CsaV3_7G028580 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_7G028580 vs. NCBI nr
Match: XP_004136188.1 (isoamylase 2, chloroplastic [Cucumis sativus] >KGN44865.1 hypothetical protein Csa_016566 [Cucumis sativus])

HSP 1 Score: 1795.0 bits (4648), Expect = 0.0e+00
Identity = 885/885 (100.00%), Postives = 885/885 (100.00%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
           VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
           TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
           YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
           NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
           VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 886
           RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885

BLAST of CsaV3_7G028580 vs. NCBI nr
Match: XP_008451396.1 (PREDICTED: isoamylase 2, chloroplastic [Cucumis melo])

HSP 1 Score: 1687.5 bits (4369), Expect = 0.0e+00
Identity = 830/884 (93.89%), Postives = 852/884 (96.38%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MATFLSSLVIQPFCV+NCGVTES KLA SDHLTYGQKTKYQ GKMDEARMLAHGE+KVGA
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
           VKSSHRNLSK Y  SGI VGKSGQRLGIGGKSKEQR+VATYLFRTEFGDLVNVFVGK GS
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
            FTVNIE+ SMQLVS DE LLLSW VYRSDSA VT NF+SSPPD TTGATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLK+PMGVDSGSSEIRSH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SGDGS+NFSIFSS+AES+VLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
           YGYQCKG KSHENQDGLEVSRIVVDPYAKILAPSIPKS  QGLG P KFLGQIS VP FD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLFVYRLNV+RFT+DKSSQLPADIAGTFSGLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVI+EVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           TS +GALQGIDDSSYYF NRVANLEE+SALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
           NASFLL+GHHGELLSRPPFVEAIAFDP+LSKTKLVADFWDPQEL SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPT+ LKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
           VAFND+RSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQ+RNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWEDASCKFLAVMLRADKEENESITENPKTRS+IFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 885
           RVVDTALPFPGFFSSDGELVPMTGSVTYE+QAHSCALFEAKSAN
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of CsaV3_7G028580 vs. NCBI nr
Match: KAA0057598.1 (isoamylase 2 [Cucumis melo var. makuwa] >TYK20996.1 isoamylase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1686.0 bits (4365), Expect = 0.0e+00
Identity = 829/884 (93.78%), Postives = 851/884 (96.27%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MATFLSSLVIQPFCV+NCGVTES KLA SDHLTYGQKTKYQ GKMDEARMLAHGE+KVGA
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
           VKSSHRNLSK Y  SGISVGKSGQRLGIGGKSKEQR+VATYLFRTEFGDLVNVFVGK GS
Sbjct: 61  VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
            FTVNIE+ SMQLVS DE LLLSW VYRSDSA VT NF+SSPPD TTGATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLK+PMGVDSGSSEIRSH+KTSFSVPVGFGRGYPSP GLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240

Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SGDGS+NFSIFSS+AES+VLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEG S FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300

Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
           YGYQCKG KSHENQDGLEVSRIVVDPYAKILAPSIPKS  QGLG P KFLGQIS VP FD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLFVYRLNV+RFT+DKSSQLPADIAGTFSGLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVI+EVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           TS +GALQGIDDSSYYF NRVANLEE+SALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
           NASFLL+GHHGELLSRPPFVEAIAFDP+LSKTKLVADFWDPQEL SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPT+ LKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
           VAFND+RSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQ+RNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWEDASCKFLAVMLRADKEENESITENPKTRS+IFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 885
           RVVDTALPFPGFFSSDGELVPMTGSVTYE+QAHSCALFEAKSAN
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of CsaV3_7G028580 vs. NCBI nr
Match: XP_038898155.1 (isoamylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 1581.6 bits (4094), Expect = 0.0e+00
Identity = 774/885 (87.46%), Postives = 822/885 (92.88%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MATFLSSLVIQPFCVYN GVTES KLA  DH TYGQK KY  GKMDEARMLAHGE++VG+
Sbjct: 1   MATFLSSLVIQPFCVYNSGVTESFKLAVGDHHTYGQKIKYHSGKMDEARMLAHGESRVGS 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
            KSSHRNLSK YA SGI VGKSGQR G GGKSKE  +VATYLFRTEFGDLVNVFVGK GS
Sbjct: 61  TKSSHRNLSKVYATSGILVGKSGQRFGTGGKSKEHVKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
            F VN+E+ S+QL SI++ LLLSWGVYRSDSA+VTPNF+SS PDET GATETPFVKTS G
Sbjct: 121 GFMVNVEISSVQLASIEKTLLLSWGVYRSDSAIVTPNFQSSLPDETIGATETPFVKTSGG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
           KF+VELEFDAKHTPFYLSF+LKYP+GVDSGSSEIRSH+KTSF+VPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHTPFYLSFLLKYPVGVDSGSSEIRSHRKTSFTVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SGDGS+NFSIFSS+AES+VLCLYNDSTSEKP+LELDLD YINRSG+IWHASFE AS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDSYINRSGSIWHASFESASNFVS 300

Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
           YGY+CKGSK HENQDG+EV+RIVVDPYAKIL  S+ K+ GQGLGLP KFLGQI KVP FD
Sbjct: 301 YGYRCKGSKFHENQDGVEVNRIVVDPYAKILTASVAKTLGQGLGLP-KFLGQICKVPAFD 360

Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLF YRLNV+RFT+DKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVCPNLPMEKLFAYRLNVKRFTVDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
           EPIFQFDEKEG YFPFHFFSP NNYGPSG SISAINSMK MVK+LHANGVEVILEVV+TH
Sbjct: 421 EPIFQFDEKEGLYFPFHFFSPANNYGPSGVSISAINSMKGMVKQLHANGVEVILEVVFTH 480

Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           T+ +GALQGIDDSSYYF NRVANLE KSALNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TAMDGALQGIDDSSYYFVNRVANLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540

Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
           NAS LLRGHHGE LSR PFVEAIAFDPLLSKTKLVADFWDPQEL S+ET FPHWKRWAEV
Sbjct: 541 NASSLLRGHHGEFLSRSPFVEAIAFDPLLSKTKLVADFWDPQELVSEETSFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKFC+D+RDFFRGEGLISSLATRLCGSGDVFS+GRGPAFSFNFI RNVGLPLVDLVSFS
Sbjct: 601 NSKFCNDVRDFFRGEGLISSLATRLCGSGDVFSNGRGPAFSFNFITRNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NS LASELSWNCGEEGPTSN++VLEKRLKQIRNFIFV+FVSLGVPVLNMGDECGQS+GGS
Sbjct: 661 NSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVVFVSLGVPVLNMGDECGQSAGGS 720

Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
           VAFND+RSFNWDLLKTDFGTQTTQFI FLSSFRSRRFDLFQ+RNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWED SCKFLAVMLRADKEEN SITEN K RS+IFM FNASDQSE+VALPEP EGTSWF
Sbjct: 781 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSETVALPEPSEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 886
           R+VDTAL FPGFFSSDGE VP TGSVTYE+QAHSCALFEAKS ND
Sbjct: 841 RLVDTALSFPGFFSSDGEQVPTTGSVTYEMQAHSCALFEAKSFND 884

BLAST of CsaV3_7G028580 vs. NCBI nr
Match: KAG7014422.1 (Isoamylase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1517.7 bits (3928), Expect = 0.0e+00
Identity = 736/885 (83.16%), Postives = 808/885 (91.30%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MATFLSSL IQPFC YN G TES KLA +D+ TYGQK  YQ GK+DEARM AHGE+KVG 
Sbjct: 24  MATFLSSL-IQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 83

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
            KS HRN+ K YA SGISVGKSGQ+ G GGK++E R+VATYLFRTEFGDLV  FVGK+GS
Sbjct: 84  TKSLHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 143

Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
            F V+IE+ S++L SIDE LLLSWGVYRS+SA+VTPNF+S  P+ET GATETPFV+TSEG
Sbjct: 144 GFLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 203

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
           KF+VELEFDAKH PFYLSF+LKYP+GVDSGSSEI+SH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 204 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 263

Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SGDGS+NFSIFSS+AES+VLCLYNDSTSEKP+LELDLDPYINRSGNIWH SFEGAS FVS
Sbjct: 264 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNFVS 323

Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
           YGY+C+GSK HENQD  EVSRI+VDPYAKIL  S PK+ GQG+GLPSKFLGQI  VP F 
Sbjct: 324 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICNVPAFS 383

Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLF+YRLNV+ FT+DKSSQLP DIAGTFSGLT+KLLHFK LGVNAVLL
Sbjct: 384 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLL 443

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
           EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANGVEVILEVV+TH
Sbjct: 444 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 503

Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           T+ +GALQGIDDS YYF NRVAN E KSALNCN+PIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 504 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFI 563

Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
           NAS LL+GHHGELLSRPPFVEAIAFDPLLSKTK++A+FWDPQEL  KETRFPHWKRWAE+
Sbjct: 564 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAEL 623

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKF +D+++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 624 NSKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 683

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +S LASELSWNCGEEGPTSN++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 684 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 743

Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
           VAF D++SFNWD LKTDFGTQTT+FIAF SSFRSRRFDLFQ+R+FLKGENIDWFDNNQSP
Sbjct: 744 VAFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFDNNQSP 803

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
           P+WED SCKFLAVMLRADKEE+ SITENPK +S+IFM FNASDQS SVALPEP EGT+WF
Sbjct: 804 PEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 863

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 886
           R+VDTAL FPGFFSSDGE VP T  VTYE+QAHSC LFEAKS ND
Sbjct: 864 RLVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFND 907

BLAST of CsaV3_7G028580 vs. ExPASy Swiss-Prot
Match: Q8L735 (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2)

HSP 1 Score: 942.6 bits (2435), Expect = 3.3e-273
Identity = 477/881 (54.14%), Postives = 628/881 (71.28%), Query Frame = 0

Query: 14  CVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGAVKSSHRNLSKTYA 73
           C  N G+T + K   S  L  G+K K + G   E  M          V S+     ++Y 
Sbjct: 13  CCLNNGITRTWKF-PSARLFTGRKNKIKLG--SETLMFTRKRFMGDLVTSA----LQSYQ 72

Query: 74  KSGISVGKSGQRLGIGGKSK-----EQRRVATYLFRTEFGDLVNVFVGKKGSTFTVNIEV 133
            S I   K+   L     S+     + ++V +Y FRT+ G LV V V KK   +++ + V
Sbjct: 73  FSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYV 132

Query: 134 PSMQLVSIDEA-LLLSWGVYRSDSALVTP-NFESSPPDETTGATETPFVKTSEGKFSVEL 193
            S++L   D++ L++ WGVYRSDS+   P +FE+S  D  T  TET FVK+S  +  + L
Sbjct: 133 SSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGL 192

Query: 194 EFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSIS-GDGS 253
           EFD K +PFYLSF LK   G D    E+ +H+ T F +PVGF  G+P PLGLS    D S
Sbjct: 193 EFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDS 252

Query: 254 VNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGYQC 313
            NFS FS S+ ++VLCLY+DST++KP LELDLDPY+NR+G++WHAS +    FV YGY+C
Sbjct: 253 WNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRC 312

Query: 314 KGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFDWDGEV 373
           K +   +    +E   IV+DPYA ++  S+ +          K+LG +SK P+FDW  +V
Sbjct: 313 KETAHSKEDVDVEGEPIVLDPYATVVGKSVSQ----------KYLGSLSKSPSFDWGEDV 372

Query: 374 HPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQ 433
            PN+P+EKL VYRLNV+ FT  +SS+LP+++AGTFSG+ +K+ H K LG NAVLLEPIF 
Sbjct: 373 SPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFS 432

Query: 434 FDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTSGNG 493
           F E++GPYFPFHFFSP + YGPS +  SA+NSMK MVK+LH+ G+EV+LEVV+THT+ +G
Sbjct: 433 FSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG 492

Query: 494 ALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFL 553
           AL+GIDDSSYY+  R  +L+ KS LNCNYP+VQQL+L+SLRYWVTEFHVDGFCF+NAS L
Sbjct: 493 ALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSL 552

Query: 554 LRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFC 613
           LRG HGE LSRPP VEAIAFDPLL++TKL+AD WDP E+  KE RFPHWKRWAE+N+++C
Sbjct: 553 LRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYC 612

Query: 614 SDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSNLA 673
            ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN GL LVD+VSFS   LA
Sbjct: 613 RNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELA 672

Query: 674 SELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFND 733
           SELSWNCGEEG T+   VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS     
Sbjct: 673 SELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLES 732

Query: 734 KRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQWED 793
           ++ F+W+LL + FGTQ TQFI+F++S R+RR D+FQ R+FLK ENI W+ N+Q+ P+WED
Sbjct: 733 RKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWED 792

Query: 794 ASCKFLAVMLRADKEENE--SITENPKTRSN-IFMVFNASDQSESVALPEPLEGTSWFRV 853
            + KFLA+ ++++ EE E  S+ E  + +SN +F+ FNASD  ESV LP   +G+ W R+
Sbjct: 793 PASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRL 852

Query: 854 VDTALPFPGFFSSDGELV----PMTGSVTYEIQAHSCALFE 880
           VDTALPFPGFFS +GE V    P+   V YE++ +SC LFE
Sbjct: 853 VDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of CsaV3_7G028580 vs. ExPASy Swiss-Prot
Match: Q6AU80 (Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=1 SV=1)

HSP 1 Score: 764.2 bits (1972), Expect = 1.6e-219
Identity = 372/716 (51.96%), Postives = 506/716 (70.67%), Query Frame = 0

Query: 183 SVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSISG 242
           + EL FD    PFYLSF+L      D+  +EIR+H+KTSF VPVG G G P+PLG+SISG
Sbjct: 105 AAELSFDPTRAPFYLSFLL-----TDASGAEIRTHRKTSFRVPVGVGPGSPAPLGMSISG 164

Query: 243 DGSVNFSIFSSSAESLVLCLY----NDSTSEKPLLELDLDPYINRSGNIWHASFEGASKF 302
           DG+VNF+++S +A ++ L LY         ++P LE+DLDPYI+R+GN+WH S      +
Sbjct: 165 DGAVNFAVYSKNANAVSLYLYAAAVGGGGGDEPALEIDLDPYIHRTGNVWHVSLASVDGY 224

Query: 303 VSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSS--GQGLGLPS-KFLGQISK 362
           VSY + C G           + R ++DPYAK++   +  +S   +G+  PS +    ++ 
Sbjct: 225 VSYAFCCGG-----------IRRPLLDPYAKVIGDFVSSNSVYDEGVTAPSMRCFASLAI 284

Query: 363 VPTFDWDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGV 422
            P+++W  + HP LP+EKL VYR NV  FT D+SS LP D AGTF+GL+ K+ HF++LGV
Sbjct: 285 APSYNWGRDRHPRLPLEKLVVYRANVALFTKDRSSGLPDDAAGTFTGLSAKVEHFRSLGV 344

Query: 423 NAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILE 482
           NA+LLEP+F F + +GPYFP+HFFSP N Y   G S+SAI SMK+MV+ +H NG+EV+LE
Sbjct: 345 NAILLEPVFPFHQVKGPYFPYHFFSPMNLYSSKGLSVSAIKSMKDMVRVMHRNGIEVLLE 404

Query: 483 VVYTHTSGNG------ALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWV 542
           VV+THT+         +++GID+SSYY  N +A   + S LNCN+P+ Q+L+LDSLR+WV
Sbjct: 405 VVFTHTAEGESECQTISMRGIDNSSYYIANGIAGC-KASILNCNHPVTQKLILDSLRHWV 464

Query: 543 TEFHVDGFCFVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKET 602
            +FHVDGFCF+NA FL+RG  GE LSRPP +EAI FDP+LS TK++AD W P ++ + + 
Sbjct: 465 LDFHVDGFCFINAPFLVRGPGGEYLSRPPLLEAITFDPVLSMTKIIADPWSPLDISNVQF 524

Query: 603 RFPHWKRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNV 662
            FPHWKRWAEVN++F  D+R F + E LIS LATRLCGSGD+FS  RGPAFSFN ++RN 
Sbjct: 525 PFPHWKRWAEVNTRFSIDVRKFLKREALISDLATRLCGSGDLFST-RGPAFSFNHVSRNS 584

Query: 663 GLPLVDLVSFSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNM 722
           GL LVDLVSFSN +L SE SWNCGEEGP+ N  VL+ RL+QIRNF+F+LFVSLGVPVLNM
Sbjct: 585 GLSLVDLVSFSNDDLLSESSWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLGVPVLNM 644

Query: 723 GDECGQSSGGSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGE 782
           GDECG S+ GSV++ D+   NW  +KT F  + T FI+FL++ RSRR D+FQ R FLK E
Sbjct: 645 GDECGHSAAGSVSYKDRGPLNWRGMKTTFVKEVTGFISFLTALRSRRGDIFQRREFLKLE 704

Query: 783 NIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVA 842
           NI W+ ++   P W+D +  FL + + A+ +E  +       R ++++ FNA+++S S A
Sbjct: 705 NIHWYGSDLCEPGWDDPTSNFLCMHINAEVDEMAA----DSVRGDLYICFNANEESVSAA 764

Query: 843 LPEPLEGTSWFRVVDTALPFPGFFS--SDGELVPMTGSVTYEIQAHSCALFEAKSA 884
           LP   EG+ W R+VDT+L FPGFF+  S+ ++  + G  +Y ++AH+C LFE+KSA
Sbjct: 765 LPALAEGSVWLRLVDTSLAFPGFFATESNPKVQQVPGLSSYHVEAHTCVLFESKSA 798

BLAST of CsaV3_7G028580 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 374.0 bits (959), Expect = 4.6e-102
Identity = 246/701 (35.09%), Postives = 360/701 (51.36%), Query Frame = 0

Query: 231 GYPSPLGLSISGDGSVNFSIFSSSAESLVLCLY-----NDSTSEKPLLELDLDPYINRSG 290
           G  SPLG+S   D  +NF++FS +A S+ LCL       D T +  ++EL LDP +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 291 NIWHASFEGAS-KFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLG 350
           + WH   E      V YGY+  G    +     + S +++DPYAK++      S G    
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGH--SSFGDSSQ 207

Query: 351 LPSKFLGQIS-KVPTFDW-DGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSG 410
             ++F G    +   FDW D    PN+P + L +Y +NV  FT D+SS +   I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267

Query: 411 LTKKLLHFKNLGVNAVLLEPIFQFDEKE--------------GPYFPFHFFSPTNNYGP- 470
             +K+ H ++LG+NAV L P+F+FDE E                Y   +FF+P + Y   
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327

Query: 471 SGASISAINSMKEMVKELHANGVEVILEVVYTHTSG-------NGALQGIDDSSYYF--- 530
            G  I A    KEMVK LH+ G+EVIL+VVY HT+          + +GID+  YY    
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387

Query: 531 TNRVANLEE-KSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRGHHGELLSR 590
            N++ N     + LNCN+P+V +L+LDSLR+WVTE+HVDGF F  AS L R   G  LS 
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447

Query: 591 PPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFCSDIRDFFRGE- 650
           PP + AIA D +LS+ K++A+ WD   L     +FP+W RWAE N  +  D+R F +G+ 
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGL-YLVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507

Query: 651 GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFS-----------NSN 710
           G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++           N  
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567

Query: 711 LASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSS-GGSVA 770
                SWNCG EG T +  +   R +Q++NF   L +S G P++ MGDE G +  G + +
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627

Query: 771 FNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRR-FDLFQNRNFLKGENIDWFDNNQSPP 830
           +    S N    K     +   F  F    + R    + ++ NFL    I W ++N    
Sbjct: 628 YGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDN---- 687

Query: 831 QWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWFR 882
            W+++  KFLA  L      ++ I        +I++ FNA D      +P+P  G  WFR
Sbjct: 688 -WDNSESKFLAFTL------HDGIGGR-----DIYVAFNAHDYFVKALIPQPPPGKQWFR 747

BLAST of CsaV3_7G028580 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.5e-100
Identity = 247/723 (34.16%), Postives = 368/723 (50.90%), Query Frame = 0

Query: 212 SEIRSHKKTSFSVPVGFGRGYPSPLGLSISGDGSVNFSIFSSSAESLVLCLY-----NDS 271
           +E R    +   +P  +  G   PLG+S   +G +NF++FS  A S++LCL       + 
Sbjct: 84  TEERGCTMSDTEMPFKYSSGKAFPLGVS-QVEGGLNFALFSQHASSVILCLKLPGRGTED 143

Query: 272 TSEKPLLELDLDPYINRSGNIWHASFEG-ASKFVSYGYQCKGSKSHENQDGLEVSRIVVD 331
                ++E  LD   N++G+IWH   EG  +  V YGY+  G +  +     + S +++D
Sbjct: 144 EKGADVVEFVLDQQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLD 203

Query: 332 PYAKILAPSIPKSSGQGLGLPSKFLGQIS-KVPTFDWDGEVH-PNLPMEKLFVYRLNVER 391
           PYAK++  S  K  G      S+  G        FDW  +   PNLP   L +Y +NV  
Sbjct: 204 PYAKLV--SGRKYFGVAEEKSSQHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEMNVRA 263

Query: 392 FTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQFDEKEGPYFP-------- 451
           FT D+SS L +   G++ GL  K+ H   LGVNAV L P+F++DE E   +P        
Sbjct: 264 FTADESSGLDSTSRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRDHMVN 323

Query: 452 ------FHFFSPTNNYGPSGAS-ISAINSMKEMVKELHANGVEVILEVVYTHTSGNG--- 511
                  +FF+P + Y  +G   ++A   +K+MVKELH  G+EVIL+VVY HT+      
Sbjct: 324 TWGYSTINFFAPMSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEADDAH 383

Query: 512 ----ALQGIDDSSYYFTNRVANLEE------KSALNCNYPIVQQLLLDSLRYWVTEFHVD 571
               + +GID+  YY  +   N E        + LNCN+P+V++L+LDSLR+WV E+H+D
Sbjct: 384 PYMTSFRGIDNKVYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEEYHID 443

Query: 572 GFCFVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWK 631
           GF F  AS L RG  G  L  PP ++ IA D +LS+ K++A+ WD   L     RFP+W 
Sbjct: 444 GFRFDLASVLCRGPDGCPLDAPPLIKEIAKDAVLSRCKIIAEPWDCGGL-YLVGRFPNWD 503

Query: 632 RWAEVNSKFCSDIRDFFRGE-GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPL 691
           RWAE N K+  D+R F +G+ G+    ATR+ GS D++  + R P    NF+  + G  L
Sbjct: 504 RWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHDGFTL 563

Query: 692 VDLVSFSNSN-----------LASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSL 751
            DLVS++  +                SWNCG EG T++L VL  R +Q++NF   L +S 
Sbjct: 564 CDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVALMISQ 623

Query: 752 GVPVLNMGDECGQSS-GGSVAFNDK---RSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFD 811
           G P++ MGDE G +  G + ++       +F W+ L+        +F + +  FR     
Sbjct: 624 GTPMMLMGDEYGHTRYGNNNSYGHDTCINNFQWEQLEQRRDGH-FRFFSEMIKFRHSNPI 683

Query: 812 LFQNRNFLKGENIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMV 871
           L ++R FL   ++ W ++      WE+   KFLA  +            +  +  +I++ 
Sbjct: 684 LRRDR-FLNKNDVTWHED-----CWENQESKFLAFTV-----------HDHNSGGDIYLA 743

Query: 872 FNASDQSESVALPEPLEGTSWFRVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALF 882
           FNA D      +P P     W RVVDT L  P     +G  VP TG   Y I  +S  L 
Sbjct: 744 FNAHDYFVDAVIPPPPHHKCWNRVVDTNLESPNDIVPEG--VPFTGP-KYRIAPYSSILL 781

BLAST of CsaV3_7G028580 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.4e-95
Identity = 234/689 (33.96%), Postives = 343/689 (49.78%), Query Frame = 0

Query: 231 GYPSPLGLSISGDGSVNFSIFSSSAESLVLCLY--NDSTSEKPLLELDLDPYINRSGNIW 290
           G P+PLG + + DG VNF+++S+ A +  LCL+  +D  +++   E+ LDP  NR+GN+W
Sbjct: 99  GMPAPLG-ATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVW 158

Query: 291 HASFEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAK-ILAPSIPKSSGQGLGLPS 350
           H   EG    + YGY+  G  +       +VS +VVDPYAK +++       G G     
Sbjct: 159 HVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDCWP 218

Query: 351 KFLGQIS-KVPTFDWDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKK 410
           +  G I     TFDW G++    P + L +Y +++  FT   SS +  +  GT+ G   K
Sbjct: 219 QMAGMIPLPYSTFDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSNV--EHPGTYIGAISK 278

Query: 411 LLHFKNLGVNAVLLEPIFQFDEKE----------GPYFPFHFFSPTNNYGPSG---ASIS 470
           L + K LGVN V L P  +F+E E            Y   +FFSP   Y   G       
Sbjct: 279 LDYLKELGVNCVELMPCHEFNELEYFSCSSKMNFWGYSTINFFSPMIRYSSGGIRNCGRD 338

Query: 471 AINSMKEMVKELHANGVEVILEVVYTHTS-GNG-----ALQGIDDSSYYFTNRVANLEEK 530
           AIN  K  V+E H  G+EVI++VV+ HT+ GN      + +GID+S+YY           
Sbjct: 339 AINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKGEFYNY 398

Query: 531 S----ALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRG--------------- 590
           S      NCN+P+V++ ++D LRYWVTE HVDGF F  AS + RG               
Sbjct: 399 SGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSPVE 458

Query: 591 ----HHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKF 650
                 G  L+ PP ++ I+ DP+L   KL+A+ WD   L  +  +FPHWK W+E N K+
Sbjct: 459 GDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWDAGGL-YQVGQFPHWKIWSEWNGKY 518

Query: 651 CSDIRDFFRG-EGLISSLATRLCGSGDVF-SDGRGPAFSFNFIARNVGLPLVDLVSFSNS 710
              +R F +G +G     A  LCGS  ++ + GR P  S NF+  + G  L DLV+++  
Sbjct: 519 RDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNKK 578

Query: 711 NLAS-----------ELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGD 770
             +S            LSWNCGEEG  + L V   R +Q+RNF   L VS GVP+  MGD
Sbjct: 579 YNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMGD 638

Query: 771 ECGQSSGG--SVAFNDK--RSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLK 830
           E G + GG  +   +D     F WD  K +  +   +F + ++ FR ++ +     +F  
Sbjct: 639 EYGHTKGGNNNTYCHDHYVNYFRWD--KKEESSDLQRFCSLMTKFR-KQCESLGLADFPT 698

Query: 831 GENIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSES 857
            + + W  +    P W + S +F+A             +   +T+  I++ FNAS     
Sbjct: 699 AQRLHWHGHQPGKPDWSETS-RFVA------------FSTKDETKGEIYVAFNASHLPAV 758

BLAST of CsaV3_7G028580 vs. ExPASy TrEMBL
Match: A0A0A0K551 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 SV=1)

HSP 1 Score: 1795.0 bits (4648), Expect = 0.0e+00
Identity = 885/885 (100.00%), Postives = 885/885 (100.00%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
           VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
           TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
           YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
           NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
           VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 886
           RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885

BLAST of CsaV3_7G028580 vs. ExPASy TrEMBL
Match: A0A1S3BRF8 (isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1)

HSP 1 Score: 1687.5 bits (4369), Expect = 0.0e+00
Identity = 830/884 (93.89%), Postives = 852/884 (96.38%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MATFLSSLVIQPFCV+NCGVTES KLA SDHLTYGQKTKYQ GKMDEARMLAHGE+KVGA
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
           VKSSHRNLSK Y  SGI VGKSGQRLGIGGKSKEQR+VATYLFRTEFGDLVNVFVGK GS
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
            FTVNIE+ SMQLVS DE LLLSW VYRSDSA VT NF+SSPPD TTGATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLK+PMGVDSGSSEIRSH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SGDGS+NFSIFSS+AES+VLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
           YGYQCKG KSHENQDGLEVSRIVVDPYAKILAPSIPKS  QGLG P KFLGQIS VP FD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLFVYRLNV+RFT+DKSSQLPADIAGTFSGLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVI+EVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           TS +GALQGIDDSSYYF NRVANLEE+SALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
           NASFLL+GHHGELLSRPPFVEAIAFDP+LSKTKLVADFWDPQEL SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPT+ LKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
           VAFND+RSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQ+RNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWEDASCKFLAVMLRADKEENESITENPKTRS+IFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 885
           RVVDTALPFPGFFSSDGELVPMTGSVTYE+QAHSCALFEAKSAN
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of CsaV3_7G028580 vs. ExPASy TrEMBL
Match: A0A5A7UR47 (Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 PE=4 SV=1)

HSP 1 Score: 1686.0 bits (4365), Expect = 0.0e+00
Identity = 829/884 (93.78%), Postives = 851/884 (96.27%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MATFLSSLVIQPFCV+NCGVTES KLA SDHLTYGQKTKYQ GKMDEARMLAHGE+KVGA
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
           VKSSHRNLSK Y  SGISVGKSGQRLGIGGKSKEQR+VATYLFRTEFGDLVNVFVGK GS
Sbjct: 61  VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
            FTVNIE+ SMQLVS DE LLLSW VYRSDSA VT NF+SSPPD TTGATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLK+PMGVDSGSSEIRSH+KTSFSVPVGFGRGYPSP GLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240

Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SGDGS+NFSIFSS+AES+VLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEG S FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300

Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
           YGYQCKG KSHENQDGLEVSRIVVDPYAKILAPSIPKS  QGLG P KFLGQIS VP FD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLFVYRLNV+RFT+DKSSQLPADIAGTFSGLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVI+EVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           TS +GALQGIDDSSYYF NRVANLEE+SALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
           NASFLL+GHHGELLSRPPFVEAIAFDP+LSKTKLVADFWDPQEL SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPT+ LKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
           VAFND+RSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQ+RNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWEDASCKFLAVMLRADKEENESITENPKTRS+IFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 885
           RVVDTALPFPGFFSSDGELVPMTGSVTYE+QAHSCALFEAKSAN
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of CsaV3_7G028580 vs. ExPASy TrEMBL
Match: A0A6J1GPU3 (isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456039 PE=4 SV=1)

HSP 1 Score: 1510.4 bits (3909), Expect = 0.0e+00
Identity = 736/885 (83.16%), Postives = 803/885 (90.73%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MATFLSSL IQPFC YN G TES KLA SD+ TYGQK  YQ GK+DEARM AHGE+KVG 
Sbjct: 1   MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
            KS HRN+ + YA S ISVGKSGQ+ G GGK++E R+VATYLFRTEFGDLV  FVGK+GS
Sbjct: 61  TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
            F  +IE+ S++L SIDE LLLSWGVYRS+SA+VTPNF+S  P+ET GATETPFV+TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
           KF+VELEFDAKH PFYLSF+LKYP+GVDSGSSEI+SH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SGDGS+NFSIFSS+AES+VLCLYNDSTSEKP+LELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
           YGY+C+GSK HENQD  EVSRI+VDPYAKIL  SIPK+ GQGLGLPSKFLGQI  VP F 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLF+YRLNV+ FT+DKSSQLP D AGTFSGLT+KLLHFK LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
           EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANGVEVILEVV+TH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480

Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           T+ +GALQGIDDS YYF NRVAN E KSALNCN+PIVQQLLLDSLR+WVTEFH+DGFCF+
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540

Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
           NAS LL+GHHGELLSRPPFVEAIAFDPLLSKTK++ADFWDPQE   KETRFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKF +DI++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +S LASELSWNCGEEGPTSN++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
           VAF D++SFNWD LKTDFGTQTTQFI FLSSFRSRRFDLFQ+RNFLK ENIDWFDNNQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
           P WED SCKFLAVMLRADKEE+ SITENPK +S+IFM FNASDQS SVALPEP EGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 886
           R+VDTAL FPGFFSSDGE VP T  VTY +QAHSC LFEAKS ND
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFND 884

BLAST of CsaV3_7G028580 vs. ExPASy TrEMBL
Match: A0A6J1GN63 (isoamylase 2, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456039 PE=4 SV=1)

HSP 1 Score: 1489.6 bits (3855), Expect = 0.0e+00
Identity = 730/885 (82.49%), Postives = 795/885 (89.83%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MATFLSSL IQPFC YN G TES KLA SD+ TYGQK  YQ GK+DEARM AHGE+KVG 
Sbjct: 1   MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
            KS HRN+ + YA S ISV          GKS+E R+VATYLFRTEFGDLV  FVGK+GS
Sbjct: 61  TKSLHRNVCRAYAPSRISV----------GKSEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
            F  +IE+ S++L SIDE LLLSWGVYRS+SA+VTPNF+S  P+ET GATETPFV+TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
           KF+VELEFDAKH PFYLSF+LKYP+GVDSGSSEI+SH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SGDGS+NFSIFSS+AES+VLCLYNDSTSEKP+LELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
           YGY+C+GSK HENQD  EVSRI+VDPYAKIL  SIPK+ GQGLGLPSKFLGQI  VP F 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLF+YRLNV+ FT+DKSSQLP D AGTFSGLT+KLLHFK LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
           EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANGVEVILEVV+TH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480

Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           T+ +GALQGIDDS YYF NRVAN E KSALNCN+PIVQQLLLDSLR+WVTEFH+DGFCF+
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540

Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
           NAS LL+GHHGELLSRPPFVEAIAFDPLLSKTK++ADFWDPQE   KETRFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKF +DI++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +S LASELSWNCGEEGPTSN++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
           VAF D++SFNWD LKTDFGTQTTQFI FLSSFRSRRFDLFQ+RNFLK ENIDWFDNNQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
           P WED SCKFLAVMLRADKEE+ SITENPK +S+IFM FNASDQS SVALPEP EGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 886
           R+VDTAL FPGFFSSDGE VP T  VTY +QAHSC LFEAKS ND
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFND 874

BLAST of CsaV3_7G028580 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 942.6 bits (2435), Expect = 2.3e-274
Identity = 477/881 (54.14%), Postives = 628/881 (71.28%), Query Frame = 0

Query: 14  CVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGAVKSSHRNLSKTYA 73
           C  N G+T + K   S  L  G+K K + G   E  M          V S+     ++Y 
Sbjct: 13  CCLNNGITRTWKF-PSARLFTGRKNKIKLG--SETLMFTRKRFMGDLVTSA----LQSYQ 72

Query: 74  KSGISVGKSGQRLGIGGKSK-----EQRRVATYLFRTEFGDLVNVFVGKKGSTFTVNIEV 133
            S I   K+   L     S+     + ++V +Y FRT+ G LV V V KK   +++ + V
Sbjct: 73  FSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYV 132

Query: 134 PSMQLVSIDEA-LLLSWGVYRSDSALVTP-NFESSPPDETTGATETPFVKTSEGKFSVEL 193
            S++L   D++ L++ WGVYRSDS+   P +FE+S  D  T  TET FVK+S  +  + L
Sbjct: 133 SSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGL 192

Query: 194 EFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSIS-GDGS 253
           EFD K +PFYLSF LK   G D    E+ +H+ T F +PVGF  G+P PLGLS    D S
Sbjct: 193 EFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDS 252

Query: 254 VNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGYQC 313
            NFS FS S+ ++VLCLY+DST++KP LELDLDPY+NR+G++WHAS +    FV YGY+C
Sbjct: 253 WNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRC 312

Query: 314 KGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFDWDGEV 373
           K +   +    +E   IV+DPYA ++  S+ +          K+LG +SK P+FDW  +V
Sbjct: 313 KETAHSKEDVDVEGEPIVLDPYATVVGKSVSQ----------KYLGSLSKSPSFDWGEDV 372

Query: 374 HPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQ 433
            PN+P+EKL VYRLNV+ FT  +SS+LP+++AGTFSG+ +K+ H K LG NAVLLEPIF 
Sbjct: 373 SPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFS 432

Query: 434 FDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTSGNG 493
           F E++GPYFPFHFFSP + YGPS +  SA+NSMK MVK+LH+ G+EV+LEVV+THT+ +G
Sbjct: 433 FSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG 492

Query: 494 ALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFL 553
           AL+GIDDSSYY+  R  +L+ KS LNCNYP+VQQL+L+SLRYWVTEFHVDGFCF+NAS L
Sbjct: 493 ALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSL 552

Query: 554 LRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFC 613
           LRG HGE LSRPP VEAIAFDPLL++TKL+AD WDP E+  KE RFPHWKRWAE+N+++C
Sbjct: 553 LRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYC 612

Query: 614 SDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSNLA 673
            ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN GL LVD+VSFS   LA
Sbjct: 613 RNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELA 672

Query: 674 SELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFND 733
           SELSWNCGEEG T+   VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS     
Sbjct: 673 SELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLES 732

Query: 734 KRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQWED 793
           ++ F+W+LL + FGTQ TQFI+F++S R+RR D+FQ R+FLK ENI W+ N+Q+ P+WED
Sbjct: 733 RKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWED 792

Query: 794 ASCKFLAVMLRADKEENE--SITENPKTRSN-IFMVFNASDQSESVALPEPLEGTSWFRV 853
            + KFLA+ ++++ EE E  S+ E  + +SN +F+ FNASD  ESV LP   +G+ W R+
Sbjct: 793 PASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRL 852

Query: 854 VDTALPFPGFFSSDGELV----PMTGSVTYEIQAHSCALFE 880
           VDTALPFPGFFS +GE V    P+   V YE++ +SC LFE
Sbjct: 853 VDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of CsaV3_7G028580 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 942.6 bits (2435), Expect = 2.3e-274
Identity = 477/881 (54.14%), Postives = 628/881 (71.28%), Query Frame = 0

Query: 14  CVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGAVKSSHRNLSKTYA 73
           C  N G+T + K   S  L  G+K K + G   E  M          V S+     ++Y 
Sbjct: 13  CCLNNGITRTWKF-PSARLFTGRKNKIKLG--SETLMFTRKRFMGDLVTSA----LQSYQ 72

Query: 74  KSGISVGKSGQRLGIGGKSK-----EQRRVATYLFRTEFGDLVNVFVGKKGSTFTVNIEV 133
            S I   K+   L     S+     + ++V +Y FRT+ G LV V V KK   +++ + V
Sbjct: 73  FSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYV 132

Query: 134 PSMQLVSIDEA-LLLSWGVYRSDSALVTP-NFESSPPDETTGATETPFVKTSEGKFSVEL 193
            S++L   D++ L++ WGVYRSDS+   P +FE+S  D  T  TET FVK+S  +  + L
Sbjct: 133 SSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGL 192

Query: 194 EFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSIS-GDGS 253
           EFD K +PFYLSF LK   G D    E+ +H+ T F +PVGF  G+P PLGLS    D S
Sbjct: 193 EFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDS 252

Query: 254 VNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGYQC 313
            NFS FS S+ ++VLCLY+DST++KP LELDLDPY+NR+G++WHAS +    FV YGY+C
Sbjct: 253 WNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRC 312

Query: 314 KGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFDWDGEV 373
           K +   +    +E   IV+DPYA ++  S+ +          K+LG +SK P+FDW  +V
Sbjct: 313 KETAHSKEDVDVEGEPIVLDPYATVVGKSVSQ----------KYLGSLSKSPSFDWGEDV 372

Query: 374 HPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQ 433
            PN+P+EKL VYRLNV+ FT  +SS+LP+++AGTFSG+ +K+ H K LG NAVLLEPIF 
Sbjct: 373 SPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFS 432

Query: 434 FDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTSGNG 493
           F E++GPYFPFHFFSP + YGPS +  SA+NSMK MVK+LH+ G+EV+LEVV+THT+ +G
Sbjct: 433 FSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG 492

Query: 494 ALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFL 553
           AL+GIDDSSYY+  R  +L+ KS LNCNYP+VQQL+L+SLRYWVTEFHVDGFCF+NAS L
Sbjct: 493 ALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSL 552

Query: 554 LRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFC 613
           LRG HGE LSRPP VEAIAFDPLL++TKL+AD WDP E+  KE RFPHWKRWAE+N+++C
Sbjct: 553 LRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYC 612

Query: 614 SDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSNLA 673
            ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN GL LVD+VSFS   LA
Sbjct: 613 RNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELA 672

Query: 674 SELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFND 733
           SELSWNCGEEG T+   VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS     
Sbjct: 673 SELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLES 732

Query: 734 KRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQWED 793
           ++ F+W+LL + FGTQ TQFI+F++S R+RR D+FQ R+FLK ENI W+ N+Q+ P+WED
Sbjct: 733 RKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWED 792

Query: 794 ASCKFLAVMLRADKEENE--SITENPKTRSN-IFMVFNASDQSESVALPEPLEGTSWFRV 853
            + KFLA+ ++++ EE E  S+ E  + +SN +F+ FNASD  ESV LP   +G+ W R+
Sbjct: 793 PASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRL 852

Query: 854 VDTALPFPGFFSSDGELV----PMTGSVTYEIQAHSCALFE 880
           VDTALPFPGFFS +GE V    P+   V YE++ +SC LFE
Sbjct: 853 VDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of CsaV3_7G028580 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 374.0 bits (959), Expect = 3.3e-103
Identity = 246/701 (35.09%), Postives = 360/701 (51.36%), Query Frame = 0

Query: 231 GYPSPLGLSISGDGSVNFSIFSSSAESLVLCLY-----NDSTSEKPLLELDLDPYINRSG 290
           G  SPLG+S   D  +NF++FS +A S+ LCL       D T +  ++EL LDP +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 291 NIWHASFEGAS-KFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLG 350
           + WH   E      V YGY+  G    +     + S +++DPYAK++      S G    
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGH--SSFGDSSQ 207

Query: 351 LPSKFLGQIS-KVPTFDW-DGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSG 410
             ++F G    +   FDW D    PN+P + L +Y +NV  FT D+SS +   I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267

Query: 411 LTKKLLHFKNLGVNAVLLEPIFQFDEKE--------------GPYFPFHFFSPTNNYGP- 470
             +K+ H ++LG+NAV L P+F+FDE E                Y   +FF+P + Y   
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327

Query: 471 SGASISAINSMKEMVKELHANGVEVILEVVYTHTSG-------NGALQGIDDSSYYF--- 530
            G  I A    KEMVK LH+ G+EVIL+VVY HT+          + +GID+  YY    
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387

Query: 531 TNRVANLEE-KSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRGHHGELLSR 590
            N++ N     + LNCN+P+V +L+LDSLR+WVTE+HVDGF F  AS L R   G  LS 
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447

Query: 591 PPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFCSDIRDFFRGE- 650
           PP + AIA D +LS+ K++A+ WD   L     +FP+W RWAE N  +  D+R F +G+ 
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGL-YLVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507

Query: 651 GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFS-----------NSN 710
           G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++           N  
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567

Query: 711 LASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSS-GGSVA 770
                SWNCG EG T +  +   R +Q++NF   L +S G P++ MGDE G +  G + +
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627

Query: 771 FNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRR-FDLFQNRNFLKGENIDWFDNNQSPP 830
           +    S N    K     +   F  F    + R    + ++ NFL    I W ++N    
Sbjct: 628 YGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDN---- 687

Query: 831 QWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWFR 882
            W+++  KFLA  L      ++ I        +I++ FNA D      +P+P  G  WFR
Sbjct: 688 -WDNSESKFLAFTL------HDGIGGR-----DIYVAFNAHDYFVKALIPQPPPGKQWFR 747

BLAST of CsaV3_7G028580 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 352.1 bits (902), Expect = 1.3e-96
Identity = 242/690 (35.07%), Postives = 345/690 (50.00%), Query Frame = 0

Query: 231 GYPSPLGLSISGDGSVNFSIFSSSAESLVLCL--YNDSTSEKPLLELDLDPYINRSGNIW 290
           G PSP G ++  DG VNFS++S+++ S  +CL   +D    K   E+ LDP  NR+G++W
Sbjct: 72  GLPSPFGPTVRDDG-VNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVW 131

Query: 291 HASFEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSK 350
           H    G  K + YGY+  G  S E     + S I++DPYAK +   I +     LG    
Sbjct: 132 HVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAI---ISRDEFGVLGPDDN 191

Query: 351 FLGQIS-KVPT----FDWDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGL 410
              Q++  VPT    FDW+G++H  LP + L +Y ++V  FT  +SS++  +  GT+ G+
Sbjct: 192 CWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGV 251

Query: 411 TKKLLHFKNLGVNAVLLEPIFQFDEKE---------------GPYFPFHFFSPTNNYGPS 470
            +KL H K LG+N + L P  +F+E E                 Y    FFSP   Y  +
Sbjct: 252 AEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASA 311

Query: 471 GASI---SAINSMKEMVKELHANGVEVILEVVYTHTS-GNG-----ALQGIDDSSYYFTN 530
            ++     AIN  K +VKE H  G+EVI++VV  HT+ GN      + +G+D+S YY   
Sbjct: 312 SSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLA 371

Query: 531 RVANLEEKS----ALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRGH------ 590
                   S      NCN+P+V+Q +LD LRYWVTE HVDGF F   S + R        
Sbjct: 372 PKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAA 431

Query: 591 -------HGELL------SRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKR 650
                   G+LL      S PP ++ I+ DP+L   KL+A+ WD   L  +   FPHW  
Sbjct: 432 NVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGL-YQVGMFPHWGI 491

Query: 651 WAEVNSKFCSDIRDFFRG-EGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVD 710
           W+E N KF   +R F +G +G   + A  LCGS +++  GR P  S NFI  + G  L D
Sbjct: 492 WSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLAD 551

Query: 711 LVSFSN-SNLAS----------ELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGV 770
           LV+++N +NLA+            SWNCGEEG  +++ V   R +Q+RNF   L VS GV
Sbjct: 552 LVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGV 611

Query: 771 PVLNMGDECGQSSGGS----VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLF 830
           P++ MGDE G + GG+       N    F WD  K +  +   +F   L  FR     L 
Sbjct: 612 PMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWD-KKEEAHSDFFRFCRILIKFRDECESLG 671

Query: 831 QNRNFLKGENIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFN 851
            N +F   + + W       P W + S +F+A  L  D  + E           I++ FN
Sbjct: 672 LN-DFPTAKRLQWHGLAPEIPNWSETS-RFVAFSL-VDSVKKE-----------IYVAFN 731

BLAST of CsaV3_7G028580 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 69.7 bits (169), Expect = 1.3e-11
Identity = 86/366 (23.50%), Postives = 144/366 (39.34%), Query Frame = 0

Query: 232 YPSPLGLSISGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHAS 291
           Y  PLG   + +G V+  +++ +A+++ +C+Y +   + P   +++ P +  +  +W  S
Sbjct: 204 YDGPLGAHFTPEG-VSLHLWAPTAQAVSVCIYKNPLDKSP---MEICP-LKEANGVW--S 263

Query: 292 FEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLG 351
            EGA  +    Y  K S  H +   LE      DPYA+ L+    K+          FL 
Sbjct: 264 TEGACSWGGCYYVYKVSVYHPSTMKLETC-YANDPYARGLSADGRKT----------FLV 323

Query: 352 QISK--VPTFDWD--GEVHPNL-PMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKK 411
            +    +    WD   +  P L     + +Y L+V  F+ +  +  P +  G  +  +K 
Sbjct: 324 NLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTSKD 383

Query: 412 LLHFKNL------GVNAVLLEPIFQF----DEKEG------------------------- 471
               K+L      G+  + L P FQF    DEKE                          
Sbjct: 384 SAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARITE 443

Query: 472 ---------PYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTS 531
                     Y P  +  P  +Y         I   ++MV+ L+  G+ V+L+VVY H  
Sbjct: 444 IQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTGLNVVLDVVYNHLH 503

Query: 532 GNG------ALQGIDDSSYYFTNRVANLEEKSALN---CNYPIVQQLLLDSLRYWVTEFH 540
            +G       L  I    Y   N    +E  + +N     + +V +L+ D L  WV  + 
Sbjct: 504 ASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNYK 551