CsaV3_7G025280 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_7G025280 vs. NCBI nr
Match: XP_011659150.1 (trans-resveratrol di-O-methyltransferase [Cucumis sativus] >KGN44537.1 hypothetical protein Csa_016151 [Cucumis sativus])

HSP 1 Score: 762.3 bits (1967), Expect = 2.0e-216
Identity = 378/378 (100.00%), Postives = 378/378 (100.00%), Query Frame = 0

Query: 1   MESRNTQMEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLI 60
           MESRNTQMEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLI
Sbjct: 1   MESRNTQMEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLI 60

Query: 61  SALQIHPQKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQ 120
           SALQIHPQKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQ
Sbjct: 61  SALQIHPQKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQ 120

Query: 121 HNPLTLSPFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEG 180
           HNPLTLSPFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEG
Sbjct: 121 HNPLTLSPFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEG 180

Query: 181 FNAAMESDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQ 240
           FNAAMESDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQ
Sbjct: 181 FNAAMESDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQ 240

Query: 241 VVAGLEGNHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNG 300
           VVAGLEGNHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNG
Sbjct: 241 VVAGLEGNHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNG 300

Query: 301 KVIIIDMVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFG 360
           KVIIIDMVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFG
Sbjct: 301 KVIIIDMVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFG 360

Query: 361 AYNISPILGLRSLIELYP 379
           AYNISPILGLRSLIELYP
Sbjct: 361 AYNISPILGLRSLIELYP 378

BLAST of CsaV3_7G025280 vs. NCBI nr
Match: XP_038899266.1 (trans-resveratrol di-O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 627.5 bits (1617), Expect = 7.6e-176
Identity = 313/378 (82.80%), Postives = 340/378 (89.95%), Query Frame = 0

Query: 1   MESRNTQMEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLI 60
           ME RNT+M M+GEE+L+AQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQL+
Sbjct: 1   MEGRNTKMGMEGEEMLRAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLL 60

Query: 61  SALQIHPQKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQ 120
           S+LQIHP+KSQHV RIMRIL HSGFFLIHNK +QQ DDD+D++E   AYSLTNPSLLLLQ
Sbjct: 61  SSLQIHPRKSQHVRRIMRILVHSGFFLIHNKTTQQGDDDQDQEE---AYSLTNPSLLLLQ 120

Query: 121 HNPLTLSPFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEG 180
           HNPLTLSPF LFMLDRVLIEPW F+SAWL +D+D RTPFE A+GK+FWE+MGSE KDGEG
Sbjct: 121 HNPLTLSPFLLFMLDRVLIEPWQFVSAWLLNDTDNRTPFEMAYGKKFWEYMGSETKDGEG 180

Query: 181 FNAAMESDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQ 240
           FNAAM SD +LV+SVLLEKYKSVFEGVE  VDVGGGTG+VAK IAEAFPQ+KCVV+DLPQ
Sbjct: 181 FNAAMGSDGRLVMSVLLEKYKSVFEGVECLVDVGGGTGTVAKIIAEAFPQIKCVVFDLPQ 240

Query: 241 VVAGLEGNHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNG 300
           VV GLEGNHNLMFLQGDMFQ IP ADALLLKWILHDWTD+EC+KILKKCKEAITSNGK G
Sbjct: 241 VVDGLEGNHNLMFLQGDMFQAIPPADALLLKWILHDWTDEECIKILKKCKEAITSNGKIG 300

Query: 301 KVIIIDMVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFG 360
           KVIIIDMVV   +N K MK ND LM + K FWDLLMMVNVGGKERDEKEWA+LFQ AGF 
Sbjct: 301 KVIIIDMVV---TNNKKMK-NDKLMIECKFFWDLLMMVNVGGKERDEKEWAQLFQLAGFS 360

Query: 361 AYNISPILGLRSLIELYP 379
            YNI PILGLRSLIELYP
Sbjct: 361 TYNIFPILGLRSLIELYP 371

BLAST of CsaV3_7G025280 vs. NCBI nr
Match: XP_038899253.1 (trans-resveratrol di-O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 561.6 bits (1446), Expect = 5.1e-156
Identity = 280/379 (73.88%), Postives = 322/379 (84.96%), Query Frame = 0

Query: 1   MESRNTQMEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLI 60
           ME  NT+M M+ EEVLKAQAHIWNHIFSFINSMALKCA+ELRIPDAI+RHGSPITLSQL+
Sbjct: 1   MEGTNTKMGMEEEEVLKAQAHIWNHIFSFINSMALKCAIELRIPDAISRHGSPITLSQLL 60

Query: 61  SALQIHPQKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQ 120
           S+LQIHP+KSQHV RIMRILAHSGFFLI NK +QQ DDD+D++E   AYSLTN S+LLLQ
Sbjct: 61  SSLQIHPRKSQHVRRIMRILAHSGFFLIQNKTTQQGDDDQDQEE---AYSLTNASILLLQ 120

Query: 121 HNPLTLSPFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEG 180
           HNPLTLSP+ LF+L+  ++ PW  +S W RS+   R+PFETA+GK+FWE+M SE +DGE 
Sbjct: 121 HNPLTLSPYLLFVLNHSILHPWQSLSVWFRSNGLDRSPFETAYGKKFWEYMESETRDGEA 180

Query: 181 FNAAMESDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQ 240
           FN +M  D+KLV+SVLL  YKS+FEGVESFVDVGGGTG++AKA+AEAFPQMKC V+DLPQ
Sbjct: 181 FNESMARDSKLVMSVLLGNYKSMFEGVESFVDVGGGTGTLAKAMAEAFPQMKCFVFDLPQ 240

Query: 241 VVAGLEGNHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNG-KN 300
           VVAGLEGNHNLMFLQGDMFQ IP ADALLLKWILH+W D+ECVKILKKCKEAITSN  K 
Sbjct: 241 VVAGLEGNHNLMFLQGDMFQAIPPADALLLKWILHNWNDEECVKILKKCKEAITSNKLKK 300

Query: 301 GKVIIIDMVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGF 360
           GKVIIIDMVV N +N  N K  D    + +LFWD++MM  VGG ERDE+EWA+LF  AGF
Sbjct: 301 GKVIIIDMVVTNNNN--NNKKADYYSIQTQLFWDMVMMAAVGGMERDEQEWAQLFHKAGF 360

Query: 361 GAYNISPILGLRSLIELYP 379
            +YNI PILGLRSLIELYP
Sbjct: 361 ASYNIFPILGLRSLIELYP 374

BLAST of CsaV3_7G025280 vs. NCBI nr
Match: XP_031744374.1 (probable O-methyltransferase 3 [Cucumis sativus])

HSP 1 Score: 547.0 bits (1408), Expect = 1.3e-151
Identity = 270/372 (72.58%), Postives = 315/372 (84.68%), Query Frame = 0

Query: 8   MEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLISALQIHP 67
           MEM+GEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLS+L+SA++IHP
Sbjct: 1   MEMEGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSKLLSAIEIHP 60

Query: 68  QKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQHNPLTLS 127
            KS+H+HR+MRILAHSGFFLIH K +QQ+++D+ + E  VAYSLTNPSLLLLQHNPLT S
Sbjct: 61  NKSEHIHRLMRILAHSGFFLIHKKTTQQEENDDADQE--VAYSLTNPSLLLLQHNPLTTS 120

Query: 128 PFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEGFNAAMES 187
           PF L +L++ L+ PW  +S W RS    RTPFET +GK+FWE+M SE +D E F   M S
Sbjct: 121 PFLLSVLNQSLLHPWHSLSTWFRSSGRDRTPFETTYGKKFWEYMESETRDREAFGEGMGS 180

Query: 188 DAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVAGLEG 247
           DA+LV+SVLL KYKS+FEGVESFVDVGGGTG++AKA+AEAFPQMKCVV+DLPQVVAGLEG
Sbjct: 181 DARLVISVLLGKYKSMFEGVESFVDVGGGTGTLAKAMAEAFPQMKCVVFDLPQVVAGLEG 240

Query: 248 NHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGK-NGKVIIID 307
           NHNL F QGDMFQ IPSA+ LLLKWILH+W+DDECV+ILKKCKEAI SN K   KVIIID
Sbjct: 241 NHNLTFRQGDMFQAIPSAEVLLLKWILHNWSDDECVQILKKCKEAIRSNNKLKEKVIIID 300

Query: 308 MVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFGAYNISP 367
           MV+ + S+       D + T+ +L WD+LMM +VGGKERDEKEWA+LF  AGFG+Y I P
Sbjct: 301 MVLPSSSSNNKA---DYISTQTQLLWDMLMMASVGGKERDEKEWAQLFHKAGFGSYKIFP 360

Query: 368 ILGLRSLIELYP 379
           ILGLRSLI+LYP
Sbjct: 361 ILGLRSLIKLYP 367

BLAST of CsaV3_7G025280 vs. NCBI nr
Match: XP_023548392.1 (probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 544.3 bits (1401), Expect = 8.5e-151
Identity = 270/372 (72.58%), Postives = 316/372 (84.95%), Query Frame = 0

Query: 7   QMEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLISALQIH 66
           +M M GEE+L AQAHIWNHIF+FINSMALKCAV+LRIPDAI+ HG P+TL QL+S+LQ+ 
Sbjct: 11  EMAMKGEELLNAQAHIWNHIFNFINSMALKCAVQLRIPDAISHHGQPMTLPQLVSSLQVD 70

Query: 67  PQKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQHNPLTL 126
           PQKSQH+HR+MRILA++GFFLIH       +  ++ D+ +VAYSLTN SLLLL+HNPLTL
Sbjct: 71  PQKSQHIHRLMRILAYAGFFLIH-------EPSKEGDDQEVAYSLTNSSLLLLEHNPLTL 130

Query: 127 SPFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEGFNAAME 186
           SPF L MLD VL++PW FIS WLRSD D RTPFETAHGK+FWEHMG + KDG  FNAAM 
Sbjct: 131 SPFLLSMLDPVLVDPWQFISTWLRSDRD-RTPFETAHGKQFWEHMGGKPKDGMVFNAAMA 190

Query: 187 SDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVAGLE 246
           SDA+LV SVLL KYKSVFEGVE  VDVGGGTG+V +AIAEAFPQM+CVV+DLPQVVA L+
Sbjct: 191 SDARLVTSVLLGKYKSVFEGVELLVDVGGGTGTVGQAIAEAFPQMQCVVFDLPQVVADLK 250

Query: 247 GNHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNGKVIIID 306
           G+HNLMF++GDMFQ IP A A+LLKWILHDW+D+ECVKILK+CKEAITSNGK GKV+IID
Sbjct: 251 GSHNLMFVEGDMFQQIPPAGAILLKWILHDWSDEECVKILKRCKEAITSNGKKGKVMIID 310

Query: 307 MVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFGAYNISP 366
           +VV ++  K     NDML T+ +LFWD+LMMV VGG+ER+EKEWAELF  AGFG YNI P
Sbjct: 311 LVVRDEDIK-----NDML-TETQLFWDVLMMVTVGGRERNEKEWAELFYKAGFGGYNIFP 368

Query: 367 ILGLRSLIELYP 379
           ILGLRSLIE+YP
Sbjct: 371 ILGLRSLIEIYP 368

BLAST of CsaV3_7G025280 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 2.9e-109
Identity = 207/371 (55.80%), Postives = 270/371 (72.78%), Query Frame = 0

Query: 9   EMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLISALQIHPQ 68
           E +  E+L AQAHIWN IF+FINSM+LKCA++L IPD IN HG P+T+SQL  AL I+ +
Sbjct: 20  EENSTEILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHGKPMTISQLTLALPINRK 79

Query: 69  KSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQHNPLTLSP 128
           KS  V+R+MRIL HSGFF +     Q+ +  E+   ++  Y +T+ S LLL+ NP++++P
Sbjct: 80  KSPCVYRLMRILIHSGFFAL-----QKAEVGEEGGGEEEGYVITDASKLLLKDNPMSVTP 139

Query: 129 FSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEGFNAAMESD 188
           F L MLD V+ +PW F+S W ++     TPF+TA+G  FW++   E K    FN AM SD
Sbjct: 140 FLLAMLDPVMTKPWDFLSNWFQNGDP--TPFDTANGMAFWDYGSHEPKLARFFNDAMASD 199

Query: 189 AKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVAGLEGN 248
           A+LV SV++EK K VFEGVES VDVGGGTG+VA +IA AFP ++C V+DLP VVA L+G 
Sbjct: 200 ARLVTSVVIEKCKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLPHVVADLQGG 259

Query: 249 HNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAIT-SNGKNGKVIIIDM 308
           +NL F+ GDMF  +P+ + +LLKWILHDW D+E VKILKKCKEAI+ SN K GKVIIIDM
Sbjct: 260 NNLNFVGGDMFVDVPATEVVLLKWILHDWNDEESVKILKKCKEAISKSNKKGGKVIIIDM 319

Query: 309 VVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFGAYNISPI 368
            V      +N K+ D    + +LF+D+LMM  V G+ER+EKEWA+LF+ AGF  Y I+PI
Sbjct: 320 KV------ENEKDEDDESYETQLFFDMLMMALVTGRERNEKEWAKLFKDAGFSNYKITPI 377

Query: 369 LGLRSLIELYP 379
           LGLRSLIE+YP
Sbjct: 380 LGLRSLIEVYP 377

BLAST of CsaV3_7G025280 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 388.3 bits (996), Expect = 1.0e-106
Identity = 196/366 (53.55%), Postives = 260/366 (71.04%), Query Frame = 0

Query: 14  EVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLISALQIHPQKSQHV 73
           E+L AQAH+WNHIF+FI SM+LKCA++L IPD I+ HG P+TL +L++ L +HP++SQ V
Sbjct: 11  ELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHGKPMTLPELVAKLPVHPKRSQCV 70

Query: 74  HRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQHNPLTLSPFSLFM 133
           +R+MRIL HSG FL   ++ Q K+++         Y LT+ S LLL  + L++ P  L M
Sbjct: 71  YRLMRILVHSG-FLAAQRVQQGKEEE--------GYVLTDASRLLLMDDSLSIRPLVLAM 130

Query: 134 LDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEGFNAAMESDAKLVV 193
           LD +L +PW ++SAW ++D    TPF TA+ + FW++ G E +    FN AM SDA+L+ 
Sbjct: 131 LDPILTKPWHYLSAWFQNDDP--TPFHTAYERSFWDYAGHEPQLNNSFNEAMASDARLLT 190

Query: 194 SVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVAGLEGNHNLMF 253
           SVLL++ + VF G+ S VDVGGGTG VAKAIA AFP + C V DL  VVAGL+G+ NL +
Sbjct: 191 SVLLKEGQGVFAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQGSKNLNY 250

Query: 254 LQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNGKVIIIDMVVGNKS 313
             GDMF+ IP ADA+LLKWILHDW+++ECVKILK+C+EAI S    GKVIIIDM++    
Sbjct: 251 FAGDMFEAIPPADAILLKWILHDWSNEECVKILKRCREAIPSKENGGKVIIIDMIM---- 310

Query: 314 NKKNMKN-NDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFGAYNISPILGLRS 373
               MKN  D   T+ +LF+D+ MM+   G+ERDE EW +LF  AGF  Y I+PILGLRS
Sbjct: 311 ----MKNQGDYKSTETQLFFDMTMMIFAPGRERDENEWEKLFLDAGFSHYKITPILGLRS 357

Query: 374 LIELYP 379
           LIE+YP
Sbjct: 371 LIEVYP 357

BLAST of CsaV3_7G025280 vs. ExPASy Swiss-Prot
Match: Q6VMW0 (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 6.6e-98
Identity = 188/368 (51.09%), Postives = 259/368 (70.38%), Query Frame = 0

Query: 13  EEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLISALQIHPQKSQH 72
           +E+L+AQAH+WNHI+S+INSM+LKCA++L IPDAI++HG+PITLSQL  AL I+  KS  
Sbjct: 11  QELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHGNPITLSQLADALNINKAKSHG 70

Query: 73  VHRIMRILAHSGFFLIHNKLS-QQKDDDEDEDEDQVAYSLTNPSLLLLQHNPLTLSPFSL 132
           + R+MRIL HSGFF   +K+  + K + EDE+E++ AYSLT  S LLL+  PL+++PF+L
Sbjct: 71  LFRLMRILVHSGFF---DKVKVKVKVEGEDEEEEEDAYSLTPASRLLLRSEPLSVAPFAL 130

Query: 133 FMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEGFNAAMESDAKL 192
            M D V  E W  +S W R+D+     F+T +G  F E+  ++ +    FN AM  DA  
Sbjct: 131 AMSDPVYTETWHHLSEWFRNDA--VAAFDTKYGMTFPEYAVADDRLNVLFNEAMACDAGF 190

Query: 193 VVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVAGLEGNHNL 252
           V S+L  + + +F+G+ES VDVGGGTG+ AK IA AFP M+C V DLP VV GL+G+ NL
Sbjct: 191 VNSILTTECREIFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLDLPNVVGGLKGSENL 250

Query: 253 MFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAIT-SNGKNGKVIIIDMVVG 312
            F+ GDMF  IP ADA+ +K+ILHDW D+ECVKILKKCKEAI+ SN    K+I++++V+ 
Sbjct: 251 SFVSGDMFDFIPHADAIFMKFILHDWNDEECVKILKKCKEAISRSNNSCRKIILVEIVME 310

Query: 313 NKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFGAYNISPILGL 372
           ++             T+ KLF+D+ M+  + GKER EKEW +LF  AGF  Y I+ +LGL
Sbjct: 311 DEKETHE-------ATETKLFFDMQMLAIITGKERSEKEWGKLFFDAGFTNYKITRVLGL 366

Query: 373 RSLIELYP 379
           RS+IE++P
Sbjct: 371 RSVIEVFP 366

BLAST of CsaV3_7G025280 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 8.6e-98
Identity = 188/368 (51.09%), Postives = 255/368 (69.29%), Query Frame = 0

Query: 12  GEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRH-GSPITLSQLISALQIHPQKS 71
           GEE+L AQAHI+NHIF+FI+SMALKCAVEL IP+ ++ H    +TLS+L+ ALQI   KS
Sbjct: 21  GEELLAAQAHIYNHIFNFISSMALKCAVELNIPEILHNHQPKAVTLSELVQALQIPQAKS 80

Query: 72  QHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQHNPLTLSPFS 131
             ++R++RIL HSGFF I  K+  + D++         Y  T  S LLL+++P+++SP  
Sbjct: 81  ACLYRLLRILVHSGFFAI-TKIQSEGDEE--------GYLPTLSSKLLLKNHPMSMSPCL 140

Query: 132 LFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEGFNAAMESDAK 191
           L +++  ++ P  F S W +  SD  TPFE  HG   W++ G      E FN AM  + +
Sbjct: 141 LGLVNPTMVAPMHFFSDWFKR-SDDMTPFEATHGASLWKYFGETPHMAEIFNEAMGCETR 200

Query: 192 LVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVAGLEGNHN 251
           L +SV+L++ K   EG+ S VDVGGGTG+V +AIAEAFP +KC V DLPQVV  L+G++N
Sbjct: 201 LAMSVVLKECKGKLEGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLPQVVGNLKGSNN 260

Query: 252 LMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNGKVIIIDMVVG 311
           L F+ GDMFQ IP AD + LKWILHDW D+EC+KILK+CKEAI S  + GK+IIIDMVV 
Sbjct: 261 LEFVSGDMFQFIPPADVVFLKWILHDWNDEECIKILKRCKEAIPSKEEGGKLIIIDMVV- 320

Query: 312 NKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFGAYNISPILGL 371
           N  NK + ++     T+ +LF+DL +M  + G ER E EW +LF AAGF +Y ISP+LGL
Sbjct: 321 NDHNKGSYES-----TETQLFYDLTLMALLTGTERTETEWKKLFVAAGFTSYIISPVLGL 372

Query: 372 RSLIELYP 379
           +S+IE++P
Sbjct: 381 KSIIEVFP 372

BLAST of CsaV3_7G025280 vs. ExPASy Swiss-Prot
Match: B0EXJ8 (Tabersonine 16-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=16OMT PE=1 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 2.2e-93
Identity = 188/374 (50.27%), Postives = 247/374 (66.04%), Query Frame = 0

Query: 8   MEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLISAL--QI 67
           M++  EE   AQA IW+   SFI S +LKCAV+L IPD I+ HG PITLS+L +AL   +
Sbjct: 1   MDVQSEEFRGAQAQIWSQSCSFITSASLKCAVKLGIPDTIDNHGKPITLSELTNALVPPV 60

Query: 68  HPQKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQHNPLT 127
           HP K+  ++R+MR+LA +GF       S+++ D E E      YSLT  S +LL+  PL 
Sbjct: 61  HPSKAPFIYRLMRVLAKNGF------CSEEQLDGETEP----LYSLTPSSRILLKKEPLN 120

Query: 128 LSPFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEGFNAAM 187
           L    L M D V ++ W  +S W +++ D  T FETAHGK FW +     +  E FN AM
Sbjct: 121 LRGIVLTMADPVQLKAWESLSDWYQNEDDSSTAFETAHGKNFWGYSSEHMEHAEFFNEAM 180

Query: 188 ESDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVAGL 247
            SD++L+  +L+ +YK +FEG+ S VD+GGGTG++AKAIA+ FPQ+KC V+DLP VVA L
Sbjct: 181 ASDSQLISKLLIGEYKFLFEGLASLVDIGGGTGTIAKAIAKNFPQLKCTVFDLPHVVANL 240

Query: 248 EGNHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNGKVIII 307
           E   N+ F+ GDMF+ IPSA+A+ LKWILHDW D++CVKILK CK+AI + G  GKVIII
Sbjct: 241 ESKENVEFVAGDMFEKIPSANAIFLKWILHDWNDEDCVKILKSCKKAIPAKG--GKVIII 300

Query: 308 DMVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFGAYNIS 367
           DMV+   S+KK     D  + K +   D+ M+VN   KER EKEWA LF+ AGF  Y I 
Sbjct: 301 DMVM--YSDKK-----DDHLVKTQTSMDMAMLVNFAAKERCEKEWAFLFKEAGFSDYKIY 355

Query: 368 PILGL-RSLIELYP 379
           P L   RSLIE+YP
Sbjct: 361 PKLDFTRSLIEVYP 355

BLAST of CsaV3_7G025280 vs. ExPASy TrEMBL
Match: A0A0A0K881 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G328250 PE=3 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 9.6e-217
Identity = 378/378 (100.00%), Postives = 378/378 (100.00%), Query Frame = 0

Query: 1   MESRNTQMEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLI 60
           MESRNTQMEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLI
Sbjct: 1   MESRNTQMEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLI 60

Query: 61  SALQIHPQKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQ 120
           SALQIHPQKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQ
Sbjct: 61  SALQIHPQKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQ 120

Query: 121 HNPLTLSPFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEG 180
           HNPLTLSPFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEG
Sbjct: 121 HNPLTLSPFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEG 180

Query: 181 FNAAMESDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQ 240
           FNAAMESDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQ
Sbjct: 181 FNAAMESDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQ 240

Query: 241 VVAGLEGNHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNG 300
           VVAGLEGNHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNG
Sbjct: 241 VVAGLEGNHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNG 300

Query: 301 KVIIIDMVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFG 360
           KVIIIDMVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFG
Sbjct: 301 KVIIIDMVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFG 360

Query: 361 AYNISPILGLRSLIELYP 379
           AYNISPILGLRSLIELYP
Sbjct: 361 AYNISPILGLRSLIELYP 378

BLAST of CsaV3_7G025280 vs. ExPASy TrEMBL
Match: A0A6J1EPC7 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111436348 PE=3 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.7e-150
Identity = 273/371 (73.58%), Postives = 314/371 (84.64%), Query Frame = 0

Query: 8   MEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLISALQIHP 67
           M M GEE+LKAQAHIWNHIF+FINSMALKCAV+LRIPDAI+ HG PITLSQL+S+LQ+ P
Sbjct: 1   MAMKGEELLKAQAHIWNHIFNFINSMALKCAVQLRIPDAISHHGQPITLSQLVSSLQVDP 60

Query: 68  QKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQHNPLTLS 127
           QKSQH+HR+MRILA++GFFLIH + S+Q DD E      VAYSLTN SLLLL+HN L LS
Sbjct: 61  QKSQHIHRLMRILAYAGFFLIH-ETSKQGDDQE------VAYSLTNSSLLLLEHNTLALS 120

Query: 128 PFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEGFNAAMES 187
           PF L MLD VL++PW FIS WLR+D D RTPFETAHGK+FWEHMG E KDG  F AAM S
Sbjct: 121 PFLLSMLDPVLVDPWQFISTWLRNDRD-RTPFETAHGKQFWEHMGGELKDGMVFIAAMAS 180

Query: 188 DAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVAGLEG 247
           DA+LV SVLL  YKSVFEGVE  VDVGGGTG+VA+AIAEAFPQM+CVV+DLPQVV  L+G
Sbjct: 181 DARLVTSVLLGTYKSVFEGVELLVDVGGGTGTVAQAIAEAFPQMQCVVFDLPQVVGDLKG 240

Query: 248 NHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNGKVIIIDM 307
           + NL+F++GDMFQ IP ADALL+KWILHDW+D+EC KILKKCKEAITSNGK GKV+IID+
Sbjct: 241 SQNLIFVEGDMFQQIPPADALLMKWILHDWSDEECAKILKKCKEAITSNGKKGKVMIIDL 300

Query: 308 VVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFGAYNISPI 367
           VV ++ NK     NDML T+ +LFWD+LMMV VGG+ER+EKEWAELF  AGFG YNI PI
Sbjct: 301 VVRDEDNK-----NDML-TETQLFWDVLMMVTVGGRERNEKEWAELFYKAGFGGYNIFPI 357

Query: 368 LGLRSLIELYP 379
           LGLRSLIE+YP
Sbjct: 361 LGLRSLIEIYP 357

BLAST of CsaV3_7G025280 vs. ExPASy TrEMBL
Match: A0A6J1JX64 (probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111488285 PE=3 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.7e-149
Identity = 266/378 (70.37%), Postives = 316/378 (83.60%), Query Frame = 0

Query: 1   MESRNTQMEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLI 60
           +  +  +M M GEE+LKAQAHIWNHIF+FINSMALKCAV+LRIPDAI+ HG P+TL QL+
Sbjct: 39  LSKKGIEMAMKGEELLKAQAHIWNHIFNFINSMALKCAVQLRIPDAISHHGQPMTLPQLV 98

Query: 61  SALQIHPQKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQ 120
           S+LQ+ PQKSQH+HR+MRILA++GFFLIH       +  ++ D+ +VAYSLTN SLLLL+
Sbjct: 99  SSLQVDPQKSQHIHRLMRILAYAGFFLIH-------EPSKEGDDQEVAYSLTNSSLLLLE 158

Query: 121 HNPLTLSPFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEG 180
           HNP TLSPF L MLD VL++PW FIS WLR+D D RTPFETAHGK+FWEHMG + KDG  
Sbjct: 159 HNPFTLSPFLLSMLDPVLVDPWKFISTWLRNDCD-RTPFETAHGKQFWEHMGGKPKDGMV 218

Query: 181 FNAAMESDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQ 240
           FNAAM SD++LV SVLL K+KSVFEGVE  VDVGGGTG+VA+AIAEAFPQM+CVV+DLPQ
Sbjct: 219 FNAAMASDSRLVTSVLLGKHKSVFEGVELLVDVGGGTGTVAQAIAEAFPQMQCVVFDLPQ 278

Query: 241 VVAGLEGNHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNG 300
           VV  L+G+HNL F +GDMFQ IP ADALLLKWILHDW+D+ECV+ILK+CKEAITSNGK G
Sbjct: 279 VVGDLKGSHNLTFAEGDMFQQIPPADALLLKWILHDWSDEECVEILKRCKEAITSNGKKG 338

Query: 301 KVIIIDMVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFG 360
           KV+IID+VV ++ NK     NDML T+ +LFWD LMM+ VGG+ER+EKEWAELF  AGFG
Sbjct: 339 KVMIIDLVVRDEDNK-----NDML-TETRLFWDELMMMTVGGRERNEKEWAELFYKAGFG 398

Query: 361 AYNISPILGLRSLIELYP 379
            Y I PILGLRSLIE+YP
Sbjct: 399 GYTIFPILGLRSLIEIYP 402

BLAST of CsaV3_7G025280 vs. ExPASy TrEMBL
Match: A0A0A0K516 (Methyltransf_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G328260 PE=3 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 7.5e-137
Identity = 251/372 (67.47%), Postives = 295/372 (79.30%), Query Frame = 0

Query: 8   MEMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLISALQIHP 67
           MEM+GEEVLKAQAHIWNHIFSFINSMALKCA                    L+SA++IHP
Sbjct: 1   MEMEGEEVLKAQAHIWNHIFSFINSMALKCA--------------------LLSAIEIHP 60

Query: 68  QKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQHNPLTLS 127
            KS+H+HR+MRILAHSGFFLIH K +QQ+++D+ + E  VAYSLTNPSLLLLQHNPLT S
Sbjct: 61  NKSEHIHRLMRILAHSGFFLIHKKTTQQEENDDADQE--VAYSLTNPSLLLLQHNPLTTS 120

Query: 128 PFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEGFNAAMES 187
           PF L +L++ L+ PW  +S W RS    RTPFET +GK+FWE+M SE +D E F   M S
Sbjct: 121 PFLLSVLNQSLLHPWHSLSTWFRSSGRDRTPFETTYGKKFWEYMESETRDREAFGEGMGS 180

Query: 188 DAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVAGLEG 247
           DA+LV+SVLL KYKS+FEGVESFVDVGGGTG++AKA+AEAFPQMKCVV+DLPQVVAGLEG
Sbjct: 181 DARLVISVLLGKYKSMFEGVESFVDVGGGTGTLAKAMAEAFPQMKCVVFDLPQVVAGLEG 240

Query: 248 NHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGK-NGKVIIID 307
           NHNL F QGDMFQ IPSA+ LLLKWILH+W+DDECV+ILKKCKEAI SN K   KVIIID
Sbjct: 241 NHNLTFRQGDMFQAIPSAEVLLLKWILHNWSDDECVQILKKCKEAIRSNNKLKEKVIIID 300

Query: 308 MVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFGAYNISP 367
           MV+ + S+       D + T+ +L WD+LMM +VGGKERDEKEWA+LF  AGFG+Y I P
Sbjct: 301 MVLPSSSSNNKA---DYISTQTQLLWDMLMMASVGGKERDEKEWAQLFHKAGFGSYKIFP 347

Query: 368 ILGLRSLIELYP 379
           ILGLRSLI+LYP
Sbjct: 361 ILGLRSLIKLYP 347

BLAST of CsaV3_7G025280 vs. ExPASy TrEMBL
Match: A0A6J1DVA5 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111023429 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 7.3e-132
Identity = 240/372 (64.52%), Postives = 293/372 (78.76%), Query Frame = 0

Query: 9   EMDGEEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHG-SPITLSQLISALQIHP 68
           E+ G EVL+A+ HIWNHIFSFINSMALKCAV+L IPD I  HG  P++LS L+SAL++HP
Sbjct: 3   EVAGNEVLEAETHIWNHIFSFINSMALKCAVQLGIPDTIAHHGRGPMSLSHLVSALKLHP 62

Query: 69  QKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQHNPLTLS 128
           + SQ++HR+MRILAHSGFF  H      K+D E E+    AYSLT+ S LLL+ NP +LS
Sbjct: 63  ENSQYLHRLMRILAHSGFFTAH------KNDKEQEE----AYSLTSSSRLLLKCNPSSLS 122

Query: 129 PFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEGFNAAMES 188
           P  L MLD  L++PW  +SAW  S    RT F+ AHGK+FWEHM S+ +DG+ FNAAM S
Sbjct: 123 PLLLSMLDPFLLQPWQLLSAWFHSHD--RTAFKMAHGKQFWEHMASKPRDGDVFNAAMAS 182

Query: 189 DAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVAGLEG 248
           DA+LV SVLL +YK++F+GV+S VDVGGGTG+VA+ IA+ FPQ++CVV DLP VVA LEG
Sbjct: 183 DARLVTSVLLGQYKNIFDGVKSLVDVGGGTGTVARVIADVFPQIQCVVLDLPHVVADLEG 242

Query: 249 NHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNGKVIIIDM 308
           +HNL +L+GDMFQ IPSAD LLLKWILHDW+D EC +ILKKCKEAITSNGK G+VI+IDM
Sbjct: 243 SHNLTYLEGDMFQSIPSADVLLLKWILHDWSDGECAEILKKCKEAITSNGKKGRVILIDM 302

Query: 309 VVGNKS-NKKNMKNNDMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGFGAYNISP 368
           VVGNK  +    ++ D +  + +LFWD+LMMVNVGGKERDEKEW +LF  AGFG Y I P
Sbjct: 303 VVGNKKVDGDGDRDGDDMFIETQLFWDMLMMVNVGGKERDEKEWVQLFDKAGFGGYKIFP 362

Query: 369 ILGLRSLIELYP 379
           ILGLRSLIE+YP
Sbjct: 363 ILGLRSLIEIYP 362

BLAST of CsaV3_7G025280 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 226.5 bits (576), Expect = 3.6e-59
Identity = 134/374 (35.83%), Postives = 199/374 (53.21%), Query Frame = 0

Query: 13  EEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGS--PITLSQLISALQIHPQKS 72
           +E  KA   IW ++F F +  A KCA++L+IP+AI  H S  P+TL++L SA+   P   
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASP--- 83

Query: 73  QHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNP----SLLLLQHNPLTL 132
            H+ RIMR L H G F             E   +D +A    N      L++ + +  +L
Sbjct: 84  SHLRRIMRFLVHQGIF------------KEIPTKDGLATGYVNTPLSRRLMITRRDGKSL 143

Query: 133 SPFSLFMLDRVLIEPWLFISAWLRSDSDCRT--PFETAHGKEFWEHMGSEAKDGEGFNAA 192
           +PF LF     ++ PWL +S+ + S  +  T  PF+  HGK+ W          +  N A
Sbjct: 144 APFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEA 203

Query: 193 MESDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVAG 252
           M  DA+ VV  +      +F+GV + VDVGGGTG     + + FP +K   +DLP V+  
Sbjct: 204 MACDARRVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEV 263

Query: 253 LEGNHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNGKVII 312
            E    +  ++GDMF  IP+ DA+ +KW+LHDW D +C+KILK CKEA+  N   GKV+I
Sbjct: 264 AEVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPN--IGKVLI 323

Query: 313 IDMVVGNKSNKKNMKNNDMLMTKGKLFWDLLMMVNVG-GKERDEKEWAELFQAAGFGAYN 372
           ++ V+G       +   D  +   +L  D++MM +   GKER  KEW  + + AGF  Y 
Sbjct: 324 VESVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYE 380

Query: 373 ISPILGLRSLIELY 378
           +  I  ++SLI  Y
Sbjct: 384 VRDIDDVQSLIIAY 380

BLAST of CsaV3_7G025280 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 199.1 bits (505), Expect = 6.2e-51
Identity = 127/368 (34.51%), Postives = 183/368 (49.73%), Query Frame = 0

Query: 13  EEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGS--PITLSQLISALQIHPQKS 72
           +E  KA   IW ++F F +  A KCA++L+IP+AI  H S  P+TLS+L SA+   P   
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASP--- 69

Query: 73  QHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQHNPLTLSPFS 132
            H+ RIMR L H G F             E   +D +A   TN             +P S
Sbjct: 70  SHLRRIMRFLVHQGLF------------KEVPTKDGLATGYTN-------------TPLS 129

Query: 133 LFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEGFNAAMESDAK 192
             M+   L                        HGK+ W          +  N AM  DA+
Sbjct: 130 RRMMITKL------------------------HGKDLWAFAQDNLCHSQLINEAMACDAR 189

Query: 193 LVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVAGLEGNHN 252
            VV  +    + +F+GV + VDVGGGTG     + + FP +K   +DLP V+   +    
Sbjct: 190 RVVPRVAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDG 249

Query: 253 LMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNGKVIIIDMVVG 312
           +  ++GDMF  IP++DA+++KW+LHDW D +C+KILK CKEA+  N   GKV+I++ V+G
Sbjct: 250 VENVEGDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPN--IGKVLIVECVIG 309

Query: 313 NKSNKKNMKNNDMLMTKGKLFWDLLMMVNVG-GKERDEKEWAELFQAAGFGAYNISPILG 372
            K N    +  D  +   +L  D++MMV+   GKER  KEW  +   AGF  Y +     
Sbjct: 310 EKKNTMIAEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDD 323

Query: 373 LRSLIELY 378
           ++SLI  Y
Sbjct: 370 VQSLIIAY 323

BLAST of CsaV3_7G025280 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 164.5 bits (415), Expect = 1.7e-40
Identity = 115/350 (32.86%), Postives = 188/350 (53.71%), Query Frame = 0

Query: 33  MALKCAVELRIPDAINRHGSPITLSQLISALQI-HPQKSQHVHRIMRIL-AHSGFFLIHN 92
           MALK A+EL + + + ++GSP++ +++ S L   +P+    + RI+R+L ++S     + 
Sbjct: 34  MALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNR 93

Query: 93  KLSQQKDDDEDEDEDQVAYSLTNPSLLLLQHNP--LTLSPFSLFMLDRVLIEPWLFISAW 152
           KLS         D  +  Y L  P    L  N   ++++   L   D+VL+E W  +   
Sbjct: 94  KLS--------GDGVERIYGL-GPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDA 153

Query: 153 LRSDSDCRTPFETAHGKEFWEHMGSEAKDGEGFNAAMESDAKLVVSVLLEKYKSVFEGVE 212
           +    D   PF  A+G   +E+ G++ +  + FN  M + + + +  +LE YK  FEG+ 
Sbjct: 154 I---LDGGIPFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLT 213

Query: 213 SFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVAGLEGNHNLMFLQGDMFQVIPSADAL 272
           S VDVGGG G+  K I   +P +K + +DLP V+     +  +  + GDMF  +P  DA+
Sbjct: 214 SLVDVGGGIGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAI 273

Query: 273 LLKWILHDWTDDECVKILKKCKEAITSNGKNGKVIIIDMVVGNKSNKKNMKNNDMLMTKG 332
            +KWI HDW+D+ CVK LK C E++    ++GKVI+ + ++    +         L TK 
Sbjct: 274 FMKWICHDWSDEHCVKFLKNCYESLP---EDGKVILAECILPETPDSS-------LSTKQ 333

Query: 333 KLFWDLLMMV-NVGGKERDEKEWAELFQAAGF-GAYNISPILGLRSLIEL 377
            +  D +M+  N GGKER EKE+  L +A+GF G   +    G+ +LIEL
Sbjct: 334 VVHVDCIMLAHNPGGKERTEKEFEALAKASGFKGIKVVCDAFGV-NLIEL 359

BLAST of CsaV3_7G025280 vs. TAIR 10
Match: AT1G63140.2 (O-methyltransferase family protein )

HSP 1 Score: 147.9 bits (372), Expect = 1.6e-35
Identity = 118/378 (31.22%), Postives = 185/378 (48.94%), Query Frame = 0

Query: 13  EEVLKAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLISALQI-----HP 72
           E+ +  QA    H  +F   M LK A+EL + D I      + LS    AL +     +P
Sbjct: 26  EDTVSLQAERILHAMTF--PMVLKTALELGVIDMITSVDDGVWLSPSEIALGLPTKPTNP 85

Query: 73  QKSQHVHRIMRILA-HSGFFLIHNKLSQQKDDDEDEDEDQVAYSLTNPSLLLLQHNPLTL 132
           +    + R++ +LA HS    I    + +  D+    + +  Y+        L       
Sbjct: 86  EAPVLLDRMLVLLASHS----ILKYRTVETGDNIGSRKTERVYAAEPVCTFFLNRGDGLG 145

Query: 133 SPFSLFML--DRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEHMGSEAKDGEGFNAA 192
           S  +LFM+    V ++PW  +   +    D    F +AHG  F+E +GS  +  E FN A
Sbjct: 146 SLATLFMVLQGEVCMKPWEHLKDMILEGKDA---FTSAHGMRFFELIGSNEQFAEMFNRA 205

Query: 193 MESDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQMKCVVYDLPQVVAG 252
           M   + L++  +LE YK  FE V + VDVGGG G++   +   +P +K + +DL  V+A 
Sbjct: 206 MSEASTLIMKKVLEVYKG-FEDVNTLVDVGGGIGTIIGQVTSKYPHIKGINFDLASVLAH 265

Query: 253 LEGNHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCKEAITSNGKNGKVII 312
              N  +  + GDMF+ IP  DA+ +KWILHDWTD++CVKILK   +++    + GKVII
Sbjct: 266 APFNKGVEHVSGDMFKEIPKGDAIFMKWILHDWTDEDCVKILKNYWKSLP---EKGKVII 325

Query: 313 IDMVVGNKSNKKNMKNN-----DMLMTKGKLFWDLLMMVNVGGKERDEKEWAELFQAAGF 372
           +++V   +    ++ +N     DMLM          + V+ GGKER   ++  L   +GF
Sbjct: 326 VEVVTPEEPKINDISSNIVFGMDMLM----------LAVSSGGKERSLSQFETLASDSGF 380

Query: 373 GAYNISPILGLRSLIELY 378
               I       S+IEL+
Sbjct: 386 LRCEIICHAFSYSVIELH 380

BLAST of CsaV3_7G025280 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 147.1 bits (370), Expect = 2.8e-35
Identity = 105/331 (31.72%), Postives = 172/331 (51.96%), Query Frame = 0

Query: 52  SPITLSQLISALQIHPQKSQHVHRIMRILAHSGFFLIHNKLSQQKDDDEDEDEDQVAYSL 111
           SP+ L+ +  A   +P     + R++R       FL+   +   K   ++E  +  AY L
Sbjct: 55  SPVDLASM--AAPKNPHAPMMIDRLLR-------FLVAYSVCTCKLVKDEEGRESRAYGL 114

Query: 112 TNPSLLLLQ-HNPLTLSPFSLFMLDRVLIEPWLFISAWLRSDSDCRTPFETAHGKEFWEH 171
                 L++  +  +++P+ L    +     W +++  ++      + +E A+    +E+
Sbjct: 115 GKVGKKLIKDEDGFSIAPYVLAGCTKAKGGVWSYLTEAIQEGG--ASAWERANEALIFEY 174

Query: 172 MGSEAKDGEGFNAAMESDAKLVVSVLLEKYKSVFEGVESFVDVGGGTGSVAKAIAEAFPQ 231
           M       + FN +M +   +V+  +LE Y   FEGV  FVDVGG  GS    I   +P 
Sbjct: 175 MKKNENLKKIFNESMTNHTSIVMKKILENYIG-FEGVSDFVDVGGSLGSNLAQILSKYPH 234

Query: 232 MKCVVYDLPQVVAGLEGNHNLMFLQGDMFQVIPSADALLLKWILHDWTDDECVKILKKCK 291
           +K + +DLP +V      H +  + GDMF  IP  + +L+KWILHDW D++CV+ILK CK
Sbjct: 235 IKGINFDLPHIVKEAPQIHGVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCK 294

Query: 292 EAITSNGKNGKVIIIDMVVGNKSNKKNMKNNDMLMTKGKLFWDLLMM-VNVGGKERDEKE 351
           +A+    + G++I+I+M+V  + ++ +      L TK  L  DL MM +  GGKER +KE
Sbjct: 295 KALP---ETGRIIVIEMIVPREVSETD------LATKNSLSADLTMMSLTSGGKERTKKE 354

Query: 352 WAELFQAAGFGAYNISPILGLRS--LIELYP 379
           + +L + AGF    I  I G  S  +IELYP
Sbjct: 355 FEDLAKEAGFKLPKI--IYGAYSYWIIELYP 362