Csor.00g008890 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g008890
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	rRNA N-glycosidase
Location	Csor_Chr08: 573084 .. 573962 (+)
RNA-Seq Expression	Csor.00g008890
Synteny	Csor.00g008890

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglestart_codonpolypeptidestop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAAACTCATAGCAATCACCTCTTTCTTACTCGCCATCTCTCTTATCTCTCCCACTCTCAAGGCTGACATAGCCTTCGATTTGTCCACAGCTACCAAAGCATCCTATTCAGCATTCATAACCCAGCTCAGGAATGCTCTTCCAACCAAAGCAACAGTGTGCAACATACCCCTGCTGCCTTCCACCGCAACGGGCTTAGGCTGGTTCACATTCTTCACCATCACCAATTATAACTACCAAACCGTCGAGGTGGCTGTGAACGTATCCAACGTCTACATCGTAGCGTATCGTGCCGATGCAAACTCCTACTTTTTCGAAGACACTCCAGCTCAAGCCAAACAACTCTTATTTGCAGGTACTCAAAAGGTAACACTTCCTTTTACGGGTAACTATGATAAGCTCCAAAGCATTGTAGGCAAATATAGAGATATGGTTGAGCTTGGAATCCCCGCCTTGAGCACTGCAGTTACCAACATGGTTTACTACAATAAACAACCGACTGCGGCGGCGCTTCTTGTGCTCATTCAGTCCACTGCTGAAGCTGCGAGGTTTAAATATATTGAGCAACAGGTTGCTCAGTATATTAGCGGTAAGTTTTATCCAAACCAAGCAGTGATAAGCTTAGAGAACAACTGGGGAGCTCTCTCCAAGCAAATCCAGATAGCAAATAGTACTGGAAGTGGACAATTCCAGAGCCCCGTTGAGCTTATAAGTCCTAATGGCACACGATTTAGGGTAACCAGTACATCATCTGGGGTTGTTCAAGGCAATATCAAGCTCCTGCTGTACTACAAACTGAGCGTTGCTGATGACAACGACAGCCCGACAAGAATGCCTGACAATATTGTTGAATATGGAGCTCATGATTCTATGTAG

mRNA sequence

ATGAACAAACTCATAGCAATCACCTCTTTCTTACTCGCCATCTCTCTTATCTCTCCCACTCTCAAGGCTGACATAGCCTTCGATTTGTCCACAGCTACCAAAGCATCCTATTCAGCATTCATAACCCAGCTCAGGAATGCTCTTCCAACCAAAGCAACAGTGTGCAACATACCCCTGCTGCCTTCCACCGCAACGGGCTTAGGCTGGTTCACATTCTTCACCATCACCAATTATAACTACCAAACCGTCGAGGTGGCTGTGAACGTATCCAACGTCTACATCGTAGCGTATCGTGCCGATGCAAACTCCTACTTTTTCGAAGACACTCCAGCTCAAGCCAAACAACTCTTATTTGCAGGTACTCAAAAGGTAACACTTCCTTTTACGGGTAACTATGATAAGCTCCAAAGCATTGTAGGCAAATATAGAGATATGGTTGAGCTTGGAATCCCCGCCTTGAGCACTGCAGTTACCAACATGGTTTACTACAATAAACAACCGACTGCGGCGGCGCTTCTTGTGCTCATTCAGTCCACTGCTGAAGCTGCGAGGTTTAAATATATTGAGCAACAGGTTGCTCAGTATATTAGCGGTAAGTTTTATCCAAACCAAGCAGTGATAAGCTTAGAGAACAACTGGGGAGCTCTCTCCAAGCAAATCCAGATAGCAAATAGTACTGGAAGTGGACAATTCCAGAGCCCCGTTGAGCTTATAAGTCCTAATGGCACACGATTTAGGGTAACCAGTACATCATCTGGGGTTGTTCAAGGCAATATCAAGCTCCTGCTGTACTACAAACTGAGCGTTGCTGATGACAACGACAGCCCGACAAGAATGCCTGACAATATTGTTGAATATGGAGCTCATGATTCTATGTAG

Coding sequence (CDS)

ATGAACAAACTCATAGCAATCACCTCTTTCTTACTCGCCATCTCTCTTATCTCTCCCACTCTCAAGGCTGACATAGCCTTCGATTTGTCCACAGCTACCAAAGCATCCTATTCAGCATTCATAACCCAGCTCAGGAATGCTCTTCCAACCAAAGCAACAGTGTGCAACATACCCCTGCTGCCTTCCACCGCAACGGGCTTAGGCTGGTTCACATTCTTCACCATCACCAATTATAACTACCAAACCGTCGAGGTGGCTGTGAACGTATCCAACGTCTACATCGTAGCGTATCGTGCCGATGCAAACTCCTACTTTTTCGAAGACACTCCAGCTCAAGCCAAACAACTCTTATTTGCAGGTACTCAAAAGGTAACACTTCCTTTTACGGGTAACTATGATAAGCTCCAAAGCATTGTAGGCAAATATAGAGATATGGTTGAGCTTGGAATCCCCGCCTTGAGCACTGCAGTTACCAACATGGTTTACTACAATAAACAACCGACTGCGGCGGCGCTTCTTGTGCTCATTCAGTCCACTGCTGAAGCTGCGAGGTTTAAATATATTGAGCAACAGGTTGCTCAGTATATTAGCGGTAAGTTTTATCCAAACCAAGCAGTGATAAGCTTAGAGAACAACTGGGGAGCTCTCTCCAAGCAAATCCAGATAGCAAATAGTACTGGAAGTGGACAATTCCAGAGCCCCGTTGAGCTTATAAGTCCTAATGGCACACGATTTAGGGTAACCAGTACATCATCTGGGGTTGTTCAAGGCAATATCAAGCTCCTGCTGTACTACAAACTGAGCGTTGCTGATGACAACGACAGCCCGACAAGAATGCCTGACAATATTGTTGAATATGGAGCTCATGATTCTATGTAG

Protein sequence

MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLLPSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAGTQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTAEAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISPNGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM

Homology

[Close]

BLAST of Csor.00g008890 vs. ExPASy Swiss-Prot
Match: P56626 (Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=50544 GN=TCA PE=1 SV=2)

HSP 1 Score: 397.1 bits (1019), Expect = 1.7e-109
Identity = 203/273 (74.36%), Postives = 237/273 (86.81%), Query Frame = 0


Query: 5   IAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLLPSTA 64
           +A++ F LAISL SPT   D++FDLSTATK SYS+FITQLR+ALPT+ TVC IPLLPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  TGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAGTQKV 124
           +G  WF FF +TNYN +TV VAVNV+NVYIVAYRADA SYFFEDTPA+A +L+FAGT+ V
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 TLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTAEAAR 184
            LP++GNYDKLQS+VGK RDM+ELGIPALS+A+TNMVYY+ Q TAAALLVLIQ TAEAAR
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 180

Query: 185 FKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISPNGTR 244
           +KYIEQQV+ +IS  FYPNQAVISLEN WGALSKQIQIAN TG GQF++PVEL +P+GTR
Sbjct: 181 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 240

Query: 245 FRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPT 278
           F VT+TS+GVV+GNIKLLLYYK SV  + D PT
Sbjct: 241 FSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPT 273

[Close]

BLAST of Csor.00g008890 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 272.3 bits (695), Expect = 6.3e-72
Identity = 142/273 (52.01%), Postives = 196/273 (71.79%), Query Frame = 0


Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           M K + ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP    V +IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
            ST +    F    +T+Y Y+T+ VA++V+NVY+VAYR    SYFF+++P +A  +LF G
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           T+K+TLP+TGNY+ LQ+   K R+ ++LG+PALS+A+T + YYN Q   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAARFKYIE+ VA+Y++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDN 274
            G RF+VT+  S VV+GNIKLLL  + S AD+N
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 272

[Close]

BLAST of Csor.00g008890 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 8.2e-72
Identity = 142/273 (52.01%), Postives = 196/273 (71.79%), Query Frame = 0


Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           M K + ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP    V +IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
            ST +    F    +T+Y Y+T+ VA++V+NVY+VAYR    SYFF+++P +A  +LF G
Sbjct: 61  YSTISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           T+K+TLP+TGNY+ LQ+   K R+ ++LG+PALS+A+T + YYN Q   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAARFKYIE+ VA+Y++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDN 274
            G RF+VT+  S VV+GNIKLLL  + S AD+N
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 272

[Close]

BLAST of Csor.00g008890 vs. ExPASy Swiss-Prot
Match: P84531 (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2)

HSP 1 Score: 243.8 bits (621), Expect = 2.4e-63
Identity = 127/240 (52.92%), Postives = 170/240 (70.83%), Query Frame = 0


Query: 24  DIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLLPSTATGLGWFTFFTITNYNYQTV 83
           ++ FDLS+AT +SY  FI  LR ALP    V +IP+L ST      F    + NY+ Q++
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI 60

Query: 84  EVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAGTQKVTLPFTGNYDKLQSIVGKYR 143
             A++V NVYIVAY     SYFF+  PAQA +LLF GTQ+ TLP+TGNY+ LQ+   K R
Sbjct: 61  TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR 120

Query: 144 DMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTAEAARFKYIEQQVAQYISGKFYPN 203
           + +ELG+PAL +A+T + +YN +  A+ALLVLIQ+T+EAARF+YIE Q+A  +  KF P+
Sbjct: 121 ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS 180

Query: 204 QAVISLENNWGALSKQIQIANSTGSGQFQSPVELISPNGTRFRVTSTSSGVVQGNIKLLL 263
           Q +ISLENNW ALSKQIQIA +  +GQF++PV LI P G R ++T+ +S VV  NI+LLL
Sbjct: 181 QTIISLENNWSALSKQIQIAKNK-NGQFETPVILIDPQGNRVQITNVTSNVVTQNIQLLL 239

[Close]

BLAST of Csor.00g008890 vs. ExPASy Swiss-Prot
Match: P33185 (Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3)

HSP 1 Score: 241.1 bits (614), Expect = 1.5e-62
Identity = 136/265 (51.32%), Postives = 180/265 (67.92%), Query Frame = 0


Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           M KL+ +   +L I L SPT++ D++F LS AT  SY  FI  LR ALP +  V NIPLL
Sbjct: 1   MIKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYERKVYNIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPA--QAKQLLF 120
            S+ +G G +T   +TNY  +T+ VAV+V+NVYI+ Y A   SYFF +  A   AK +  
Sbjct: 61  RSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGDVSYFFNEASATEAAKFVFK 120

Query: 121 AGTQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQS 180
              +KVTLP++GNY++LQ+  GK R+ + LG+PAL +A+T + YY     A+ALLVLIQS
Sbjct: 121 DAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYYTASSAASALLVLIQS 180

Query: 181 TAEAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELI 240
           TAE+AR+K+IEQQ+ + +   F P+ A ISLENNW ALSKQIQIA ST +GQF+SPV LI
Sbjct: 181 TAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIA-STNNGQFESPVVLI 240

Query: 241 SPNGTRFRVTSTSSGVVQGNIKLLL 264
             N  R  +T+ S+ VV  NI LLL
Sbjct: 241 DGNNQRVSITNASARVVTSNIALLL 264

[Close]

BLAST of Csor.00g008890 vs. NCBI nr
Match: KAG6592904.1 (Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025309.1 Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 564 bits (1454), Expect = 1.75e-202
Identity = 292/292 (100.00%), Postives = 292/292 (100.00%), Query Frame = 0


Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
           PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG
Sbjct: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP
Sbjct: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM 292
           NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM
Sbjct: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM 292

[Close]

BLAST of Csor.00g008890 vs. NCBI nr
Match: XP_022960460.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita moschata])

HSP 1 Score: 531 bits (1369), Expect = 1.58e-189
Identity = 275/292 (94.18%), Postives = 283/292 (96.92%), Query Frame = 0


Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           MNKLIAITSFLLAISLISPTLKADIA DLSTATKA+YSAFITQLRNALPTKATVCNIPLL
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIALDLSTATKATYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
           PSTATGLG FTFFTITNYNY+TVEVAVNVSNVYIVAYRA+A SYFFEDT AQA+QLLF  
Sbjct: 61  PSTATGLGSFTFFTITNYNYETVEVAVNVSNVYIVAYRANAKSYFFEDTSAQARQLLFQN 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           TQK+TLPFTGNYD+LQ +VGKYRD +ELGIPALSTAVTNMVYYNKQ TAAALLVLIQSTA
Sbjct: 121 TQKITLPFTGNYDRLQGVVGKYRDSIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSTA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP
Sbjct: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM 292
           NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM
Sbjct: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM 292

[Close]

BLAST of Csor.00g008890 vs. NCBI nr
Match: XP_023513430.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 523 bits (1348), Expect = 2.51e-186
Identity = 273/292 (93.49%), Postives = 280/292 (95.89%), Query Frame = 0


Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           MNKLIAI+SFLLAISLISPTLKADIA DLSTATKASYSAFIT +RNALPTKATVCNIPLL
Sbjct: 1   MNKLIAISSFLLAISLISPTLKADIALDLSTATKASYSAFITHIRNALPTKATVCNIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
           PST TGL  FTFFTITNYNYQTVEVAVNV+NVYIVAYRA A+SYFFEDTPAQAKQLLFAG
Sbjct: 61  PSTPTGLASFTFFTITNYNYQTVEVAVNVTNVYIVAYRAGASSYFFEDTPAQAKQLLFAG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQ TAAALLVLIQSTA
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQLTAAALLVLIQSTA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAARFKYIEQQVAQ+IS KFYPNQAVISLENNWGALSKQIQIANSTG GQFQSPVELI P
Sbjct: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIGP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM 292
            GTRF VT+TS+GVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM
Sbjct: 241 TGTRFSVTNTSAGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM 292

[Close]

BLAST of Csor.00g008890 vs. NCBI nr
Match: XP_023005055.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima])

HSP 1 Score: 523 bits (1346), Expect = 5.06e-186
Identity = 268/292 (91.78%), Postives = 281/292 (96.23%), Query Frame = 0


Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           MNKLIAITSFL+AISLISPTLKADI FDLSTATKASYSAFITQLRNALPT ATVCNIPLL
Sbjct: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
           PSTA+GLGWFTFFTITNYNYQT+ VAVNV+NVYIVAYRADA+SYFFEDTPA+AKQLLF  
Sbjct: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           TQKVTLPFTGNYDKLQSIVGKYRD++ELGIPALSTAVTNMVYYNKQ TAAALLVLIQS+A
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAARFKYIEQQVAQ+IS KFYPNQAVISLENNWGALSKQIQIANSTG GQFQSPVELISP
Sbjct: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM 292
           NGTRF VTSTS+GVVQ NIKLLLYYKLSVAD+NDSPTRMPDNIVEYG HDS+
Sbjct: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKLSVADENDSPTRMPDNIVEYGTHDSV 292

[Close]

BLAST of Csor.00g008890 vs. NCBI nr
Match: P56626.2 (RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; AltName: Full=Trichoanguin; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Trichosanthes anguina] >AAD02686.1 trichoanguin [Trichosanthes cucumerina])

HSP 1 Score: 398 bits (1023), Expect = 8.24e-137
Identity = 203/272 (74.63%), Postives = 236/272 (86.76%), Query Frame = 0


Query: 6   AITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLLPSTAT 65
           A++ F LAISL SPT   D++FDLSTATK SYS+FITQLR+ALPT+ TVC IPLLPSTA+
Sbjct: 2   ALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTAS 61

Query: 66  GLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAGTQKVT 125
           G  WF FF +TNYN +TV VAVNV+NVYIVAYRADA SYFFEDTPA+A +L+FAGT+ V 
Sbjct: 62  GSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTVK 121

Query: 126 LPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTAEAARF 185
           LP++GNYDKLQS+VGK RDM+ELGIPALS+A+TNMVYY+ Q TAAALLVLIQ TAEAAR+
Sbjct: 122 LPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAARY 181

Query: 186 KYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISPNGTRF 245
           KYIEQQV+ +IS  FYPNQAVISLEN WGALSKQIQIAN TG GQF++PVEL +P+GTRF
Sbjct: 182 KYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTRF 241

Query: 246 RVTSTSSGVVQGNIKLLLYYKLSVADDNDSPT 277
            VT+TS+GVV+GNIKLLLYYK SV  + D PT
Sbjct: 242 SVTNTSAGVVKGNIKLLLYYKASVGSEYDIPT 273

[Close]

BLAST of Csor.00g008890 vs. ExPASy TrEMBL
Match: A0A6J1H951 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111461188 PE=3 SV=1)

HSP 1 Score: 531 bits (1369), Expect = 7.65e-190
Identity = 275/292 (94.18%), Postives = 283/292 (96.92%), Query Frame = 0


Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           MNKLIAITSFLLAISLISPTLKADIA DLSTATKA+YSAFITQLRNALPTKATVCNIPLL
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIALDLSTATKATYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
           PSTATGLG FTFFTITNYNY+TVEVAVNVSNVYIVAYRA+A SYFFEDT AQA+QLLF  
Sbjct: 61  PSTATGLGSFTFFTITNYNYETVEVAVNVSNVYIVAYRANAKSYFFEDTSAQARQLLFQN 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           TQK+TLPFTGNYD+LQ +VGKYRD +ELGIPALSTAVTNMVYYNKQ TAAALLVLIQSTA
Sbjct: 121 TQKITLPFTGNYDRLQGVVGKYRDSIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSTA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP
Sbjct: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM 292
           NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM
Sbjct: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM 292

[Close]

BLAST of Csor.00g008890 vs. ExPASy TrEMBL
Match: A0A6J1KWC4 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498162 PE=3 SV=1)

HSP 1 Score: 523 bits (1346), Expect = 2.45e-186
Identity = 268/292 (91.78%), Postives = 281/292 (96.23%), Query Frame = 0


Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           MNKLIAITSFL+AISLISPTLKADI FDLSTATKASYSAFITQLRNALPT ATVCNIPLL
Sbjct: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
           PSTA+GLGWFTFFTITNYNYQT+ VAVNV+NVYIVAYRADA+SYFFEDTPA+AKQLLF  
Sbjct: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           TQKVTLPFTGNYDKLQSIVGKYRD++ELGIPALSTAVTNMVYYNKQ TAAALLVLIQS+A
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAARFKYIEQQVAQ+IS KFYPNQAVISLENNWGALSKQIQIANSTG GQFQSPVELISP
Sbjct: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM 292
           NGTRF VTSTS+GVVQ NIKLLLYYKLSVAD+NDSPTRMPDNIVEYG HDS+
Sbjct: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKLSVADENDSPTRMPDNIVEYGTHDSV 292

[Close]

BLAST of Csor.00g008890 vs. ExPASy TrEMBL
Match: A0A6J1DUC7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023554 PE=3 SV=1)

HSP 1 Score: 370 bits (950), Expect = 3.67e-126
Identity = 190/281 (67.62%), Postives = 232/281 (82.56%), Query Frame = 0


Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           M K IA++ F+L I L  PT K D++FDLSTATK SY++FI  LR+ALPT+ TVCNIP+L
Sbjct: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
           PSTA+GL WF FF++TNYNYQT+ VAVNV+NVYI AY   A SYFFEDT A+A +LLF G
Sbjct: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQP-TAAALLVLIQST 180
           TQKVTLP++GNYD+LQSIVGK RD++ELGIP LS A+TN+ Y N    TA ALLVLIQST
Sbjct: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180

Query: 181 AEAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELIS 240
           AEAARFKYIEQ+V++++S  F+PNQA+ISLEN+WGALSKQIQIANS G+GQF   VELI+
Sbjct: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240

Query: 241 PNGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMP 280
            +G R  VT+TS+GVV+ NIKLLLYYK++ A+DN  PT +P
Sbjct: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILP 281

[Close]

BLAST of Csor.00g008890 vs. ExPASy TrEMBL
Match: A0A6J1DR40 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023561 PE=3 SV=1)

HSP 1 Score: 317 bits (812), Expect = 4.12e-105
Identity = 172/284 (60.56%), Postives = 215/284 (75.70%), Query Frame = 0


Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           M KL  + S LLAI L   + + D++F LS A K SY+ FI QLR+A+PT  TVC IP+L
Sbjct: 1   MRKLTTL-SLLLAILLGPLSAEGDVSFQLSGANKKSYTNFIKQLRDAVPTTTTVCKIPVL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
           PSTA+G   FTF  +TNY  QTV VA+N++NVYIVAY  D  SYFF+D   +A ++LF G
Sbjct: 61  PSTASGPKSFTFVHLTNYADQTVVVAINITNVYIVAYHIDHVSYFFDDASPEAYKVLFEG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           T + TLP++ NYDKLQSIVGK RD++ELG+PALS+A+TN+VYY  + TAAALLVLIQS A
Sbjct: 121 TDRKTLPYSSNYDKLQSIVGKKRDLIELGLPALSSAITNLVYYEYKSTAAALLVLIQSVA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAAR+KYIEQ+++ ++  KF P+QA+ISLENNWGALSKQIQIA + G+GQF+ PVELI+P
Sbjct: 181 EAARYKYIEQEISTHVDDKFKPDQAIISLENNWGALSKQIQIA-AKGNGQFEKPVELINP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVA--DDNDSPTRMPDN 282
           N T F VT+ SS VV  NIKLLLY+KL+ A  DD D     P N
Sbjct: 241 NNTPFSVTNVSSLVVTSNIKLLLYFKLAAANHDDQDKTVGKPFN 282

[Close]

BLAST of Csor.00g008890 vs. ExPASy TrEMBL
Match: S6CNA6 (rRNA N-glycosidase (Fragment) OS=Momordica charantia OX=3673 GN=rip PE=3 SV=1)

HSP 1 Score: 275 bits (702), Expect = 1.35e-88
Identity = 144/266 (54.14%), Postives = 194/266 (72.93%), Query Frame = 0


Query: 9   SFLL-AISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLLPSTATGL 68
           SFL+ AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP    V +IPLL ST +  
Sbjct: 2   SFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLLYSTISDS 61

Query: 69  GWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAGTQKVTLP 128
             F    +T+Y Y+T+ VA++V+NVY+VAYR    SYFF+++P +A  +LF GT+K+TLP
Sbjct: 62  RRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLP 121

Query: 129 FTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTAEAARFKY 188
           +TGNY+ LQ+   K R+ ++LG+PALS+A+T + YYN QP  +ALLVLIQ+TAEAARFKY
Sbjct: 122 YTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQPAPSALLVLIQTTAEAARFKY 181

Query: 189 IEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISPNGTRFRV 248
           IE+ VA+Y++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P G RF+V
Sbjct: 182 IERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQV 241

Query: 249 TSTSSGVVQGNIKLLLYYKLSVADDN 273
           T+  S VV+GNIKLLL  + S AD+N
Sbjct: 242 TNVDSDVVKGNIKLLLNSRASTADEN 266

The following BLAST results are available for this feature:

BLAST of Csor.00g008890 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P56626	1.7e-109	74.36	Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=5...	[more]
P24817	6.3e-72	52.01	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P29339	8.2e-72	52.01	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
P84531	2.4e-63	52.92	Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2	[more]
P33185	1.5e-62	51.32	Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3	[more]

BLAST of Csor.00g008890 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6592904.1	1.75e-202	100.00	Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrospe...	[more]
XP_022960460.1	1.58e-189	94.18	type I ribosome-inactivating protein trichoanguina-like [Cucurbita moschata]	[more]
XP_023513430.1	2.51e-186	93.49	type I ribosome-inactivating protein trichoanguina-like [Cucurbita pepo subsp. p...	[more]
XP_023005055.1	5.06e-186	91.78	type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima]	[more]
P56626.2	8.24e-137	74.63	RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; Alt...	[more]

BLAST of Csor.00g008890 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H951	7.65e-190	94.18	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111461188 PE=3 SV=1	[more]
A0A6J1KWC4	2.45e-186	91.78	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498162 PE=3 SV=1	[more]
A0A6J1DUC7	3.67e-126	67.62	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023554 PE=3 SV=1	[more]
A0A6J1DR40	4.12e-105	60.56	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023561 PE=3 SV=1	[more]
S6CNA6	1.35e-88	54.14	rRNA N-glycosidase (Fragment) OS=Momordica charantia OX=3673 GN=rip PE=3 SV=1	[more]

BLAST of Csor.00g008890 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 172..192 score: 66.67 coord: 82..97 score: 44.74 coord: 143..157 score: 40.7 coord: 204..221 score: 52.63 coord: 35..48 score: 45.3
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 184..270 e-value: 1.6E-29 score: 103.6
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 24..183 e-value: 2.4E-43 score: 149.6
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 33..221 e-value: 9.4E-33 score: 113.5
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 25..265
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 25..265
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 176..192
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 24..268

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g008890.m01	Csor.00g008890.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity