Csor.00g009640 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g009640
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPlant protein of unknown function (DUF247)
LocationCsor_Chr08: 153034 .. 154549 (+)
RNA-Seq ExpressionCsor.00g009640
SyntenyCsor.00g009640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGTGCAAATTTGGGTAGCAGCAATGGAGAAACAGCCACCGCTGTCCATCAAACTTATTAGAGATGACAATGTGGATAAACCTGACGACATGGTTATTCAGGTGATGGAGTGTCTGAATGAACCTGGGTTGGATTCTCCTGCCAACAAACATTCTATCTACAAACTACCATTCTTCATGAGACAAACTCACTCCGAAGCTTTCAACAAAGCTTTCGATCCCCAGGTGGTGTCATTTGGGCCATACCACCATGGGAAGAAACATTTGTCACCCATGGAACGGCAAAAGGCTAAAGCGTTCAACACTCTTAAAACCAGAGGACTGCCCGTTGAAGCCATTGTGAGGGAGGTGTCCACCATTTTGGATGACCTCTTGGAATCATACGATAGGCTTGAGGAAGAATGGACGCAAGATCCCGGGAAGTTCTTGAAGCTCATGATTGTGGATGGGTGTTTCATGCTGCGATTGTTCTGCGACTGCCCGGCATCTCTAGTTAACATGAAGATGGATATAAGGCTGGAAACGCTGCTGCTTGAGAACCAGCTACCCTTGAAGCTTCTGCATAAGCTGCATTCTATAGCAGGCCCTATCTTCATATCAGAAGGCGATGATGAGCGACCACTCCAGGCAACTTAACTTACTTCATCTCCCCCTTCTCCTTCTTCTTCTAAATTCTAATCATCTCTCTACTTCTTCTTCTTATTCTTCCACTTATATACTTAAAAAATGCACACAGGAGCTGATTTGCAATTGGTTGGGCATCTCCCAGAAAGATCTGCTCGATGAATTCTTGCACATTTTGGACATGTATAAGGCCTCATTATTGTATCCTCCGATAGACCGGACGAAGTGGTGTGAGGAGGGGAAGGGCAAGGGCAAGGGGAAGGAGAAGGGGAAGGAGGAGGAGAAGGAGGAGCTTTCAGGCGCGGAGTTTCAAGTCATTCCGCCCGCAACAAAGCTTCATGAAGCGGGGATCAAATTCAAGATGAGCCAGACGAAGAGCCTTAAGGACGTGATTTTCGATGAAGTAGAAGGCGTGTTGAGTCTGCCGATGTTGATGGTGGACGACAACACTGAGTCCACTTTCTTAAACGTGATGGCATTTGAGAAACTCCACGTTGAGGGTGCAGCCCAAGAAGCGCCTTCCCTCATCACGTCTTTCGTGATCTTGATGAGTAATTTGATAGACGACGAGAGAGATGTGGCGCTGCTATCCTCCAAGGGTACGTTGGCAAATGCGCTTGGGAATGACAGACAAGCGGCAGAGTTGTTCAGTCGGCTAGGGAAAGGGGTGGCTATGGGTCCCAACAGGCACATGATGGCGGTGCACAAGATGCTGAATGACTACTGCAAGGAGCCCTGGAATGAACGGTGCGCAACTCTGAAGCACCAGTACTTTCAAAACCCATGGACCGTCATCTCCTTAACCGCCGCTATTTTTGGTTTCCTCATCCTAATCCTCCAGGCCATCTACCAACTTCTTGATTACTACAAGGAGGACAAAAAGAAATAA

mRNA sequence

ATGAAGGTGCAAATTTGGGTAGCAGCAATGGAGAAACAGCCACCGCTGTCCATCAAACTTATTAGAGATGACAATGTGGATAAACCTGACGACATGGTTATTCAGGTGATGGAGTGTCTGAATGAACCTGGGTTGGATTCTCCTGCCAACAAACATTCTATCTACAAACTACCATTCTTCATGAGACAAACTCACTCCGAAGCTTTCAACAAAGCTTTCGATCCCCAGGTGGTGTCATTTGGGCCATACCACCATGGGAAGAAACATTTGTCACCCATGGAACGGCAAAAGGCTAAAGCGTTCAACACTCTTAAAACCAGAGGACTGCCCGTTGAAGCCATTGTGAGGGAGGTGTCCACCATTTTGGATGACCTCTTGGAATCATACGATAGGCTTGAGGAAGAATGGACGCAAGATCCCGGGAAGTTCTTGAAGCTCATGATTGTGGATGGGTGTTTCATGCTGCGATTGTTCTGCGACTGCCCGGCATCTCTAGTTAACATGAAGATGGATATAAGGCTGGAAACGCTGCTGCTTGAGAACCAGCTACCCTTGAAGCTTCTGCATAAGCTGCATTCTATAGCAGGCCCTATCTTCATATCAGAAGGCGATGATGAGCGACCACTCCAGGAGCTGATTTGCAATTGGTTGGGCATCTCCCAGAAAGATCTGCTCGATGAATTCTTGCACATTTTGGACATGTATAAGGCCTCATTATTGTATCCTCCGATAGACCGGACGAAGTGGTGTGAGGAGGGGAAGGGCAAGGGCAAGGGGAAGGAGAAGGGGAAGGAGGAGGAGAAGGAGGAGCTTTCAGGCGCGGAGTTTCAAGTCATTCCGCCCGCAACAAAGCTTCATGAAGCGGGGATCAAATTCAAGATGAGCCAGACGAAGAGCCTTAAGGACGTGATTTTCGATGAAGTAGAAGGCGTGTTGAGTCTGCCGATGTTGATGGTGGACGACAACACTGAGTCCACTTTCTTAAACGTGATGGCATTTGAGAAACTCCACGTTGAGGGTGCAGCCCAAGAAGCGCCTTCCCTCATCACGTCTTTCGTGATCTTGATGAGTAATTTGATAGACGACGAGAGAGATGTGGCGCTGCTATCCTCCAAGGGTACGTTGGCAAATGCGCTTGGGAATGACAGACAAGCGGCAGAGTTGTTCAGTCGGCTAGGGAAAGGGGTGGCTATGGGTCCCAACAGGCACATGATGGCGGTGCACAAGATGCTGAATGACTACTGCAAGGAGCCCTGGAATGAACGGTGCGCAACTCTGAAGCACCAGTACTTTCAAAACCCATGGACCGTCATCTCCTTAACCGCCGCTATTTTTGGTTTCCTCATCCTAATCCTCCAGGCCATCTACCAACTTCTTGATTACTACAAGGAGGACAAAAAGAAATAA

Coding sequence (CDS)

ATGAAGGTGCAAATTTGGGTAGCAGCAATGGAGAAACAGCCACCGCTGTCCATCAAACTTATTAGAGATGACAATGTGGATAAACCTGACGACATGGTTATTCAGGTGATGGAGTGTCTGAATGAACCTGGGTTGGATTCTCCTGCCAACAAACATTCTATCTACAAACTACCATTCTTCATGAGACAAACTCACTCCGAAGCTTTCAACAAAGCTTTCGATCCCCAGGTGGTGTCATTTGGGCCATACCACCATGGGAAGAAACATTTGTCACCCATGGAACGGCAAAAGGCTAAAGCGTTCAACACTCTTAAAACCAGAGGACTGCCCGTTGAAGCCATTGTGAGGGAGGTGTCCACCATTTTGGATGACCTCTTGGAATCATACGATAGGCTTGAGGAAGAATGGACGCAAGATCCCGGGAAGTTCTTGAAGCTCATGATTGTGGATGGGTGTTTCATGCTGCGATTGTTCTGCGACTGCCCGGCATCTCTAGTTAACATGAAGATGGATATAAGGCTGGAAACGCTGCTGCTTGAGAACCAGCTACCCTTGAAGCTTCTGCATAAGCTGCATTCTATAGCAGGCCCTATCTTCATATCAGAAGGCGATGATGAGCGACCACTCCAGGAGCTGATTTGCAATTGGTTGGGCATCTCCCAGAAAGATCTGCTCGATGAATTCTTGCACATTTTGGACATGTATAAGGCCTCATTATTGTATCCTCCGATAGACCGGACGAAGTGGTGTGAGGAGGGGAAGGGCAAGGGCAAGGGGAAGGAGAAGGGGAAGGAGGAGGAGAAGGAGGAGCTTTCAGGCGCGGAGTTTCAAGTCATTCCGCCCGCAACAAAGCTTCATGAAGCGGGGATCAAATTCAAGATGAGCCAGACGAAGAGCCTTAAGGACGTGATTTTCGATGAAGTAGAAGGCGTGTTGAGTCTGCCGATGTTGATGGTGGACGACAACACTGAGTCCACTTTCTTAAACGTGATGGCATTTGAGAAACTCCACGTTGAGGGTGCAGCCCAAGAAGCGCCTTCCCTCATCACGTCTTTCGTGATCTTGATGAGTAATTTGATAGACGACGAGAGAGATGTGGCGCTGCTATCCTCCAAGGGTACGTTGGCAAATGCGCTTGGGAATGACAGACAAGCGGCAGAGTTGTTCAGTCGGCTAGGGAAAGGGGTGGCTATGGGTCCCAACAGGCACATGATGGCGGTGCACAAGATGCTGAATGACTACTGCAAGGAGCCCTGGAATGAACGGTGCGCAACTCTGAAGCACCAGTACTTTCAAAACCCATGGACCGTCATCTCCTTAACCGCCGCTATTTTTGGTTTCCTCATCCTAATCCTCCAGGCCATCTACCAACTTCTTGATTACTACAAGGAGGACAAAAAGAAATAA

Protein sequence

MKVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPANKHSIYKLPFFMRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVSTILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLVNMKMDIRLETLLLENQLPLKLLHKLHSIAGPIFISEGDDERPLQELICNWLGISQKDLLDEFLHILDMYKASLLYPPIDRTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQVIPPATKLHEAGIKFKMSQTKSLKDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLIDDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWNERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYKEDKKK
Homology
BLAST of Csor.00g009640 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 1.2e-29
Identity = 108/419 (25.78%), Postives = 199/419 (47.49%), Query Frame = 0

Query: 71  KAFDPQVVSFGPYHHGKKHLSPMERQKAKA----FNTLKTRGLPVEAIVREVSTILDDLL 130
           KA+ P+VVS GPYH+G+KHL  +++ K +      +  K + +    +V+ V  + D + 
Sbjct: 61  KAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKKKDVEENVLVKAVVDLEDKIR 120

Query: 131 ESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFC----------DCPASLVNMKMDIRLET 190
           +SY     E  +     + +M++DGCF+L +F           D   S+  +   I+ + 
Sbjct: 121 KSY----SEELKTGHDLMFMMVLDGCFILMVFLIMSGNIELSEDPIFSIPWLLSSIQSDL 180

Query: 191 LLLENQLPLKLLHKLHSIAGPIFISEGDD-------ERPLQELICNWLGISQKDLLDEFL 250
           LLLENQ+P  +L  L+ +   I +S   +       + P+ +    W    +K    +  
Sbjct: 181 LLLENQVPFFVLQTLY-VGSKIGVSSDLNRIAFHFFKNPIDKEGSYW----EKHRNYKAK 240

Query: 251 HILDMYKASLLYPPIDRTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQVIPPATKLHEAG 310
           H+LD+ + + L    +  K             +GK      +      +I  A +L   G
Sbjct: 241 HLLDLIRETFLPNTSESDK--ASSPHVQVQLHEGKSGNVPSVDSKAVPLILSAKRLRLQG 300

Query: 311 IKFKMSQTKSLKDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLI 370
           IKF++ ++K    +     +  L +P L  D    S FLN +AFE+ + + + +     I
Sbjct: 301 IKFRLRRSKEDSILNVRLKKNKLQIPQLRFDGFISSFFLNCVAFEQFYTDSSNE-----I 360

Query: 371 TSFVILMSNLIDDERDVALL-SSKGTLANALGNDRQAAELFSRLGKGVAMG-PNRHMMAV 430
           T++++ M  L+++E DV  L + K  + N  G++ + +E F  + K V       ++  V
Sbjct: 361 TTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSEFFKTISKDVVFEVDTSYLNNV 420

Query: 431 HKMLNDYCKEPWNERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYKEDK 467
            K +N+Y K+ +N   A  +H +F++PWT +S  A +F  L+ +LQ+   +L Y  + K
Sbjct: 421 FKGVNEYTKKWYNGLWAGFRHTHFESPWTFLSSCAVLFVILLTMLQSTVAILSYLNDKK 463

BLAST of Csor.00g009640 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.4e-17
Identity = 51/175 (29.14%), Postives = 92/175 (52.57%), Query Frame = 0

Query: 279 IPPATKLHEAGIKFKMSQTKSLKDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHV 338
           IP  + LH+AG++FK +   ++  V FD   G   LP++ +D NTE+   N++A+E  + 
Sbjct: 345 IPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATNT 404

Query: 339 EGAAQEAPSLITSFVILMSNLIDDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAM 398
            G     P + T +  L++ +ID E DV LL  +G L + L +D++AAE+++ + K V +
Sbjct: 405 SG-----PLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGMSKSVRL 464

Query: 399 GPNRHMMAVHKMLNDYCKEPWNERCATLKHQYFQNPWTVISLTAAIFGFLILILQ 454
                +    + +N Y    W  +   L   Y    W +++  AA+   +++ LQ
Sbjct: 465 TKVGFLDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFLAAVLLLMLVSLQ 514

BLAST of Csor.00g009640 vs. NCBI nr
Match: KAG6592830.1 (UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 939 bits (2426), Expect = 0.0
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 0

Query: 1   MKVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPANKHSIYKLPFF 60
           MKVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPANKHSIYKLPFF
Sbjct: 1   MKVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPANKHSIYKLPFF 60

Query: 61  MRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVST 120
           MRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVST
Sbjct: 61  MRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVST 120

Query: 121 ILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLVNMKMDIRLETLLLE 180
           ILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLVNMKMDIRLETLLLE
Sbjct: 121 ILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLVNMKMDIRLETLLLE 180

Query: 181 NQLPLKLLHKLHSIAGPIFISEGDDERPLQELICNWLGISQKDLLDEFLHILDMYKASLL 240
           NQLPLKLLHKLHSIAGPIFISEGDDERPLQELICNWLGISQKDLLDEFLHILDMYKASLL
Sbjct: 181 NQLPLKLLHKLHSIAGPIFISEGDDERPLQELICNWLGISQKDLLDEFLHILDMYKASLL 240

Query: 241 YPPIDRTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQVIPPATKLHEAGIKFKMSQTKSL 300
           YPPIDRTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQVIPPATKLHEAGIKFKMSQTKSL
Sbjct: 241 YPPIDRTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQVIPPATKLHEAGIKFKMSQTKSL 300

Query: 301 KDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLI 360
           KDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLI
Sbjct: 301 KDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLI 360

Query: 361 DDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWN 420
           DDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWN
Sbjct: 361 DDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWN 420

Query: 421 ERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYKEDKKK 468
           ERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYKEDKKK
Sbjct: 421 ERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYKEDKKK 468

BLAST of Csor.00g009640 vs. NCBI nr
Match: XP_022960231.1 (UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022960232.1 UPF0481 protein At3g47200-like [Cucurbita moschata])

HSP 1 Score: 890 bits (2299), Expect = 0.0
Identity = 445/468 (95.09%), Postives = 450/468 (96.15%), Query Frame = 0

Query: 1   MKVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPANKHSIYKLPFF 60
           MKVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSP NKHSIYKLPFF
Sbjct: 1   MKVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPTNKHSIYKLPFF 60

Query: 61  MRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVST 120
           MRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVST
Sbjct: 61  MRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVST 120

Query: 121 ILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLVNMKMDIRLETLLLE 180
           ILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASL+NMKMDIRLETLLLE
Sbjct: 121 ILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLINMKMDIRLETLLLE 180

Query: 181 NQLPLKLLHKLHSIAGPIFISEGDDERPLQELICNWLGISQKDLLDEFLHILDMYKASLL 240
           NQLPLKLLHKLHSIAGPIFISEGDDERPLQELICNWLGISQKDLLDEFLHILD+YKASLL
Sbjct: 181 NQLPLKLLHKLHSIAGPIFISEGDDERPLQELICNWLGISQKDLLDEFLHILDLYKASLL 240

Query: 241 YPPIDRTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQVIPPATKLHEAGIKFKMSQTKSL 300
            PPIDRTKWCE+G                ELSGAEFQVIPPATKLHEAGIKFKMSQTKSL
Sbjct: 241 CPPIDRTKWCEKG----------------ELSGAEFQVIPPATKLHEAGIKFKMSQTKSL 300

Query: 301 KDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLI 360
           KDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLI
Sbjct: 301 KDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLI 360

Query: 361 DDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWN 420
           DDERDVALLSSKGTLANALGNDR+AAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWN
Sbjct: 361 DDERDVALLSSKGTLANALGNDREAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWN 420

Query: 421 ERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYKEDKKK 468
           ERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYK+DKKK
Sbjct: 421 ERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYKKDKKK 452

BLAST of Csor.00g009640 vs. NCBI nr
Match: XP_023513985.1 (UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 783 bits (2022), Expect = 2.14e-283
Identity = 395/468 (84.40%), Postives = 420/468 (89.74%), Query Frame = 0

Query: 1   MKVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPANKHSIYKLPFF 60
           MKVQIWVAAMEKQPPLSIKLIRDDNVDKPD MVIQVM CLNEPGLDSPA  H IYKLPFF
Sbjct: 1   MKVQIWVAAMEKQPPLSIKLIRDDNVDKPD-MVIQVMGCLNEPGLDSPAITHCIYKLPFF 60

Query: 61  MRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVST 120
           MRQTH EAFN AFDPQVVSFGPYH GK HLSPMER+KAK FN+LKTRGLPVEA+V  V +
Sbjct: 61  MRQTHPEAFNTAFDPQVVSFGPYHQGKHHLSPMEREKAKVFNSLKTRGLPVEAVVSGVHS 120

Query: 121 ILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLVNMKMDIRLETLLLE 180
           ILDDLL SYDRL++EWTQ PGKFL LMIVDGCF+L L C+CPASL+NMKMDIR ETLLLE
Sbjct: 121 ILDDLLGSYDRLDQEWTQHPGKFLMLMIVDGCFLLELLCNCPASLINMKMDIRQETLLLE 180

Query: 181 NQLPLKLLHKLHSIAGPIFISEGDDERPLQELICNWLGISQKDLLDEFLHILDMYKASLL 240
           NQLP+KLLH+L+SIAGPIFIS+ D E+  +ELICNWLGIS  DL +EFLHILDMYKASLL
Sbjct: 181 NQLPMKLLHQLYSIAGPIFISQRDKEQQPKELICNWLGISTTDLDEEFLHILDMYKASLL 240

Query: 241 YPPIDRTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQVIPPATKLHEAGIKFKMSQTKSL 300
            PP+DRT WCE                K++LSGAE QVIPPATKL EAGI+FK S+TKSL
Sbjct: 241 SPPVDRTPWCE----------------KDKLSGAECQVIPPATKLREAGIRFKRSRTKSL 300

Query: 301 KDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLI 360
           KDVIFD+ +GVLSLPMLMVDD+TESTFLNVMAFEKLHVEGAAQEAPSL+TSFVILMSNLI
Sbjct: 301 KDVIFDKEQGVLSLPMLMVDDDTESTFLNVMAFEKLHVEGAAQEAPSLVTSFVILMSNLI 360

Query: 361 DDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWN 420
           DDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWN
Sbjct: 361 DDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWN 420

Query: 421 ERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYKEDKKK 468
           ERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYKEDKKK
Sbjct: 421 ERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYKEDKKK 451

BLAST of Csor.00g009640 vs. NCBI nr
Match: KAG7025238.1 (UPF0481 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 740 bits (1911), Expect = 3.03e-261
Identity = 379/436 (86.93%), Postives = 380/436 (87.16%), Query Frame = 0

Query: 1   MKVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPANKHSIYKLPFF 60
           MKVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNE GLDSPANKHSIYKLPFF
Sbjct: 1   MKVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNELGLDSPANKHSIYKLPFF 60

Query: 61  MRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVST 120
           MRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVR VST
Sbjct: 61  MRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVRGVST 120

Query: 121 ILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLVNMKMDIRLETLLLE 180
           ILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASL               
Sbjct: 121 ILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASL--------------- 180

Query: 181 NQLPLKLLHKLHSIAGPIFISEGDDERPLQELICNWLGISQKDLLDEFLHILDMYKASLL 240
                                         ELICNWLGISQK LLDEFLHILDMYKASLL
Sbjct: 181 ------------------------------ELICNWLGISQKHLLDEFLHILDMYKASLL 240

Query: 241 YPPIDRTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQVIPPATKLHEAGIKFKMSQTKSL 300
            PPIDRTKWCEEGKGKGK      E+EKEELSGAEFQVIPPATKLHE GIKFKMSQTKSL
Sbjct: 241 CPPIDRTKWCEEGKGKGK------EKEKEELSGAEFQVIPPATKLHEVGIKFKMSQTKSL 300

Query: 301 KDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLI 360
           KDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLI
Sbjct: 301 KDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLI 360

Query: 361 DDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWN 420
           DDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWN
Sbjct: 361 DDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWN 385

Query: 421 ERCATLKHQYFQNPWT 436
           ERCATLKHQYFQNPWT
Sbjct: 421 ERCATLKHQYFQNPWT 385

BLAST of Csor.00g009640 vs. NCBI nr
Match: XP_023004238.1 (UPF0481 protein At3g47200-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 523 bits (1347), Expect = 6.11e-181
Identity = 282/465 (60.65%), Postives = 341/465 (73.33%), Query Frame = 0

Query: 10  MEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPANKH----------SIYKLPF 69
           MEKQPPL + + RDDN+    DMV++V   L++  LDSPA  +          SIYK+PF
Sbjct: 1   MEKQPPLQVMVGRDDNIGDKADMVVRVKGTLDQL-LDSPAILNMEAEQSSELLSIYKIPF 60

Query: 70  FMRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGL-PVEAIVREV 129
           FM QTH     KA++PQVVS GPY+HGK+HLSPME +K K F++ KTR L  VE+IVR V
Sbjct: 61  FMTQTHP----KAYEPQVVSLGPYNHGKQHLSPMELEKLKLFHSFKTRCLLDVESIVRGV 120

Query: 130 STILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLVNMKMDIRLETLL 189
           STILD+L+ESYDRLEEEWTQDPGKFL+LMIVDGCFML     CP SL+N+  DI+ + LL
Sbjct: 121 STILDELMESYDRLEEEWTQDPGKFLQLMIVDGCFMLGFLISCPNSLINVSPDIKQDMLL 180

Query: 190 LENQLPLKLLHKLHSIAGP-IFISEGDDERPLQELICNWLGISQKDLLDEFLHILDMYKA 249
           LENQLP+ LL KL+SIAG  + + + D ++    L+C WL I Q +++ + LHIL+MYK 
Sbjct: 181 LENQLPMLLLEKLYSIAGRNVQLPQQDPKK----LVCKWLSIPQNEVMKDCLHILEMYKE 240

Query: 250 SLLYPPIDRTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQVIPPATKLHEAGIKFKMSQT 309
           SLLYPPIDR  W  E                 + S  E QVIPPATKLHEAGIKFK S+T
Sbjct: 241 SLLYPPIDRRDWSAEL----------------DHSDPECQVIPPATKLHEAGIKFKRSKT 300

Query: 310 KSLKDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMS 369
           +SL+DV FD   GVL LP LMVDD+TEST LNVMAFEKLH++   +     +TSFVILMS
Sbjct: 301 ESLRDVWFDTKRGVLWLPQLMVDDDTESTMLNVMAFEKLHMKAGRK-----VTSFVILMS 360

Query: 370 NLIDDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKE 429
           NLIDDERDVA+L+ +  LANA+GND++AA LFSRLG G AMG + HM  VHKM+N +C +
Sbjct: 361 NLIDDERDVAVLAGEKILANAVGNDKEAAGLFSRLGSGAAMGLDSHMAGVHKMVNAHCNQ 420

Query: 430 PWNERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYY 462
           PWNERCATLKH YFQ+PWT+ISL AAIFGF+ILILQAIYQ LDYY
Sbjct: 421 PWNERCATLKHDYFQSPWTIISLCAAIFGFIILILQAIYQFLDYY 435

BLAST of Csor.00g009640 vs. ExPASy TrEMBL
Match: A0A6J1H6V9 (UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111461034 PE=4 SV=1)

HSP 1 Score: 890 bits (2299), Expect = 0.0
Identity = 445/468 (95.09%), Postives = 450/468 (96.15%), Query Frame = 0

Query: 1   MKVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPANKHSIYKLPFF 60
           MKVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSP NKHSIYKLPFF
Sbjct: 1   MKVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPTNKHSIYKLPFF 60

Query: 61  MRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVST 120
           MRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVST
Sbjct: 61  MRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVST 120

Query: 121 ILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLVNMKMDIRLETLLLE 180
           ILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASL+NMKMDIRLETLLLE
Sbjct: 121 ILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLINMKMDIRLETLLLE 180

Query: 181 NQLPLKLLHKLHSIAGPIFISEGDDERPLQELICNWLGISQKDLLDEFLHILDMYKASLL 240
           NQLPLKLLHKLHSIAGPIFISEGDDERPLQELICNWLGISQKDLLDEFLHILD+YKASLL
Sbjct: 181 NQLPLKLLHKLHSIAGPIFISEGDDERPLQELICNWLGISQKDLLDEFLHILDLYKASLL 240

Query: 241 YPPIDRTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQVIPPATKLHEAGIKFKMSQTKSL 300
            PPIDRTKWCE+G                ELSGAEFQVIPPATKLHEAGIKFKMSQTKSL
Sbjct: 241 CPPIDRTKWCEKG----------------ELSGAEFQVIPPATKLHEAGIKFKMSQTKSL 300

Query: 301 KDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLI 360
           KDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLI
Sbjct: 301 KDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLI 360

Query: 361 DDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWN 420
           DDERDVALLSSKGTLANALGNDR+AAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWN
Sbjct: 361 DDERDVALLSSKGTLANALGNDREAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEPWN 420

Query: 421 ERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYKEDKKK 468
           ERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYK+DKKK
Sbjct: 421 ERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYKKDKKK 452

BLAST of Csor.00g009640 vs. ExPASy TrEMBL
Match: A0A6J1KYV8 (UPF0481 protein At3g47200-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497633 PE=4 SV=1)

HSP 1 Score: 523 bits (1347), Expect = 2.96e-181
Identity = 282/465 (60.65%), Postives = 341/465 (73.33%), Query Frame = 0

Query: 10  MEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPANKH----------SIYKLPF 69
           MEKQPPL + + RDDN+    DMV++V   L++  LDSPA  +          SIYK+PF
Sbjct: 1   MEKQPPLQVMVGRDDNIGDKADMVVRVKGTLDQL-LDSPAILNMEAEQSSELLSIYKIPF 60

Query: 70  FMRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGL-PVEAIVREV 129
           FM QTH     KA++PQVVS GPY+HGK+HLSPME +K K F++ KTR L  VE+IVR V
Sbjct: 61  FMTQTHP----KAYEPQVVSLGPYNHGKQHLSPMELEKLKLFHSFKTRCLLDVESIVRGV 120

Query: 130 STILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLVNMKMDIRLETLL 189
           STILD+L+ESYDRLEEEWTQDPGKFL+LMIVDGCFML     CP SL+N+  DI+ + LL
Sbjct: 121 STILDELMESYDRLEEEWTQDPGKFLQLMIVDGCFMLGFLISCPNSLINVSPDIKQDMLL 180

Query: 190 LENQLPLKLLHKLHSIAGP-IFISEGDDERPLQELICNWLGISQKDLLDEFLHILDMYKA 249
           LENQLP+ LL KL+SIAG  + + + D ++    L+C WL I Q +++ + LHIL+MYK 
Sbjct: 181 LENQLPMLLLEKLYSIAGRNVQLPQQDPKK----LVCKWLSIPQNEVMKDCLHILEMYKE 240

Query: 250 SLLYPPIDRTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQVIPPATKLHEAGIKFKMSQT 309
           SLLYPPIDR  W  E                 + S  E QVIPPATKLHEAGIKFK S+T
Sbjct: 241 SLLYPPIDRRDWSAEL----------------DHSDPECQVIPPATKLHEAGIKFKRSKT 300

Query: 310 KSLKDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMS 369
           +SL+DV FD   GVL LP LMVDD+TEST LNVMAFEKLH++   +     +TSFVILMS
Sbjct: 301 ESLRDVWFDTKRGVLWLPQLMVDDDTESTMLNVMAFEKLHMKAGRK-----VTSFVILMS 360

Query: 370 NLIDDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKE 429
           NLIDDERDVA+L+ +  LANA+GND++AA LFSRLG G AMG + HM  VHKM+N +C +
Sbjct: 361 NLIDDERDVAVLAGEKILANAVGNDKEAAGLFSRLGSGAAMGLDSHMAGVHKMVNAHCNQ 420

Query: 430 PWNERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYY 462
           PWNERCATLKH YFQ+PWT+ISL AAIFGF+ILILQAIYQ LDYY
Sbjct: 421 PWNERCATLKHDYFQSPWTIISLCAAIFGFIILILQAIYQFLDYY 435

BLAST of Csor.00g009640 vs. ExPASy TrEMBL
Match: A0A6J1KVQ6 (UPF0481 protein At3g47200-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111497633 PE=4 SV=1)

HSP 1 Score: 523 bits (1346), Expect = 4.05e-181
Identity = 282/464 (60.78%), Postives = 338/464 (72.84%), Query Frame = 0

Query: 10  MEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPANKH----------SIYKLPF 69
           MEKQPPL + + RDDN+    DMV++V   L++  LDSPA  +          SIYK+PF
Sbjct: 1   MEKQPPLQVMVGRDDNIGDKADMVVRVKGTLDQL-LDSPAILNMEAEQSSELLSIYKIPF 60

Query: 70  FMRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGL-PVEAIVREV 129
           FM QTH     KA++PQVVS GPY+HGK+HLSPME +K K F++ KTR L  VE+IVR V
Sbjct: 61  FMTQTHP----KAYEPQVVSLGPYNHGKQHLSPMELEKLKLFHSFKTRCLLDVESIVRGV 120

Query: 130 STILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLVNMKMDIRLETLL 189
           STILD+L+ESYDRLEEEWTQDPGKFL+LMIVDGCFML     CP SL+N+  DI+ + LL
Sbjct: 121 STILDELMESYDRLEEEWTQDPGKFLQLMIVDGCFMLGFLISCPNSLINVSPDIKQDMLL 180

Query: 190 LENQLPLKLLHKLHSIAGPIFISEGDDERPLQELICNWLGISQKDLLDEFLHILDMYKAS 249
           LENQLP+ LL KL+SIAG       D ++    L+C WL I Q +++ + LHIL+MYK S
Sbjct: 181 LENQLPMLLLEKLYSIAGRNVQLPQDPKK----LVCKWLSIPQNEVMKDCLHILEMYKES 240

Query: 250 LLYPPIDRTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQVIPPATKLHEAGIKFKMSQTK 309
           LLYPPIDR  W  E                 + S  E QVIPPATKLHEAGIKFK S+T+
Sbjct: 241 LLYPPIDRRDWSAEL----------------DHSDPECQVIPPATKLHEAGIKFKRSKTE 300

Query: 310 SLKDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSN 369
           SL+DV FD   GVL LP LMVDD+TEST LNVMAFEKLH++   +     +TSFVILMSN
Sbjct: 301 SLRDVWFDTKRGVLWLPQLMVDDDTESTMLNVMAFEKLHMKAGRK-----VTSFVILMSN 360

Query: 370 LIDDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEP 429
           LIDDERDVA+L+ +  LANA+GND++AA LFSRLG G AMG + HM  VHKM+N +C +P
Sbjct: 361 LIDDERDVAVLAGEKILANAVGNDKEAAGLFSRLGSGAAMGLDSHMAGVHKMVNAHCNQP 420

Query: 430 WNERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYY 462
           WNERCATLKH YFQ+PWT+ISL AAIFGF+ILILQAIYQ LDYY
Sbjct: 421 WNERCATLKHDYFQSPWTIISLCAAIFGFIILILQAIYQFLDYY 434

BLAST of Csor.00g009640 vs. ExPASy TrEMBL
Match: A0A6J1HB25 (UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111461183 PE=4 SV=1)

HSP 1 Score: 501 bits (1289), Expect = 1.87e-172
Identity = 273/467 (58.46%), Postives = 334/467 (71.52%), Query Frame = 0

Query: 10  MEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPANKH----------SIYKLPF 69
           MEK P L + L RDDN+    DMV++V   L++  LDSPA  +          SIYK+PF
Sbjct: 1   MEKHPSLQVMLGRDDNIGDNADMVVRVKGTLDQL-LDSPAILNMEAEQSSELLSIYKIPF 60

Query: 70  FMRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGL-PVEAIVREV 129
           FM QTH     KA++PQVVS GPY+HGK+HLSPME +K K F++ K R L  VE+IVR V
Sbjct: 61  FMTQTHP----KAYEPQVVSLGPYNHGKQHLSPMELEKLKLFHSFKARCLLDVESIVRGV 120

Query: 130 STILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLVNMKMDIRLETLL 189
           STILD+L+ESYD+LEE+W +DPGKFL+LMIVDGCFML    +CP SL+N+  DI+ + LL
Sbjct: 121 STILDELMESYDKLEEDWKEDPGKFLQLMIVDGCFMLGFLINCPDSLINVSPDIKQDMLL 180

Query: 190 LENQLPLKLLHKLHSIAGPIFISEGDDERPLQELICNWLGISQKDLLDEFLHILDMYKAS 249
           LENQLP+ LL KL+SIA         D   L++L+ NWL I + +++ + LHIL+MYK S
Sbjct: 181 LENQLPMLLLEKLYSIADRNVQLPQQD---LKKLVSNWLNIPRNEVMKDCLHILEMYKES 240

Query: 250 LLYPPIDRTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQVIPPATKLHEAGIKFKMSQTK 309
           LL+PPIDRT W  E                   S  E QVIPPATKL EAGIKFK S+T 
Sbjct: 241 LLHPPIDRTDWSVELGH----------------SDPECQVIPPATKLREAGIKFKRSKTG 300

Query: 310 SLKDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSN 369
           SL DV FD   GVL LP LMVDDNTEST LNVMAFEKLH++   +     +TSFVILMSN
Sbjct: 301 SLTDVFFDAKGGVLWLPRLMVDDNTESTLLNVMAFEKLHMKAGRK-----VTSFVILMSN 360

Query: 370 LIDDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGPNRHMMAVHKMLNDYCKEP 429
           LIDDERDVA+L+ +  LANA+GND++AA LF+RLG G AMG + HM  VHK +N++C +P
Sbjct: 361 LIDDERDVAVLAGEQVLANAVGNDKEAAGLFNRLGSGAAMGLDTHMAWVHKKVNEHCNQP 420

Query: 430 WNERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYKED 465
           WNERCATLKH+YFQ+PWT+ISL AAIFGF+ILILQAIYQ LDYY + 
Sbjct: 421 WNERCATLKHEYFQSPWTIISLCAAIFGFIILILQAIYQFLDYYLKS 438

BLAST of Csor.00g009640 vs. ExPASy TrEMBL
Match: A0A6J1KTE2 (uncharacterized protein LOC111498069 OS=Cucurbita maxima OX=3661 GN=LOC111498069 PE=4 SV=1)

HSP 1 Score: 449 bits (1155), Expect = 8.48e-154
Identity = 238/360 (66.11%), Postives = 268/360 (74.44%), Query Frame = 0

Query: 1   MKVQIWVAAMEKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPANKHSIYKLPFF 60
           MKVQIWVAAMEKQPPLSIK+IRDDNVD+PDDMVIQVMECLNEPGLDSPA KHSIYKLPFF
Sbjct: 1   MKVQIWVAAMEKQPPLSIKIIRDDNVDQPDDMVIQVMECLNEPGLDSPAIKHSIYKLPFF 60

Query: 61  MRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEAIVREVST 120
           MR+THSEAFN+AFDPQVVSFGPYHHGK+HLSPMER+KAK F++LKT GLPVEAIV  VST
Sbjct: 61  MRRTHSEAFNQAFDPQVVSFGPYHHGKQHLSPMEREKAKVFDSLKTGGLPVEAIVSCVST 120

Query: 121 ILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASLVNMKMDIRLETLLLE 180
           IL DLLESYDRLEEEWTQ PGKFLKLMIVDGCFML+LFC+CP SL NMKMDIRL+TLLLE
Sbjct: 121 ILYDLLESYDRLEEEWTQHPGKFLKLMIVDGCFMLQLFCNCPTSLSNMKMDIRLDTLLLE 180

Query: 181 NQLPLKLLHKLHSIAGPIFISEGDDER-----------------------PLQELICNWL 240
           NQLPLKLLHKL+SIAGPIFIS+                             L+ ++CNWL
Sbjct: 181 NQLPLKLLHKLYSIAGPIFISQQPKATYFISPFFFFFFFFFHLYTSKCTLELKSVVCNWL 240

Query: 241 GISQKDLLDEFLHILDMYKASLLYPPIDRTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQ 300
           GIS  +L+ EFLH+LDMY+ASL+   +D T W                 +K+ELSGAE Q
Sbjct: 241 GISTNNLVQEFLHVLDMYRASLVCTHVDWTNW----------------NQKKELSGAECQ 300

Query: 301 VIPPATKLHEAGIKFKMSQTKSLKDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLH 337
           VIPPAT+L EAG++FK S T SL DV +D+                     NVMAFEKL+
Sbjct: 301 VIPPATELREAGMRFKTSGTTSLGDVKYDKK--------------------NVMAFEKLN 324

BLAST of Csor.00g009640 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 196.1 bits (497), Expect = 6.5e-50
Identity = 145/499 (29.06%), Postives = 240/499 (48.10%), Query Frame = 0

Query: 11  EKQPPLSIKLIRDDNVDKPDDMVIQVMECLNEPGLDSPA---NKHSIYKLPFFMRQTHSE 70
           + Q   +I +I++   D  DD VI + + L +   D       K  IY++P+++++    
Sbjct: 59  QTQSDPAIYVIKESPKDSRDDWVISITDKLEQAHRDDDTTLWGKLCIYRVPYYLQEND-- 118

Query: 71  AFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFN-TLKTRGLPVEAIVREVSTILDDLL 130
             NK++ PQ VS GPYHHGKK L  M+R K +A N  LK     ++  +  +  + +   
Sbjct: 119 --NKSYFPQTVSLGPYHHGKKRLRSMDRHKWRAVNRVLKRTNQGIKMYIDAMRELEEKAR 178

Query: 131 ESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLF--------------CDCPASLVNMKMDI 190
             Y   E   +    +F++++++DGCF+L LF               D   ++      I
Sbjct: 179 ACY---EGPLSLSSNEFIEMLVLDGCFVLELFRGAVEGFTELGYARNDPVFAMRGSMHSI 238

Query: 191 RLETLLLENQLPLKLLHKL----------HSIAGPIFISEGDDERPLQELICN------W 250
           + + ++LENQLPL +L++L            +   + I   D   P  E +         
Sbjct: 239 QRDMVMLENQLPLFVLNRLLELQLGTRNQTGLVAQLAIRFFDPLMPTDEPLTKSGQSKLE 298

Query: 251 LGISQKDLLDEF-----LHILDMYKASLL------YPPIDRTKWCEEGKGKGKGKEKGKE 310
             +++    D F     LH LD+++ SLL       P + R +W    +   K ++    
Sbjct: 299 NSLARDKSFDPFADMGELHCLDVFRRSLLRSSPKPEPRLTRKRWSRNTRVADKRRQ---- 358

Query: 311 EEKEELSGAEFQVIPPATKLHEAGIKFKMSQTKSLKDVIFDEVEGVLSLPMLMVDDNTES 370
                      Q+I   T+L EAGIKF+  +T    D+ F    G L +P L++ D T+S
Sbjct: 359 -----------QLIHCVTELKEAGIKFRRRKTDRFWDMQFK--NGYLEIPRLLIHDGTKS 418

Query: 371 TFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLIDDERDVALLSSKGTLANALGNDRQA 430
            FLN++AFE+ H++ +       ITS++I M NLID   DV+ L   G + + LG+D + 
Sbjct: 419 LFLNLIAFEQCHIDSSND-----ITSYIIFMDNLIDSHEDVSYLHYCGIIEHWLGSDSEV 478

Query: 431 AELFSRLGKGVAMG-PNRHMMAVHKMLNDYCKEPWNERCATLKHQYFQNPWTVISLTAAI 464
           A+LF+RL + V     + ++  +   +N Y    WN   ATLKH+YF NPW ++S  AA+
Sbjct: 479 ADLFNRLCQEVVFDTEDSYLSRLSIEVNRYYDHKWNAWRATLKHKYFNNPWAIVSFCAAV 528

BLAST of Csor.00g009640 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 196.1 bits (497), Expect = 6.5e-50
Identity = 144/477 (30.19%), Postives = 229/477 (48.01%), Query Frame = 0

Query: 30  DDMVIQVMECLNEPGLDSPA---NKHSIYKLPFFMRQTHSEAFNKAFDPQVVSFGPYHHG 89
           D  VI + + L +   D       K  IY++P ++++       K++ PQ VS GPYHHG
Sbjct: 88  DSWVISIRDKLEQADRDDDTTIWGKLCIYRVPHYLQEND----KKSYFPQTVSLGPYHHG 147

Query: 90  KKHLSPMERQKAKAFN-TLKTRGLPVEAIVREVSTILDDLLESYDRLEEEWTQDPGKFLK 149
           KK L PMER K +A N  LK     +E     +  + +     Y   E   +    +F +
Sbjct: 148 KKRLRPMERHKWRALNKVLKRLKQRIEMYTNAMRELEEKARACY---EGPISLSRNEFTE 207

Query: 150 LMIVDGCFMLRLF--------------CDCPASLVNMKMDIRLETLLLENQLPLKLLHKL 209
           ++++DGCF+L LF               D   ++  +   I+ + ++LENQLPL +L +L
Sbjct: 208 MLVLDGCFVLELFRGTVEGFTEIGYARNDPVFAMRGLMHSIQRDMIMLENQLPLFVLDRL 267

Query: 210 HSIA------------------GPIFISEGDDERPLQELICNWLGISQKDLLDE-FLHIL 269
             +                    P+  +     +P Q  + NWL  S   L D+  LH L
Sbjct: 268 LELQLGTQNQTGIVAHVAVKFFDPLMPTGEALTKPDQSKLMNWLEKSLDTLGDKGELHCL 327

Query: 270 DMYKASLLY-PPIDRTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQVIPPATKLHEAGIK 329
           D+++ SLL   P   T          +   K        +   + Q++   T+L EAG+K
Sbjct: 328 DVFRRSLLQSSPTPNT----------RSLLKRLTRNTRVVDKRQQQLVHCVTELREAGVK 387

Query: 330 FKMSQTKSLKDVIFDEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITS 389
           F+  +T    D+ F    G L +P L++ D T+S F N++AFE+ H+E +     + ITS
Sbjct: 388 FRKRKTDRFWDIEFK--NGYLEIPKLLIHDGTKSLFSNLIAFEQCHIESS-----NHITS 447

Query: 390 FVILMSNLIDDERDVALLSSKGTLANALGNDRQAAELFSRLGKGVAMGP-NRHMMAVHKM 449
           ++I M NLI+   DV+ L   G + + LG+D + A+LF+RL + V   P + H+  +   
Sbjct: 448 YIIFMDNLINSSEDVSYLHYCGIIEHWLGSDSEVADLFNRLCQEVVFDPKDSHLSRLSGD 507

Query: 450 LNDYCKEPWNERCATLKHQYFQNPWTVISLTAAIFGFLILILQAIYQLLDYYKEDKK 468
           +N Y    WN   ATL H+YF NPW   S +AA+   L+ + Q+ Y +  YYK + K
Sbjct: 508 VNRYYNRKWNVLKATLTHKYFNNPWAYFSFSAAVILLLLTLCQSFYAVYAYYKPNSK 540

BLAST of Csor.00g009640 vs. TAIR 10
Match: AT3G50130.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 180.6 bits (457), Expect = 2.8e-45
Identity = 134/458 (29.26%), Postives = 217/458 (47.38%), Query Frame = 0

Query: 50  NKHSIYKLPFFMRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGL 109
           +K  IY++P ++++ +     K++ PQ VS GP+HHG KHL PM+R K +A N +  R  
Sbjct: 137 DKLCIYRVPQYLQENN----KKSYFPQTVSLGPFHHGNKHLLPMDRHKWRAVNMVMAR-- 196

Query: 110 PVEAIVREVSTILDDLLESYDR----LEEEWTQDPGKFLKLMIVDGCFMLRLFCDCPASL 169
                  ++   +D + E  DR     E        KF +++++DGCF+L LF       
Sbjct: 197 ----TKHDIEMYIDAMKELEDRARACYEGPIDLSSNKFSEMLVLDGCFVLELFRGADEGF 256

Query: 170 VNMKMD--------------IRLETLLLENQLPLKLLHKLHSI----------AGPIFIS 229
             +  D              I+ + ++LENQLPL +L++L  I             + + 
Sbjct: 257 SELGYDRNDPVFAMRGSMHSIQRDMVMLENQLPLFVLNRLLEIQLGKRHQTGLVSRLAVR 316

Query: 230 EGDDERPLQELICN----------WLGISQKDLLDEFLHILDMYKASLL------YPPID 289
             D   P  E +            +  I+ KD  +  LH LD+++ +LL       P + 
Sbjct: 317 FFDPLMPTDEPLTKTDDSLEQDKFFNPIADKDKGE--LHCLDVFRRNLLRPCSNPEPRLS 376

Query: 290 RTKWCEEGKGKGKGKEKGKEEEKEELSGAEFQVIPPATKLHEAGIKFKMSQTKSLKDVIF 349
           R +W    +   K ++               Q+I   T+L EAGIKF+  +T    D+ F
Sbjct: 377 RMRWSWRTRVADKRQQ---------------QLIHCVTELREAGIKFRTRKTDRFWDIRF 436

Query: 350 DEVEGVLSLPMLMVDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLIDDERD 409
               G L +P L++ D T+S F N++AFE+ H++ +       ITS++I M NLID   D
Sbjct: 437 K--NGYLEIPKLLIHDGTKSLFSNLIAFEQCHIDSSND-----ITSYIIFMDNLIDSSED 496

Query: 410 VALLSSKGTLANALGNDRQAAELFSRLGKGVAMGP-NRHMMAVHKMLNDYCKEPWNERCA 463
           V  L   G + + LGND + A+LF+RL + VA  P N ++  +   ++      WN   A
Sbjct: 497 VRYLHYCGIIEHWLGNDYEVADLFNRLCQEVAFDPQNSYLSQLSNKVDRNYSRKWNVLKA 556

BLAST of Csor.00g009640 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 179.5 bits (454), Expect = 6.3e-45
Identity = 124/437 (28.38%), Postives = 219/437 (50.11%), Query Frame = 0

Query: 54  IYKLPFFMRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGLPVEA 113
           IY++P ++++  +    K++ PQ+VS GPYHHG KHL PMER K +A N +  R      
Sbjct: 106 IYRVPPYLQENDT----KSYMPQIVSIGPYHHGHKHLMPMERHKWRAVNMVMARA----- 165

Query: 114 IVREVSTILDDLLESYDRLEEEW----TQDPGKFLKLMIVDGCFMLRLF----------- 173
              ++   +D + E  ++    +      +  +F++++++DG F++ +F           
Sbjct: 166 -KHDIEMYIDAMKELEEKARACYQGPINMNRNEFIEMLVLDGVFIIEIFKGTSEGFQEIG 225

Query: 174 ---CDCPASLVNMKMDIRLETLLLENQLPLKLLHKLHSIAGPIFISEGDDE--RPLQELI 233
               D    +  +   IR + ++LENQLP  +L  L  +  P  + + + +  +P  + +
Sbjct: 226 YAPNDPVFGMRGLMQSIRRDMVMLENQLPWSVLKGLLQLQRPDVLDKVNVQLFQPFFQPL 285

Query: 234 CNWLGISQKDLLDE--FLHILDMYKASLLYPPIDRTKWCEEGKGKGKGKEKGKEEEKEEL 293
                +  +++L E   LH LD+ +  LL           +  G         +E+   +
Sbjct: 286 -----LPTREVLTEEGGLHCLDVLRRGLL-----------QSSGT-------SDEDMSMV 345

Query: 294 SGAEFQVIPPATKLHEAGIKFKMSQTKSLKDVIFDEVEGVLSLPMLMVDDNTESTFLNVM 353
           +    Q+I   T+L  AG++F   +T    D+ F    G L +P L++ D T+S FLN++
Sbjct: 346 NKQPQQLIHCVTELRNAGVEFMRKETGHFWDIEFK--NGYLKIPKLLIHDGTKSLFLNLI 405

Query: 354 AFEKLHVEGAAQEAPSLITSFVILMSNLIDDERDVALLSSKGTLANALGNDRQAAELFSR 413
           AFE+ H++ + +     ITS++I M NLI+   DV+ L   G + N LG+D + ++LF+ 
Sbjct: 406 AFEQCHIKSSKK-----ITSYIIFMDNLINSSEDVSYLHHYGIIENWLGSDSEVSDLFNG 465

Query: 414 LGKGVAMGPN-RHMMAVHKMLNDYCKEPWNERCATLKHQYFQNPWTVISLTAAIFGFLIL 468
           LGK V   PN  ++ A+   +N Y +  WN   ATL+H+YF NPW   S  AA+   +  
Sbjct: 466 LGKEVIFDPNDGYLSALTGEVNIYYRRKWNYLKATLRHKYFNNPWAYFSFIAAVTLLIFT 502

BLAST of Csor.00g009640 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 178.3 bits (451), Expect = 1.4e-44
Identity = 130/446 (29.15%), Postives = 219/446 (49.10%), Query Frame = 0

Query: 50  NKHSIYKLPFFMRQTHSEAFNKAFDPQVVSFGPYHHGKKHLSPMERQKAKAFNTLKTRGL 109
           +K  IY++PF++++       K++ PQ VS GPYHHGK HL PMER K +A N +  R  
Sbjct: 88  DKLCIYRVPFYLQEND----KKSYLPQTVSIGPYHHGKVHLRPMERHKWRAVNMIMARTK 147

Query: 110 -PVEAIVREVSTILDDLLESYDRLEEEWTQDPGKFLKLMIVDGCFMLRLF---------- 169
             +E  +  +  + ++    Y    +   ++  +F +++++DGCF+L LF          
Sbjct: 148 HNIEMYIDAMKELEEEARACYQGPID--MKNSNEFTEMLVLDGCFVLELFKGTIQGFQKI 207

Query: 170 ----CDCPASLVNMKMDIRLETLLLENQLPLKLLHKL----------HSIAGPIFISEGD 229
                D   +   +   I+ + ++LENQLPL +L +L            I   + +    
Sbjct: 208 GYARNDPVFAKRGLMHSIQRDMIMLENQLPLFVLDRLLGLQTGTPNQTGIVAEVAVRFFK 267

Query: 230 DERPLQELIC-NWLGISQKDLLDEF-----LHILDMYKASLLYPPIDRTKWCEEGKGKGK 289
              P  E++  +   +  ++  DE      LH LD++  SL+           E   +G 
Sbjct: 268 TLMPTSEVLTKSERSLDSQEKSDELGDNGGLHCLDVFHRSLIQ--------SSETTNQGT 327

Query: 290 GKEKGKEEEKEELSGAEFQVIPPATKLHEAGIKFKMSQTKSLKDVIFDEVEGVLSLPMLM 349
             E     EK++      Q+I   T+L  AG+ F   +T  L D+ F    G L +P L+
Sbjct: 328 PYEDMSMVEKQQ------QLIHCVTELRGAGVNFMRKETGQLWDIEFK--NGYLKIPKLL 387

Query: 350 VDDNTESTFLNVMAFEKLHVEGAAQEAPSLITSFVILMSNLIDDERDVALLSSKGTLANA 409
           + D T+S F N++AFE+ H + +     + ITS++I M NLI+  +DV+ L   G + + 
Sbjct: 388 IHDGTKSLFSNLIAFEQCHTQSS-----NNITSYIIFMDNLINSSQDVSYLHHDGIIEHW 447

Query: 410 LGNDRQAAELFSRLGKGVAMGP-NRHMMAVHKMLNDYCKEPWNERCATLKHQYFQNPWTV 464
           LG+D + A+LF+RL K V   P + ++  + + +N Y    WN   ATL+ +YF NPW  
Sbjct: 448 LGSDSEVADLFNRLCKEVIFDPKDGYLSQLSREVNRYYSRKWNSLKATLRQKYFNNPWAY 506

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SD531.2e-2925.78UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
P0C8971.4e-1729.14Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 ... [more]
Match NameE-valueIdentityDescription
KAG6592830.10.0100.00UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022960231.10.095.09UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022960232.1 UPF0481 prot... [more]
XP_023513985.12.14e-28384.40UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo][more]
KAG7025238.13.03e-26186.93UPF0481 protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023004238.16.11e-18160.65UPF0481 protein At3g47200-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1H6V90.095.09UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111461034 PE=... [more]
A0A6J1KYV82.96e-18160.65UPF0481 protein At3g47200-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1KVQ64.05e-18160.78UPF0481 protein At3g47200-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1HB251.87e-17258.46UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111461183 PE=... [more]
A0A6J1KTE28.48e-15466.11uncharacterized protein LOC111498069 OS=Cucurbita maxima OX=3661 GN=LOC111498069... [more]
Match NameE-valueIdentityDescription
AT3G50120.16.5e-5029.06Plant protein of unknown function (DUF247) [more]
AT3G50170.16.5e-5030.19Plant protein of unknown function (DUF247) [more]
AT3G50130.12.8e-4529.26Plant protein of unknown function (DUF247) [more]
AT3G50160.16.3e-4528.38Plant protein of unknown function (DUF247) [more]
AT3G50150.11.4e-4429.15Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 115..135
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 252..272
NoneNo IPR availablePANTHERPTHR31549:SF29EXPRESSED PROTEINcoord: 48..463
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 54..448
e-value: 3.1E-85
score: 286.9
IPR004158Protein of unknown function DUF247, plantPANTHERPTHR31549PROTEIN, PUTATIVE (DUF247)-RELATED-RELATEDcoord: 48..463

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g009640.m01Csor.00g009640.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane