CsaV3_6G031950 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_6G031950
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionAsparagine synthetase [glutamine-hydrolyzing]
Locationchr6: 18076105 .. 18080604 (+)
RNA-Seq ExpressionCsaV3_6G031950
SyntenyCsaV3_6G031950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTGGCCTTGTCTTTCCAACGATTTTACGTCTTAATCTCTGCGATTTGTTTTCCATTTTCCTTATTAATTCTCACTTATTTTCCTCTCCTATTTTTTCTCTTTCAATCAGATTTCCTCTGTTTTTTTTGCCTTTTGCTTTGCTCAATATGTGTGGGATTCTAGCCGTTCTTGGTTGCTCCGATGATTCTCAGGCCAAACGTGTTCGTGTTCTTGAACTCTCTCGTCGGCAAGTGTTCTTAATCTATAAAACTACCAATGATTTCCTAAAACTCCATTTCTGTTTTTATTTTTTATTTTTATTTTATATCGGGGCTATTCTTTTGTACTTTCGATCCTATTGAAGTTGTAAATATTGTTTGATATGTAGAATGGATTTAAGTCGTTTGTGTGTTTTTCATATTAAACAGGGAGAAGACTTGAAGTTCCAAGAATTATTTATGGTAGTTACATAATTTCAAAATGGAGTTTAAAAAGTTTGTTTTTTTTAGTTTTTTTTTTAAAAGTAATATGATTGTTAAATAGTTTACTGGTAAGCAATTCTTATGATACTTTGTTGTAACTTTGATTGTGACCTTTCAACTTTCCTTTTTCTAAAAAATCACATAGCATGATCTCGTTATAACTTAATAGACACTATTTTTTACCTTCAAATTTGTTATATAAAACACTTCATCTTTATGGTAGTTACATAATTTCAAGGTAAGATATGCAGAGAAAATACACTTAATCTTCACAAAATATATATAAAACAGAATGTTACCCCATGAAATCATTGATCAGCATTGGTTGATAGAAGAACTTTTAATATATAACTGATATCAATGGACTATTTCTTGTTGTTGATTTGACAGATTGAAACACCGAGGCCCAGACTGGAGTGGGCTTTACCAACACGCTGATTGCTATTTGTCCCATCAACGTTTAGCCATTGTCGATCCTGCATCCGGTGATCAACCTCTCTATAATGAAAATAAATCCATCGTTGTCACGGTACACTCCCCAACCTTCTTCGTTCGTATCTGATACCATGTAATTCCATCTCTCAACATATCCATTGAGATGGGGTCTTTTTTGAGTTCACCATTCTCGACCCGATTTTATGGTCGTTCTCATTCCATGTCAAAATCTAACTCATGACTGTAATTCTGAATGGTTGATCTTCTTCCCACCCACCCTCAAGTTTAATTTATTTGCTAGGGTGTGTTTTGTTTCGAATAAGCCACACACGAATACAAACTCTATCTTATCGTGCTAATGTGCAAAAGTTTTTGCTTGGAAACAGGTCAATGGAGAAATATACAACCACGAGGAGCTGAGGAAGAAATTGAGGAACCACAAAATTCGAACCTGCAGTGACTGTGATGTTATCTCACATTTGGTATTAATATAATATATGATTGTTGGAATAATAATATGATTTAAATTTCCATGAGTAGTAACTCATTGTGTGATTTAAGGGGATGAGATTAATTATGATCTTTATCATCTGTGTGTCTCTATATGTAGTATGAAGAATATGGAGAGAATTTTGTGGACATGCTCGACGGAATGTTCTCTTTCGTGCTATTGGACACACGTGACAACAGTTTCATTGCTGCTCGCGATGCAATTGGGGTTACTTCCCTTTACATTGGTTGGGGACGTGATGGTAATTGAAATAATTCTAAATCACATTTATACATGTATGTGTGTTTTTTTTAATCTATAGCTCCATAGACGTTATCTAATTAAATAGTCTAATTTAAATTATGTTAGGCTCAGTTTGGATATCTTCGGAACTCAAAGGTCTGAATGATGATTGCGAACATTTTGAGACCTTTCCACCTGGTCACTTGTACTCTAGCAAGGAAGCTGGATTCAAAAGATGGTATAATCCAATTTGGTTCTCTGAGGATATTCCATCGACCCCGTATGATCCACTTGTTCTGAGACGCGCATTGGAGAATGTATGAATTAGTGTATTAGATTTGAAGATAAAGGCCTTCTTTGATAACCATTTTGTTTTAATTCCTATTTCTTATAATGATTTGTATTTTTAAACTCAAAATTTTGAAAAACAAAATAGTTATCAAACGAGATCTAAGTTTATCGTCTTTTGAAATTCTATTAGGATAACATGGTTACATTGTTAACTTATGCAGGCTGTGATTAAGAGGCTGATGACGGACGTCCCTTTTGGAGTTCTGTTATCTGGTGGCCTAGATTCATCATTGGTTGCATCTATCACAGCTCGTTATTTAACTGGCACAAGAGCTGCTAAGCATTGGGGTACACAACTTCATTCCTTCTGTGTTGGCCTTGAGGTTGGTTTAGTTTTCATTTTCTTTTTTATGTATTGTATAATATAACGTTAATAATAGGTGATTTAAAATGATATTAAAGCATGTGCTCCAAGAGCTACTGTGTTCAATCCTTACAATGTTATTTTCCTTCATCCATCACCTTGTCAATTGGTAATTTAAGATAATACTTCACTATTCATCAACTGTGTACACTACACGCTTGTCCATTTCTCATTGGAGAAAAAGATTCATCCTTATGATGTTCTTCAGGGTTCACCAGATTTGAAGGCTGCTAGAGAAGTTGCAGACTTTTTGGGTACTGTCCACCATGAGTTTCATTTCACAGTTCAGGTACAACTTATATATCTACAACTTTACAAATCACAAATGTGCATTAACTTCCATTTTTATGATCTAGTTTATTTACCATCTCAATTCTCTTCTTTGATGATACAAGGATGGGATTGATGCCATTGAAGATGTTATCTACCATATAGAAACATACGATGTAACTACAATCAGGGCAAGTACACCCATGTTTCTGATGGCACGAAAAATTAAGTCACTAGGAGTAAAGATGGTGGTCTCTGGTGAAGGTGCTGATGAGATTTTTGGTGGATACCTCTACTTTCATAAGGCTCCTAATAAGGAAGAGTTTCACCGTGAAAGTTGTCGCAAGGTATACGACTTTAAGCACGTTAAGTGATATAATATTAAATTTGTCTTCTTATCCATAAGCTCAAGCTTTTGTTTCTCTCTTTTCAGATAAAGGCTCTTCACATGTATGATTGCTTGAGAGCAAACAAGGCGACTTCAGCATGGGGCTTGGAAGTTAGAGTTCCATTTTTAGATAAGAAATTTATCGATGTTGCAATGGCCATTGATCCAGAATGGAAAATGGTTAGAGAAAAAACTACTTCAAACTAGCCATATCATGATTAGCACTCTTGGTTTTTCCCCAAAATCTTCTTAACTCTCACCTAATATTTTATTCAATTTCAGATCAAACATAATCAAGGTCGAATTGAGAAATGGGTTCTCAGAAGAGCTTTCGACGACGAACAACAACCTTATCTACCTAAGGTTTTTGACCAAAAAAAAAACCCTCTCTAATTGTATACAAAAACTATAACTTCGATGGTAAAGTAACCTGTTTTCCTATATGCAGAATGTTTTGTACAGGCAAAAAGAACAATTCAGTGATGGTGTTGGATATAGTTGGATCGATGGCCTTAAAGCCCATGCTGATCAACATGTAATTCGATTAAACTTCCTCCCTATTGTCTTACAAGCGATGTTTGAGATGAGAAGTGGAATAGTGAGAAGTAAAGACTCATGAAGTCTAGGAAGTTGTGGATTTTCAGATTCTATAAGTAGTTGATAAGATATTGATGATTTTTAGCCGTGGTGGAACGTCCCATTAGCTTCTTAGGCAAAACAAGTAGTGGTGTCCACAACTACTTTTCAACTTTTACTTCACAAGTCCTTACGTGAAACACACTATGTTGGAAAGTAAAGAGTTATCGACTCACTCCTTATTTGGTTCAAGGAGTTTGTAAGTCACACCAAATAATCAATTTTATAACTTCTTATATTGTTGAGTTAAGTTCTAGGAGTTCACAAGTCTCTAGGCTAATTCTTTACTTCTCACTATTCTACCTCGTACCTCAAACACCGTCTCTGTGTTTTTATTGAAGAATGAAGTTTGGAGATCTAATATTTTTCAAGTAATGTTCACATGCTTTGACTAATTACTCAATACATGGTTTCAGGTGACTGATAAAATGATTTTGAATGCTGAACACATCTTCCCTCAAAATACTCCAACATCAAAGGAAGCCTACTACTACAGAACCATATTTGAGAGGTTCTTCCCACAGGTAATGTACTTCCATCAACAACAACAATGTTAACCACACGTCTCAGTGAACTTCAATTCACTAATCTCGTTTTACACTTTATATGGCTGTGCAGAACTCAGCTTGTCTGACTGTCCCGGGAGGACCAAGTATTGCTTGCAGTACGGCGAAGGCCGTCGAATGGGACGCCGCTTGGTCTAAAAATCTTGATCCTTCAGGTAGAGCCGCGCTCGGAGTTCATGCCTCAACATATGGAGACGAGGCAAATGGTCTAAATGACATCGTCCCACCTAAAATAGTTGATACCATTCCGAGAATGGAGGTCAGTACAGCAGGAGTAAAAATCCTTAGCTAG

mRNA sequence

ATGCTTGGCCTTGTCTTTCCAACGATTTTACGTCTTAATCTCTGCGATTTGTTTTCCATTTTCCTTATTAATTCTCACTTATTTTCCTCTCCTATTTTTTCTCTTTCAATCAGATTTCCTCTGTTTTTTTTGCCTTTTGCTTTGCTCAATATGTGTGGGATTCTAGCCGTTCTTGGTTGCTCCGATGATTCTCAGGCCAAACGTGTTCGTGTTCTTGAACTCTCTCGTCGATTGAAACACCGAGGCCCAGACTGGAGTGGGCTTTACCAACACGCTGATTGCTATTTGTCCCATCAACGTTTAGCCATTGTCGATCCTGCATCCGGTGATCAACCTCTCTATAATGAAAATAAATCCATCGTTGTCACGGTCAATGGAGAAATATACAACCACGAGGAGCTGAGGAAGAAATTGAGGAACCACAAAATTCGAACCTGCAGTGACTGTGATGTTATCTCACATTTGTATGAAGAATATGGAGAGAATTTTGTGGACATGCTCGACGGAATGTTCTCTTTCGTGCTATTGGACACACGTGACAACAGTTTCATTGCTGCTCGCGATGCAATTGGGGTTACTTCCCTTTACATTGGTTGGGGACGTGATGGCTCAGTTTGGATATCTTCGGAACTCAAAGGTCTGAATGATGATTGCGAACATTTTGAGACCTTTCCACCTGGTCACTTGTACTCTAGCAAGGAAGCTGGATTCAAAAGATGGTATAATCCAATTTGGTTCTCTGAGGATATTCCATCGACCCCGTATGATCCACTTGTTCTGAGACGCGCATTGGAGAATGCTGTGATTAAGAGGCTGATGACGGACGTCCCTTTTGGAGTTCTGTTATCTGGTGGCCTAGATTCATCATTGGTTGCATCTATCACAGCTCGTTATTTAACTGGCACAAGAGCTGCTAAGCATTGGGGTACACAACTTCATTCCTTCTGTGTTGGCCTTGAGGGTTCACCAGATTTGAAGGCTGCTAGAGAAGTTGCAGACTTTTTGGGTACTGTCCACCATGAGTTTCATTTCACAGTTCAGGATGGGATTGATGCCATTGAAGATGTTATCTACCATATAGAAACATACGATGTAACTACAATCAGGGCAAGTACACCCATGTTTCTGATGGCACGAAAAATTAAGTCACTAGGAGTAAAGATGGTGGTCTCTGGTGAAGGTGCTGATGAGATTTTTGGTGGATACCTCTACTTTCATAAGGCTCCTAATAAGGAAGAGTTTCACCGTGAAAGTTGTCGCAAGATAAAGGCTCTTCACATGTATGATTGCTTGAGAGCAAACAAGGCGACTTCAGCATGGGGCTTGGAAGTTAGAGTTCCATTTTTAGATAAGAAATTTATCGATGTTGCAATGGCCATTGATCCAGAATGGAAAATGATCAAACATAATCAAGGTCGAATTGAGAAATGGGTTCTCAGAAGAGCTTTCGACGACGAACAACAACCTTATCTACCTAAGAATGTTTTGTACAGGCAAAAAGAACAATTCAGTGATGGTGTTGGATATAGTTGGATCGATGGCCTTAAAGCCCATGCTGATCAACATGTGACTGATAAAATGATTTTGAATGCTGAACACATCTTCCCTCAAAATACTCCAACATCAAAGGAAGCCTACTACTACAGAACCATATTTGAGAGGTTCTTCCCACAGAACTCAGCTTGTCTGACTGTCCCGGGAGGACCAAGTATTGCTTGCAGTACGGCGAAGGCCGTCGAATGGGACGCCGCTTGGTCTAAAAATCTTGATCCTTCAGGTAGAGCCGCGCTCGGAGTTCATGCCTCAACATATGGAGACGAGGCAAATGGTCTAAATGACATCGTCCCACCTAAAATAGTTGATACCATTCCGAGAATGGAGGTCAGTACAGCAGGAGTAAAAATCCTTAGCTAG

Coding sequence (CDS)

ATGCTTGGCCTTGTCTTTCCAACGATTTTACGTCTTAATCTCTGCGATTTGTTTTCCATTTTCCTTATTAATTCTCACTTATTTTCCTCTCCTATTTTTTCTCTTTCAATCAGATTTCCTCTGTTTTTTTTGCCTTTTGCTTTGCTCAATATGTGTGGGATTCTAGCCGTTCTTGGTTGCTCCGATGATTCTCAGGCCAAACGTGTTCGTGTTCTTGAACTCTCTCGTCGATTGAAACACCGAGGCCCAGACTGGAGTGGGCTTTACCAACACGCTGATTGCTATTTGTCCCATCAACGTTTAGCCATTGTCGATCCTGCATCCGGTGATCAACCTCTCTATAATGAAAATAAATCCATCGTTGTCACGGTCAATGGAGAAATATACAACCACGAGGAGCTGAGGAAGAAATTGAGGAACCACAAAATTCGAACCTGCAGTGACTGTGATGTTATCTCACATTTGTATGAAGAATATGGAGAGAATTTTGTGGACATGCTCGACGGAATGTTCTCTTTCGTGCTATTGGACACACGTGACAACAGTTTCATTGCTGCTCGCGATGCAATTGGGGTTACTTCCCTTTACATTGGTTGGGGACGTGATGGCTCAGTTTGGATATCTTCGGAACTCAAAGGTCTGAATGATGATTGCGAACATTTTGAGACCTTTCCACCTGGTCACTTGTACTCTAGCAAGGAAGCTGGATTCAAAAGATGGTATAATCCAATTTGGTTCTCTGAGGATATTCCATCGACCCCGTATGATCCACTTGTTCTGAGACGCGCATTGGAGAATGCTGTGATTAAGAGGCTGATGACGGACGTCCCTTTTGGAGTTCTGTTATCTGGTGGCCTAGATTCATCATTGGTTGCATCTATCACAGCTCGTTATTTAACTGGCACAAGAGCTGCTAAGCATTGGGGTACACAACTTCATTCCTTCTGTGTTGGCCTTGAGGGTTCACCAGATTTGAAGGCTGCTAGAGAAGTTGCAGACTTTTTGGGTACTGTCCACCATGAGTTTCATTTCACAGTTCAGGATGGGATTGATGCCATTGAAGATGTTATCTACCATATAGAAACATACGATGTAACTACAATCAGGGCAAGTACACCCATGTTTCTGATGGCACGAAAAATTAAGTCACTAGGAGTAAAGATGGTGGTCTCTGGTGAAGGTGCTGATGAGATTTTTGGTGGATACCTCTACTTTCATAAGGCTCCTAATAAGGAAGAGTTTCACCGTGAAAGTTGTCGCAAGATAAAGGCTCTTCACATGTATGATTGCTTGAGAGCAAACAAGGCGACTTCAGCATGGGGCTTGGAAGTTAGAGTTCCATTTTTAGATAAGAAATTTATCGATGTTGCAATGGCCATTGATCCAGAATGGAAAATGATCAAACATAATCAAGGTCGAATTGAGAAATGGGTTCTCAGAAGAGCTTTCGACGACGAACAACAACCTTATCTACCTAAGAATGTTTTGTACAGGCAAAAAGAACAATTCAGTGATGGTGTTGGATATAGTTGGATCGATGGCCTTAAAGCCCATGCTGATCAACATGTGACTGATAAAATGATTTTGAATGCTGAACACATCTTCCCTCAAAATACTCCAACATCAAAGGAAGCCTACTACTACAGAACCATATTTGAGAGGTTCTTCCCACAGAACTCAGCTTGTCTGACTGTCCCGGGAGGACCAAGTATTGCTTGCAGTACGGCGAAGGCCGTCGAATGGGACGCCGCTTGGTCTAAAAATCTTGATCCTTCAGGTAGAGCCGCGCTCGGAGTTCATGCCTCAACATATGGAGACGAGGCAAATGGTCTAAATGACATCGTCCCACCTAAAATAGTTGATACCATTCCGAGAATGGAGGTCAGTACAGCAGGAGTAAAAATCCTTAGCTAG

Protein sequence

MLGLVFPTILRLNLCDLFSIFLINSHLFSSPIFSLSIRFPLFFLPFALLNMCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGDQPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGMFSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLYSSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHNQGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS*
Homology
BLAST of CsaV3_6G031950 vs. NCBI nr
Match: KAE8647225.1 (hypothetical protein Csa_018945 [Cucumis sativus])

HSP 1 Score: 1313.9 bits (3399), Expect = 0.0e+00
Identity = 637/637 (100.00%), Postives = 637/637 (100.00%), Query Frame = 0

Query: 1   MLGLVFPTILRLNLCDLFSIFLINSHLFSSPIFSLSIRFPLFFLPFALLNMCGILAVLGC 60
           MLGLVFPTILRLNLCDLFSIFLINSHLFSSPIFSLSIRFPLFFLPFALLNMCGILAVLGC
Sbjct: 1   MLGLVFPTILRLNLCDLFSIFLINSHLFSSPIFSLSIRFPLFFLPFALLNMCGILAVLGC 60

Query: 61  SDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGDQPLYNENKSI 120
           SDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGDQPLYNENKSI
Sbjct: 61  SDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGDQPLYNENKSI 120

Query: 121 VVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGMFSFVLLDTRD 180
           VVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGMFSFVLLDTRD
Sbjct: 121 VVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGMFSFVLLDTRD 180

Query: 181 NSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLYSSKEAGFKRW 240
           NSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLYSSKEAGFKRW
Sbjct: 181 NSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLYSSKEAGFKRW 240

Query: 241 YNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLT 300
           YNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLT
Sbjct: 241 YNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLT 300

Query: 301 GTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHI 360
           GTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHI
Sbjct: 301 GTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHI 360

Query: 361 ETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPNKEEFHRESCR 420
           ETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPNKEEFHRESCR
Sbjct: 361 ETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPNKEEFHRESCR 420

Query: 421 KIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHNQGRIEKWVLR 480
           KIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHNQGRIEKWVLR
Sbjct: 421 KIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHNQGRIEKWVLR 480

Query: 481 RAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILNAEHIFPQNTP 540
           RAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILNAEHIFPQNTP
Sbjct: 481 RAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILNAEHIFPQNTP 540

Query: 541 TSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVH 600
           TSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVH
Sbjct: 541 TSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVH 600

Query: 601 ASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS 638
           ASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS
Sbjct: 601 ASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS 637

BLAST of CsaV3_6G031950 vs. NCBI nr
Match: XP_004144212.3 (asparagine synthetase [glutamine-hydrolyzing] isoform X1 [Cucumis sativus])

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 587/587 (100.00%), Postives = 587/587 (100.00%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM
Sbjct: 61  QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN
Sbjct: 361 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
           QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD
Sbjct: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 540

Query: 591 PSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS 638
           PSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS
Sbjct: 541 PSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS 587

BLAST of CsaV3_6G031950 vs. NCBI nr
Match: TYK00726.1 (asparagine synthetase [Cucumis melo var. makuwa])

HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 574/614 (93.49%), Postives = 580/614 (94.46%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRR--LKHRGPDWSGLYQHADCYLSHQRLAIVDPAS 110
           MCGILAVLGCSDDSQAKRVRVLELSRR  LKHRGPDWSGLYQH DCYLSHQRLAIVDPAS
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHKDCYLSHQRLAIVDPAS 60

Query: 111 GDQPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLD 170
           GDQPLYNENKSIVVTVNGEIYNHEELRKKL+NHKIRT SDCDVISHLYEEYGENFVDMLD
Sbjct: 61  GDQPLYNENKSIVVTVNGEIYNHEELRKKLKNHKIRTGSDCDVISHLYEEYGENFVDMLD 120

Query: 171 GMFSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGH 230
           GMFSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGH
Sbjct: 121 GMFSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGH 180

Query: 231 LYSSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDS 290
           LYSSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDS
Sbjct: 181 LYSSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDS 240

Query: 291 SLVASITARYLTGTRAAKHWGTQLHSFCVGLE--------------------------GS 350
           SLVASITAR+LTGTRAAKHWGTQLHSFCVGLE                          GS
Sbjct: 241 SLVASITARHLTGTRAAKHWGTQLHSFCVGLEHVFQELLFNPCNVISLHPSSFIHQFKGS 300

Query: 351 PDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARKIK 410
           PDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARKIK
Sbjct: 301 PDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARKIK 360

Query: 411 SLGVKMVVSGEGADEIFGGYLYFHKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGL 470
           SLGVKMV+SGEGADEIFGGYLYFHKAPNKEEFHRESC KIKALHMYDCLRANKATSAWGL
Sbjct: 361 SLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHRESCHKIKALHMYDCLRANKATSAWGL 420

Query: 471 EVRVPFLDKKFIDVAMAIDPEWKMIKHNQGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQ 530
           E+RVPFLDKKFI+VAMAIDPEWKMIKH+QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQ
Sbjct: 421 EIRVPFLDKKFIEVAMAIDPEWKMIKHDQGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQ 480

Query: 531 FSDGVGYSWIDGLKAHADQHVTDKMILNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSAC 590
           FSDGVGYSWIDGLKAHADQHVTDKMILNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSAC
Sbjct: 481 FSDGVGYSWIDGLKAHADQHVTDKMILNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSAC 540

Query: 591 LTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASTYGDEANGLNDIVPPKIVDT 637
           LTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASTYGDEANGLN IV PKIVDT
Sbjct: 541 LTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASTYGDEANGLNAIVQPKIVDT 600

BLAST of CsaV3_6G031950 vs. NCBI nr
Match: XP_004143163.1 (asparagine synthetase [glutamine-hydrolyzing] [Cucumis sativus] >KGN47067.1 hypothetical protein Csa_020758 [Cucumis sativus])

HSP 1 Score: 1163.3 bits (3008), Expect = 0.0e+00
Identity = 552/587 (94.04%), Postives = 573/587 (97.61%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQH DCYL+HQRLAI+DPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHKDCYLAHQRLAIIDPASGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPLYNE++S+VVTVNGEIYNHEELRKKLRNHKIRT SDCDVISHLYEEYGENFVDMLDGM
Sbjct: 61  QPLYNEDQSVVVTVNGEIYNHEELRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLDGM 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           FSFVLLDTRDNSF+ ARDAIG+TSLYIGWG DGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SSKEA FKRWYNP WFSEDIPSTPYDPLVLRRA ENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRAFENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VASITAR+LTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIEDVIYHIETYDVTTIRASTPM+LMARKIKSLGVKMV+SGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDK+FI+VAMAIDPEWKMIK +
Sbjct: 361 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKRD 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
           QGRIEKWVLRRAFDDEQQPYLPK+VLYRQKEQFSDGVGYSWIDGLKAHA QHVTDKM+LN
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           AEHIFPQNTPTSKEAYYYRTIFERFFPQNSA LTVPGGPSIACSTAKAVEWDAAWSKNLD
Sbjct: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLD 540

Query: 591 PSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS 638
           PSGRAALGVHA+ YGDE NG+N++ PPKI+D+IPRMEVST GVKILS
Sbjct: 541 PSGRAALGVHAAAYGDETNGVNNVAPPKIIDSIPRMEVSTPGVKILS 587

BLAST of CsaV3_6G031950 vs. NCBI nr
Match: XP_008465262.1 (PREDICTED: asparagine synthetase [glutamine-hydrolyzing] 1-like [Cucumis melo])

HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 555/587 (94.55%), Postives = 573/587 (97.61%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQH DCYL+HQRLAI+DPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHKDCYLAHQRLAIIDPASGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPLYNE+KSIVVTVNGEIYNHEELRKKLRNHKIRT SDCDVISHLYEEYGENFVDMLDGM
Sbjct: 61  QPLYNEDKSIVVTVNGEIYNHEELRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLDGM 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           FSFVLLDTRDNSF+ ARDAIG+TSLYIGWG DGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SSKEA FKRWYNP WFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VASITAR+LTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIEDVIYHIETYDVTTIRASTPM+LMARKIKSLGVKMV+SGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDK+FI+VAMAIDPEWKMIK +
Sbjct: 361 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKRD 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
           QGRIEKWVLRRAFDDEQQPYLPK+VLYRQKEQFSDGVGYSWIDGLKAHA QHVTDKM+LN
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           AEHIFPQNTPTSKEAYYYRTIFERFFPQNSA LTVPGGPSIACSTAKA+EWDAAWSKNLD
Sbjct: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAWSKNLD 540

Query: 591 PSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS 638
           PSGRAALGVHA+ YGDEANG+N I PPKI+D+IPRMEV T GVKILS
Sbjct: 541 PSGRAALGVHAAAYGDEANGVN-IAPPKIIDSIPRMEVGTPGVKILS 586

BLAST of CsaV3_6G031950 vs. ExPASy Swiss-Prot
Match: P49092 (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus OX=34305 GN=AS1 PE=2 SV=2)

HSP 1 Score: 1013.4 bits (2619), Expect = 1.1e-294
Identity = 480/587 (81.77%), Postives = 531/587 (90.46%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGCSD +QAKRVRVLELSRRLKHRGPDWSGL+QH DCYL+HQRLAIVDPASGD
Sbjct: 1   MCGILAVLGCSDFTQAKRVRVLELSRRLKHRGPDWSGLHQHGDCYLAHQRLAIVDPASGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPL+NE+KSI+VTVNGEIYNHEELRK+L NH+ RT SDCDVI+HLYEE+GENF+DMLDG+
Sbjct: 61  QPLFNEDKSIIVTVNGEIYNHEELRKQLPNHQFRTGSDCDVIAHLYEEHGENFMDMLDGI 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           FSFVLLDTRDN+FI ARDAIGVTSLYIGWG DGSVWISSE+KGLNDDCEHFE FPPGHLY
Sbjct: 121 FSFVLLDTRDNTFIVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFEVFPPGHLY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SS+E  F+RWYNP WFSE IPS PYDPL +R A E AVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSRERAFRRWYNPTWFSESIPSAPYDPLAVRHAFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VASIT+RYL  T+AA+ WG++LHSFCVGLEGSPDLKAA+EVAD+LGTVHHEF FTVQDGI
Sbjct: 241 VASITSRYLATTKAAEQWGSKLHSFCVGLEGSPDLKAAKEVADYLGTVHHEFTFTVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIE+VIYH+ETYDVTTIRASTPMFLM+RKIKSLGVK V+SGEG+DEIFGGYLYFHKAPN
Sbjct: 301 DAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKWVISGEGSDEIFGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           KEEFH E+CRKIKALH YDCLRANK+T AWGLE RVPFLDK+FI+VAM IDPE+KMIK +
Sbjct: 361 KEEFHTETCRKIKALHQYDCLRANKSTFAWGLEARVPFLDKEFINVAMNIDPEYKMIKRD 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
           +GRIEK++LRRAFDDE++PYLPK++LYRQKEQFSDGVGYSWIDGLK HA +HVTDKMILN
Sbjct: 421 EGRIEKYILRRAFDDEEKPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAKHVTDKMILN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           A +IF  NTP +KEAYYYR IFERFFPQNSA LTVPGGP++ACSTAKAVEWDAAWS NLD
Sbjct: 481 AGNIFRHNTPLTKEAYYYRMIFERFFPQNSARLTVPGGPTVACSTAKAVEWDAAWSNNLD 540

Query: 591 PSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS 638
           PSGRAALGVH S Y D+ N L +  P +    IP ME  + GV I S
Sbjct: 541 PSGRAALGVHLSAYDDKQNNLINNKPVEFEKLIP-MEAPSLGVAIHS 586

BLAST of CsaV3_6G031950 vs. ExPASy Swiss-Prot
Match: O24661 (Asparagine synthetase [glutamine-hydrolyzing] OS=Triphysaria versicolor OX=64093 GN=AS PE=2 SV=3)

HSP 1 Score: 1011.5 bits (2614), Expect = 4.1e-294
Identity = 473/554 (85.38%), Postives = 516/554 (93.14%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSG++ H DCYL+HQRLAIVDPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGIHHHGDCYLAHQRLAIVDPASGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPL+NE+K I VTVNGEIYNHEELR  L NHK RT SDCDVI+HLYEEYGENFV+MLDGM
Sbjct: 61  QPLFNEDKRIAVTVNGEIYNHEELRALLPNHKFRTGSDCDVIAHLYEEYGENFVEMLDGM 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           FSFVLLD+RDN+FIAARDA G+TSLYIGWG DGSVWISSELKGL+D+CE+FE FPPGH+Y
Sbjct: 121 FSFVLLDSRDNTFIAARDAFGITSLYIGWGLDGSVWISSELKGLHDECENFEVFPPGHVY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SSK  GF+RWYNP WFSE IPSTPYDPLVLR A E AVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKTEGFRRWYNPPWFSEAIPSTPYDPLVLRGAFEQAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VA++TAR+L GT+AAK WG+QLHSFCVGLEGSPDLKA +EVAD+LGTVHHEF FTVQDGI
Sbjct: 241 VAAVTARHLAGTKAAKRWGSQLHSFCVGLEGSPDLKAGKEVADYLGTVHHEFLFTVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIEDVIYHIETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEG+DEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           KEEFHRE+CRKIKALH YDCLRANKATSAWGLE RVPFLDK+F+++AM+IDPE KMIK +
Sbjct: 361 KEEFHRETCRKIKALHQYDCLRANKATSAWGLEARVPFLDKEFVNLAMSIDPEAKMIKPD 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
           QGRIEKW+LR+AFDDE++PYLPK++LYRQKEQFSDGVGYSWIDGLKAHA+QHVTDKM+LN
Sbjct: 421 QGRIEKWILRKAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAEQHVTDKMMLN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           A HIFP NTPT+KE YYYR IFERFFPQNSA LTVPGGPS+ACSTA AV WDA+WSKNLD
Sbjct: 481 AGHIFPHNTPTTKEGYYYRMIFERFFPQNSAKLTVPGGPSVACSTATAVAWDASWSKNLD 540

Query: 591 PSGRAALGVHASTY 605
           PSGRAA GVH   Y
Sbjct: 541 PSGRAATGVHDLAY 554

BLAST of CsaV3_6G031950 vs. ExPASy Swiss-Prot
Match: P31752 (Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis OX=4686 PE=2 SV=2)

HSP 1 Score: 1007.7 bits (2604), Expect = 6.0e-293
Identity = 468/554 (84.48%), Postives = 518/554 (93.50%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGL QH DC+LSHQRLAI+DPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLCQHGDCFLSHQRLAIIDPASGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPLYNE+KSIVVTVNGEIYNHEELR++L +HK RT SDC+VI+HLYEE+GE+FVDMLDGM
Sbjct: 61  QPLYNEDKSIVVTVNGEIYNHEELRRRLPDHKYRTGSDCEVIAHLYEEHGEDFVDMLDGM 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           FSFVLLDTR+N F+AARDA+G+T LYIGWG DGSVW+SSE+KGLNDDCEHFE FPPG+LY
Sbjct: 121 FSFVLLDTRNNCFVAARDAVGITPLYIGWGLDGSVWLSSEMKGLNDDCEHFEVFPPGNLY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SS+   F+RWYNP W++E IPS PYDPLVLR+A E+AVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSRSGSFRRWYNPQWYNETIPSAPYDPLVLRKAFEDAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VA++TAR+L G++AA+ WGTQLHSFCVGLEGSPDLKAA+EVA++LGTVHHEFHFTVQDGI
Sbjct: 241 VAAVTARHLAGSKAAEQWGTQLHSFCVGLEGSPDLKAAKEVAEYLGTVHHEFHFTVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIEDVI+HIETYDVTTIRASTPMFLMARKIKSLGVKMV+SGEG+DEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIFHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGSDEIFGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           KEEFH E+CRKIKALH YDCLRANKATSAWGLE RVPFLDK+F+DVAM+IDPE KMIK +
Sbjct: 361 KEEFHHETCRKIKALHQYDCLRANKATSAWGLEARVPFLDKEFMDVAMSIDPESKMIKPD 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
            GRIEKWVLR+AFDDE+ PYLPK++LYRQKEQFSDGVGYSWIDGLKAHA +HVTD+M+LN
Sbjct: 421 LGRIEKWVLRKAFDDEENPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAAKHVTDRMMLN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           A  I+P NTPT+KEAYYYR IFERFFPQNSA  TVPGGPSIACSTAKA+EWDA WS NLD
Sbjct: 481 AARIYPHNTPTTKEAYYYRMIFERFFPQNSARFTVPGGPSIACSTAKAIEWDARWSNNLD 540

Query: 591 PSGRAALGVHASTY 605
           PSGRAALGVH S Y
Sbjct: 541 PSGRAALGVHDSAY 554

BLAST of CsaV3_6G031950 vs. ExPASy Swiss-Prot
Match: P49078 (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Arabidopsis thaliana OX=3702 GN=ASN1 PE=2 SV=2)

HSP 1 Score: 1007.3 bits (2603), Expect = 7.8e-293
Identity = 473/576 (82.12%), Postives = 526/576 (91.32%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGCSDDSQAKRVRVLELSRRL+HRGPDWSGLYQ+ D YL+HQRLA++DPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGDNYLAHQRLAVIDPASGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPL+NE+K+IVVTVNGEIYNHEELRK+L+NHK RT SDC+VI+HLYEEYG +FVDMLDG+
Sbjct: 61  QPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVIAHLYEEYGVDFVDMLDGI 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           FSFVLLDTRDNSF+ ARDAIGVTSLYIGWG DGSVWISSE+KGLNDDCEHFETFPPGH Y
Sbjct: 121 FSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFETFPPGHFY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SSK  GFK+WYNP WF+E +PSTPY+PL +RRA ENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKLGGFKQWYNPPWFNESVPSTPYEPLAIRRAFENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VASITAR+L GT+AAK WG QLHSFCVGLEGSPDLKA +EVA++LGTVHHEFHF+VQDGI
Sbjct: 241 VASITARHLAGTKAAKQWGPQLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHFSVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIEDVIYH+ETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGADEIFGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           K+EFH+E+CRKIKALH YDCLRANK+TSA+GLE RVPFLDK FI+ AM++DPE KMIK  
Sbjct: 361 KKEFHQETCRKIKALHKYDCLRANKSTSAFGLEARVPFLDKDFINTAMSLDPESKMIKPE 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
           +GRIEKWVLRRAFDDE++PYLPK++LYRQKEQFSDGVGYSWIDGLK HA Q+V DKM+ N
Sbjct: 421 EGRIEKWVLRRAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAQNVNDKMMSN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           A HIFP NTP +KEAYYYR IFERFFPQNSA LTVPGG ++ACSTAKAVEWDA+WS N+D
Sbjct: 481 AGHIFPHNTPNTKEAYYYRMIFERFFPQNSARLTVPGGATVACSTAKAVEWDASWSNNMD 540

Query: 591 PSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPRM 627
           PSGRAA+GVH S Y D  N    I P K +D +P M
Sbjct: 541 PSGRAAIGVHLSAY-DGKNVALTIPPLKAIDNMPMM 575

BLAST of CsaV3_6G031950 vs. ExPASy Swiss-Prot
Match: P49093 (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Lotus japonicus OX=34305 GN=AS2 PE=2 SV=2)

HSP 1 Score: 1005.4 bits (2598), Expect = 3.0e-292
Identity = 478/589 (81.15%), Postives = 528/589 (89.64%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGL+QH D +L+HQRLAIVDPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLHQHGDNFLAHQRLAIVDPASGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPL+NE++SI+VTVNGEI+NHEELRK+L NHK RT  DCDVI+HLYEE+GENFVDMLDG+
Sbjct: 61  QPLFNEDQSIIVTVNGEIFNHEELRKQLPNHKFRTGCDCDVIAHLYEEHGENFVDMLDGI 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           FSFVLLDTRDNSF+ ARDAIGVTSLYIG+G DGSVWI+SELKGLNDDCEHFE FPPGHLY
Sbjct: 121 FSFVLLDTRDNSFLVARDAIGVTSLYIGYGLDGSVWIASELKGLNDDCEHFELFPPGHLY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SSKE  F+RWYNP WFSE IPS PYDPL LR+A E A+IKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEKEFRRWYNPPWFSEAIPSAPYDPLALRQAFEKAIIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VAS+TARYL  T+AAK WG++LHSFCVGLEG+PDLKAAREVAD++GTVHHEF +T+QDGI
Sbjct: 241 VASVTARYLADTKAAKQWGSKLHSFCVGLEGAPDLKAAREVADYIGTVHHEFQYTIQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIEDVIYH+ETYDVTTIRA TPMFLM+RKIKSLGVKMV+SGEG+DEIF GYLYFHKAPN
Sbjct: 301 DAIEDVIYHVETYDVTTIRAGTPMFLMSRKIKSLGVKMVLSGEGSDEIFAGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           KEE H+E+C KIKALH YDCLRANK+T AWGLE RVPFLDKKFIDVAM IDPE KMIK +
Sbjct: 361 KEELHQETCSKIKALHKYDCLRANKSTYAWGLEARVPFLDKKFIDVAMGIDPENKMIKRD 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
           +GRIEKWVLR+AFDDE+ PYLPK++LYRQKEQFSDGVGY WIDGLK HA +HVTDKM+LN
Sbjct: 421 EGRIEKWVLRKAFDDEENPYLPKHILYRQKEQFSDGVGYGWIDGLKDHAAKHVTDKMMLN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           A +I+P NTP +KEAYYYR IFERFFPQNSA L+VPGG SIACST KA+EWDAAWS NLD
Sbjct: 481 ASNIYPFNTPNTKEAYYYRMIFERFFPQNSARLSVPGGASIACSTEKAIEWDAAWSNNLD 540

Query: 591 PSGRAALGVHASTYGDEANG--LNDIVPPKIVDTIPRMEVSTAGVKILS 638
           PSGRAALGVH S Y D+ N      + P KI   IP+MEVS  GV ILS
Sbjct: 541 PSGRAALGVHDSAYDDQLNKSVSKGVEPEKI---IPKMEVSPLGVAILS 586

BLAST of CsaV3_6G031950 vs. ExPASy TrEMBL
Match: A0A5D3BR39 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold187G00140 PE=4 SV=1)

HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 574/614 (93.49%), Postives = 580/614 (94.46%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRR--LKHRGPDWSGLYQHADCYLSHQRLAIVDPAS 110
           MCGILAVLGCSDDSQAKRVRVLELSRR  LKHRGPDWSGLYQH DCYLSHQRLAIVDPAS
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHKDCYLSHQRLAIVDPAS 60

Query: 111 GDQPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLD 170
           GDQPLYNENKSIVVTVNGEIYNHEELRKKL+NHKIRT SDCDVISHLYEEYGENFVDMLD
Sbjct: 61  GDQPLYNENKSIVVTVNGEIYNHEELRKKLKNHKIRTGSDCDVISHLYEEYGENFVDMLD 120

Query: 171 GMFSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGH 230
           GMFSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGH
Sbjct: 121 GMFSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGH 180

Query: 231 LYSSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDS 290
           LYSSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDS
Sbjct: 181 LYSSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDS 240

Query: 291 SLVASITARYLTGTRAAKHWGTQLHSFCVGLE--------------------------GS 350
           SLVASITAR+LTGTRAAKHWGTQLHSFCVGLE                          GS
Sbjct: 241 SLVASITARHLTGTRAAKHWGTQLHSFCVGLEHVFQELLFNPCNVISLHPSSFIHQFKGS 300

Query: 351 PDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARKIK 410
           PDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARKIK
Sbjct: 301 PDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARKIK 360

Query: 411 SLGVKMVVSGEGADEIFGGYLYFHKAPNKEEFHRESCRKIKALHMYDCLRANKATSAWGL 470
           SLGVKMV+SGEGADEIFGGYLYFHKAPNKEEFHRESC KIKALHMYDCLRANKATSAWGL
Sbjct: 361 SLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHRESCHKIKALHMYDCLRANKATSAWGL 420

Query: 471 EVRVPFLDKKFIDVAMAIDPEWKMIKHNQGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQ 530
           E+RVPFLDKKFI+VAMAIDPEWKMIKH+QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQ
Sbjct: 421 EIRVPFLDKKFIEVAMAIDPEWKMIKHDQGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQ 480

Query: 531 FSDGVGYSWIDGLKAHADQHVTDKMILNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSAC 590
           FSDGVGYSWIDGLKAHADQHVTDKMILNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSAC
Sbjct: 481 FSDGVGYSWIDGLKAHADQHVTDKMILNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSAC 540

Query: 591 LTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASTYGDEANGLNDIVPPKIVDT 637
           LTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASTYGDEANGLN IV PKIVDT
Sbjct: 541 LTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASTYGDEANGLNAIVQPKIVDT 600

BLAST of CsaV3_6G031950 vs. ExPASy TrEMBL
Match: A0A0A0KF94 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis sativus OX=3659 GN=Csa_6G183190 PE=4 SV=1)

HSP 1 Score: 1163.3 bits (3008), Expect = 0.0e+00
Identity = 552/587 (94.04%), Postives = 573/587 (97.61%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQH DCYL+HQRLAI+DPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHKDCYLAHQRLAIIDPASGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPLYNE++S+VVTVNGEIYNHEELRKKLRNHKIRT SDCDVISHLYEEYGENFVDMLDGM
Sbjct: 61  QPLYNEDQSVVVTVNGEIYNHEELRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLDGM 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           FSFVLLDTRDNSF+ ARDAIG+TSLYIGWG DGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SSKEA FKRWYNP WFSEDIPSTPYDPLVLRRA ENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRAFENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VASITAR+LTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIEDVIYHIETYDVTTIRASTPM+LMARKIKSLGVKMV+SGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDK+FI+VAMAIDPEWKMIK +
Sbjct: 361 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKRD 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
           QGRIEKWVLRRAFDDEQQPYLPK+VLYRQKEQFSDGVGYSWIDGLKAHA QHVTDKM+LN
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           AEHIFPQNTPTSKEAYYYRTIFERFFPQNSA LTVPGGPSIACSTAKAVEWDAAWSKNLD
Sbjct: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLD 540

Query: 591 PSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS 638
           PSGRAALGVHA+ YGDE NG+N++ PPKI+D+IPRMEVST GVKILS
Sbjct: 541 PSGRAALGVHAAAYGDETNGVNNVAPPKIIDSIPRMEVSTPGVKILS 587

BLAST of CsaV3_6G031950 vs. ExPASy TrEMBL
Match: A0A1S3CNG7 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo OX=3656 GN=LOC103502918 PE=4 SV=1)

HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 555/587 (94.55%), Postives = 573/587 (97.61%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQH DCYL+HQRLAI+DPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHKDCYLAHQRLAIIDPASGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPLYNE+KSIVVTVNGEIYNHEELRKKLRNHKIRT SDCDVISHLYEEYGENFVDMLDGM
Sbjct: 61  QPLYNEDKSIVVTVNGEIYNHEELRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLDGM 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           FSFVLLDTRDNSF+ ARDAIG+TSLYIGWG DGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SSKEA FKRWYNP WFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VASITAR+LTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIEDVIYHIETYDVTTIRASTPM+LMARKIKSLGVKMV+SGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDK+FI+VAMAIDPEWKMIK +
Sbjct: 361 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKRD 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
           QGRIEKWVLRRAFDDEQQPYLPK+VLYRQKEQFSDGVGYSWIDGLKAHA QHVTDKM+LN
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           AEHIFPQNTPTSKEAYYYRTIFERFFPQNSA LTVPGGPSIACSTAKA+EWDAAWSKNLD
Sbjct: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAWSKNLD 540

Query: 591 PSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS 638
           PSGRAALGVHA+ YGDEANG+N I PPKI+D+IPRMEV T GVKILS
Sbjct: 541 PSGRAALGVHAAAYGDEANGVN-IAPPKIIDSIPRMEVGTPGVKILS 586

BLAST of CsaV3_6G031950 vs. ExPASy TrEMBL
Match: A0A6J1J8M0 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita maxima OX=3661 GN=LOC111484448 PE=4 SV=1)

HSP 1 Score: 1138.6 bits (2944), Expect = 0.0e+00
Identity = 541/587 (92.16%), Postives = 563/587 (95.91%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGC+DDS AKRVRVLELSRRLKHRGPDWSGLYQH DCYL+HQRLAI+DPASGD
Sbjct: 1   MCGILAVLGCADDSLAKRVRVLELSRRLKHRGPDWSGLYQHGDCYLTHQRLAIIDPASGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPLYNE+KS+VVTVNGEIYNHEELRKKLRNHK RT SDCDVISHLYEEYGENFV MLDGM
Sbjct: 61  QPLYNEDKSVVVTVNGEIYNHEELRKKLRNHKFRTGSDCDVISHLYEEYGENFVGMLDGM 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           FSFVLLDTRDNSFIAARDAIG+TSLYIGWG DGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SSK+ GFKRWYNP WFSE IPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGFKRWYNPTWFSEGIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VASITAR+L GTRAAK WGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLAGTRAAKQWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMV+SGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           KEEFHRESCRKIK LH+YDCLRANKATSAWGLEVRVPFLDK+FI+VAMAIDPEWKM+K  
Sbjct: 361 KEEFHRESCRKIKELHLYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMVKRE 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
           QGRIEKWVLRRAFDDEQQPYLPK+VLYRQKEQFSDGVGYSWIDGLK+HA QHVTDKM+ N
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKSVLYRQKEQFSDGVGYSWIDGLKSHAAQHVTDKMMQN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           AEHIFPQNTPTSKEAYYYRTIFERFFPQNSA LTVPGGPSIACSTAKAVEWDAAWSKNLD
Sbjct: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLD 540

Query: 591 PSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS 638
           PSGRAALGVH S Y DEA  +N I+P K++DTIPRMEVST+G+KILS
Sbjct: 541 PSGRAALGVHVSAYADEATNVNSILPSKMIDTIPRMEVSTSGLKILS 587

BLAST of CsaV3_6G031950 vs. ExPASy TrEMBL
Match: A0A6J1G8T6 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita moschata OX=3662 GN=LOC111451773 PE=4 SV=1)

HSP 1 Score: 1137.9 bits (2942), Expect = 0.0e+00
Identity = 541/587 (92.16%), Postives = 561/587 (95.57%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQH D YL+HQRLAI+DPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHTDFYLTHQRLAIIDPASGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPL+NE+KSIVVTVNGEIYNHEELRKKL NHK RT SDCDVI+HLYEEYGENFVDMLDGM
Sbjct: 61  QPLFNEDKSIVVTVNGEIYNHEELRKKLSNHKFRTGSDCDVIAHLYEEYGENFVDMLDGM 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           FSFVLLDTRDNSFI ARDAIG+TSLYIGWG DGSVWISSELKGLNDDCEHFETFPPGH Y
Sbjct: 121 FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHFY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SSKE GFKRWYNP WFSE IPSTPYDPLVLRR+LENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEGGFKRWYNPTWFSEAIPSTPYDPLVLRRSLENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VASITAR+L GTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIEDVIYHIETYDVTTIRASTPMFLMARKIK+LGVKMV+SGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKALGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           KEEFHRESCRKIKALH+YDCLRANKATSAWGLEVRVPFLDK+FID AMAIDPEWKMIK  
Sbjct: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDTAMAIDPEWKMIKRE 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
           QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHA QHVTD+M+LN
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDRMMLN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           A HIFPQNTPTSKEAYYYRTIFERFFPQN+A LT+PGGPSIACSTAKAVEWDAAWSKNLD
Sbjct: 481 AGHIFPQNTPTSKEAYYYRTIFERFFPQNAAQLTIPGGPSIACSTAKAVEWDAAWSKNLD 540

Query: 591 PSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS 638
           PSGRAALGVH S YGDEA G+  +VPP IV+TIPRMEVS  GV+ILS
Sbjct: 541 PSGRAALGVHVSAYGDEATGVKSVVPPPIVNTIPRMEVSAPGVEILS 587

BLAST of CsaV3_6G031950 vs. TAIR 10
Match: AT3G47340.1 (glutamine-dependent asparagine synthase 1 )

HSP 1 Score: 1007.3 bits (2603), Expect = 5.5e-294
Identity = 473/576 (82.12%), Postives = 526/576 (91.32%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGCSDDSQAKRVRVLELSRRL+HRGPDWSGLYQ+ D YL+HQRLA++DPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGDNYLAHQRLAVIDPASGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPL+NE+K+IVVTVNGEIYNHEELRK+L+NHK RT SDC+VI+HLYEEYG +FVDMLDG+
Sbjct: 61  QPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVIAHLYEEYGVDFVDMLDGI 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           FSFVLLDTRDNSF+ ARDAIGVTSLYIGWG DGSVWISSE+KGLNDDCEHFETFPPGH Y
Sbjct: 121 FSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFETFPPGHFY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SSK  GFK+WYNP WF+E +PSTPY+PL +RRA ENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKLGGFKQWYNPPWFNESVPSTPYEPLAIRRAFENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VASITAR+L GT+AAK WG QLHSFCVGLEGSPDLKA +EVA++LGTVHHEFHF+VQDGI
Sbjct: 241 VASITARHLAGTKAAKQWGPQLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHFSVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIEDVIYH+ETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGADEIFGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           K+EFH+E+CRKIKALH YDCLRANK+TSA+GLE RVPFLDK FI+ AM++DPE KMIK  
Sbjct: 361 KKEFHQETCRKIKALHKYDCLRANKSTSAFGLEARVPFLDKDFINTAMSLDPESKMIKPE 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
           +GRIEKWVLRRAFDDE++PYLPK++LYRQKEQFSDGVGYSWIDGLK HA Q+V DKM+ N
Sbjct: 421 EGRIEKWVLRRAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAQNVNDKMMSN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           A HIFP NTP +KEAYYYR IFERFFPQNSA LTVPGG ++ACSTAKAVEWDA+WS N+D
Sbjct: 481 AGHIFPHNTPNTKEAYYYRMIFERFFPQNSARLTVPGGATVACSTAKAVEWDASWSNNMD 540

Query: 591 PSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPRM 627
           PSGRAA+GVH S Y D  N    I P K +D +P M
Sbjct: 541 PSGRAAIGVHLSAY-DGKNVALTIPPLKAIDNMPMM 575

BLAST of CsaV3_6G031950 vs. TAIR 10
Match: AT5G10240.1 (asparagine synthetase 3 )

HSP 1 Score: 950.3 bits (2455), Expect = 8.0e-277
Identity = 433/557 (77.74%), Postives = 503/557 (90.31%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGC D+SQAKR R++ELSRRL+HRGPDWSGL+ + DCYL+H+RLAIVDP SGD
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPLYNE+K+I VTVNGEIYNH+ LR+ L++H+ RT SDC+VI+HLYEE+GE FVDMLDGM
Sbjct: 61  QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLYEEHGEEFVDMLDGM 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           F+FVLLDTRD SFIAARDAIG+T LYIGWG DGSVW +SE+K L+DDCE F  FPPGH+Y
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SSK+ G +RWYNP WFSE +PSTPYDPLV+R   E AVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VAS+  R+L  + AA  WG++LH+FC+GL+GSPDLKA REVAD+LGT HHE HFTVQDGI
Sbjct: 241 VASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFTVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIE+VIYH+ETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEG+DEIFGGYLYFHKAPN
Sbjct: 301 DAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           K+EFH E+CRKIKALH YDCLRANK+TSAWG+E RVPFLDK+FI+VAM+IDPEWKMI+ +
Sbjct: 361 KKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWKMIRPD 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
            GRIEKWVLR AFDDE+ PYLPK++LYRQKEQFSDGVGYSWIDGLK HA++HV++ M++N
Sbjct: 421 LGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSETMLMN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           A  +FP NTP +KEAYYYRTIFE+FFP+++A  TVPGGPS+ACSTAKAVEWDAAWS+NLD
Sbjct: 481 ASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAKAVEWDAAWSQNLD 540

Query: 591 PSGRAALGVHASTYGDE 608
           PSGRAALGVH S YG++
Sbjct: 541 PSGRAALGVHVSAYGED 557

BLAST of CsaV3_6G031950 vs. TAIR 10
Match: AT5G65010.1 (asparagine synthetase 2 )

HSP 1 Score: 949.9 bits (2454), Expect = 1.0e-276
Identity = 432/575 (75.13%), Postives = 510/575 (88.70%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGC D+SQAKR R++ELSRRL+HRGPDWSGL+ + DCYL+H+RLAI+DP SGD
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPLYNE+K++ VTVNGEIYNH+ LR+KL++H+ RT SDC+VI+HLYEE+GE F+DMLDGM
Sbjct: 61  QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           F+FVLLDTRD SFIAARDAIG+T LYIGWG DGSVW +SE+K L+DDCE F +FPPGH+Y
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SSK+ G +RWYNP W++E +PSTPYDPLVLR A E AVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VA++  R+L  + AA+ WG+QLH+FC+GL+GSPDLKA REVAD+LGT HHEF FTVQDGI
Sbjct: 241 VAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFTVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIE+VIYHIETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEG+DEI GGYLYFHKAPN
Sbjct: 301 DAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           K+EFH E+CRKIKALH +DCLRANK+TSAWG+E RVPFLDK+F++VAM+IDPEWK+IK +
Sbjct: 361 KKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVAMSIDPEWKLIKPD 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
            GRIEKWVLR AFDDE++PYLPK++LYRQKEQFSDGVGYSWIDGLK HA++HV+D M+ N
Sbjct: 421 LGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSDTMLSN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           A  +FP NTP +KEAYYYRTIFE+FFP+++A  TVPGGPSIACSTAKAVEWDA WSKNLD
Sbjct: 481 ASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSIACSTAKAVEWDATWSKNLD 540

Query: 591 PSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPR 626
           PSGRAALGVH + Y ++           +VD +P+
Sbjct: 541 PSGRAALGVHVAAYEEDKAAAAAKAGSDLVDPLPK 575

BLAST of CsaV3_6G031950 vs. TAIR 10
Match: AT5G65010.2 (asparagine synthetase 2 )

HSP 1 Score: 947.2 bits (2447), Expect = 6.8e-276
Identity = 433/576 (75.17%), Postives = 509/576 (88.37%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGC D+SQAKR R++ELSRRL+HRGPDWSGL+ + DCYL+H+RLAI+DP SGD
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPLYNE+K++ VTVNGEIYNH+ LR+KL++H+ RT SDC+VI+HLYEE+GE F+DMLDGM
Sbjct: 61  QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           F+FVLLDTRD SFIAARDAIG+T LYIGWG DGSVW +SE+K L+DDCE F +FPPGH+Y
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SSK+ G +RWYNP W++E +PSTPYDPLVLR A E AVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VA++  R+L  + AA+ WG+QLH+FC+GL+GSPDLKA REVAD+LGT HHEF FTVQDGI
Sbjct: 241 VAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFTVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIE+VIYHIETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEG+DEI GGYLYFHKAPN
Sbjct: 301 DAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           K+EFH E+CRKIKALH +DCLRANK+TSAWG+E RVPFLDK+F++VAM+IDPEWK+IK +
Sbjct: 361 KKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVAMSIDPEWKLIKPD 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
            GRIEKWVLR AFDDE++PYLPK++LYRQKEQFSDGVGYSWIDGLK HA++HV+D M+ N
Sbjct: 421 LGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSDTMLSN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAC-LTVPGGPSIACSTAKAVEWDAAWSKNL 590
           A  +FP NTP +KEAYYYRTIFE+FFP+ SA   TVPGGPSIACSTAKAVEWDA WSKNL
Sbjct: 481 ASFVFPDNTPLTKEAYYYRTIFEKFFPKQSAARATVPGGPSIACSTAKAVEWDATWSKNL 540

Query: 591 DPSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPR 626
           DPSGRAALGVH + Y ++           +VD +P+
Sbjct: 541 DPSGRAALGVHVAAYEEDKAAAAAKAGSDLVDPLPK 576

BLAST of CsaV3_6G031950 vs. TAIR 10
Match: AT5G10240.2 (asparagine synthetase 3 )

HSP 1 Score: 939.1 bits (2426), Expect = 1.9e-273
Identity = 430/557 (77.20%), Postives = 501/557 (89.95%), Query Frame = 0

Query: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110
           MCGILAVLGC D+SQAKR R++ELSRRL+HRGPDWSGL+ + DCYL+H+RLAIVDP SGD
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60

Query: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170
           QPLYNE+K+I VTVNGEIYNH+ LR+ L++H+ RT SDC+VI+HL  ++GE FVDMLDGM
Sbjct: 61  QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLV-KHGEEFVDMLDGM 120

Query: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230
           F+FVLLDTRD SFIAARDAIG+T LYIGWG DGSVW +SE+K L+DDCE F  FPPGH+Y
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY 180

Query: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290
           SSK+ G +RWYNP WFSE +PSTPYDPLV+R   E AVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350
           VAS+  R+L  + AA  WG++LH+FC+GL+GSPDLKA REVAD+LGT HHE HFTVQDGI
Sbjct: 241 VASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFTVQDGI 300

Query: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410
           DAIE+VIYH+ETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEG+DEIFGGYLYFHKAPN
Sbjct: 301 DAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470
           K+EFH E+CRKIKALH YDCLRANK+TSAWG+E RVPFLDK+FI+VAM+IDPEWKMI+ +
Sbjct: 361 KKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWKMIRPD 420

Query: 471 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILN 530
            GRIEKWVLR AFDDE+ PYLPK++LYRQKEQFSDGVGYSWIDGLK HA++HV++ M++N
Sbjct: 421 LGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSETMLMN 480

Query: 531 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKNLD 590
           A  +FP NTP +KEAYYYRTIFE+FFP+++A  TVPGGPS+ACSTAKAVEWDAAWS+NLD
Sbjct: 481 ASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAKAVEWDAAWSQNLD 540

Query: 591 PSGRAALGVHASTYGDE 608
           PSGRAALGVH S YG++
Sbjct: 541 PSGRAALGVHVSAYGED 556

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8647225.10.0e+00100.00hypothetical protein Csa_018945 [Cucumis sativus][more]
XP_004144212.30.0e+00100.00asparagine synthetase [glutamine-hydrolyzing] isoform X1 [Cucumis sativus][more]
TYK00726.10.0e+0093.49asparagine synthetase [Cucumis melo var. makuwa][more]
XP_004143163.10.0e+0094.04asparagine synthetase [glutamine-hydrolyzing] [Cucumis sativus] >KGN47067.1 hypo... [more]
XP_008465262.10.0e+0094.55PREDICTED: asparagine synthetase [glutamine-hydrolyzing] 1-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
P490921.1e-29481.77Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus OX=34305 GN=A... [more]
O246614.1e-29485.38Asparagine synthetase [glutamine-hydrolyzing] OS=Triphysaria versicolor OX=64093... [more]
P317526.0e-29384.48Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis OX=4686 P... [more]
P490787.8e-29382.12Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Arabidopsis thaliana OX=3702 ... [more]
P490933.0e-29281.15Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Lotus japonicus OX=34305 GN=A... [more]
Match NameE-valueIdentityDescription
A0A5D3BR390.0e+0093.49Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=119... [more]
A0A0A0KF940.0e+0094.04Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis sativus OX=3659 GN=Csa_... [more]
A0A1S3CNG70.0e+0094.55Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo OX=3656 GN=LOC1035... [more]
A0A6J1J8M00.0e+0092.16Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1G8T60.0e+0092.16Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
AT3G47340.15.5e-29482.12glutamine-dependent asparagine synthase 1 [more]
AT5G10240.18.0e-27777.74asparagine synthetase 3 [more]
AT5G65010.11.0e-27675.13asparagine synthetase 2 [more]
AT5G65010.26.8e-27675.17asparagine synthetase 2 [more]
AT5G10240.21.9e-27377.20asparagine synthetase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 245..564
e-value: 3.2E-116
score: 390.3
IPR017932Glutamine amidotransferase type 2 domainPFAMPF13537GATase_7coord: 98..215
e-value: 2.5E-39
score: 134.0
IPR017932Glutamine amidotransferase type 2 domainPROSITEPS51278GATASE_TYPE_2coord: 52..235
score: 34.910107
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 52..242
e-value: 5.1E-57
score: 194.2
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 50..241
IPR001962Asparagine synthasePFAMPF00733Asn_synthasecoord: 260..411
e-value: 9.8E-57
score: 193.2
IPR001962Asparagine synthaseCDDcd01991Asn_Synthase_B_Ccoord: 261..506
e-value: 2.83655E-79
score: 250.414
NoneNo IPR availablePIRSRPIRSR001589-1PIRSR001589-1coord: 51..598
e-value: 1.3E-258
score: 856.8
NoneNo IPR availablePIRSRPIRSR001589-2PIRSR001589-2coord: 51..598
e-value: 1.3E-258
score: 856.8
NoneNo IPR availablePIRSRPIRSR000485-1PIRSR000485-1coord: 51..93
e-value: 0.88
score: 5.8
NoneNo IPR availablePIRSRPIRSR001589-3PIRSR001589-3coord: 51..598
e-value: 1.3E-258
score: 856.8
NoneNo IPR availablePANTHERPTHR11772:SF43ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]coord: 51..635
NoneNo IPR availablePANTHERPTHR11772ASPARAGINE SYNTHETASEcoord: 51..635
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 259..530
IPR033738Asparagine synthase, N-terminal domainCDDcd00712AsnBcoord: 52..242
e-value: 6.9206E-72
score: 229.366

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_6G031950.1CsaV3_6G031950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006529 asparagine biosynthetic process
molecular_function GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity