SVPNIAFGCGED 216

Query: 180 ----ASTQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKY 239
                     G++GM  G LS  SQ  + +FSYC+    GS       LG   +    + 
Sbjct: 217 NQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTS-YGSSSPSTLALGSAASGVP-EG 276

Query: 240 VNMLTFPKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLT 299
               T   S L+P    + Y + ++GI +G + L I  + F+    G G  +IDSG+ LT
Sbjct: 277 SPSTTLIHSSLNP----TYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLT 336

Query: 300 YLVDEAYSKVREEMVRLVGPMMKKGYEYAAVADMCF----DGAVAAAVGRRIGDMWFQFE 359
           YL  +AY+ V +     +   +    E ++    CF    DG+       ++ ++  QF+
Sbjct: 337 YLPQDAYNAVAQAFTDQIN--LPTVDESSSGLSTCFQQPSDGSTV-----QVPEISMQFD 392

Query: 360 NGVEILVGERGRVIDGSGG 370
            GV + +GE+  +I  + G
Sbjct: 397 GGV-LNLGEQNILISPAEG 392

BLAST of Csor.00g015030 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 3.6e-27
Identity = 91/312 (29.17%), Postives = 153/312 (49.04%), Query Frame = 0

Query: 63  VPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCR 122
           + +G+P ++M +V+DTGS ++WIQC            +  F+P  SS++  L C+   C 
Sbjct: 166 IGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPV--FNPTSSSTYKSLTCSAPQCS 225

Query: 123 PRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS-- 182
                  L TS   +  C Y   Y DG+   G L T+  TF NS    ++ LGC   +  
Sbjct: 226 ------LLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEG 285

Query: 183 --TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNML 242
             T   G+LG+  G LS  +Q K + FSYC+ DR     + L +     NS +    +  
Sbjct: 286 LFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSLDF-----NSVQLGGGD-A 345

Query: 243 TFPKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVD 302
           T P  R +  +D + Y + + G  +G  K+ +  A+F  D SG+G  ++D G+ +T L  
Sbjct: 346 TAPLLR-NKKID-TFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQT 405

Query: 303 EAYSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVG 362
           +AY+ +R+  ++L    +KKG    ++ D C+D +  + V  ++  + F F  G  + + 
Sbjct: 406 QAYNSLRDAFLKLT-VNLKKGSSSISLFDTCYDFSSLSTV--KVPTVAFHFTGGKSLDLP 458

Query: 363 ERGRVI--DGSG 369
            +  +I  D SG
Sbjct: 466 AKNYLIPVDDSG 458

BLAST of Csor.00g015030 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.8e-24
Identity = 87/287 (30.31%), Postives = 142/287 (49.48%), Query Frame = 0

Query: 54  QYSNALVVSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSF 113
           Q S      + +G+P + + MV+DTGS + W+QC    R  S    I  FDP  S +++ 
Sbjct: 137 QGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPI--FDPRKSKTYAT 196

Query: 114 LPCNTTLCRPRIPDFTLPTSCDPTRH-CHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSL 173
           +PC++  CR R+        C+  R  C Y   Y DG+   G+  TE  TF  +   + +
Sbjct: 197 IPCSSPHCR-RLDS----AGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRN-RVKGV 256

Query: 174 LLGCATAS----TQNRGMLGMNTGRLSFISQAK---ISKFSYCVPDRTGSDLTGLFYLGD 233
            LGC   +        G+LG+  G+LSF  Q       KFSYC+ DR+ S        G+
Sbjct: 257 ALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGN 316

Query: 234 NPNSAKFKYVNMLTFPKSRLSPNLDKSAYTLPMKGIRIGNNKL-NISPAVFKPDPSGAGQ 293
              S   ++  +L+ PK      LD + Y + + GI +G  ++  ++ ++FK D  G G 
Sbjct: 317 AAVSRIARFTPLLSNPK------LD-TFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGG 376

Query: 294 TMIDSGSDLTYLVDEAYSKVREEMVRLVGPMMKKGYEYAAVADMCFD 332
            +IDSG+ +T L+  AY  +R+   R+    +K+  ++ ++ D CFD
Sbjct: 377 VIIDSGTSVTRLIRPAYIAMRDAF-RVGAKTLKRAPDF-SLFDTCFD 406

BLAST of Csor.00g015030 vs. NCBI nr
Match: KAG6607570.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 758 bits (1956), Expect = 4.97e-276
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MPLSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGER 360
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGER
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGER 360

Query: 361 GRVIDGSGGRGEVRGDRTVR 380
           GRVIDGSGGRGEVRGDRTVR
Sbjct: 361 GRVIDGSGGRGEVRGDRTVR 380

BLAST of Csor.00g015030 vs. NCBI nr
Match: KAG6607571.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 716 bits (1849), Expect = 4.53e-259
Identity = 358/359 (99.72%), Postives = 359/359 (100.00%), Query Frame = 0

Query: 1   MPLSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGE 359
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVG+
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGK 359

BLAST of Csor.00g015030 vs. NCBI nr
Match: KAG6607573.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 706 bits (1821), Expect = 8.36e-255
Identity = 354/359 (98.61%), Postives = 355/359 (98.89%), Query Frame = 0

Query: 1   MPLSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLL LSL  LLFSPSNSLSLS PLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGE 359
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVG+
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGK 359

BLAST of Csor.00g015030 vs. NCBI nr
Match: XP_022932084.1 (aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022932086.1 aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022932444.1 aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022933293.1 aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022933314.1 aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 702 bits (1812), Expect = 1.96e-253
Identity = 353/359 (98.33%), Postives = 354/359 (98.61%), Query Frame = 0

Query: 1   MPLSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLL LSL  LLFSPSNSLSLS PLT HSVSSQEASLSLSSKTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGE 359
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVG+
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGK 359

BLAST of Csor.00g015030 vs. NCBI nr
Match: XP_022932083.1 (aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 702 bits (1811), Expect = 2.79e-253
Identity = 352/359 (98.05%), Postives = 354/359 (98.61%), Query Frame = 0

Query: 1   MPLSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLL LSL  LLFSPSNSLSLS PLT HSVSSQEASLSLSSKTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPA+FKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAIFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGE 359
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVG+
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGK 359

BLAST of Csor.00g015030 vs. ExPASy TrEMBL
Match: A0A6J1EWZ6 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438863 PE=3 SV=1)

HSP 1 Score: 702 bits (1812), Expect = 9.51e-254
Identity = 353/359 (98.33%), Postives = 354/359 (98.61%), Query Frame = 0

Query: 1   MPLSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLL LSL  LLFSPSNSLSLS PLT HSVSSQEASLSLSSKTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGE 359
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVG+
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGK 359

BLAST of Csor.00g015030 vs. ExPASy TrEMBL
Match: A0A6J1F0M7 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438404 PE=3 SV=1)

HSP 1 Score: 702 bits (1811), Expect = 1.35e-253
Identity = 352/359 (98.05%), Postives = 354/359 (98.61%), Query Frame = 0

Query: 1   MPLSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLL LSL  LLFSPSNSLSLS PLT HSVSSQEASLSLSSKTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPA+FKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAIFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGE 359
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVG+
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGK 359

BLAST of Csor.00g015030 vs. ExPASy TrEMBL
Match: A0A6J1F181 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438406 PE=3 SV=1)

HSP 1 Score: 701 bits (1808), Expect = 3.87e-253
Identity = 352/359 (98.05%), Postives = 354/359 (98.61%), Query Frame = 0

Query: 1   MPLSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLL LSL  LLFSPSNSLSLS PLT HSVSS+EASLSLSSKTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSREASLSLSSKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGE 359
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVG+
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGK 359

BLAST of Csor.00g015030 vs. ExPASy TrEMBL
Match: A0A6J1F1P3 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438856 PE=3 SV=1)

HSP 1 Score: 700 bits (1806), Expect = 7.80e-253
Identity = 352/359 (98.05%), Postives = 353/359 (98.33%), Query Frame = 0

Query: 1   MPLSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLL LSL  LLFSPSNSLSLS PLT HSVSSQEASLSLS KTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSIKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGE 359
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVG+
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGK 359

BLAST of Csor.00g015030 vs. ExPASy TrEMBL
Match: A0A6J1I612 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111471392 PE=3 SV=1)

HSP 1 Score: 691 bits (1782), Expect = 3.53e-249
Identity = 347/359 (96.66%), Postives = 350/359 (97.49%), Query Frame = 0

Query: 1   MPLSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLL LSLF +LFSPSNSLSLS PLT HSVSSQEASLSLSSKTKSHGK PFQYSNALV
Sbjct: 1   MPLSLLLLSLFGVLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKLPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFS+SLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSSSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSR SPNLDK AYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRRSPNLDKLAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGE 359
           YSKVR EMVRLVGPMMKKGYEYAAVADMCFDGAVAA VGRRIGDMWFQFENGVEILVG+
Sbjct: 301 YSKVRAEMVRLVGPMMKKGYEYAAVADMCFDGAVAAVVGRRIGDMWFQFENGVEILVGK 359

BLAST of Csor.00g015030 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 418.7 bits (1075), Expect = 5.0e-117
Identity = 221/381 (58.01%), Postives = 273/381 (71.65%), Query Frame = 0

Query: 3   LSLLFLSLFCLLFSPSNSLSLSLPLTYHSVSSQEASLS-----LSSKTKSHGKFPF---- 62
           L + F   + +  S S+SLSL  PLT   ++    S S     LS +  S    P+    
Sbjct: 13  LYIFFFFCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPPSSPYTFRS 72

Query: 63  --QYSNALVVSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSF 122
             +YS AL++S+PIG+P Q  ++V+DTGSQLSWIQCH K  +K + P    FDP LSSSF
Sbjct: 73  NIKYSMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPPPTTSFDPSLSSSF 132

Query: 123 SFLPCNTTLCRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRS 182
           S LPC+  LC+PRIPDFTLPTSCD  R CHYSYFYADGT AEGNLV EKFTFSNS TT  
Sbjct: 133 SDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTFSNSQTTPP 192

Query: 183 LLLGCATASTQNRGMLGMNTGRLSFISQAKISKFSYCVP---DRTGSDLTGLFYLGDNPN 242
           L+LGCA  ST  +G+LGMN GRLSFISQAKISKFSYC+P   +R G   TG FYLGDNPN
Sbjct: 193 LILGCAKESTDEKGILGMNLGRLSFISQAKISKFSYCIPTRSNRPGLASTGSFYLGDNPN 252

Query: 243 SAKFKYVNMLTFPKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMID 302
           S  FKYV++LTFP+S+  PNLD  AYT+P++GIRIG  +LNI  +VF+PD  G+GQTM+D
Sbjct: 253 SRGFKYVSLLTFPQSQRMPNLDPLAYTVPLQGIRIGQKRLNIPGSVFRPDAGGSGQTMVD 312

Query: 303 SGSDLTYLVDEAYSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQ 362
           SGS+ T+LVD AY KV+EE+VRLVG  +KKGY Y + ADMCFDG  +  +GR IGD+ F+
Sbjct: 313 SGSEFTHLVDVAYDKVKEEIVRLVGSRLKKGYVYGSTADMCFDGNHSMEIGRLIGDLVFE 372

Query: 363 FENGVEILVGERGRVIDGSGG 370
           F  GVEILV ++  +++  GG
Sbjct: 373 FGRGVEILVEKQSLLVNVGGG 393

BLAST of Csor.00g015030 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 403.7 bits (1036), Expect = 1.7e-112
Identity = 223/378 (58.99%), Postives = 264/378 (69.84%), Query Frame = 0

Query: 6   LFLSLFCLLFSPSNSLSLSLPLTYHSVS----SQEASLSLSSKTKSHGKFP-------FQ 65
           LF   F    S S SLSL LPLT   +S    S   + SL S+       P       F+
Sbjct: 8   LFFFFFLNYVSLSTSLSLHLPLTSLPISTTTNSHRFTTSLLSRKNPSPSSPPYNFRSRFK 67

Query: 66  YSNALVVSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFL 125
           YS AL++S+PIG+PPQ   MV+DTGSQLSWIQCH K  +   KP  + FDP LSSSFS L
Sbjct: 68  YSMALIISLPIGTPPQAQQMVLDTGSQLSWIQCHRK--KLPPKPKTS-FDPSLSSSFSTL 127

Query: 126 PCNTTLCRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLL 185
           PC+  LC+PRIPDFTLPTSCD  R CHYSYFYADGT AEGNLV EK TFSN+  T  L+L
Sbjct: 128 PCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSNTEITPPLIL 187

Query: 186 GCATASTQNRGMLGMNTGRLSFISQAKISKFSYCVP---DRTGSDLTGLFYLGDNPNSAK 245
           GCAT S+ +RG+LGMN GRLSF+SQAKISKFSYC+P   +R G   TG FYLGDNPNS  
Sbjct: 188 GCATESSDDRGILGMNRGRLSFVSQAKISKFSYCIPPKSNRPGFTPTGSFYLGDNPNSHG 247

Query: 246 FKYVNMLTFPKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGS 305
           FKYV++LTFP+S+  PNLD  AYT+PM GIR G  KLNIS +VF+PD  G+GQTM+DSGS
Sbjct: 248 FKYVSLLTFPESQRMPNLDPLAYTVPMIGIRFGLKKLNISGSVFRPDAGGSGQTMVDSGS 307

Query: 306 DLTYLVDEAYSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFEN 365
           + T+LVD AY KVR E++  VG  +KKGY Y   ADMCFDG V A + R IGD+ F F  
Sbjct: 308 EFTHLVDAAYDKVRAEIMTRVGRRLKKGYVYGGTADMCFDGNV-AMIPRLIGDLVFVFTR 367

Query: 366 GVEILVGERGRVIDGSGG 370
           GVEILV +   +++  GG
Sbjct: 368 GVEILVPKERVLVNVGGG 381

BLAST of Csor.00g015030 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 203.8 bits (517), Expect = 2.5e-52
Identity = 124/349 (35.53%), Postives = 189/349 (54.15%), Query Frame = 0

Query: 16  SPSNSLSLS------------LPLTYHSVSSQEASLSLSSKTK-----SHGKFPFQYSNA 75
           S S+SLSLS             PLT+   SS   +L  S KT+     S  K  F+++  
Sbjct: 5   SSSSSLSLSKNFLRISVLLLIFPLTFCKTSSTNQTLLFSLKTQKLPQSSSDKLSFRHNVT 64

Query: 76  LVVSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNT 135
           L V++ +G PPQ + MV+DTGS+LSW+ C       SV      F+P  SS++S +PC++
Sbjct: 65  LTVTLAVGDPPQNISMVLDTGSELSWLHCKKSPNLGSV------FNPVSSSTYSPVPCSS 124

Query: 136 TLCRPRIPDFTLPTSCDPTRH-CHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGC- 195
            +CR R  D  +P SCDP  H CH +  YAD T  EGNL  E F    S+T    L GC 
Sbjct: 125 PICRTRTRDLPIPASCDPKTHLCHVAISYADATSIEGNLAHETFVI-GSVTRPGTLFGCM 184

Query: 196 -------ATASTQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNS 255
                  +    ++ G++GMN G LSF++Q   SKFSYC+   +GSD +G   LGD    
Sbjct: 185 DSGLSSNSEEDAKSTGLMGMNRGSLSFVNQLGFSKFSYCI---SGSDSSGFLLLGD---- 244

Query: 256 AKFKYVNMLTFP----KSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQT 315
           A + ++  + +     +S   P  D+ AYT+ ++GIR+G+  L++  +VF PD +GAGQT
Sbjct: 245 ASYSWLGPIQYTPLVLQSTPLPYFDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQT 304

Query: 316 MIDSGSDLTYLVDEAYSKVREEMVRLVGPMMK----KGYEYAAVADMCF 331
           M+DSG+  T+L+   Y+ ++ E +     +++      + +    D+C+
Sbjct: 305 MVDSGTQFTFLMGPVYTALKNEFITQTKSVLRLVDDPDFVFQGTMDLCY 339

BLAST of Csor.00g015030 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 202.2 bits (513), Expect = 7.4e-52
Identity = 123/340 (36.18%), Postives = 181/340 (53.24%), Query Frame = 0

Query: 12  CLLFSPSNSLSLSLPLTYHSVSSQEASLSLSSKT-----KSHGKFPFQYSNALVVSVPIG 71
           C+  S S+S S S      S SSQ   L L ++      +   K  F ++  L V++ +G
Sbjct: 21  CVSSSSSSSSSFSFSSFSSSSSSQTLVLPLKTRITPTDHRPTDKLHFHHNVTLTVTLTVG 80

Query: 72  SPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIP 131
           +PPQ + MV+DTGS+LSW++C+    R S    +N FDP  SSS+S +PC++  CR R  
Sbjct: 81  TPPQNISMVIDTGSELSWLRCN----RSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTR 140

Query: 132 DFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATA------- 191
           DF +P SCD  + CH +  YAD + +EGNL  E F F NS    +L+ GC  +       
Sbjct: 141 DFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPE 200

Query: 192 -STQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNML 251
             T+  G+LGMN G LSFISQ    KFSYC+      D  G   LGD    + F ++  L
Sbjct: 201 EDTKTTGLLGMNRGSLSFISQMGFPKFSYCISGT--DDFPGFLLLGD----SNFTWLTPL 260

Query: 252 TF-PKSRLS---PNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLT 311
            + P  R+S   P  D+ AYT+ + GI++    L I  +V  PD +GAGQTM+DSG+  T
Sbjct: 261 NYTPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFT 320

Query: 312 YLVDEAYSKVREEMVRLVGPMM----KKGYEYAAVADMCF 331
           +L+   Y+ +R   +     ++       + +    D+C+
Sbjct: 321 FLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCY 350

BLAST of Csor.00g015030 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 131.0 bits (328), Expect = 2.1e-30
Identity = 106/341 (31.09%), Postives = 157/341 (46.04%), Query Frame = 0

Query: 44  KTKSHGKFPFQYSNALVVSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWF 103
           K  +HG      S   ++ + IG+P  +   +VDTGS L W QC  K   +        F
Sbjct: 97  KAPTHGG-----SGEFLMELSIGNPAVKYSAIVDTGSDLIWTQC--KPCTECFDQPTPIF 156

Query: 104 DPYLSSSFSFLPCNTTLCRPRIPDFTLPTS-CDPTRH-CHYSYFYADGTLAEGNLVTEKF 163
           DP  SSS+S + C++ LC        LP S C+  +  C Y Y Y D +   G L TE F
Sbjct: 157 DPEKSSSYSKVGCSSGLCN------ALPRSNCNEDKDACEYLYTYGDYSSTRGLLATETF 216

Query: 164 TFSNSLTTRSLLLGCATAS-----TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSD 223
           TF +  +   +  GC   +     +Q  G++G+  G LS ISQ K +KFSYC+     S+
Sbjct: 217 TFEDENSISGIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQLKETKFSYCLTSIEDSE 276

Query: 224 LTGLFYLGDNP----NSAKFKYVNMLTFPKSRLSPNLDKSAYTLPMKGIRIGNNKLNISP 283
            +   ++G       N         +T   S L      S Y L ++GI +G  +L++  
Sbjct: 277 ASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQGITVGAKRLSVEK 336

Query: 284 AVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMV-RLVGPMMKKGYEYAAVADMCF- 343
           + F+    G G  +IDSG+ +TYL + A+  ++EE   R+  P+   G   +   D+CF 
Sbjct: 337 STFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPVDDSG---STGLDLCFK 396

Query: 344 --DGAVAAAVGRRIGDMWFQFENGVEILVGERGRVIDGSGG 370
             D A   AV + I    F F+     L GE   V D S G
Sbjct: 397 LPDAAKNIAVPKMI----FHFKGADLELPGENYMVADSSTG 417

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZL31.0e-5036.18Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1[more]
Q766C32.9e-2931.41Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C22.1e-2729.78Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q9LS403.6e-2729.17Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LNJ32.8e-2430.31Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Match NameE-valueIdentityDescription
KAG6607570.14.97e-276100.00Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6607571.14.53e-25999.72Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6607573.18.36e-25598.61Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022932084.11.96e-25398.33aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022932086.1 aspartic prot... [more]
XP_022932083.12.79e-25398.05aspartic proteinase PCS1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1EWZ69.51e-25498.33aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438863 PE=3... [more]
A0A6J1F0M71.35e-25398.05aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438404 PE=3... [more]
A0A6J1F1813.87e-25398.05aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438406 PE=3... [more]
A0A6J1F1P37.80e-25398.05aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438856 PE=3... [more]
A0A6J1I6123.53e-24996.66aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111471392 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT5G37540.15.0e-11758.01Eukaryotic aspartyl protease family protein [more]
AT1G66180.11.7e-11258.99Eukaryotic aspartyl protease family protein [more]
AT2G39710.12.5e-5235.53Eukaryotic aspartyl protease family protein [more]
AT5G02190.17.4e-5236.18Eukaryotic aspartyl protease family protein [more]
AT2G03200.12.1e-3031.09Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 29..224
e-value: 1.4E-32
score: 115.1
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 225..365
e-value: 2.8E-20
score: 74.5
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 60..367
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 253..355
e-value: 6.6E-15
score: 55.3
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 60..225
e-value: 2.1E-34
score: 119.3
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 24..364
NoneNo IPR availablePANTHERPTHR47965:SF51EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 24..364
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 74..85
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 59..380
score: 21.02124
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 60..304
e-value: 2.26099E-54
score: 178.611

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g015030.m01Csor.00g015030.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity