CsaV3_6G000540 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_6G000540
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionFe2OG dioxygenase domain-containing protein
Locationchr6: 360508 .. 364230 (-)
RNA-Seq ExpressionCsaV3_6G000540
SyntenyCsaV3_6G000540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATATTTTAACATGGGTAATAAATTAGTTTTCAAGGCAAAAACTATCATTATACATGAAAAATATTTTTCACATATAGTGAAATTTTTATTTTTGTTCATATTGTATAGATAAAATGATTATGGACCAAATGACTTTAATCAAGTAGGCTCAAGTCAAACAAAGCCAAACAGGGAAGCATTAGATATGACTGTGAGTAGATAAAGTGTTAACTCTATTATACACAAAGCTTAAAGAGGCTTGTTTTGGTGGATTGTCCATTTATGGGAATTACCCAAAACAATCCATGGAGAAGGCTAACTATCATGTCTGTCATTCTACTTCTACTCCTGATGATTAGTTCAAAGTTTCAGTTCCCAAAGACACTCAAACAAATTTCCTTTCAACTTTTTCTTAACCACAATCTGAGAATTCAAACCAATTTACTTTTCTTACTTACTCCCTTGTTTTTCACTTTGGAGTTATTTGTATAGAAGCCCTCCAAAAACAATGTCCCAAATAGTTCTCGTCTCAATTTTTGAAATAGAAATTTTTTGCCTTTTTTACTAACATCGTCATCAATCAACATTAACAAAATAGCCTTAATCCTTCTTTTGCCCTCTTCTTCTGATTGATTTTCTTCTCTTCTCCACTTTTTTTTTTCACCTGTACTGCAGAATGGTAGTTGGCTGTGTATTCACGGGAGAGGTTATGGACTCGCCGAAAAGGGATTTGAGAGATCATCTTAGCGTCGGATGTGTGGGACTTTGTCTGAACATAGATTTGTTGGGTGGATTGATTTTTTTCCTCTAGCTAGATTTGTGGTTTTTCATCGAAAAGTGGTGTTGGAGAAAGAGCAAGGGACGTGTGTTTGGGACATATGATGTACGGGTTTTCCTCATAAAGTGGAGAGAGGGAAGACCGACAGAGAGAACTAGAGAATATTAGAGCAATTAAGATCCTAATCCATGTGCAAAATGGTTCGTTTAGAAATGGGAAGATTTCATCTCGTCTTAAGATTATGGTTGGTGGAGATCCATCAGTATGAAGTGAACAAATAGTATTAAGCCTAGTAATTCATTAATCCTCTGGTTGATGGTAAGAACTAACAAGTGTAACTAAGTTGGGTTGGTTGAACCAATATGTCAGTCCAACAAAATAAAAGACCCAAGCCTAACTTCTTAAAGACAAACACAAATTGACCATAACCAACTAAAAAAAAACCAAAATCAATCAAACCAACAATGGCAATAAGAAAGTGAACCAAACAACAATAATCAACCTATTGCAATTGAACTAACGCCACGAGACCAAGTTGAGCTCGAGAAGTTTTAATGACTCAAGGTTCATATTAGAAAAAGGAATTCCATCCTGATTATCTTATTTGCTCCAACAAACTAGCATGGTTCTATAATTAAAGCAAATTGGTCAACTTTTCCATGTCTAGACCGTGTGTGAGATACAACACAACTTAAGACTTAATCTAAAATATGCAATTGTTTATAACAGTGTAAGAAAAGTCAAGACTTAATCTAGTAGGCAATGGCAACTAAAGATGCTTTTCAGATGAGGATACCATTTGACCTCAACATATATAATTTTTTAAAGAACAAACATCAACACACCAGTATTTTCATTTAAAATTACAGCTAAATATTCTTCTAAATGTGAAGCTAGACCAAACTTTATGGGCTGAAATTGAGATATTCAAACAATGATTACATTCAATCTTTTTCTAACCTCTACATGCTTGTTAACAAAGTATTTGGATTGGTCAAACCTCTATCAAATAGTATATCCAAACCCTATAGAACCCAATTAGCACAGAATGACAGATATTATATATATATATATACAAAAGTCTGCAGCAGAAAGTTTCCACCTTACCAGCCTGTCTTGGCTTCATGTAAATGCAAAGAGCTTGGAATTATTTAAGCTTCCTACAATACCTGCTTGCTCCTCATAACAAACAAAAACTTATAATATTTCTAAGCATGTCAAGTCAAACCCTTTCACTAATTACAATTCTGAATCAGCTACCCACTTTTCAAATCACTGTATTATTGTTTTAATTGTTTTCATGGTTGCTTTTCCAAGTAACAGAAATCAACCCACTAATGATTGAAACTAAAGACAATAAAGTAGAAGTTTTGACTATATGCATCTTTTTTTTTTTAGTTAAACAATTATAAGGTAGGGTAGGGTAGGCGAATGGAATCATTCACGTTTGAAATGACAATTGATGTCTTATCTTCTAAACTATTCTCAATTGATGTCTTATCTACTAAACTATACTCAGATTGGTATTTGCATCTTTCGTTTTAAGGAATATAATTATCCACCTATGAAGTATAGGCTAGCTAGCTATAGACAAACATGTGTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCTTTGTCTCCGTCTTCCCTTTCTTCACTAGCTGATGCTTGCAAAAAATGGGGGTTTTTCCACATAAAAAACCATGGGATCTCCAAAGAAATCTACAACAAACTCCATTCCTTCTCCAATGAAATTTTCAACCTCCCTTCAGAAACCAAGCTCAAAATTGGCCCCCTCTCTTCCGTAAACACTTACACTCCCCATTTCATAGCCTCCCCATTCTTTGAAACCCTCAGAGTCTCTGGTCCAAACTTCTTAGCCTCTGCTCAGAATTCAGCAGAGTTCCTCTTTAACCAAAAGAGCTCTCACCAGTTCAGGTACACACAATTCACATCACAGCACATCCACATCCACATCCACTTCAGAATCACTATCTCTGTAATCTAAGATCATTACTTCGTTTATCCTAACAATCTACTTTCATGTGGTTAAAGTGAGATATTGCAAGAATATGGAGATAAAATGACAGAGCTATCAAGGAAAATTATCAAGAACATACTGATGAGCTTTGGTGAAGGTTTTGAGAAGAAATACCATGAACCCCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAACTACACACCTCCAGAAACGTTAGAAGACGATGAAGAAGTAGAAGGACTTGGAATGCATACAGATATGAGCTGCGTGACCATTGTGTACCAAGATCAAACAGGTGGTCTGCAAGTGAAATCAAGCGAAGGGAAATGGGTGGACATAAACCCATGTGAAGAATCAGAAGCACTGGTGGTGAATATTGGGGACTTGCTTCAAGCTTGGAGCAACGACAGGGTGAGGTCGTCGGAGCACAGAGTGGTTTTGAGGCGGCCGGCGATCAACCGATTCTCGATTGCTTTCTTTTGGGGTTTTGAGGACGAGAAGGTGGTGTCAGCGCCGGAGGACTTGGTGGAAGAGGGCGGTGAGAGGATGTATAAGCCATTTGTGTGCTCGGATTATTTGAAGTTCAGGGAGAATGATGAAAGAGGGAAATTTGAAAAGGTTGGTTTCACCGTCACAAACTTTGCTGGGAAAAAGGATTAATACGTTAATTAATTATGTTTTTTTTTTGTATAACTACTTTTGCAAATTTCATTTTCCAATTTAAGGAATTCTTATAAATAAAAAAAATGAAGCAATTAAATTTATATAGCAAAAAACTTTTTAAATCAACCAGAGAATTTTGAAATAGGTTTAATATTTTGCTTAATTTAATTATAATGACTTTGTGAAAGATTAATTAATGTTTATCAAACTTTATTTTTATTTATTTATCATCTGTTTTAAGCGAAATAAAATTATTATTCATACGTACATTTTAAA

mRNA sequence

ATGTGTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCTTTGTCTCCGTCTTCCCTTTCTTCACTAGCTGATGCTTGCAAAAAATGGGGGTTTTTCCACATAAAAAACCATGGGATCTCCAAAGAAATCTACAACAAACTCCATTCCTTCTCCAATGAAATTTTCAACCTCCCTTCAGAAACCAAGCTCAAAATTGGCCCCCTCTCTTCCGTAAACACTTACACTCCCCATTTCATAGCCTCCCCATTCTTTGAAACCCTCAGAGTCTCTGGTCCAAACTTCTTAGCCTCTGCTCAGAATTCAGCAGAGTTCCTCTTTAACCAAAAGAGCTCTCACCAGTTCAGTGAGATATTGCAAGAATATGGAGATAAAATGACAGAGCTATCAAGGAAAATTATCAAGAACATACTGATGAGCTTTGGTGAAGGTTTTGAGAAGAAATACCATGAACCCCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAACTACACACCTCCAGAAACGTTAGAAGACGATGAAGAAGTAGAAGGACTTGGAATGCATACAGATATGAGCTGCGTGACCATTGTGTACCAAGATCAAACAGGTGGTCTGCAAGTGAAATCAAGCGAAGGGAAATGGGTGGACATAAACCCATGTGAAGAATCAGAAGCACTGGTGGTGAATATTGGGGACTTGCTTCAAGCTTGGAGCAACGACAGGGTGAGGTCGTCGGAGCACAGAGTGGTTTTGAGGCGGCCGGCGATCAACCGATTCTCGATTGCTTTCTTTTGGGGTTTTGAGGACGAGAAGGTGGTGTCAGCGCCGGAGGACTTGGTGGAAGAGGGCGGTGAGAGGATGTATAAGCCATTTGTGTGCTCGGATTATTTGAAGTTCAGGGAGAATGATGAAAGAGGGAAATTTGAAAAGGTTGGTTTCACCGTCACAAACTTTGCTGGGAAAAAGGATTAA

Coding sequence (CDS)

ATGTGTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCTTTGTCTCCGTCTTCCCTTTCTTCACTAGCTGATGCTTGCAAAAAATGGGGGTTTTTCCACATAAAAAACCATGGGATCTCCAAAGAAATCTACAACAAACTCCATTCCTTCTCCAATGAAATTTTCAACCTCCCTTCAGAAACCAAGCTCAAAATTGGCCCCCTCTCTTCCGTAAACACTTACACTCCCCATTTCATAGCCTCCCCATTCTTTGAAACCCTCAGAGTCTCTGGTCCAAACTTCTTAGCCTCTGCTCAGAATTCAGCAGAGTTCCTCTTTAACCAAAAGAGCTCTCACCAGTTCAGTGAGATATTGCAAGAATATGGAGATAAAATGACAGAGCTATCAAGGAAAATTATCAAGAACATACTGATGAGCTTTGGTGAAGGTTTTGAGAAGAAATACCATGAACCCCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAACTACACACCTCCAGAAACGTTAGAAGACGATGAAGAAGTAGAAGGACTTGGAATGCATACAGATATGAGCTGCGTGACCATTGTGTACCAAGATCAAACAGGTGGTCTGCAAGTGAAATCAAGCGAAGGGAAATGGGTGGACATAAACCCATGTGAAGAATCAGAAGCACTGGTGGTGAATATTGGGGACTTGCTTCAAGCTTGGAGCAACGACAGGGTGAGGTCGTCGGAGCACAGAGTGGTTTTGAGGCGGCCGGCGATCAACCGATTCTCGATTGCTTTCTTTTGGGGTTTTGAGGACGAGAAGGTGGTGTCAGCGCCGGAGGACTTGGTGGAAGAGGGCGGTGAGAGGATGTATAAGCCATTTGTGTGCTCGGATTATTTGAAGTTCAGGGAGAATGATGAAAGAGGGAAATTTGAAAAGGTTGGTTTCACCGTCACAAACTTTGCTGGGAAAAAGGATTAA

Protein sequence

MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFSEILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDEEVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLKFRENDERGKFEKVGFTVTNFAGKKD*
Homology
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BLAST of CsaV3_6G000540 vs. NCBI nr
Match: XP_004138503.2 (gibberellin 20-oxidase-like protein [Cucumis sativus] >KGN45657.1 hypothetical protein Csa_005484 [Cucumis sativus])

HSP 1 Score: 664.5 bits (1713), Expect = 4.8e-187
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0 

Query: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60
           MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF
Sbjct: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60

Query: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120
           NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS
Sbjct: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180
           EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240
           EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND
Sbjct: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240

Query: 241 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLKFR 300
           RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLKFR
Sbjct: 241 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLKFR 300

Query: 301 ENDERGKFEKVGFTVTNFAGKKD 324
           ENDERGKFEKVGFTVTNFAGKKD
Sbjct: 301 ENDERGKFEKVGFTVTNFAGKKD 323

[Close]
BLAST of CsaV3_6G000540 vs. NCBI nr
Match: XP_008458231.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo] >TYK03012.1 putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa])

HSP 1 Score: 632.1 bits (1629), Expect = 2.6e-177
Identity = 305/321 (95.02%), Postives = 316/321 (98.44%), Query Frame = 0 

Query: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60
           M VSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIY+KLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYDKLHSFSNEIF 60

Query: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120
           +LP ETKLKIGPLSS+NTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS
Sbjct: 61  SLPLETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180
           EILQEYG+KMTELSRKIIKN+L+SFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE
Sbjct: 121 EILQEYGEKMTELSRKIIKNVLLSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND
Sbjct: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240

Query: 241 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLKFR 300
           R+RSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP++LV EGG+RMYKPFVCSDYLKFR
Sbjct: 241 RLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPKELVGEGGKRMYKPFVCSDYLKFR 300

Query: 301 ENDERGKFEKVGFTVTNFAGK 322
           ENDERGKFEKVGFTV +FAGK
Sbjct: 301 ENDERGKFEKVGFTVRDFAGK 321

[Close]
BLAST of CsaV3_6G000540 vs. NCBI nr
Match: XP_038875412.1 (gibberellin 20-oxidase-like protein [Benincasa hispida])

HSP 1 Score: 604.7 bits (1558), Expect = 4.5e-169
Identity = 298/334 (89.22%), Postives = 311/334 (93.11%), Query Frame = 0 

Query: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60
           M VS ASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISK+ YNKLHSFSNEIF
Sbjct: 1   MSVSNASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60

Query: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120
           +LP ETKLKIGPLSS+NTYTPHFIASPFFETLRVSGPNFLASAQ+SA+ LFNQKS HQFS
Sbjct: 61  SLPIETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADLLFNQKSPHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180
           EILQEYG+KMTELSRKIIKNILMSFGEGFEK YHEPHFKNCHGYLRINNYT PETLE+DE
Sbjct: 121 EILQEYGEKMTELSRKIIKNILMSFGEGFEKTYHEPHFKNCHGYLRINNYTSPETLEEDE 180

Query: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCE--------ESEALVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDI+PCE        ESEALVVNIGD
Sbjct: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESELESKSESEALVVNIGD 240

Query: 241 LLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFV 300
           LLQAW NDR+RSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPE+LV +GGER+YKPFV
Sbjct: 241 LLQAWGNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVGDGGERLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVTNFAG---KKD 324
           CSDYLKFRENDERGKFEKVGFTV +FAG   KKD
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNGIKKD 334

[Close]
BLAST of CsaV3_6G000540 vs. NCBI nr
Match: KAG6575175.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 592.8 bits (1527), Expect = 1.8e-165
Identity = 290/328 (88.41%), Postives = 306/328 (93.29%), Query Frame = 0 

Query: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60
           M VSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISK+IYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120
           +LPSETKLKIGPLSS+NTYTPHFIASPFFETLRVSGPNFLASAQ+SA+ LFNQKS HQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180
           EILQEYGDKMTELSRKIIKNILMS GEGF+ KYHE HFKNCHGYLRINNYTPPETL +DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEE--------SEALVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+E        SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGD 240

Query: 241 LLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFV 300
           LLQAWSNDR+RSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPE++V +GG+R+YKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVTNFAG 321
           CSDYLKFRENDERGKFEKVGFTV +FAG
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAG 328

[Close]
BLAST of CsaV3_6G000540 vs. NCBI nr
Match: KAG7013737.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 592.8 bits (1527), Expect = 1.8e-165
Identity = 290/328 (88.41%), Postives = 306/328 (93.29%), Query Frame = 0 

Query: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60
           M VSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISK+IYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120
           +LPSETKLKIGPLSS+NTYTPHFIASPFFETLRVSGPNFLASAQ+SA+ LFNQKS HQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180
           EILQEYGDKMTELSRKIIKNILMS GEGF+ KYHE HFKNCHGYLRINNYTPPETL +DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEE--------SEALVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+E        SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGD 240

Query: 241 LLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFV 300
           LLQAWSNDR+RSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPE++V +GG+R+YKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVTNFAG 321
           CSDYLKFRENDERGKFEKVGFTV +FAG
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAG 328

[Close]
BLAST of CsaV3_6G000540 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 2.7e-100
Identity = 184/328 (56.10%), Postives = 242/328 (73.78%), Query Frame = 0 

Query: 7   SVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPSET 66
           S+ELP+ DIS+PLS SSL+SL DACK+WGFF++ NHG+S+++Y KL  FS  +F L  E 
Sbjct: 2   SLELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 61

Query: 67  KLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFSEILQEY 126
           K+K+G     + YTP FIASPFFE+LRVSGP+F ASA++S +   +Q +  +FS +++EY
Sbjct: 62  KMKMG----ASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEY 121

Query: 127 GDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDEE----- 186
           G+KMT+L  KI+K IL SFG+    KY+E  F NCHGY RINNYT P   EDD       
Sbjct: 122 GEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQ 181

Query: 187 --VEGLGMHTDMSCVTIVYQDQTGGLQVKSSEG-KWVDINPCEESEALVVNIGDLLQAWS 246
             +EGLGMHTDMSC+TIV QD  GGLQV++ +G   +DINP  + EALVVN+GDLL AW+
Sbjct: 182 DLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINP--KDEALVVNVGDLLHAWT 241

Query: 247 NDRVRSSEHRVVLRRPAI--NRFSIAFFWGFEDEKVVSAPEDLVEE-GGERMYKPFVCSD 306
           N R+RSS+HRV+L+R     NRFS+AFFW F+D KVV AP+++V    G R+++ F C D
Sbjct: 242 NGRLRSSQHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEGMRVFRSFKCGD 301

Query: 307 YLKFRENDERGKFEKVGFTVTNFAGKKD 324
           YL+FRE++E+GKFEKVG TV +FA  +D
Sbjct: 302 YLRFRESNEKGKFEKVGDTVEDFARIED 323

[Close]
BLAST of CsaV3_6G000540 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 1.6e-31
Identity = 96/316 (30.38%), Postives = 167/316 (52.85%), Query Frame = 0 

Query: 3   VSKASVELPILDISQPLS-PSSLSS----LADACKKWGFFHIKNHGISKEIYNKLHSFSN 62
           ++   +++P++D+   LS PSS       +++ACKK GFF + NHGIS+E+ +  H +++
Sbjct: 54  INVLELDVPLIDLQNLLSDPSSTLDASRLISEACKKHGFFLVVNHGISEELISDAHEYTS 113

Query: 63  EIFNLPSETKLKI-----GPLSSVNTYTPHFIAS-PFFETLRVSGPNFLASAQNSAEFLF 122
             F++P   K ++       +   +++T  F    P+ ETL     + ++ +++  ++  
Sbjct: 114 RFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCDDMSRSKSVQDYFC 173

Query: 123 NQ--KSSHQFSEILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINN 182
           +        F ++ QEY + M+ LS KI++  L+    G ++ Y    F+     +R+N 
Sbjct: 174 DALGHGFQPFGKVYQEYCEAMSSLSLKIME--LLGLSLGVKRDYFREFFEENDSIMRLNY 233

Query: 183 YTPPETLEDDEEVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVN 242
           Y  P  ++ D  + G G H D + +TI++QD   GLQV   E +W  I P    +A VVN
Sbjct: 234 Y--PPCIKPDLTL-GTGPHCDPTSLTILHQDHVNGLQV-FVENQWRSIRP--NPKAFVVN 293

Query: 243 IGDLLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYK 302
           IGD   A SNDR +S  HR V+   +  R S+AFF   + ++VV+ P +L++    R Y 
Sbjct: 294 IGDTFMALSNDRYKSCLHRAVVNSES-ERKSLAFFLCPKKDRVVTPPRELLDSITSRRYP 353

Query: 303 PFVCSDYLKFRENDER 306
            F  S +L+F +   R
Sbjct: 354 DFTWSMFLEFTQKHYR 360

[Close]
BLAST of CsaV3_6G000540 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 3.5e-31
Identity = 101/301 (33.55%), Postives = 148/301 (49.17%), Query Frame = 0 

Query: 10  LPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPSETKLK 69
           +P++D+S   +P   + + DA K WG F I NHGIS+++ + + S S  +F++PSE KL+
Sbjct: 49  IPVIDLS---NPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLFDMPSERKLE 108

Query: 70  IGPL-SSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSH---QFSEILQE 129
                  V+ Y    I SPFFE    S       A +S    FN    H   ++  I+QE
Sbjct: 109 AASSDKGVSGYGEPRI-SPFFEKKMWS--EGFTIADDSYRNHFNTLWPHDHTKYCGIIQE 168

Query: 130 YGDKMTELSRKIIKNILMSFGEGFE-----KKYHEPHFKNCHGYLRINNYTPPETLEDDE 189
           Y D+M +L+ +++  IL S G   E      K  +   K   G +R+N+Y       + E
Sbjct: 169 YVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHY---PVCPEPE 228

Query: 190 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 249
              GL  HTD + +TI++Q  TGGLQV   E  WV + P      LVVNIGDL    SN 
Sbjct: 229 RAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPA--PGVLVVNIGDLFHILSNG 288

Query: 250 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP-EDLVEEGGERMYKPFVCSDYLKF 301
           ++ S  HR  +     +R SIA+ WG     V  AP   L       +Y+     +YL+ 
Sbjct: 289 KIPSVVHRAKVNHTR-SRISIAYLWGGPAGDVQIAPISKLTGPAEPSLYRSITWKEYLQI 337

[Close]
BLAST of CsaV3_6G000540 vs. ExPASy Swiss-Prot
Match: Q9LHN8 (Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.6e-31
Identity = 94/308 (30.52%), Postives = 158/308 (51.30%), Query Frame = 0 

Query: 3   VSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNL 62
           V++    +P++D+S P       ++ DA +KWGFF + NHG+  E+ + + + +++ FNL
Sbjct: 55  VNETDEAIPVIDMSNPDEDRVAEAVCDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNL 114

Query: 63  PSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAE-------FLFNQKS 122
           P E K K    +S++T            T+R  G +F   A+ + E       F  ++  
Sbjct: 115 PVEEKRKFTKENSLST------------TVRF-GTSFSPLAEQALEWKDYLSLFFVSEAE 174

Query: 123 SHQF-SEILQEYGDKMTELSRKIIKNILMSFGEGFE-KKYHEPHFKNCHGYLRIN-NYTP 182
           + QF  +I +    +    S+K+++ +L   G+    K+  E       G +R+N NY P
Sbjct: 175 AEQFWPDICRNETLEYINKSKKMVRRLLEYLGKNLNVKELDETKESLFMGSIRVNLNYYP 234

Query: 183 PETLEDDEEVEGLGMHTDMSCVTIVYQDQTGGLQVKS-SEGKWVDINPCEESEALVVNIG 242
                + +   G+G H+D+S +TI+ QDQ GGL V+S + G WV + P   S   V+NIG
Sbjct: 235 --ICPNPDLTVGVGRHSDVSSLTILLQDQIGGLHVRSLASGNWVHVPPVAGS--FVINIG 294

Query: 243 DLLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPF 300
           D +Q  SN   +S EHR VL     NR S+  F   + E V+    +++  G E +Y+  
Sbjct: 295 DAMQIMSNGLYKSVEHR-VLANGYNNRISVPIFVNPKPESVIGPLPEVIANGEEPIYRDV 344

[Close]
BLAST of CsaV3_6G000540 vs. ExPASy Swiss-Prot
Match: Q9C899 (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.0e-30
Identity = 95/299 (31.77%), Postives = 153/299 (51.17%), Query Frame = 0 

Query: 10  LPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPSETKLK 69
           +P++DIS     S   ++ DA ++WGFF + NHG+S E+   + + ++  F LP E K K
Sbjct: 62  IPVIDISNLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPVEEKRK 121

Query: 70  IGPLSSVNT-------YTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFSEI 129
                S++T       ++PH  A    E        F++ A+  A  L+      +  E 
Sbjct: 122 FSREKSLSTNVRFGTSFSPH--AEKALEWKDYLSLFFVSEAE--ASQLWPDSCRSETLEY 181

Query: 130 LQEYGDKMTELSRKIIKNILMSFGEGFE-KKYHEPHFKNCHGYLRIN-NYTPPETLEDDE 189
           + E        ++ ++K +L   GE    K+  +       G  RIN NY P     + E
Sbjct: 182 MNE--------TKPLVKKLLRFLGENLNVKELDKTKESFFMGSTRINLNYYP--ICPNPE 241

Query: 190 EVEGLGMHTDMSCVTIVYQDQTGGLQVKS-SEGKWVDINPCEESEALVVNIGDLLQAWSN 249
              G+G H+D+S +TI+ QD+ GGL V+S + G+WV + P   S +LV+NIGD +Q  SN
Sbjct: 242 LTVGVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPI--SGSLVINIGDAMQIMSN 301

Query: 250 DRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLK 299
            R +S EHR VL   + NR S+  F   + E V+    +++E G + +YK  + +DY+K
Sbjct: 302 GRYKSVEHR-VLANGSYNRISVPIFVSPKPESVIGPLLEVIENGEKPVYKDILYTDYVK 343

[Close]
BLAST of CsaV3_6G000540 vs. ExPASy TrEMBL
Match: A0A0A0KB92 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G004480 PE=3 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 2.3e-187
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0 

Query: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60
           MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF
Sbjct: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60

Query: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120
           NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS
Sbjct: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180
           EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240
           EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND
Sbjct: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240

Query: 241 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLKFR 300
           RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLKFR
Sbjct: 241 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLKFR 300

Query: 301 ENDERGKFEKVGFTVTNFAGKKD 324
           ENDERGKFEKVGFTVTNFAGKKD
Sbjct: 301 ENDERGKFEKVGFTVTNFAGKKD 323

[Close]
BLAST of CsaV3_6G000540 vs. ExPASy TrEMBL
Match: A0A5D3BVZ1 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001070 PE=3 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.3e-177
Identity = 305/321 (95.02%), Postives = 316/321 (98.44%), Query Frame = 0 

Query: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60
           M VSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIY+KLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYDKLHSFSNEIF 60

Query: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120
           +LP ETKLKIGPLSS+NTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS
Sbjct: 61  SLPLETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180
           EILQEYG+KMTELSRKIIKN+L+SFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE
Sbjct: 121 EILQEYGEKMTELSRKIIKNVLLSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND
Sbjct: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240

Query: 241 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLKFR 300
           R+RSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP++LV EGG+RMYKPFVCSDYLKFR
Sbjct: 241 RLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPKELVGEGGKRMYKPFVCSDYLKFR 300

Query: 301 ENDERGKFEKVGFTVTNFAGK 322
           ENDERGKFEKVGFTV +FAGK
Sbjct: 301 ENDERGKFEKVGFTVRDFAGK 321

[Close]
BLAST of CsaV3_6G000540 vs. ExPASy TrEMBL
Match: A0A1S3C8M6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LOC103497717 PE=3 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.3e-177
Identity = 305/321 (95.02%), Postives = 316/321 (98.44%), Query Frame = 0 

Query: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60
           M VSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIY+KLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYDKLHSFSNEIF 60

Query: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120
           +LP ETKLKIGPLSS+NTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS
Sbjct: 61  SLPLETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180
           EILQEYG+KMTELSRKIIKN+L+SFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE
Sbjct: 121 EILQEYGEKMTELSRKIIKNVLLSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND
Sbjct: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240

Query: 241 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLKFR 300
           R+RSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP++LV EGG+RMYKPFVCSDYLKFR
Sbjct: 241 RLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPKELVGEGGKRMYKPFVCSDYLKFR 300

Query: 301 ENDERGKFEKVGFTVTNFAGK 322
           ENDERGKFEKVGFTV +FAGK
Sbjct: 301 ENDERGKFEKVGFTVRDFAGK 321

[Close]
BLAST of CsaV3_6G000540 vs. ExPASy TrEMBL
Match: A0A6J1H632 (gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111460359 PE=3 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 8.6e-166
Identity = 290/328 (88.41%), Postives = 306/328 (93.29%), Query Frame = 0 

Query: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60
           M VSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISK+IYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120
           +LPSETKLKIGPLSS+NTYTPHFIASPFFETLRVSGPNFLASAQ+SA+ LFNQKS HQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180
           EILQEYGDKMTELSRKIIKNILMS GEGF+ KYHE HFKNCHGYLRINNYTPPETL +DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEE--------SEALVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+E        SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGD 240

Query: 241 LLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFV 300
           LLQAWSNDR+RSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPE++V +GG+R+YKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVTNFAG 321
           CSDYLKFRENDERGKFEKVGFTV +FAG
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAG 328

[Close]
BLAST of CsaV3_6G000540 vs. ExPASy TrEMBL
Match: A0A6J1KY83 (gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111499287 PE=3 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.1e-165
Identity = 287/322 (89.13%), Postives = 306/322 (95.03%), Query Frame = 0 

Query: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60
           M VSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISK+IYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120
           +LPSETKLKIGPLSS+NTYTPHFIASPFFETLRVSGPNFLASAQ+SA+FLFNQKS HQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180
           EILQ+YGDKMTELSRKIIKNILMS GEGF+  YHE HFKNCHGYLRINNYTPPETL +DE
Sbjct: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCE--ESEALVVNIGDLLQAWS 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+  ESEALVVNIGDLLQAWS
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESEALVVNIGDLLQAWS 240

Query: 241 NDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLK 300
           ND++RSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPE++V +GG+++YKPFVCSDYLK
Sbjct: 241 NDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDYLK 300

Query: 301 FRENDERGKFEKVGFTVTNFAG 321
           FRENDERGKFEKVGFTV +FAG
Sbjct: 301 FRENDERGKFEKVGFTVRDFAG 322

[Close]
BLAST of CsaV3_6G000540 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 392.1 bits (1006), Expect = 4.3e-109
Identity = 189/319 (59.25%), Postives = 251/319 (78.68%), Query Frame = 0 

Query: 6   ASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPSE 65
           +S++LP+LD++QP+  S LSSL++ACK+WGFF++ NHGISKE+++K+ S S ++F  P E
Sbjct: 6   SSLQLPVLDLTQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVFKAPLE 65

Query: 66  TKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFSEILQE 125
           +KLK+GP+S    YTP +IASP+FE+L VSGP+F  SA+ SA+ LF      +  E +QE
Sbjct: 66  SKLKLGPIS----YTPRYIASPYFESLVVSGPDFSDSAKASADVLFQDHHKPELRETMQE 125

Query: 126 YGDKMTELSRKIIKNIL-MSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDEE-VE 185
           YG KM ELS+++IK +L M+ G+   K+ ++  F NCHGYLR+ NYTPP  +E  EE VE
Sbjct: 126 YGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEELVE 185

Query: 186 GLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSNDRVR 245
           GLGMHTDMSC+TIVYQD  GGLQ++S EGKW+DINPC  ++ LVVNIGDL+QAWSN R+R
Sbjct: 186 GLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPC--NDFLVVNIGDLMQAWSNGRLR 245

Query: 246 SSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLKFREND 305
           SSEHRVVLR+  +NR S+AFF  FEDEKV+ AP+++V EG +R YK F CS+YLKFR+++
Sbjct: 246 SSEHRVVLRK-LVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQSN 305

Query: 306 ERGKFEKVGFTVTNFAGKK 323
           E GKFEK+GFTV +FAG K
Sbjct: 306 EEGKFEKIGFTVKDFAGLK 317

[Close]
BLAST of CsaV3_6G000540 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 366.7 bits (940), Expect = 1.9e-101
Identity = 184/328 (56.10%), Postives = 242/328 (73.78%), Query Frame = 0 

Query: 7   SVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPSET 66
           S+ELP+ DIS+PLS SSL+SL DACK+WGFF++ NHG+S+++Y KL  FS  +F L  E 
Sbjct: 2   SLELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 61

Query: 67  KLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFSEILQEY 126
           K+K+G     + YTP FIASPFFE+LRVSGP+F ASA++S +   +Q +  +FS +++EY
Sbjct: 62  KMKMG----ASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEY 121

Query: 127 GDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDEE----- 186
           G+KMT+L  KI+K IL SFG+    KY+E  F NCHGY RINNYT P   EDD       
Sbjct: 122 GEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQ 181

Query: 187 --VEGLGMHTDMSCVTIVYQDQTGGLQVKSSEG-KWVDINPCEESEALVVNIGDLLQAWS 246
             +EGLGMHTDMSC+TIV QD  GGLQV++ +G   +DINP  + EALVVN+GDLL AW+
Sbjct: 182 DLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINP--KDEALVVNVGDLLHAWT 241

Query: 247 NDRVRSSEHRVVLRRPAI--NRFSIAFFWGFEDEKVVSAPEDLVEE-GGERMYKPFVCSD 306
           N R+RSS+HRV+L+R     NRFS+AFFW F+D KVV AP+++V    G R+++ F C D
Sbjct: 242 NGRLRSSQHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEGMRVFRSFKCGD 301

Query: 307 YLKFRENDERGKFEKVGFTVTNFAGKKD 324
           YL+FRE++E+GKFEKVG TV +FA  +D
Sbjct: 302 YLRFRESNEKGKFEKVGDTVEDFARIED 323

[Close]
BLAST of CsaV3_6G000540 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 269.2 bits (687), Expect = 4.2e-72
Identity = 129/202 (63.86%), Postives = 163/202 (80.69%), Query Frame = 0 

Query: 123 LQEYGDKMTELSRKIIKNIL-MSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDEE 182
           +QEYG KM ELS+++IK +L M+ G+   K+ ++  F NCHGYLR+ NYTPP  +E  EE
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 60

Query: 183 -VEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 242
            VEGLGMHTDMSC+TIVYQD  GGLQ++S EGKW+DINPC  ++ LVVNIGDL+QAWSN 
Sbjct: 61  LVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPC--NDFLVVNIGDLMQAWSNG 120

Query: 243 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLKFR 302
           R+RSSEHRVVLR+  +NR S+AFF  FEDEKV+ AP+++V EG +R YK F CS+YLKFR
Sbjct: 121 RLRSSEHRVVLRK-LVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFR 180

Query: 303 ENDERGKFEKVGFTVTNFAGKK 323
           +++E GKFEK+GFTV +FAG K
Sbjct: 181 QSNEEGKFEKIGFTVKDFAGLK 199

[Close]
BLAST of CsaV3_6G000540 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 138.3 bits (347), Expect = 1.1e-32
Identity = 96/316 (30.38%), Postives = 167/316 (52.85%), Query Frame = 0 

Query: 3   VSKASVELPILDISQPLS-PSSLSS----LADACKKWGFFHIKNHGISKEIYNKLHSFSN 62
           ++   +++P++D+   LS PSS       +++ACKK GFF + NHGIS+E+ +  H +++
Sbjct: 54  INVLELDVPLIDLQNLLSDPSSTLDASRLISEACKKHGFFLVVNHGISEELISDAHEYTS 113

Query: 63  EIFNLPSETKLKI-----GPLSSVNTYTPHFIAS-PFFETLRVSGPNFLASAQNSAEFLF 122
             F++P   K ++       +   +++T  F    P+ ETL     + ++ +++  ++  
Sbjct: 114 RFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCDDMSRSKSVQDYFC 173

Query: 123 NQ--KSSHQFSEILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINN 182
           +        F ++ QEY + M+ LS KI++  L+    G ++ Y    F+     +R+N 
Sbjct: 174 DALGHGFQPFGKVYQEYCEAMSSLSLKIME--LLGLSLGVKRDYFREFFEENDSIMRLNY 233

Query: 183 YTPPETLEDDEEVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVN 242
           Y  P  ++ D  + G G H D + +TI++QD   GLQV   E +W  I P    +A VVN
Sbjct: 234 Y--PPCIKPDLTL-GTGPHCDPTSLTILHQDHVNGLQV-FVENQWRSIRP--NPKAFVVN 293

Query: 243 IGDLLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYK 302
           IGD   A SNDR +S  HR V+   +  R S+AFF   + ++VV+ P +L++    R Y 
Sbjct: 294 IGDTFMALSNDRYKSCLHRAVVNSES-ERKSLAFFLCPKKDRVVTPPRELLDSITSRRYP 353

Query: 303 PFVCSDYLKFRENDER 306
            F  S +L+F +   R
Sbjct: 354 DFTWSMFLEFTQKHYR 360

[Close]
BLAST of CsaV3_6G000540 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 137.1 bits (344), Expect = 2.5e-32
Identity = 101/301 (33.55%), Postives = 148/301 (49.17%), Query Frame = 0 

Query: 10  LPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPSETKLK 69
           +P++D+S   +P   + + DA K WG F I NHGIS+++ + + S S  +F++PSE KL+
Sbjct: 49  IPVIDLS---NPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLFDMPSERKLE 108

Query: 70  IGPL-SSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSH---QFSEILQE 129
                  V+ Y    I SPFFE    S       A +S    FN    H   ++  I+QE
Sbjct: 109 AASSDKGVSGYGEPRI-SPFFEKKMWS--EGFTIADDSYRNHFNTLWPHDHTKYCGIIQE 168

Query: 130 YGDKMTELSRKIIKNILMSFGEGFE-----KKYHEPHFKNCHGYLRINNYTPPETLEDDE 189
           Y D+M +L+ +++  IL S G   E      K  +   K   G +R+N+Y       + E
Sbjct: 169 YVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHY---PVCPEPE 228

Query: 190 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 249
              GL  HTD + +TI++Q  TGGLQV   E  WV + P      LVVNIGDL    SN 
Sbjct: 229 RAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPA--PGVLVVNIGDLFHILSNG 288

Query: 250 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP-EDLVEEGGERMYKPFVCSDYLKF 301
           ++ S  HR  +     +R SIA+ WG     V  AP   L       +Y+     +YL+ 
Sbjct: 289 KIPSVVHRAKVNHTR-SRISIAYLWGGPAGDVQIAPISKLTGPAEPSLYRSITWKEYLQI 337

The following BLAST results are available for this feature:
BLAST of CsaV3_6G000540 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004138503.24.8e-187100.00gibberellin 20-oxidase-like protein [Cucumis sativus] >KGN45657.1 hypothetical p... [more]
XP_008458231.12.6e-17795.02PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo] >TY... [more]
XP_038875412.14.5e-16989.22gibberellin 20-oxidase-like protein [Benincasa hispida][more]
KAG6575175.11.8e-16588.41Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. soro... [more]
KAG7013737.11.8e-16588.41Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. argy... [more]
BLAST of CsaV3_6G000540 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4KBY02.7e-10056.10Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310... [more]
Q391101.6e-3130.38Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2[more]
Q9C9713.5e-3133.55Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ... [more]
Q9LHN84.6e-3130.52Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 S... [more]
Q9C8991.0e-3031.77Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 S... [more]
BLAST of CsaV3_6G000540 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KB922.3e-187100.00Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G... [more]
A0A5D3BVZ11.3e-17795.02Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa O... [more]
A0A1S3C8M61.3e-17795.02probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1H6328.6e-16688.41gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC11146035... [more]
A0A6J1KY831.1e-16589.13gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111499287 ... [more]
BLAST of CsaV3_6G000540 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G23340.14.3e-10959.252-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G51310.11.9e-10156.102-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G23340.24.2e-7263.862-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G25420.11.1e-3230.382-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G80330.12.5e-3233.55gibberellin 3-oxidase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 2..299
e-value: 7.7E-70
score: 238.0
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 10..75
e-value: 2.6E-15
score: 57.2
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 166..264
e-value: 6.9E-21
score: 74.6
NoneNo IPR availablePANTHERPTHR47990:SF52GIBBERELLIN 3-BETA-DIOXYGENASE 4coord: 4..318
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 4..318
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 7..303
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 161..267
score: 11.546795

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_6G000540.1CsaV3_6G000540.1mRNA