CsaV3_6G015700 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_6G015700
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	DNA-directed RNA polymerases II and IV subunit 5A-like
Location	chr6: 11664478 .. 11667900 (+)
RNA-Seq Expression	CsaV3_6G015700
Synteny	CsaV3_6G015700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TATGTTATATACTTGCTAGTTTATAGAAAACCATAATAAAAGAAAATGCAAAAGAGAAAAAAAGTTAAAAGAATAAAGGAAAAAATTTAGAAAAAAAAACGCTTATAAAGAAAGAAGAAAACAGAAAAAGGAAAAAGCGGCACAAGGGAGAAGGAAAATAATGCATTTTGGGGTAAAGTAGTAATTTAACTGATTTTTGGTTTGGGCATATAAACGAACCCTCCATGCAATTTTTCCTCCCCTTCTTCACGTTAACTCTTCGCTTTCTCTCTCTTCCATTCATCTTTCTCCTTCTTCCTCCGGCGACCTGTTCGACGGCGTCCGGGGCAATCTCCTCCAACGACAGTGTGGTTCTTCCCTCTCGACAGCACGTACGTGAGCAAGGCGTACGAGCGAGCCGACGGCATTGACGCGAAAACCTCCTTATCGAGCCCAGGGCTCCCACGAACCAGCAACGATCACGCACAGCAGCAGCGTCGAACGTGACTCAGGCGGTTTGCAAACCCTAAAACTGCGTTTTCCCCCATCCTAAAAGGTTTGAAACTTACCCATTTTTGTTCGGCATTATATTTGAAATACCCACTTCTGTGTTGAATGTCTGTGATTTGATGATTCCCTTTTGTTTGGCTTTGATATTCTTATATGCTTTAAGATTAGTCGAGTTGAGGGGAATGTCTATAGATTTTATCTTTCGTTTTCGTTTTTTCCCCCTTTCCCCCTTGTTATTTGGTTTAGGAATTGAGTTGTATACGCTTTTATGTGCATCTTATTGAGAATAATGATTGAATACTGAATTTCTTGAAAATGTGTTTAGGCTGAAAGAGATGGTGTTCCCAGAAGAAGAAATCACCAAACTCTTTAGAGTCCGAAAAACTGTGTTGCAGATGTTGAAAGACAGAGGCTATTTTGTTGGGGATTTCGAGCTTGATATGTCTAAAGTACAATTTAAGAATAAGTTTGGTGAGAACATGAAAAGAGAAGATCTGGTTATCAATAAATCCAAGAGAAATAATAGCTCTGATCAGGTATAGTTTCATACTACACAATGATAATCTCATTTATGTATGACTCTCTTATAACTATTTAGTTTTCAATTTTTAGTTTTTCAAGATTGTGTTTGATGCTCCCAACTCATTGACTATGATTTTCATTTTCTAATGTAAACATTGAATTCGGAGCAAGTTTCAATAGAAAAATGTTTTTAAAAATTACTATTCTTTAGTTTTCAAAACTTGGCTTAAATTTTGAAGACTCACGTAAAAAGAAGAATATAACGAAACATAGAAATCAATAGGTTTAAGTAATATTTACAAGCTTAATTTTCAAGAACCAAAAGCTTAGGGTGTGTTTGGCCCAAGGAGTTGTGAAATATAAGGTAAGAGCTGAGAAGTAGTAGTTGTGAACTTCACTCCTTGTTTGGCTCGAGGAGTTGGTGGGTCCTACTCCTAAATAACTTCAATTTTATATGGTGGACCTCAAAGTTCACAACTCTCTGAGTTTCATTACTCCTTAGTCCTCAATATTTCACTCCGTATCCCAAACACTTGTCTGGCTCACCAACTTCTAAGTTAGTGTTAAATATTTCAACCTCAATGGTTTTCACTACCAAAGTTGCACATTTATTAAGGAACTCCGTCTTGCAATTCTGTAACCCCAAACACATGGTTAATAACTATAACATATTAACTCCAAACTTCCTCACTCAACTTAGCACCCCAACACCCCTCAATTGTTATAAAACAGGGTCTCATTGACTCATGAAATTTATTGTGTATTTAAACATGTTTAAACTTTACCTTAATAAAGGGTTGCAGTAAGTTTCCTCGGAAGAAGTTTCTATTTAAGTCATTCTCAAGTACTGTTACTGTTTGACATGGAAAATAGTTAACTATCGAAAAATATTTACCACATTTATATAAAGTTTGGTTATAGGGATGCACAGGTTATTTGTTAACCCTTTTATTTGTTACCTTCTTTTGATTTATGACTCTTTTACACAGATATATGTTTTCTTTCCTGAAGAGCCGAAGGTTGGTATAAAGATAGTGGAAACTTATGTAGGTCGTATGAAATCAGAGAATGTTTTCAGAGCAATATTTGTTGTTCAACTGAATTTGACCCCCTTTGCCCGAAATAAAATCGCCGAATTATCGTCTTCCAAACTCCATTTGGAAGTTTTCCAGGTAAAAGTAAAATCTCTTATAGTTTTTGGAGCCTTTTAATGTTGAATAGATTATTTTAATGTCATCACATGCTACAAATTTAACGAGAACTAGAGAGGATTTTCTATTTTCCTATCCCAAAACCAGTTCTCTCGTACTTTTGTTGTCTCTCACAACAACAATGATGTGGGGTGGGGGGGGGGGGGGGGGGAGAAGAATGGTGACGTTAGTTTGTAATTATGTGATTTTCAGTTTCCATATAATAATGTCTTGCCTACCTTCTTATTTTGCTGACGTTAACGTTTACCTCTCTGTCATTTTTTTATGCAGGAAACAGAATTGTTGGTTAACATCAAAGAGCATGTTCTCATTCCCGAGCACCAGACACTCACAGATGAAGAGAAGAAAACGTTGTTGGGGAGGTATACGGTGAAAGAAACACAAGTGAGTTTCATTTTATTTGATCTTATTTTCTTGAAATACAAGAATATTGTGAGAATTGTGTTCCATAAAATATGTTGAAATAACTTTGGTAGCTTGGCCCGTGTTGAATAAGATTTTTGTAAATGATGAAGATATTAGGAAATCAGTTCAAGTCATGATGATTGCCTACCTAAAATTTAATTTTCTACACGTTACTCTGACAATCAAATATAGTAACGTTGGATGGTTGTTTTGTTAGAATAGTTAAGATGCGTTCAAGTTGGCTCTAACAGTGTTGGATACATATTGAAAATTGGTATACGTTAGTTTTTAACAAACATTTTGCATTTTGTCTCAGCTACCTCGCATGCAAGTTACCGATCCAATTTCTAGATATTATGGGCTGAAGCGTGGACATGTAGTGAAGATCATCCGACCAAGCGAAACTGCTGGAAGATATGTGACTTATAGATATGTTGTTTGAGATCAATCTGATATGAGTAATTGAAGTCATGATATTTCTGTAGTGGAAGTGGTTTGGAGTTTTAGGCAATTCTCCTTTATGAGCTTCAACTGGGGAATTCGTGCATTTAGAATTTAAATTGAACATCCACCCACGGTTTTTTCTTATTGTTTCATTGACTCACAAGCATGTTTTTTGTAAAGGAAGGGAAAAAACGCTTACTGGTACACCAAATATTGAGGTTTTTGGTGACAAAGAAAGTCGATTAACAAGTTTATCCTTTTGGATCTTTTTGGTTGATCTTTCATTGCTAAAACATTTTCTTATTGTGAGGTTGATGAAAATTTTGAAGGGCCTCCTTTGATTTAAGATAAA

mRNA sequence

ATGGTGTTCCCAGAAGAAGAAATCACCAAACTCTTTAGAGTCCGAAAAACTGTGTTGCAGATGTTGAAAGACAGAGGCTATTTTGTTGGGGATTTCGAGCTTGATATGTCTAAAGTACAATTTAAGAATAAGTTTGGTGAGAACATGAAAAGAGAAGATCTGGTTATCAATAAATCCAAGAGAAATAATAGCTCTGATCAGATATATGTTTTCTTTCCTGAAGAGCCGAAGGTTGGTATAAAGATAGTGGAAACTTATGTAGGTCGTATGAAATCAGAGAATGTTTTCAGAGCAATATTTGTTGTTCAACTGAATTTGACCCCCTTTGCCCGAAATAAAATCGCCGAATTATCGTCTTCCAAACTCCATTTGGAAGTTTTCCAGGAAACAGAATTGTTGGTTAACATCAAAGAGCATGTTCTCATTCCCGAGCACCAGACACTCACAGATGAAGAGAAGAAAACGTTGTTGGGGAGGTATACGGTGAAAGAAACACAACTACCTCGCATGCAAGTTACCGATCCAATTTCTAGATATTATGGGCTGAAGCGTGGACATGTAGTGAAGATCATCCGACCAAGCGAAACTGCTGGAAGATATGTGACTTATAGATATGTTGTTTGA

Coding sequence (CDS)

ATGGTGTTCCCAGAAGAAGAAATCACCAAACTCTTTAGAGTCCGAAAAACTGTGTTGCAGATGTTGAAAGACAGAGGCTATTTTGTTGGGGATTTCGAGCTTGATATGTCTAAAGTACAATTTAAGAATAAGTTTGGTGAGAACATGAAAAGAGAAGATCTGGTTATCAATAAATCCAAGAGAAATAATAGCTCTGATCAGATATATGTTTTCTTTCCTGAAGAGCCGAAGGTTGGTATAAAGATAGTGGAAACTTATGTAGGTCGTATGAAATCAGAGAATGTTTTCAGAGCAATATTTGTTGTTCAACTGAATTTGACCCCCTTTGCCCGAAATAAAATCGCCGAATTATCGTCTTCCAAACTCCATTTGGAAGTTTTCCAGGAAACAGAATTGTTGGTTAACATCAAAGAGCATGTTCTCATTCCCGAGCACCAGACACTCACAGATGAAGAGAAGAAAACGTTGTTGGGGAGGTATACGGTGAAAGAAACACAACTACCTCGCATGCAAGTTACCGATCCAATTTCTAGATATTATGGGCTGAAGCGTGGACATGTAGTGAAGATCATCCGACCAAGCGAAACTGCTGGAAGATATGTGACTTATAGATATGTTGTTTGA

Protein sequence

MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSKRNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSSKLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYYGLKRGHVVKIIRPSETAGRYVTYRYVV*

Homology

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BLAST of CsaV3_6G015700 vs. NCBI nr
Match: XP_004143184.1 (DNA-directed RNA polymerases II and IV subunit 5A [Cucumis sativus] >KGN47027.1 hypothetical protein Csa_020872 [Cucumis sativus])

HSP 1 Score: 403.7 bits (1036), Expect = 9.8e-109
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK
Sbjct: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS
Sbjct: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180
           KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY
Sbjct: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180

Query: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 208
           GLKRGHVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 207

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BLAST of CsaV3_6G015700 vs. NCBI nr
Match: XP_008456369.1 (PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo] >KAA0054557.1 DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 391.3 bits (1004), Expect = 5.0e-105
Identity = 199/207 (96.14%), Postives = 204/207 (98.55%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSK QFKNKFGENMKREDLVINKSK
Sbjct: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKAQFKNKFGENMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS+
Sbjct: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSST 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180
           K+HLEVFQE EL+VNIKEHVLIPEHQ+LTDEEKKTLLGRYTVKETQLPRMQVTDPI+RYY
Sbjct: 121 KVHLEVFQEAELMVNIKEHVLIPEHQSLTDEEKKTLLGRYTVKETQLPRMQVTDPIARYY 180

Query: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 208
           GLKRG VVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 207

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BLAST of CsaV3_6G015700 vs. NCBI nr
Match: TYK20394.1 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 380.9 bits (977), Expect = 6.8e-102
Identity = 199/223 (89.24%), Postives = 204/223 (91.48%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSK QFKNKFGENMKREDLVINKSK
Sbjct: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKAQFKNKFGENMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS+
Sbjct: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSST 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQ-------------- 180
           K+HLEVFQE EL+VNIKEHVLIPEHQ+LTDEEKKTLLGRYTVKETQ              
Sbjct: 121 KVHLEVFQEAELMVNIKEHVLIPEHQSLTDEEKKTLLGRYTVKETQVSFILFDLIFLKYK 180

Query: 181 --LPRMQVTDPISRYYGLKRGHVVKIIRPSETAGRYVTYRYVV 208
             LPRMQVTDPI+RYYGLKRG VVKIIRPSETAGRYVTYRYVV
Sbjct: 181 NILPRMQVTDPIARYYGLKRGQVVKIIRPSETAGRYVTYRYVV 223

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BLAST of CsaV3_6G015700 vs. NCBI nr
Match: XP_008456371.1 (PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 [Cucumis melo])

HSP 1 Score: 354.4 bits (908), Expect = 6.8e-94
Identity = 179/187 (95.72%), Postives = 184/187 (98.40%), Query Frame = 0


Query: 21  MLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSKRNNSSDQIYVFFPEEPKVGI 80
           MLKDRGYFVGDFELDMSK QFKNKFGENMKREDLVINKSKRNNSSDQIYVFFPEEPKVGI
Sbjct: 1   MLKDRGYFVGDFELDMSKAQFKNKFGENMKREDLVINKSKRNNSSDQIYVFFPEEPKVGI 60

Query: 81  KIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSSKLHLEVFQETELLVNIKEHV 140
           KIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS+K+HLEVFQE EL+VNIKEHV
Sbjct: 61  KIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSTKVHLEVFQEAELMVNIKEHV 120

Query: 141 LIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYYGLKRGHVVKIIRPSETAGRY 200
           LIPEHQ+LTDEEKKTLLGRYTVKETQLPRMQVTDPI+RYYGLKRG VVKIIRPSETAGRY
Sbjct: 121 LIPEHQSLTDEEKKTLLGRYTVKETQLPRMQVTDPIARYYGLKRGQVVKIIRPSETAGRY 180

Query: 201 VTYRYVV 208
           VTYRYVV
Sbjct: 181 VTYRYVV 187

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BLAST of CsaV3_6G015700 vs. NCBI nr
Match: XP_018829461.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Juglans regia] >XP_018829463.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Juglans regia] >XP_018829464.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Juglans regia] >XP_041001822.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Juglans microcarpa x Juglans regia] >XP_041001823.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Juglans microcarpa x Juglans regia] >XP_041001824.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Juglans microcarpa x Juglans regia] >KAF5451447.1 hypothetical protein F2P56_026555 [Juglans regia])

HSP 1 Score: 339.0 bits (868), Expect = 3.0e-89
Identity = 169/207 (81.64%), Postives = 190/207 (91.79%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           M   EEEIT+LFR+R+TV+QMLKDRGYFVGDFE+DM+K QFKNK+GE+MKREDLVINKSK
Sbjct: 1   MTLSEEEITRLFRIRRTVMQMLKDRGYFVGDFEIDMTKEQFKNKYGESMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIY+FFPEEPKVG+K ++TY  RMKSENVFRAI VVQ NLTPFAR+ I E+ S+
Sbjct: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYSQRMKSENVFRAILVVQQNLTPFARSSINEI-ST 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180
           K HLEVFQE ELL+NIKEHVL+PEHQ LT+EEKKTLL RYTVKETQLPR+QVTDP++RYY
Sbjct: 121 KFHLEVFQEAELLINIKEHVLVPEHQVLTNEEKKTLLERYTVKETQLPRIQVTDPVARYY 180

Query: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 208
           GLKRG VVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of CsaV3_6G015700 vs. ExPASy Swiss-Prot
Match: O81098 (DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=3702 GN=NRPB5A PE=1 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 2.0e-80
Identity = 150/203 (73.89%), Postives = 177/203 (87.19%), Query Frame = 0


Query: 5   EEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSKRNNS 64
           EEE+ +L+R++KT++QML+DRGYF+ D EL M+K QF  K G+NMKREDLV  K+KRN++
Sbjct: 4   EEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKRNDN 63

Query: 65  SDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSSKLHL 124
           SDQ+Y+FFP+E KVG+K ++ Y  RMKSENVFRAI VVQ NLTPFAR  I+E+ SSK HL
Sbjct: 64  SDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEI-SSKFHL 123

Query: 125 EVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYYGLKR 184
           EVFQE E+LVNIKEHVL+PEHQ LT EEKKTLL RYTVKETQLPR+QVTDPI+RY+GLKR
Sbjct: 124 EVFQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFGLKR 183

Query: 185 GHVVKIIRPSETAGRYVTYRYVV 208
           G VVKIIRPSETAGRYVTYRYVV
Sbjct: 184 GQVVKIIRPSETAGRYVTYRYVV 205

[Close]

BLAST of CsaV3_6G015700 vs. ExPASy Swiss-Prot
Match: Q80UW8 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10090 GN=Polr2e PE=1 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 2.3e-52
Identity = 104/207 (50.24%), Postives = 147/207 (71.01%), Query Frame = 0


Query: 5   EEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGE-----NMKREDLVINKS 64
           EEE  +L+++RKT++Q+  DRGY V   ELD +  +FK +FG+       +R DL +  +
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  KRNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS 124
             ++ +DQ++VFFPEEPKVGIK ++ Y  RM+ EN+ RA+ VVQ  +TP A+  + ++ +
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDM-A 123

Query: 125 SKLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRY 184
            K  LE F + ELL+NI EH L+PEH  +T EE   LL RY ++E+QLPR+Q  DP++RY
Sbjct: 124 PKYVLEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRESQLPRIQAGDPVARY 183

Query: 185 YGLKRGHVVKIIRPSETAGRYVTYRYV 207
           +G+KRG VVKIIRPSETAGRY+TYR V
Sbjct: 184 FGIKRGQVVKIIRPSETAGRYITYRLV 209

[Close]

BLAST of CsaV3_6G015700 vs. ExPASy Swiss-Prot
Match: B0BNE2 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus OX=10116 GN=Polr2e PE=2 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 2.3e-52
Identity = 104/207 (50.24%), Postives = 147/207 (71.01%), Query Frame = 0


Query: 5   EEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGE-----NMKREDLVINKS 64
           EEE  +L+++RKT++Q+  DRGY V   ELD +  +FK +FG+       +R DL +  +
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  KRNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS 124
             ++ +DQ++VFFPEEPKVGIK ++ Y  RM+ EN+ RA+ VVQ  +TP A+  + ++ +
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDM-A 123

Query: 125 SKLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRY 184
            K  LE F + ELL+NI EH L+PEH  +T EE   LL RY ++E+QLPR+Q  DP++RY
Sbjct: 124 PKYVLEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRESQLPRIQAGDPVARY 183

Query: 185 YGLKRGHVVKIIRPSETAGRYVTYRYV 207
           +G+KRG VVKIIRPSETAGRY+TYR V
Sbjct: 184 FGIKRGQVVKIIRPSETAGRYITYRLV 209

[Close]

BLAST of CsaV3_6G015700 vs. ExPASy Swiss-Prot
Match: Q5R587 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=9601 GN=POLR2E PE=2 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 6.7e-52
Identity = 103/207 (49.76%), Postives = 146/207 (70.53%), Query Frame = 0


Query: 5   EEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGE-----NMKREDLVINKS 64
           EEE  +L+++RKT++Q+  DRGY V   ELD +  +F+ +FG+       +R DL +  +
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFRAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  KRNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS 124
             ++ +DQ++VFFPEEPKVGIK ++ Y  RM+ EN+ RA+ VVQ  +TP A+  + ++ +
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDM-A 123

Query: 125 SKLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRY 184
            K  LE F + ELL+NI EH L+PEH  +T EE   LL RY ++E QLPR+Q  DP++RY
Sbjct: 124 PKYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVARY 183

Query: 185 YGLKRGHVVKIIRPSETAGRYVTYRYV 207
           +G+KRG VVKIIRPSETAGRY+TYR V
Sbjct: 184 FGIKRGQVVKIIRPSETAGRYITYRLV 209

[Close]

BLAST of CsaV3_6G015700 vs. ExPASy Swiss-Prot
Match: Q54EH2 (DNA-directed RNA polymerases I, II, and III subunit rpabc1 OS=Dictyostelium discoideum OX=44689 GN=polr2e PE=3 SV=2)

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-51
Identity = 107/203 (52.71%), Postives = 139/203 (68.47%), Query Frame = 0


Query: 6   EEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSKRNNSS 65
           E+I +L ++RKTVLQML DR Y +  +E +  +  F       + RE+L +   K+++ +
Sbjct: 2   EDINRLLQIRKTVLQMLSDREYIITAYEQEYPRESF-------VSRENLTMYCVKKDDPT 61

Query: 66  DQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELS-SSKLHL 125
           D IYVFFP+ PKVG+  ++ YV  MK     RAI VVQ N+TPFA+  +AE S   K+ L
Sbjct: 62  DSIYVFFPDTPKVGVTQIKKYVDIMKDRQANRAIIVVQQNITPFAKQALAEFSQEKKITL 121

Query: 126 EVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYYGLKR 185
           E F E ELLVNI  H L+P+H  LT EEK  LL RY +KE+QLPR+Q  DP++RYYGL R
Sbjct: 122 EQFNEAELLVNITHHQLVPKHILLTKEEKLELLTRYKMKESQLPRIQTNDPVARYYGLSR 181

Query: 186 GHVVKIIRPSETAGRYVTYRYVV 208
           GHVVKI+RPSETAGRY+TYR  V
Sbjct: 182 GHVVKIVRPSETAGRYITYRLCV 197

[Close]

BLAST of CsaV3_6G015700 vs. ExPASy TrEMBL
Match: A0A0A0KF69 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G170410 PE=3 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 4.7e-109
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK
Sbjct: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS
Sbjct: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180
           KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY
Sbjct: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180

Query: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 208
           GLKRGHVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 207

[Close]

BLAST of CsaV3_6G015700 vs. ExPASy TrEMBL
Match: A0A5A7UJ12 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold24G003460 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 2.4e-105
Identity = 199/207 (96.14%), Postives = 204/207 (98.55%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSK QFKNKFGENMKREDLVINKSK
Sbjct: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKAQFKNKFGENMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS+
Sbjct: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSST 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180
           K+HLEVFQE EL+VNIKEHVLIPEHQ+LTDEEKKTLLGRYTVKETQLPRMQVTDPI+RYY
Sbjct: 121 KVHLEVFQEAELMVNIKEHVLIPEHQSLTDEEKKTLLGRYTVKETQLPRMQVTDPIARYY 180

Query: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 208
           GLKRG VVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 207

[Close]

BLAST of CsaV3_6G015700 vs. ExPASy TrEMBL
Match: A0A1S3C2N2 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496327 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 2.4e-105
Identity = 199/207 (96.14%), Postives = 204/207 (98.55%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSK QFKNKFGENMKREDLVINKSK
Sbjct: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKAQFKNKFGENMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS+
Sbjct: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSST 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180
           K+HLEVFQE EL+VNIKEHVLIPEHQ+LTDEEKKTLLGRYTVKETQLPRMQVTDPI+RYY
Sbjct: 121 KVHLEVFQEAELMVNIKEHVLIPEHQSLTDEEKKTLLGRYTVKETQLPRMQVTDPIARYY 180

Query: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 208
           GLKRG VVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 207

[Close]

BLAST of CsaV3_6G015700 vs. ExPASy TrEMBL
Match: A0A5D3DA58 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold519G00160 PE=3 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 3.3e-102
Identity = 199/223 (89.24%), Postives = 204/223 (91.48%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSK QFKNKFGENMKREDLVINKSK
Sbjct: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKAQFKNKFGENMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS+
Sbjct: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSST 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQ-------------- 180
           K+HLEVFQE EL+VNIKEHVLIPEHQ+LTDEEKKTLLGRYTVKETQ              
Sbjct: 121 KVHLEVFQEAELMVNIKEHVLIPEHQSLTDEEKKTLLGRYTVKETQVSFILFDLIFLKYK 180

Query: 181 --LPRMQVTDPISRYYGLKRGHVVKIIRPSETAGRYVTYRYVV 208
             LPRMQVTDPI+RYYGLKRG VVKIIRPSETAGRYVTYRYVV
Sbjct: 181 NILPRMQVTDPIARYYGLKRGQVVKIIRPSETAGRYVTYRYVV 223

[Close]

BLAST of CsaV3_6G015700 vs. ExPASy TrEMBL
Match: A0A1S3C358 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496327 PE=3 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 3.3e-94
Identity = 179/187 (95.72%), Postives = 184/187 (98.40%), Query Frame = 0


Query: 21  MLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSKRNNSSDQIYVFFPEEPKVGI 80
           MLKDRGYFVGDFELDMSK QFKNKFGENMKREDLVINKSKRNNSSDQIYVFFPEEPKVGI
Sbjct: 1   MLKDRGYFVGDFELDMSKAQFKNKFGENMKREDLVINKSKRNNSSDQIYVFFPEEPKVGI 60

Query: 81  KIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSSKLHLEVFQETELLVNIKEHV 140
           KIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS+K+HLEVFQE EL+VNIKEHV
Sbjct: 61  KIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSTKVHLEVFQEAELMVNIKEHV 120

Query: 141 LIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYYGLKRGHVVKIIRPSETAGRY 200
           LIPEHQ+LTDEEKKTLLGRYTVKETQLPRMQVTDPI+RYYGLKRG VVKIIRPSETAGRY
Sbjct: 121 LIPEHQSLTDEEKKTLLGRYTVKETQLPRMQVTDPIARYYGLKRGQVVKIIRPSETAGRY 180

Query: 201 VTYRYVV 208
           VTYRYVV
Sbjct: 181 VTYRYVV 187

[Close]

BLAST of CsaV3_6G015700 vs. TAIR 10
Match: AT3G22320.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 300.1 bits (767), Expect = 1.4e-81
Identity = 150/203 (73.89%), Postives = 177/203 (87.19%), Query Frame = 0


Query: 5   EEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSKRNNS 64
           EEE+ +L+R++KT++QML+DRGYF+ D EL M+K QF  K G+NMKREDLV  K+KRN++
Sbjct: 4   EEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKRNDN 63

Query: 65  SDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSSKLHL 124
           SDQ+Y+FFP+E KVG+K ++ Y  RMKSENVFRAI VVQ NLTPFAR  I+E+ SSK HL
Sbjct: 64  SDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEI-SSKFHL 123

Query: 125 EVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYYGLKR 184
           EVFQE E+LVNIKEHVL+PEHQ LT EEKKTLL RYTVKETQLPR+QVTDPI+RY+GLKR
Sbjct: 124 EVFQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFGLKR 183

Query: 185 GHVVKIIRPSETAGRYVTYRYVV 208
           G VVKIIRPSETAGRYVTYRYVV
Sbjct: 184 GQVVKIIRPSETAGRYVTYRYVV 205

[Close]

BLAST of CsaV3_6G015700 vs. TAIR 10
Match: AT5G57980.1 (RNA polymerase II fifth largest subunit, C )

HSP 1 Score: 181.4 bits (459), Expect = 7.4e-46
Identity = 96/207 (46.38%), Postives = 145/207 (70.05%), Query Frame = 0


Query: 5   EEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENM---KREDLVINKSKR 64
           ++EIT++F+VR+TVLQML+DRGY + + +L++ + +F  +F + M    +E L ++ +K 
Sbjct: 5   DDEITRIFKVRRTVLQMLRDRGYTIEESDLNLKREEFVQRFCKTMNKVNKEALFVSANKG 64

Query: 65  NNSSDQIYVFFPEEPKVGIKIVETYVG-RMKSENVFRAIFVVQLNLTPFARNKIAELSSS 124
            N +D+IYVF+PE PKVG+ +++  V  +M+ + V R I VV + +T  AR  ++EL + 
Sbjct: 65  PNPADKIYVFYPEGPKVGVPVIKKEVAIKMRDDKVHRGIVVVPMAITAPARMAVSEL-NK 124

Query: 125 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 184
            L +EVF+E EL+ NI EH L+ ++  L D+ KK LL  YTV++TQLPR+ VTDP++RYY
Sbjct: 125 MLTIEVFEEAELVTNITEHKLVNKYYVLDDQAKKKLLNTYTVQDTQLPRILVTDPLARYY 184

Query: 185 GLKRGHVVKIIRPSETAGRYVTYRYVV 208
           GLKRG VVKI R   T+  Y TYR+ V
Sbjct: 185 GLKRGQVVKIRRSDATSLDYYTYRFAV 210

[Close]

BLAST of CsaV3_6G015700 vs. TAIR 10
Match: AT2G41340.1 (RNA polymerase II fifth largest subunit, D )

HSP 1 Score: 129.0 bits (323), Expect = 4.3e-30
Identity = 80/201 (39.80%), Postives = 124/201 (61.69%), Query Frame = 0


Query: 7   EITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSKRNNSSD 66
           E  K +  R+T ++ML+DRGY V D ++++S  QF+  +GE+   + L I+   R +SS 
Sbjct: 20  ECHKYYLARRTTMEMLRDRGYDVSDEDINLSLQQFRALYGEHPDVDLLRISAKHRFDSSK 79

Query: 67  QIYVFFPEEPKVGIKIVETYVGRMKS-ENVFRAIFVVQLNLTPFARNKIAELSSSKLHLE 126
           +I V F     V +  +      + S EN+   I V+Q ++T  A  K  EL S K  +E
Sbjct: 80  KISVVFCGTGIVKVNAMRVIAADVLSRENITGLILVLQSHITNQAL-KAVELFSFK--VE 139

Query: 127 VFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYYGLKRG 186
           +F+ T+LLVN+ +HVL P+HQ L D+EK++LL +++++E QLPR+   DPI RYYGL+ G
Sbjct: 140 LFEITDLLVNVSKHVLRPKHQVLNDKEKESLLKKFSIEEKQLPRLSSKDPIVRYYGLETG 199

Query: 187 HVVKIIRPSETAGRYVTYRYV 207
            V+K+    E +  +VTYR V
Sbjct: 200 QVMKVTYKDELSESHVTYRCV 217

[Close]

BLAST of CsaV3_6G015700 vs. TAIR 10
Match: AT3G57080.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 123.6 bits (309), Expect = 1.8e-28
Identity = 74/201 (36.82%), Postives = 115/201 (57.21%), Query Frame = 0


Query: 6   EEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSKRNNSS 65
           EE  + +  R+  LQML+DRGY V D ++++S   F+  +GE    + L I+   R++S+
Sbjct: 23  EESHRYYLARRNGLQMLRDRGYEVSDEDINLSLHDFRTVYGERPDVDRLRISALHRSDST 82

Query: 66  DQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSSKLHLE 125
            ++ + F     V +  + + V  + S+     + +V  N       K  EL S K  +E
Sbjct: 83  KKVKIVFFGTSMVKVNAIRSVVADILSQETITGLILVLQNHVTNQALKAIELFSFK--VE 142

Query: 126 VFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYYGLKRG 185
           +FQ T+LLVNI +H L P+HQ L DEEK TLL +++++E QLPR+   D I RYYGL++G
Sbjct: 143 IFQITDLLVNITKHSLKPQHQVLNDEEKTTLLKKFSIEEKQLPRISKKDAIVRYYGLEKG 202

Query: 186 HVVKIIRPSETAGRYVTYRYV 207
            VVK+    E    +V +R V
Sbjct: 203 QVVKVNYRGELTESHVAFRCV 221

[Close]

BLAST of CsaV3_6G015700 vs. TAIR 10
Match: AT3G54490.1 (RNA polymerase II fifth largest subunit, E )

HSP 1 Score: 105.9 bits (263), Expect = 3.9e-23
Identity = 67/202 (33.17%), Postives = 109/202 (53.96%), Query Frame = 0


Query: 7   EITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSKRNNSSD 66
           E  + +  R T  +ML+DRGY V + EL ++  +F++ FGE  + E L I    R++   
Sbjct: 35  ESKRFYLARTTAFEMLRDRGYEVNEAELSLTLSEFRSVFGEKPELERLRICVPLRSDPKK 94

Query: 67  QIYVFFPEEPKVGIKIVET-YVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSSKLHLE 126
           +I V F     + +K V   ++    +  +   I V+Q  +  FA+     L++    +E
Sbjct: 95  KILVVFMGTEPITVKSVRALHIQISNNVGLHAMILVLQSKMNHFAQK---ALTTFPFTVE 154

Query: 127 VFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYYGLKRG 186
            F   +LLVNI +H+  P+ + L  EEK+ LL ++ +++ QLP +Q  D   RYYGLK+ 
Sbjct: 155 TFPIEDLLVNITKHIQQPKIEILNKEEKEQLLRKHALEDKQLPYLQEKDSFVRYYGLKKK 214

Query: 187 HVVKIIRPSETAGRYVTYRYVV 208
            VVKI    E  G +VTYR ++
Sbjct: 215 QVVKITYSKEPVGDFVTYRCII 233

The following BLAST results are available for this feature:

BLAST of CsaV3_6G015700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143184.1	9.8e-109	100.00	DNA-directed RNA polymerases II and IV subunit 5A [Cucumis sativus] >KGN47027.1 ...	[more]
XP_008456369.1	5.0e-105	96.14	PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cu...	[more]
TYK20394.1	6.8e-102	89.24	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo ...	[more]
XP_008456371.1	6.8e-94	95.72	PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 [Cu...	[more]
XP_018829461.1	3.0e-89	81.64	DNA-directed RNA polymerases II and IV subunit 5A-like [Juglans regia] >XP_01882...	[more]

BLAST of CsaV3_6G015700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81098	2.0e-80	73.89	DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=370...	[more]
Q80UW8	2.3e-52	50.24	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10...	[more]
B0BNE2	2.3e-52	50.24	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus ...	[more]
Q5R587	6.7e-52	49.76	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=96...	[more]
Q54EH2	2.0e-51	52.71	DNA-directed RNA polymerases I, II, and III subunit rpabc1 OS=Dictyostelium disc...	[more]

BLAST of CsaV3_6G015700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KF69	4.7e-109	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G170410 PE=3 SV=1	[more]
A0A5A7UJ12	2.4e-105	96.14	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis mel...	[more]
A0A1S3C2N2	2.4e-105	96.14	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis mel...	[more]
A0A5D3DA58	3.3e-102	89.24	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis mel...	[more]
A0A1S3C358	3.3e-94	95.72	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 OS=Cucumis mel...	[more]

BLAST of CsaV3_6G015700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G22320.1	1.4e-81	73.89	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT5G57980.1	7.4e-46	46.38	RNA polymerase II fifth largest subunit, C	[more]
AT2G41340.1	4.3e-30	39.80	RNA polymerase II fifth largest subunit, D	[more]
AT3G57080.1	1.8e-28	36.82	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT3G54490.1	3.9e-23	33.17	RNA polymerase II fifth largest subunit, E	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005571	RNA polymerase, Rpb5, N-terminal	PFAM	PF03871	RNA_pol_Rpb5_N	coord: 5..90 e-value: 2.1E-29 score: 102.0
IPR035913	RPB5-like RNA polymerase subunit superfamily	GENE3D	3.90.940.20		coord: 132..207 e-value: 5.8E-32 score: 111.2
IPR035913	RPB5-like RNA polymerase subunit superfamily	SUPERFAMILY	55287	RPB5-like RNA polymerase subunit	coord: 132..207
IPR014381	DNA-directed RNA polymerase RPB5 subunit, eukaryote/virus	PIRSF	PIRSF000747	RPB5	coord: 2..207 e-value: 2.6E-82 score: 273.3
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	GENE3D	3.40.1340.10		coord: 4..131 e-value: 5.1E-44 score: 151.7
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	SUPERFAMILY	53036	Eukaryotic RPB5 N-terminal domain	coord: 5..135
IPR000783	RNA polymerase, subunit H/Rpb5 C-terminal	PFAM	PF01191	RNA_pol_Rpb5_C	coord: 134..206 e-value: 2.0E-32 score: 110.9
IPR039531	DNA-directed RNA polymerase subunit Rpb5-like	PANTHER	PTHR10535	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1	coord: 1..207
None	No IPR available	PANTHER	PTHR10535:SF17	DNA-DIRECTED RNA POLYMERASES II AND IV SUBUNIT 5A-LIKE	coord: 1..207
IPR020609	Archaeal RpoH /eukaryotic RPB5 RNA polymerase subunit	HAMAP	MF_00025	RNApol_RpoH_RPB5	coord: 134..207 score: 21.908932

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_6G015700.1	CsaV3_6G015700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity