CsaV3_7G022030 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_7G022030
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	Ribosomal RNA small subunit methyltransferase NEP1-like
Location	chr7: 10867977 .. 10869004 (-)
RNA-Seq Expression	CsaV3_7G022030
Synteny	CsaV3_7G022030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCCATACAAGTATAGACCTGATATAGTTCATGGGGTGAGTATAAAAAATTATCTTCAAAAGAGCAAAAGATGAAAATCCTCTAATATTGATACCACACTTTAGAATAGTAATGTTGAAAAAAAACCAACAGAATGGACTTGGTTTTTTATGAATTCTGAAATTCCATCTTTCCACCTAATCTAAAACTTTTCCTCAAAGTTTCACAATCTATTATTGAATCATGTTTTCCTTTGACCTTTAATAGAACTATCCTAAGCAAACACTTCGTAGTTTTGTGATGTTCTCTTTTGAACAAAATATAATCTTTAATTTTTCTTATGACAGGCTCTTGTCCATATCATGTATTCTCGAATTCGTATGGCTGGATTAGTTCAGGCAGTATTCATAAGAACTGATGAAGGAATTCTCATCAAAGTTGACCCTCGTACTCGAATACCAGAATCATTGGACGAGCTGTTTTGTGATATGGTGTGTTAGTTGTTCTTGCTTAGCAGAATACTAGTAGCTAGTTACGTGAATGAAACTAGTTCTTATGATGCTTCTATTTAAATTTCTTACATATGATTATTGTAGCTCAATTGTTGCAAAAGCTTAGCACTAAAGCCAAAGGCAACTGTGGAAAACTATTGCAAGTGGTAAAAAATCCTGTGATCCAATACTTACCTGTCAATTGCTTGAAGATCGGTGAGCTAATATATTTTGAAAGATTGATTAATTTATAGAAGTTTTTGTAACCTTGAATAATGACAATAATCCAGGTCTTTCCTCAAGTTCCAAAAAAGTTGTTGAACCACGAGATTACCTCAAAACGTTTAGCAACGACGTCAATCTAGTATTTGTGGTAATTACTCATCAGACACCGTAAAACTCTCCAATTTCTCATATTATGCATCAACTATCAAGCACTCTTTCCATTCTCATCATTTGGAGAGAAAACTAAATGATTATTATGTATTCAGATTGGAGCAATGGCTCATGGCAAAATTGACAATGAAAACATAGATGAATTGATATCAGGTTAG

mRNA sequence

ATGGATCCATACAAGTATAGACCTGATATAGTTCATGGGGCTCTTGTCCATATCATGTATTCTCGAATTCGTATGGCTGGATTAGTTCAGGCAGTATTCATAAGAACTGATGAAGGAATTCTCATCAAAGTTGACCCTCGTACTCGAATACCAGAATCATTGGACGAGCTGTTTTGTGATATGCTTAGCACTAAAGCCAAAGGCAACTGTGGAAAACTATTGCAAGTGGTAAAAAATCCTGTGATCCAATACTTACCTGTCAATTGCTTGAAGATCGGTCTTTCCTCAAGTTCCAAAAAAGTTGTTGAACCACGAGATTACCTCAAAACGTTTAGCAACGACGTCAATCTAGTATTTGTGATTGGAGCAATGGCTCATGGCAAAATTGACAATGAAAACATAGATGAATTGATATCAGGTTAG

Coding sequence (CDS)

ATGGATCCATACAAGTATAGACCTGATATAGTTCATGGGGCTCTTGTCCATATCATGTATTCTCGAATTCGTATGGCTGGATTAGTTCAGGCAGTATTCATAAGAACTGATGAAGGAATTCTCATCAAAGTTGACCCTCGTACTCGAATACCAGAATCATTGGACGAGCTGTTTTGTGATATGCTTAGCACTAAAGCCAAAGGCAACTGTGGAAAACTATTGCAAGTGGTAAAAAATCCTGTGATCCAATACTTACCTGTCAATTGCTTGAAGATCGGTCTTTCCTCAAGTTCCAAAAAAGTTGTTGAACCACGAGATTACCTCAAAACGTTTAGCAACGACGTCAATCTAGTATTTGTGATTGGAGCAATGGCTCATGGCAAAATTGACAATGAAAACATAGATGAATTGATATCAGGTTAG

Protein sequence

MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCDMLSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSNDVNLVFVIGAMAHGKIDNENIDELISG*

Homology

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BLAST of CsaV3_7G022030 vs. NCBI nr
Match: KGN44185.1 (hypothetical protein Csa_016257 [Cucumis sativus])

HSP 1 Score: 284.3 bits (726), Expect = 5.9e-73
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 1   MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD 60
           MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD
Sbjct: 1   MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD 60

Query: 61  MLSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSNDVNLVFV 120
           MLSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSNDVNLVFV
Sbjct: 61  MLSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSNDVNLVFV 120

Query: 121 IGAMAHGKIDNENIDELISG 141
           IGAMAHGKIDNENIDELISG
Sbjct: 121 IGAMAHGKIDNENIDELISG 140

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BLAST of CsaV3_7G022030 vs. NCBI nr
Match: XP_031745076.1 (ribosomal RNA small subunit methyltransferase NEP1-like [Cucumis sativus])

HSP 1 Score: 275.0 bits (702), Expect = 3.6e-70
Identity = 139/146 (95.21%), Postives = 139/146 (95.21%), Query Frame = 0


Query: 1   MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD 60
           MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD
Sbjct: 1   MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD 60

Query: 61  M-------LSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSN 120
           M       LSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSN
Sbjct: 61  MVSQLLQKLSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSN 120

Query: 121 DVNLVFVIGAMAHGKIDNENIDELIS 140
           DVNLVFVIGAMAHGKIDNENIDELIS
Sbjct: 121 DVNLVFVIGAMAHGKIDNENIDELIS 146

[Close]

BLAST of CsaV3_7G022030 vs. NCBI nr
Match: TYK19496.1 (ribosomal RNA small subunit methyltransferase NEP1-like [Cucumis melo var. makuwa])

HSP 1 Score: 253.4 bits (646), Expect = 1.1e-63
Identity = 129/146 (88.36%), Postives = 135/146 (92.47%), Query Frame = 0


Query: 1   MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD 60
           MDPYKYRPDIVH ALVHIMYSRI MAGLVQAVF+RTDEG+LIKVDPRTRIPESLDE FCD
Sbjct: 87  MDPYKYRPDIVHEALVHIMYSRICMAGLVQAVFVRTDEGVLIKVDPRTRIPESLDE-FCD 146

Query: 61  M-------LSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSN 120
           M       LSTKAKGN GKLLQVVKNPVIQYLPVNC+KIGLSSSSKKV+EPRDYLKTFSN
Sbjct: 147 MMSQLLQKLSTKAKGNRGKLLQVVKNPVIQYLPVNCVKIGLSSSSKKVIEPRDYLKTFSN 206

Query: 121 DVNLVFVIGAMAHGKIDNENIDELIS 140
           D+NLVFVIGAMAHGKID+ENIDELIS
Sbjct: 207 DINLVFVIGAMAHGKIDDENIDELIS 231

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BLAST of CsaV3_7G022030 vs. NCBI nr
Match: XP_008448880.1 (PREDICTED: ribosomal RNA small subunit methyltransferase NEP1-like [Cucumis melo])

HSP 1 Score: 251.9 bits (642), Expect = 3.2e-63
Identity = 129/146 (88.36%), Postives = 134/146 (91.78%), Query Frame = 0


Query: 1   MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD 60
           MDPYKYRPDIVH ALVHIMYSRI MAGLVQAVF+RTDEG+LIKVDP TRIPESLDE FCD
Sbjct: 40  MDPYKYRPDIVHEALVHIMYSRICMAGLVQAVFVRTDEGVLIKVDPHTRIPESLDE-FCD 99

Query: 61  M-------LSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSN 120
           M       LSTKAKGN GKLLQVVKNPVIQYLPVNC+KIGLSSSSKKV+EPRDYLKTFSN
Sbjct: 100 MMSQLLQKLSTKAKGNRGKLLQVVKNPVIQYLPVNCVKIGLSSSSKKVIEPRDYLKTFSN 159

Query: 121 DVNLVFVIGAMAHGKIDNENIDELIS 140
           DVNLVFVIGAMAHGKID+ENIDELIS
Sbjct: 160 DVNLVFVIGAMAHGKIDDENIDELIS 184

[Close]

BLAST of CsaV3_7G022030 vs. NCBI nr
Match: XP_022150056.1 (ribosomal RNA small subunit methyltransferase NEP1-like [Momordica charantia] >XP_022150057.1 ribosomal RNA small subunit methyltransferase NEP1-like [Momordica charantia])

HSP 1 Score: 218.8 bits (556), Expect = 3.0e-53
Identity = 110/146 (75.34%), Postives = 124/146 (84.93%), Query Frame = 0


Query: 1   MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD 60
           MDPY+YRPDI+H ALVHIM+SRI MAG+V+AVFI+TD GILI+VDPRTRIPESLDE FCD
Sbjct: 87  MDPYRYRPDILHEALVHIMHSRICMAGIVEAVFIKTDGGILIRVDPRTRIPESLDE-FCD 146

Query: 61  ML-------STKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSN 120
           M+       STKAKG  GKLLQ+VKNP+ QYLP+NC+KIGL+SSS KVVEPRDY  TFSN
Sbjct: 147 MMSQLLQKFSTKAKGKRGKLLQLVKNPITQYLPINCVKIGLTSSSTKVVEPRDYFTTFSN 206

Query: 121 DVNLVFVIGAMAHGKIDNENIDELIS 140
           DVN VFVIGAM H KID E IDE+IS
Sbjct: 207 DVNFVFVIGAMGHDKIDVEGIDEVIS 231

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BLAST of CsaV3_7G022030 vs. ExPASy Swiss-Prot
Match: O35130 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Mus musculus OX=10090 GN=Emg1 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 2.2e-22
Identity = 61/145 (42.07%), Postives = 93/145 (64.14%), Query Frame = 0


Query: 2   DPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCDM 61
           DP + RPDI H +L+ +M S +  AGL+Q V+I T + +LI+V+P+TRIP + D  FC +
Sbjct: 79  DPGEVRPDITHQSLLMLMDSPLNRAGLLQ-VYIHTQKNVLIEVNPQTRIPRTFDR-FCGL 138

Query: 62  -------LSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSND 121
                  LS +A     KLL+V+KNPV  + PV C+KIG S S + + + R+ +   S+D
Sbjct: 139 MVQLLHKLSVRAADGPQKLLKVIKNPVSDHFPVGCMKIGTSFSVEDISDIRELVP--SSD 198

Query: 122 VNLVFVIGAMAHGKIDNENIDELIS 140
             +VFV+GA AHGK+  E  ++++S
Sbjct: 199 -PVVFVVGAFAHGKVSVEYTEKMVS 218

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BLAST of CsaV3_7G022030 vs. ExPASy Swiss-Prot
Match: Q92979 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Homo sapiens OX=9606 GN=EMG1 PE=1 SV=4)

HSP 1 Score: 105.9 bits (263), Expect = 3.8e-22
Identity = 61/145 (42.07%), Postives = 92/145 (63.45%), Query Frame = 0


Query: 2   DPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCDM 61
           DP + RPDI H +L+ +M S +  AGL+Q V+I T + +LI+V+P+TRIP + D  FC +
Sbjct: 79  DPGEARPDITHQSLLMLMDSPLNRAGLLQ-VYIHTQKNVLIEVNPQTRIPRTFDR-FCGL 138

Query: 62  -------LSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSND 121
                  LS +A     KLL+V+KNPV  + PV C+K+G S S   V + R+ +   S+D
Sbjct: 139 MVQLLHKLSVRAADGPQKLLKVIKNPVSDHFPVGCMKVGTSFSIPVVSDVRELVP--SSD 198

Query: 122 VNLVFVIGAMAHGKIDNENIDELIS 140
             +VFV+GA AHGK+  E  ++++S
Sbjct: 199 -PIVFVVGAFAHGKVSVEYTEKMVS 218

[Close]

BLAST of CsaV3_7G022030 vs. ExPASy Swiss-Prot
Match: Q9W4J5 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Drosophila melanogaster OX=7227 GN=CG3527 PE=3 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 5.6e-18
Identity = 54/149 (36.24%), Postives = 83/149 (55.70%), Query Frame = 0


Query: 2   DPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDE---LF 61
           DP   RPDI H  L+ +  S +  AGL+Q VF+RT+  +LI+++P+TRIP +      L 
Sbjct: 79  DPGSCRPDITHQCLLMLFDSPLNRAGLLQ-VFVRTEHNVLIEINPQTRIPRTFKRFAGLM 138

Query: 62  CDML---STKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYL-----KT 121
             +L     +A  +  +L+ V+KNP+  ++PV C K  +S S K +   RD +      +
Sbjct: 139 VQLLHKFQIRANDSSRRLMSVIKNPITDHVPVGCKKYAMSFSGKLLPNCRDLVPHGDETS 198

Query: 122 FSNDVNLVFVIGAMAHGKIDNENIDELIS 140
            S D  +V VIGA AHG +  +  +EL S
Sbjct: 199 ASYDEPVVIVIGAFAHGVLKTDYTEELFS 226

[Close]

BLAST of CsaV3_7G022030 vs. ExPASy Swiss-Prot
Match: Q10107 (Ribosomal RNA small subunit methyltransferase mra1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mra1 PE=1 SV=2)

HSP 1 Score: 89.0 bits (219), Expect = 4.8e-17
Identity = 56/141 (39.72%), Postives = 78/141 (55.32%), Query Frame = 0


Query: 7   RPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDEL------FCD 66
           RPDI H  L+ ++ S +  AG +Q V+I T + +LI+V+P  RIP +             
Sbjct: 195 RPDITHQCLLTLLDSPLNKAGRLQ-VYIHTAKKVLIEVNPSVRIPRTFKRFSGLMVQLLH 254

Query: 67  MLSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSNDVNLVFV 126
            LS ++     KLL+V+KNPV  YLP NC K  LS  +   V PR YL+T   + ++   
Sbjct: 255 KLSIRSVNGNEKLLKVIKNPVTDYLPPNCRKATLSFDA-PTVPPRKYLETLQPNQSVCIA 314

Query: 127 IGAMAHGKIDNEN--IDELIS 140
           IGAMAHG  D  +  +DE IS
Sbjct: 315 IGAMAHGPDDFSDGWVDEKIS 333

[Close]

BLAST of CsaV3_7G022030 vs. ExPASy Swiss-Prot
Match: Q06287 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=EMG1 PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.4e-16
Identity = 51/140 (36.43%), Postives = 80/140 (57.14%), Query Frame = 0


Query: 7   RPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDEL------FCD 66
           RPDI H  L+ ++ S I  AG +Q V+I+T  GILI+V+P  RIP +             
Sbjct: 88  RPDITHQCLLTLLDSPINKAGKLQ-VYIQTSRGILIEVNPTVRIPRTFKRFSGLMVQLLH 147

Query: 67  MLSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSNDVNLVFV 126
            LS ++  +  KLL+V+KNP+  +LP  C K+ LS  +  V+  +DY++   +D ++   
Sbjct: 148 KLSIRSVNSEEKLLKVIKNPITDHLPTKCRKVTLSFDA-PVIRVQDYIEKLDDDESICVF 207

Query: 127 IGAMAHGK--IDNENIDELI 139
           +GAMA GK    +E +DE +
Sbjct: 208 VGAMARGKDNFADEYVDEKV 225

[Close]

BLAST of CsaV3_7G022030 vs. ExPASy TrEMBL
Match: A0A0A0K3C1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G219210 PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 2.8e-73
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 1   MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD 60
           MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD
Sbjct: 1   MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD 60

Query: 61  MLSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSNDVNLVFV 120
           MLSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSNDVNLVFV
Sbjct: 61  MLSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSNDVNLVFV 120

Query: 121 IGAMAHGKIDNENIDELISG 141
           IGAMAHGKIDNENIDELISG
Sbjct: 121 IGAMAHGKIDNENIDELISG 140

[Close]

BLAST of CsaV3_7G022030 vs. ExPASy TrEMBL
Match: A0A5D3D7D3 (Ribosomal RNA small subunit methyltransferase NEP1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G001510 PE=3 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 5.4e-64
Identity = 129/146 (88.36%), Postives = 135/146 (92.47%), Query Frame = 0


Query: 1   MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD 60
           MDPYKYRPDIVH ALVHIMYSRI MAGLVQAVF+RTDEG+LIKVDPRTRIPESLDE FCD
Sbjct: 87  MDPYKYRPDIVHEALVHIMYSRICMAGLVQAVFVRTDEGVLIKVDPRTRIPESLDE-FCD 146

Query: 61  M-------LSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSN 120
           M       LSTKAKGN GKLLQVVKNPVIQYLPVNC+KIGLSSSSKKV+EPRDYLKTFSN
Sbjct: 147 MMSQLLQKLSTKAKGNRGKLLQVVKNPVIQYLPVNCVKIGLSSSSKKVIEPRDYLKTFSN 206

Query: 121 DVNLVFVIGAMAHGKIDNENIDELIS 140
           D+NLVFVIGAMAHGKID+ENIDELIS
Sbjct: 207 DINLVFVIGAMAHGKIDDENIDELIS 231

[Close]

BLAST of CsaV3_7G022030 vs. ExPASy TrEMBL
Match: A0A1S3BLD0 (ribosomal RNA small subunit methyltransferase NEP1-like OS=Cucumis melo OX=3656 GN=LOC103490911 PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.6e-63
Identity = 129/146 (88.36%), Postives = 134/146 (91.78%), Query Frame = 0


Query: 1   MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD 60
           MDPYKYRPDIVH ALVHIMYSRI MAGLVQAVF+RTDEG+LIKVDP TRIPESLDE FCD
Sbjct: 40  MDPYKYRPDIVHEALVHIMYSRICMAGLVQAVFVRTDEGVLIKVDPHTRIPESLDE-FCD 99

Query: 61  M-------LSTKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSN 120
           M       LSTKAKGN GKLLQVVKNPVIQYLPVNC+KIGLSSSSKKV+EPRDYLKTFSN
Sbjct: 100 MMSQLLQKLSTKAKGNRGKLLQVVKNPVIQYLPVNCVKIGLSSSSKKVIEPRDYLKTFSN 159

Query: 121 DVNLVFVIGAMAHGKIDNENIDELIS 140
           DVNLVFVIGAMAHGKID+ENIDELIS
Sbjct: 160 DVNLVFVIGAMAHGKIDDENIDELIS 184

[Close]

BLAST of CsaV3_7G022030 vs. ExPASy TrEMBL
Match: A0A6J1DAB0 (ribosomal RNA small subunit methyltransferase NEP1-like OS=Momordica charantia OX=3673 GN=LOC111018323 PE=3 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 1.5e-53
Identity = 110/146 (75.34%), Postives = 124/146 (84.93%), Query Frame = 0


Query: 1   MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD 60
           MDPY+YRPDI+H ALVHIM+SRI MAG+V+AVFI+TD GILI+VDPRTRIPESLDE FCD
Sbjct: 87  MDPYRYRPDILHEALVHIMHSRICMAGIVEAVFIKTDGGILIRVDPRTRIPESLDE-FCD 146

Query: 61  ML-------STKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSN 120
           M+       STKAKG  GKLLQ+VKNP+ QYLP+NC+KIGL+SSS KVVEPRDY  TFSN
Sbjct: 147 MMSQLLQKFSTKAKGKRGKLLQLVKNPITQYLPINCVKIGLTSSSTKVVEPRDYFTTFSN 206

Query: 121 DVNLVFVIGAMAHGKIDNENIDELIS 140
           DVN VFVIGAM H KID E IDE+IS
Sbjct: 207 DVNFVFVIGAMGHDKIDVEGIDEVIS 231

[Close]

BLAST of CsaV3_7G022030 vs. ExPASy TrEMBL
Match: M5XH03 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa012263mg PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 9.2e-40
Identity = 89/143 (62.24%), Postives = 110/143 (76.92%), Query Frame = 0


Query: 1   MDPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDELFCD 60
           MDPYKYRPDIVH AL  IM +R+  AG + AV+IRTDEG+LIKV+PR  IPESL E FC 
Sbjct: 10  MDPYKYRPDIVHEALRRIMDTRLCKAGRLHAVYIRTDEGVLIKVEPRATIPESL-EKFCS 69

Query: 61  ML----STKAKGNCGKLLQVVKNPVIQYLPVNCLKIGLSSSSKKVVEPRDYLKTFSNDVN 120
            L    S K+ G  G LL+VVKNPV Q+LP N LKIGLS SS+K V+ RDY+   S++ N
Sbjct: 70  QLLQKFSIKSTGGRGSLLRVVKNPVAQHLPANSLKIGLSLSSQKAVDLRDYVHDVSDNAN 129

Query: 121 LVFVIGAMAHGKIDNENIDELIS 140
           LVFV+GAMAHG+I++EN+D++IS
Sbjct: 130 LVFVVGAMAHGRIESENVDDIIS 151

[Close]

BLAST of CsaV3_7G022030 vs. TAIR 10
Match: AT3G57000.1 (nucleolar essential protein-related )

HSP 1 Score: 113.2 bits (282), Expect = 1.7e-25
Identity = 64/146 (43.84%), Postives = 91/146 (62.33%), Query Frame = 0


Query: 2   DPYKYRPDIVHGALVHIMYSRIRMAGLVQAVFIRTDEGILIKVDPRTRIPESLDEL---- 61
           +P  YRPDI H AL+ I+ S +  AG ++AV++RT++G+L +V P  RIP +        
Sbjct: 127 NPADYRPDITHQALLMILDSPVNKAGRLKAVYVRTEKGVLFEVKPHVRIPRTFKRFAGIM 186

Query: 62  --FCDMLSTKAKGNCGKLLQVVKNPVIQ-YLPVNCLKIGLSSSSKKVVEPRDYLKTF-SN 121
                 LS  A  +  KLL+ VKNP+ + +LPVN  +IG S SS+K+V  + +L T   +
Sbjct: 187 LQLLQKLSITAVNSREKLLRCVKNPIEEHHLPVNSHRIGFSHSSEKLVNMQKHLATVCDD 246

Query: 122 DVNLVFVIGAMAHGKIDNENIDELIS 140
           D + VFV+GAMAHGKID   IDE +S
Sbjct: 247 DRDTVFVVGAMAHGKIDCNYIDEFVS 272

The following BLAST results are available for this feature:

BLAST of CsaV3_7G022030 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN44185.1	5.9e-73	100.00	hypothetical protein Csa_016257 [Cucumis sativus]	[more]
XP_031745076.1	3.6e-70	95.21	ribosomal RNA small subunit methyltransferase NEP1-like [Cucumis sativus]	[more]
TYK19496.1	1.1e-63	88.36	ribosomal RNA small subunit methyltransferase NEP1-like [Cucumis melo var. makuw...	[more]
XP_008448880.1	3.2e-63	88.36	PREDICTED: ribosomal RNA small subunit methyltransferase NEP1-like [Cucumis melo...	[more]
XP_022150056.1	3.0e-53	75.34	ribosomal RNA small subunit methyltransferase NEP1-like [Momordica charantia] >X...	[more]

BLAST of CsaV3_7G022030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O35130	2.2e-22	42.07	Ribosomal RNA small subunit methyltransferase NEP1 OS=Mus musculus OX=10090 GN=E...	[more]
Q92979	3.8e-22	42.07	Ribosomal RNA small subunit methyltransferase NEP1 OS=Homo sapiens OX=9606 GN=EM...	[more]
Q9W4J5	5.6e-18	36.24	Ribosomal RNA small subunit methyltransferase NEP1 OS=Drosophila melanogaster OX...	[more]
Q10107	4.8e-17	39.72	Ribosomal RNA small subunit methyltransferase mra1 OS=Schizosaccharomyces pombe ...	[more]
Q06287	1.4e-16	36.43	Ribosomal RNA small subunit methyltransferase NEP1 OS=Saccharomyces cerevisiae (...	[more]

BLAST of CsaV3_7G022030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K3C1	2.8e-73	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G219210 PE=3 SV=1	[more]
A0A5D3D7D3	5.4e-64	88.36	Ribosomal RNA small subunit methyltransferase NEP1-like OS=Cucumis melo var. mak...	[more]
A0A1S3BLD0	1.6e-63	88.36	ribosomal RNA small subunit methyltransferase NEP1-like OS=Cucumis melo OX=3656 ...	[more]
A0A6J1DAB0	1.5e-53	75.34	ribosomal RNA small subunit methyltransferase NEP1-like OS=Momordica charantia O...	[more]
M5XH03	9.2e-40	62.24	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa012263mg PE=3 SV=1	[more]

BLAST of CsaV3_7G022030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G57000.1	1.7e-25	43.84	nucleolar essential protein-related	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005304	Ribosomal biogenesis, methyltransferase, EMG1/NEP1	PFAM	PF03587	EMG1	coord: 3..135 e-value: 4.3E-27 score: 95.1
IPR005304	Ribosomal biogenesis, methyltransferase, EMG1/NEP1	PANTHER	PTHR12636	NEP1/MRA1	coord: 1..139
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 1..140 e-value: 1.6E-36 score: 127.7
None	No IPR available	PANTHER	PTHR12636:SF12	EMG1/NEP1 METHYLTRANSFERASE	coord: 1..139
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 3..137

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_7G022030.1	CsaV3_7G022030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070475	rRNA base methylation
molecular_function	GO:0070037	rRNA (pseudouridine) methyltransferase activity