CsaV3_6G051790 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_6G051790
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description9-cis-epoxycarotenoid dioxygenase, chloroplastic
Locationchr6: 30131435 .. 30133535 (+)
RNA-Seq ExpressionCsaV3_6G051790
SyntenyCsaV3_6G051790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGCGCCATGACGGCTTCTCCTTCTCCCTTACTCGATCATTTGGGATTCCTAAATTCCACCAAATCAAATTTCCCGAATTTCAATATCAATAATCTTAAACCCATTACTTCCCAAAACTCCATTATTCACTCTGCTCTTCATTCCCCTTCCGTTCTTCATTTCCCAAATCAAACTACTACTTCTACTACTACTACTTCTACTACTCCTAATTTCCATAAATCCTTGTCTCTCAAACATCAAAAATCCCCCACTGCTCCCAATTGGAATCTCCTTCAAAAAACTGCTTCCATGGCTTTAGATATGGTCGAATCCGCTTTAGTATCTCACGAGCTTCAACACCCACTTCCTAAAACCGCCGACCCACGTGTTCAAATCTCCGGCAACTTTGCTCCCGTCCCGGAACAAGCCGTTAAACACTACCTGCCCGTTGCCGGAACTATCCCCGATTGCATTAACGGGGTTTACCTTCGCAACGGCGCCAATCCTCTCTTCCAACCAACTGCCGGGCACCATCTCTTCGACGGCGATGGAATGGTTCACGCTGTCAGCATCAATCATGGTTCTGCTAGTTACGCTTGCCGGTTCACTCAAACCCAACGCTTAGTTCAGGAACGGCGATTGGGGAGACCGGTTTTTCCTAAAGCCATTGGGGAACTCCATGGCCACTCCGGAATCGCTCGCCTCTTACTATTCTACGCAAGAGGAGTTTTCGGGTTGGTGGACCATAAGAAAGGAACCGGAGTGGCGAACGCCGGTTTGGTGTATTTCAATGACCGGCTTTTGGCGATGTCAGAAGACGATTTGCCGTACCACGTGCGTATCACGCCTTCAGGTGATCTACAAACCGTAGGCCGTTACGATTTCGATAAACAGCTTCATTCCACAATGATCGCTCATCCCAAAGTGGACCCAATTTCCAAAGAACTCTACGCCTTGAGCTACGACGTCGTTCGTAAACCGTATCTCAAATACTTCAGATTCTCCCCTAATGGAATCAAATCAAAAGACGTTGAAATCCCACTTGAAACTCCCACAATGATGCACGATTTCGCCATCACAGAGAATTTCGTTGTGATTCCCGACCAGCAAGTGGTGTTCAAGCTTCAAGAAATGGTGAAAGGCGGATCGCCGGTGATATACGACAAGAACAAGAAATCCCGATTCGGGATTCTGCCGAAAAATGCGACGGATTCAAAGGATTTGATTTGGGTGGAATCCCCGGACACGTTCTGCTTCCATCTATGGAATGCTTGGGAGGAACCGGAGACGGAGGAAGTGGTGGTGATTGGGTCGTGTATGACGCCCCCGGATTCGATATTCAACGAGTGTGAGGAGAATCTGAAAAGCGTATTATCGGAAATCCGGCTGAATTTACGGACGGGGAAATCGACACGCCGGGCAATAATTAAGGAAGAGAAAGAACAGGTGAATCTAGAAGCGGGAATGGTGAATAAAAACCGTGTGGGAAGGAAAAGCCGGTATGCGTATTTGGCGATTGCAGAGCCATGGCCAAAGGTATCGGGATTTGCGAAAGTTGATCTGGTAACAGGGGAAGTAAAAAAACATATTTACGGAGGTAGAAAATTCGGTGGGGAACCATTTTTTTTACCGAAAGAGGAAAATTCAAAGGAAGAAGATGAAGGGTATATTCTGGCGTTTGTTCATGATGAAAGGACGTGGAAATCAGAGGTACAGATAGTAAACGCAAAGGATTTGAAAGTGGAAGCTACAATAAAATTACCATCTCGAGTACCTTATGGATTTCACGGCACATTCGTTCAATCGAATGATTTACAAAATCAAGCTTAATTCATGATTGGAGGATCAAAAGAAAAAAAAAAAAGAAGGAGAAATTACCAGAGGGATGACTTAGAGACAGGTCCCCGGATTCTCTCCTGTTAGCATTAAAAACTGAACAACTTTTTTTGTTTGTGTTTTCTTCTTTTGAATTTTAAATTTTTAATTTTGAGTGAGTGATAGATAATTTGAAGACGAAACTCAGCTTGTAGCTTTTGGACAGTTTCGTAGATTTCGAGCTCAGCTGGTTTCTGTTTCTTTGTTTTCTTTTTCTTTTTCTTTTTCTTTTTCCTCTCTCTGT

mRNA sequence

ATGACGGCTTCTCCTTCTCCCTTACTCGATCATTTGGGATTCCTAAATTCCACCAAATCAAATTTCCCGAATTTCAATATCAATAATCTTAAACCCATTACTTCCCAAAACTCCATTATTCACTCTGCTCTTCATTCCCCTTCCGTTCTTCATTTCCCAAATCAAACTACTACTTCTACTACTACTACTTCTACTACTCCTAATTTCCATAAATCCTTGTCTCTCAAACATCAAAAATCCCCCACTGCTCCCAATTGGAATCTCCTTCAAAAAACTGCTTCCATGGCTTTAGATATGGTCGAATCCGCTTTAGTATCTCACGAGCTTCAACACCCACTTCCTAAAACCGCCGACCCACGTGTTCAAATCTCCGGCAACTTTGCTCCCGTCCCGGAACAAGCCGTTAAACACTACCTGCCCGTTGCCGGAACTATCCCCGATTGCATTAACGGGGTTTACCTTCGCAACGGCGCCAATCCTCTCTTCCAACCAACTGCCGGGCACCATCTCTTCGACGGCGATGGAATGGTTCACGCTGTCAGCATCAATCATGGTTCTGCTAGTTACGCTTGCCGGTTCACTCAAACCCAACGCTTAGTTCAGGAACGGCGATTGGGGAGACCGGTTTTTCCTAAAGCCATTGGGGAACTCCATGGCCACTCCGGAATCGCTCGCCTCTTACTATTCTACGCAAGAGGAGTTTTCGGGTTGGTGGACCATAAGAAAGGAACCGGAGTGGCGAACGCCGGTTTGGTGTATTTCAATGACCGGCTTTTGGCGATGTCAGAAGACGATTTGCCGTACCACGTGCGTATCACGCCTTCAGGTGATCTACAAACCGTAGGCCGTTACGATTTCGATAAACAGCTTCATTCCACAATGATCGCTCATCCCAAAGTGGACCCAATTTCCAAAGAACTCTACGCCTTGAGCTACGACGTCGTTCGTAAACCGTATCTCAAATACTTCAGATTCTCCCCTAATGGAATCAAATCAAAAGACGTTGAAATCCCACTTGAAACTCCCACAATGATGCACGATTTCGCCATCACAGAGAATTTCGTTGTGATTCCCGACCAGCAAGTGGTGTTCAAGCTTCAAGAAATGGTGAAAGGCGGATCGCCGGTGATATACGACAAGAACAAGAAATCCCGATTCGGGATTCTGCCGAAAAATGCGACGGATTCAAAGGATTTGATTTGGGTGGAATCCCCGGACACGTTCTGCTTCCATCTATGGAATGCTTGGGAGGAACCGGAGACGGAGGAAGTGGTGGTGATTGGGTCGTGTATGACGCCCCCGGATTCGATATTCAACGAGTGTGAGGAGAATCTGAAAAGCGTATTATCGGAAATCCGGCTGAATTTACGGACGGGGAAATCGACACGCCGGGCAATAATTAAGGAAGAGAAAGAACAGGTGAATCTAGAAGCGGGAATGGTGAATAAAAACCGTGTGGGAAGGAAAAGCCGGTATGCGTATTTGGCGATTGCAGAGCCATGGCCAAAGGTATCGGGATTTGCGAAAGTTGATCTGGTAACAGGGGAAGTAAAAAAACATATTTACGGAGGTAGAAAATTCGGTGGGGAACCATTTTTTTTACCGAAAGAGGAAAATTCAAAGGAAGAAGATGAAGGGTATATTCTGGCGTTTGTTCATGATGAAAGGACGTGGAAATCAGAGGTACAGATAGTAAACGCAAAGGATTTGAAAGTGGAAGCTACAATAAAATTACCATCTCGAGTACCTTATGGATTTCACGGCACATTCGTTCAATCGAATGATTTACAAAATCAAGCTTAA

Coding sequence (CDS)

ATGACGGCTTCTCCTTCTCCCTTACTCGATCATTTGGGATTCCTAAATTCCACCAAATCAAATTTCCCGAATTTCAATATCAATAATCTTAAACCCATTACTTCCCAAAACTCCATTATTCACTCTGCTCTTCATTCCCCTTCCGTTCTTCATTTCCCAAATCAAACTACTACTTCTACTACTACTACTTCTACTACTCCTAATTTCCATAAATCCTTGTCTCTCAAACATCAAAAATCCCCCACTGCTCCCAATTGGAATCTCCTTCAAAAAACTGCTTCCATGGCTTTAGATATGGTCGAATCCGCTTTAGTATCTCACGAGCTTCAACACCCACTTCCTAAAACCGCCGACCCACGTGTTCAAATCTCCGGCAACTTTGCTCCCGTCCCGGAACAAGCCGTTAAACACTACCTGCCCGTTGCCGGAACTATCCCCGATTGCATTAACGGGGTTTACCTTCGCAACGGCGCCAATCCTCTCTTCCAACCAACTGCCGGGCACCATCTCTTCGACGGCGATGGAATGGTTCACGCTGTCAGCATCAATCATGGTTCTGCTAGTTACGCTTGCCGGTTCACTCAAACCCAACGCTTAGTTCAGGAACGGCGATTGGGGAGACCGGTTTTTCCTAAAGCCATTGGGGAACTCCATGGCCACTCCGGAATCGCTCGCCTCTTACTATTCTACGCAAGAGGAGTTTTCGGGTTGGTGGACCATAAGAAAGGAACCGGAGTGGCGAACGCCGGTTTGGTGTATTTCAATGACCGGCTTTTGGCGATGTCAGAAGACGATTTGCCGTACCACGTGCGTATCACGCCTTCAGGTGATCTACAAACCGTAGGCCGTTACGATTTCGATAAACAGCTTCATTCCACAATGATCGCTCATCCCAAAGTGGACCCAATTTCCAAAGAACTCTACGCCTTGAGCTACGACGTCGTTCGTAAACCGTATCTCAAATACTTCAGATTCTCCCCTAATGGAATCAAATCAAAAGACGTTGAAATCCCACTTGAAACTCCCACAATGATGCACGATTTCGCCATCACAGAGAATTTCGTTGTGATTCCCGACCAGCAAGTGGTGTTCAAGCTTCAAGAAATGGTGAAAGGCGGATCGCCGGTGATATACGACAAGAACAAGAAATCCCGATTCGGGATTCTGCCGAAAAATGCGACGGATTCAAAGGATTTGATTTGGGTGGAATCCCCGGACACGTTCTGCTTCCATCTATGGAATGCTTGGGAGGAACCGGAGACGGAGGAAGTGGTGGTGATTGGGTCGTGTATGACGCCCCCGGATTCGATATTCAACGAGTGTGAGGAGAATCTGAAAAGCGTATTATCGGAAATCCGGCTGAATTTACGGACGGGGAAATCGACACGCCGGGCAATAATTAAGGAAGAGAAAGAACAGGTGAATCTAGAAGCGGGAATGGTGAATAAAAACCGTGTGGGAAGGAAAAGCCGGTATGCGTATTTGGCGATTGCAGAGCCATGGCCAAAGGTATCGGGATTTGCGAAAGTTGATCTGGTAACAGGGGAAGTAAAAAAACATATTTACGGAGGTAGAAAATTCGGTGGGGAACCATTTTTTTTACCGAAAGAGGAAAATTCAAAGGAAGAAGATGAAGGGTATATTCTGGCGTTTGTTCATGATGAAAGGACGTGGAAATCAGAGGTACAGATAGTAAACGCAAAGGATTTGAAAGTGGAAGCTACAATAAAATTACCATCTCGAGTACCTTATGGATTTCACGGCACATTCGTTCAATCGAATGATTTACAAAATCAAGCTTAA

Protein sequence

MTASPSPLLDHLGFLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQTTTSTTTTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPRVQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAVSINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENSKEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQNQA*
Homology
BLAST of CsaV3_6G051790 vs. NCBI nr
Match: XP_004134911.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic [Cucumis sativus] >KGN49403.1 hypothetical protein Csa_004240 [Cucumis sativus])

HSP 1 Score: 1223.4 bits (3164), Expect = 0.0e+00
Identity = 600/600 (100.00%), Postives = 600/600 (100.00%), Query Frame = 0

Query: 1   MTASPSPLLDHLGFLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQTTTST 60
           MTASPSPLLDHLGFLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQTTTST
Sbjct: 1   MTASPSPLLDHLGFLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQTTTST 60

Query: 61  TTTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPR 120
           TTTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPR
Sbjct: 61  TTTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPR 120

Query: 121 VQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV 180
           VQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV
Sbjct: 121 VQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV 180

Query: 181 SINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDH 240
           SINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDH
Sbjct: 181 SINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDH 240

Query: 241 KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV 300
           KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV
Sbjct: 241 KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV 300

Query: 301 DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQ 360
           DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQ
Sbjct: 301 DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQ 360

Query: 361 QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE 420
           QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE
Sbjct: 361 QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE 420

Query: 421 TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM 480
           TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM
Sbjct: 421 TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM 480

Query: 481 VNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENS 540
           VNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENS
Sbjct: 481 VNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENS 540

Query: 541 KEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQNQA 600
           KEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQNQA
Sbjct: 541 KEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQNQA 600

BLAST of CsaV3_6G051790 vs. NCBI nr
Match: XP_008439625.1 (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1169.5 bits (3024), Expect = 0.0e+00
Identity = 577/603 (95.69%), Postives = 585/603 (97.01%), Query Frame = 0

Query: 1   MTASPSPLLDHLGFLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQTTTST 60
           MTA PSPLLDHLGFLNSTKSNFPN NIN+LK IT+QNSIIHSALHSPSVL FPNQ    T
Sbjct: 1   MTAPPSPLLDHLGFLNSTKSNFPNLNINHLKSITTQNSIIHSALHSPSVLRFPNQ----T 60

Query: 61  TTTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPR 120
           TTT   PNFHKSLSLKHQKSPTAPNWNLLQKTAS+ALDMVESALVSHELQHPLPKTADPR
Sbjct: 61  TTTPPPPNFHKSLSLKHQKSPTAPNWNLLQKTASIALDMVESALVSHELQHPLPKTADPR 120

Query: 121 VQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV 180
           VQISGNFAPVPEQ VKH LPV GTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV
Sbjct: 121 VQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV 180

Query: 181 SINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDH 240
           +INHGSASYACRFTQTQRLVQERRLGRP+FPKAIGELHGHSGIARLLLFYARGVFGLVDH
Sbjct: 181 TINHGSASYACRFTQTQRLVQERRLGRPIFPKAIGELHGHSGIARLLLFYARGVFGLVDH 240

Query: 241 KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV 300
           KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV
Sbjct: 241 KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV 300

Query: 301 DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQ 360
           DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIP+ETPTMMHDFAITENFVVIPDQ
Sbjct: 301 DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPVETPTMMHDFAITENFVVIPDQ 360

Query: 361 QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE 420
           QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE
Sbjct: 361 QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE 420

Query: 421 TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM 480
           TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM
Sbjct: 421 TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM 480

Query: 481 VNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENS 540
           VNK RVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPK+ENS
Sbjct: 481 VNKTRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKDENS 540

Query: 541 K---EEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQ 600
           K   EEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSN+LQ
Sbjct: 541 KAEEEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNNLQ 599

BLAST of CsaV3_6G051790 vs. NCBI nr
Match: KAA0052537.1 (9-cis-epoxycarotenoid dioxygenase NCED3 [Cucumis melo var. makuwa])

HSP 1 Score: 1166.4 bits (3016), Expect = 0.0e+00
Identity = 576/603 (95.52%), Postives = 584/603 (96.85%), Query Frame = 0

Query: 1   MTASPSPLLDHLGFLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQTTTST 60
           MTA PS LLDHLGFLNSTKSNFPN NIN+LK IT+QNSIIHSALHSPSVL FPNQ    T
Sbjct: 1   MTAPPSALLDHLGFLNSTKSNFPNLNINHLKSITTQNSIIHSALHSPSVLRFPNQ----T 60

Query: 61  TTTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPR 120
           TTT   PNFHKSLSLKHQKSPTAPNWNLLQKTAS+ALDMVESALVSHELQHPLPKTADPR
Sbjct: 61  TTTPPPPNFHKSLSLKHQKSPTAPNWNLLQKTASIALDMVESALVSHELQHPLPKTADPR 120

Query: 121 VQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV 180
           VQISGNFAPVPEQ VKH LPV GTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV
Sbjct: 121 VQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV 180

Query: 181 SINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDH 240
           +INHGSASYACRFTQTQRLVQERRLGRP+FPKAIGELHGHSGIARLLLFYARGVFGLVDH
Sbjct: 181 TINHGSASYACRFTQTQRLVQERRLGRPIFPKAIGELHGHSGIARLLLFYARGVFGLVDH 240

Query: 241 KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV 300
           KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV
Sbjct: 241 KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV 300

Query: 301 DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQ 360
           DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIP+ETPTMMHDFAITENFVVIPDQ
Sbjct: 301 DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPVETPTMMHDFAITENFVVIPDQ 360

Query: 361 QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE 420
           QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE
Sbjct: 361 QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE 420

Query: 421 TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM 480
           TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM
Sbjct: 421 TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM 480

Query: 481 VNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENS 540
           VNK RVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPK+ENS
Sbjct: 481 VNKTRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKDENS 540

Query: 541 K---EEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQ 600
           K   EEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSN+LQ
Sbjct: 541 KAEEEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNNLQ 599

BLAST of CsaV3_6G051790 vs. NCBI nr
Match: XP_038883946.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1098.6 bits (2840), Expect = 0.0e+00
Identity = 551/610 (90.33%), Postives = 566/610 (92.79%), Query Frame = 0

Query: 1   MTASPSP----LLDHLGFLNSTKSNFPNFNINNLKPITSQNS----IIHSALHSPSVLHF 60
           M ASP P    LLDHL F NST+SNFP FN NN     +QNS    IIHSALHSPSVLHF
Sbjct: 2   MAASPPPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHF 61

Query: 61  PNQTTTSTTTTSTTPNFHKSLSLKHQ--KSPTAPNWNLLQKTASMALDMVESALVSHELQ 120
           PNQ          TPNFH +L+LK Q  KSP + NWNLLQKTASMAL MVESALVSHELQ
Sbjct: 62  PNQ----------TPNFHNNLTLKDQTNKSPFS-NWNLLQKTASMALYMVESALVSHELQ 121

Query: 121 HPLPKTADPRVQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHL 180
           HPLPKTADPRVQISGNFAPVPEQ VKH LPV GTIPDCI+GVYLRNGANPLFQPTAGHHL
Sbjct: 122 HPLPKTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHL 181

Query: 181 FDGDGMVHAVSINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFY 240
           FDGDGMVHAV+IN GSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFY
Sbjct: 182 FDGDGMVHAVTINGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFY 241

Query: 241 ARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQL 300
           ARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDF+KQL
Sbjct: 242 ARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQL 301

Query: 301 HSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAI 360
           HSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSP+G+KSKDVEIPLETPTMMHDFAI
Sbjct: 302 HSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAI 361

Query: 361 TENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCF 420
           TENFVVIPDQQVVFKLQEMVKGGSPVIYDK KKSRFGILPKNATDSKDLIWVESPDTFCF
Sbjct: 362 TENFVVIPDQQVVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCF 421

Query: 421 HLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEE 480
           HLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEE
Sbjct: 422 HLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEE 481

Query: 481 KEQVNLEAGMVNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGE 540
           KEQ+NLEAGMVN+NR+GRK+R+AYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGE
Sbjct: 482 KEQMNLEAGMVNRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGE 541

Query: 541 PFFLPKEENSKEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTF 600
           PFFLPKE NSKEEDEGYILAFVHDERTWKSEVQIVNA DLKVEATIKLPSRVPYGFHGTF
Sbjct: 542 PFFLPKEGNSKEEDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTF 600

BLAST of CsaV3_6G051790 vs. NCBI nr
Match: XP_022926148.1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1035.0 bits (2675), Expect = 2.5e-298
Identity = 511/600 (85.17%), Postives = 550/600 (91.67%), Query Frame = 0

Query: 3   ASPS-PLLDHLGFLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQTTTSTT 62
           +SPS P LD+LGF NSTKS     N+N+++  ++ N++IHSALHSPSVLHFPN       
Sbjct: 4   SSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNH------ 63

Query: 63  TTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPRV 122
               +PNFH +L     KSP + NWN LQK+AS+ALDM ESAL+SHEL+HPLPKTADPRV
Sbjct: 64  ----SPNFHDNLI----KSPNS-NWNFLQKSASIALDMAESALLSHELRHPLPKTADPRV 123

Query: 123 QISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAVS 182
           QISGNFAPVPEQ VKH LPV GTIPDCINGVYLRNGANPLF+PTAGHHLFDGDGMVHAV+
Sbjct: 124 QISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVT 183

Query: 183 INHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHK 242
           IN GSASYACRFTQTQRL QE++LGRP+FPKAIGELHGHSGIARLLLFYARG+FGLVDHK
Sbjct: 184 INDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHK 243

Query: 243 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKVD 302
           KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDL+TVGRYDF+ QL STMIAHPKVD
Sbjct: 244 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVD 303

Query: 303 PISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQQ 362
           P+S+ELYALSYDVVRKPYLKYFRFSP+GIKSKDVEIPL+TPTMMHDFAITENFVVIPDQQ
Sbjct: 304 PVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQ 363

Query: 363 VVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPET 422
           VVFKLQEM+KGGSPVIYDKNKKSRFGILPKNAT+S DLIWVESPDTFCFHLWNAWEEPET
Sbjct: 364 VVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPET 423

Query: 423 EEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMV 482
           EEVVVIGSCMTPPDSIFNEC+ENLKSVLSEIRLNLRTGKSTRR II +EKEQVNLEAGMV
Sbjct: 424 EEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMV 483

Query: 483 NKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENSK 542
           N+NR+GRK+RYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL KE NS 
Sbjct: 484 NRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSG 543

Query: 543 E-EDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQNQA 601
           E EDEGYILAFVHDERTWKSE+QIVNA DLKVEAT+KLPSRVPYGFHGTFV +  LQNQA
Sbjct: 544 EKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

BLAST of CsaV3_6G051790 vs. ExPASy Swiss-Prot
Match: O24023 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED1 PE=1 SV=2)

HSP 1 Score: 892.9 bits (2306), Expect = 2.0e-258
Identity = 430/590 (72.88%), Postives = 499/590 (84.58%), Query Frame = 0

Query: 12  LGFLNSTKSNFPNFNINNLKPITSQNSI-IHSALHSPSVLHFPNQTTTSTTTTSTTPNFH 71
           L   +S +  F + +I+ LK   ++ S+ I+S+L +P +LHFP Q++   T  + T + H
Sbjct: 17  LSMPSSKEFGFASNSISLLKNQHNRQSLNINSSLQAPPILHFPKQSSNYQTPKNNTIS-H 76

Query: 72  KSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPRVQISGNFAPV 131
                 +  S +   WNL+QK A+MALD VESAL  HEL+HPLPKTADPRVQISGNFAPV
Sbjct: 77  PKQENNNSSSSSTSKWNLVQKAAAMALDAVESALTKHELEHPLPKTADPRVQISGNFAPV 136

Query: 132 PEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAVSINHGSASYA 191
           PE  V   LPV G IP C+ GVY+RNGANPLF+PTAGHH FDGDGMVHAV   +GSASYA
Sbjct: 137 PENPVCQSLPVTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYA 196

Query: 192 CRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAG 251
           CRFT+T+RLVQE+ LGRPVFPKAIGELHGHSGIARL+LFYARG+FGLVDH KGTGVANAG
Sbjct: 197 CRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSKGTGVANAG 256

Query: 252 LVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKVDPISKELYAL 311
           LVYFN+RLLAMSEDDLPYHV++TP+GDL+T GR+DFD QL STMIAHPK+DP+S EL+AL
Sbjct: 257 LVYFNNRLLAMSEDDLPYHVKVTPTGDLKTEGRFDFDGQLKSTMIAHPKLDPVSGELFAL 316

Query: 312 SYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQQVVFKLQEMV 371
           SYDV++KPYLKYFRFS NG KS DVEIP+E PTMMHDFAITENFVVIPDQQVVFK+ EM+
Sbjct: 317 SYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITENFVVIPDQQVVFKMSEMI 376

Query: 372 KGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSC 431
           +GGSPV+YDKNK SRFGIL K A D  DL WVE PD FCFHLWNAWEE ET+E+VVIGSC
Sbjct: 377 RGGSPVVYDKNKVSRFGILDKYAKDGSDLKWVEVPDCFCFHLWNAWEEAETDEIVVIGSC 436

Query: 432 MTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNKNRVGRKS 491
           MTPPDSIFNEC+E LKSVLSEIRLNL+TGKSTR++II+   EQVNLEAGMVN+N++GRK+
Sbjct: 437 MTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRKSIIENPDEQVNLEAGMVNRNKLGRKT 496

Query: 492 RYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENSKEEDEGYILA 551
            YAYLAIAEPWPKVSGFAKV+L TGEV+K IYG  K+GGEP FLP++ NSKEED+GYILA
Sbjct: 497 EYAYLAIAEPWPKVSGFAKVNLFTGEVEKFIYGDNKYGGEPLFLPRDPNSKEEDDGYILA 556

Query: 552 FVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQNQA 601
           FVHDE+ WKSE+QIVNA  LK+EAT+KLPSRVPYGFHGTF+ +NDL NQA
Sbjct: 557 FVHDEKEWKSELQIVNAMSLKLEATVKLPSRVPYGFHGTFINANDLANQA 605

BLAST of CsaV3_6G051790 vs. ExPASy Swiss-Prot
Match: K4CJJ1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 1.4e-256
Identity = 430/582 (73.88%), Postives = 501/582 (86.08%), Query Frame = 0

Query: 20  SNFPNFNI-NNLKPITSQNSIIHSALHSPSVLHFPNQTTTSTTTTSTTPNFHKSLSL-KH 79
           S   N+ + ++  P TS +  +   L S S+    N TTT T   S      K  +  K+
Sbjct: 3   STIANYRVSHSFSPSTSYS--LDFTLPSKSI-SMKNHTTTKTKIHSALLTLPKQNNTPKN 62

Query: 80  QKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPRVQISGNFAPVPEQAVKH 139
           Q      +WN  QK A+ ALD+VESALVS ELQ+PLPKTADPRVQI+GNFAPVPEQ+V+H
Sbjct: 63  QPQFQTSHWNFFQKAAAKALDIVESALVSRELQNPLPKTADPRVQIAGNFAPVPEQSVRH 122

Query: 140 YLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAVSINHGSASYACRFTQTQ 199
            LPV GTIPDCINGVY+RNGANPLF+P AGHHLFDGDGMVHAV++ +GS SY+CRFT+T+
Sbjct: 123 NLPVTGTIPDCINGVYVRNGANPLFEPVAGHHLFDGDGMVHAVTVENGSVSYSCRFTETE 182

Query: 200 RLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLVYFNDR 259
           RLVQER LG PVFPKAIGELHGHSGIARLLLFYARGVFGLVDH  GTGVANAGLV+FN+R
Sbjct: 183 RLVQERELGHPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHSHGTGVANAGLVFFNNR 242

Query: 260 LLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKVDPISKELYALSYDVVRK 319
           LLAMSEDD+PYHV++ PSGDLQTVGRY+FD QL STMIAHPK+DP+S EL+ALSYDVV+K
Sbjct: 243 LLAMSEDDVPYHVQVLPSGDLQTVGRYNFDDQLKSTMIAHPKIDPVSGELFALSYDVVQK 302

Query: 320 PYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVI 379
           PYLK F+FSP+G KS DVEIPL+ PTMMHDFAITEN+VVIPDQQVVFKLQEM+KGGSPVI
Sbjct: 303 PYLKSFKFSPDGEKSPDVEIPLDVPTMMHDFAITENYVVIPDQQVVFKLQEMIKGGSPVI 362

Query: 380 YDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSI 439
           YDKNKKSRFGILPKNA +S+++IWVES +TFCFHLWNAWEEPET+EV+VIGSCMTPPDSI
Sbjct: 363 YDKNKKSRFGILPKNAENSENIIWVESAETFCFHLWNAWEEPETDEVIVIGSCMTPPDSI 422

Query: 440 FNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNKNRVGRKSRYAYLAI 499
           FNEC ENLKSVLSEIRLNL+TG+STRR ++    +QVNLEAGMVN+N++GRK+++AYLAI
Sbjct: 423 FNECNENLKSVLSEIRLNLKTGESTRRQLL-SPSDQVNLEAGMVNRNKLGRKTQFAYLAI 482

Query: 500 AEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENSKEEDEGYILAFVHDERT 559
           AEPWPKVSGFAKVDL TGE+KKHIYG +++GGEP FLP+  NS++ED+GYILAF HDE+T
Sbjct: 483 AEPWPKVSGFAKVDLSTGEIKKHIYGDKRYGGEPLFLPRNVNSEKEDDGYILAFCHDEKT 542

Query: 560 WKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQNQ 600
           WKSE+QIVNA  L++EAT+KLPSRVPYGFHGTF+ S DLQNQ
Sbjct: 543 WKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQNQ 580

BLAST of CsaV3_6G051790 vs. ExPASy Swiss-Prot
Match: Q9M6E8 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 4.1e-243
Identity = 420/612 (68.63%), Postives = 493/612 (80.56%), Query Frame = 0

Query: 2   TASPSPLLDHLGFLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQ------ 61
           T  PS        L ST S+ P   +   K  +S  + I  +L +   LH+P Q      
Sbjct: 12  TTLPSSCSSPFKDLASTSSS-PTTLLPFKKRSSSNTNTITCSLQT---LHYPKQYQPTST 71

Query: 62  -TTTS------TTTTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHE 121
            TTT+      TTTT+TT    ++  L   K P    WN LQK A+  LDMVE+ALVSHE
Sbjct: 72  STTTTPTPIKPTTTTTTTTPHRETKPLSDTKQPFPQKWNFLQKAAATGLDMVETALVSHE 131

Query: 122 LQHPLPKTADPRVQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGH 181
            +HPLPKTADP+VQI+GNFAPVPE A    LPV G IP CI+GVY+RNGANPL++P AGH
Sbjct: 132 SKHPLPKTADPKVQIAGNFAPVPEHAADQALPVVGKIPKCIDGVYVRNGANPLYEPVAGH 191

Query: 182 HLFDGDGMVHAVSINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLL 241
           H FDGDGMVHAV   +G+ASYACRFT+TQRL QE+ LGRPVFPKAIGELHGHSGIARLLL
Sbjct: 192 HFFDGDGMVHAVKFTNGAASYACRFTETQRLAQEKSLGRPVFPKAIGELHGHSGIARLLL 251

Query: 242 FYARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDK 301
           FYAR +F LVD   G GVANAGLVYFN+ LLAMSEDDLPYHVRIT +GDL TVGRYDF+ 
Sbjct: 252 FYARSLFQLVDGSHGMGVANAGLVYFNNHLLAMSEDDLPYHVRITSNGDLTTVGRYDFNG 311

Query: 302 QLHSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDF 361
           QL+STMIAHPK+DP++ +L+ALSYDVV+KPYLKYFRFS +G+KS DVEIPL+ PTMMHDF
Sbjct: 312 QLNSTMIAHPKLDPVNGDLHALSYDVVQKPYLKYFRFSADGVKSPDVEIPLKEPTMMHDF 371

Query: 362 AITENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTF 421
           AITENFVV+PDQQVVFKL EM+ GGSPV+YDKNK SRFGIL KNA D+  + W+++P+ F
Sbjct: 372 AITENFVVVPDQQVVFKLTEMITGGSPVVYDKNKTSRFGILDKNAKDANAMRWIDAPECF 431

Query: 422 CFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIK 481
           CFHLWNAWEEPET+E+VVIGSCMTP DSIFNEC+E+LKSVLSEIRLNLRTGKSTRR II 
Sbjct: 432 CFHLWNAWEEPETDEIVVIGSCMTPADSIFNECDESLKSVLSEIRLNLRTGKSTRRPII- 491

Query: 482 EEKEQVNLEAGMVNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFG 541
            + EQVNLEAGMVN+N++GRK+++AYLA+AEPWPKVSGFAKVDL +GEV+K++YG  KFG
Sbjct: 492 SDAEQVNLEAGMVNRNKLGRKTQFAYLALAEPWPKVSGFAKVDLFSGEVQKYMYGEEKFG 551

Query: 542 GEPFFLPKEENSKEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHG 601
           GEP FLP   N +EE +GYILAFVHDE+ WKSE+QIVNA++LK+EA+IKLPSRVPYGFHG
Sbjct: 552 GEPLFLP---NGEEEGDGYILAFVHDEKEWKSELQIVNAQNLKLEASIKLPSRVPYGFHG 611

BLAST of CsaV3_6G051790 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 4.2e-240
Identity = 405/560 (72.32%), Postives = 461/560 (82.32%), Query Frame = 0

Query: 40  IHSALHSPSVLHFPNQTTTSTTTTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDM 99
           + SALH+P  LHFP Q++ S             +  K ++S T    NL Q+ A+ ALD 
Sbjct: 50  VSSALHTPPALHFPKQSSNSPAIV---------VKPKAKESNT-KQMNLFQRAAAAALDA 109

Query: 100 VESALVSHELQHPLPKTADPRVQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGAN 159
            E  LVSHE  HPLPKTADP VQI+GNFAPV EQ V+  LPV G +PD I GVY+RNGAN
Sbjct: 110 AEGFLVSHEKLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGAN 169

Query: 160 PLFQPTAGHHLFDGDGMVHAVSINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHG 219
           PL +P  GHH FDGDGMVHAV   HGSASYACRFTQT R VQER+LGRPVFPKAIGELHG
Sbjct: 170 PLHEPVTGHHFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHG 229

Query: 220 HSGIARLLLFYARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQ 279
           H+GIARL+LFYAR   G+VD   GTGVANAGLVYFN RLLAMSEDDLPY V+ITP+GDL+
Sbjct: 230 HTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLK 289

Query: 280 TVGRYDFDKQLHSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPL 339
           TVGR+DFD QL STMIAHPKVDP S EL+ALSYDVV KPYLKYFRFSP+G KS DVEI L
Sbjct: 290 TVGRFDFDGQLESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQL 349

Query: 340 ETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDL 399
           + PTMMHDFAITENFVV+PDQQVVFKL EM++GGSPV+YDKNK +RFGIL K A DS ++
Sbjct: 350 DQPTMMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNI 409

Query: 400 IWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTG 459
            W+++PD FCFHLWNAWEEPET+EVVVIGSCMTPPDSIFNE +ENLKSVLSEIRLNL+TG
Sbjct: 410 KWIDAPDCFCFHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTG 469

Query: 460 KSTRRAIIKEEKEQVNLEAGMVNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKK 519
           +STRR II  E +QVNLEAGMVN+N +GRK+++AYLA+AEPWPKVSGFAKVDL TGEVKK
Sbjct: 470 ESTRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKK 529

Query: 520 HIYGGRKFGGEPFFLPKEENSKEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLP 579
           H+YG  ++GGEP FLP E    EEDEGYIL FVHDE+TWKSE+QIVNA  L+VEAT+KLP
Sbjct: 530 HLYGDNRYGGEPLFLPGE--GGEEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLP 589

Query: 580 SRVPYGFHGTFVQSNDLQNQ 600
           SRVPYGFHGTF+ ++DL  Q
Sbjct: 590 SRVPYGFHGTFIGADDLAKQ 597

BLAST of CsaV3_6G051790 vs. ExPASy Swiss-Prot
Match: Q9C6Z1 (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED5 PE=1 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 2.6e-237
Identity = 397/555 (71.53%), Postives = 460/555 (82.88%), Query Frame = 0

Query: 53  PNQTTTSTTTTSTTPNFHKSLSLKHQKSPT-------APNWNLLQKTASMALDMVESALV 112
           P +   S  + S TPN    L+  +  SP           WN LQ+ AS ALD  E+AL+
Sbjct: 39  PRRRKLSANSVSDTPNL---LNFPNYPSPNPIIPEKDTSRWNPLQRAASAALDFAETALL 98

Query: 113 SHELQHPLPKTADPRVQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPT 172
             E   PLPKT DPR QISGN+APVPEQ+VK  L V G IPDCI+GVYLRNGANPLF+P 
Sbjct: 99  RRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPV 158

Query: 173 AGHHLFDGDGMVHAVSINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIAR 232
           +GHHLFDGDGMVHAV I +G ASY+CRFT+T+RLVQE++LG P+FPKAIGELHGHSGIAR
Sbjct: 159 SGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPIFPKAIGELHGHSGIAR 218

Query: 233 LLLFYARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYD 292
           L+LFYARG+FGL++HK GTGVANAGLVYF+DRLLAMSEDDLPY VR+T +GDL+T+GR+D
Sbjct: 219 LMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFD 278

Query: 293 FDKQLHSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMM 352
           FD QL S MIAHPK+DP++KEL+ALSYDVV+KPYLKYF+FSP G KS DVEIPL +PTMM
Sbjct: 279 FDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMM 338

Query: 353 HDFAITENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESP 412
           HDFAITENFVVIPDQQVVFKL +M  G SPV YD  K SRFGILP+NA D+ +++WVESP
Sbjct: 339 HDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKDASEMVWVESP 398

Query: 413 DTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRA 472
           +TFCFHLWNAWE PET+EVVVIGSCMTP DSIFNEC+E L SVLSEIRLNL+TGKSTRR 
Sbjct: 399 ETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRT 458

Query: 473 IIKEEKEQVNLEAGMVNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGR 532
           II     Q+NLEAGMVN+N +GRK+RYAYLAIAEPWPKVSGFAKVDL TGEVK H YGG+
Sbjct: 459 II-PGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAKVDLSTGEVKNHFYGGK 518

Query: 533 KFGGEPFFLPKEENSKEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYG 592
           K+GGEPFFLP+   S  ED+GYI++FVHDE +W+SE+ IVNA  L++EAT+KLPSRVPYG
Sbjct: 519 KYGGEPFFLPRGLESDGEDDGYIMSFVHDEESWESELHIVNAVTLELEATVKLPSRVPYG 578

Query: 593 FHGTFVQSNDLQNQA 601
           FHGTFV S D+ NQA
Sbjct: 579 FHGTFVNSADMLNQA 589

BLAST of CsaV3_6G051790 vs. ExPASy TrEMBL
Match: A0A0A0KIL6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G523440 PE=3 SV=1)

HSP 1 Score: 1223.4 bits (3164), Expect = 0.0e+00
Identity = 600/600 (100.00%), Postives = 600/600 (100.00%), Query Frame = 0

Query: 1   MTASPSPLLDHLGFLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQTTTST 60
           MTASPSPLLDHLGFLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQTTTST
Sbjct: 1   MTASPSPLLDHLGFLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQTTTST 60

Query: 61  TTTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPR 120
           TTTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPR
Sbjct: 61  TTTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPR 120

Query: 121 VQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV 180
           VQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV
Sbjct: 121 VQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV 180

Query: 181 SINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDH 240
           SINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDH
Sbjct: 181 SINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDH 240

Query: 241 KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV 300
           KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV
Sbjct: 241 KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV 300

Query: 301 DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQ 360
           DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQ
Sbjct: 301 DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQ 360

Query: 361 QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE 420
           QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE
Sbjct: 361 QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE 420

Query: 421 TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM 480
           TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM
Sbjct: 421 TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM 480

Query: 481 VNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENS 540
           VNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENS
Sbjct: 481 VNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENS 540

Query: 541 KEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQNQA 600
           KEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQNQA
Sbjct: 541 KEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQNQA 600

BLAST of CsaV3_6G051790 vs. ExPASy TrEMBL
Match: A0A1S3AZU0 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103484361 PE=3 SV=1)

HSP 1 Score: 1169.5 bits (3024), Expect = 0.0e+00
Identity = 577/603 (95.69%), Postives = 585/603 (97.01%), Query Frame = 0

Query: 1   MTASPSPLLDHLGFLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQTTTST 60
           MTA PSPLLDHLGFLNSTKSNFPN NIN+LK IT+QNSIIHSALHSPSVL FPNQ    T
Sbjct: 1   MTAPPSPLLDHLGFLNSTKSNFPNLNINHLKSITTQNSIIHSALHSPSVLRFPNQ----T 60

Query: 61  TTTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPR 120
           TTT   PNFHKSLSLKHQKSPTAPNWNLLQKTAS+ALDMVESALVSHELQHPLPKTADPR
Sbjct: 61  TTTPPPPNFHKSLSLKHQKSPTAPNWNLLQKTASIALDMVESALVSHELQHPLPKTADPR 120

Query: 121 VQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV 180
           VQISGNFAPVPEQ VKH LPV GTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV
Sbjct: 121 VQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV 180

Query: 181 SINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDH 240
           +INHGSASYACRFTQTQRLVQERRLGRP+FPKAIGELHGHSGIARLLLFYARGVFGLVDH
Sbjct: 181 TINHGSASYACRFTQTQRLVQERRLGRPIFPKAIGELHGHSGIARLLLFYARGVFGLVDH 240

Query: 241 KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV 300
           KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV
Sbjct: 241 KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV 300

Query: 301 DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQ 360
           DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIP+ETPTMMHDFAITENFVVIPDQ
Sbjct: 301 DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPVETPTMMHDFAITENFVVIPDQ 360

Query: 361 QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE 420
           QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE
Sbjct: 361 QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE 420

Query: 421 TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM 480
           TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM
Sbjct: 421 TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM 480

Query: 481 VNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENS 540
           VNK RVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPK+ENS
Sbjct: 481 VNKTRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKDENS 540

Query: 541 K---EEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQ 600
           K   EEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSN+LQ
Sbjct: 541 KAEEEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNNLQ 599

BLAST of CsaV3_6G051790 vs. ExPASy TrEMBL
Match: A0A5A7UDK3 (9-cis-epoxycarotenoid dioxygenase NCED3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G001390 PE=3 SV=1)

HSP 1 Score: 1166.4 bits (3016), Expect = 0.0e+00
Identity = 576/603 (95.52%), Postives = 584/603 (96.85%), Query Frame = 0

Query: 1   MTASPSPLLDHLGFLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQTTTST 60
           MTA PS LLDHLGFLNSTKSNFPN NIN+LK IT+QNSIIHSALHSPSVL FPNQ    T
Sbjct: 1   MTAPPSALLDHLGFLNSTKSNFPNLNINHLKSITTQNSIIHSALHSPSVLRFPNQ----T 60

Query: 61  TTTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPR 120
           TTT   PNFHKSLSLKHQKSPTAPNWNLLQKTAS+ALDMVESALVSHELQHPLPKTADPR
Sbjct: 61  TTTPPPPNFHKSLSLKHQKSPTAPNWNLLQKTASIALDMVESALVSHELQHPLPKTADPR 120

Query: 121 VQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV 180
           VQISGNFAPVPEQ VKH LPV GTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV
Sbjct: 121 VQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAV 180

Query: 181 SINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDH 240
           +INHGSASYACRFTQTQRLVQERRLGRP+FPKAIGELHGHSGIARLLLFYARGVFGLVDH
Sbjct: 181 TINHGSASYACRFTQTQRLVQERRLGRPIFPKAIGELHGHSGIARLLLFYARGVFGLVDH 240

Query: 241 KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV 300
           KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV
Sbjct: 241 KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKV 300

Query: 301 DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQ 360
           DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIP+ETPTMMHDFAITENFVVIPDQ
Sbjct: 301 DPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPVETPTMMHDFAITENFVVIPDQ 360

Query: 361 QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE 420
           QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE
Sbjct: 361 QVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE 420

Query: 421 TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM 480
           TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM
Sbjct: 421 TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGM 480

Query: 481 VNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENS 540
           VNK RVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPK+ENS
Sbjct: 481 VNKTRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKDENS 540

Query: 541 K---EEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQ 600
           K   EEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSN+LQ
Sbjct: 541 KAEEEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNNLQ 599

BLAST of CsaV3_6G051790 vs. ExPASy TrEMBL
Match: A0A6J1EH85 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111433338 PE=3 SV=1)

HSP 1 Score: 1035.0 bits (2675), Expect = 1.2e-298
Identity = 511/600 (85.17%), Postives = 550/600 (91.67%), Query Frame = 0

Query: 3   ASPS-PLLDHLGFLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQTTTSTT 62
           +SPS P LD+LGF NSTKS     N+N+++  ++ N++IHSALHSPSVLHFPN       
Sbjct: 4   SSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNH------ 63

Query: 63  TTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPRV 122
               +PNFH +L     KSP + NWN LQK+AS+ALDM ESAL+SHEL+HPLPKTADPRV
Sbjct: 64  ----SPNFHDNLI----KSPNS-NWNFLQKSASIALDMAESALLSHELRHPLPKTADPRV 123

Query: 123 QISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAVS 182
           QISGNFAPVPEQ VKH LPV GTIPDCINGVYLRNGANPLF+PTAGHHLFDGDGMVHAV+
Sbjct: 124 QISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVT 183

Query: 183 INHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHK 242
           IN GSASYACRFTQTQRL QE++LGRP+FPKAIGELHGHSGIARLLLFYARG+FGLVDHK
Sbjct: 184 INDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHK 243

Query: 243 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKVD 302
           KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDL+TVGRYDF+ QL STMIAHPKVD
Sbjct: 244 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVD 303

Query: 303 PISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQQ 362
           P+S+ELYALSYDVVRKPYLKYFRFSP+GIKSKDVEIPL+TPTMMHDFAITENFVVIPDQQ
Sbjct: 304 PVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQ 363

Query: 363 VVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPET 422
           VVFKLQEM+KGGSPVIYDKNKKSRFGILPKNAT+S DLIWVESPDTFCFHLWNAWEEPET
Sbjct: 364 VVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPET 423

Query: 423 EEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMV 482
           EEVVVIGSCMTPPDSIFNEC+ENLKSVLSEIRLNLRTGKSTRR II +EKEQVNLEAGMV
Sbjct: 424 EEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMV 483

Query: 483 NKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENSK 542
           N+NR+GRK+RYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL KE NS 
Sbjct: 484 NRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSG 543

Query: 543 E-EDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQNQA 601
           E EDEGYILAFVHDERTWKSE+QIVNA DLKVEAT+KLPSRVPYGFHGTFV +  LQNQA
Sbjct: 544 EKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

BLAST of CsaV3_6G051790 vs. ExPASy TrEMBL
Match: A0A6J1IVE0 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111478741 PE=3 SV=1)

HSP 1 Score: 1032.3 bits (2668), Expect = 7.9e-298
Identity = 509/600 (84.83%), Postives = 550/600 (91.67%), Query Frame = 0

Query: 3   ASPS-PLLDHLGFLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQTTTSTT 62
           +SPS P LD+LGF NSTKS     N+N+++  ++ N++IHSALHSPSVLHFPN       
Sbjct: 4   SSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNH------ 63

Query: 63  TTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHELQHPLPKTADPRV 122
               +PNFH +L     KSP + NWN LQK+AS+ALDM ESAL+SHEL+HPLPKTADPRV
Sbjct: 64  ----SPNFHDNLI----KSPNS-NWNFLQKSASIALDMAESALLSHELRHPLPKTADPRV 123

Query: 123 QISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAVS 182
           QISGNFAPVPEQ VK+ LPV GTIPDCINGVYLRNGANPLF+PTAGHHLFDGDGMVHAV+
Sbjct: 124 QISGNFAPVPEQPVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVT 183

Query: 183 INHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHK 242
           IN GSASYACRFTQTQRL QE++LGRP+FPKAIGELHGHSGIARLLLFYARG+FGLVDHK
Sbjct: 184 INDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHK 243

Query: 243 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKVD 302
           KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDL+TVGRYDF+ QL STMIAHPKVD
Sbjct: 244 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVD 303

Query: 303 PISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQQ 362
           P+S+ELYALSYDVVRKPYLKYFRFSP+GIKSKDVEIPL+TPTMMHDFAITENFVVIPDQQ
Sbjct: 304 PVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQ 363

Query: 363 VVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPET 422
           VVFKLQEM+KGGSPVIYDKNKKSRFGILPKNAT+S DLIWVESPDTFCFHLWNAWEEPET
Sbjct: 364 VVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPET 423

Query: 423 EEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMV 482
           EEVVVIGSCMTPPDSIFNEC+ENLKSVLSEIRLNLRTGKSTRR II +EKEQVNLEAGMV
Sbjct: 424 EEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMV 483

Query: 483 NKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENSK 542
           N+NR+GRK+RYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL KE NS 
Sbjct: 484 NRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSG 543

Query: 543 E-EDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQNQA 601
           E EDEGYILAFVHDERTWKSE+QIVNA DLKVEAT+KLPSRVPYGFHGTF+ +  LQNQA
Sbjct: 544 EKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 588

BLAST of CsaV3_6G051790 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 832.0 bits (2148), Expect = 3.0e-241
Identity = 405/560 (72.32%), Postives = 461/560 (82.32%), Query Frame = 0

Query: 40  IHSALHSPSVLHFPNQTTTSTTTTSTTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDM 99
           + SALH+P  LHFP Q++ S             +  K ++S T    NL Q+ A+ ALD 
Sbjct: 50  VSSALHTPPALHFPKQSSNSPAIV---------VKPKAKESNT-KQMNLFQRAAAAALDA 109

Query: 100 VESALVSHELQHPLPKTADPRVQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGAN 159
            E  LVSHE  HPLPKTADP VQI+GNFAPV EQ V+  LPV G +PD I GVY+RNGAN
Sbjct: 110 AEGFLVSHEKLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGAN 169

Query: 160 PLFQPTAGHHLFDGDGMVHAVSINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHG 219
           PL +P  GHH FDGDGMVHAV   HGSASYACRFTQT R VQER+LGRPVFPKAIGELHG
Sbjct: 170 PLHEPVTGHHFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHG 229

Query: 220 HSGIARLLLFYARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQ 279
           H+GIARL+LFYAR   G+VD   GTGVANAGLVYFN RLLAMSEDDLPY V+ITP+GDL+
Sbjct: 230 HTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLK 289

Query: 280 TVGRYDFDKQLHSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPL 339
           TVGR+DFD QL STMIAHPKVDP S EL+ALSYDVV KPYLKYFRFSP+G KS DVEI L
Sbjct: 290 TVGRFDFDGQLESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQL 349

Query: 340 ETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDL 399
           + PTMMHDFAITENFVV+PDQQVVFKL EM++GGSPV+YDKNK +RFGIL K A DS ++
Sbjct: 350 DQPTMMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNI 409

Query: 400 IWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTG 459
            W+++PD FCFHLWNAWEEPET+EVVVIGSCMTPPDSIFNE +ENLKSVLSEIRLNL+TG
Sbjct: 410 KWIDAPDCFCFHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTG 469

Query: 460 KSTRRAIIKEEKEQVNLEAGMVNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKK 519
           +STRR II  E +QVNLEAGMVN+N +GRK+++AYLA+AEPWPKVSGFAKVDL TGEVKK
Sbjct: 470 ESTRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKK 529

Query: 520 HIYGGRKFGGEPFFLPKEENSKEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLP 579
           H+YG  ++GGEP FLP E    EEDEGYIL FVHDE+TWKSE+QIVNA  L+VEAT+KLP
Sbjct: 530 HLYGDNRYGGEPLFLPGE--GGEEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLP 589

Query: 580 SRVPYGFHGTFVQSNDLQNQ 600
           SRVPYGFHGTF+ ++DL  Q
Sbjct: 590 SRVPYGFHGTFIGADDLAKQ 597

BLAST of CsaV3_6G051790 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 822.8 bits (2124), Expect = 1.8e-238
Identity = 397/555 (71.53%), Postives = 460/555 (82.88%), Query Frame = 0

Query: 53  PNQTTTSTTTTSTTPNFHKSLSLKHQKSPT-------APNWNLLQKTASMALDMVESALV 112
           P +   S  + S TPN    L+  +  SP           WN LQ+ AS ALD  E+AL+
Sbjct: 39  PRRRKLSANSVSDTPNL---LNFPNYPSPNPIIPEKDTSRWNPLQRAASAALDFAETALL 98

Query: 113 SHELQHPLPKTADPRVQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPT 172
             E   PLPKT DPR QISGN+APVPEQ+VK  L V G IPDCI+GVYLRNGANPLF+P 
Sbjct: 99  RRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPV 158

Query: 173 AGHHLFDGDGMVHAVSINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIAR 232
           +GHHLFDGDGMVHAV I +G ASY+CRFT+T+RLVQE++LG P+FPKAIGELHGHSGIAR
Sbjct: 159 SGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPIFPKAIGELHGHSGIAR 218

Query: 233 LLLFYARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYD 292
           L+LFYARG+FGL++HK GTGVANAGLVYF+DRLLAMSEDDLPY VR+T +GDL+T+GR+D
Sbjct: 219 LMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFD 278

Query: 293 FDKQLHSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMM 352
           FD QL S MIAHPK+DP++KEL+ALSYDVV+KPYLKYF+FSP G KS DVEIPL +PTMM
Sbjct: 279 FDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMM 338

Query: 353 HDFAITENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESP 412
           HDFAITENFVVIPDQQVVFKL +M  G SPV YD  K SRFGILP+NA D+ +++WVESP
Sbjct: 339 HDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKDASEMVWVESP 398

Query: 413 DTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRA 472
           +TFCFHLWNAWE PET+EVVVIGSCMTP DSIFNEC+E L SVLSEIRLNL+TGKSTRR 
Sbjct: 399 ETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRT 458

Query: 473 IIKEEKEQVNLEAGMVNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGR 532
           II     Q+NLEAGMVN+N +GRK+RYAYLAIAEPWPKVSGFAKVDL TGEVK H YGG+
Sbjct: 459 II-PGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAKVDLSTGEVKNHFYGGK 518

Query: 533 KFGGEPFFLPKEENSKEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYG 592
           K+GGEPFFLP+   S  ED+GYI++FVHDE +W+SE+ IVNA  L++EAT+KLPSRVPYG
Sbjct: 519 KYGGEPFFLPRGLESDGEDDGYIMSFVHDEESWESELHIVNAVTLELEATVKLPSRVPYG 578

Query: 593 FHGTFVQSNDLQNQA 601
           FHGTFV S D+ NQA
Sbjct: 579 FHGTFVNSADMLNQA 589

BLAST of CsaV3_6G051790 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 804.7 bits (2077), Expect = 5.1e-233
Identity = 391/571 (68.48%), Postives = 469/571 (82.14%), Query Frame = 0

Query: 31  KPITSQNSIIHSALHSPSVLHFPNQTTTSTTTTSTTPNFHKSLSLKHQKSPTAPNWNLLQ 90
           +PI  Q  +I   +    V     +    T  T+ TP  H  L L           N+ Q
Sbjct: 26  RPIKRQPKVIKCTVQI-DVTELTKKRQLFTPRTTATPPQHNPLRL-----------NIFQ 85

Query: 91  KTASMALDMVESALVSHELQHPLPKTADPRVQISGNFAPVPEQAVKHYLPVAGTIPDCIN 150
           K A++A+D  E AL+SHE   PLPKTADPRVQI+GN++PVPE +V+  L V GTIPDCI+
Sbjct: 86  KAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTVEGTIPDCID 145

Query: 151 GVYLRNGANPLFQPTAGHHLFDGDGMVHAVSINHGSASYACRFTQTQRLVQERRLGRPVF 210
           GVY+RNGANP+F+PTAGHHLFDGDGMVHAV I +GSASYACRFT+T+RLVQE+RLGRPVF
Sbjct: 146 GVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITNGSASYACRFTKTERLVQEKRLGRPVF 205

Query: 211 PKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHV 270
           PKAIGELHGHSGIARL+LFYARG+ GL++++ G GVANAGLVYFN+RLLAMSEDDLPY +
Sbjct: 206 PKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAMSEDDLPYQL 265

Query: 271 RITPSGDLQTVGRYDFDKQLHSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSPNGI 330
           +IT +GDLQTVGRYDFD QL S MIAHPK+DP++KEL+ALSYDVV+KPYLKYFRFSP+G+
Sbjct: 266 KITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHALSYDVVKKPYLKYFRFSPDGV 325

Query: 331 KSKDVEIPLETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILP 390
           KS ++EIPLETPTM+HDFAITENFVVIPDQQVVFKL EM+ G SPV++D  K SR GI+P
Sbjct: 326 KSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMISGKSPVVFDGEKVSRLGIMP 385

Query: 391 KNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLS 450
           K+AT++  +IWV SP+TFCFHLWNAWE PETEE+VVIGSCM+P DSIFNE +E+L+SVLS
Sbjct: 386 KDATEASQIIWVNSPETFCFHLWNAWESPETEEIVVIGSCMSPADSIFNERDESLRSVLS 445

Query: 451 EIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNKNRVGRKSRYAYLAIAEPWPKVSGFAKV 510
           EIR+NLRT K+TRR+++    E VNLE GMVN+NR+GRK+R+A+LAIA PWPKVSGFAKV
Sbjct: 446 EIRINLRTRKTTRRSLL--VNEDVNLEIGMVNRNRLGRKTRFAFLAIAYPWPKVSGFAKV 505

Query: 511 DLVTGEVKKHIYGGRKFGGEPFFLPKEENSKE--EDEGYILAFVHDERTWKSEVQIVNAK 570
           DL TGE+KK+IYGG K+GGEPFFLP    + E  ED+GYI   VHDE T  SE+QI+NA 
Sbjct: 506 DLCTGEMKKYIYGGEKYGGEPFFLPGNSGNGEENEDDGYIFCHVHDEETKTSELQIINAV 565

Query: 571 DLKVEATIKLPSRVPYGFHGTFVQSNDLQNQ 600
           +LK+EATIKLPSRVPYGFHGTFV SN+L +Q
Sbjct: 566 NLKLEATIKLPSRVPYGFHGTFVDSNELVDQ 582

BLAST of CsaV3_6G051790 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 776.9 bits (2005), Expect = 1.1e-224
Identity = 383/597 (64.15%), Postives = 468/597 (78.39%), Query Frame = 0

Query: 14  FLNSTKSNFPNFNINNLKPITSQNSIIHSALHSPSVLHFPNQTTTSTTTTSTTPNF---- 73
           FL+ T S   + +   L+ ++  +++ +  L  P  +       +S +++  +P F    
Sbjct: 64  FLDRTFSTSSSSSRPKLQSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPTFKPPS 123

Query: 74  -----HKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALVSHE-LQHPLPKTADPRVQI 133
                +   +L  + +   P  N +Q+TA+M LD VE+A++SHE  +HP PKTADP VQI
Sbjct: 124 WKKLCNDVTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQI 183

Query: 134 SGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAVSIN 193
           +GNF PVPE+ V H LPV GT+P+CI GVY+RNGANPL +P +GHHLFDGDGMVHAV  +
Sbjct: 184 AGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRFD 243

Query: 194 HGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKG 253
           +GS SYACRFT+T RLVQER  GRPVFPKAIGELHGH GIA+L+LF  RG+FGLVD   G
Sbjct: 244 NGSVSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGG 303

Query: 254 TGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKVDPI 313
            GVANAGLVYFN  LLAMSEDDLPYHV++T +GDL+T GRYDFD QL STMIAHPK+DP 
Sbjct: 304 LGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPE 363

Query: 314 SKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQQVV 373
           ++EL+ALSYDVV KPYLKYFRF+ +G KS DVEIPL+ PTM+HDFAITENFVVIPDQQVV
Sbjct: 364 TRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVV 423

Query: 374 FKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEE 433
           F+L EM++GGSPV+YD+ KKSRFGIL KNA D+  + W+E PD FCFHLWN+WEEPET+E
Sbjct: 424 FRLPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDE 483

Query: 434 VVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNK 493
           VVVIGSCMTPPDSIFNE +E L+SVLSEIRLNL+TG+STRR +I    EQVNLEAGMVN+
Sbjct: 484 VVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVI---SEQVNLEAGMVNR 543

Query: 494 NRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEENSKEE 553
           N +GRK+RYAYLA+ EPWPKVSGFAKVDL TGE++K+IYG  K+GGEP FLP  +   EE
Sbjct: 544 NLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLPSGDG--EE 603

Query: 554 DEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQNQA 601
           D GYI+ FVHDE   KSE+Q++NA ++K+EAT+ LPSRVPYGFHGTF+   DL  QA
Sbjct: 604 DGGYIMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQA 655

BLAST of CsaV3_6G051790 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 639.0 bits (1647), Expect = 3.7e-183
Identity = 318/541 (58.78%), Postives = 397/541 (73.38%), Query Frame = 0

Query: 65  TTPNFHKSLSLKHQKSPTAPNWNLLQKTASMALDMVESALV-SHELQHPLPKTADPRVQI 124
           T P+    +    +  PT PN NLLQK A+  LD +ES++V   E   PLPK  DP VQ+
Sbjct: 42  TLPDLTSPVPSPVKLKPTYPNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQL 101

Query: 125 SGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAVSIN 184
           SGNFAPV E  V++ L V G IP C+ GVY+RNGANP+F P AGHHLFDGDGM+HAVSI 
Sbjct: 102 SGNFAPVNECPVQNGLEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIG 161

Query: 185 -HGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKK 244
                SY+CR+T+T RLVQE  LGR VFPK IGELHGHSG+ARL LF AR   GLVD  +
Sbjct: 162 FDNQVSYSCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTR 221

Query: 245 GTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKVDP 304
           G GVANAG+V+FN RLLAMSEDDLPY V+I   GDL+T+GR+ FD Q+ S++IAHPKVD 
Sbjct: 222 GMGVANAGVVFFNGRLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDA 281

Query: 305 ISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENFVVIPDQQV 364
            + +L+ LSY+V++KP+L+Y +F+  G K++DVEI L  PTM+HDFAITENFVVIPDQQ+
Sbjct: 282 TTGDLHTLSYNVLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQM 341

Query: 365 VFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETE 424
           VFKL EM++GGSPVIY K K +RFG+L K      D+ WV+ PD FCFHLWNAWEE  TE
Sbjct: 342 VFKLSEMIRGGSPVIYVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEE-RTE 401

Query: 425 E----VVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEA 484
           E    +VVIGSCM+PPD+IF+E  E  +  LSEIRLN+RT +S R+ I+      VNLEA
Sbjct: 402 EGDPVIVVIGSCMSPPDTIFSESGEPTRVELSEIRLNMRTKESNRKVIV----TGVNLEA 461

Query: 485 GMVNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEE 544
           G +N++ VGRKS++ Y+AIA+PWPK SG AKVD+  G V +  YG  +FGGEP F+P+ E
Sbjct: 462 GHINRSYVGRKSQFVYIAIADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPEGE 521

Query: 545 NSKEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNDLQN 600
              EED+GY++ FV DE   +SE  +V+A D+K  A ++LP RVPYGFHGTFV  N L+ 
Sbjct: 522 G--EEDKGYVMGFVRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKE 575

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004134911.10.0e+00100.009-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic [Cucumis sativus] >KGN494... [more]
XP_008439625.10.0e+0095.69PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis ... [more]
KAA0052537.10.0e+0095.529-cis-epoxycarotenoid dioxygenase NCED3 [Cucumis melo var. makuwa][more]
XP_038883946.10.0e+0090.339-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic-like [Benincasa hispida][more]
XP_022926148.12.5e-29885.179-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O240232.0e-25872.889-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum O... [more]
K4CJJ11.4e-25673.889-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum O... [more]
Q9M6E84.1e-24368.639-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=... [more]
Q9LRR74.2e-24072.329-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9C6Z12.6e-23771.53Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis t... [more]
Match NameE-valueIdentityDescription
A0A0A0KIL60.0e+00100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G523440 PE=3 SV=1[more]
A0A1S3AZU00.0e+0095.699-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucumis melo OX=3... [more]
A0A5A7UDK30.0e+0095.529-cis-epoxycarotenoid dioxygenase NCED3 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A6J1EH851.2e-29885.179-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschat... [more]
A0A6J1IVE07.9e-29884.839-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima ... [more]
Match NameE-valueIdentityDescription
AT3G14440.13.0e-24172.32nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT1G30100.11.8e-23871.53nine-cis-epoxycarotenoid dioxygenase 5 [more]
AT4G18350.15.1e-23368.48nine-cis-epoxycarotenoid dioxygenase 2 [more]
AT1G78390.11.1e-22464.15nine-cis-epoxycarotenoid dioxygenase 9 [more]
AT3G24220.13.7e-18358.78nine-cis-epoxycarotenoid dioxygenase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 127..591
e-value: 3.4E-117
score: 392.5
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 52..600
NoneNo IPR availablePANTHERPTHR10543:SF1239-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATEDcoord: 52..600

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_6G051790.1CsaV3_6G051790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen