Csor.00g004540 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g004540
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionCitrate synthase
LocationCsor_Chr08: 3296822 .. 3301848 (-)
RNA-Seq ExpressionCsor.00g004540
SyntenyCsor.00g004540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGAGCTCACCGGATCTTGTAATATTCTCCACTCACATTAATCTTTATTCATCAATCTTGTTTGGGTAGACTACCGTTTTAAGGCTTTGAGACTTCATTTTGGGGTAGATCTAATAATTATTAATTGTTTAAATCGGACTGTGACGGCTACATTTCCCTGCTAGCCAGAGTTGATAACTGTTGACATTAAGTCTACGTGCATATTTCCAATTGGTTGATCTTTGGCAATTGAGTAGTCAGTTACCTAACAGATGGAATGATTACTTCTATCCAATAAGTGATTTCTCTTATAAGTTCGGTTTGTCGTGCAGGACCTACAATCTCATTTGAGGGAATTAATTCCAGAACAGCAGGTTTGGTTATCCTAACAGTATTCATTTAATAATTATTTAGCATAGCACTCCCACAATAAATACGAAGGCCTTCGTTCTTATTCTTTAAAGTTTTACACTAACGTCCAAGTTGATTCACTCATCTGCCTTTCATTGAGTATGACCACTTCACTGAAATGGGATGCATGTTTTTGACATAAATTCATCAGGAGATTACTTTAGATGCTTTTTTCCTGTATCCAGTTTGGTTGAAAGTTTTATATACAACAGTGTGACTAATTGATTCAATGCATTTACTGCCGACCCTGACCTAATTTTCCCCGATAATTATATAGTTCATTTATATATATTCATAATATGTTTAATGATTATAGGATCGTCTGAAGAAATTCAAAGCAGAACATGGAAAGGTTCAACTGGGCAACATCACTGTTGATATGGTAAGTATATTTGCTGAACTATTCTATATTTTGCTGCTCTCTAACATCGGTGTCATTTAGTCCCATGTGACAAATTCGTTCCAGGGAGCTCTTATGAAAAATAATCTCTGATCTTTTCTGCTAGCATTTTCTTTACATCTCTTAATAAATGCCTTGTCTCGAACATTGTGAAGGTACTTGGTGGAATGAGAGGAATGACAGGGCTACTGTGGGAAACATCTCTACTTGACCCTGACGAGGTTTAGAATCCTGTTGGTTAATACCTAGTGAATTGTTTTGGTCTTTAAATGTCATTTGTTAAGGTTTCTGTGCTCAACATTCATGGTTATCTCTGGTCTATATGAATTGGATGAATGCTGGTTTTAGTATAGTAATGGATTTGGTGTATTATATTTCCAGGGTATTCGCTTTAGGGGTTTATCAATTCCTGAATGTCAAAAATTACTACCAGCTGCAAAACCTGATGGAGAGCCACTACCTGAGGGTCTCCTGTGGCTTCTTCTGACAGGAAAGGTGAGTTGCCAAAAACTTGTCCATTCGTTTGAACTTTTGGTAATTATATCAGAACTGTAGAGGCATCAGACATGTAGCTGATAGAATAAACCTACTTCTCCTTGTAGGCAGTATTTTGCTTTATACTTCCAAAAACATCATCATTTATTCTTTTATATATCAGGTACCAAGCAAAGAGCAAGTAGATGCACTATCCAAGGAGTTACAAAGCCGAGCTGTTGTTCCAGGTTCAGCTTGTTCATGATTGTTGCATGAGCATATTTGATTATATATGACTTCCGGAAGCCTCCTCTGCTCCAGTAGACATTTTCCTAAATGGTGGGTTTTTGTCATTTCTGGTTGCAGATTATGTATACAAGGCCATCGATGCTCTTCCCGTTACATCTCATCCGATGACTCAGTTTGCCACAGGTGTTATGGGTCTTCAGGTTAGTAATGAGTTCTCTATTTATCACGCCCTAGATGAGGTGATGATTTTCTTTGTCAACTTTACGTGAAAGGTTTTTGTATGGATACTTGGATCAATTTGGAAATTTCATATTTGACTTCCATAAATACTTTTTAGCATCCAACTTCTCGTTTGTTTATTTTGATCAAAACACAGCGTTTTATGCTTGGATCCAATTTTATAAACCCTGTACTGCTGAATTTAGTTTGTTGGATTATATTGAGAAAAAGTTTAGTTTATTAAAAACGTAATTAATTATCTAGGTGCACTGTTGTTAGCTGTCTTTTAAGGTGTCTACTTGTACTTTCCTTCAGGTTCAAAGTGAATTCCCGAAGGCTTATGAGAAAGGAATTCATAAATCAAAGTAAAGTTCGTGCATTCAAGATTCAGTCTTCGTATTTTCTCAAGTACTGAAAGCTATTTAATTTTATAGGTACTGGGAACCAACGTATGAAGATTCTATGAATTTGGTTGCTCGAGTACCTTTGGTAGCTTCTTATGTCTACCGCAGGTTGGTAGATCACTCTAATTATATATTTGTTGATTGTATGAAACTAATTGTGTCACTTGCCGTTGCATATTTAAAATATTCTGACCCCTTCTTGCACGGTCTCCTCCATTTCGTTGTTGGATTAATTTGGACTTCCTTCTAATCATATTTGAAGGATATACAAGGATGGCCATATAATTCCAAAAGATGACTCTCTGGATTATGGTGGCAATTTTTCGCACATGTTAGGATTTGATAGTCCCCAAATGCAAGAACTCATGAGGCTCTATGTTACTATCCATACGTAAGTATATGTTGTTCATTTCCATCGGATTTAAAGTATTTTAATTTAAGAGAGAATAATCAAATAACATCTAATGTTTGTGTAAAAATGACCTGCAGTGATCATGAAGGAGGGAATGTCAGCGCTCACACAGGCCACCTCGTAAGTTATCTTTCTTCTCAGATTTCAATTGAATTGTGTGTTTTCTTTTCTCTTTAGTATTTTATAGTATCGTTTCCTTAGTCTGAACTTCTGCTGTACATTGATTGAAACATGACTTTTTCAAGCGTAGCTGTCAGTGTAATGTTTCTGCACATCAAATTCATGCCTGTGTCATTCTCTTAGTTATTTAATACTTTTATGCAGGTTGCTAGTGCACTTTCAGATCCCTATCTTTCGTTTGCTGCTGCTCTAAACGGATTGGCTGGACCATTACATGGTTTGGCAAATCAGGTGTGTGATAGACTGTTTTCTAATAGCTACTCCTTTTCCTCTATTTGGTTTGAACTTTTAATAAAGTACGTAATTATCATTTGTAACTCTCTCATTATATTGATGGATTTCATTCTTACTACTATTTTCCCCCCTCAATTCATTGACTTTCAATTTATCTAGAAAGTGTAAAATAGAAATCAATAGATAAGTGAAAGGGATTGAGATAATTATTTGGTTTACTCATCATAACTGATAACAGTCTAATAGTACCTTATGGAACATTCTTCAGGAATGTTTTTGATTCTGCTATTTATCTTTGGAGTGAGGGAACTTGTGTTGTTCATTTTACGTCTGTAGATTTGAAACCAAGTAATGTTTGAGCGTTAGCCTTAAACTAACTGCAATTAACTTTATGACATGAATATACATAGCAAATGTTTGCTCTATGCATCTTATCAACATATAAAGATTGCCAATTATTGTTGCTTATTTCCCACTGCTTGCAGGAAGTTTTACTTTGGATCAAATCTGTAGTGGACGAGTGTGGAGAGAACATAACTAAGGAGCAGTTGAAAGACTATGTTTGGAAAACTCTAAACAGTGGCAAGGTAACAACATAAATCCATTCTGGCTAGAAATGTCAGGCATGGAGCTTATACTTAATATACCTTCATCCTCGCTTATATATAGCTAGTATCATTAACTGTACTTCTGTTTGAGAATAACAATTGAATGGTTATTTTGGTAGGAATATGCATTCTTAGACTTTTTTTGTTGGTTCATTAATCCTCCTCTCTCAGTTTGCCGCTGTTTGTCAAAACTTTCCTTCAAGCATTTCAGTCATAGTGGTTTATTTTTCTGGGAGATATTTTTTTTCCCGTTTTCAAAAACTCCAATGTATTTCGAGTCTTATCCTCTATAGGAAGAGGAGACGTCCTTTATGGATTTCTTTTTCAAACTTATGGTTACATGATTTGTGGAACTTTTATCAGCTCCTTATCATTCTTTTTAAATTGTAGGTTGTTCCAGGATTTGGTCACGGAGTTCTGCGTAAAACAGATCCAAGATATACTTGTCAAAGAGAGTTTGCATTGAAGCATTTGCCAGACGATCCTCTTTTTCAGCTGGTTGTCATTTTCTCCCTGATTGTAGACTCACACACAAGCATTCATACACACGCACTGTATAAATCCCTTTTGGTTTACTTAAGCCGTAAGTTGCTCACACAATAACCTAGCATGGGATTTGCAGGTATCCAAGCTCTACGAAGTCGTGCCACCCATCCTAACAGAACTTGGCAAGGCAAGATGAGCTTCCTTCTCTATAACTAGCTCAATTGTGCATGCTCCTTATCTTCTGTTGTTAGTCATTTCTGCTAGCATTTGTGGGTTTATTTCTCAACTCGGGTGGGGGAGTTTCAGTAGTATCAAATTCTTCTTATGATAATTTCTACTGTGTCGACCAAACATATAGATTCCATGAAACTGATTCTTTCCCTTCATAAAATGAATTAAATGTTCTAAGTTTGTACTAAATGTCCAAGATCGAGCACAATATGTAACTTGACTTTTGAGTCAAACACAGTATAATTTGATGGAGTACATGAAACTATCCCATGAATTGTTGATGCTCACGAACGTCACCCATGAATCTTACATAGTTGTCTCCTCTTGTGGCATTTTCCGCGTTTGCAGGTTAAAAACCCATGGCCTAATGTTGATGCTCATAGTGGTGTGCTGTTGAACTACTTTGGTTTGACCGAAGCAAGGTAGCGATTCATTAATACACATTGATGTTTACTTTTAACTAAACGATAACTATAACTTTCATCAACTATGCACAGGTACTTTACTGTTCTCTTCGGTGTTTCAAGGAGTATTGGGATATGTTCTCAGGTCTGTCTTTTTTTTTTTTTTTTGTACATTCATTCTCAGTTCTTCTTCATTTTACTAAATTCTTAGAGATTTACTACGTGTTCTGCCTTCAGTTAATATGGGACCGAGCTCTGGGGTTGCCACTCGAGAGGCCAAAGAGCGTGACATTGAAATGGCTCGAGGACCACTGCAAGAAAGCAGCAGCTTGA

mRNA sequence

ATGCAGAGCTCACCGGATCTTGACCTACAATCTCATTTGAGGGAATTAATTCCAGAACAGCAGGATCGTCTGAAGAAATTCAAAGCAGAACATGGAAAGGTTCAACTGGGCAACATCACTGTTGATATGGTACTTGGTGGAATGAGAGGAATGACAGGGCTACTGTGGGAAACATCTCTACTTGACCCTGACGAGGGTATTCGCTTTAGGGGTTTATCAATTCCTGAATGTCAAAAATTACTACCAGCTGCAAAACCTGATGGAGAGCCACTACCTGAGGGTCTCCTGTGGCTTCTTCTGACAGGAAAGGTACCAAGCAAAGAGCAAGTAGATGCACTATCCAAGGAGTTACAAAGCCGAGCTGTTGTTCCAGATTATGTATACAAGGCCATCGATGCTCTTCCCGTTACATCTCATCCGATGACTCAGTTTGCCACAGGTGTTATGGGTCTTCAGGTTCAAAGTGAATTCCCGAAGGCTTATGAGAAAGGAATTCATAAATCAAAGTACTGGGAACCAACGTATGAAGATTCTATGAATTTGGTTGCTCGAGTACCTTTGGTAGCTTCTTATGTCTACCGCAGGATATACAAGGATGGCCATATAATTCCAAAAGATGACTCTCTGGATTATGGTGGCAATTTTTCGCACATGTTAGGATTTGATAGTCCCCAAATGCAAGAACTCATGAGGCTCTATGTTACTATCCATACTGATCATGAAGGAGGGAATGTCAGCGCTCACACAGGCCACCTCGTTGCTAGTGCACTTTCAGATCCCTATCTTTCGTTTGCTGCTGCTCTAAACGGATTGGCTGGACCATTACATGGTTTGGCAAATCAGGAAGTTTTACTTTGGATCAAATCTGTAGTGGACGAGTGTGGAGAGAACATAACTAAGGAGCAGTTGAAAGACTATGTTTGGAAAACTCTAAACAGTGGCAAGGTTGTTCCAGGATTTGGTCACGGAGTTCTGCGTAAAACAGATCCAAGATATACTTGTCAAAGAGAGTTTGCATTGAAGCATTTGCCAGACGATCCTCTTTTTCAGCTGGTATCCAAGCTCTACGAAGTCGTGCCACCCATCCTAACAGAACTTGGCAAGGTTAAAAACCCATGGCCTAATGTTGATGCTCATAGTGGTGTGCTGTTGAACTACTTTGGTTTGACCGAAGCAAGGTACTTTACTGTTCTCTTCGGTGTTTCAAGGAGTATTGGGATATGTTCTCAGTTAATATGGGACCGAGCTCTGGGGTTGCCACTCGAGAGGCCAAAGAGCGTGACATTGAAATGGCTCGAGGACCACTGCAAGAAAGCAGCAGCTTGA

Coding sequence (CDS)

ATGCAGAGCTCACCGGATCTTGACCTACAATCTCATTTGAGGGAATTAATTCCAGAACAGCAGGATCGTCTGAAGAAATTCAAAGCAGAACATGGAAAGGTTCAACTGGGCAACATCACTGTTGATATGGTACTTGGTGGAATGAGAGGAATGACAGGGCTACTGTGGGAAACATCTCTACTTGACCCTGACGAGGGTATTCGCTTTAGGGGTTTATCAATTCCTGAATGTCAAAAATTACTACCAGCTGCAAAACCTGATGGAGAGCCACTACCTGAGGGTCTCCTGTGGCTTCTTCTGACAGGAAAGGTACCAAGCAAAGAGCAAGTAGATGCACTATCCAAGGAGTTACAAAGCCGAGCTGTTGTTCCAGATTATGTATACAAGGCCATCGATGCTCTTCCCGTTACATCTCATCCGATGACTCAGTTTGCCACAGGTGTTATGGGTCTTCAGGTTCAAAGTGAATTCCCGAAGGCTTATGAGAAAGGAATTCATAAATCAAAGTACTGGGAACCAACGTATGAAGATTCTATGAATTTGGTTGCTCGAGTACCTTTGGTAGCTTCTTATGTCTACCGCAGGATATACAAGGATGGCCATATAATTCCAAAAGATGACTCTCTGGATTATGGTGGCAATTTTTCGCACATGTTAGGATTTGATAGTCCCCAAATGCAAGAACTCATGAGGCTCTATGTTACTATCCATACTGATCATGAAGGAGGGAATGTCAGCGCTCACACAGGCCACCTCGTTGCTAGTGCACTTTCAGATCCCTATCTTTCGTTTGCTGCTGCTCTAAACGGATTGGCTGGACCATTACATGGTTTGGCAAATCAGGAAGTTTTACTTTGGATCAAATCTGTAGTGGACGAGTGTGGAGAGAACATAACTAAGGAGCAGTTGAAAGACTATGTTTGGAAAACTCTAAACAGTGGCAAGGTTGTTCCAGGATTTGGTCACGGAGTTCTGCGTAAAACAGATCCAAGATATACTTGTCAAAGAGAGTTTGCATTGAAGCATTTGCCAGACGATCCTCTTTTTCAGCTGGTATCCAAGCTCTACGAAGTCGTGCCACCCATCCTAACAGAACTTGGCAAGGTTAAAAACCCATGGCCTAATGTTGATGCTCATAGTGGTGTGCTGTTGAACTACTTTGGTTTGACCGAAGCAAGGTACTTTACTGTTCTCTTCGGTGTTTCAAGGAGTATTGGGATATGTTCTCAGTTAATATGGGACCGAGCTCTGGGGTTGCCACTCGAGAGGCCAAAGAGCGTGACATTGAAATGGCTCGAGGACCACTGCAAGAAAGCAGCAGCTTGA

Protein sequence

MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSLLDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSRAVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMNLVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITKEQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVPPILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLPLERPKSVTLKWLEDHCKKAAA
Homology
BLAST of Csor.00g004540 vs. ExPASy Swiss-Prot
Match: P49298 (Citrate synthase, mitochondrial OS=Citrus maxima OX=37334 GN=CIT PE=2 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 2.5e-237
Identity = 392/440 (89.09%), Postives = 419/440 (95.23%), Query Frame = 0

Query: 1   MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 60
           MQSS DLDL S L+E+IPEQQ+RLKK K++ GK QLGNIT+D+V+GGMRGMTGLLWETSL
Sbjct: 32  MQSSADLDLHSQLKEMIPEQQERLKKVKSDLGKAQLGNITIDVVIGGMRGMTGLLWETSL 91

Query: 61  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 120
           LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVD LSKEL+ R
Sbjct: 92  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDGLSKELRDR 151

Query: 121 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN 180
           A VPDYVYKAIDALPV++HPMTQFA+GVM LQVQSEF +AYEKGIHKSK WEPT EDS+N
Sbjct: 152 ATVPDYVYKAIDALPVSAHPMTQFASGVMALQVQSEFQEAYEKGIHKSKSWEPTSEDSLN 211

Query: 181 LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 240
           L+ARVP+VA+YVY+RIYKDG IIPKDDSLDYGGNFSHMLGFD P+M ELMRLYVTIH+DH
Sbjct: 212 LIARVPVVAAYVYQRIYKDGKIIPKDDSLDYGGNFSHMLGFDDPKMLELMRLYVTIHSDH 271

Query: 241 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 300
           EGGNVSAHTGHLVASALSDPYLSF AALNGLAGPLHGLANQEVLLWIKSVVDECGEN+T 
Sbjct: 272 EGGNVSAHTGHLVASALSDPYLSFLAALNGLAGPLHGLANQEVLLWIKSVVDECGENVTT 331

Query: 301 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 360
           EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP
Sbjct: 332 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 391

Query: 361 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 420
           PILT+LGKVKNPWPNVDAHSGVLLN+FGL EARY+TVLFGVSRS+GICSQLIWDRALGLP
Sbjct: 392 PILTKLGKVKNPWPNVDAHSGVLLNHFGLAEARYYTVLFGVSRSLGICSQLIWDRALGLP 451

Query: 421 LERPKSVTLKWLEDHCKKAA 441
           LERPKSVTL W+E +CKKAA
Sbjct: 452 LERPKSVTLDWIEKNCKKAA 471

BLAST of Csor.00g004540 vs. ExPASy Swiss-Prot
Match: O80433 (Citrate synthase, mitochondrial OS=Daucus carota OX=4039 GN=CS PE=2 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 7.4e-234
Identity = 380/438 (86.76%), Postives = 417/438 (95.21%), Query Frame = 0

Query: 3   SSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSLLD 62
           S+ DLDL+S L+ELIPEQQ+R+KK KAEHGKVQLGNITVDMVLGGMRGMTGLLWETSLLD
Sbjct: 35  SASDLDLRSQLKELIPEQQERIKKLKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSLLD 94

Query: 63  PDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSRAV 122
           P+EGIRFRGLSIPECQKLLP AKP GEPLPEGLLWLLLTGKVP+KEQVDALS EL+SRA 
Sbjct: 95  PEEGIRFRGLSIPECQKLLPGAKPGGEPLPEGLLWLLLTGKVPTKEQVDALSAELRSRAA 154

Query: 123 VPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMNLV 182
           VP++VYK IDALPVT+HPMTQFATGVM LQVQSEF KAYEKGIHK+KYWEPTYEDS+ L+
Sbjct: 155 VPEHVYKTIDALPVTAHPMTQFATGVMALQVQSEFQKAYEKGIHKTKYWEPTYEDSITLI 214

Query: 183 ARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDHEG 242
           A++P+VA+Y+YRR+YK+G  I  DDSLDYG NF+HMLG+DSP MQELMRLYVTIHTDHEG
Sbjct: 215 AQLPVVAAYIYRRMYKNGQSISTDDSLDYGANFAHMLGYDSPSMQELMRLYVTIHTDHEG 274

Query: 243 GNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITKEQ 302
           GNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVV ECGEN+TKEQ
Sbjct: 275 GNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVSECGENVTKEQ 334

Query: 303 LKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVPPI 362
           LKDY+WKTLNSGKVVPG+GHGVLR TDPRY CQREFALKHLPDDPLFQLVS L+EVVPPI
Sbjct: 335 LKDYIWKTLNSGKVVPGYGHGVLRNTDPRYICQREFALKHLPDDPLFQLVSNLFEVVPPI 394

Query: 363 LTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLPLE 422
           LTELGKVKNPWPNVDAHSGVLLN++GLTEARY+TVLFGVSR+IGICSQL+WDRALGLPLE
Sbjct: 395 LTELGKVKNPWPNVDAHSGVLLNHYGLTEARYYTVLFGVSRAIGICSQLVWDRALGLPLE 454

Query: 423 RPKSVTLKWLEDHCKKAA 441
           RPKSVT++WLE+HCKK++
Sbjct: 455 RPKSVTMEWLENHCKKSS 472

BLAST of Csor.00g004540 vs. ExPASy Swiss-Prot
Match: P20115 (Citrate synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CSY4 PE=1 SV=3)

HSP 1 Score: 795.8 bits (2054), Expect = 2.5e-229
Identity = 375/441 (85.03%), Postives = 413/441 (93.65%), Query Frame = 0

Query: 1   MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 60
           MQSS DLDL+S L+ELIPEQQDRLKK K+EHGKVQLGNITVDMV+GGMRGMTGLLWETSL
Sbjct: 33  MQSSTDLDLKSQLQELIPEQQDRLKKLKSEHGKVQLGNITVDMVIGGMRGMTGLLWETSL 92

Query: 61  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 120
           LDP+EGIRFRGLSIPECQK+LP A+   EPLPEGLLWLLLTGKVPSKEQV+ALSK+L +R
Sbjct: 93  LDPEEGIRFRGLSIPECQKVLPTAQSGAEPLPEGLLWLLLTGKVPSKEQVEALSKDLANR 152

Query: 121 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN 180
           A VPDYVY AIDALP T+HPMTQFA+GVM LQVQSEF KAYE GIHKSK+WEPTYED +N
Sbjct: 153 AAVPDYVYNAIDALPSTAHPMTQFASGVMALQVQSEFQKAYENGIHKSKFWEPTYEDCLN 212

Query: 181 LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 240
           L+ARVP+VA+YVYRR+YK+G  IP D SLDYG NFSHMLGFD  +++ELMRLY+TIH+DH
Sbjct: 213 LIARVPVVAAYVYRRMYKNGDSIPSDKSLDYGANFSHMLGFDDEKVKELMRLYITIHSDH 272

Query: 241 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 300
           EGGNVSAHTGHLV SALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVV+ECGE+I+K
Sbjct: 273 EGGNVSAHTGHLVGSALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVEECGEDISK 332

Query: 301 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 360
           EQLK+YVWKTLNSGKV+PG+GHGVLR TDPRY CQREFALKHLPDDPLFQLVSKLYEVVP
Sbjct: 333 EQLKEYVWKTLNSGKVIPGYGHGVLRNTDPRYVCQREFALKHLPDDPLFQLVSKLYEVVP 392

Query: 361 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 420
           P+LTELGKVKNPWPNVDAHSGVLLN++GLTEARY+TVLFGVSRS+GICSQLIWDRALGL 
Sbjct: 393 PVLTELGKVKNPWPNVDAHSGVLLNHYGLTEARYYTVLFGVSRSLGICSQLIWDRALGLA 452

Query: 421 LERPKSVTLKWLEDHCKKAAA 442
           LERPKSVT+ WLE HCKKA++
Sbjct: 453 LERPKSVTMDWLEAHCKKASS 473

BLAST of Csor.00g004540 vs. ExPASy Swiss-Prot
Match: Q9M1D3 (Citrate synthase 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CSY5 PE=1 SV=2)

HSP 1 Score: 755.4 bits (1949), Expect = 3.7e-217
Identity = 364/432 (84.26%), Postives = 398/432 (92.13%), Query Frame = 0

Query: 4   SPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSLLDP 63
           S +LDL+S ++E+IPEQQDRLKK K+E GKV +GNITVDMVLGGMRGMTGLLWETSLLD 
Sbjct: 32  SSELDLKSQMQEIIPEQQDRLKKLKSEQGKVPVGNITVDMVLGGMRGMTGLLWETSLLDA 91

Query: 64  DEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSRAVV 123
           DEGIRFRG+SIPECQK+LP+A+   EPLPE LLWLLLTGKVP+KEQ +ALS EL  RA V
Sbjct: 92  DEGIRFRGMSIPECQKILPSAESGEEPLPESLLWLLLTGKVPTKEQANALSTELAHRAAV 151

Query: 124 PDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKG-IHKSKYWEPTYEDSMNLV 183
           P     AIDALP T+HPMTQFA+GVM LQVQSEF KAYE+G I KSKYWEPT+ED++NL+
Sbjct: 152 P-----AIDALPSTAHPMTQFASGVMALQVQSEFQKAYEQGDISKSKYWEPTFEDALNLI 211

Query: 184 ARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDHEG 243
           ARVP+VASYVYRR+YKDG IIP DDSLDYG NFSHMLGFDSPQM+ELMRLYVTIH+DHEG
Sbjct: 212 ARVPVVASYVYRRMYKDGSIIPLDDSLDYGANFSHMLGFDSPQMKELMRLYVTIHSDHEG 271

Query: 244 GNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITKEQ 303
           GNVSAH GHLV SALSDPYLSFAAALNGLAGPLHGLANQEVLLWIK VV+ECGE+I+KEQ
Sbjct: 272 GNVSAHAGHLVGSALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKLVVEECGESISKEQ 331

Query: 304 LKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVPPI 363
           LKDYVWKTLNSGKVVPG+GHGVLRKTDPRY CQREFALKHLPDDPLFQLVSKLYEVVPPI
Sbjct: 332 LKDYVWKTLNSGKVVPGYGHGVLRKTDPRYICQREFALKHLPDDPLFQLVSKLYEVVPPI 391

Query: 364 LTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLPLE 423
           LTELGKVKNPWPNVDAHSGVLLNY+GLTEARY+TVLFGVSRS+GICSQLIWDRALGLPLE
Sbjct: 392 LTELGKVKNPWPNVDAHSGVLLNYYGLTEARYYTVLFGVSRSLGICSQLIWDRALGLPLE 451

Query: 424 RPKSVTLKWLED 435
           RPKSV + WL++
Sbjct: 452 RPKSVNMDWLDN 458

BLAST of Csor.00g004540 vs. ExPASy Swiss-Prot
Match: P83372 (Citrate synthase, mitochondrial OS=Fragaria ananassa OX=3747 GN=MCSI PE=1 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 1.9e-213
Identity = 367/446 (82.29%), Postives = 397/446 (89.01%), Query Frame = 0

Query: 2   QSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSLL 61
           Q+S DLDL S L+ELIPEQQ+RLKK K EHGKVQLG ITVDMV+GGMRGMTGLLWETSLL
Sbjct: 30  QASSDLDLHSQLKELIPEQQERLKKLKKEHGKVQLGTITVDMVIGGMRGMTGLLWETSLL 89

Query: 62  DPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQ--- 121
           DPDEGIRFRGLSIPECQK+LP A P GEPLPEGLLWLLLTGKVPS+      SK +    
Sbjct: 90  DPDEGIRFRGLSIPECQKVLPGATPGGEPLPEGLLWLLLTGKVPSR------SKNMHYPV 149

Query: 122 SRAVVPDYVYKAIDALP--VTSH-PMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTY 181
           +  VVP +       L   +  H PMTQF TGVM LQVQSEF KAY+KGI KS+YWEPTY
Sbjct: 150 NNGVVPKFQIMCSRPLMLCLLEHIPMTQFTTGVMALQVQSEFQKAYDKGIPKSRYWEPTY 209

Query: 182 EDSMNLVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVT 241
           EDS++L+A++P+VASYVYRRIYK G +IP DDSLDYGGNFSH+LGFD  +MQELMRLYVT
Sbjct: 210 EDSLSLIAQLPVVASYVYRRIYKGGRMIPVDDSLDYGGNFSHLLGFDDHKMQELMRLYVT 269

Query: 242 IHTDHEGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECG 301
           IH+DHEGGNVSAHTGHLVASALSDP+LSFAAALNGLAGPLHGLANQEVLLWIKSVVDECG
Sbjct: 270 IHSDHEGGNVSAHTGHLVASALSDPFLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECG 329

Query: 302 ENITKEQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKL 361
           ENITK+QLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLF+LVSKL
Sbjct: 330 ENITKDQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFRLVSKL 389

Query: 362 YEVVPPILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDR 421
           Y+VVPPILTELGKVKNPWPNVDAHSGVLLN+FGLTEARYFTVLFGVSRSIGI SQLIWDR
Sbjct: 390 YDVVPPILTELGKVKNPWPNVDAHSGVLLNHFGLTEARYFTVLFGVSRSIGIGSQLIWDR 449

Query: 422 ALGLPLERPKSVTLKWLEDHCKKAAA 442
           ALGLPLERPKSVT++ LE  CKKAA+
Sbjct: 450 ALGLPLERPKSVTMESLESFCKKAAS 469

BLAST of Csor.00g004540 vs. NCBI nr
Match: KAG6593337.1 (Citrate synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 1   MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 60
           MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL
Sbjct: 1   MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 60

Query: 61  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 120
           LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR
Sbjct: 61  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 120

Query: 121 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN 180
           AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN
Sbjct: 121 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN 180

Query: 181 LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 240
           LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH
Sbjct: 181 LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 240

Query: 241 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 300
           EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK
Sbjct: 241 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 300

Query: 301 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 360
           EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP
Sbjct: 301 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 360

Query: 361 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 420
           PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP
Sbjct: 361 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 420

Query: 421 LERPKSVTLKWLEDHCKKAAA 441
           LERPKSVTLKWLEDHCKKAAA
Sbjct: 421 LERPKSVTLKWLEDHCKKAAA 441

BLAST of Csor.00g004540 vs. NCBI nr
Match: KAG7025685.1 (Citrate synthase, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 899 bits (2323), Expect = 0.0
Identity = 439/441 (99.55%), Postives = 440/441 (99.77%), Query Frame = 0

Query: 1   MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 60
           MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL
Sbjct: 32  MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 91

Query: 61  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 120
           LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR
Sbjct: 92  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 151

Query: 121 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN 180
           AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEF KAYEKGIHKSKYWEPTYEDSMN
Sbjct: 152 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFQKAYEKGIHKSKYWEPTYEDSMN 211

Query: 181 LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 240
           L+ARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH
Sbjct: 212 LIARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 271

Query: 241 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 300
           EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK
Sbjct: 272 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 331

Query: 301 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 360
           EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP
Sbjct: 332 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 391

Query: 361 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 420
           PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP
Sbjct: 392 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 451

Query: 421 LERPKSVTLKWLEDHCKKAAA 441
           LERPKSVTLKWLEDHCKKAAA
Sbjct: 452 LERPKSVTLKWLEDHCKKAAA 472

BLAST of Csor.00g004540 vs. NCBI nr
Match: XP_022959686.1 (citrate synthase, mitochondrial [Cucurbita moschata])

HSP 1 Score: 899 bits (2323), Expect = 0.0
Identity = 439/441 (99.55%), Postives = 440/441 (99.77%), Query Frame = 0

Query: 1   MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 60
           MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL
Sbjct: 32  MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 91

Query: 61  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 120
           LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR
Sbjct: 92  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 151

Query: 121 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN 180
           AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEF KAYEKGIHKSKYWEPTYEDSMN
Sbjct: 152 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFQKAYEKGIHKSKYWEPTYEDSMN 211

Query: 181 LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 240
           L+ARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH
Sbjct: 212 LIARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 271

Query: 241 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 300
           EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK
Sbjct: 272 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 331

Query: 301 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 360
           EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP
Sbjct: 332 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 391

Query: 361 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 420
           PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP
Sbjct: 392 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 451

Query: 421 LERPKSVTLKWLEDHCKKAAA 441
           LERPKSVTLKWLEDHCKKAAA
Sbjct: 452 LERPKSVTLKWLEDHCKKAAA 472

BLAST of Csor.00g004540 vs. NCBI nr
Match: XP_023004717.1 (citrate synthase, mitochondrial [Cucurbita maxima])

HSP 1 Score: 899 bits (2323), Expect = 0.0
Identity = 439/441 (99.55%), Postives = 440/441 (99.77%), Query Frame = 0

Query: 1   MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 60
           MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL
Sbjct: 32  MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 91

Query: 61  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 120
           LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR
Sbjct: 92  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 151

Query: 121 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN 180
           AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEF KAYEKGIHKSKYWEPTYEDSMN
Sbjct: 152 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFQKAYEKGIHKSKYWEPTYEDSMN 211

Query: 181 LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 240
           L+ARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH
Sbjct: 212 LIARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 271

Query: 241 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 300
           EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK
Sbjct: 272 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 331

Query: 301 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 360
           EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP
Sbjct: 332 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 391

Query: 361 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 420
           PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP
Sbjct: 392 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 451

Query: 421 LERPKSVTLKWLEDHCKKAAA 441
           LERPKSVTLKWLEDHCKKAAA
Sbjct: 452 LERPKSVTLKWLEDHCKKAAA 472

BLAST of Csor.00g004540 vs. NCBI nr
Match: XP_023548934.1 (citrate synthase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 887 bits (2292), Expect = 0.0
Identity = 431/440 (97.95%), Postives = 438/440 (99.55%), Query Frame = 0

Query: 1   MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 60
           MQSS DLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL
Sbjct: 32  MQSSSDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 91

Query: 61  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 120
           LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVP+KEQVDALSKELQSR
Sbjct: 92  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPNKEQVDALSKELQSR 151

Query: 121 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN 180
           A+VPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEF KAYEKGIHKSKYWEPTYEDS+N
Sbjct: 152 AIVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFQKAYEKGIHKSKYWEPTYEDSLN 211

Query: 181 LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 240
           L+ARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIH+DH
Sbjct: 212 LIARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHSDH 271

Query: 241 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 300
           EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK
Sbjct: 272 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 331

Query: 301 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 360
           EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP
Sbjct: 332 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 391

Query: 361 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 420
           PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRS+GICSQLIWDRALGLP
Sbjct: 392 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSLGICSQLIWDRALGLP 451

Query: 421 LERPKSVTLKWLEDHCKKAA 440
           LERPKSVTLKWLED+CKKAA
Sbjct: 452 LERPKSVTLKWLEDYCKKAA 471

BLAST of Csor.00g004540 vs. ExPASy TrEMBL
Match: A0A6J1KR80 (Citrate synthase OS=Cucurbita maxima OX=3661 GN=LOC111497935 PE=3 SV=1)

HSP 1 Score: 899 bits (2323), Expect = 0.0
Identity = 439/441 (99.55%), Postives = 440/441 (99.77%), Query Frame = 0

Query: 1   MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 60
           MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL
Sbjct: 32  MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 91

Query: 61  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 120
           LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR
Sbjct: 92  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 151

Query: 121 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN 180
           AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEF KAYEKGIHKSKYWEPTYEDSMN
Sbjct: 152 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFQKAYEKGIHKSKYWEPTYEDSMN 211

Query: 181 LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 240
           L+ARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH
Sbjct: 212 LIARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 271

Query: 241 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 300
           EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK
Sbjct: 272 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 331

Query: 301 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 360
           EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP
Sbjct: 332 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 391

Query: 361 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 420
           PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP
Sbjct: 392 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 451

Query: 421 LERPKSVTLKWLEDHCKKAAA 441
           LERPKSVTLKWLEDHCKKAAA
Sbjct: 452 LERPKSVTLKWLEDHCKKAAA 472

BLAST of Csor.00g004540 vs. ExPASy TrEMBL
Match: A0A6J1H5J9 (Citrate synthase OS=Cucurbita moschata OX=3662 GN=LOC111460686 PE=3 SV=1)

HSP 1 Score: 899 bits (2323), Expect = 0.0
Identity = 439/441 (99.55%), Postives = 440/441 (99.77%), Query Frame = 0

Query: 1   MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 60
           MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL
Sbjct: 32  MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 91

Query: 61  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 120
           LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR
Sbjct: 92  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 151

Query: 121 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN 180
           AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEF KAYEKGIHKSKYWEPTYEDSMN
Sbjct: 152 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFQKAYEKGIHKSKYWEPTYEDSMN 211

Query: 181 LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 240
           L+ARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH
Sbjct: 212 LIARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 271

Query: 241 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 300
           EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK
Sbjct: 272 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 331

Query: 301 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 360
           EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP
Sbjct: 332 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 391

Query: 361 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 420
           PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP
Sbjct: 392 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 451

Query: 421 LERPKSVTLKWLEDHCKKAAA 441
           LERPKSVTLKWLEDHCKKAAA
Sbjct: 452 LERPKSVTLKWLEDHCKKAAA 472

BLAST of Csor.00g004540 vs. ExPASy TrEMBL
Match: A0A6J1GNA7 (Citrate synthase OS=Cucurbita moschata OX=3662 GN=LOC111455982 PE=3 SV=1)

HSP 1 Score: 885 bits (2287), Expect = 0.0
Identity = 430/440 (97.73%), Postives = 437/440 (99.32%), Query Frame = 0

Query: 1   MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 60
           MQSS DLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL
Sbjct: 32  MQSSSDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 91

Query: 61  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 120
           LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVP+KEQVDALSKELQSR
Sbjct: 92  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPNKEQVDALSKELQSR 151

Query: 121 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN 180
           A+VPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEF KAYEKGIHKSKYWEPTYEDS+N
Sbjct: 152 AIVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFQKAYEKGIHKSKYWEPTYEDSLN 211

Query: 181 LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 240
           L+ARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIH+DH
Sbjct: 212 LIARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHSDH 271

Query: 241 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 300
           EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK
Sbjct: 272 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 331

Query: 301 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 360
           EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVS LYEVVP
Sbjct: 332 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSNLYEVVP 391

Query: 361 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 420
           PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRS+GICSQLIWDRALGLP
Sbjct: 392 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSLGICSQLIWDRALGLP 451

Query: 421 LERPKSVTLKWLEDHCKKAA 440
           LERPKSVTLKWLED+CKKAA
Sbjct: 452 LERPKSVTLKWLEDYCKKAA 471

BLAST of Csor.00g004540 vs. ExPASy TrEMBL
Match: A0A6J1JN57 (Citrate synthase OS=Cucurbita maxima OX=3661 GN=LOC111488392 PE=3 SV=1)

HSP 1 Score: 884 bits (2283), Expect = 0.0
Identity = 429/440 (97.50%), Postives = 436/440 (99.09%), Query Frame = 0

Query: 1   MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 60
           MQSS DLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL
Sbjct: 32  MQSSSDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 91

Query: 61  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 120
           LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVP+KEQVDALSKELQSR
Sbjct: 92  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPNKEQVDALSKELQSR 151

Query: 121 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN 180
           A+VPDYVYKAID LPVTSHPMTQFATGVMGLQVQSEF KAYEKGIHKSKYWEPTYEDS+N
Sbjct: 152 AIVPDYVYKAIDVLPVTSHPMTQFATGVMGLQVQSEFQKAYEKGIHKSKYWEPTYEDSLN 211

Query: 181 LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 240
           L+ARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIH+DH
Sbjct: 212 LIARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHSDH 271

Query: 241 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 300
           EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK
Sbjct: 272 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 331

Query: 301 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 360
           EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVS LYEVVP
Sbjct: 332 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSNLYEVVP 391

Query: 361 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 420
           PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRS+GICSQLIWDRALGLP
Sbjct: 392 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSLGICSQLIWDRALGLP 451

Query: 421 LERPKSVTLKWLEDHCKKAA 440
           LERPKSVTLKWLED+CKKAA
Sbjct: 452 LERPKSVTLKWLEDYCKKAA 471

BLAST of Csor.00g004540 vs. ExPASy TrEMBL
Match: A0A0A0K6P2 (Citrate synthase OS=Cucumis sativus OX=3659 GN=Csa_7G429590 PE=3 SV=1)

HSP 1 Score: 877 bits (2267), Expect = 0.0
Identity = 424/439 (96.58%), Postives = 436/439 (99.32%), Query Frame = 0

Query: 1   MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 60
           MQSS DLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL
Sbjct: 32  MQSSSDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 91

Query: 61  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 120
           LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALS+ELQSR
Sbjct: 92  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSRELQSR 151

Query: 121 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN 180
           A VPDYVYKAIDALP+TSHPMTQFATGVMGLQVQSEF KAYEKGIHKSKYWEPTYEDS+N
Sbjct: 152 ATVPDYVYKAIDALPITSHPMTQFATGVMGLQVQSEFQKAYEKGIHKSKYWEPTYEDSLN 211

Query: 181 LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 240
           L+A+VPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIH+DH
Sbjct: 212 LIAQVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHSDH 271

Query: 241 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 300
           EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVV+ECGENITK
Sbjct: 272 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVEECGENITK 331

Query: 301 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 360
           +QLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP
Sbjct: 332 DQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 391

Query: 361 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 420
           PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRS+GICSQLIWDRALGLP
Sbjct: 392 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSLGICSQLIWDRALGLP 451

Query: 421 LERPKSVTLKWLEDHCKKA 439
           LERPKSVT++WLED+CKKA
Sbjct: 452 LERPKSVTMQWLEDYCKKA 470

BLAST of Csor.00g004540 vs. TAIR 10
Match: AT2G44350.1 (Citrate synthase family protein )

HSP 1 Score: 795.8 bits (2054), Expect = 1.8e-230
Identity = 375/441 (85.03%), Postives = 413/441 (93.65%), Query Frame = 0

Query: 1   MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 60
           MQSS DLDL+S L+ELIPEQQDRLKK K+EHGKVQLGNITVDMV+GGMRGMTGLLWETSL
Sbjct: 32  MQSSTDLDLKSQLQELIPEQQDRLKKLKSEHGKVQLGNITVDMVIGGMRGMTGLLWETSL 91

Query: 61  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 120
           LDP+EGIRFRGLSIPECQK+LP A+   EPLPEGLLWLLLTGKVPSKEQV+ALSK+L +R
Sbjct: 92  LDPEEGIRFRGLSIPECQKVLPTAQSGAEPLPEGLLWLLLTGKVPSKEQVEALSKDLANR 151

Query: 121 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN 180
           A VPDYVY AIDALP T+HPMTQFA+GVM LQVQSEF KAYE GIHKSK+WEPTYED +N
Sbjct: 152 AAVPDYVYNAIDALPSTAHPMTQFASGVMALQVQSEFQKAYENGIHKSKFWEPTYEDCLN 211

Query: 181 LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 240
           L+ARVP+VA+YVYRR+YK+G  IP D SLDYG NFSHMLGFD  +++ELMRLY+TIH+DH
Sbjct: 212 LIARVPVVAAYVYRRMYKNGDSIPSDKSLDYGANFSHMLGFDDEKVKELMRLYITIHSDH 271

Query: 241 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 300
           EGGNVSAHTGHLV SALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVV+ECGE+I+K
Sbjct: 272 EGGNVSAHTGHLVGSALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVEECGEDISK 331

Query: 301 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 360
           EQLK+YVWKTLNSGKV+PG+GHGVLR TDPRY CQREFALKHLPDDPLFQLVSKLYEVVP
Sbjct: 332 EQLKEYVWKTLNSGKVIPGYGHGVLRNTDPRYVCQREFALKHLPDDPLFQLVSKLYEVVP 391

Query: 361 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 420
           P+LTELGKVKNPWPNVDAHSGVLLN++GLTEARY+TVLFGVSRS+GICSQLIWDRALGL 
Sbjct: 392 PVLTELGKVKNPWPNVDAHSGVLLNHYGLTEARYYTVLFGVSRSLGICSQLIWDRALGLA 451

Query: 421 LERPKSVTLKWLEDHCKKAAA 442
           LERPKSVT+ WLE HCKKA++
Sbjct: 452 LERPKSVTMDWLEAHCKKASS 472

BLAST of Csor.00g004540 vs. TAIR 10
Match: AT2G44350.2 (Citrate synthase family protein )

HSP 1 Score: 795.8 bits (2054), Expect = 1.8e-230
Identity = 375/441 (85.03%), Postives = 413/441 (93.65%), Query Frame = 0

Query: 1   MQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSL 60
           MQSS DLDL+S L+ELIPEQQDRLKK K+EHGKVQLGNITVDMV+GGMRGMTGLLWETSL
Sbjct: 33  MQSSTDLDLKSQLQELIPEQQDRLKKLKSEHGKVQLGNITVDMVIGGMRGMTGLLWETSL 92

Query: 61  LDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSR 120
           LDP+EGIRFRGLSIPECQK+LP A+   EPLPEGLLWLLLTGKVPSKEQV+ALSK+L +R
Sbjct: 93  LDPEEGIRFRGLSIPECQKVLPTAQSGAEPLPEGLLWLLLTGKVPSKEQVEALSKDLANR 152

Query: 121 AVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKGIHKSKYWEPTYEDSMN 180
           A VPDYVY AIDALP T+HPMTQFA+GVM LQVQSEF KAYE GIHKSK+WEPTYED +N
Sbjct: 153 AAVPDYVYNAIDALPSTAHPMTQFASGVMALQVQSEFQKAYENGIHKSKFWEPTYEDCLN 212

Query: 181 LVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDH 240
           L+ARVP+VA+YVYRR+YK+G  IP D SLDYG NFSHMLGFD  +++ELMRLY+TIH+DH
Sbjct: 213 LIARVPVVAAYVYRRMYKNGDSIPSDKSLDYGANFSHMLGFDDEKVKELMRLYITIHSDH 272

Query: 241 EGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITK 300
           EGGNVSAHTGHLV SALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVV+ECGE+I+K
Sbjct: 273 EGGNVSAHTGHLVGSALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVEECGEDISK 332

Query: 301 EQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVP 360
           EQLK+YVWKTLNSGKV+PG+GHGVLR TDPRY CQREFALKHLPDDPLFQLVSKLYEVVP
Sbjct: 333 EQLKEYVWKTLNSGKVIPGYGHGVLRNTDPRYVCQREFALKHLPDDPLFQLVSKLYEVVP 392

Query: 361 PILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLP 420
           P+LTELGKVKNPWPNVDAHSGVLLN++GLTEARY+TVLFGVSRS+GICSQLIWDRALGL 
Sbjct: 393 PVLTELGKVKNPWPNVDAHSGVLLNHYGLTEARYYTVLFGVSRSLGICSQLIWDRALGLA 452

Query: 421 LERPKSVTLKWLEDHCKKAAA 442
           LERPKSVT+ WLE HCKKA++
Sbjct: 453 LERPKSVTMDWLEAHCKKASS 473

BLAST of Csor.00g004540 vs. TAIR 10
Match: AT3G60100.1 (citrate synthase 5 )

HSP 1 Score: 755.4 bits (1949), Expect = 2.6e-218
Identity = 364/432 (84.26%), Postives = 398/432 (92.13%), Query Frame = 0

Query: 4   SPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSLLDP 63
           S +LDL+S ++E+IPEQQDRLKK K+E GKV +GNITVDMVLGGMRGMTGLLWETSLLD 
Sbjct: 32  SSELDLKSQMQEIIPEQQDRLKKLKSEQGKVPVGNITVDMVLGGMRGMTGLLWETSLLDA 91

Query: 64  DEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSRAVV 123
           DEGIRFRG+SIPECQK+LP+A+   EPLPE LLWLLLTGKVP+KEQ +ALS EL  RA V
Sbjct: 92  DEGIRFRGMSIPECQKILPSAESGEEPLPESLLWLLLTGKVPTKEQANALSTELAHRAAV 151

Query: 124 PDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFPKAYEKG-IHKSKYWEPTYEDSMNLV 183
           P     AIDALP T+HPMTQFA+GVM LQVQSEF KAYE+G I KSKYWEPT+ED++NL+
Sbjct: 152 P-----AIDALPSTAHPMTQFASGVMALQVQSEFQKAYEQGDISKSKYWEPTFEDALNLI 211

Query: 184 ARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDHEG 243
           ARVP+VASYVYRR+YKDG IIP DDSLDYG NFSHMLGFDSPQM+ELMRLYVTIH+DHEG
Sbjct: 212 ARVPVVASYVYRRMYKDGSIIPLDDSLDYGANFSHMLGFDSPQMKELMRLYVTIHSDHEG 271

Query: 244 GNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITKEQ 303
           GNVSAH GHLV SALSDPYLSFAAALNGLAGPLHGLANQEVLLWIK VV+ECGE+I+KEQ
Sbjct: 272 GNVSAHAGHLVGSALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKLVVEECGESISKEQ 331

Query: 304 LKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVSKLYEVVPPI 363
           LKDYVWKTLNSGKVVPG+GHGVLRKTDPRY CQREFALKHLPDDPLFQLVSKLYEVVPPI
Sbjct: 332 LKDYVWKTLNSGKVVPGYGHGVLRKTDPRYICQREFALKHLPDDPLFQLVSKLYEVVPPI 391

Query: 364 LTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLPLE 423
           LTELGKVKNPWPNVDAHSGVLLNY+GLTEARY+TVLFGVSRS+GICSQLIWDRALGLPLE
Sbjct: 392 LTELGKVKNPWPNVDAHSGVLLNYYGLTEARYYTVLFGVSRSLGICSQLIWDRALGLPLE 451

Query: 424 RPKSVTLKWLED 435
           RPKSV + WL++
Sbjct: 452 RPKSVNMDWLDN 458

BLAST of Csor.00g004540 vs. TAIR 10
Match: AT2G42790.1 (citrate synthase 3 )

HSP 1 Score: 128.3 bits (321), Expect = 1.6e-29
Identity = 112/398 (28.14%), Postives = 186/398 (46.73%), Query Frame = 0

Query: 59  SLLDPDEGI-RFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKEL 118
           S +D DEGI R+RG  I E  +     +         + +LL+ G +PS+ Q+      +
Sbjct: 105 SYIDGDEGILRYRGYPIEEMAENSTFLE---------VAYLLMYGNLPSESQLSDWEFAV 164

Query: 119 QSRAVVPDYVYKAIDALPVTSHPMTQFATGVMGLQV--QSEFPKAYEKGIHKSKYWEPTY 178
              + VP  V   I ++P  +HPM    + +  L +      P    + I+ SK  +   
Sbjct: 165 SQHSAVPQGVLDIIQSMPHDAHPMGVLVSAMSALSIFHPDANPALRGQDIYDSK--QVRD 224

Query: 179 EDSMNLVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGF-------DSPQMQE 238
           +  + ++ + P +A+  Y R+     ++P   +L Y  NF +ML          +P++  
Sbjct: 225 KQIIRIIGKAPTIAAAAYLRMAGRPPVLP-SGNLPYADNFLYMLDSLGNRSYKPNPRLAR 284

Query: 239 LMRLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIK 298
           ++ +   +H +HE    +A   HL +S + D Y + A A+  L GPLHG AN+ VL    
Sbjct: 285 VLDILFILHAEHEMNCSTAAARHLASSGV-DVYTAVAGAVGALYGPLHGGANEAVL---- 344

Query: 299 SVVDECGENITKEQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPL 358
            ++ E G   T E + +++    N  + + GFGH V +  DPR        +K+L D+ +
Sbjct: 345 KMLSEIG---TVENIPEFIEGVKNRKRKMSGFGHRVYKNYDPRAK-----VIKNLADE-V 404

Query: 359 FQLVSK--LYEVVPPI----LTELGKVKNP-WPNVDAHSGVLLNYFGLTEARYFTVLFGV 418
           F +V K  L EV   +    L++   VK   +PNVD +SG++    G     +FTVLF +
Sbjct: 405 FSIVGKDPLIEVAVALEKAALSDDYFVKRKLYPNVDFYSGLIYRAMGF-PPEFFTVLFAI 464

Query: 419 SRSIGICSQLIWDRALGLP---LERPKSV-TLKWLEDH 436
            R  G  S   W  +L  P   + RP+ V T  WL  +
Sbjct: 465 PRMAGYLSH--WKESLDDPDTKIMRPQQVYTGVWLRHY 473

BLAST of Csor.00g004540 vs. TAIR 10
Match: AT3G58750.1 (citrate synthase 2 )

HSP 1 Score: 125.6 bits (314), Expect = 1.0e-28
Identity = 102/373 (27.35%), Postives = 170/373 (45.58%), Query Frame = 0

Query: 61  LDPDEGI-RFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQS 120
           +D DEGI R+RG  I E  +     +         + +LL+ G +PS+ Q+      +  
Sbjct: 112 IDGDEGILRYRGYPIEELAESSTFIE---------VAYLLMYGNLPSQSQLADWEFTVSQ 171

Query: 121 RAVVPDYVYKAIDALPVTSHPMTQFATGVMGLQV--QSEFPKAYEKGIHKSKYWEPTYED 180
            + VP  V   I ++P  +HPM    + +  L +      P    + I+KSK  +   + 
Sbjct: 172 HSAVPQGVLDIIQSMPHDAHPMGVLVSAMSALSIFHPDANPALSGQDIYKSK--QVRDKQ 231

Query: 181 SMNLVARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGF-------DSPQMQELM 240
            + ++ + P +A+  Y R      ++P   +L Y  NF +ML          +P++  ++
Sbjct: 232 IVRILGKAPTIAAAAYLRTAGRPPVLP-SANLSYSENFLYMLDSMGNRSYKPNPRLARVL 291

Query: 241 RLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSV 300
            +   +H +HE    +A   HL +S + D Y + A A+  L GPLHG AN+ VL     +
Sbjct: 292 DILFILHAEHEMNCSTAAARHLASSGV-DVYTACAGAVGALYGPLHGGANEAVL----KM 351

Query: 301 VDECGENITKEQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFA---LKHLPDDP 360
           + E G   T E + D++    N  + + GFGH V +  DPR    ++ A      +  DP
Sbjct: 352 LAEIG---TAENIPDFIEGVKNRKRKMSGFGHRVYKNYDPRAKVIKKLADEVFSIVGRDP 411

Query: 361 LFQLVSKLYEVVPPILTELGKVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGI 420
           L ++   L +    +  E    +  +PNVD +SG++    G     +FTVLF V R  G 
Sbjct: 412 LIEVAVALEKAA--LSDEYFVKRKLYPNVDFYSGLIYRAMGF-PPEFFTVLFAVPRMAGY 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P492982.5e-23789.09Citrate synthase, mitochondrial OS=Citrus maxima OX=37334 GN=CIT PE=2 SV=1[more]
O804337.4e-23486.76Citrate synthase, mitochondrial OS=Daucus carota OX=4039 GN=CS PE=2 SV=1[more]
P201152.5e-22985.03Citrate synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CSY4 PE=1 S... [more]
Q9M1D33.7e-21784.26Citrate synthase 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CSY5 PE=1 S... [more]
P833721.9e-21382.29Citrate synthase, mitochondrial OS=Fragaria ananassa OX=3747 GN=MCSI PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6593337.10.0100.00Citrate synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7025685.10.099.55Citrate synthase, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022959686.10.099.55citrate synthase, mitochondrial [Cucurbita moschata][more]
XP_023004717.10.099.55citrate synthase, mitochondrial [Cucurbita maxima][more]
XP_023548934.10.097.95citrate synthase, mitochondrial [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1KR800.099.55Citrate synthase OS=Cucurbita maxima OX=3661 GN=LOC111497935 PE=3 SV=1[more]
A0A6J1H5J90.099.55Citrate synthase OS=Cucurbita moschata OX=3662 GN=LOC111460686 PE=3 SV=1[more]
A0A6J1GNA70.097.73Citrate synthase OS=Cucurbita moschata OX=3662 GN=LOC111455982 PE=3 SV=1[more]
A0A6J1JN570.097.50Citrate synthase OS=Cucurbita maxima OX=3661 GN=LOC111488392 PE=3 SV=1[more]
A0A0A0K6P20.096.58Citrate synthase OS=Cucumis sativus OX=3659 GN=Csa_7G429590 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G44350.11.8e-23085.03Citrate synthase family protein [more]
AT2G44350.21.8e-23085.03Citrate synthase family protein [more]
AT3G60100.12.6e-21884.26citrate synthase 5 [more]
AT2G42790.11.6e-2928.14citrate synthase 3 [more]
AT3G58750.11.0e-2827.35citrate synthase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002020Citrate synthasePRINTSPR00143CITRTSNTHASEcoord: 312..332
score: 57.78
coord: 394..408
score: 53.72
coord: 252..280
score: 61.14
coord: 373..389
score: 55.43
coord: 181..194
score: 40.38
coord: 229..244
score: 47.24
IPR002020Citrate synthasePFAMPF00285Citrate_syntcoord: 47..425
e-value: 7.0E-101
score: 337.9
IPR002020Citrate synthasePANTHERPTHR11739CITRATE SYNTHASEcoord: 1..441
IPR016143Citrate synthase-like, small alpha subdomainGENE3D1.10.230.10coord: 279..393
e-value: 2.1E-193
score: 644.6
IPR010109Citrate synthase, eukaryotic-typeTIGRFAMTIGR01793TIGR01793coord: 7..432
e-value: 4.4E-246
score: 814.4
IPR016142Citrate synthase-like, large alpha subdomainGENE3D1.10.580.10Citrate Synthase, domain 1coord: 13..425
e-value: 2.1E-193
score: 644.6
NoneNo IPR availablePANTHERPTHR11739:SF33CITRATE SYNTHASE 4, MITOCHONDRIALcoord: 1..441
IPR019810Citrate synthase active sitePROSITEPS00480CITRATE_SYNTHASEcoord: 319..331
IPR036969Citrate synthase superfamilySUPERFAMILY48256Citrate synthasecoord: 11..436

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g004540.m01Csor.00g004540.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006101 citrate metabolic process
biological_process GO:0006099 tricarboxylic acid cycle
cellular_component GO:0005759 mitochondrial matrix
molecular_function GO:0004108 citrate (Si)-synthase activity
molecular_function GO:0046912 acyltransferase, acyl groups converted into alkyl on transfer