Csor.00g010230 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g010230
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	beta-amyrin 11-oxidase-like
Location	Csor_Chr16: 7881166 .. 7881486 (-)
RNA-Seq Expression	Csor.00g010230
Synteny	Csor.00g010230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglepolypeptidestart_codonstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTCATAAATGGGGTTTTCTTGATTCTTCCTTTGGGCTTTGTTTTAGTTTTTGAGGTTTTGAAGAGATTGAATGGTTTGTATTATGCTGTGAAGTTGGGGAAGAAATGGGGTGAGCTTCCTCCTGGTGATCTCTCTTGGCCACTTCTTGGTTCTACTTTATCCTTCCTCAAATCTTTCACGGTTGGTCCACCTGAAAGCTTTATTCGTATATCATGTCTATTTGATCATAATTCAACAAAATATTCGTTGTTCTACCAAAATGCCTCTAATTTTGGGATGGCTGACTTAATGATTGCAATAGCTAGTTCAGAATAA

mRNA sequence

ATGGAGTTCATAAATGGGGTTTTCTTGATTCTTCCTTTGGGCTTTGTTTTAGTTTTTGAGGTTTTGAAGAGATTGAATGGTTTGTATTATGCTGTGAAGTTGGGGAAGAAATGGGGTGAGCTTCCTCCTGGTGATCTCTCTTGGCCACTTCTTGGTTCTACTTTATCCTTCCTCAAATCTTTCACGGTTGGTCCACCTGAAAGCTTTATTCGTATATCATGTCTATTTGATCATAATTCAACAAAATATTCGTTGTTCTACCAAAATGCCTCTAATTTTGGGATGGCTGACTTAATGATTGCAATAGCTAGTTCAGAATAA

Coding sequence (CDS)

ATGGAGTTCATAAATGGGGTTTTCTTGATTCTTCCTTTGGGCTTTGTTTTAGTTTTTGAGGTTTTGAAGAGATTGAATGGTTTGTATTATGCTGTGAAGTTGGGGAAGAAATGGGGTGAGCTTCCTCCTGGTGATCTCTCTTGGCCACTTCTTGGTTCTACTTTATCCTTCCTCAAATCTTTCACGGTTGGTCCACCTGAAAGCTTTATTCGTATATCATGTCTATTTGATCATAATTCAACAAAATATTCGTTGTTCTACCAAAATGCCTCTAATTTTGGGATGGCTGACTTAATGATTGCAATAGCTAGTTCAGAATAA

Protein sequence

MEFINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKSFTVGPPESFIRISCLFDHNSTKYSLFYQNASNFGMADLMIAIASSE

Homology

[Close]

BLAST of Csor.00g010230 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 5.5e-10
Identity = 28/56 (50.00%), Postives = 39/56 (69.64%), Query Frame = 0


Query: 15 FVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKSFTVGPPESFI 71
          ++   + ++ LNG YY VKL +K   LPPGD+ WPL+G  LSF+K F+ G P+SFI
Sbjct: 17 YIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFIKDFSSGHPDSFI 72

[Close]

BLAST of Csor.00g010230 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 1.6e-09
Identity = 31/64 (48.44%), Postives = 43/64 (67.19%), Query Frame = 0


Query: 8  FLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKSFTVGPPE 67
          F ++ LG  ++  VLKR+N   Y  KLG+K   LPPGDL WP++G+  SFL++F    PE
Sbjct: 11 FPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPE 70

Query: 68 SFIR 72
          SFI+
Sbjct: 71 SFIQ 74

[Close]

BLAST of Csor.00g010230 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.8e-06
Identity = 25/64 (39.06%), Postives = 39/64 (60.94%), Query Frame = 0


Query: 8  FLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKSFTVGPPE 67
          F ++ LG   +  +++++N   Y   LG+    LPPGDL WP +G+ LSFL++F    P+
Sbjct: 12 FPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPD 71

Query: 68 SFIR 72
          SF R
Sbjct: 72 SFTR 75

[Close]

BLAST of Csor.00g010230 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 2.4e-05
Identity = 23/63 (36.51%), Postives = 37/63 (58.73%), Query Frame = 0


Query: 9  LILPLGFVLVFEVLKRLNGLYYAVKLGK-KWGELPPGDLSWPLLGSTLSFLKSFTVGPPE 68
          ++  L  V +   ++  +  Y+   LG  + G LPPGD+ WPL+G   +FL++F  G P+
Sbjct: 12 VVAALAVVALDAAVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFKSGRPD 71

Query: 69 SFI 71
          SFI
Sbjct: 72 SFI 74

[Close]

BLAST of Csor.00g010230 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 4.1e-05
Identity = 23/58 (39.66%), Postives = 37/58 (63.79%), Query Frame = 0


Query: 14 GFVLVFEVLKRLNGLYYAVKLG-KKWGELPPGDLSWPLLGSTLSFLKSFTVGPPESFI 71
          G VLV  V++R +       LG ++   LPPG++ WP++GS  +FL++F  G P++FI
Sbjct: 27 GVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKSGNPDAFI 84

[Close]

BLAST of Csor.00g010230 vs. NCBI nr
Match: KAG6577617.1 (Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 214 bits (546), Expect = 3.55e-70
Identity = 106/106 (100.00%), Postives = 106/106 (100.00%), Query Frame = 0


Query: 1   MEFINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
           MEFINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS
Sbjct: 1   MEFINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60

Query: 61  FTVGPPESFIRISCLFDHNSTKYSLFYQNASNFGMADLMIAIASSE 106
           FTVGPPESFIRISCLFDHNSTKYSLFYQNASNFGMADLMIAIASSE
Sbjct: 61  FTVGPPESFIRISCLFDHNSTKYSLFYQNASNFGMADLMIAIASSE 106

[Close]

BLAST of Csor.00g010230 vs. NCBI nr
Match: KAG6577628.1 (Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 145 bits (366), Expect = 7.13e-39
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 1  MEFINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
          MEFINGVFLILPLGFVLVFEVLKRLNGLYYA+KLGKKWGELPPGDLSWPLLGSTLSFLKS
Sbjct: 1  MEFINGVFLILPLGFVLVFEVLKRLNGLYYALKLGKKWGELPPGDLSWPLLGSTLSFLKS 60

Query: 61 FTVGPPESFIR 71
          FTVGPPESFIR
Sbjct: 61 FTVGPPESFIR 71

[Close]

BLAST of Csor.00g010230 vs. NCBI nr
Match: XP_023007801.1 (beta-amyrin 11-oxidase-like [Cucurbita maxima])

HSP 1 Score: 145 bits (366), Expect = 9.13e-39
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 1  MEFINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
          MEFINGVFLILPLGFVLVFEVLKRLNGLYYA+KLGKKWGELPPGDLSWPLLGSTLSFLKS
Sbjct: 1  MEFINGVFLILPLGFVLVFEVLKRLNGLYYALKLGKKWGELPPGDLSWPLLGSTLSFLKS 60

Query: 61 FTVGPPESFIR 71
          FTVGPPESFIR
Sbjct: 61 FTVGPPESFIR 71

[Close]

BLAST of Csor.00g010230 vs. NCBI nr
Match: XP_023552973.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 145 bits (366), Expect = 9.13e-39
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 1  MEFINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
          MEFINGVFLILPLGFVLVFEVLKRLNGLYYA+KLGKKWGELPPGDLSWPLLGSTLSFLKS
Sbjct: 1  MEFINGVFLILPLGFVLVFEVLKRLNGLYYALKLGKKWGELPPGDLSWPLLGSTLSFLKS 60

Query: 61 FTVGPPESFIR 71
          FTVGPPESFIR
Sbjct: 61 FTVGPPESFIR 71

[Close]

BLAST of Csor.00g010230 vs. NCBI nr
Match: XP_023007793.1 (beta-amyrin 11-oxidase-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 143 bits (361), Expect = 4.84e-38
Identity = 69/71 (97.18%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MEFINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
          MEFINGVFLILP+GFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDL WPLLGSTLSFLKS
Sbjct: 1  MEFINGVFLILPVGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLCWPLLGSTLSFLKS 60

Query: 61 FTVGPPESFIR 71
          FTVGPPESFIR
Sbjct: 61 FTVGPPESFIR 71

[Close]

BLAST of Csor.00g010230 vs. ExPASy TrEMBL
Match: A0A6J1L1P3 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111500300 PE=3 SV=1)

HSP 1 Score: 145 bits (366), Expect = 4.42e-39
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 1  MEFINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
          MEFINGVFLILPLGFVLVFEVLKRLNGLYYA+KLGKKWGELPPGDLSWPLLGSTLSFLKS
Sbjct: 1  MEFINGVFLILPLGFVLVFEVLKRLNGLYYALKLGKKWGELPPGDLSWPLLGSTLSFLKS 60

Query: 61 FTVGPPESFIR 71
          FTVGPPESFIR
Sbjct: 61 FTVGPPESFIR 71

[Close]

BLAST of Csor.00g010230 vs. ExPASy TrEMBL
Match: A0A6J1L8M7 (beta-amyrin 11-oxidase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111500295 PE=3 SV=1)

HSP 1 Score: 143 bits (361), Expect = 2.34e-38
Identity = 69/71 (97.18%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MEFINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
          MEFINGVFLILP+GFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDL WPLLGSTLSFLKS
Sbjct: 1  MEFINGVFLILPVGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLCWPLLGSTLSFLKS 60

Query: 61 FTVGPPESFIR 71
          FTVGPPESFIR
Sbjct: 61 FTVGPPESFIR 71

[Close]

BLAST of Csor.00g010230 vs. ExPASy TrEMBL
Match: A0A6J1KZN6 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500295 PE=3 SV=1)

HSP 1 Score: 143 bits (361), Expect = 2.51e-38
Identity = 69/71 (97.18%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MEFINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
          MEFINGVFLILP+GFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDL WPLLGSTLSFLKS
Sbjct: 1  MEFINGVFLILPVGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLCWPLLGSTLSFLKS 60

Query: 61 FTVGPPESFIR 71
          FTVGPPESFIR
Sbjct: 61 FTVGPPESFIR 71

[Close]

BLAST of Csor.00g010230 vs. ExPASy TrEMBL
Match: A0A6J1E6E2 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431022 PE=3 SV=1)

HSP 1 Score: 142 bits (357), Expect = 8.89e-38
Identity = 69/71 (97.18%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MEFINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
          MEFINGVFLILPLGFVLVFEVLKRLNGLYYA+KLGKKW ELPPGDLSWPLLGSTLSFLKS
Sbjct: 1  MEFINGVFLILPLGFVLVFEVLKRLNGLYYALKLGKKWVELPPGDLSWPLLGSTLSFLKS 60

Query: 61 FTVGPPESFIR 71
          FTVGPPESFIR
Sbjct: 61 FTVGPPESFIR 71

[Close]

BLAST of Csor.00g010230 vs. ExPASy TrEMBL
Match: A0A6J1EBC2 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431021 PE=3 SV=1)

HSP 1 Score: 137 bits (345), Expect = 4.81e-36
Identity = 64/71 (90.14%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MEFINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
          MEFIN VFL+LP+GFVLV+EVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS
Sbjct: 1  MEFINWVFLVLPVGFVLVYEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60

Query: 61 FTVGPPESFIR 71
          FTVGPP+++IR
Sbjct: 61 FTVGPPQNYIR 71

[Close]

BLAST of Csor.00g010230 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 63.5 bits (153), Expect = 1.1e-10
Identity = 31/64 (48.44%), Postives = 43/64 (67.19%), Query Frame = 0


Query: 8  FLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKSFTVGPPE 67
          F ++ LG  ++  VLKR+N   Y  KLG+K   LPPGDL WP++G+  SFL++F    PE
Sbjct: 11 FPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPE 70

Query: 68 SFIR 72
          SFI+
Sbjct: 71 SFIQ 74

[Close]

BLAST of Csor.00g010230 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 63.5 bits (153), Expect = 1.1e-10
Identity = 31/64 (48.44%), Postives = 43/64 (67.19%), Query Frame = 0


Query: 8  FLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKSFTVGPPE 67
          F ++ LG  ++  VLKR+N   Y  KLG+K   LPPGDL WP++G+  SFL++F    PE
Sbjct: 11 FPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPE 70

Query: 68 SFIR 72
          SFI+
Sbjct: 71 SFIQ 74

[Close]

BLAST of Csor.00g010230 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 52.8 bits (125), Expect = 2.0e-07
Identity = 25/64 (39.06%), Postives = 39/64 (60.94%), Query Frame = 0


Query: 8  FLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKSFTVGPPE 67
          F ++ LG   +  +++++N   Y   LG+    LPPGDL WP +G+ LSFL++F    P+
Sbjct: 12 FPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPD 71

Query: 68 SFIR 72
          SF R
Sbjct: 72 SFTR 75

The following BLAST results are available for this feature:

BLAST of Csor.00g010230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	5.5e-10	50.00	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
Q9C5Y2	1.6e-09	48.44	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
O23051	2.8e-06	39.06	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]
Q9AXH9	2.4e-05	36.51	Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1	[more]
Q5VRM7	4.1e-05	39.66	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]

BLAST of Csor.00g010230 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6577617.1	3.55e-70	100.00	Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG6577628.1	7.13e-39	98.59	Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023007801.1	9.13e-39	98.59	beta-amyrin 11-oxidase-like [Cucurbita maxima]	[more]
XP_023552973.1	9.13e-39	98.59	beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo]	[more]
XP_023007793.1	4.84e-38	97.18	beta-amyrin 11-oxidase-like isoform X2 [Cucurbita maxima]	[more]

BLAST of Csor.00g010230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L1P3	4.42e-39	98.59	beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111500300 PE=3 SV=...	[more]
A0A6J1L8M7	2.34e-38	97.18	beta-amyrin 11-oxidase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1115002...	[more]
A0A6J1KZN6	2.51e-38	97.18	beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1115002...	[more]
A0A6J1E6E2	8.89e-38	97.18	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431022 PE=3 S...	[more]
A0A6J1EBC2	4.81e-36	90.14	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431021 PE=3 S...	[more]

BLAST of Csor.00g010230 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32440.1	1.1e-10	48.44	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	1.1e-10	48.44	ent-kaurenoic acid hydroxylase 2	[more]
AT1G05160.1	2.0e-07	39.06	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR24286:SF290	ENT-KAURENOIC ACID OXIDASE 2-LIKE	coord: 10..71
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 10..71

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g010230.m01	Csor.00g010230.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen