CsaV3_6G000270 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_6G000270
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Descriptionprotein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic
Locationchr6: 192137 .. 195201 (+)
RNA-Seq ExpressionCsaV3_6G000270
SyntenyCsaV3_6G000270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAAGCTAAGATGGGCAATGGACGGGCAAGGCTTCTGGGATCTCGATGTTTCAACGTCTAGAACACTCGATGGCTCTGCTTCCCCGGTTCCTTCTCCTTTACACTTACTTCCTTTGGGATTATCCAGAGGCGTTCGTCTTTCCAGAGCCAAGCAGATCGATTTTATGCAGCGTTTCATGGCTGCTCCTTTTGTTCCTTCTTATTCCCCCTCCCATGGCTTCTCTCTTCAACGCGTCTTCTCCGTACCCTTTTCAGATTCTGGGTAACTCAATCCTCTCTTTTTTGTTATTGGGTTCTTCTCATTCTTATGCTGGCATTTACATTTGTATTGATTCTCCATTAAATCTGCCTTAAAAGCACTGCAGCTCCATTACTCTCTTAGGCCAGTTCAATTTGCAGAAATTCATGTCCTCTCTGATGAAAACTGGTTCTGGAGAGATGTGTCAGTCGTATTCCTCACTTCTGCAATATATTGGAAGGCATCTTTACCAACGGTCTTTGTATGCTGTTGGTATCTCTGCCGATATCTTGTTACCACCCGATGATTCGCTGATGATTAGCTTCGATGGATATGGTGACAGTGATATAGTTCGAACAAAAGCAGTATTTCACCGCAAGGCATGTATATTTCTGGCACTTAGTAGAATTGTAAATTATGTATGCTGGTTGTTCTTGTTCTTGTTTTGATGTAGATTGTTTGTTTACGTCGTTGCAGTTCCTACATCATGATCTAACAGTGGAGGCACTTTCTCCAGGACTTTTTATGGAGAAATGTGGTAGATATTGGGATGTGCCTTCTTCATTGGTTGTTGATCTAGGTTCTGTTGCTTCCGATTCGGGTTTGAGTTACCATTTGTCTATGCACCAGAATGCTGGGTTTCCCTCACAATTGGGAAGTGAACCGACCCATTCTGCTCCTTTCTGTTTACTTCCTGGTCTATCAGCCAAGGCTGCGTTTGCCTTTAAGAAGAACTTTGAAATTTGGAGAAGTAATGCCAAGAAGTTAAAGATGGTGCAACCTTATGACATTTTTCTATCAACTCCTCATGTTTCATTGTCAGCGATCATTGGTATGAATCCACACTGATTGTCCTTCAATGAAACTTCTGATTCTATGTTTAGCCCCCTAAAGCTATTGCCATTTTTCAATTCTGTCTCTAAAAATCTCACTAGCCCTCCAAGATTAAGGATTCCAACCTTAACTTAACCTCTTTGATTCTTTTGTTTGTTTGTTTTAATCTTACCTCAGAAGTTTAGGTGTTGATGTTCATAGAAAATAATGTAAATGTTATGAGATCATACATTTTTGGTCAGTTTCAGTCACGTTAATGGGTAACAGCCTTACTTGAACTCTATCAGTTCTTTAGGTTGTAGAATTGTGTCCTTGAGTTTTAAAGATCACATTAATGAGTAGGAACTTCTTGAAGTTTTTTGACGTAGTGAGGCTATATGGATGATGGTACGCATAAAATGGGGGAAGGTTTAAGGACCATATTATAATTGTGACCTTATTTTTATTCGCATTTGCAGTTGCAATTGCTATCTTTGGCCATCAAATGCAGGATTTGACAAACTCAGAAATGATAATAGACACAGTTCATCCTTAACCCTTCTGTGTTCAAAATTCTCTTTTGCTTTACGTATAAAATTTTCGAACTATTGTTTTGCATCAGGAAAAGAAGAAATTTAGTTTAACTCTTTAAGATATTTTAGTTATTATATAGTTTTGCAGATGGTATGAAGCTGCAAGCCGTTCATTTGCAAATTATACTTTACAATTTCCACTAATTTTGGCATCTCTATTGATCTTAAAAGGTGCTGTAGCTACTTCCTACTTTGGAGACGATTTGGCTAGATCAGCAGCACAAGACAGTCTTGAGAAATTCAAAGGATTTTACATGAAGAGTTCTAGAATTAGATCGACTGTTTTCGCGGATTTATTCACTTCTATTTCTTTTTCAGCTCAGTATGGGATGTTTCAGAAGAAATATCTGGATCTTACCCGATTTTCTGCTTGTATGGATTTCCATTCTGGCTCCAAGTTTCTTTCAGGATCCATGCTTTTGATAGATGATCTTTCCAACTCCCGGCACCCAAAAACTGAATCTGTGAAAGCGACCTTGCCTAATGCAAGATTTTCCATTCAGCAACAGGTATGTCCGTCTTCCATTCAGCTGCTGAATGTGGACTAAATCAATAATGATAAACTGTAAAGAAAAACCAGTATTTGTATCCCAATCCTGTTCTTTTTTAATCTAGAAAGTTCCGGTTTATATTAGTTTTGAAAAATTCTTATTGGTTGTCTAGGGCACTAAGTGAGTTATGAAGTCTGGAGTTAATGTATTGAAATTTGGAAGTCTATATTTGAGGTGCAAAGTTGTAGGATGAAATTCATGGACAAATTTGCAAATTTCAATAGTAAAAACATCATTGAAATTGAGTTGTTTAACACCACTCATGAAGTTGGCTCATGAGTTAGTTGGGTGGGCTACTTCTAATAAGGAGGGGTGTGTGACGTGAAGGTTTTGACTTTTGTCTTTCTTAGCTTGGTTTGGTTATTGACCATGATGCTTATATTTGTATCTGTGAGGGGACGATTCTGCTTAATGAAGATTTATGATATCTTTTTTCCAAAAAAAAAAAATCTTAAGAAGTTGGTGGGCCTAGTAGACCTCTTAGTGAAAGTGTCTCACGAGTTTGAACACTTGTAGAAATTTCCATGGTTTCAGATAAGTTTGAAAGGAAAATGATAATGTATATCATGTTTGGAATTGATTGGCAACAACTCATTGGAAAACCTTCGCAAATCAGGAATTATGATATGAGTTTTGTGATGGATTTCTTGAAACATTGGTTGCAGATTGCTGGACCTGTGAGCTTTAGAGCAGATACAGGAGTTGCAATAGATTTGAATAAAGCAGGGTGGGATTTATTACGAGTGGAAGAGCCTACATTTGCCTTGGAATATGCGTTGCATGTCCTTGGTTCAGCTAAGGCAATTGCTTGGTATTCACCAAAGCATAGAGAATTTATGGTAGAGCTTCGTTTCTACGAGACCTGA

mRNA sequence

ATGAAGAAGCTAAGATGGGCAATGGACGGGCAAGGCTTCTGGGATCTCGATGTTTCAACGTCTAGAACACTCGATGGCTCTGCTTCCCCGGTTCCTTCTCCTTTACACTTACTTCCTTTGGGATTATCCAGAGGCGTTCGTCTTTCCAGAGCCAAGCAGATCGATTTTATGCAGCGTTTCATGGCTGCTCCTTTTGTTCCTTCTTATTCCCCCTCCCATGGCTTCTCTCTTCAACGCGTCTTCTCCGTACCCTTTTCAGATTCTGGCTCCATTACTCTCTTAGGCCAGTTCAATTTGCAGAAATTCATGTCCTCTCTGATGAAAACTGGTTCTGGAGAGATGTGTCAGTCGTATTCCTCACTTCTGCAATATATTGGAAGGCATCTTTACCAACGGTCTTTGTATGCTGTTGGTATCTCTGCCGATATCTTGTTACCACCCGATGATTCGCTGATGATTAGCTTCGATGGATATGGTGACAGTGATATAGTTCGAACAAAAGCAGTATTTCACCGCAAGTTCCTACATCATGATCTAACAGTGGAGGCACTTTCTCCAGGACTTTTTATGGAGAAATGTGGTAGATATTGGGATGTGCCTTCTTCATTGGTTGTTGATCTAGGTTCTGTTGCTTCCGATTCGGGTTTGAGTTACCATTTGTCTATGCACCAGAATGCTGGGTTTCCCTCACAATTGGGAAGTGAACCGACCCATTCTGCTCCTTTCTGTTTACTTCCTGGTCTATCAGCCAAGGCTGCGTTTGCCTTTAAGAAGAACTTTGAAATTTGGAGAAGTAATGCCAAGAAGTTAAAGATGGTGCAACCTTATGACATTTTTCTATCAACTCCTCATGTTTCATTGTCAGCGATCATTGGTGCTGTAGCTACTTCCTACTTTGGAGACGATTTGGCTAGATCAGCAGCACAAGACAGTCTTGAGAAATTCAAAGGATTTTACATGAAGAGTTCTAGAATTAGATCGACTGTTTTCGCGGATTTATTCACTTCTATTTCTTTTTCAGCTCAGTATGGGATGTTTCAGAAGAAATATCTGGATCTTACCCGATTTTCTGCTTGTATGGATTTCCATTCTGGCTCCAAGTTTCTTTCAGGATCCATGCTTTTGATAGATGATCTTTCCAACTCCCGGCACCCAAAAACTGAATCTGTGAAAGCGACCTTGCCTAATGCAAGATTTTCCATTCAGCAACAGATTGCTGGACCTGTGAGCTTTAGAGCAGATACAGGAGTTGCAATAGATTTGAATAAAGCAGGGTGGGATTTATTACGAGTGGAAGAGCCTACATTTGCCTTGGAATATGCGTTGCATGTCCTTGGTTCAGCTAAGGCAATTGCTTGGTATTCACCAAAGCATAGAGAATTTATGGTAGAGCTTCGTTTCTACGAGACCTGA

Coding sequence (CDS)

ATGAAGAAGCTAAGATGGGCAATGGACGGGCAAGGCTTCTGGGATCTCGATGTTTCAACGTCTAGAACACTCGATGGCTCTGCTTCCCCGGTTCCTTCTCCTTTACACTTACTTCCTTTGGGATTATCCAGAGGCGTTCGTCTTTCCAGAGCCAAGCAGATCGATTTTATGCAGCGTTTCATGGCTGCTCCTTTTGTTCCTTCTTATTCCCCCTCCCATGGCTTCTCTCTTCAACGCGTCTTCTCCGTACCCTTTTCAGATTCTGGCTCCATTACTCTCTTAGGCCAGTTCAATTTGCAGAAATTCATGTCCTCTCTGATGAAAACTGGTTCTGGAGAGATGTGTCAGTCGTATTCCTCACTTCTGCAATATATTGGAAGGCATCTTTACCAACGGTCTTTGTATGCTGTTGGTATCTCTGCCGATATCTTGTTACCACCCGATGATTCGCTGATGATTAGCTTCGATGGATATGGTGACAGTGATATAGTTCGAACAAAAGCAGTATTTCACCGCAAGTTCCTACATCATGATCTAACAGTGGAGGCACTTTCTCCAGGACTTTTTATGGAGAAATGTGGTAGATATTGGGATGTGCCTTCTTCATTGGTTGTTGATCTAGGTTCTGTTGCTTCCGATTCGGGTTTGAGTTACCATTTGTCTATGCACCAGAATGCTGGGTTTCCCTCACAATTGGGAAGTGAACCGACCCATTCTGCTCCTTTCTGTTTACTTCCTGGTCTATCAGCCAAGGCTGCGTTTGCCTTTAAGAAGAACTTTGAAATTTGGAGAAGTAATGCCAAGAAGTTAAAGATGGTGCAACCTTATGACATTTTTCTATCAACTCCTCATGTTTCATTGTCAGCGATCATTGGTGCTGTAGCTACTTCCTACTTTGGAGACGATTTGGCTAGATCAGCAGCACAAGACAGTCTTGAGAAATTCAAAGGATTTTACATGAAGAGTTCTAGAATTAGATCGACTGTTTTCGCGGATTTATTCACTTCTATTTCTTTTTCAGCTCAGTATGGGATGTTTCAGAAGAAATATCTGGATCTTACCCGATTTTCTGCTTGTATGGATTTCCATTCTGGCTCCAAGTTTCTTTCAGGATCCATGCTTTTGATAGATGATCTTTCCAACTCCCGGCACCCAAAAACTGAATCTGTGAAAGCGACCTTGCCTAATGCAAGATTTTCCATTCAGCAACAGATTGCTGGACCTGTGAGCTTTAGAGCAGATACAGGAGTTGCAATAGATTTGAATAAAGCAGGGTGGGATTTATTACGAGTGGAAGAGCCTACATTTGCCTTGGAATATGCGTTGCATGTCCTTGGTTCAGCTAAGGCAATTGCTTGGTATTCACCAAAGCATAGAGAATTTATGGTAGAGCTTCGTTTCTACGAGACCTGA

Protein sequence

MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRFMAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSSLLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLTVEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSAPFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFGDDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACMDFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAIDLNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYET*
Homology
BLAST of CsaV3_6G000270 vs. NCBI nr
Match: XP_004138517.2 (protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic isoform X1 [Cucumis sativus] >KGN45628.1 hypothetical protein Csa_005594 [Cucumis sativus])

HSP 1 Score: 940.6 bits (2430), Expect = 5.0e-270
Identity = 470/470 (100.00%), Postives = 470/470 (100.00%), Query Frame = 0

Query: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60
           MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF
Sbjct: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60

Query: 61  MAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSS 120
           MAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSS
Sbjct: 61  MAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSS 120

Query: 121 LLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 180
           LLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT
Sbjct: 121 LLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 180

Query: 181 VEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSA 240
           VEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSA
Sbjct: 181 VEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSA 240

Query: 241 PFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 300
           PFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG
Sbjct: 241 PFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 300

Query: 301 DDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACM 360
           DDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACM
Sbjct: 301 DDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACM 360

Query: 361 DFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAID 420
           DFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAID
Sbjct: 361 DFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAID 420

Query: 421 LNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYET 471
           LNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYET
Sbjct: 421 LNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYET 470

BLAST of CsaV3_6G000270 vs. NCBI nr
Match: TYK02989.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 4 [Cucumis melo var. makuwa])

HSP 1 Score: 882.5 bits (2279), Expect = 1.6e-252
Identity = 441/470 (93.83%), Postives = 453/470 (96.38%), Query Frame = 0

Query: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60
           MKKLRWAMD  GFWDLDVSTSRTLDGSASPVPSP HLLPLGLSRGVRLSRAKQIDFMQ F
Sbjct: 332 MKKLRWAMD--GFWDLDVSTSRTLDGSASPVPSPFHLLPLGLSRGVRLSRAKQIDFMQSF 391

Query: 61  MAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSS 120
           M APFVPSYSPSHGFSLQRVFS+PFSDSGSITLLGQFNLQKFMSSLMKTGSGEM QS+SS
Sbjct: 392 MVAPFVPSYSPSHGFSLQRVFSIPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMGQSFSS 451

Query: 121 LLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 180
            +Q IGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT
Sbjct: 452 FIQCIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 511

Query: 181 VEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSA 240
           +EALSPGLFM+K GRYWDVPSSLVVDLGS ASDSGLSYHLSMHQN GFPS LGSEPTHSA
Sbjct: 512 MEALSPGLFMDKSGRYWDVPSSLVVDLGSAASDSGLSYHLSMHQNTGFPSPLGSEPTHSA 571

Query: 241 PFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 300
           PFCL PGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG
Sbjct: 572 PFCLFPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 631

Query: 301 DDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACM 360
           DDL RSAAQ SL +FKGFYM++SRIRST+FADLFTSISFSAQYGMFQKKYLDLTRFSACM
Sbjct: 632 DDLVRSAAQGSLAEFKGFYMQTSRIRSTIFADLFTSISFSAQYGMFQKKYLDLTRFSACM 691

Query: 361 DFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAID 420
           DFHSGSKFLSGSMLLIDDLSNSRHPKTE+VKATLPNARFSIQQQIAGPVSFRAD+GVAID
Sbjct: 692 DFHSGSKFLSGSMLLIDDLSNSRHPKTEAVKATLPNARFSIQQQIAGPVSFRADSGVAID 751

Query: 421 LNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYET 471
           LNKAGWDLLRV+EPTFALEYAL VLGSAKAIAWYSPKHREFMVELRFYET
Sbjct: 752 LNKAGWDLLRVDEPTFALEYALQVLGSAKAIAWYSPKHREFMVELRFYET 799

BLAST of CsaV3_6G000270 vs. NCBI nr
Match: XP_008458282.1 (PREDICTED: protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucumis melo])

HSP 1 Score: 882.5 bits (2279), Expect = 1.6e-252
Identity = 441/470 (93.83%), Postives = 453/470 (96.38%), Query Frame = 0

Query: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60
           MKKLRWAMD  GFWDLDVSTSRTLDGSASPVPSP HLLPLGLSRGVRLSRAKQIDFMQ F
Sbjct: 1   MKKLRWAMD--GFWDLDVSTSRTLDGSASPVPSPFHLLPLGLSRGVRLSRAKQIDFMQSF 60

Query: 61  MAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSS 120
           M APFVPSYSPSHGFSLQRVFS+PFSDSGSITLLGQFNLQKFMSSLMKTGSGEM QS+SS
Sbjct: 61  MVAPFVPSYSPSHGFSLQRVFSIPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMGQSFSS 120

Query: 121 LLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 180
            +Q IGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT
Sbjct: 121 FIQCIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 180

Query: 181 VEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSA 240
           +EALSPGLFM+K GRYWDVPSSLVVDLGS ASDSGLSYHLSMHQN GFPS LGSEPTHSA
Sbjct: 181 MEALSPGLFMDKSGRYWDVPSSLVVDLGSAASDSGLSYHLSMHQNTGFPSPLGSEPTHSA 240

Query: 241 PFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 300
           PFCL PGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG
Sbjct: 241 PFCLFPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 300

Query: 301 DDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACM 360
           DDL RSAAQ SL +FKGFYM++SRIRST+FADLFTSISFSAQYGMFQKKYLDLTRFSACM
Sbjct: 301 DDLVRSAAQGSLAEFKGFYMQTSRIRSTIFADLFTSISFSAQYGMFQKKYLDLTRFSACM 360

Query: 361 DFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAID 420
           DFHSGSKFLSGSMLLIDDLSNSRHPKTE+VKATLPNARFSIQQQIAGPVSFRAD+GVAID
Sbjct: 361 DFHSGSKFLSGSMLLIDDLSNSRHPKTEAVKATLPNARFSIQQQIAGPVSFRADSGVAID 420

Query: 421 LNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYET 471
           LNKAGWDLLRV+EPTFALEYAL VLGSAKAIAWYSPKHREFMVELRFYET
Sbjct: 421 LNKAGWDLLRVDEPTFALEYALQVLGSAKAIAWYSPKHREFMVELRFYET 468

BLAST of CsaV3_6G000270 vs. NCBI nr
Match: XP_038875869.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Benincasa hispida] >XP_038875870.1 protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Benincasa hispida])

HSP 1 Score: 814.7 bits (2103), Expect = 4.2e-232
Identity = 405/470 (86.17%), Postives = 436/470 (92.77%), Query Frame = 0

Query: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60
           MKKLRWAMDGQGFWDLDVST RTLDGSASPVPS LHLLPLGLSRGVRLSRAKQIDFMQ F
Sbjct: 2   MKKLRWAMDGQGFWDLDVSTPRTLDGSASPVPSHLHLLPLGLSRGVRLSRAKQIDFMQSF 61

Query: 61  MAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSS 120
           MAAPFVPSYSPSHGFSLQRVFS+PFSDSGS+TLLGQFNLQKF+SSL K+G G+M QS SS
Sbjct: 62  MAAPFVPSYSPSHGFSLQRVFSIPFSDSGSVTLLGQFNLQKFISSLKKSGVGDMGQSLSS 121

Query: 121 LLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 180
            LQ IGRHL  RSLYA+GIS+DILLPPDDSLMISFDGYGD++IVRTKAVFH KFLHHDLT
Sbjct: 122 FLQCIGRHLCHRSLYALGISSDILLPPDDSLMISFDGYGDNEIVRTKAVFHHKFLHHDLT 181

Query: 181 VEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSA 240
           +EA SPGLF++K G+YWDVPS+LVVDLGS AS+SGLSYHLSMHQN G PSQ GSE   S+
Sbjct: 182 MEAFSPGLFVDKSGKYWDVPSALVVDLGSAASESGLSYHLSMHQNTGSPSQSGSEQARSS 241

Query: 241 PFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 300
           P CLLPGLSAKAAFAFKKN EIWRSNAKKLKMVQPYDIFLSTPHVSLS IIGAVAT+YFG
Sbjct: 242 PLCLLPGLSAKAAFAFKKNLEIWRSNAKKLKMVQPYDIFLSTPHVSLSGIIGAVATTYFG 301

Query: 301 DDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACM 360
           D+  RSAAQDSL +FKG Y+++SRIRSTVFAD+F SISFSAQYGMFQ+KYLDLT FSA M
Sbjct: 302 DNSIRSAAQDSLPEFKGLYLQTSRIRSTVFADVFASISFSAQYGMFQRKYLDLTCFSARM 361

Query: 361 DFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAID 420
           DFHSGSKFLSG+MLLIDDLSNSRHP+TESV+ATLP+ARFS+QQQIAGPVSFRAD+GVAID
Sbjct: 362 DFHSGSKFLSGAMLLIDDLSNSRHPRTESVRATLPSARFSLQQQIAGPVSFRADSGVAID 421

Query: 421 LNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYET 471
           LNKAGW LL V+EPTFALEYAL VLGSAKAIAWYSPKHREFMVELRFYET
Sbjct: 422 LNKAGWGLLGVDEPTFALEYALQVLGSAKAIAWYSPKHREFMVELRFYET 471

BLAST of CsaV3_6G000270 vs. NCBI nr
Match: XP_023548884.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023548885.1 protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023548886.1 protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023548887.1 protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 786.2 bits (2029), Expect = 1.6e-223
Identity = 393/469 (83.80%), Postives = 425/469 (90.62%), Query Frame = 0

Query: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60
           MKKLRW M+GQ FWDLDVST RTLDGSASPVP+ L LLPLGLSRGVRLSRAKQIDFMQ+F
Sbjct: 2   MKKLRWTMEGQSFWDLDVSTPRTLDGSASPVPTDLQLLPLGLSRGVRLSRAKQIDFMQQF 61

Query: 61  MAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSS 120
           MAAPFVPSY+PSHGFSLQRVFS+PFSDSGS TLLGQFN+QKF+SSL K+G GEM QS SS
Sbjct: 62  MAAPFVPSYTPSHGFSLQRVFSIPFSDSGSATLLGQFNVQKFVSSLKKSGFGEMGQSISS 121

Query: 121 LLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 180
           LLQ IGRHL  RSLYA GIS+DILL PDD+L+ISFDGYGDSDI+RTKAV H KFLHHDLT
Sbjct: 122 LLQGIGRHLRDRSLYAFGISSDILLTPDDALLISFDGYGDSDILRTKAVLHHKFLHHDLT 181

Query: 181 VEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSA 240
           +EALSPGLF++K G+YWDVPSSLV+DLGS A+DSGLSYHLSMH NAG PSQ GSE T  A
Sbjct: 182 MEALSPGLFVDKSGKYWDVPSSLVIDLGSAATDSGLSYHLSMHHNAGSPSQSGSEQTCMA 241

Query: 241 PFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 300
           PFCLLPGLSAKAAFA KKN EIWRSNAKKLK VQPYDIFLS PHVSLS IIGAVATSYFG
Sbjct: 242 PFCLLPGLSAKAAFALKKNLEIWRSNAKKLKRVQPYDIFLSNPHVSLSGIIGAVATSYFG 301

Query: 301 DDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACM 360
           D  A SAA+ SL++FKG YM++SRIRSTVFAD+F SISFSAQYGMFQ+ +LDLTRFS   
Sbjct: 302 DISAGSAAEGSLQEFKGLYMQTSRIRSTVFADVFASISFSAQYGMFQRNFLDLTRFSGRF 361

Query: 361 DFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAID 420
           DFHSGSKFLSG+MLLI+DLSNS+HP+TESVKATLPNARFS QQQIAGPVSFRADTGVAID
Sbjct: 362 DFHSGSKFLSGAMLLIEDLSNSQHPRTESVKATLPNARFSFQQQIAGPVSFRADTGVAID 421

Query: 421 LNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYE 470
           L+KAGW  L+VEEPTFALEYALHVLGSAKAIAWYSPK REFMVELRFYE
Sbjct: 422 LSKAGWGSLQVEEPTFALEYALHVLGSAKAIAWYSPKQREFMVELRFYE 470

BLAST of CsaV3_6G000270 vs. ExPASy Swiss-Prot
Match: Q9M903 (Protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TGD4 PE=1 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 2.9e-119
Identity = 229/484 (47.31%), Postives = 321/484 (66.32%), Query Frame = 0

Query: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60
           M ++RW  +G   WDLD+ST  TL+G+A  VP     LPLGLSRG RLSR KQ++F  RF
Sbjct: 1   MNRMRWVGEGD-IWDLDMSTPVTLEGTARAVPD--DPLPLGLSRGTRLSRPKQVEFFHRF 60

Query: 61  MAAPFVPSYSP---------SHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKT-- 120
           MA+P +PS+SP           GFSLQRV ++PFS++  ++LLGQF++Q+F++ + KT  
Sbjct: 61  MASPLIPSFSPIRPNTGDGGGGGFSLQRVLTLPFSNNWLVSLLGQFDVQRFVTEIDKTKA 120

Query: 121 -GSGEMCQSYSSLLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGY-GDSD-IVRT 180
            G G    + +S L  IG+HL  +SLYA+G  ++ LL PDD+L++S+D Y GD D   R 
Sbjct: 121 FGRGS-SSTVASRLNTIGKHLKDKSLYALGFCSEFLLSPDDTLLLSYDAYKGDLDKNPRA 180

Query: 181 KAVFHRKFLHHDLTVEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNA 240
           KA+F+ +F  H+LT EA+ PGLF++K G YWDVP S+ +DL S+ ++SG SYHL +H N+
Sbjct: 181 KAIFNHEFPLHNLTAEAVWPGLFVDKHGEYWDVPLSMAIDLASLPAESGPSYHLCLHHNS 240

Query: 241 GFPSQLGSEPTHSAPFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVS 300
           G P +L S+     P  LLPGLS K+A +++ N ++WR    KL+  +PYD+FLS+PHV+
Sbjct: 241 GSPKKLHSDTMEVPPPSLLPGLSLKSAVSYRTNMDLWRGTTPKLETCKPYDVFLSSPHVA 300

Query: 301 LSAIIGAVATSYFGDDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMF 360
           +S IIG+V T+ FG++  RS  ++  E   GF +    + S   AD     S +AQYG F
Sbjct: 301 VSGIIGSVMTAAFGENSIRSKFENDSEGVGGFSLHFPSVNSGFMADALGRASLTAQYGNF 360

Query: 361 QKKYLDLTRFSACMDFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIA 420
           QK + DLTRF A +DF  G +FL+G+  +  DL NSR P  E+ +   P    S+QQQI 
Sbjct: 361 QKFFFDLTRFHARLDFPHGLRFLTGATSVAQDLLNSRQPSLEAFQKICPEVLVSLQQQIV 420

Query: 421 GPVSFRADTGVAIDLNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELR 471
           GP SF+ ++G+ IDL + G + + V++  FA+EYAL VL SAKA+  YSPK  EFMVELR
Sbjct: 421 GPFSFKVESGIEIDL-RNGANPVTVDKTVFAIEYALQVLLSAKAVVSYSPKQNEFMVELR 479

BLAST of CsaV3_6G000270 vs. ExPASy TrEMBL
Match: A0A0A0K824 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G001740 PE=4 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 2.4e-270
Identity = 470/470 (100.00%), Postives = 470/470 (100.00%), Query Frame = 0

Query: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60
           MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF
Sbjct: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60

Query: 61  MAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSS 120
           MAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSS
Sbjct: 61  MAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSS 120

Query: 121 LLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 180
           LLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT
Sbjct: 121 LLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 180

Query: 181 VEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSA 240
           VEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSA
Sbjct: 181 VEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSA 240

Query: 241 PFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 300
           PFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG
Sbjct: 241 PFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 300

Query: 301 DDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACM 360
           DDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACM
Sbjct: 301 DDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACM 360

Query: 361 DFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAID 420
           DFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAID
Sbjct: 361 DFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAID 420

Query: 421 LNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYET 471
           LNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYET
Sbjct: 421 LNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYET 470

BLAST of CsaV3_6G000270 vs. ExPASy TrEMBL
Match: A0A5D3BY40 (Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00780 PE=3 SV=1)

HSP 1 Score: 882.5 bits (2279), Expect = 7.9e-253
Identity = 441/470 (93.83%), Postives = 453/470 (96.38%), Query Frame = 0

Query: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60
           MKKLRWAMD  GFWDLDVSTSRTLDGSASPVPSP HLLPLGLSRGVRLSRAKQIDFMQ F
Sbjct: 332 MKKLRWAMD--GFWDLDVSTSRTLDGSASPVPSPFHLLPLGLSRGVRLSRAKQIDFMQSF 391

Query: 61  MAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSS 120
           M APFVPSYSPSHGFSLQRVFS+PFSDSGSITLLGQFNLQKFMSSLMKTGSGEM QS+SS
Sbjct: 392 MVAPFVPSYSPSHGFSLQRVFSIPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMGQSFSS 451

Query: 121 LLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 180
            +Q IGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT
Sbjct: 452 FIQCIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 511

Query: 181 VEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSA 240
           +EALSPGLFM+K GRYWDVPSSLVVDLGS ASDSGLSYHLSMHQN GFPS LGSEPTHSA
Sbjct: 512 MEALSPGLFMDKSGRYWDVPSSLVVDLGSAASDSGLSYHLSMHQNTGFPSPLGSEPTHSA 571

Query: 241 PFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 300
           PFCL PGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG
Sbjct: 572 PFCLFPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 631

Query: 301 DDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACM 360
           DDL RSAAQ SL +FKGFYM++SRIRST+FADLFTSISFSAQYGMFQKKYLDLTRFSACM
Sbjct: 632 DDLVRSAAQGSLAEFKGFYMQTSRIRSTIFADLFTSISFSAQYGMFQKKYLDLTRFSACM 691

Query: 361 DFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAID 420
           DFHSGSKFLSGSMLLIDDLSNSRHPKTE+VKATLPNARFSIQQQIAGPVSFRAD+GVAID
Sbjct: 692 DFHSGSKFLSGSMLLIDDLSNSRHPKTEAVKATLPNARFSIQQQIAGPVSFRADSGVAID 751

Query: 421 LNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYET 471
           LNKAGWDLLRV+EPTFALEYAL VLGSAKAIAWYSPKHREFMVELRFYET
Sbjct: 752 LNKAGWDLLRVDEPTFALEYALQVLGSAKAIAWYSPKHREFMVELRFYET 799

BLAST of CsaV3_6G000270 vs. ExPASy TrEMBL
Match: A0A1S3C837 (protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103497747 PE=4 SV=1)

HSP 1 Score: 882.5 bits (2279), Expect = 7.9e-253
Identity = 441/470 (93.83%), Postives = 453/470 (96.38%), Query Frame = 0

Query: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60
           MKKLRWAMD  GFWDLDVSTSRTLDGSASPVPSP HLLPLGLSRGVRLSRAKQIDFMQ F
Sbjct: 1   MKKLRWAMD--GFWDLDVSTSRTLDGSASPVPSPFHLLPLGLSRGVRLSRAKQIDFMQSF 60

Query: 61  MAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSS 120
           M APFVPSYSPSHGFSLQRVFS+PFSDSGSITLLGQFNLQKFMSSLMKTGSGEM QS+SS
Sbjct: 61  MVAPFVPSYSPSHGFSLQRVFSIPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMGQSFSS 120

Query: 121 LLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 180
            +Q IGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT
Sbjct: 121 FIQCIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 180

Query: 181 VEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSA 240
           +EALSPGLFM+K GRYWDVPSSLVVDLGS ASDSGLSYHLSMHQN GFPS LGSEPTHSA
Sbjct: 181 MEALSPGLFMDKSGRYWDVPSSLVVDLGSAASDSGLSYHLSMHQNTGFPSPLGSEPTHSA 240

Query: 241 PFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 300
           PFCL PGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG
Sbjct: 241 PFCLFPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 300

Query: 301 DDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACM 360
           DDL RSAAQ SL +FKGFYM++SRIRST+FADLFTSISFSAQYGMFQKKYLDLTRFSACM
Sbjct: 301 DDLVRSAAQGSLAEFKGFYMQTSRIRSTIFADLFTSISFSAQYGMFQKKYLDLTRFSACM 360

Query: 361 DFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAID 420
           DFHSGSKFLSGSMLLIDDLSNSRHPKTE+VKATLPNARFSIQQQIAGPVSFRAD+GVAID
Sbjct: 361 DFHSGSKFLSGSMLLIDDLSNSRHPKTEAVKATLPNARFSIQQQIAGPVSFRADSGVAID 420

Query: 421 LNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYET 471
           LNKAGWDLLRV+EPTFALEYAL VLGSAKAIAWYSPKHREFMVELRFYET
Sbjct: 421 LNKAGWDLLRVDEPTFALEYALQVLGSAKAIAWYSPKHREFMVELRFYET 468

BLAST of CsaV3_6G000270 vs. ExPASy TrEMBL
Match: A0A6J1H3U0 (protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111460245 PE=4 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.3e-220
Identity = 386/469 (82.30%), Postives = 423/469 (90.19%), Query Frame = 0

Query: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60
           MKKLRW M+GQ FWDLDVST RTLDGSASPVP+ L LLPLGLSRGVRLSRAKQIDFMQ+F
Sbjct: 2   MKKLRWTMEGQSFWDLDVSTPRTLDGSASPVPTDLQLLPLGLSRGVRLSRAKQIDFMQQF 61

Query: 61  MAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSS 120
           MAAPFVPS++PSHGFSLQRVFS+PFSDSGS TLLGQFN+QKF+SSL K+G GEM QS SS
Sbjct: 62  MAAPFVPSFTPSHGFSLQRVFSIPFSDSGSATLLGQFNVQKFVSSLKKSGFGEMGQSISS 121

Query: 121 LLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 180
           LLQ IGRHL  RSLYA GIS+DILL PDD+L+ISFDGYGDSDI+RTKAV H KFLHHDLT
Sbjct: 122 LLQGIGRHLRDRSLYAFGISSDILLTPDDALLISFDGYGDSDILRTKAVLHHKFLHHDLT 181

Query: 181 VEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSA 240
           +EALSPGLF++K G+YWDVPSSLV+DLGS  +DSGLSYHLSMH NAG PSQ GSE T  A
Sbjct: 182 MEALSPGLFVDKSGKYWDVPSSLVIDLGSADTDSGLSYHLSMHHNAGSPSQSGSEQTCMA 241

Query: 241 PFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 300
           PFCLLPGLSAKAAFA KKN EIWRSNAKKLK VQPYDIFL+ PHVSLS IIGAVATSYFG
Sbjct: 242 PFCLLPGLSAKAAFALKKNLEIWRSNAKKLKRVQPYDIFLANPHVSLSGIIGAVATSYFG 301

Query: 301 DDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACM 360
           D  A SAA+ SL++F+G YM++SRIRSTVFAD+F SISFSAQYGMFQ+ +LDLTRFS   
Sbjct: 302 DISAGSAAEGSLQEFRGLYMQTSRIRSTVFADVFASISFSAQYGMFQRNFLDLTRFSGRF 361

Query: 361 DFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAID 420
           DFHSGSKFLSG+MLLI+DLSNS+HP+TESVKATLPNARFS QQQIAGPVSFRAD+GVAID
Sbjct: 362 DFHSGSKFLSGAMLLIEDLSNSQHPRTESVKATLPNARFSFQQQIAGPVSFRADSGVAID 421

Query: 421 LNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYE 470
           L+KAGW  L+VEEPTFALEYAL+ LGSAKAIAWYSPK REFMVELRFYE
Sbjct: 422 LSKAGWGSLQVEEPTFALEYALYALGSAKAIAWYSPKQREFMVELRFYE 470

BLAST of CsaV3_6G000270 vs. ExPASy TrEMBL
Match: A0A6J1KW75 (protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499258 PE=4 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.2e-219
Identity = 386/469 (82.30%), Postives = 420/469 (89.55%), Query Frame = 0

Query: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60
           MKKLRW M+GQ FWDLDVST RTLDGSASPVP+ L LLPLGLSRGVRLSRAKQIDFMQ+F
Sbjct: 2   MKKLRWTMEGQSFWDLDVSTPRTLDGSASPVPTDLQLLPLGLSRGVRLSRAKQIDFMQQF 61

Query: 61  MAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSS 120
           MAAPFVPSY+PSHGFSLQRVFS+PFSDSGS TLLGQFN+QKF+SSL K+G GEM QS SS
Sbjct: 62  MAAPFVPSYTPSHGFSLQRVFSIPFSDSGSATLLGQFNVQKFVSSLKKSGFGEMGQSLSS 121

Query: 121 LLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 180
           LLQ IGRHL  RSLYA GIS+DILL PDD+L+ISFDGYGDSD++RTKAV H KFLHHDLT
Sbjct: 122 LLQGIGRHLRDRSLYAFGISSDILLTPDDALLISFDGYGDSDVLRTKAVLHHKFLHHDLT 181

Query: 181 VEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSA 240
           +EALSPGLF++K G+YWDVPSSLV+DLGS A+DSGLSYHLSMH NAG PSQ GSE T  A
Sbjct: 182 MEALSPGLFVDKSGKYWDVPSSLVIDLGSAATDSGLSYHLSMHHNAGSPSQSGSEQTCMA 241

Query: 241 PFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 300
           PFCLLPGLSAKAAFA KKN EIWRSNAKKLK VQPYDIFLS PHVSLS IIGAVATSYF 
Sbjct: 242 PFCLLPGLSAKAAFALKKNLEIWRSNAKKLKRVQPYDIFLSNPHVSLSGIIGAVATSYFR 301

Query: 301 DDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACM 360
           D    SAA+ SL++FKG +M++SRIRSTVFAD+F SISFSAQYGMFQ  +LDLTRFS   
Sbjct: 302 DISVGSAAEGSLQEFKGLHMQTSRIRSTVFADVFASISFSAQYGMFQSNFLDLTRFSGRF 361

Query: 361 DFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAID 420
           DFHSGSKFLSG+MLLI+DLSNS+HP+TESVKATLPNARFS QQQIAGPVSFRADTGVAID
Sbjct: 362 DFHSGSKFLSGAMLLIEDLSNSQHPRTESVKATLPNARFSFQQQIAGPVSFRADTGVAID 421

Query: 421 LNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYE 470
           L+KAGW  L+VEEPTFALEYAL+ LGSAKAIAWYSPK  EFMVELRFYE
Sbjct: 422 LSKAGWGSLQVEEPTFALEYALYALGSAKAIAWYSPKQTEFMVELRFYE 470

BLAST of CsaV3_6G000270 vs. TAIR 10
Match: AT3G06960.1 (pigment defective 320 )

HSP 1 Score: 430.3 bits (1105), Expect = 2.1e-120
Identity = 229/484 (47.31%), Postives = 321/484 (66.32%), Query Frame = 0

Query: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60
           M ++RW  +G   WDLD+ST  TL+G+A  VP     LPLGLSRG RLSR KQ++F  RF
Sbjct: 1   MNRMRWVGEGD-IWDLDMSTPVTLEGTARAVPD--DPLPLGLSRGTRLSRPKQVEFFHRF 60

Query: 61  MAAPFVPSYSP---------SHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKT-- 120
           MA+P +PS+SP           GFSLQRV ++PFS++  ++LLGQF++Q+F++ + KT  
Sbjct: 61  MASPLIPSFSPIRPNTGDGGGGGFSLQRVLTLPFSNNWLVSLLGQFDVQRFVTEIDKTKA 120

Query: 121 -GSGEMCQSYSSLLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGY-GDSD-IVRT 180
            G G    + +S L  IG+HL  +SLYA+G  ++ LL PDD+L++S+D Y GD D   R 
Sbjct: 121 FGRGS-SSTVASRLNTIGKHLKDKSLYALGFCSEFLLSPDDTLLLSYDAYKGDLDKNPRA 180

Query: 181 KAVFHRKFLHHDLTVEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNA 240
           KA+F+ +F  H+LT EA+ PGLF++K G YWDVP S+ +DL S+ ++SG SYHL +H N+
Sbjct: 181 KAIFNHEFPLHNLTAEAVWPGLFVDKHGEYWDVPLSMAIDLASLPAESGPSYHLCLHHNS 240

Query: 241 GFPSQLGSEPTHSAPFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVS 300
           G P +L S+     P  LLPGLS K+A +++ N ++WR    KL+  +PYD+FLS+PHV+
Sbjct: 241 GSPKKLHSDTMEVPPPSLLPGLSLKSAVSYRTNMDLWRGTTPKLETCKPYDVFLSSPHVA 300

Query: 301 LSAIIGAVATSYFGDDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMF 360
           +S IIG+V T+ FG++  RS  ++  E   GF +    + S   AD     S +AQYG F
Sbjct: 301 VSGIIGSVMTAAFGENSIRSKFENDSEGVGGFSLHFPSVNSGFMADALGRASLTAQYGNF 360

Query: 361 QKKYLDLTRFSACMDFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIA 420
           QK + DLTRF A +DF  G +FL+G+  +  DL NSR P  E+ +   P    S+QQQI 
Sbjct: 361 QKFFFDLTRFHARLDFPHGLRFLTGATSVAQDLLNSRQPSLEAFQKICPEVLVSLQQQIV 420

Query: 421 GPVSFRADTGVAIDLNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELR 471
           GP SF+ ++G+ IDL + G + + V++  FA+EYAL VL SAKA+  YSPK  EFMVELR
Sbjct: 421 GPFSFKVESGIEIDL-RNGANPVTVDKTVFAIEYALQVLLSAKAVVSYSPKQNEFMVELR 479

BLAST of CsaV3_6G000270 vs. TAIR 10
Match: AT3G06960.2 (pigment defective 320 )

HSP 1 Score: 286.2 bits (731), Expect = 4.8e-77
Identity = 148/309 (47.90%), Postives = 212/309 (68.61%), Query Frame = 0

Query: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60
           M ++RW  +G   WDLD+ST  TL+G+A  VP     LPLGLSRG RLSR KQ++F  RF
Sbjct: 1   MNRMRWVGEGD-IWDLDMSTPVTLEGTARAVPD--DPLPLGLSRGTRLSRPKQVEFFHRF 60

Query: 61  MAAPFVPSYSP---------SHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKT-- 120
           MA+P +PS+SP           GFSLQRV ++PFS++  ++LLGQF++Q+F++ + KT  
Sbjct: 61  MASPLIPSFSPIRPNTGDGGGGGFSLQRVLTLPFSNNWLVSLLGQFDVQRFVTEIDKTKA 120

Query: 121 -GSGEMCQSYSSLLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGY-GDSD-IVRT 180
            G G    + +S L  IG+HL  +SLYA+G  ++ LL PDD+L++S+D Y GD D   R 
Sbjct: 121 FGRGS-SSTVASRLNTIGKHLKDKSLYALGFCSEFLLSPDDTLLLSYDAYKGDLDKNPRA 180

Query: 181 KAVFHRKFLHHDLTVEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNA 240
           KA+F+ +F  H+LT EA+ PGLF++K G YWDVP S+ +DL S+ ++SG SYHL +H N+
Sbjct: 181 KAIFNHEFPLHNLTAEAVWPGLFVDKHGEYWDVPLSMAIDLASLPAESGPSYHLCLHHNS 240

Query: 241 GFPSQLGSEPTHSAPFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVS 296
           G P +L S+     P  LLPGLS K+A +++ N ++WR    KL+  +PYD+FLS+PHV+
Sbjct: 241 GSPKKLHSDTMEVPPPSLLPGLSLKSAVSYRTNMDLWRGTTPKLETCKPYDVFLSSPHVA 300

BLAST of CsaV3_6G000270 vs. TAIR 10
Match: AT2G44640.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion, chloroplast, plasma membrane, plastid, chloroplast envelope; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3769 (InterPro:IPR022244); BEST Arabidopsis thaliana protein match is: pigment defective 320 (TAIR:AT3G06960.1); Has 49 Blast hits to 48 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 48; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 231.9 bits (590), Expect = 1.1e-60
Identity = 155/486 (31.89%), Postives = 243/486 (50.00%), Query Frame = 0

Query: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60
           M  L  A+D   FWD +VS+ +TL+G+A  VP      PL  +R  R  R +Q+  ++  
Sbjct: 1   MANLNSAIDSV-FWDQNVSSPQTLEGTARSVPG--EPFPLDGARASRSHRIQQLSLLREG 60

Query: 61  MAAPFVPSYSPSH-----GFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMC 120
                +PS +P+       FSL  +   P S++  + L+GQF  +K  + + K       
Sbjct: 61  FPLGIIPSLAPASDKRLGSFSLNSLLLSPSSNNWWLGLVGQFKPKKLFADI-KADISNAE 120

Query: 121 QSYSSLLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFL 180
           +    +++   +H+  +SLY++G+   I L    SL++S +  GD + +R K +      
Sbjct: 121 EWDLQVVKDTAKHIVDKSLYSIGLWTQIALGTSSSLLLSTERLGDKNGLRNKLMLVHPLE 180

Query: 181 HHDLTVEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQL--- 240
            HDLTVEA  P LF++  GR+WDVP SL VD+ S+  +SG+ Y   +H++ G P  +   
Sbjct: 181 KHDLTVEAAWPDLFLDNKGRFWDVPESLNVDVSSLVPESGVRYRFGLHKSRGNPQPVNAA 240

Query: 241 GSEPTHSAPFCLLPGLSAKAAFAFKKNFEIWRSNAKK-------LKMVQPYDIFLSTPHV 300
           G E    AP  L+PGL AKAA ++K N ++WR   K+         +  PYD+ L  PH 
Sbjct: 241 GVESGSDAPTSLMPGLCAKAAVSYKVNRDLWRPQEKEGNTEEEDKPVFLPYDLRLKEPHA 300

Query: 301 SLSAIIGAVATSYFGDDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGM 360
           ++S I+G+   ++                 +G  +   + RS + AD+F S  ++ Q G 
Sbjct: 301 AISGIVGSSLAAWITG--------------RGMLVNGKK-RSPISADVFGSACYTFQKGR 360

Query: 361 FQKKYLDLTRFSACMDFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATL--PNARFSIQQ 420
           F K Y DLTR  A +D  S   F     L         H  + +   TL  P      QQ
Sbjct: 361 FSKLYGDLTRVDARVDLPSA--FALAKKLF--------HASSNNSDDTLWSPRLNLIFQQ 420

Query: 421 QIAGPVSFRADTGVAIDLNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMV 470
           Q+AGP+ F+ D+   +          R+E+  ++L Y+L +L S K +AWYSPK +E M+
Sbjct: 421 QVAGPIVFKVDSQFQV-------GAARMEDVIYSLNYSLRLLESGKIVAWYSPKRKEGMI 450

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004138517.25.0e-270100.00protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic isoform X1 [Cucumis sativus... [more]
TYK02989.11.6e-25293.83protein TRIGALACTOSYLDIACYLGLYCEROL 4 [Cucumis melo var. makuwa][more]
XP_008458282.11.6e-25293.83PREDICTED: protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucumis melo][more]
XP_038875869.14.2e-23286.17protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Benincasa hispida] >XP_038... [more]
XP_023548884.11.6e-22383.80protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
Q9M9032.9e-11947.31Protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A0A0K8242.4e-270100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G001740 PE=4 SV=1[more]
A0A5D3BY407.9e-25393.83Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3C8377.9e-25393.83protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Cucumis melo OX=3656 GN=... [more]
A0A6J1H3U02.3e-22082.30protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Cucurbita moschata OX=36... [more]
A0A6J1KW751.2e-21982.30protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Cucurbita maxima OX=3661... [more]
Match NameE-valueIdentityDescription
AT3G06960.12.1e-12047.31pigment defective 320 [more]
AT3G06960.24.8e-7747.90pigment defective 320 [more]
AT2G44640.11.1e-6031.89FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR34954:SF4PROTEIN TRIGALACTOSYLDIACYLGLYCEROL 4, CHLOROPLASTICcoord: 1..470
IPR044160Protein TRIGALACTOSYLDIACYLGLYCEROL 4-likePANTHERPTHR34954EXPRESSED PROTEINcoord: 1..470

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_6G000270.1CsaV3_6G000270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034196 acylglycerol transport
biological_process GO:1990052 ER to chloroplast lipid transport
molecular_function GO:0070300 phosphatidic acid binding