CsaV3_6G041420 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_6G041420
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionCysteine proteinase inhibitor 5-like
Locationchr6: 24089728 .. 24090060 (-)
RNA-Seq ExpressionCsaV3_6G041420
SyntenyCsaV3_6G041420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCTCATGTAATTTTTGGAGGTTATCGACCATGTGAGAATTCAGATGGTCCACATGCGAAAGAAGTAGCACAATGGGCAGTAACAGAATACAACATAAAACACCGCCATGAACGTCCTTACTTGTACCTTCTTAGTGTATTGAAGTGCGAGTCGCAAGTGGTGGCTGGAACCAATTGGCGTCTGGGGTTGAAGTGTAAGGATGAAAATAATATTGAGGTAAACTGTGAGGCTGTTGTGTGGGAGAAGAAGATGGGAGAATTTCAGGGAGCTCACATCCTTCATAGTATTCTACCCATCTTCTGGTTGACAAGGGATATCCCCTTGTAA

mRNA sequence

ATGTCTTCTCATGTAATTTTTGGAGGTTATCGACCATGTGAGAATTCAGATGGTCCACATGCGAAAGAAGTAGCACAATGGGCAGTAACAGAATACAACATAAAACACCGCCATGAACGTCCTTACTTGTACCTTCTTAGTGTATTGAAGTGCGAGTCGCAAGTGGTGGCTGGAACCAATTGGCGTCTGGGGTTGAAGTGTAAGGATGAAAATAATATTGAGGTAAACTGTGAGGCTGTTGTGTGGGAGAAGAAGATGGGAGAATTTCAGGGAGCTCACATCCTTCATAGTATTCTACCCATCTTCTGGTTGACAAGGGATATCCCCTTGTAA

Coding sequence (CDS)

ATGTCTTCTCATGTAATTTTTGGAGGTTATCGACCATGTGAGAATTCAGATGGTCCACATGCGAAAGAAGTAGCACAATGGGCAGTAACAGAATACAACATAAAACACCGCCATGAACGTCCTTACTTGTACCTTCTTAGTGTATTGAAGTGCGAGTCGCAAGTGGTGGCTGGAACCAATTGGCGTCTGGGGTTGAAGTGTAAGGATGAAAATAATATTGAGGTAAACTGTGAGGCTGTTGTGTGGGAGAAGAAGATGGGAGAATTTCAGGGAGCTCACATCCTTCATAGTATTCTACCCATCTTCTGGTTGACAAGGGATATCCCCTTGTAA

Protein sequence

MSSHVIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTNWRLGLKCKDENNIEVNCEAVVWEKKMGEFQGAHILHSILPIFWLTRDIPL*
Homology
BLAST of CsaV3_6G041420 vs. NCBI nr
Match: KAE8647449.1 (hypothetical protein Csa_002967 [Cucumis sativus])

HSP 1 Score: 243.4 bits (620), Expect = 9.0e-61
Identity = 110/110 (100.00%), Postives = 110/110 (100.00%), Query Frame = 0

Query: 1   MSSHVIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60
           MSSHVIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN
Sbjct: 1   MSSHVIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60

Query: 61  WRLGLKCKDENNIEVNCEAVVWEKKMGEFQGAHILHSILPIFWLTRDIPL 111
           WRLGLKCKDENNIEVNCEAVVWEKKMGEFQGAHILHSILPIFWLTRDIPL
Sbjct: 61  WRLGLKCKDENNIEVNCEAVVWEKKMGEFQGAHILHSILPIFWLTRDIPL 110

BLAST of CsaV3_6G041420 vs. NCBI nr
Match: KGN44121.2 (hypothetical protein Csa_021373 [Cucumis sativus])

HSP 1 Score: 189.1 bits (479), Expect = 2.0e-44
Identity = 85/89 (95.51%), Postives = 86/89 (96.63%), Query Frame = 0

Query: 1  MSSHVIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60
          MSSHVIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN
Sbjct: 1  MSSHVIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60

Query: 61 WRLGLKCKDENNIEVNCEAVVWEKKMGEF 90
          WRLGLKCKDENNIEVNCEAVVWEK+   F
Sbjct: 61 WRLGLKCKDENNIEVNCEAVVWEKRWENF 89

BLAST of CsaV3_6G041420 vs. NCBI nr
Match: KAE8645927.1 (hypothetical protein Csa_021385 [Cucumis sativus])

HSP 1 Score: 178.7 bits (452), Expect = 2.7e-41
Identity = 80/90 (88.89%), Postives = 83/90 (92.22%), Query Frame = 0

Query: 1  MSSHVIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60
          MSSHVI G YRPCENSDGPHAKEVAQWAVTEYN+KHRHERPYLYLLSVLKCESQVV GTN
Sbjct: 1  MSSHVIVGDYRPCENSDGPHAKEVAQWAVTEYNLKHRHERPYLYLLSVLKCESQVVGGTN 60

Query: 61 WRLGLKCKDENNIEVNCEAVVWEKKMGEFQ 91
          WRL LKCKDENNIEVNCEAVVWEK+   F+
Sbjct: 61 WRLELKCKDENNIEVNCEAVVWEKEWENFK 90

BLAST of CsaV3_6G041420 vs. NCBI nr
Match: KAE8645925.1 (hypothetical protein Csa_021376 [Cucumis sativus])

HSP 1 Score: 177.6 bits (449), Expect = 6.1e-41
Identity = 80/90 (88.89%), Postives = 82/90 (91.11%), Query Frame = 0

Query: 1  MSSHVIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60
          MSSHV  G Y PCENSDG HAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN
Sbjct: 1  MSSHVTLGAYGPCENSDGEHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60

Query: 61 WRLGLKCKDENNIEVNCEAVVWEKKMGEFQ 91
          WRLGLKCKDENNIEVNCEAVVWEK+   F+
Sbjct: 61 WRLGLKCKDENNIEVNCEAVVWEKRWENFR 90

BLAST of CsaV3_6G041420 vs. NCBI nr
Match: KAE8645926.1 (hypothetical protein Csa_021381 [Cucumis sativus])

HSP 1 Score: 174.9 bits (442), Expect = 4.0e-40
Identity = 80/90 (88.89%), Postives = 82/90 (91.11%), Query Frame = 0

Query: 1  MSSHVIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60
          MSS  I GGY PCENSDG HAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN
Sbjct: 1  MSSDSIKGGYGPCENSDGEHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60

Query: 61 WRLGLKCKDENNIEVNCEAVVWEKKMGEFQ 91
          WRLGLKCKDENNIEVNCEAVVWEK+   F+
Sbjct: 61 WRLGLKCKDENNIEVNCEAVVWEKRWENFK 90

BLAST of CsaV3_6G041420 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 5.8e-10
Identity = 37/86 (43.02%), Postives = 53/86 (61.63%), Query Frame = 0

Query: 5   VIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTNWRLG 64
           V  GG+RP EN +    ++VAQ+AV+E+N +   E  Y    SV++  +QVVAGTN+RL 
Sbjct: 27  VAAGGWRPIENLNSAEVQDVAQFAVSEHNKQANDELQY---QSVVRGYTQVVAGTNYRLV 86

Query: 65  LKCKDENNIEVNCEAVVWEKKMGEFQ 91
           +  KD   +  N EAVVW+K    F+
Sbjct: 87  IAAKD-GAVVGNYEAVVWDKPWMHFR 108

BLAST of CsaV3_6G041420 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.2e-09
Identity = 36/86 (41.86%), Postives = 53/86 (61.63%), Query Frame = 0

Query: 5   VIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTNWRLG 64
           V  GG+RP E+ +    ++VAQ+AV+E+N +   E  Y    SV++  +QVVAGTN+RL 
Sbjct: 27  VAAGGWRPIESLNSAEVQDVAQFAVSEHNKQANDELQY---QSVVRGYTQVVAGTNYRLV 86

Query: 65  LKCKDENNIEVNCEAVVWEKKMGEFQ 91
           +  KD   +  N EAVVW+K    F+
Sbjct: 87  IAAKD-GAVVGNYEAVVWDKPWMHFR 108

BLAST of CsaV3_6G041420 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 3.2e-08
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 0

Query: 8   GGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTNWRLGLKC 67
           GG+ P  N   P   E+ ++AV+EYN   +     L   +V+  E+QVV+GTN+RL +  
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYN---KRSESGLKFETVVSGETQVVSGTNYRLKVAA 88

Query: 68  KDENNIEVNCEAVVWEKKMGEFQ 91
            D + +  N  A+VW+K   +F+
Sbjct: 89  NDGDGVSKNYLAIVWDKPWMKFR 108

BLAST of CsaV3_6G041420 vs. ExPASy Swiss-Prot
Match: B7PKZ1 (Salivary cystatin-L2 OS=Ixodes scapularis OX=6945 GN=IscW_ISCW018602 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 3.3e-05
Identity = 24/59 (40.68%), Postives = 36/59 (61.02%), Query Frame = 0

Query: 8  GGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTNWRLGLK 67
          GGYR   N D P   E+A +A + ++ +   +  +  ++ VLK E+Q VAGTN+RL LK
Sbjct: 24 GGYRERSNQDDPEYLELAHYATSTWSAQQPGKTHFDTVVEVLKVETQTVAGTNYRLTLK 82

BLAST of CsaV3_6G041420 vs. ExPASy Swiss-Prot
Match: Q84WT8 (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=2)

HSP 1 Score: 45.1 bits (105), Expect = 6.2e-04
Identity = 26/77 (33.77%), Postives = 41/77 (53.25%), Query Frame = 0

Query: 8   GGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTNWRLGLKC 67
           G  +P +N   P    VA++A+ E+N   +  +  L  + V++  +QVV+GT + L +  
Sbjct: 30  GSRKPIKNVSDPDVVAVAKYAIEEHN---KESKEKLVFVKVVEGTTQVVSGTKYDLKIAA 89

Query: 68  KDENNIEVNCEAVVWEK 85
           KD      N EAVV EK
Sbjct: 90  KDGGGKIKNYEAVVVEK 103

BLAST of CsaV3_6G041420 vs. ExPASy TrEMBL
Match: A0A0A0KHK7 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G459990 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 3.0e-41
Identity = 80/90 (88.89%), Postives = 82/90 (91.11%), Query Frame = 0

Query: 1  MSSHVIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60
          MSSHV  G Y PCENSDG HAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN
Sbjct: 1  MSSHVTLGAYGPCENSDGEHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60

Query: 61 WRLGLKCKDENNIEVNCEAVVWEKKMGEFQ 91
          WRLGLKCKDENNIEVNCEAVVWEK+   F+
Sbjct: 61 WRLGLKCKDENNIEVNCEAVVWEKRWENFR 90

BLAST of CsaV3_6G041420 vs. ExPASy TrEMBL
Match: A0A0A0KF17 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G460000 PE=4 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.3e-40
Identity = 80/89 (89.89%), Postives = 81/89 (91.01%), Query Frame = 0

Query: 1  MSSHVIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60
          MSS  I GGY PCENSDG HAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN
Sbjct: 1  MSSDSIKGGYGPCENSDGEHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60

Query: 61 WRLGLKCKDENNIEVNCEAVVWEKKMGEF 90
          WRLGLKCKDENNIEVNCEAVVWEK+   F
Sbjct: 61 WRLGLKCKDENNIEVNCEAVVWEKRWENF 89

BLAST of CsaV3_6G041420 vs. ExPASy TrEMBL
Match: O80389 (Cystein proteinase inhibitor OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 9.9e-21
Identity = 54/90 (60.00%), Postives = 64/90 (71.11%), Query Frame = 0

Query: 1  MSSHVIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60
          MSS  I GGY PC++ + PH K++A+WAV EYN    H   +L L+S+LKCESQVVAG N
Sbjct: 1  MSSSEI-GGYVPCKDPNDPHVKDIAEWAVAEYNKSQGH---HLTLVSILKCESQVVAGVN 60

Query: 61 WRLGLKCKDENNIEVNCEAVVWEKKMGEFQ 91
          WRL LKCKDENN E N E VVWEK    F+
Sbjct: 61 WRLVLKCKDENNGEGNYETVVWEKIWENFR 86

BLAST of CsaV3_6G041420 vs. ExPASy TrEMBL
Match: A0A5A7TBL3 (Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold529G00310 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 2.4e-19
Identity = 52/89 (58.43%), Postives = 63/89 (70.79%), Query Frame = 0

Query: 1  MSSHVIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60
          MSS++  GGY PC+ S   HAK++ QWAV E+N +  H    L  +S+LKCESQVV GTN
Sbjct: 1  MSSYL--GGYVPCQESQSEHAKKLCQWAVAEHNKQGHH----LTFVSLLKCESQVVDGTN 60

Query: 61 WRLGLKCKDENNIEVNCEAVVWEKKMGEF 90
          WRL +KCKDENN E N EAVVWEK +  F
Sbjct: 61 WRLVMKCKDENNYEGNYEAVVWEKILEGF 83

BLAST of CsaV3_6G041420 vs. ExPASy TrEMBL
Match: A0A0A0KHN6 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G482230 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 5.6e-16
Identity = 50/102 (49.02%), Postives = 63/102 (61.76%), Query Frame = 0

Query: 1   MSSHVIFGGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60
           M SHV  G Y PCE+ +  H K +A+WAV EYN K  H   +L L+S+LKCESQVV GTN
Sbjct: 1   MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYN-KGDH---FLSLVSILKCESQVVNGTN 60

Query: 61  WRLGLKCKDENNIEVNC-EAVVWEKKMGEFQGAHILHSILPI 102
           WRL LKC D+ N  +     VVWEK      G+ +L   +P+
Sbjct: 61  WRLKLKCVDQINNRLGIYVTVVWEK----LDGSLVLSDFVPL 94

BLAST of CsaV3_6G041420 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 2.3e-09
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 0

Query: 8   GGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTNWRLGLKC 67
           GG+ P  N   P   E+ ++AV+EYN   +     L   +V+  E+QVV+GTN+RL +  
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYN---KRSESGLKFETVVSGETQVVSGTNYRLKVAA 88

Query: 68  KDENNIEVNCEAVVWEKKMGEFQ 91
            D + +  N  A+VW+K   +F+
Sbjct: 89  NDGDGVSKNYLAIVWDKPWMKFR 108

BLAST of CsaV3_6G041420 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 45.1 bits (105), Expect = 4.4e-05
Identity = 26/77 (33.77%), Postives = 41/77 (53.25%), Query Frame = 0

Query: 8   GGYRPCENSDGPHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTNWRLGLKC 67
           G  +P +N   P    VA++A+ E+N   +  +  L  + V++  +QVV+GT + L +  
Sbjct: 30  GSRKPIKNVSDPDVVAVAKYAIEEHN---KESKEKLVFVKVVEGTTQVVSGTKYDLKIAA 89

Query: 68  KDENNIEVNCEAVVWEK 85
           KD      N EAVV EK
Sbjct: 90  KDGGGKIKNYEAVVVEK 103

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8647449.19.0e-61100.00hypothetical protein Csa_002967 [Cucumis sativus][more]
KGN44121.22.0e-4495.51hypothetical protein Csa_021373 [Cucumis sativus][more]
KAE8645927.12.7e-4188.89hypothetical protein Csa_021385 [Cucumis sativus][more]
KAE8645925.16.1e-4188.89hypothetical protein Csa_021376 [Cucumis sativus][more]
KAE8645926.14.0e-4088.89hypothetical protein Csa_021381 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
P864725.8e-1043.02Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841... [more]
Q6TPK42.2e-0941.86Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1[more]
Q419163.2e-0834.94Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=... [more]
B7PKZ13.3e-0540.68Salivary cystatin-L2 OS=Ixodes scapularis OX=6945 GN=IscW_ISCW018602 PE=1 SV=1[more]
Q84WT86.2e-0433.77Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0KHK73.0e-4188.89Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G459990 PE... [more]
A0A0A0KF173.3e-4089.89Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G460000 PE... [more]
O803899.9e-2160.00Cystein proteinase inhibitor OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
A0A5A7TBL32.4e-1958.43Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A0A0KHN65.6e-1649.02Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G482230 PE... [more]
Match NameE-valueIdentityDescription
AT5G47550.12.3e-0934.94Cystatin/monellin superfamily protein [more]
AT4G16500.14.4e-0533.77Cystatin/monellin superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.450.10coord: 2..95
e-value: 1.7E-18
score: 68.6
NoneNo IPR availablePANTHERPTHR47364:SF2CYSTEINE PROTEINASE INHIBITOR 5coord: 3..90
NoneNo IPR availablePANTHERPTHR47364CYSTEINE PROTEINASE INHIBITOR 5coord: 3..90
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 3..86
IPR000010Cystatin domainPFAMPF16845SQAPIcoord: 15..90
e-value: 3.8E-16
score: 59.2
IPR000010Cystatin domainCDDcd00042CYcoord: 8..84
e-value: 9.69068E-7
score: 42.076
IPR018073Proteinase inhibitor I25, cystatin, conserved sitePROSITEPS00287CYSTATINcoord: 53..66

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_6G041420.1CsaV3_6G041420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity