CsaV3_6G006500 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_6G006500
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionRING-type E3 ubiquitin transferase
Locationchr6: 5405955 .. 5407839 (-)
RNA-Seq ExpressionCsaV3_6G006500
SyntenyCsaV3_6G006500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTAATGTTGCCTTCAATAATCTACCCAACTACCTCTCTTTTCAATTCTTCCACCGTCTTCAATGGAGGATATTCACCAAAATCCTTTTCCTCCACCGCACGCACCGACCCCCACCACTGTGGACTTTCCGCCACACTTCCGATGCCCGATTTCCATGGAGCTCATGCAAGATCCTGTCACGATTTCCACCGGAGTTTCCTTTGAAAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCTGCAACCATGCAAACCATTGTCAACTTTGATATCACACCAAATTATACGCTCAAAAGGCTCATACTTTCTTGGAAAATGAAAGATCAGTCTTGCTCCTCCACGTCGTCGTTGTTGCCATCGTCCTCGATGGAGTATCATGATGTTGTGGCTGATCTCTTGAAGAACATCGAGTCGTCGCCGTTCAAGGCGAGTTCTTTGAAGAAACTGCGATTGCTCATGGCGGTGGATGATGTTGCTGCTGTTGGAGCGAAACCCGTCTTTGTTCGAGTAAATGGGTTTGAAGTTCTTATCGGAATAGTGACGCAAGTCGCCATTTGTGAGAGCTCCGATTTTGCTAACTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTGTTTCAAAAACTGGTCACCTCTAAAGTTTAACTTTTTTTTTTTCTTTAGTTAACATTTAAGTGATCGTTGAAATACAGACTATAATCTATTCGATTTAAAATACATGTTTTAACTAATTGAACCTATAAATTTATTTGGTCAAACATTTACACTTTATTTATATTTATTAACAGAAGATTTACGTTTATAGATACCGATATTAATAATTAGAAATGAGGAATATCACTGAAGAGATGAGACTAGCTCTTCAAAAGCTTAAATATTTGAAGGGATAGATAGTGATATATTCAATCTTTTATTCATATTCTTAACACAGGTATTATCTCAATTCCCATTTTCCAAAACCGAGAAGCCATTCGAGTTGCTATCAGAACCAGAAGCCATCAAATCAATGGCGATTGTCTTACAAAGAGGAAGCATAGAAGGAAGATTTTATGCCATGGAAATGCTCCAGCAAATATCCAAAAAGGGCTACGATTGGAACTCTTTGACGAAGCATCAAATCATTGATTTATTCAAATCAATATTGGAGCTTGCATCAGATGAGATAATCAAGAAAGCAAGTGCAATCAAGACAACGGATTCCTCGATAGTTACACACTGGTACAGTATCATCGTCCACATGCATATTTTCATTTTATATACAGCATCACTTTTAGTTGCTAATTAACCGTTTCTTTTCTTAGTCGATATGGGACATTGTTCTCGTCCGCCCTAGAGCTTTTGATAGAAATCATGGAATCGTCGAAAAAAAGTCGTCTTTTATCGATTGAGGCTGGCGCTGTTTGCGTCATGATTGATCTTCTTCCGGACTCAAACCGATCGAAGTGCGAGAAAATACTACACATACTCAAACTTCTAAGTGAGTGTGCCGAGGGAAGGTCAGCCATGGGAGAACATATTATGGGAATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATCTTGTGGTTGATCTGTAATTATCATCCAAGTGAGAGGGTTTTAGAAGAAATGATGGGGTGTGGGACAGTGAAGAAGCTTTTAGGGATCTTGAATAATGGTGATGGAAGATCTTCAACTAAGGAGAAGGCCAAGAAGGTTATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAGTATAGGGATTATTTGAAGTTGATAAATCAATAGAATATACATTGAAATGAATGACAATTGGAATTTGGAGAATATATATTAGAATACAAATTTAG

mRNA sequence

ATGGAGGATATTCACCAAAATCCTTTTCCTCCACCGCACGCACCGACCCCCACCACTGTGGACTTTCCGCCACACTTCCGATGCCCGATTTCCATGGAGCTCATGCAAGATCCTGTCACGATTTCCACCGGAGTTTCCTTTGAAAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCTGCAACCATGCAAACCATTGTCAACTTTGATATCACACCAAATTATACGCTCAAAAGGCTCATACTTTCTTGGAAAATGAAAGATCAGTCTTGCTCCTCCACGTCGTCGTTGTTGCCATCGTCCTCGATGGAGTATCATGATGTTGTGGCTGATCTCTTGAAGAACATCGAGTCGTCGCCGTTCAAGGCGAGTTCTTTGAAGAAACTGCGATTGCTCATGGCGGTGGATGATGTTGCTGCTGTTGGAGCGAAACCCGTCTTTGTTCGAGTAAATGGGTTTGAAGTTCTTATCGGAATAGTGACGCAAGTCGCCATTTGTGAGAGCTCCGATTTTGCTAACTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTATCTCAATTCCCATTTTCCAAAACCGAGAAGCCATTCGAGTTGCTATCAGAACCAGAAGCCATCAAATCAATGGCGATTGTCTTACAAAGAGGAAGCATAGAAGGAAGATTTTATGCCATGGAAATGCTCCAGCAAATATCCAAAAAGGGCTACGATTGGAACTCTTTGACGAAGCATCAAATCATTGATTTATTCAAATCAATATTGGAGCTTGCATCAGATGAGATAATCAAGAAAGCAAGTGCAATCAAGACAACGGATTCCTCGATAGTTACACACTGTCGATATGGGACATTGTTCTCGTCCGCCCTAGAGCTTTTGATAGAAATCATGGAATCGTCGAAAAAAAGTCGTCTTTTATCGATTGAGGCTGGCGCTGTTTGCGTCATGATTGATCTTCTTCCGGACTCAAACCGATCGAAGTGCGAGAAAATACTACACATACTCAAACTTCTAAGTGAGTGTGCCGAGGGAAGGTCAGCCATGGGAGAACATATTATGGGAATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATCTTGTGGTTGATCTGTAATTATCATCCAAGTGAGAGGGTTTTAGAAGAAATGATGGGGTGTGGGACAGTGAAGAAGCTTTTAGGGATCTTGAATAATGGTGATGGAAGATCTTCAACTAAGGAGAAGGCCAAGAAGGTTATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAGTATAGGGATTATTTGAAGTTGATAAATCAATAG

Coding sequence (CDS)

ATGGAGGATATTCACCAAAATCCTTTTCCTCCACCGCACGCACCGACCCCCACCACTGTGGACTTTCCGCCACACTTCCGATGCCCGATTTCCATGGAGCTCATGCAAGATCCTGTCACGATTTCCACCGGAGTTTCCTTTGAAAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCTGCAACCATGCAAACCATTGTCAACTTTGATATCACACCAAATTATACGCTCAAAAGGCTCATACTTTCTTGGAAAATGAAAGATCAGTCTTGCTCCTCCACGTCGTCGTTGTTGCCATCGTCCTCGATGGAGTATCATGATGTTGTGGCTGATCTCTTGAAGAACATCGAGTCGTCGCCGTTCAAGGCGAGTTCTTTGAAGAAACTGCGATTGCTCATGGCGGTGGATGATGTTGCTGCTGTTGGAGCGAAACCCGTCTTTGTTCGAGTAAATGGGTTTGAAGTTCTTATCGGAATAGTGACGCAAGTCGCCATTTGTGAGAGCTCCGATTTTGCTAACTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTATCTCAATTCCCATTTTCCAAAACCGAGAAGCCATTCGAGTTGCTATCAGAACCAGAAGCCATCAAATCAATGGCGATTGTCTTACAAAGAGGAAGCATAGAAGGAAGATTTTATGCCATGGAAATGCTCCAGCAAATATCCAAAAAGGGCTACGATTGGAACTCTTTGACGAAGCATCAAATCATTGATTTATTCAAATCAATATTGGAGCTTGCATCAGATGAGATAATCAAGAAAGCAAGTGCAATCAAGACAACGGATTCCTCGATAGTTACACACTGTCGATATGGGACATTGTTCTCGTCCGCCCTAGAGCTTTTGATAGAAATCATGGAATCGTCGAAAAAAAGTCGTCTTTTATCGATTGAGGCTGGCGCTGTTTGCGTCATGATTGATCTTCTTCCGGACTCAAACCGATCGAAGTGCGAGAAAATACTACACATACTCAAACTTCTAAGTGAGTGTGCCGAGGGAAGGTCAGCCATGGGAGAACATATTATGGGAATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATCTTGTGGTTGATCTGTAATTATCATCCAAGTGAGAGGGTTTTAGAAGAAATGATGGGGTGTGGGACAGTGAAGAAGCTTTTAGGGATCTTGAATAATGGTGATGGAAGATCTTCAACTAAGGAGAAGGCCAAGAAGGTTATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAGTATAGGGATTATTTGAAGTTGATAAATCAATAG

Protein sequence

MEDIHQNPFPPPHAPTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEKAKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ*
Homology
BLAST of CsaV3_6G006500 vs. NCBI nr
Match: XP_004145072.2 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN46274.1 hypothetical protein Csa_004999 [Cucumis sativus])

HSP 1 Score: 894.0 bits (2309), Expect = 5.2e-256
Identity = 451/451 (100.00%), Postives = 451/451 (100.00%), Query Frame = 0

Query: 1   MEDIHQNPFPPPHAPTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN 60
           MEDIHQNPFPPPHAPTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN
Sbjct: 1   MEDIHQNPFPPPHAPTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN 60

Query: 61  KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKN 120
           KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKN
Sbjct: 61  KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKN 120

Query: 121 IESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFE 180
           IESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFE
Sbjct: 121 IESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFE 180

Query: 181 ACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGY 240
           ACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGY
Sbjct: 181 ACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGY 240

Query: 241 DWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEIM 300
           DWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEIM
Sbjct: 241 DWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEIM 300

Query: 301 ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT 360
           ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT
Sbjct: 301 ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT 360

Query: 361 VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEK 420
           VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEK
Sbjct: 361 VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEK 420

Query: 421 AKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           AKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ
Sbjct: 421 AKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

BLAST of CsaV3_6G006500 vs. NCBI nr
Match: XP_008460073.1 (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo])

HSP 1 Score: 828.6 bits (2139), Expect = 2.7e-236
Identity = 426/454 (93.83%), Postives = 436/454 (96.04%), Query Frame = 0

Query: 1   MEDIHQNPFPPPHAPTP---TTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQ+PFPP H P+P    +VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADL 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK +SCSST SLLPSSSMEYHDVVADL
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK-ESCSST-SLLPSSSMEYHDVVADL 120

Query: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFA 180
           LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFE+L+ IVTQVAICE SDFA
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFA 180

Query: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAI+LQRGSIEGRFYAMEMLQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLI 300
           KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKT DSS +T CRYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360
           EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKIL+ILKLLSECAEGRSAMGEH MG
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMG 360

Query: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSST 420
           IATVTKKMSISNTATKIGMKILW IC+YHPSERVLEEMMGCGTVKKLLGIL NGDG SST
Sbjct: 361 IATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGIL-NGDGSSST 420

Query: 421 KEKAKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           KEKA KVMKLHGSFWRRFPCFPYEYRDYLKLINQ
Sbjct: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

BLAST of CsaV3_6G006500 vs. NCBI nr
Match: XP_038875803.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 811.6 bits (2095), Expect = 3.4e-231
Identity = 415/453 (91.61%), Postives = 430/453 (94.92%), Query Frame = 0

Query: 1   MEDIHQNPFPPPHAPTP--TTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDIHQN  PPP  P P  T VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIHQNLLPPPQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLL 120
           YNKKTCPATMQTIVNFDITPNYTLKRLILSWK K+ S SS+SS  PSSSM+Y DVVADLL
Sbjct: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSSSSSSS--PSSSMKYDDVVADLL 120

Query: 121 KNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFAN 180
           KNIES+PFKASSLKKLRLLMA+DDVAAVGAKPVFVR NGF+VL+GI+TQVAICES+DFAN
Sbjct: 121 KNIESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSKTEKPFELLS PEAIKSMAIVLQRGS+EGRFYAMEMLQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKK 240

Query: 241 GYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIE 300
            YDWNSLTKH IIDLFKSILELASDEIIKKAS IKTT+ SI+T CRYGTLFSSALELLIE
Sbjct: 241 EYDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI
Sbjct: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTK 420
           ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGIL NGDGRSSTK
Sbjct: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGIL-NGDGRSSTK 420

Query: 421 EKAKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           EKA K+MKLHGSFWRRFPCFPYE+RDYLKLINQ
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 450

BLAST of CsaV3_6G006500 vs. NCBI nr
Match: XP_023549278.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 753.4 bits (1944), Expect = 1.1e-213
Identity = 392/454 (86.34%), Postives = 412/454 (90.75%), Query Frame = 0

Query: 1   MEDIHQNPFPP--PHAPTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDI QN  PP  P  P P   D PP+FRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPS-SSMEYHDVVADL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ++SCSS SS  P   SMEY DVVA L
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKM-NESCSSASSSSPGPPSMEYDDVVA-L 120

Query: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFA 180
           LKNIE SPFKASSLKKLRLLMA+DDVAAVGAKP+FV+ NGFEVL+G+VTQVAICESS+FA
Sbjct: 121 LKNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFA 180

Query: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVLSQFPFSKTEK FELLS PEAIKSMAIVLQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLI 300
           KGYD+NSLTKH  IDLFKSILELASDEIIKKAS +K TDSSI T CRYGTLFSSALELLI
Sbjct: 241 KGYDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360
           EIMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMG
Sbjct: 301 EIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360

Query: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGR-SS 420
           IATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMMGCGTVKKL+GIL NGDGR SS
Sbjct: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGIL-NGDGRSSS 420

Query: 421 TKEKAKKVMKLHGSFWRRFPCFPYEYRDYLKLIN 451
           TKEKA K+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 TKEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

BLAST of CsaV3_6G006500 vs. NCBI nr
Match: XP_022958929.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata])

HSP 1 Score: 748.8 bits (1932), Expect = 2.7e-212
Identity = 387/454 (85.24%), Postives = 411/454 (90.53%), Query Frame = 0

Query: 1   MEDIHQNPFPPPH--APTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDI QN  PPP    P P   D PP+FRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLP-SSSMEYHDVVADL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ++SCSS SS  P   SMEY DVVA L
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKM-NESCSSASSSSPRPPSMEYDDVVA-L 120

Query: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFA 180
           LKNIE SPFKASSLKKLRLLMA+DDV AVGAKP+FV+ NGFEVL+G+VTQ+AICESS+FA
Sbjct: 121 LKNIELSPFKASSLKKLRLLMAIDDVVAVGAKPIFVQANGFEVLVGLVTQIAICESSNFA 180

Query: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVLSQFPFSK EK FELL+ PEAIKSMAIVLQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLI 300
           KGYD+NSL+KH  IDLFKSILELASDEIIKKAS +K TDSSI THCRYGTLFSSALELLI
Sbjct: 241 KGYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360
           EIMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMG
Sbjct: 301 EIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360

Query: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGR-SS 420
           IATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEM+GCGTVKKL+GIL NGDGR SS
Sbjct: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMIGCGTVKKLVGIL-NGDGRSSS 420

Query: 421 TKEKAKKVMKLHGSFWRRFPCFPYEYRDYLKLIN 451
           TKEKA K+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 TKEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

BLAST of CsaV3_6G006500 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 7.0e-46
Identity = 133/427 (31.15%), Postives = 212/427 (49.65%), Query Frame = 0

Query: 20  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 79
           ++ PP F CPIS+E+M+DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 80  YTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKNIESSPFKASSLKKLRLLMA 139
           +TL+RLI SW   + S        P   +   ++   +  +  S   +   LK+LR +++
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVS 129

Query: 140 VDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFEACEEALGVLSQFPFSKT-- 199
            +       K         E L  IV       S+D  N    +EAL +L     S+T  
Sbjct: 130 EN----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSETVL 189

Query: 200 EKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHQIIDLFKSI 259
           +       +   +KS+  ++QRG  E R YA  +L+ I +      S+T     ++F  +
Sbjct: 190 KNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVFTEV 249

Query: 260 LELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEIMESSKKSRLLSIEAGAVC 319
           +++  D I +KA+  K     +V  C +G                  ++R  ++EAG + 
Sbjct: 250 VQILDDRISQKAT--KAAMHILVNICPWG------------------RNRHKAVEAGVIS 309

Query: 320 VMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK-MSISNTAT 379
           V+I+LL D    S R   E  + +L LL +CAEGR+    H   IA V KK + +S TA+
Sbjct: 310 VIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTAS 369

Query: 380 KIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEKAKKVMKLHGSFW 439
              +++L  +  +  +  +L EM+  G V KL  +L    G   TKEKAK+++KLH   W
Sbjct: 370 DRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCG-GKTKEKAKELLKLHARVW 402

BLAST of CsaV3_6G006500 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 4.0e-41
Identity = 125/450 (27.78%), Postives = 225/450 (50.00%), Query Frame = 0

Query: 20  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 79
           ++ P  F CPIS+++M+DPV +STG++++R +IEKW F+  K +CP T Q I   D+TPN
Sbjct: 5   IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPN 64

Query: 80  YTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKNIESSPFKASSLKKLRLLMA 139
           +TL+RLI SW   + S        P   +   ++   + ++  S   +   LK+LR +++
Sbjct: 65  HTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVS 124

Query: 140 VD-------DVAAVGAKPVFVRVNGFEVLIGIVTQVA------ICESSDFAN-FEA---- 199
            +       + A V      +  N  +      + ++      +C+S+   N F++    
Sbjct: 125 ENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSL 184

Query: 200 CEEALGVLSQFPFSKTEKPFELLSE--PEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKG 259
            +EAL VL     S+T     L ++     +K++  ++QRG  E R YA  +L+++ +  
Sbjct: 185 MDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVA 244

Query: 260 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEI 319
                +   +  +LF  ++++  D+I  KA+  ++    +V  C +G             
Sbjct: 245 DPMQIILLER--ELFGEVIQILHDQISHKAT--RSAMQILVITCPWG------------- 304

Query: 320 MESSKKSRLLSIEAGAVCVMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGEHI 379
                ++R  ++E G + ++I+LL D    S R   E  + +L +L +CAEGR+    H 
Sbjct: 305 -----RNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHG 364

Query: 380 MGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGR 439
             IA V+KK + +S   ++  +++L  +  +  +  +L+EM+  G V KL  +L    G 
Sbjct: 365 AAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCG- 424

Query: 440 SSTKEKAKKVMKLHGSFWRRFPCFPYEYRD 445
           + TKEKAK+++KLH   WR  PC P    D
Sbjct: 425 NKTKEKAKELLKLHARVWRESPCVPRNLYD 431

BLAST of CsaV3_6G006500 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 5.2e-33
Identity = 116/441 (26.30%), Postives = 214/441 (48.53%), Query Frame = 0

Query: 20  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTI-VNFDITP 79
           ++ P +F CPIS+E+M+DPVT  +G++++R NI KW       +CP T Q + ++ D+TP
Sbjct: 8   IEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTP 67

Query: 80  NYTLKRLILSWKMKDQS---CSSTSSLLPSSSMEYHDVVADLLKNIESSPFKASSLKKLR 139
           N+ L+RLI  W +++++      ++  +P   +   + + +L K  + +  +  +L+KL 
Sbjct: 68  NHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLE 127

Query: 140 LLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFEACEEALGVLSQFPFS 199
           +L A+D       + +       + LI  V +    +       +  +E+L +L      
Sbjct: 128 VL-AMDG----NNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIP 187

Query: 200 KTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHQIIDLFK 259
             +    L+     ++S+  VL +     + Y + +L+ +++  Y  + + +    ++FK
Sbjct: 188 SNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFK 247

Query: 260 SILELASDEI----------------IKKASAIKTTDSSIVTHCRYGTLFSSALELLIEI 319
            I+    D +                  + S  KT  S +          ++AL +L+E 
Sbjct: 248 GIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLE- 307

Query: 320 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSK--CEKILHILKLLSECAEGRSAMGEHIMG 379
             S  ++R L ++ GAV  +I+L       K   E +L +L  L  CA GR+ +  H  G
Sbjct: 308 TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGG 367

Query: 380 IATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSS 438
           IA VTK+ + +S  A    + IL  +  + P   V+EEM+  GTV+KL  +L    G  +
Sbjct: 368 IAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG-LN 427

BLAST of CsaV3_6G006500 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 8.9e-33
Identity = 124/434 (28.57%), Postives = 203/434 (46.77%), Query Frame = 0

Query: 15  PTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNF 74
           P    +  P HFRCPIS+ELMQDPVT+ TG +++RA+IE W    N  TCP T   + +F
Sbjct: 7   PLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDF 66

Query: 75  DITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLK------NIESSPFKA 134
            + PN+TL+RLI  W + ++S        P    +   V A L +         S   +A
Sbjct: 67  TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRA 126

Query: 135 SSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFEACEEALGV 194
           ++L++LR      D   V    +    N  E+LI I+        S+  + E   E+L +
Sbjct: 127 AALRRLRGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELVSESLAL 186

Query: 195 LSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQIS--KKGYDWNSLT 254
           L   P ++  +   + S+P  ++ +  +L   SIE R  A  +++ +S   K  D     
Sbjct: 187 LVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSI 246

Query: 255 KHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEIMESSKKS 314
            +    +F+ +L+L  + I  +  A+K    ++   C                  S K +
Sbjct: 247 SNS-ESVFEGVLDLLRNPISSR-RALKIGIKTLFALC------------------SVKST 306

Query: 315 RLLSIEAGAVCVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK- 374
           R ++I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K  
Sbjct: 307 RHIAITAGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTI 366

Query: 375 MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEKAKKV 434
           + +S+ AT+     L  +C     ER  EE  G G V +LL ++   +     K+KA+K+
Sbjct: 367 LRVSDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLL-LMVQSECTERAKKKAQKL 406

Query: 435 MKLHGSFWRRFPCF 439
           +KL    W  +  F
Sbjct: 427 LKLLRDSWPDYNSF 406

BLAST of CsaV3_6G006500 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 5.6e-27
Identity = 121/430 (28.14%), Postives = 201/430 (46.74%), Query Frame = 0

Query: 15  PTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNF 74
           P    +  P HFRCPIS++LM DPVTISTG +++R +I+ W    N  TCP T   + +F
Sbjct: 7   PLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVALSDF 66

Query: 75  DITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDV-VADLLKNIE-------SSPF 134
            + PN+TL+RLI  W + ++  S+    +P+       + V  LL           S   
Sbjct: 67  TLIPNHTLRRLIQEWCVANR--SNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRS 126

Query: 135 KASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFEACEEAL 194
           +A+++++LR L    +   V    +    N  E+L+ I+   A  E++  ++ E   E+L
Sbjct: 127 RAAAIRRLRGLARDSEKNRV----LIAGHNAREILVRIL--FADIETTSLSS-ELVSESL 186

Query: 195 GVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQI--SKKGYDWNS 254
            +L     ++TE    + S+P  +  M  +L   SIE R  A  +++ +    K  D   
Sbjct: 187 ALLVLLHMTETECE-AVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKL 246

Query: 255 LTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEIMESSK 314
           +       +F+ +L+L  + I  +  A+K    +I   C                    K
Sbjct: 247 IISGS-DSIFEGVLDLLKNPISSR-RALKIGIKAIFALCLV------------------K 306

Query: 315 KSRLLSIEAGAVCVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTK 374
           ++R L+I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K
Sbjct: 307 QTRHLAISAGAPGILIDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVK 366

Query: 375 K-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEKAK 433
             + +S+ AT+     L  +C     ER  +E    G V +LL +L   D     K KA+
Sbjct: 367 TILRVSDRATEYAAGALLALCT--AEERCRDEAAAAGLVTQLL-LLVQSDCTERAKRKAQ 402

BLAST of CsaV3_6G006500 vs. ExPASy TrEMBL
Match: A0A0A0KBP6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE=4 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 2.5e-256
Identity = 451/451 (100.00%), Postives = 451/451 (100.00%), Query Frame = 0

Query: 1   MEDIHQNPFPPPHAPTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN 60
           MEDIHQNPFPPPHAPTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN
Sbjct: 1   MEDIHQNPFPPPHAPTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN 60

Query: 61  KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKN 120
           KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKN
Sbjct: 61  KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKN 120

Query: 121 IESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFE 180
           IESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFE
Sbjct: 121 IESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFE 180

Query: 181 ACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGY 240
           ACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGY
Sbjct: 181 ACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGY 240

Query: 241 DWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEIM 300
           DWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEIM
Sbjct: 241 DWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEIM 300

Query: 301 ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT 360
           ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT
Sbjct: 301 ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT 360

Query: 361 VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEK 420
           VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEK
Sbjct: 361 VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEK 420

Query: 421 AKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           AKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ
Sbjct: 421 AKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

BLAST of CsaV3_6G006500 vs. ExPASy TrEMBL
Match: A0A1S3CC54 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.3e-236
Identity = 426/454 (93.83%), Postives = 436/454 (96.04%), Query Frame = 0

Query: 1   MEDIHQNPFPPPHAPTP---TTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQ+PFPP H P+P    +VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADL 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK +SCSST SLLPSSSMEYHDVVADL
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK-ESCSST-SLLPSSSMEYHDVVADL 120

Query: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFA 180
           LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFE+L+ IVTQVAICE SDFA
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFA 180

Query: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAI+LQRGSIEGRFYAMEMLQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLI 300
           KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKT DSS +T CRYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360
           EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKIL+ILKLLSECAEGRSAMGEH MG
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMG 360

Query: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSST 420
           IATVTKKMSISNTATKIGMKILW IC+YHPSERVLEEMMGCGTVKKLLGIL NGDG SST
Sbjct: 361 IATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGIL-NGDGSSST 420

Query: 421 KEKAKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           KEKA KVMKLHGSFWRRFPCFPYEYRDYLKLINQ
Sbjct: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

BLAST of CsaV3_6G006500 vs. ExPASy TrEMBL
Match: A0A6J1H6I8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075 PE=4 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 1.3e-212
Identity = 387/454 (85.24%), Postives = 411/454 (90.53%), Query Frame = 0

Query: 1   MEDIHQNPFPPPH--APTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDI QN  PPP    P P   D PP+FRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLP-SSSMEYHDVVADL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ++SCSS SS  P   SMEY DVVA L
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKM-NESCSSASSSSPRPPSMEYDDVVA-L 120

Query: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFA 180
           LKNIE SPFKASSLKKLRLLMA+DDV AVGAKP+FV+ NGFEVL+G+VTQ+AICESS+FA
Sbjct: 121 LKNIELSPFKASSLKKLRLLMAIDDVVAVGAKPIFVQANGFEVLVGLVTQIAICESSNFA 180

Query: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVLSQFPFSK EK FELL+ PEAIKSMAIVLQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLI 300
           KGYD+NSL+KH  IDLFKSILELASDEIIKKAS +K TDSSI THCRYGTLFSSALELLI
Sbjct: 241 KGYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360
           EIMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMG
Sbjct: 301 EIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360

Query: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGR-SS 420
           IATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEM+GCGTVKKL+GIL NGDGR SS
Sbjct: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMIGCGTVKKLVGIL-NGDGRSSS 420

Query: 421 TKEKAKKVMKLHGSFWRRFPCFPYEYRDYLKLIN 451
           TKEKA K+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 TKEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

BLAST of CsaV3_6G006500 vs. ExPASy TrEMBL
Match: A0A5D3DLY2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00740 PE=4 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 2.5e-211
Identity = 391/454 (86.12%), Postives = 402/454 (88.55%), Query Frame = 0

Query: 1   MEDIHQNPFPPPHAPTP---TTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQ+PFPP H P+P    +VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADL 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK +SCSST SLLPSSSMEYHDVVADL
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK-ESCSST-SLLPSSSMEYHDVVADL 120

Query: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFA 180
           LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFE+L+ IVTQVAICE SDF 
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFT 180

Query: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAI+LQRGSIEGRFYAMEMLQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLI 300
           KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSS +T CRYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSTITQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360
           EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKIL+ILKLLSECAEG          
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEG---------- 360

Query: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSST 420
                                     +YHPSERVLEEMMGCGTVKKLLGIL NGDG SST
Sbjct: 361 --------------------------SYHPSERVLEEMMGCGTVKKLLGIL-NGDGSSST 415

Query: 421 KEKAKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           KEKA KVMKLHGSFWR+FPCFPYEYRDYLKLINQ
Sbjct: 421 KEKAMKVMKLHGSFWRKFPCFPYEYRDYLKLINQ 415

BLAST of CsaV3_6G006500 vs. ExPASy TrEMBL
Match: A0A6J1KWQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 PE=4 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 3.0e-209
Identity = 385/452 (85.18%), Postives = 408/452 (90.27%), Query Frame = 0

Query: 1   MEDIHQNPFPPPHAPTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN 60
           MEDI QN  PPP    P   D PP+FRCPISME+M DPVTISTGVSFERANIEKWFFTYN
Sbjct: 1   MEDIRQNRLPPPQ--PPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYN 60

Query: 61  KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLP-SSSMEYHDVVADLLK 120
           KKTCPATMQ IVNFDITPNYTLKRLILSWKM ++SCS  SS  P   S+EY DVVA LLK
Sbjct: 61  KKTCPATMQIIVNFDITPNYTLKRLILSWKM-NESCSFASSSSPRPPSVEYDDVVA-LLK 120

Query: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANF 180
           NIE SPFKASSLKKLRLLMA+DDVAAV AK +FV+ NGFEVL+G+VTQVAICESS+FANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKG 240
           EACEEALGVLSQFPFSKTEK FELLS+ EAIKSM IVLQRGSIEGRFYAME+LQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKG 240

Query: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEI 300
           YD+NSL+KH  IDLFKSILELASDEIIKKAS IKTTDSSI T CRYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIA 360
           MESSKK RLL IEAGA+C+MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGR-SSTK 420
           TVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMMGCGTVKKL+GIL NGDGR SSTK
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGIL-NGDGRSSSTK 420

Query: 421 EKAKKVMKLHGSFWRRFPCFPYEYRDYLKLIN 451
           EKA K+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 447

BLAST of CsaV3_6G006500 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 186.4 bits (472), Expect = 5.0e-47
Identity = 133/427 (31.15%), Postives = 212/427 (49.65%), Query Frame = 0

Query: 20  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 79
           ++ PP F CPIS+E+M+DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 80  YTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKNIESSPFKASSLKKLRLLMA 139
           +TL+RLI SW   + S        P   +   ++   +  +  S   +   LK+LR +++
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVS 129

Query: 140 VDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFEACEEALGVLSQFPFSKT-- 199
            +       K         E L  IV       S+D  N    +EAL +L     S+T  
Sbjct: 130 EN----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSETVL 189

Query: 200 EKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHQIIDLFKSI 259
           +       +   +KS+  ++QRG  E R YA  +L+ I +      S+T     ++F  +
Sbjct: 190 KNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVFTEV 249

Query: 260 LELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEIMESSKKSRLLSIEAGAVC 319
           +++  D I +KA+  K     +V  C +G                  ++R  ++EAG + 
Sbjct: 250 VQILDDRISQKAT--KAAMHILVNICPWG------------------RNRHKAVEAGVIS 309

Query: 320 VMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK-MSISNTAT 379
           V+I+LL D    S R   E  + +L LL +CAEGR+    H   IA V KK + +S TA+
Sbjct: 310 VIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTAS 369

Query: 380 KIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEKAKKVMKLHGSFW 439
              +++L  +  +  +  +L EM+  G V KL  +L    G   TKEKAK+++KLH   W
Sbjct: 370 DRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCG-GKTKEKAKELLKLHARVW 402

BLAST of CsaV3_6G006500 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 170.6 bits (431), Expect = 2.8e-42
Identity = 125/450 (27.78%), Postives = 225/450 (50.00%), Query Frame = 0

Query: 20  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 79
           ++ P  F CPIS+++M+DPV +STG++++R +IEKW F+  K +CP T Q I   D+TPN
Sbjct: 5   IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPN 64

Query: 80  YTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKNIESSPFKASSLKKLRLLMA 139
           +TL+RLI SW   + S        P   +   ++   + ++  S   +   LK+LR +++
Sbjct: 65  HTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVS 124

Query: 140 VD-------DVAAVGAKPVFVRVNGFEVLIGIVTQVA------ICESSDFAN-FEA---- 199
            +       + A V      +  N  +      + ++      +C+S+   N F++    
Sbjct: 125 ENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSL 184

Query: 200 CEEALGVLSQFPFSKTEKPFELLSE--PEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKG 259
            +EAL VL     S+T     L ++     +K++  ++QRG  E R YA  +L+++ +  
Sbjct: 185 MDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVA 244

Query: 260 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEI 319
                +   +  +LF  ++++  D+I  KA+  ++    +V  C +G             
Sbjct: 245 DPMQIILLER--ELFGEVIQILHDQISHKAT--RSAMQILVITCPWG------------- 304

Query: 320 MESSKKSRLLSIEAGAVCVMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGEHI 379
                ++R  ++E G + ++I+LL D    S R   E  + +L +L +CAEGR+    H 
Sbjct: 305 -----RNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHG 364

Query: 380 MGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGR 439
             IA V+KK + +S   ++  +++L  +  +  +  +L+EM+  G V KL  +L    G 
Sbjct: 365 AAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCG- 424

Query: 440 SSTKEKAKKVMKLHGSFWRRFPCFPYEYRD 445
           + TKEKAK+++KLH   WR  PC P    D
Sbjct: 425 NKTKEKAKELLKLHARVWRESPCVPRNLYD 431

BLAST of CsaV3_6G006500 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 143.7 bits (361), Expect = 3.7e-34
Identity = 116/441 (26.30%), Postives = 214/441 (48.53%), Query Frame = 0

Query: 20  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTI-VNFDITP 79
           ++ P +F CPIS+E+M+DPVT  +G++++R NI KW       +CP T Q + ++ D+TP
Sbjct: 22  IEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTP 81

Query: 80  NYTLKRLILSWKMKDQS---CSSTSSLLPSSSMEYHDVVADLLKNIESSPFKASSLKKLR 139
           N+ L+RLI  W +++++      ++  +P   +   + + +L K  + +  +  +L+KL 
Sbjct: 82  NHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLE 141

Query: 140 LLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFEACEEALGVLSQFPFS 199
           +L A+D       + +       + LI  V +    +       +  +E+L +L      
Sbjct: 142 VL-AMDG----NNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIP 201

Query: 200 KTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHQIIDLFK 259
             +    L+     ++S+  VL +     + Y + +L+ +++  Y  + + +    ++FK
Sbjct: 202 SNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFK 261

Query: 260 SILELASDEI----------------IKKASAIKTTDSSIVTHCRYGTLFSSALELLIEI 319
            I+    D +                  + S  KT  S +          ++AL +L+E 
Sbjct: 262 GIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLE- 321

Query: 320 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSK--CEKILHILKLLSECAEGRSAMGEHIMG 379
             S  ++R L ++ GAV  +I+L       K   E +L +L  L  CA GR+ +  H  G
Sbjct: 322 TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGG 381

Query: 380 IATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSS 438
           IA VTK+ + +S  A    + IL  +  + P   V+EEM+  GTV+KL  +L    G  +
Sbjct: 382 IAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG-LN 441

BLAST of CsaV3_6G006500 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 142.9 bits (359), Expect = 6.3e-34
Identity = 124/434 (28.57%), Postives = 203/434 (46.77%), Query Frame = 0

Query: 15  PTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNF 74
           P    +  P HFRCPIS+ELMQDPVT+ TG +++RA+IE W    N  TCP T   + +F
Sbjct: 7   PLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDF 66

Query: 75  DITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLK------NIESSPFKA 134
            + PN+TL+RLI  W + ++S        P    +   V A L +         S   +A
Sbjct: 67  TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRA 126

Query: 135 SSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFEACEEALGV 194
           ++L++LR      D   V    +    N  E+LI I+        S+  + E   E+L +
Sbjct: 127 AALRRLRGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELVSESLAL 186

Query: 195 LSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQIS--KKGYDWNSLT 254
           L   P ++  +   + S+P  ++ +  +L   SIE R  A  +++ +S   K  D     
Sbjct: 187 LVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSI 246

Query: 255 KHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEIMESSKKS 314
            +    +F+ +L+L  + I  +  A+K    ++   C                  S K +
Sbjct: 247 SNS-ESVFEGVLDLLRNPISSR-RALKIGIKTLFALC------------------SVKST 306

Query: 315 RLLSIEAGAVCVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK- 374
           R ++I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K  
Sbjct: 307 RHIAITAGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTI 366

Query: 375 MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEKAKKV 434
           + +S+ AT+     L  +C     ER  EE  G G V +LL ++   +     K+KA+K+
Sbjct: 367 LRVSDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLL-LMVQSECTERAKKKAQKL 406

Query: 435 MKLHGSFWRRFPCF 439
           +KL    W  +  F
Sbjct: 427 LKLLRDSWPDYNSF 406

BLAST of CsaV3_6G006500 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 123.6 bits (309), Expect = 4.0e-28
Identity = 121/430 (28.14%), Postives = 201/430 (46.74%), Query Frame = 0

Query: 15  PTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNF 74
           P    +  P HFRCPIS++LM DPVTISTG +++R +I+ W    N  TCP T   + +F
Sbjct: 7   PLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVALSDF 66

Query: 75  DITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDV-VADLLKNIE-------SSPF 134
            + PN+TL+RLI  W + ++  S+    +P+       + V  LL           S   
Sbjct: 67  TLIPNHTLRRLIQEWCVANR--SNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRS 126

Query: 135 KASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFEACEEAL 194
           +A+++++LR L    +   V    +    N  E+L+ I+   A  E++  ++ E   E+L
Sbjct: 127 RAAAIRRLRGLARDSEKNRV----LIAGHNAREILVRIL--FADIETTSLSS-ELVSESL 186

Query: 195 GVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQI--SKKGYDWNS 254
            +L     ++TE    + S+P  +  M  +L   SIE R  A  +++ +    K  D   
Sbjct: 187 ALLVLLHMTETECE-AVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKL 246

Query: 255 LTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEIMESSK 314
           +       +F+ +L+L  + I  +  A+K    +I   C                    K
Sbjct: 247 IISGS-DSIFEGVLDLLKNPISSR-RALKIGIKAIFALCLV------------------K 306

Query: 315 KSRLLSIEAGAVCVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTK 374
           ++R L+I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K
Sbjct: 307 QTRHLAISAGAPGILIDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVK 366

Query: 375 K-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTKEKAK 433
             + +S+ AT+     L  +C     ER  +E    G V +LL +L   D     K KA+
Sbjct: 367 TILRVSDRATEYAAGALLALCT--AEERCRDEAAAAGLVTQLL-LLVQSDCTERAKRKAQ 402

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004145072.25.2e-256100.00E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN46274.1 hypothetical pro... [more]
XP_008460073.12.7e-23693.83PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo][more]
XP_038875803.13.4e-23191.61E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida][more]
XP_023549278.11.1e-21386.34E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo][more]
XP_022958929.12.7e-21285.24E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q84TG37.0e-4631.15E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC64.0e-4127.78E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q9SF155.2e-3326.30E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9LT798.9e-3328.57U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9FXA45.6e-2728.14U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0KBP62.5e-256100.00RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE... [more]
A0A1S3CC541.3e-23693.83RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 ... [more]
A0A6J1H6I81.3e-21285.24RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075... [more]
A0A5D3DLY22.5e-21186.12RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1KWQ83.0e-20985.18RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 P... [more]
Match NameE-valueIdentityDescription
AT2G35930.15.0e-4731.15plant U-box 23 [more]
AT3G52450.12.8e-4227.78plant U-box 22 [more]
AT3G11840.13.7e-3426.30plant U-box 24 [more]
AT3G19380.16.3e-3428.57plant U-box 25 [more]
AT1G49780.14.0e-2828.14plant U-box 26 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 25..89
e-value: 8.6E-24
score: 95.0
IPR003613U box domainPFAMPF04564U-boxcoord: 23..93
e-value: 5.3E-16
score: 58.6
IPR003613U box domainPROSITEPS51698U_BOXcoord: 21..96
score: 25.123554
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 99..429
e-value: 8.9E-10
score: 39.7
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 14..98
e-value: 1.5E-23
score: 84.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR22849:SF23RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 21..450
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 21..450
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 26..69
e-value: 5.70851E-21
score: 83.437
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 19..92
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 110..414

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_6G006500.1CsaV3_6G006500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity