Csor.00g001870 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g001870
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	gibberellin 20-oxidase-like protein
Location	Csor_Chr17: 10374998 .. 10376073 (-)
RNA-Seq Expression	Csor.00g001870
Synteny	Csor.00g001870

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGATAAAAGAAGTTCCAGTGATAGACCTTAGCAGTCAGAGCTTGACTGAATCGACTGCATCTTCTCTACTTAGAGCGTGCAACGAATTGGGATTCTTCTTCATCAGAAACCATGGCGTTTCAAAGGAGTTTTACGAGAAACTCATCTCTGGCGCCGATGAGCTTTTTTCTTCCCCCGAGGAGACCAAGCAACAACTGAAAATGGGAGTTTCCTCGTACACCCCGAGATTTGTGGTGTCGCCTTTCTTTGAGAGCTTCAGGGTTTCGGGACCTAACTTCTCAACCTCTGCTGCTACGCATGGTTTCACTCAATCACTATTCGGCCACCATGCTACTGAATTCAGGTTGTCTTCTCCTTAGAAATGTTTGCTTTCATCGCTATTAAAATTTGCGCGATGAGCTTTATTTTATACGGTTGTTCTATCATTATCTTCTTTATTTAGACACTAATTTATGTTTGTATGTTCGAGATTATTTGCAGCAATCTTCTAGACGAATATGGAAGCAAAATGAAAGAAGTATCGAAAAGAGTAGTGAAGCTATTACTAAACATCTTGAGGGATAATATTGAAAACAAATTCTATGATCCCGAGTTTAGCAACTGCAATGGCTACCTAAGAATAAACGGCTTCACTCCTCTAGACATGAATGCCGAAGAAGAAGTTGAAGTGTTCAAGAAGCACACCGACCTCAGCTACGTGACGATAGTGTTCCCAAATGACATGAGGGGGCTACAAATGAGATCTAAAGGAGGAGATTGGATCGACATAAGGTCGAGCGAGGACGATATTCTTGTAGTGAACATTGGAGATTATATGGAGGCTTGGAGCAACGGGAGGCTGAGATCCGCCGTACATAGAGTTGTTTTAAGGAAAAATATTACGAAGAGATTCTCTATCGCTTTCTTATCGACACCGGAAGATGAATCTAGAGAAATACATGCACCGAGTGAAGTCGTTGGAGAAGGGAAATCAAGACTTTACCGCTCGTTTTCAATCCAACAATATAAACATTTTAGGGAAAACAATTACGAGAAAGTTGGAGCCACGATTAAGGATTTTGTTGGAATATGA

mRNA sequence

ATGTCGATAAAAGAAGTTCCAGTGATAGACCTTAGCAGTCAGAGCTTGACTGAATCGACTGCATCTTCTCTACTTAGAGCGTGCAACGAATTGGGATTCTTCTTCATCAGAAACCATGGCGTTTCAAAGGAGTTTTACGAGAAACTCATCTCTGGCGCCGATGAGCTTTTTTCTTCCCCCGAGGAGACCAAGCAACAACTGAAAATGGGAGTTTCCTCGTACACCCCGAGATTTGTGGTGTCGCCTTTCTTTGAGAGCTTCAGGGTTTCGGGACCTAACTTCTCAACCTCTGCTGCTACGCATGGTTTCACTCAATCACTATTCGGCCACCATGCTACTGAATTCAGCAATCTTCTAGACGAATATGGAAGCAAAATGAAAGAAGTATCGAAAAGAGTAGTGAAGCTATTACTAAACATCTTGAGGGATAATATTGAAAACAAATTCTATGATCCCGAGTTTAGCAACTGCAATGGCTACCTAAGAATAAACGGCTTCACTCCTCTAGACATGAATGCCGAAGAAGAAGTTGAAGTGTTCAAGAAGCACACCGACCTCAGCTACGTGACGATAGTGTTCCCAAATGACATGAGGGGGCTACAAATGAGATCTAAAGGAGGAGATTGGATCGACATAAGGTCGAGCGAGGACGATATTCTTGTAGTGAACATTGGAGATTATATGGAGGCTTGGAGCAACGGGAGGCTGAGATCCGCCGTACATAGAGTTGTTTTAAGGAAAAATATTACGAAGAGATTCTCTATCGCTTTCTTATCGACACCGGAAGATGAATCTAGAGAAATACATGCACCGAGTGAAGTCGTTGGAGAAGGGAAATCAAGACTTTACCGCTCGTTTTCAATCCAACAATATAAACATTTTAGGGAAAACAATTACGAGAAAGTTGGAGCCACGATTAAGGATTTTGTTGGAATATGA

Coding sequence (CDS)

ATGTCGATAAAAGAAGTTCCAGTGATAGACCTTAGCAGTCAGAGCTTGACTGAATCGACTGCATCTTCTCTACTTAGAGCGTGCAACGAATTGGGATTCTTCTTCATCAGAAACCATGGCGTTTCAAAGGAGTTTTACGAGAAACTCATCTCTGGCGCCGATGAGCTTTTTTCTTCCCCCGAGGAGACCAAGCAACAACTGAAAATGGGAGTTTCCTCGTACACCCCGAGATTTGTGGTGTCGCCTTTCTTTGAGAGCTTCAGGGTTTCGGGACCTAACTTCTCAACCTCTGCTGCTACGCATGGTTTCACTCAATCACTATTCGGCCACCATGCTACTGAATTCAGCAATCTTCTAGACGAATATGGAAGCAAAATGAAAGAAGTATCGAAAAGAGTAGTGAAGCTATTACTAAACATCTTGAGGGATAATATTGAAAACAAATTCTATGATCCCGAGTTTAGCAACTGCAATGGCTACCTAAGAATAAACGGCTTCACTCCTCTAGACATGAATGCCGAAGAAGAAGTTGAAGTGTTCAAGAAGCACACCGACCTCAGCTACGTGACGATAGTGTTCCCAAATGACATGAGGGGGCTACAAATGAGATCTAAAGGAGGAGATTGGATCGACATAAGGTCGAGCGAGGACGATATTCTTGTAGTGAACATTGGAGATTATATGGAGGCTTGGAGCAACGGGAGGCTGAGATCCGCCGTACATAGAGTTGTTTTAAGGAAAAATATTACGAAGAGATTCTCTATCGCTTTCTTATCGACACCGGAAGATGAATCTAGAGAAATACATGCACCGAGTGAAGTCGTTGGAGAAGGGAAATCAAGACTTTACCGCTCGTTTTCAATCCAACAATATAAACATTTTAGGGAAAACAATTACGAGAAAGTTGGAGCCACGATTAAGGATTTTGTTGGAATATGA

Protein sequence

MSIKEVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSPEETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSNLLDEYGSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVFKKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLRSAVHRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFRENNYEKVGATIKDFVGI

Homology

[Close]

BLAST of Csor.00g001870 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 5.5e-66
Identity = 145/326 (44.48%), Postives = 213/326 (65.34%), Query Frame = 0


Query: 5   EVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSPEETK 64
           E+PV D+ S+ L+ES+ +SL  AC E GFF++ NHGVS++ Y+KL   +  +F   +E K
Sbjct: 4   ELPVFDI-SKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEEK 63

Query: 65  QQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHAT--EFSNLLDEY 124
             +KMG S+YTPRF+ SPFFES RVSGP+F  SA +   +   F   AT  EFS L+ EY
Sbjct: 64  --MKMGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFSGLMKEY 123

Query: 125 GSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFT-PLDM-----NAEEE 184
           G KM ++ ++++K +L+   D++ +K+Y+ EF NC+GY RIN +T P D      N +E+
Sbjct: 124 GEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQ 183

Query: 185 --VEVFKKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNG 244
             +E    HTD+S +TIV  +D+ GLQ+R++ G  +   + +D+ LVVN+GD + AW+NG
Sbjct: 184 DLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLLHAWTNG 243

Query: 245 RLRSAVHRVVLRKN--ITKRFSIAFLSTPEDESREIHAPSEVVG--EGKSRLYRSFSIQQ 304
           RLRS+ HRV+L++   +  RFS+AF     D+ + + AP EVVG  EG  R++RSF    
Sbjct: 244 RLRSSQHRVILKRRGFVGNRFSLAFFWC-FDDGKVVFAPDEVVGGCEG-MRVFRSFKCGD 303

Query: 305 YKHFRENN----YEKVGATIKDFVGI 313
           Y  FRE+N    +EKVG T++DF  I
Sbjct: 304 YLRFRESNEKGKFEKVGDTVEDFARI 321

[Close]

BLAST of Csor.00g001870 vs. ExPASy Swiss-Prot
Match: Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 6.4e-22
Identity = 96/321 (29.91%), Postives = 149/321 (46.42%), Query Frame = 0


Query: 6   VPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSS------ 65
           VP IDLSSQ  T      +  AC + GFF + NHGVS    E LI+ A  L  S      
Sbjct: 63  VPFIDLSSQDSTLEAPRVIAEACTKHGFFLVVNHGVS----ESLIADAHRLMESFFDMPL 122

Query: 66  PEETKQQLKMG-----VSSYTPRFVVS-PFFE--SFRVSGPNFSTSAATHGFTQSLFGHH 125
             + K Q K G      SS+T RF    P+ E  SF+ S  N  +      F+ +L G  
Sbjct: 123 AGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDNSGSRTVQDYFSDTL-GQE 182

Query: 126 ATEFSNLLDEYGSKMKEVSKRVVKLL---LNILRDNIENKFYDPEFSNCNGYLRINGFTP 185
             +F  +  +Y   M  +S ++++LL   L + RD     ++   F   +  +R+N + P
Sbjct: 183 FEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRD-----YFRGFFEENDSIMRLNHYPP 242

Query: 186 LDMNAEEEVEVFKKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYM 245
                 +       H D S +TI+  + + GLQ+      W  IR +     VVNIGD  
Sbjct: 243 --CQTPDLTLGTGPHCDPSSLTILHQDHVNGLQV-FVDNQWQSIRPN-PKAFVVNIGDTF 302

Query: 246 EAWSNGRLRSAVHRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSI 305
            A SNG  +S +HR V+ +  + R S+AF   P+ + + +  PS+++ + K+R Y  F+ 
Sbjct: 303 MALSNGIFKSCLHRAVVNRE-SARKSMAFFLCPK-KDKVVKPPSDILEKMKTRKYPDFTW 362

Query: 306 QQYKHFRENNYEKVGATIKDF 310
             +  F + +Y     T+  F
Sbjct: 363 SMFLEFTQKHYRADVNTLDSF 367

[Close]

BLAST of Csor.00g001870 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.9e-21
Identity = 90/324 (27.78%), Postives = 149/324 (45.99%), Query Frame = 0


Query: 6   VPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSPEETKQ 65
           +P++DLS         + +++AC + GFF + NHG+  +   +L S A + FS P+  K+
Sbjct: 27  IPLVDLSK----PDAKTLIVKACEDFGFFKVINHGIPLDAISQLESEAFKFFSLPQTEKE 86

Query: 66  QLKMGVSSYTPRFVVSPFFESFRVSGPN---------FSTSAATHGFTQSLFGHHATEFS 125
             K G +        +PF    +  G N           T+   H F  SL+G    +F 
Sbjct: 87  --KAGPA--------NPFGYGNKRIGLNGDIGWIEYLLLTTNQDHNF--SLYGEDIDKFR 146

Query: 126 NLLDEYGSKMKEVSKRVVKLL---LNILRDNIENKFYDPEFSNCNGYLRINGF---TPLD 185
            LL +Y   M+ ++  ++ L+   L I   N+ +K    + S+C    R+N +     L 
Sbjct: 147 GLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDC--LFRVNHYPACPELA 206

Query: 186 MNAEEEVEVFKKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEA 245
           +N E  +  F +HTD   ++I+  N+  G Q+  + G WI +         +N+GD ++ 
Sbjct: 207 INGENLIG-FGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSSFF-INVGDSLQV 266

Query: 246 WSNGRLRSAVHRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQ 305
            +NGR +S  HR VL   I  R S+ +   P     E  AP   + +GK  LY+ F+  +
Sbjct: 267 MTNGRFKSVRHR-VLANGIDPRLSMIYFCGP--PLSEKIAPLPSLMKGKESLYKEFTWFE 326

[Close]

BLAST of Csor.00g001870 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 3.2e-21
Identity = 86/313 (27.48%), Postives = 149/313 (47.60%), Query Frame = 0


Query: 2   SIKEVPVIDLSSQSLTESTASSLL-RACNELGFFFIRNHGVSKEFYEKLISGADELFSSP 61
           S   VPVIDL+     +  AS L+  AC   G + + NHG+     E +      LFS P
Sbjct: 52  STTTVPVIDLN-----DPNASKLIGLACKTWGVYQVMNHGIPLSLLEDIQWLGQTLFSLP 111

Query: 62  EETKQQLKM---GVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSN 121
              K +      GVS Y    + S F +     G     S   H   + L+    T F +
Sbjct: 112 SHQKHKATRSPDGVSGYGIARISSFFPKLMWYEGFTIVGSPLDH--FRELWPQDYTRFCD 171

Query: 122 LLDEYGSKMKEVSKRVVKLLLN---ILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAE 181
           ++ +Y   MK+++  ++ L+L+   I +++I+      +F      L++N +        
Sbjct: 172 IVVQYDETMKKLAGTLMCLMLDSLGITKEDIKWAGSKAQFEKACAALQLNSYP--SCPDP 231

Query: 182 EEVEVFKKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNG 241
           +       HTD +++TI+  ND+ GLQ+  +G  WI +   +   LVVN+GD     SNG
Sbjct: 232 DHAMGLAPHTDSTFLTILSQNDISGLQVNREGSGWITVPPLQGG-LVVNVGDLFHILSNG 291

Query: 242 RLRSAVHRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHF 301
              S +HRV++ +   +RFS+A+L  P   + EI   ++++G  K  LYRS +  +Y   
Sbjct: 292 LYPSVLHRVLVNRT-RQRFSVAYLYGP-PSNVEICPHAKLIGPTKPPLYRSVTWNEYLGT 351

Query: 302 RENNYEKVGATIK 308
           +  ++ K  ++++
Sbjct: 352 KAKHFNKALSSVR 352

[Close]

BLAST of Csor.00g001870 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.6e-20
Identity = 80/298 (26.85%), Postives = 134/298 (44.97%), Query Frame = 0


Query: 6   VPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSPEETK- 65
           +PV++L+         + +++AC E GFF + NHGV  E   +L   A   F  P+  K 
Sbjct: 31  IPVVNLAD----PEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKN 90

Query: 66  -----QQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSNLL 125
                +    G     P   V         + P  S+       T ++F      F   +
Sbjct: 91  RAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPK-----TSAVFRQTPQIFRESV 150

Query: 126 DEYGSKMKEVSKRVVKLLLNIL----RDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEE 185
           +EY  ++KEVS +V++++   L    RD +     D +  +C   LR+N +   +  AE+
Sbjct: 151 EEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSC---LRLNHYPAAEEEAEK 210

Query: 186 EVEV-FKKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNG 245
            V+V F +HTD   ++++  N+  GLQ+  K G W+ +         +N+GD ++  +NG
Sbjct: 211 MVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFF-INVGDALQVMTNG 270

Query: 246 RLRSAVHRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYK 293
           R +S  HR VL      R S+ +   P    +    P  +V E    LY+ F+  QYK
Sbjct: 271 RFKSVKHR-VLADTRRSRISMIYFGGPPLSQKIAPLPC-LVPEQDDWLYKEFTWSQYK 313

[Close]

BLAST of Csor.00g001870 vs. NCBI nr
Match: KAG6576056.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 621 bits (1601), Expect = 3.23e-224
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0


Query: 1   MSIKEVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSP 60
           MSIKEVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSP
Sbjct: 1   MSIKEVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSP 60

Query: 61  EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSNLLD 120
           EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSNLLD
Sbjct: 61  EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSNLLD 120

Query: 121 EYGSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF 180
           EYGSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF
Sbjct: 121 EYGSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF 180

Query: 181 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLRSAV 240
           KKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLRSAV
Sbjct: 181 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLRSAV 240

Query: 241 HRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFRENNYE 300
           HRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFRENNYE
Sbjct: 241 HRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFRENNYE 300

Query: 301 KVGATIKDFVGI 312
           KVGATIKDFVGI
Sbjct: 301 KVGATIKDFVGI 312

[Close]

BLAST of Csor.00g001870 vs. NCBI nr
Match: XP_022953358.1 (gibberellin 20-oxidase-like protein [Cucurbita moschata])

HSP 1 Score: 598 bits (1542), Expect = 3.18e-215
Identity = 300/312 (96.15%), Postives = 303/312 (97.12%), Query Frame = 0


Query: 1   MSIKEVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSP 60
           MSIKEVPVIDLS QSLTESTASSLLR CNELGFFFIRNHGVSKEFYEKLISGADELFSSP
Sbjct: 1   MSIKEVPVIDLSCQSLTESTASSLLRTCNELGFFFIRNHGVSKEFYEKLISGADELFSSP 60

Query: 61  EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSNLLD 120
           EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFS S A  GFTQSLFGHHATEFSNLLD
Sbjct: 61  EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFSASTAAQGFTQSLFGHHATEFSNLLD 120

Query: 121 EYGSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF 180
           EYGSKMKEVSKRVVKLLLNIL DN  NKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF
Sbjct: 121 EYGSKMKEVSKRVVKLLLNILGDNFGNKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF 180

Query: 181 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLRSAV 240
           KKHTDLSYVTIVFPNDMRGLQMRSKGGDWID+RSSEDDILVVNIGDYMEAWSNGRLRSAV
Sbjct: 181 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDVRSSEDDILVVNIGDYMEAWSNGRLRSAV 240

Query: 241 HRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFRENNYE 300
           HRVVLRKNITKRFSIAFLSTPEDESREI+AP+EVVGEGKSRLYRSFSIQQYKHFRENNYE
Sbjct: 241 HRVVLRKNITKRFSIAFLSTPEDESREIYAPNEVVGEGKSRLYRSFSIQQYKHFRENNYE 300

Query: 301 KVGATIKDFVGI 312
           KVGATIKDFVGI
Sbjct: 301 KVGATIKDFVGI 312

[Close]

BLAST of Csor.00g001870 vs. NCBI nr
Match: XP_022991455.1 (gibberellin 20-oxidase-like protein [Cucurbita maxima])

HSP 1 Score: 586 bits (1511), Expect = 1.69e-210
Identity = 292/312 (93.59%), Postives = 301/312 (96.47%), Query Frame = 0


Query: 1   MSIKEVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSP 60
           MSIKEVPVIDL  QSLTEST+SSLLRACN+LGFFFIRNHGVSKEFYEKLISGADELFSSP
Sbjct: 1   MSIKEVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSP 60

Query: 61  EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSNLLD 120
           EETKQQLKMGVSSYTPRF+VSPFFESFRVSGPNFS SAA  GFTQSLFGHHATEFSNLLD
Sbjct: 61  EETKQQLKMGVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLD 120

Query: 121 EYGSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF 180
           EYG KMKEVSKRVVKLLLN+L DNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEE+EVF
Sbjct: 121 EYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEIEVF 180

Query: 181 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLRSAV 240
           KKHTDLSYVTIVF ND+RGLQMRSKGGDWID+R SED ILVVNIGDYMEAWSNGRLRSAV
Sbjct: 181 KKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNGRLRSAV 240

Query: 241 HRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFRENNYE 300
           HRVVLRKNI KRFSIAFLSTPEDESREI+APSEVVGEGKSRLYRSFS+QQYKHFRENNYE
Sbjct: 241 HRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENNYE 300

Query: 301 KVGATIKDFVGI 312
           KVGATIKDFVGI
Sbjct: 301 KVGATIKDFVGI 312

[Close]

BLAST of Csor.00g001870 vs. NCBI nr
Match: KAG6576054.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 556 bits (1432), Expect = 1.85e-198
Identity = 276/312 (88.46%), Postives = 292/312 (93.59%), Query Frame = 0


Query: 1   MSIKEVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSP 60
           MSI+EVPVID+SSQSLTESTASSL RAC ELGFFFI+NHG+SKEFY+KLISG D+LFSSP
Sbjct: 1   MSIEEVPVIDISSQSLTESTASSLTRACTELGFFFIKNHGISKEFYQKLISGIDQLFSSP 60

Query: 61  EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSNLLD 120
           EETKQ+LK GVSSYTPRFVVSPF ESF++SGP FS SA THGFTQS FGH ATEFSNLLD
Sbjct: 61  EETKQKLKQGVSSYTPRFVVSPFLESFKLSGPKFSASAVTHGFTQSQFGHQATEFSNLLD 120

Query: 121 EYGSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF 180
           EYGSKM EVSK+VVKLLL IL DNIENKFYDPE+SNC  YLRING+TPL MN EEEVEVF
Sbjct: 121 EYGSKMTEVSKKVVKLLLKILGDNIENKFYDPEYSNCTSYLRINGYTPLGMNDEEEVEVF 180

Query: 181 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLRSAV 240
           KKHTDLSYVTIVFPNDMRGLQMRSKGGDWI++R SED  LVVNIGDYMEAWSNGRLRSA+
Sbjct: 181 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWINVRPSEDGNLVVNIGDYMEAWSNGRLRSAL 240

Query: 241 HRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFRENNYE 300
           HRVVLRKNITKRFSIAFLSTPEDESREI+APSEVVGEGKSRLYRSFSIQQYKHFRENNYE
Sbjct: 241 HRVVLRKNITKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENNYE 300

Query: 301 KVGATIKDFVGI 312
           KVGATIKDFVGI
Sbjct: 301 KVGATIKDFVGI 312

[Close]

BLAST of Csor.00g001870 vs. NCBI nr
Match: XP_022953360.1 (gibberellin 20-oxidase-like protein [Cucurbita moschata])

HSP 1 Score: 548 bits (1412), Expect = 2.06e-195
Identity = 272/312 (87.18%), Postives = 289/312 (92.63%), Query Frame = 0


Query: 1   MSIKEVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSP 60
           MSI+EVPVID+SSQ+LTESTASSL RAC ELGFFFI+NHG+SKEFY+KLISG D+LFSSP
Sbjct: 1   MSIEEVPVIDISSQTLTESTASSLSRACTELGFFFIKNHGISKEFYQKLISGIDQLFSSP 60

Query: 61  EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSNLLD 120
           EETKQ+LK GVSSYTPRFVVSPF ESF++SGP FS SA  HGFTQS FGH ATEFSNLLD
Sbjct: 61  EETKQKLKQGVSSYTPRFVVSPFLESFKLSGPKFSASAVAHGFTQSQFGHQATEFSNLLD 120

Query: 121 EYGSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF 180
           EYGSKM EVSK+VVKLLL IL DNIENKFYDPEFSNC  YLRIN +TPL MN EEEVEVF
Sbjct: 121 EYGSKMTEVSKKVVKLLLKILGDNIENKFYDPEFSNCTSYLRINSYTPLGMNDEEEVEVF 180

Query: 181 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLRSAV 240
           KKHTDLSY TIVFPNDMRGLQMRSKGGDWI++R SED  LVVNIGDYMEAWSNGRLRSA+
Sbjct: 181 KKHTDLSYATIVFPNDMRGLQMRSKGGDWINVRPSEDGNLVVNIGDYMEAWSNGRLRSAL 240

Query: 241 HRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFRENNYE 300
           HRVVLRKNITKRFSIAFLSTPEDESREI+APSEVVGEGKSRLYRSFSIQQYKHFRENNY+
Sbjct: 241 HRVVLRKNITKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENNYK 300

Query: 301 KVGATIKDFVGI 312
           KVGATIKDFVGI
Sbjct: 301 KVGATIKDFVGI 312

[Close]

BLAST of Csor.00g001870 vs. ExPASy TrEMBL
Match: A0A6J1GN55 (gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111455931 PE=3 SV=1)

HSP 1 Score: 598 bits (1542), Expect = 1.54e-215
Identity = 300/312 (96.15%), Postives = 303/312 (97.12%), Query Frame = 0


Query: 1   MSIKEVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSP 60
           MSIKEVPVIDLS QSLTESTASSLLR CNELGFFFIRNHGVSKEFYEKLISGADELFSSP
Sbjct: 1   MSIKEVPVIDLSCQSLTESTASSLLRTCNELGFFFIRNHGVSKEFYEKLISGADELFSSP 60

Query: 61  EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSNLLD 120
           EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFS S A  GFTQSLFGHHATEFSNLLD
Sbjct: 61  EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFSASTAAQGFTQSLFGHHATEFSNLLD 120

Query: 121 EYGSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF 180
           EYGSKMKEVSKRVVKLLLNIL DN  NKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF
Sbjct: 121 EYGSKMKEVSKRVVKLLLNILGDNFGNKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF 180

Query: 181 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLRSAV 240
           KKHTDLSYVTIVFPNDMRGLQMRSKGGDWID+RSSEDDILVVNIGDYMEAWSNGRLRSAV
Sbjct: 181 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDVRSSEDDILVVNIGDYMEAWSNGRLRSAV 240

Query: 241 HRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFRENNYE 300
           HRVVLRKNITKRFSIAFLSTPEDESREI+AP+EVVGEGKSRLYRSFSIQQYKHFRENNYE
Sbjct: 241 HRVVLRKNITKRFSIAFLSTPEDESREIYAPNEVVGEGKSRLYRSFSIQQYKHFRENNYE 300

Query: 301 KVGATIKDFVGI 312
           KVGATIKDFVGI
Sbjct: 301 KVGATIKDFVGI 312

[Close]

BLAST of Csor.00g001870 vs. ExPASy TrEMBL
Match: A0A6J1JSZ7 (gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111488065 PE=3 SV=1)

HSP 1 Score: 586 bits (1511), Expect = 8.18e-211
Identity = 292/312 (93.59%), Postives = 301/312 (96.47%), Query Frame = 0


Query: 1   MSIKEVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSP 60
           MSIKEVPVIDL  QSLTEST+SSLLRACN+LGFFFIRNHGVSKEFYEKLISGADELFSSP
Sbjct: 1   MSIKEVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSP 60

Query: 61  EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSNLLD 120
           EETKQQLKMGVSSYTPRF+VSPFFESFRVSGPNFS SAA  GFTQSLFGHHATEFSNLLD
Sbjct: 61  EETKQQLKMGVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLD 120

Query: 121 EYGSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF 180
           EYG KMKEVSKRVVKLLLN+L DNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEE+EVF
Sbjct: 121 EYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEIEVF 180

Query: 181 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLRSAV 240
           KKHTDLSYVTIVF ND+RGLQMRSKGGDWID+R SED ILVVNIGDYMEAWSNGRLRSAV
Sbjct: 181 KKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNGRLRSAV 240

Query: 241 HRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFRENNYE 300
           HRVVLRKNI KRFSIAFLSTPEDESREI+APSEVVGEGKSRLYRSFS+QQYKHFRENNYE
Sbjct: 241 HRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENNYE 300

Query: 301 KVGATIKDFVGI 312
           KVGATIKDFVGI
Sbjct: 301 KVGATIKDFVGI 312

[Close]

BLAST of Csor.00g001870 vs. ExPASy TrEMBL
Match: A0A6J1GPF2 (gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111455932 PE=3 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 9.98e-196
Identity = 272/312 (87.18%), Postives = 289/312 (92.63%), Query Frame = 0


Query: 1   MSIKEVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSP 60
           MSI+EVPVID+SSQ+LTESTASSL RAC ELGFFFI+NHG+SKEFY+KLISG D+LFSSP
Sbjct: 1   MSIEEVPVIDISSQTLTESTASSLSRACTELGFFFIKNHGISKEFYQKLISGIDQLFSSP 60

Query: 61  EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSNLLD 120
           EETKQ+LK GVSSYTPRFVVSPF ESF++SGP FS SA  HGFTQS FGH ATEFSNLLD
Sbjct: 61  EETKQKLKQGVSSYTPRFVVSPFLESFKLSGPKFSASAVAHGFTQSQFGHQATEFSNLLD 120

Query: 121 EYGSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF 180
           EYGSKM EVSK+VVKLLL IL DNIENKFYDPEFSNC  YLRIN +TPL MN EEEVEVF
Sbjct: 121 EYGSKMTEVSKKVVKLLLKILGDNIENKFYDPEFSNCTSYLRINSYTPLGMNDEEEVEVF 180

Query: 181 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLRSAV 240
           KKHTDLSY TIVFPNDMRGLQMRSKGGDWI++R SED  LVVNIGDYMEAWSNGRLRSA+
Sbjct: 181 KKHTDLSYATIVFPNDMRGLQMRSKGGDWINVRPSEDGNLVVNIGDYMEAWSNGRLRSAL 240

Query: 241 HRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFRENNYE 300
           HRVVLRKNITKRFSIAFLSTPEDESREI+APSEVVGEGKSRLYRSFSIQQYKHFRENNY+
Sbjct: 241 HRVVLRKNITKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENNYK 300

Query: 301 KVGATIKDFVGI 312
           KVGATIKDFVGI
Sbjct: 301 KVGATIKDFVGI 312

[Close]

BLAST of Csor.00g001870 vs. ExPASy TrEMBL
Match: A0A6J1GMT1 (gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111455933 PE=3 SV=1)

HSP 1 Score: 524 bits (1349), Expect = 3.95e-186
Identity = 258/312 (82.69%), Postives = 278/312 (89.10%), Query Frame = 0


Query: 1   MSIKEVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSP 60
           MSI+EVPVID+SSQSLTESTASSL RAC ELGFFFI+NHG+SKEFY+K ISG D LFSSP
Sbjct: 1   MSIEEVPVIDISSQSLTESTASSLFRACTELGFFFIKNHGISKEFYQKFISGIDLLFSSP 60

Query: 61  EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSNLLD 120
           EETKQ+LK GVSSYTPRFV+SPF E+F+ SGPNFS S+   G TQS FGH ATEFSNLLD
Sbjct: 61  EETKQRLKHGVSSYTPRFVISPFLETFKFSGPNFSASSTAQGVTQSQFGHQATEFSNLLD 120

Query: 121 EYGSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF 180
           EYGSKM EVSKR++KLLLNIL DNIENKFYDPEFSNCNGYLRING+TPL MN +EEVEVF
Sbjct: 121 EYGSKMAEVSKRILKLLLNILGDNIENKFYDPEFSNCNGYLRINGYTPLGMNDDEEVEVF 180

Query: 181 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLRSAV 240
           KKHTD+SYVTIVFPND+RGLQMRSKGGDWID   SEDD LVVNIGD +EAWSNGRLRSA+
Sbjct: 181 KKHTDVSYVTIVFPNDIRGLQMRSKGGDWIDAMPSEDDTLVVNIGDLLEAWSNGRLRSAI 240

Query: 241 HRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFRENNYE 300
           HRVVLRKN  KRFSI + STPEDESREIHAPSEVVGEGKSRLYR FS QQYK FR  NYE
Sbjct: 241 HRVVLRKNNMKRFSIVYTSTPEDESREIHAPSEVVGEGKSRLYRPFSTQQYKDFRNKNYE 300

Query: 301 KVGATIKDFVGI 312
           K+G TIKDFVGI
Sbjct: 301 KIGETIKDFVGI 312

[Close]

BLAST of Csor.00g001870 vs. ExPASy TrEMBL
Match: A0A6J1JQS0 (gibberellin 20-oxidase-like protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488063 PE=3 SV=1)

HSP 1 Score: 504 bits (1299), Expect = 3.02e-178
Identity = 252/309 (81.55%), Postives = 272/309 (88.03%), Query Frame = 0


Query: 1   MSIKEVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSP 60
           MSI+EVPVIDL  +SLTES ASSLLRAC+ELGFFFIRNH VSKEFY+KLIS ADE FSSP
Sbjct: 1   MSIEEVPVIDLGCKSLTESIASSLLRACSELGFFFIRNHVVSKEFYKKLISSADEFFSSP 60

Query: 61  EETKQQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSNLLD 120
           EETKQ+L MGVSSYTPRFVVSPFFESFRVSGPNFS S    GFT SLFGH   EFSNLLD
Sbjct: 61  EETKQKLTMGVSSYTPRFVVSPFFESFRVSGPNFSASVVALGFTPSLFGHQTIEFSNLLD 120

Query: 121 EYGSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVF 180
           EYGSK+ E+SKRV+KLLL+IL DNIENK YDPEFSN NGYLRIN +TPL MN +EEVEVF
Sbjct: 121 EYGSKITEISKRVLKLLLSILGDNIENKLYDPEFSNSNGYLRINSYTPLGMNDDEEVEVF 180

Query: 181 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLRSAV 240
           KKHTD+SYVTIVFPND+RGLQMRSKGG+WID++ SED ILVVNIGD +EAWSN RLRSA+
Sbjct: 181 KKHTDISYVTIVFPNDIRGLQMRSKGGEWIDVKPSEDGILVVNIGDLLEAWSNERLRSAI 240

Query: 241 HRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFRENNYE 300
           HRVVLRKN  KRFSI + STPEDESREIHAPSEVVGEGKS LYRSFS QQYKHFRENNYE
Sbjct: 241 HRVVLRKNNMKRFSIVYTSTPEDESREIHAPSEVVGEGKSSLYRSFSTQQYKHFRENNYE 300

Query: 301 KVGATIKDF 309
           KVG  +  F
Sbjct: 301 KVGKLLNTF 309

[Close]

BLAST of Csor.00g001870 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 283.9 bits (725), Expect = 1.6e-76
Identity = 159/316 (50.32%), Postives = 213/316 (67.41%), Query Frame = 0


Query: 5   EVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSPEETK 64
           ++PV+DL +Q +  S  SSL  AC E GFF++ NHG+SKE + K+ S + ++F +P E+K
Sbjct: 9   QLPVLDL-TQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVFKAPLESK 68

Query: 65  QQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLF-GHHATEFSNLLDEYG 124
             LK+G  SYTPR++ SP+FES  VSGP+FS SA        LF  HH  E    + EYG
Sbjct: 69  --LKLGPISYTPRYIASPYFESLVVSGPDFSDSAKAS--ADVLFQDHHKPELRETMQEYG 128

Query: 125 SKMKEVSKRVVKLLLNI-LRDNIENKFYDPEFSNCNGYLRINGFT-PLDMNAEEE-VEVF 184
           +KM E+SKR++K+LL + L D    + Y  +FSNC+GYLR+  +T P D+  +EE VE  
Sbjct: 129 AKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEELVEGL 188

Query: 185 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLRSAV 244
             HTD+S +TIV+ + + GLQMRSK G WIDI    +D LVVNIGD M+AWSNGRLRS+ 
Sbjct: 189 GMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPC-NDFLVVNIGDLMQAWSNGRLRSSE 248

Query: 245 HRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFRENN-- 304
           HRVVLRK +  R S+AF    EDE + I AP E+VGEGK R Y+SF   +Y  FR++N  
Sbjct: 249 HRVVLRK-LVNRVSLAFFLCFEDE-KVILAPQEIVGEGKQRSYKSFKCSEYLKFRQSNEE 308

Query: 305 --YEKVGATIKDFVGI 313
             +EK+G T+KDF G+
Sbjct: 309 GKFEKIGFTVKDFAGL 316

[Close]

BLAST of Csor.00g001870 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 3.9e-67
Identity = 145/326 (44.48%), Postives = 213/326 (65.34%), Query Frame = 0


Query: 5   EVPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSPEETK 64
           E+PV D+ S+ L+ES+ +SL  AC E GFF++ NHGVS++ Y+KL   +  +F   +E K
Sbjct: 4   ELPVFDI-SKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEEK 63

Query: 65  QQLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHAT--EFSNLLDEY 124
             +KMG S+YTPRF+ SPFFES RVSGP+F  SA +   +   F   AT  EFS L+ EY
Sbjct: 64  --MKMGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFSGLMKEY 123

Query: 125 GSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFT-PLDM-----NAEEE 184
           G KM ++ ++++K +L+   D++ +K+Y+ EF NC+GY RIN +T P D      N +E+
Sbjct: 124 GEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQ 183

Query: 185 --VEVFKKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNG 244
             +E    HTD+S +TIV  +D+ GLQ+R++ G  +   + +D+ LVVN+GD + AW+NG
Sbjct: 184 DLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLLHAWTNG 243

Query: 245 RLRSAVHRVVLRKN--ITKRFSIAFLSTPEDESREIHAPSEVVG--EGKSRLYRSFSIQQ 304
           RLRS+ HRV+L++   +  RFS+AF     D+ + + AP EVVG  EG  R++RSF    
Sbjct: 244 RLRSSQHRVILKRRGFVGNRFSLAFFWC-FDDGKVVFAPDEVVGGCEG-MRVFRSFKCGD 303

Query: 305 YKHFRENN----YEKVGATIKDFVGI 313
           Y  FRE+N    +EKVG T++DF  I
Sbjct: 304 YLRFRESNEKGKFEKVGDTVEDFARI 321

[Close]

BLAST of Csor.00g001870 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 194.9 bits (494), Expect = 9.7e-50
Identity = 106/201 (52.74%), Postives = 140/201 (69.65%), Query Frame = 0


Query: 119 LDEYGSKMKEVSKRVVKLLLNI-LRDNIENKFYDPEFSNCNGYLRINGFT-PLDMNAEEE 178
           + EYG+KM E+SKR++K+LL + L D    + Y  +FSNC+GYLR+  +T P D+  +EE
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 60

Query: 179 -VEVFKKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGR 238
            VE    HTD+S +TIV+ + + GLQMRSK G WIDI    +D LVVNIGD M+AWSNGR
Sbjct: 61  LVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPC-NDFLVVNIGDLMQAWSNGR 120

Query: 239 LRSAVHRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFR 298
           LRS+ HRVVLRK +  R S+AF    EDE + I AP E+VGEGK R Y+SF   +Y  FR
Sbjct: 121 LRSSEHRVVLRK-LVNRVSLAFFLCFEDE-KVILAPQEIVGEGKQRSYKSFKCSEYLKFR 180

Query: 299 ENN----YEKVGATIKDFVGI 313
           ++N    +EK+G T+KDF G+
Sbjct: 181 QSNEEGKFEKIGFTVKDFAGL 198

[Close]

BLAST of Csor.00g001870 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 108.6 bits (270), Expect = 9.1e-24
Identity = 87/293 (29.69%), Postives = 145/293 (49.49%), Query Frame = 0


Query: 5   EVPVID---LSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSPE 64
           E+PVID   L S S  +S    L  AC + GFF + NHG+   F EKL +   E F+ P 
Sbjct: 52  EIPVIDMTRLCSVSAMDSELKKLDFACQDWGFFQLVNHGIDSSFLEKLETEVQEFFNLPM 111

Query: 65  ETKQQLKMGVSSYTPRFVVSPFFESFRVS-GPNF-STSAATHGFTQSLFGHHATEFSNLL 124
           + KQ+L      +     V+   E+ ++  G  F  T+         LF      F   L
Sbjct: 112 KEKQKLWQRSGEFEGFGQVNIVSENQKLDWGDMFILTTEPIRSRKSHLFSKLPPPFRETL 171

Query: 125 DEYGSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEV 184
           + Y S++K ++K +   + ++L   I+++  +  F +    ++IN + P      ++V  
Sbjct: 172 ETYSSEVKSIAKILFAKMASVL--EIKHEEMEDLFDDVWQSIKINYYPPCPQ--PDQVMG 231

Query: 185 FKKHTDLSYVTIVFP-NDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLRS 244
             +H+D + +TI+   N + GLQ++ K G W+ ++    D LVVN+G+ +E  +NGR RS
Sbjct: 232 LTQHSDAAGLTILLQVNQVEGLQIK-KDGKWVVVKPLR-DALVVNVGEILEIITNGRYRS 291

Query: 245 AVHRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQY 292
             HR V+     +R S+A   +P  E+  I     +V   K  L++S S Q+Y
Sbjct: 292 IEHRAVVNSE-KERLSVAMFHSPGKET-IIRPAKSLVDRQKQCLFKSMSTQEY 336

[Close]

BLAST of Csor.00g001870 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 107.8 bits (268), Expect = 1.6e-23
Identity = 79/316 (25.00%), Postives = 150/316 (47.47%), Query Frame = 0


Query: 6   VPVIDLSSQSLT------ESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSS 65
           +PVID S+++L       + T + + +A  + G+F      +  E  + +    +ELF  
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFDL 71

Query: 66  PEETKQQ--LKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSN 125
           P +TK +   K     Y  ++ + P +ES  +   + +       FT+ L+      FS 
Sbjct: 72  PLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEK--VDAFTEKLWPQGNISFST 131

Query: 126 LLDEYGSKMKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEV 185
            +  +  K+ E+   + ++++        +K+ D    + N  LR+  +   D   EE  
Sbjct: 132 TIQSFSKKLSELDITIRRMIMESFG---LDKYIDEHLHSTNYLLRVMKYKGPD--TEETK 191

Query: 186 EVFKKHTDLSYVTIVFPNDMRGLQMRSKGGDWIDIRSSEDDILVVNIGDYMEAWSNGRLR 245
                HTD + VTI++ N + GL++++K  +WI ++ ++D   V+ IGD + A  NGRL 
Sbjct: 192 VGLNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQDSFTVM-IGDSLYALLNGRLH 251

Query: 246 SAVHRVVLRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFREN 305
           S  HRV++    T R+S+   S P+     + +P E+V E   RL++ F   ++  F   
Sbjct: 252 SPYHRVMMTGTET-RYSLGLFSIPK-AGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYT 311

Query: 306 NY-EKVGATIKDFVGI 313
              ++  + +K + GI
Sbjct: 312 EAGQRSQSALKTYCGI 317

The following BLAST results are available for this feature:

BLAST of Csor.00g001870 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBY0	5.5e-66	44.48	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]
Q39111	6.4e-22	29.91	Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1	[more]
Q9SQ80	1.9e-21	27.78	Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1	[more]
O24648	3.2e-21	27.48	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1	[more]
Q9XFR9	1.6e-20	26.85	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]

BLAST of Csor.00g001870 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6576056.1	3.23e-224	100.00	Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_022953358.1	3.18e-215	96.15	gibberellin 20-oxidase-like protein [Cucurbita moschata]	[more]
XP_022991455.1	1.69e-210	93.59	gibberellin 20-oxidase-like protein [Cucurbita maxima]	[more]
KAG6576054.1	1.85e-198	88.46	Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_022953360.1	2.06e-195	87.18	gibberellin 20-oxidase-like protein [Cucurbita moschata]	[more]

BLAST of Csor.00g001870 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GN55	1.54e-215	96.15	gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC11145593...	[more]
A0A6J1JSZ7	8.18e-211	93.59	gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111488065 ...	[more]
A0A6J1GPF2	9.98e-196	87.18	gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC11145593...	[more]
A0A6J1GMT1	3.95e-186	82.69	gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC11145593...	[more]
A0A6J1JQS0	3.02e-178	81.55	gibberellin 20-oxidase-like protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of Csor.00g001870 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23340.1	1.6e-76	50.32	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51310.1	3.9e-67	44.48	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G23340.2	9.7e-50	52.74	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G78550.1	9.1e-24	29.69	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	1.6e-23	25.00	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..301 e-value: 3.4E-59 score: 202.8
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 180..260 e-value: 5.4E-14 score: 52.5
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 6..69 e-value: 1.7E-14 score: 54.6
None	No IPR available	PANTHER	PTHR47990:SF9	GIBBERELLIN 20-OXIDASE-LIKE PROTEIN	coord: 4..304
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 4..304
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 2..298
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 165..261 score: 8.462046

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g001870.m01	Csor.00g001870.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity