CsaV3_5G035990 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_5G035990
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionMajor facilitator superfamily protein
Locationchr5: 28460136 .. 28462503 (-)
RNA-Seq ExpressionCsaV3_5G035990
SyntenyCsaV3_5G035990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCATTCACAACCCTTCAATAAAACCAACCTTCTTATCACTTTCTCTTTCTCTCTTACGCTCTTCTTTCTTTCTTTCAAAAGAGAAAACAAAACCCTCAAAATCTCCCACTTTTGTTTTCTTCATAAAACAATAATCCCCACTTCCAAAAATGGTGGCCGACCGGAGAATCTCTGGTTGCTCTGACATAAGTAGCATCGGGGTCCACGTAATCACTGGACGGTGGTTCGTGGTGTTCGCTTCACTCCTAATCATGGCAGCGGCCGGAGCGACGTACATGTTCGGCTTGTACTCAAGCGACATAAAATCTGTTTTGGGTTACGACCAAACGACGTTGAATTTGCTGAGTTTTTTCAAGGACTTAGGTGCAAACGTCGGCGTTTTGTCTGGACTTATTAACGAGGTTACGCCGCCATGGGTGGTTCTATCTATCGGCGCCGTATTGAACTTCTTTGGTTACTTTATGATTTGGCTTGCCGTTACTCGTCGGATCTCTGCGCCTAAAGTTTGGCAGATGTGTCTCTATATTTGTATTGGAGCCAATTCTCAATCCTTTGCAAATACGGGTTCTCTCGTCACTTGTGTTAAGAACTTCCCTGAAAGCCGTGGAGTCGTTCTTGGTATATGTTTTATTCTTTAAATTATTAAATGTTGGACACACACATTTAAATTTCAAACTTAGGTTTTGTAAATGAATATTATTTAATCTTTAATTATATATTATTGAAATTCAAACTTAGGTTTTGTAAATAAAGAAGAAAAAAAAAAAGACTTCTATTGTTTAGTATTTTCCTGTAGATTCCAACGTTTGATTCCATATGTTTAAACAAAATCAATCACTACTAATTATTGAAACTTCAACGTTACAAAATTTTAGGTATCTTGAAGGGATACGTCGGGCTGAGCGGTGCCATTATCACACAACTCTTCCACGCATTCTACGGCGACGACACCAAATCCTTAATTCTCCTCATTGGATGGCTTCCGGCGGCCATTTCCTTCGCATCTTTACGCACAATTCGTATCATGAAAGTCATCCGTCAACCAAACGAGCTCAAAGTCTTCTACAACTTCCTCTACATTTCTCTTGCCCTCGCCGGATTCCTAATGCTAATGATCATTGTAGAAAGCAAAAAACAGTTCAATCAAAACGAATACGGTGGCAGCGCCGCCGTCGTCCTCCTTCTCCTCATCCTCCCACTTGCTGTAGTCATCATCGAAGAGTACAATCTCTGGAAACTCAAAACCGCTGTCATTAAATCCCCGAATCCCTCTGTTCAAATCGTAACAGAGAAACTCCCAAAAACAGAGCATCCAAAACAAGAGCGAAAAGAACCATCTTGTTGGACAACAATTTTCAGCCCACCACAACGTGGCGAGGATTTCACAATTCTTCAAGCTCTCTTCAGCGTCGACATGCTGATTCTATTTATCGCTGCAATCTGCGGTGTCGGTGGAACGTTAACAGCGATTGACAATTTGGGTCAAATCGGATTGGCTTTGGGATACCCAAAAAGAAGCATTAGCACATTTGTATCATTAGTCAGCATTTGGAATTACCTCGGTCGAGTTGCCTCTGGTTTCATCTCTGAAATTGTTTTGACAAAATACAAATTCCCTCGTCCTTTAATACTCTCTCTAACCCTTCTTCTGTCCTGTGTCGGCCATTTGATGATCGCATTTGACGTCCCAAATGGGTTATATGTAGCTTCCATCGTAATCGGATTCTGCTTTGGTGCACAATGGCCGTTGATTTTCGCCATAATTTCAGAGCTTTTTGGGTTGAAATATTACTCAACGCTTTACAATTTCGGGTCGGTCGCGAGTCCAATTGGGCTATATGTTCTGAATGTGAAAGTGGCTGGGAATTTCTATGACAGAGAAGCAGAGAAGCAGCTCGAAGCAAAAAGGATTATACGAAAAGCAGGGGAAGAATTAAAGTGCTTTGGAGGGGAATGCTTTAAGCTTTCATTCATCGTAATCACAGGAGTTACTTTATTGGGTATGTTAGTTTCATTGATTTTAGTAATTAGAACAAGAAGTTTCTACAAAAGTGATATTTATAAGAAGTTCAGAGATGAAGTTGAAACAACAGAGGTGGCCGGAAATGGGGTTGTAGAGGCCGCCGGTGTACCAGAAGAGAGAAGAAAATGATGGGAATGTAGAAAAGACAAAGGGAAAATTAAAATGTATGAGTAAAATCCAAATGGGTTGAAGTAGAGAGATGAGAATGATAGAATTAAGACAATTTTAGGTTGATTTTTGGGTTTTCTTTTACTCATCTCTTGGCTTTTGTTTGAAAGCACAAAGTTTATCCTTTTGTGAATAAGTTTATAAGCAAAAGAAAAAGAAACAAAGAGTTAAGATAA

mRNA sequence

ATGGTGGCCGACCGGAGAATCTCTGGTTGCTCTGACATAAGTAGCATCGGGGTCCACGTAATCACTGGACGGTGGTTCGTGGTGTTCGCTTCACTCCTAATCATGGCAGCGGCCGGAGCGACGTACATGTTCGGCTTGTACTCAAGCGACATAAAATCTGTTTTGGGTTACGACCAAACGACGTTGAATTTGCTGAGTTTTTTCAAGGACTTAGGTGCAAACGTCGGCGTTTTGTCTGGACTTATTAACGAGGTTACGCCGCCATGGGTGGTTCTATCTATCGGCGCCGTATTGAACTTCTTTGGTTACTTTATGATTTGGCTTGCCGTTACTCGTCGGATCTCTGCGCCTAAAGTTTGGCAGATGTGTCTCTATATTTGTATTGGAGCCAATTCTCAATCCTTTGCAAATACGGGTTCTCTCGTCACTTGTGTTAAGAACTTCCCTGAAAGCCGTGGAGTCGTTCTTGGTATCTTGAAGGGATACGTCGGGCTGAGCGGTGCCATTATCACACAACTCTTCCACGCATTCTACGGCGACGACACCAAATCCTTAATTCTCCTCATTGGATGGCTTCCGGCGGCCATTTCCTTCGCATCTTTACGCACAATTCGTATCATGAAAGTCATCCGTCAACCAAACGAGCTCAAAGTCTTCTACAACTTCCTCTACATTTCTCTTGCCCTCGCCGGATTCCTAATGCTAATGATCATTGTAGAAAGCAAAAAACAGTTCAATCAAAACGAATACGGTGGCAGCGCCGCCGTCGTCCTCCTTCTCCTCATCCTCCCACTTGCTGTAGTCATCATCGAAGAGTACAATCTCTGGAAACTCAAAACCGCTGTCATTAAATCCCCGAATCCCTCTGTTCAAATCGTAACAGAGAAACTCCCAAAAACAGAGCATCCAAAACAAGAGCGAAAAGAACCATCTTGTTGGACAACAATTTTCAGCCCACCACAACGTGGCGAGGATTTCACAATTCTTCAAGCTCTCTTCAGCGTCGACATGCTGATTCTATTTATCGCTGCAATCTGCGGTGTCGGTGGAACGTTAACAGCGATTGACAATTTGGGTCAAATCGGATTGGCTTTGGGATACCCAAAAAGAAGCATTAGCACATTTGTATCATTAGTCAGCATTTGGAATTACCTCGGTCGAGTTGCCTCTGGTTTCATCTCTGAAATTGTTTTGACAAAATACAAATTCCCTCGTCCTTTAATACTCTCTCTAACCCTTCTTCTGTCCTGTGTCGGCCATTTGATGATCGCATTTGACGTCCCAAATGGGTTATATGTAGCTTCCATCGTAATCGGATTCTGCTTTGGTGCACAATGGCCGTTGATTTTCGCCATAATTTCAGAGCTTTTTGGGTTGAAATATTACTCAACGCTTTACAATTTCGGGTCGGTCGCGAGTCCAATTGGGCTATATGTTCTGAATGTGAAAGTGGCTGGGAATTTCTATGACAGAGAAGCAGAGAAGCAGCTCGAAGCAAAAAGGATTATACGAAAAGCAGGGGAAGAATTAAAGTGCTTTGGAGGGGAATGCTTTAAGCTTTCATTCATCGTAATCACAGGAGTTACTTTATTGGGTATGTTAGTTTCATTGATTTTAGTAATTAGAACAAGAAGTTTCTACAAAAGTGATATTTATAAGAAGTTCAGAGATGAAGTTGAAACAACAGAGGTGGCCGGAAATGGGGTTGTAGAGGCCGCCGGTGTACCAGAAGAGAGAAGAAAATGA

Coding sequence (CDS)

ATGGTGGCCGACCGGAGAATCTCTGGTTGCTCTGACATAAGTAGCATCGGGGTCCACGTAATCACTGGACGGTGGTTCGTGGTGTTCGCTTCACTCCTAATCATGGCAGCGGCCGGAGCGACGTACATGTTCGGCTTGTACTCAAGCGACATAAAATCTGTTTTGGGTTACGACCAAACGACGTTGAATTTGCTGAGTTTTTTCAAGGACTTAGGTGCAAACGTCGGCGTTTTGTCTGGACTTATTAACGAGGTTACGCCGCCATGGGTGGTTCTATCTATCGGCGCCGTATTGAACTTCTTTGGTTACTTTATGATTTGGCTTGCCGTTACTCGTCGGATCTCTGCGCCTAAAGTTTGGCAGATGTGTCTCTATATTTGTATTGGAGCCAATTCTCAATCCTTTGCAAATACGGGTTCTCTCGTCACTTGTGTTAAGAACTTCCCTGAAAGCCGTGGAGTCGTTCTTGGTATCTTGAAGGGATACGTCGGGCTGAGCGGTGCCATTATCACACAACTCTTCCACGCATTCTACGGCGACGACACCAAATCCTTAATTCTCCTCATTGGATGGCTTCCGGCGGCCATTTCCTTCGCATCTTTACGCACAATTCGTATCATGAAAGTCATCCGTCAACCAAACGAGCTCAAAGTCTTCTACAACTTCCTCTACATTTCTCTTGCCCTCGCCGGATTCCTAATGCTAATGATCATTGTAGAAAGCAAAAAACAGTTCAATCAAAACGAATACGGTGGCAGCGCCGCCGTCGTCCTCCTTCTCCTCATCCTCCCACTTGCTGTAGTCATCATCGAAGAGTACAATCTCTGGAAACTCAAAACCGCTGTCATTAAATCCCCGAATCCCTCTGTTCAAATCGTAACAGAGAAACTCCCAAAAACAGAGCATCCAAAACAAGAGCGAAAAGAACCATCTTGTTGGACAACAATTTTCAGCCCACCACAACGTGGCGAGGATTTCACAATTCTTCAAGCTCTCTTCAGCGTCGACATGCTGATTCTATTTATCGCTGCAATCTGCGGTGTCGGTGGAACGTTAACAGCGATTGACAATTTGGGTCAAATCGGATTGGCTTTGGGATACCCAAAAAGAAGCATTAGCACATTTGTATCATTAGTCAGCATTTGGAATTACCTCGGTCGAGTTGCCTCTGGTTTCATCTCTGAAATTGTTTTGACAAAATACAAATTCCCTCGTCCTTTAATACTCTCTCTAACCCTTCTTCTGTCCTGTGTCGGCCATTTGATGATCGCATTTGACGTCCCAAATGGGTTATATGTAGCTTCCATCGTAATCGGATTCTGCTTTGGTGCACAATGGCCGTTGATTTTCGCCATAATTTCAGAGCTTTTTGGGTTGAAATATTACTCAACGCTTTACAATTTCGGGTCGGTCGCGAGTCCAATTGGGCTATATGTTCTGAATGTGAAAGTGGCTGGGAATTTCTATGACAGAGAAGCAGAGAAGCAGCTCGAAGCAAAAAGGATTATACGAAAAGCAGGGGAAGAATTAAAGTGCTTTGGAGGGGAATGCTTTAAGCTTTCATTCATCGTAATCACAGGAGTTACTTTATTGGGTATGTTAGTTTCATTGATTTTAGTAATTAGAACAAGAAGTTTCTACAAAAGTGATATTTATAAGAAGTTCAGAGATGAAGTTGAAACAACAGAGGTGGCCGGAAATGGGGTTGTAGAGGCCGCCGGTGTACCAGAAGAGAGAAGAAAATGA

Protein sequence

MVADRRISGCSDISSIGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGDDTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLKTAVIKSPNPSVQIVTEKLPKTEHPKQERKEPSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAIDNLGQIGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVSLILVIRTRSFYKSDIYKKFRDEVETTEVAGNGVVEAAGVPEERRK*
Homology
BLAST of CsaV3_5G035990 vs. NCBI nr
Match: XP_004142499.3 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus] >KAE8648849.1 hypothetical protein Csa_008497 [Cucumis sativus])

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0

Query: 1   MVADRRISGCSDISSIGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60
           MVADRRISGCSDISSIGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT
Sbjct: 1   MVADRRISGCSDISSIGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVW 120
           TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGD 180
           QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGD
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGD 180

Query: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240
           DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE
Sbjct: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLKTAVIKSPNPSVQIVTEKLPKT 300
           SKKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLKTAVIKSPNPSVQIVTEKLPKT
Sbjct: 241 SKKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLKTAVIKSPNPSVQIVTEKLPKT 300

Query: 301 EHPKQERKEPSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAIDNLGQ 360
           EHPKQERKEPSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAIDNLGQ
Sbjct: 301 EHPKQERKEPSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAIDNLGQ 360

Query: 361 IGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGH 420
           IGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGH
Sbjct: 361 IGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGH 420

Query: 421 LMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVL 480
           LMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVL
Sbjct: 421 LMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVL 480

Query: 481 NVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVSLILV 540
           NVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVSLILV
Sbjct: 481 NVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVSLILV 540

Query: 541 IRTRSFYKSDIYKKFRDEVETTEVAGNGVVEAAGVPEERRK 582
           IRTRSFYKSDIYKKFRDEVETTEVAGNGVVEAAGVPEERRK
Sbjct: 541 IRTRSFYKSDIYKKFRDEVETTEVAGNGVVEAAGVPEERRK 581

BLAST of CsaV3_5G035990 vs. NCBI nr
Match: XP_008446819.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo] >KAA0034695.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK09246.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.3e-309
Identity = 552/582 (94.85%), Postives = 566/582 (97.25%), Query Frame = 0

Query: 1   MVADRRISGCSDISSIGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60
           MVA   ISGCSDI SI VHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT
Sbjct: 1   MVAGGGISGCSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVW 120
           TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRIS PKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGD 180
           QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYG+
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGN 180

Query: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240
           DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE
Sbjct: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLKTAVIKSPNPSVQIVTEKLPKT 300
           SKK+FNQNEYGGSAAVVLLLL LPL+VVIIEEYNLWKLKTA++KSPNPSVQIVTEKLPKT
Sbjct: 241 SKKEFNQNEYGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVKSPNPSVQIVTEKLPKT 300

Query: 301 EHPKQ-ERKEPSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAIDNLG 360
           EHPKQ E+KEPSCWTTIF+PPQRGEDFTILQALFS DMLILFIAAICGVGGTLTAIDNLG
Sbjct: 301 EHPKQLEQKEPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIAAICGVGGTLTAIDNLG 360

Query: 361 QIGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVG 420
           QIG++LGYPKRSISTFV+LVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVG
Sbjct: 361 QIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVG 420

Query: 421 HLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYV 480
           HL+IAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLY+
Sbjct: 421 HLIIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYI 480

Query: 481 LNVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVSLIL 540
           LNVKVAGNFYDREAEKQL AK IIRKAGEELKCFGGECFKLSFIVIT VTLLGMLVSL+L
Sbjct: 481 LNVKVAGNFYDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFIVITAVTLLGMLVSLVL 540

Query: 541 VIRTRSFYKSDIYKKFRDEVETTEVAGNGVVEAAGVPEERRK 582
           VIRTRSFYKSDIYKKFRDE ET EVAGN V+E AGVPEERRK
Sbjct: 541 VIRTRSFYKSDIYKKFRDEAETKEVAGNAVIEVAGVPEERRK 582

BLAST of CsaV3_5G035990 vs. NCBI nr
Match: XP_022956604.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 1021.1 bits (2639), Expect = 3.6e-294
Identity = 531/590 (90.00%), Postives = 554/590 (93.90%), Query Frame = 0

Query: 1   MVADRRISGCSDISSIGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60
           MVA   ISGCSDISSI VHVITGRWFVVFASLLIMAAAGATYMF LYSSDIKSVLGYDQT
Sbjct: 1   MVAGGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVW 120
           TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAV+NFFGYFMIWLAVTR+I+APKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTRKIAAPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGD 180
           QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYG 
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGG 180

Query: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240
           DTKSLILLIGWLPAAISFA LRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE
Sbjct: 181 DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLK----TAVIKSPNPSVQIVTEK 300
           SK +F+Q+ YGGSAAVVLLLL LPLAVVIIEEYNLW LK    TA I+SPN  VQIVTEK
Sbjct: 241 SKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAIRSPNHPVQIVTEK 300

Query: 301 LPKTEHPK-----QERKEPSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGT 360
           LPKTEH K     + +KEPSCWTTIF+PP+RGEDFTILQALFSVDMLILF+AAICGVGGT
Sbjct: 301 LPKTEHQKHIPKTEHQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLAAICGVGGT 360

Query: 361 LTAIDNLGQIGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSL 420
           LTAIDNLGQIG++LGYPK+SISTFV+LVSIWNYLGRV SGF+SEIVLTKYKFPRPL+LS+
Sbjct: 361 LTAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSM 420

Query: 421 TLLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSV 480
           TLL+SCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSV
Sbjct: 421 TLLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSV 480

Query: 481 ASPIGLYVLNVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLL 540
           ASPIGLYVLNVKVAGNFYDREAEKQL AK I+RK GEELKCFGGECFKLSF+VITGVTLL
Sbjct: 481 ASPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLL 540

Query: 541 GMLVSLILVIRTRSFYKSDIYKKFRDEVETTEVAGNGVVEAAGVPEERRK 582
           GM VSLILVIRTRSFYKSDIYKKFRDE E TE+AGNG V AAG PEE RK
Sbjct: 541 GMFVSLILVIRTRSFYKSDIYKKFRDEAE-TEMAGNGAVVAAGPPEETRK 589

BLAST of CsaV3_5G035990 vs. NCBI nr
Match: XP_038893353.1 (LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 1018.8 bits (2633), Expect = 1.8e-293
Identity = 522/585 (89.23%), Postives = 550/585 (94.02%), Query Frame = 0

Query: 1   MVADRRISGCSDISSIGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60
           MVA   I GCSD+ SI VHVITGRWFVVFASLLIMAAAGATYMFGLYS DIKSVLGYDQT
Sbjct: 1   MVAGGGILGCSDMRSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSDDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVW 120
           TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGA+LNFFGYFMIWLAVTRRISAPKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAILNFFGYFMIWLAVTRRISAPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGD 180
           QMC+YICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYG+
Sbjct: 121 QMCVYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGN 180

Query: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240
           DTKSLILLIGWLPAAISFA LRTIRI+KVIR PNELKVFYNFLYISL LAGFLMLMII+E
Sbjct: 181 DTKSLILLIGWLPAAISFAFLRTIRIIKVIRHPNELKVFYNFLYISLGLAGFLMLMIIIE 240

Query: 241 SKKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLKTAVIKSPNPSVQIVTEKLPKT 300
           SK +F QNEYGGSAAVVL+LL LPLAVVIIEEYNLWKLKTA IKS NP VQIVTE+LPKT
Sbjct: 241 SKTEFTQNEYGGSAAVVLVLLFLPLAVVIIEEYNLWKLKTAFIKSSNPCVQIVTEQLPKT 300

Query: 301 EH----PKQERKEPSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAID 360
           EH    PKQE+KEPSCWTT+FSPP+RGEDFTILQALFS+DMLILF++A CGVGGTLTAID
Sbjct: 301 EHPKQTPKQEQKEPSCWTTVFSPPERGEDFTILQALFSIDMLILFLSATCGVGGTLTAID 360

Query: 361 NLGQIGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLS 420
           NLGQIG++LGYPKRSISTFVSLVSIWNYLGR+ASGF+SEIV+TKYKFPRPLILSLTLLLS
Sbjct: 361 NLGQIGMSLGYPKRSISTFVSLVSIWNYLGRIASGFVSEIVVTKYKFPRPLILSLTLLLS 420

Query: 421 CVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIG 480
           CVGHLMIAFDVPNGLYVAS+VIGFCFGAQWP+IFAIISELFGLKYYSTLYNFGSVASPIG
Sbjct: 421 CVGHLMIAFDVPNGLYVASVVIGFCFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIG 480

Query: 481 LYVLNVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVS 540
           LY+LNVKVAGNFYDREAEKQL AK I RKAG++LKCFGGECFKLSFIVIT VTLLG  +S
Sbjct: 481 LYILNVKVAGNFYDREAEKQLRAKGITRKAGKKLKCFGGECFKLSFIVITAVTLLGTFIS 540

Query: 541 LILVIRTRSFYKSDIYKKFRDEVETTEVAGNGVVEAAGVPEERRK 582
           LILVIRT+SFYKSDIYKKFRDE E TEVAGNG V  AG  EE +K
Sbjct: 541 LILVIRTKSFYKSDIYKKFRDEAE-TEVAGNGAVAPAGAGEETKK 584

BLAST of CsaV3_5G035990 vs. NCBI nr
Match: KAG6601185.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1018.5 bits (2632), Expect = 2.4e-293
Identity = 527/590 (89.32%), Postives = 554/590 (93.90%), Query Frame = 0

Query: 1   MVADRRISGCSDISSIGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60
           M A   I+GCSDISSI VH+ITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT
Sbjct: 1   MAAGGGIAGCSDISSIAVHIITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVW 120
           TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGA++NFFGYFMIWLAVT++I+APKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAIMNFFGYFMIWLAVTQKIAAPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGD 180
           QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYG 
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGG 180

Query: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240
           DTKSLILLIGWLPAAISFA LRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE
Sbjct: 181 DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLK----TAVIKSPNPSVQIVTEK 300
           SK +F+Q+ YGGSAAVVLLLL LPLAVVIIEEYNLW LK    TA I+SPN  VQIVTEK
Sbjct: 241 SKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAIRSPNHPVQIVTEK 300

Query: 301 LPKTEHPK-----QERKEPSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGT 360
           LPKTEH K     + +KEPSCWTTIF+PP+RGEDFTILQALFSVDMLILF+AAICGVGGT
Sbjct: 301 LPKTEHQKHIPKTEHQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLAAICGVGGT 360

Query: 361 LTAIDNLGQIGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSL 420
           LTAIDNLGQIG++LGYPK+SISTFV+LVSIWNYLGRV SGF+SEIVLTKYKFPRPL+LS+
Sbjct: 361 LTAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSM 420

Query: 421 TLLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSV 480
           TLL+SCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSV
Sbjct: 421 TLLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSV 480

Query: 481 ASPIGLYVLNVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLL 540
           ASPIGLYVLNVKVAGNFYDREAEKQL AK I+RK GEELKCFGGECFKLSF+VITGVTLL
Sbjct: 481 ASPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLL 540

Query: 541 GMLVSLILVIRTRSFYKSDIYKKFRDEVETTEVAGNGVVEAAGVPEERRK 582
           GM VSLILVIRTRSFYKSDIYKKFRDE E TE+AGNG V AAG PEE RK
Sbjct: 541 GMFVSLILVIRTRSFYKSDIYKKFRDEAE-TEMAGNGAVVAAGPPEETRK 589

BLAST of CsaV3_5G035990 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 9.1e-30
Identity = 133/536 (24.81%), Postives = 246/536 (45.90%), Query Frame = 0

Query: 24  RWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLIN 83
           +W V+ A++ I A+ G  + F  YSS +KSVLG  Q  LN L+   DLG   G  SG+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 84  EVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVWQMCLYICIGAN-SQSFANTGSLV 143
              P  VVL   A + F GY + WL +T  I+ P  + +    C+ A  S  + NT   +
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP--YSLVFLCCLLAGLSICWFNTACFI 162

Query: 144 TCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGDDTKSLILLIGWLPAAISFASLR 203
            C+++FP +R + L +   + G+S A+ +  F+A     +   +LL   +P  +SFA+L 
Sbjct: 163 LCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALY 222

Query: 204 TIRIMKVI-------RQPNELKVFYNFLYISLALAGFLMLMIIVESKKQFNQNEYGGSAA 263
            +     +        + ++  VF     +++  +  L+L     S  + N   + G+  
Sbjct: 223 PVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLN---FIGAVV 282

Query: 264 VVLLLLILPLAVVIIEEYNLWKLKTAVIKSPNPSVQIVTEKL--PKTEHPKQERKEPSCW 323
           +++  L  PL +V   +Y L  +   +    +  V +  ++L   KT    +   E    
Sbjct: 283 LLVFPLCAPL-LVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGT 342

Query: 324 TTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAIDNLGQIGLALGYPKRSIS 383
               +  + G++ +    +  ++  + +IA  CG    L   +NLGQI  +LG   ++ +
Sbjct: 343 AKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG---QNST 402

Query: 384 TFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGHLMIAF--DVPNGL 443
           T V++ S +++ GR+ S    + +  +++  R    ++ LL + +   ++A        L
Sbjct: 403 TLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTAL 462

Query: 444 YVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDR 503
             A+ +IG   G  +    +I S+LFG       +N      PIG  +L   +A + Y+ 
Sbjct: 463 QTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIG-SLLYGYIAASIYEA 522

Query: 504 EAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVSLILVIRTRSFY 548
            A        I     + + C G +C+  +F+    +++LG++ SL L IRT+  Y
Sbjct: 523 NASPD-----ITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of CsaV3_5G035990 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.0e-05
Identity = 57/229 (24.89%), Postives = 100/229 (43.67%), Query Frame = 0

Query: 323 GEDFTILQALFSVDMLILFIAAICGVGGTLTAI-DNLGQI---GLALGYPKRSISTFVSL 382
           G +   L+  F+     LF+     +GG      +N+G I            S ST VSL
Sbjct: 259 GHEGATLKEFFTDKTAWLFLLCFVFIGGPFEMFQNNMGAILDTVTVENADSPSFSTHVSL 318

Query: 383 VSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGHLMI------AFDVPNGLY 442
            + ++ + R+  GF SE +  +    RP++LS+  L++   HLM+       FD      
Sbjct: 319 FATFSTVSRLVVGFSSEAM--ESHVSRPVLLSVIALVAACIHLMVPSGIFTVFDNAKYFS 378

Query: 443 VASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDRE 502
           V +IV GF +G+ + L+  I+++++G+    T++         G ++L + V    Y   
Sbjct: 379 VVTIVNGFSYGSSFTLVPTIVTKVWGIANLGTIW---------GSFILALAVGSLGYGLL 438

Query: 503 AEKQLEAKRIIRKAGEELKCFGGECFKLSFIVI-TGVTLLGMLVSLILV 541
             K  +A   +        C G  C+ L+F++  TG+      V  I V
Sbjct: 439 FAKVYDAASEVGVGSMSQVCSGVHCYGLTFVITGTGLAFAAAAVFFIWV 476

BLAST of CsaV3_5G035990 vs. ExPASy TrEMBL
Match: A0A5D3CFW3 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002400 PE=4 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 6.5e-310
Identity = 552/582 (94.85%), Postives = 566/582 (97.25%), Query Frame = 0

Query: 1   MVADRRISGCSDISSIGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60
           MVA   ISGCSDI SI VHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT
Sbjct: 1   MVAGGGISGCSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVW 120
           TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRIS PKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGD 180
           QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYG+
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGN 180

Query: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240
           DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE
Sbjct: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLKTAVIKSPNPSVQIVTEKLPKT 300
           SKK+FNQNEYGGSAAVVLLLL LPL+VVIIEEYNLWKLKTA++KSPNPSVQIVTEKLPKT
Sbjct: 241 SKKEFNQNEYGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVKSPNPSVQIVTEKLPKT 300

Query: 301 EHPKQ-ERKEPSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAIDNLG 360
           EHPKQ E+KEPSCWTTIF+PPQRGEDFTILQALFS DMLILFIAAICGVGGTLTAIDNLG
Sbjct: 301 EHPKQLEQKEPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIAAICGVGGTLTAIDNLG 360

Query: 361 QIGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVG 420
           QIG++LGYPKRSISTFV+LVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVG
Sbjct: 361 QIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVG 420

Query: 421 HLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYV 480
           HL+IAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLY+
Sbjct: 421 HLIIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYI 480

Query: 481 LNVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVSLIL 540
           LNVKVAGNFYDREAEKQL AK IIRKAGEELKCFGGECFKLSFIVIT VTLLGMLVSL+L
Sbjct: 481 LNVKVAGNFYDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFIVITAVTLLGMLVSLVL 540

Query: 541 VIRTRSFYKSDIYKKFRDEVETTEVAGNGVVEAAGVPEERRK 582
           VIRTRSFYKSDIYKKFRDE ET EVAGN V+E AGVPEERRK
Sbjct: 541 VIRTRSFYKSDIYKKFRDEAETKEVAGNAVIEVAGVPEERRK 582

BLAST of CsaV3_5G035990 vs. ExPASy TrEMBL
Match: A0A1S3BG09 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 PE=4 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 6.5e-310
Identity = 552/582 (94.85%), Postives = 566/582 (97.25%), Query Frame = 0

Query: 1   MVADRRISGCSDISSIGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60
           MVA   ISGCSDI SI VHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT
Sbjct: 1   MVAGGGISGCSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVW 120
           TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRIS PKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGD 180
           QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYG+
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGN 180

Query: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240
           DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE
Sbjct: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLKTAVIKSPNPSVQIVTEKLPKT 300
           SKK+FNQNEYGGSAAVVLLLL LPL+VVIIEEYNLWKLKTA++KSPNPSVQIVTEKLPKT
Sbjct: 241 SKKEFNQNEYGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVKSPNPSVQIVTEKLPKT 300

Query: 301 EHPKQ-ERKEPSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAIDNLG 360
           EHPKQ E+KEPSCWTTIF+PPQRGEDFTILQALFS DMLILFIAAICGVGGTLTAIDNLG
Sbjct: 301 EHPKQLEQKEPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIAAICGVGGTLTAIDNLG 360

Query: 361 QIGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVG 420
           QIG++LGYPKRSISTFV+LVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVG
Sbjct: 361 QIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVG 420

Query: 421 HLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYV 480
           HL+IAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLY+
Sbjct: 421 HLIIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYI 480

Query: 481 LNVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVSLIL 540
           LNVKVAGNFYDREAEKQL AK IIRKAGEELKCFGGECFKLSFIVIT VTLLGMLVSL+L
Sbjct: 481 LNVKVAGNFYDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFIVITAVTLLGMLVSLVL 540

Query: 541 VIRTRSFYKSDIYKKFRDEVETTEVAGNGVVEAAGVPEERRK 582
           VIRTRSFYKSDIYKKFRDE ET EVAGN V+E AGVPEERRK
Sbjct: 541 VIRTRSFYKSDIYKKFRDEAETKEVAGNAVIEVAGVPEERRK 582

BLAST of CsaV3_5G035990 vs. ExPASy TrEMBL
Match: A0A6J1GZI8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111458291 PE=4 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 1.8e-294
Identity = 531/590 (90.00%), Postives = 554/590 (93.90%), Query Frame = 0

Query: 1   MVADRRISGCSDISSIGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60
           MVA   ISGCSDISSI VHVITGRWFVVFASLLIMAAAGATYMF LYSSDIKSVLGYDQT
Sbjct: 1   MVAGGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVW 120
           TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAV+NFFGYFMIWLAVTR+I+APKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTRKIAAPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGD 180
           QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYG 
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGG 180

Query: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240
           DTKSLILLIGWLPAAISFA LRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE
Sbjct: 181 DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLK----TAVIKSPNPSVQIVTEK 300
           SK +F+Q+ YGGSAAVVLLLL LPLAVVIIEEYNLW LK    TA I+SPN  VQIVTEK
Sbjct: 241 SKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAIRSPNHPVQIVTEK 300

Query: 301 LPKTEHPK-----QERKEPSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGT 360
           LPKTEH K     + +KEPSCWTTIF+PP+RGEDFTILQALFSVDMLILF+AAICGVGGT
Sbjct: 301 LPKTEHQKHIPKTEHQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLAAICGVGGT 360

Query: 361 LTAIDNLGQIGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSL 420
           LTAIDNLGQIG++LGYPK+SISTFV+LVSIWNYLGRV SGF+SEIVLTKYKFPRPL+LS+
Sbjct: 361 LTAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSM 420

Query: 421 TLLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSV 480
           TLL+SCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSV
Sbjct: 421 TLLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSV 480

Query: 481 ASPIGLYVLNVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLL 540
           ASPIGLYVLNVKVAGNFYDREAEKQL AK I+RK GEELKCFGGECFKLSF+VITGVTLL
Sbjct: 481 ASPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLL 540

Query: 541 GMLVSLILVIRTRSFYKSDIYKKFRDEVETTEVAGNGVVEAAGVPEERRK 582
           GM VSLILVIRTRSFYKSDIYKKFRDE E TE+AGNG V AAG PEE RK
Sbjct: 541 GMFVSLILVIRTRSFYKSDIYKKFRDEAE-TEMAGNGAVVAAGPPEETRK 589

BLAST of CsaV3_5G035990 vs. ExPASy TrEMBL
Match: A0A6J1K186 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489721 PE=4 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.4e-291
Identity = 526/586 (89.76%), Postives = 548/586 (93.52%), Query Frame = 0

Query: 1   MVADRRISGCSDISSIGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60
           MVA   ISGCSDISSI VHVITGRWFVVFASLLIMAAAGATYMF LYSSDIKSVLGYDQT
Sbjct: 1   MVAGGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVW 120
           TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAV+NFFGYFMIWLAVT +ISAPKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTGKISAPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGD 180
           QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYG 
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGG 180

Query: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240
           DTKSLILLIGWLPAAISFA LRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMII+E
Sbjct: 181 DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIE 240

Query: 241 SKKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLKT-----AVIKSPNPSVQIVTE 300
           SK +F+Q+ YGGSAAVVLLLL LPLAVVIIEEYNLW LKT     A I+SPN  VQIVTE
Sbjct: 241 SKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAAIRSPNHPVQIVTE 300

Query: 301 KLPKTEHPKQERKEPSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAI 360
           K+PKTEH KQ    PSCWTTIF+PP+RGEDFTILQALFSVDM ILF+AAICGVGGTLTAI
Sbjct: 301 KVPKTEHQKQ----PSCWTTIFNPPERGEDFTILQALFSVDMFILFLAAICGVGGTLTAI 360

Query: 361 DNLGQIGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLL 420
           DNLGQIG++LGYPK+SISTFV+LVSIWNYLGRV SGF+SEIVLTKYKFPRPL+LS+TLL+
Sbjct: 361 DNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSMTLLV 420

Query: 421 SCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPI 480
           SCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPI
Sbjct: 421 SCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPI 480

Query: 481 GLYVLNVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLV 540
           GLYVLNVKVAGNFYDREAEKQL AK I+RK GEELKCFGGECFKLSF+VITGVTLLGM V
Sbjct: 481 GLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLLGMFV 540

Query: 541 SLILVIRTRSFYKSDIYKKFRDEVETTEVAGNGVVEAAGVPEERRK 582
           SLILVIRTRSFYKSDIYKKFRDE E TE+AGNG V AA  PEE RK
Sbjct: 541 SLILVIRTRSFYKSDIYKKFRDEAE-TEIAGNGAVVAAEPPEETRK 581

BLAST of CsaV3_5G035990 vs. ExPASy TrEMBL
Match: A0A6J1FZF2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111449326 PE=4 SV=1)

HSP 1 Score: 973.8 bits (2516), Expect = 3.2e-280
Identity = 507/581 (87.26%), Postives = 536/581 (92.25%), Query Frame = 0

Query: 4   DRRISGCSDISSIGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLN 63
           DR ISG S+I +I VH+ITGRWFVVFASLLIMAAAGATYMF LYS+DIKSVLGYDQTTLN
Sbjct: 9   DRAISGSSNIRTIAVHIITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVLGYDQTTLN 68

Query: 64  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVWQMC 123
           LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVT+RIS PKVWQMC
Sbjct: 69  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQMC 128

Query: 124 LYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGDDTK 183
           LYIC+GANSQSF NTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYG DTK
Sbjct: 129 LYICVGANSQSFVNTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGSDTK 188

Query: 184 SLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKK 243
           SLILLIGWLPAAISFA LRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMII+ESK 
Sbjct: 189 SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKL 248

Query: 244 QFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLKTAVIKSPNPSVQIVTEK--LPKTE 303
            F+Q E+GGSAAVVLLLL LPLAVVI+EEYNL KLK+A I  PNP +QI+TEK   PKTE
Sbjct: 249 IFSQAEFGGSAAVVLLLLFLPLAVVIVEEYNLSKLKSAAINPPNP-LQILTEKPNPPKTE 308

Query: 304 HPKQER--KEPSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAIDNLG 363
             KQ R  K+PSCWTTIFSPP+RGEDFTILQALFS+DM ILF +AICGVGGTLTAIDNLG
Sbjct: 309 PQKQTRQQKQPSCWTTIFSPPERGEDFTILQALFSLDMAILFFSAICGVGGTLTAIDNLG 368

Query: 364 QIGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVG 423
           QIG++LGYPKRSISTFV+LVSIWNYLGRVASGF+SEIVLTKYKFPRPL+LSLTLLLSCVG
Sbjct: 369 QIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLLSCVG 428

Query: 424 HLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYV 483
           HL+IAF VPNGLYVASIVIGFCFGAQWPL+FAIISELFGLKYYSTLYNFGSVASPIGLYV
Sbjct: 429 HLIIAFAVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFGSVASPIGLYV 488

Query: 484 LNVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVSLIL 543
           LNVKVAGN YD+EA KQL AK ++RK GEELKC+GGECFK SF+VITGVTLLGM VSLIL
Sbjct: 489 LNVKVAGNLYDKEAAKQLAAKGLVRKTGEELKCYGGECFKRSFLVITGVTLLGMFVSLIL 548

Query: 544 VIRTRSFYKSDIYKKFRDEVETTEVAGNGVVEAAGVPEERR 581
           VIRTR FYKSDIYKKFRDE E  EV GNG V AAG PEE R
Sbjct: 549 VIRTRGFYKSDIYKKFRDEAE-AEVVGNGAVTAAGAPEETR 587

BLAST of CsaV3_5G035990 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 807.0 bits (2083), Expect = 1.0e-233
Identity = 408/565 (72.21%), Postives = 473/565 (83.72%), Query Frame = 0

Query: 13  ISSIGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLNLLSFFKDLG 72
           + S+ + ++TGRWF+ F SLLIM+ AGATYMFG+YS DIK  LGYDQTTLNLLSFFKDLG
Sbjct: 10  MKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLG 69

Query: 73  ANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVWQMCLYICIGANS 132
           ANVGVL+GL+NEVTPPW +L IGA+LNFFGYFMIWLAVT RIS P+VW MCLYIC+GANS
Sbjct: 70  ANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANS 129

Query: 133 QSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGDDTKSLILLIGWL 192
           QSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQL+ AFYG+DTK LIL+IGWL
Sbjct: 130 QSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWL 189

Query: 193 PAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKKQFNQNEYGG 252
           PA +SFA LRTIRIMKV RQ NELKVFYNFLYISL LA FLM++II+     F Q+E+GG
Sbjct: 190 PAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGG 249

Query: 253 SAAVVLLLLILPLAVVIIEEYNLWKLKTAVIKSPNPSVQIVTEKLPKTEHPK------QE 312
           SAAVV++LL+LP+ VVI+EE  LWK K   +  P P + +VTEK PK +  +      +E
Sbjct: 250 SAAVVIVLLLLPIIVVILEEKKLWKEKQVALNDPAP-INVVTEK-PKLDSSEFKDDDGEE 309

Query: 313 RKE-------PSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAIDNLG 372
            KE       PSCWTT+F+PP+RG+D+TILQALFSVDMLILF+A ICGVGGTLTAIDNLG
Sbjct: 310 SKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLG 369

Query: 373 QIGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVG 432
           QIG +LGYPKRS+STFVSLVSIWNY GRV SG +SEI L KYKFPRPL+L++ LLLSC G
Sbjct: 370 QIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAG 429

Query: 433 HLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYV 492
           HL+IAF+VP GLYVAS++IGFCFGAQWPL+FAIISE+FGLKYYSTLYNFGSVASPIG Y+
Sbjct: 430 HLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYL 489

Query: 493 LNVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVSLIL 552
           LNV+VAG  YD EA KQ +A    R  G++L C G  CFKLSFI+I  VTL G+LVS++L
Sbjct: 490 LNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVL 549

Query: 553 VIRTRSFYKSDIYKKFRDEVETTEV 565
           VIRT+ FYKSDIYKKFR++    E+
Sbjct: 550 VIRTKKFYKSDIYKKFREKALAAEM 572

BLAST of CsaV3_5G035990 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 685.6 bits (1768), Expect = 3.4e-197
Identity = 348/569 (61.16%), Postives = 430/569 (75.57%), Query Frame = 0

Query: 18  VHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLNLLSFFKDLGANVGV 77
           +H   GRWF+VFAS LIMA AGATY+FG YS DIKS LGYDQTTLNLL FFKDLGANVGV
Sbjct: 11  IHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGV 70

Query: 78  LSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVWQMCLYICIGANSQSFAN 137
           LSGLI EVTP W VL+IG+ +NF GYFMIWL VT +++ PKVWQMCLYICIGANSQ+FAN
Sbjct: 71  LSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFAN 130

Query: 138 TGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGDDTKSLILLIGWLPAAIS 197
           TG+LVTCVKNFPESRGV+LG+LKGYVGLSGAI TQL+ A YG D+KSLILLI WLPAA+S
Sbjct: 131 TGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAAVS 190

Query: 198 FASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKKQFNQNEYGGSAAVV 257
              +  IR  KV+RQ NEL VFY FLYIS+ LA FLM M I E +  F++  Y  SA + 
Sbjct: 191 LVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATIC 250

Query: 258 LLLLILPLAVVIIEEYNLWKLKTAVIKSPN------PSVQIVTEKLPKTEHPKQERKEPS 317
             LL +PL V + +E  +W +    I+ P+      P  ++  ++    +   +E++  S
Sbjct: 251 CALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKETKS 310

Query: 318 CWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAIDNLGQIGLALGYPKRS 377
           C++T+FSPP RGED+TILQAL S DM+ILF+A  CG+G +LTA+DNLGQIG +LGYP  +
Sbjct: 311 CFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHT 370

Query: 378 ISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGHLMIAFDVPNGL 437
           +S+FVSLVSIWNY GRV SGF+SE +L KYK PRPL+++L LLLSC GHL+IAF VP  +
Sbjct: 371 VSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSV 430

Query: 438 YVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDR 497
           Y+ASI++GF FGAQ PL+FAIISELFGLKYYSTL+N G +ASP+G Y+LNV+V G  YD+
Sbjct: 431 YIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYDK 490

Query: 498 EAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVSLILVIRTRSFYKSDI 557
           EA KQL A+ + RK  ++L C G +C+KL F+++  VT  G LVSL L IRTR FYK DI
Sbjct: 491 EALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGDI 550

Query: 558 YKKFRDEVETTEVAGNGVVEAAGVPEERR 581
           YKKFR+  E+         E+  VP+ R+
Sbjct: 551 YKKFRESPES---------ESELVPDSRK 570

BLAST of CsaV3_5G035990 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 343.2 bits (879), Expect = 4.1e-94
Identity = 203/555 (36.58%), Postives = 317/555 (57.12%), Query Frame = 0

Query: 18  VHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLNLLSFFKDLGANVGV 77
           + ++  +W  + AS+ I  A+GA+Y FG+YS+ +KS   YDQ+TL+ +S FKD+GAN GV
Sbjct: 1   MEILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGV 60

Query: 78  LSGL----------------INEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVWQ 137
            SGL                I     PWVVL++GA+  F GYF+IW +VT  I  P V  
Sbjct: 61  FSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPL 120

Query: 138 MCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGDD 197
           MCL++ + A SQ+F NT ++V+ V+NF +  G  +GI+KG++GLSGAI+ QL+      D
Sbjct: 121 MCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD 180

Query: 198 TKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVES 257
             S ILL+   P  +S   +  +RI +     ++ K       +SL +A +LM++II+  
Sbjct: 181 PASFILLLAVTPTVLSLLVMPLVRIYET-SVADDKKHLNGLSAVSLIIAAYLMIIIIL-- 240

Query: 258 KKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLKTAVIKSPNPSVQIVTEKLPKTE 317
           K  F  + +     +V LL++L L ++I        ++  V    +P +       PK  
Sbjct: 241 KNTFGLSSWANIVTLVCLLVMLALPLLIARRAQRDGMEKTVPHDYSPLI-----SSPKAT 300

Query: 318 HPKQERKEPSCWTTIFSPPQRG--EDFTILQALFSVDMLILFIAAICGVGGTLTAIDNLG 377
               +  E        S  + G  E+  +LQA+  +   +LF+A ICG+G  L+ I+N+ 
Sbjct: 301 TSGNQSSEGD------SKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIR 360

Query: 378 QIGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVG 437
           QIG +L Y    I++ VSL SIWN+LGR  +G+ S+ +L K  +PRPL+++ TL    +G
Sbjct: 361 QIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIG 420

Query: 438 HLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYV 497
           HL+IA      LYV S+++G C+G+QW L+  I SELFG+++  T++N  SVASPIG Y+
Sbjct: 421 HLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYI 480

Query: 498 LNVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVSLIL 555
            +V++ G  YD+ A            +GE   C+G  CF+LSFI++  V   G LV+++L
Sbjct: 481 FSVRLIGYIYDKTA------------SGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVL 529

BLAST of CsaV3_5G035990 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 339.0 bits (868), Expect = 7.7e-93
Identity = 200/553 (36.17%), Postives = 318/553 (57.50%), Query Frame = 0

Query: 16  IGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLNLLSFFKDLGANV 75
           +   ++  +W  + AS+ I  +AG +Y FG+YS+ +KS   YDQ+TL+ +S FKD+G NV
Sbjct: 1   MATEILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNV 60

Query: 76  GVLSGLINEVTP--------------PWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVWQ 135
           GVLSGL+                   PWVV+ IGA+LNF GYF++W +VT  I  P V  
Sbjct: 61  GVLSGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPV 120

Query: 136 MCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGDD 195
           MCL++ I A S +F NT ++V+ ++NF +  G  +GI+KG+VGLSGA++ QL+      D
Sbjct: 121 MCLFMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGD 180

Query: 196 TKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVES 255
            K+ ILL+  +P+ +S   +  +R+ K     +E K       +SL +A +LM+ II++S
Sbjct: 181 PKTFILLLAIVPSLLSVLVMPLVRVYKT-STVDEKKHLDGLSTLSLIIAAYLMITIILKS 240

Query: 256 KKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLKTAVIKSPNPSVQIVTEKLPKTE 315
                      + AV+L+LL  PL V               +++   S++   + L    
Sbjct: 241 TLSLPSWANAVTLAVLLVLLSSPLLV--------------AVRAHRDSIE---KPLSSVY 300

Query: 316 HPKQERKEPSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAIDNLGQI 375
            P  +  E +    I    +  +   +LQA+ +VD  +LF+A ICG+G  ++ I+N+ QI
Sbjct: 301 SPLVDNLEATTSGEILMLDE-DKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQI 360

Query: 376 GLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGHL 435
           G +L Y    I++ ++L +IWN++GR   G++S+ +L +  +PRPL+++ TL    +GHL
Sbjct: 361 GESLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHL 420

Query: 436 MIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVLN 495
           +IA      LY  SI++G C+G+QW L+  I SELFG+K+  T+YN  S+ASP+G Y+ +
Sbjct: 421 IIASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFS 480

Query: 496 VKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVSLILVI 555
           V++ G  YDR               GE   C+G  CF+L+++VI  V  LG LVS +LV 
Sbjct: 481 VRLIGYIYDRTI------------IGEGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVF 521

BLAST of CsaV3_5G035990 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 335.5 bits (859), Expect = 8.5e-92
Identity = 208/576 (36.11%), Postives = 323/576 (56.08%), Query Frame = 0

Query: 18  VHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLNLLSFFKDLGANVGV 77
           V  I  RW V  A++ I + AG  Y+FG  S  IKS L Y+Q  L+ L   KDLG +VG 
Sbjct: 10  VSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGF 69

Query: 78  LSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVWQMCLYICIGANSQSFAN 137
           ++G ++E+ P W  L +GAV N  GY  +WL VT R     +W MC+ I +G N +++ N
Sbjct: 70  IAGTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFN 129

Query: 138 TGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGDDTKSLILLIGWLPAAIS 197
           TG+LV+ V+NFP+SRG V+GILKG+ GL GAII+Q++   +  +  SLIL++   PA + 
Sbjct: 130 TGALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVV 189

Query: 198 FASLRTIRIM---KVIRQPNELKVFYNFLY-ISLALAGFLMLMIIVESKKQFNQNEYGGS 257
              +  IR +   K IR  +     + F+Y + L LA +LM +++++     + N     
Sbjct: 190 VCLMFFIRPVGGHKQIRPTDGAS--FTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVF 249

Query: 258 AAVVLLLLILPLAVVI--------------IEEYNLWKLKTAVIKSPNPSV---QIVTEK 317
             V+ ++L++P+ V I              IEE  + K +        P +   ++  EK
Sbjct: 250 TIVLFVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEK 309

Query: 318 ------LPKTEHPK----------QERKEPSCWTTIFSPPQRGEDFTILQALFSVDMLIL 377
                 LP +E  K          Q   E +        P RGEDFT+ QAL   D  ++
Sbjct: 310 PKDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLI 369

Query: 378 FIAAICGVGGTLTAIDNLGQIGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTK 437
           F + + G G  LT IDNLGQ+  +LGY    +   VS++SIWN+LGR+  G+ SE+V+  
Sbjct: 370 FFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHV--LVSMISIWNFLGRIGGGYFSELVVRD 429

Query: 438 YKFPRPLILSLTLLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLK 497
           Y +PRP+ +++  L+  VGH+  A+  P  +Y+ +++IG  +GA W ++ A  SELFGLK
Sbjct: 430 YAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLK 489

Query: 498 YYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKL 557
            +  LYNF ++A+P G  V +  +A + YDREAE+Q  A   +    + L+C G  CF L
Sbjct: 490 KFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQ--AHGSVFDPDDALRCNGSICFFL 549

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004142499.30.0e+00100.00protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus] >KAE8648849.1 hypothet... [more]
XP_008446819.11.3e-30994.85PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo] >KAA0034695.1 ... [more]
XP_022956604.13.6e-29490.00protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata][more]
XP_038893353.11.8e-29389.23LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida][more]
KAG6601185.12.4e-29389.32Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
F4I9E19.1e-3024.81Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q6CGU82.0e-0524.89Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28... [more]
Match NameE-valueIdentityDescription
A0A5D3CFW36.5e-31094.85Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3BG096.5e-31094.85protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 ... [more]
A0A6J1GZI81.8e-29490.00protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1K1861.4e-29189.76protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489... [more]
A0A6J1FZF23.2e-28087.26protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111449326... [more]
Match NameE-valueIdentityDescription
AT2G39210.11.0e-23372.21Major facilitator superfamily protein [more]
AT2G28120.13.4e-19761.16Major facilitator superfamily protein [more]
AT1G74780.14.1e-9436.58Nodulin-like / Major Facilitator Superfamily protein [more]
AT1G18940.17.7e-9336.17Nodulin-like / Major Facilitator Superfamily protein [more]
AT5G14120.18.5e-9236.11Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 334..548
e-value: 1.7E-9
score: 39.3
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 24..545
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 24..269
e-value: 1.3E-95
score: 319.6
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 9..564
NoneNo IPR availablePANTHERPTHR21576:SF117MFS TRANSPORTERcoord: 9..564
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 23..533
e-value: 5.66004E-119
score: 355.791

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_5G035990.1CsaV3_5G035990.1mRNA