Homology
BLAST of CsaV3_5G035110 vs. NCBI nr
Match:
XP_011655823.1 (steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN52160.1 hypothetical protein Csa_009086 [Cucumis sativus])
HSP 1 Score: 532.3 bits (1370), Expect = 2.4e-147
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0
Query: 1 MQFTAAMNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSAT 60
MQFTAAMNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSAT
Sbjct: 1 MQFTAAMNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSAT 60
Query: 61 KSSNSEQLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVL 120
KSSNSEQLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVL
Sbjct: 61 KSSNSEQLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVL 120
Query: 121 FVHKYSGKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGN 180
FVHKYSGKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGN
Sbjct: 121 FVHKYSGKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGN 180
Query: 181 MYHHILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIAT 240
MYHHILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIAT
Sbjct: 181 MYHHILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIAT 240
Query: 241 AIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF 275
AIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF
Sbjct: 241 AIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF 274
BLAST of CsaV3_5G035110 vs. NCBI nr
Match:
XP_008446723.1 (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo])
HSP 1 Score: 491.5 bits (1264), Expect = 4.7e-135
Identity = 250/268 (93.28%), Postives = 257/268 (95.90%), Query Frame = 0
Query: 7 MNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSATKSSNSE 66
MN LS FFFP+PPSLLVNVMTVVSS VVAVIG SEARGTHLQYSKFWNTSNSATKSSNSE
Sbjct: 1 MNALSVFFFPMPPSLLVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTSNSATKSSNSE 60
Query: 67 QLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVLFVHKYS 126
QLRISSRLGMLLLYTPA LAGAASFWF PNDD RVLLLKSALT HFFKRDFEVLFVHKYS
Sbjct: 61 QLRISSRLGMLLLYTPAALAGAASFWFFPNDDWRVLLLKSALTLHFFKRDFEVLFVHKYS 120
Query: 127 GKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGNMYHHIL 186
GKMV+DSA IISLSYFSSTALMIYAQQ SKALPEPTIDLKNIGIA+F+IGIIGNMYHHIL
Sbjct: 121 GKMVLDSASIISLSYFSSTALMIYAQQLSKALPEPTIDLKNIGIALFLIGIIGNMYHHIL 180
Query: 187 LSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIATAIYLGG 246
LSQTRKKGETGYKIPKGGLFGI+ICPHY+FEITVYFGFALISQTFY+FFFAIATAIYLGG
Sbjct: 181 LSQTRKKGETGYKIPKGGLFGIVICPHYLFEITVYFGFALISQTFYSFFFAIATAIYLGG 240
Query: 247 RSYATRKWYVSKFEDFPQHIKALIPFVF 275
RSYAT KWYVSKFEDFPQHIKALIPFVF
Sbjct: 241 RSYATWKWYVSKFEDFPQHIKALIPFVF 268
BLAST of CsaV3_5G035110 vs. NCBI nr
Match:
KAA0034632.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo var. makuwa] >TYK09183.1 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo var. makuwa])
HSP 1 Score: 475.7 bits (1223), Expect = 2.7e-130
Identity = 242/258 (93.80%), Postives = 249/258 (96.51%), Query Frame = 0
Query: 17 LPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSATKSSNSEQLRISSRLGM 76
+PPSLLVNVMTVVSS VVAVIG SEARGTHLQYSKFWNTSNSATKSSNSEQLRISSRLGM
Sbjct: 1 MPPSLLVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTSNSATKSSNSEQLRISSRLGM 60
Query: 77 LLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVLFVHKYSGKMVIDSALI 136
LLLYTPA LAGAASFWF PNDD RVLLLKSALT HFFKRDFEVLFVHKYSGKMV+DSA I
Sbjct: 61 LLLYTPAALAGAASFWFFPNDDWRVLLLKSALTLHFFKRDFEVLFVHKYSGKMVLDSASI 120
Query: 137 ISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGNMYHHILLSQTRKKGET 196
ISLSYFSSTALMIYAQQ SKALPEPTIDLKNIGIA+F+IGIIGNMYHHILLSQTRKKGET
Sbjct: 121 ISLSYFSSTALMIYAQQLSKALPEPTIDLKNIGIALFLIGIIGNMYHHILLSQTRKKGET 180
Query: 197 GYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIATAIYLGGRSYATRKWYV 256
GYKIPKGGLFGI+ICPHY+FEITVYFGFALISQTFY+FFFAIATAIYLGGRSYAT KWYV
Sbjct: 181 GYKIPKGGLFGIVICPHYLFEITVYFGFALISQTFYSFFFAIATAIYLGGRSYATWKWYV 240
Query: 257 SKFEDFPQHIKALIPFVF 275
SKFEDFPQHIKALIPFVF
Sbjct: 241 SKFEDFPQHIKALIPFVF 258
BLAST of CsaV3_5G035110 vs. NCBI nr
Match:
XP_038892749.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida])
HSP 1 Score: 464.9 bits (1195), Expect = 4.7e-127
Identity = 236/268 (88.06%), Postives = 249/268 (92.91%), Query Frame = 0
Query: 7 MNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSATKSSNSE 66
M+ LSAF FP PPSL VNVMTVVSS VVAVIG SEARGTHLQYSKFWNT+NSATKSSNSE
Sbjct: 1 MDILSAFLFPSPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTNNSATKSSNSE 60
Query: 67 QLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVLFVHKYS 126
QLRISSRLGMLLLYTPA LAGAASFWF P+DD R++LLKSALT HFFKRDFEVLFVHKYS
Sbjct: 61 QLRISSRLGMLLLYTPAALAGAASFWFFPDDDWRIVLLKSALTLHFFKRDFEVLFVHKYS 120
Query: 127 GKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGNMYHHIL 186
G M++DSA+IISLSYFSSTALMIY QQ SKAL EP+IDLKNIGIAVFVIGIIGNMYHH +
Sbjct: 121 GYMILDSAVIISLSYFSSTALMIYTQQLSKALVEPSIDLKNIGIAVFVIGIIGNMYHHYI 180
Query: 187 LSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIATAIYLGG 246
LSQTRKKGETGYKIPKGGLF I+ICPHY+FEITVYFGFALISQT Y+ FAIATAIYLGG
Sbjct: 181 LSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALISQTLYSLCFAIATAIYLGG 240
Query: 247 RSYATRKWYVSKFEDFPQHIKALIPFVF 275
RSYATRKWYVSKFEDFPQHIKALIPFVF
Sbjct: 241 RSYATRKWYVSKFEDFPQHIKALIPFVF 268
BLAST of CsaV3_5G035110 vs. NCBI nr
Match:
XP_023518338.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 442.6 bits (1137), Expect = 2.5e-120
Identity = 223/271 (82.29%), Postives = 246/271 (90.77%), Query Frame = 0
Query: 5 AAMNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGT-HLQYSKFWNTSNSATKSS 64
AAM+T+SA F LPPSLLVNVMTVVSSVVVAVIG SEARGT HLQYSKFWN +NSATKSS
Sbjct: 2 AAMDTISALLFRLPPSLLVNVMTVVSSVVVAVIGLSEARGTAHLQYSKFWNANNSATKSS 61
Query: 65 NSEQLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVLFVH 124
N E+ +ISSR GMLLLY PA LAGA SFW PNDDLR+LLLKSALT HFFKR+FEVLF+H
Sbjct: 62 NPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNDDLRILLLKSALTLHFFKRNFEVLFIH 121
Query: 125 KYSGKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGNMYH 184
KYSGKM +DSALIISLSYFSSTALMIYAQ+ S+ LPEP+IDLK IGI++F+IGIIGN YH
Sbjct: 122 KYSGKMALDSALIISLSYFSSTALMIYAQELSRTLPEPSIDLKFIGISLFLIGIIGNFYH 181
Query: 185 HILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIATAIY 244
H +LSQTRKKGETGYKIPKGGLF ++ICPHY+FEITVYFGFALISQT Y+ FAIATA+Y
Sbjct: 182 HYILSQTRKKGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMY 241
Query: 245 LGGRSYATRKWYVSKFEDFPQHIKALIPFVF 275
LGGRSYATRKWY+SKFEDFPQHIKAL+PFVF
Sbjct: 242 LGGRSYATRKWYLSKFEDFPQHIKALVPFVF 272
BLAST of CsaV3_5G035110 vs. ExPASy Swiss-Prot
Match:
Q2QDF6 (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)
HSP 1 Score: 92.0 bits (227), Expect = 1.1e-17
Identity = 64/219 (29.22%), Postives = 116/219 (52.97%), Query Frame = 0
Query: 70 ISSRLGMLLLYTPAVLAGAASFWFLPND----DLRVLLLKSALTFHFFKRDFEVLFVHKY 129
IS L L+ +P + +F+ P+ + + LL S FH+F R VL+ +
Sbjct: 45 ISPPLAWFLMESPTLW---LTFFLFPSGQHFYNPKSFLLISPFLFHYFNR--TVLYPLRL 104
Query: 130 SGKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKN---------IGIAVFVIG 189
+ +S+++ + ++ Q++ + D +N G+ +FV+G
Sbjct: 105 ARNTTQTRGFPVSVAFMAFGFNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVG 164
Query: 190 IIGNMYHHILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFF 249
+ N+ +L +K+G+ GYKIP+GGLF ++ CP+Y EI +FG+A+++ ++ F F
Sbjct: 165 MWVNVRADKVLVGLKKQGDGGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGF 224
Query: 250 AIATAIYLGGRSYATRKWYVSKF-EDFPQHIKALIPFVF 275
+ T L R+ ATR WY+ KF +D+P+ KA+IPF++
Sbjct: 225 FLYTCANLMPRARATRLWYLEKFKDDYPKDRKAVIPFIY 258
BLAST of CsaV3_5G035110 vs. ExPASy Swiss-Prot
Match:
Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)
HSP 1 Score: 88.6 bits (218), Expect = 1.2e-16
Identity = 43/107 (40.19%), Postives = 66/107 (61.68%), Query Frame = 0
Query: 168 IGIAVFVIGIIGNMYHHILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALI 227
IG +++ G++ N++ +L RK G+TGYKIP+GGLF + +Y EI + G+AL
Sbjct: 157 IGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 216
Query: 228 SQTFYAFFFAIATAIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF 275
S + FA T +L GR+ +WY+ KFE++P+ K LIPF+F
Sbjct: 217 SWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263
BLAST of CsaV3_5G035110 vs. ExPASy Swiss-Prot
Match:
P18405 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)
HSP 1 Score: 87.0 bits (214), Expect = 3.5e-16
Identity = 42/107 (39.25%), Postives = 66/107 (61.68%), Query Frame = 0
Query: 168 IGIAVFVIGIIGNMYHHILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALI 227
IG +++ G++ N++ +L RK G+TGYKIP+GGLF + +Y EI + G+AL
Sbjct: 153 IGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 212
Query: 228 SQTFYAFFFAIATAIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF 275
S + FA T +L GR+ +WY+ KFE++P+ K +IPF+F
Sbjct: 213 SWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259
BLAST of CsaV3_5G035110 vs. ExPASy Swiss-Prot
Match:
P24008 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 PE=1 SV=2)
HSP 1 Score: 86.7 bits (213), Expect = 4.6e-16
Identity = 44/105 (41.90%), Postives = 64/105 (60.95%), Query Frame = 0
Query: 169 GIAVFVIGIIGNMYHHILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALIS 228
G A++++G++ N++ +L RK GETGYKIP+GGLF + +Y E+ + GFAL S
Sbjct: 154 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSAANYFGELVEWCGFALAS 213
Query: 229 QTFYAFFFAIATAIYLGGRSYATRKWYVSKFEDFPQHIKALIPFV 274
+ FA+ T L R+ +WY KFED+P+ K LIPFV
Sbjct: 214 WSLQGVVFALFTLSTLLTRAKQHHQWYHEKFEDYPKSRKILIPFV 258
BLAST of CsaV3_5G035110 vs. ExPASy Swiss-Prot
Match:
Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)
HSP 1 Score: 85.9 bits (211), Expect = 7.8e-16
Identity = 59/192 (30.73%), Postives = 96/192 (50.00%), Query Frame = 0
Query: 91 FWFLPN-----DDLRVLLLKSAL---TFHFFKRDFEVLFVHKYSGKMVIDSALIISLSYF 150
F+FL N D + K AL + H+ KR +E +FVH++S + L + SY+
Sbjct: 108 FYFLSNLIYGTDSPKSFAQKVALVCYSLHYIKRIYETIFVHRFSHGTMPIFNLFKNCSYY 167
Query: 151 SSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGNMYHHILLSQTRKKGETGYKIPK 210
M+ E I+ +G+ +++IG + N HI L R G T KIP+
Sbjct: 168 WGCTAMVSYFVNHPLYTEAPIERVYLGLGLWIIGEVFNYICHIQLRNLRPAGSTERKIPR 227
Query: 211 GGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIATAIYLGGRSYATRKWYVSKFED- 270
G LF + CP+Y EI + GF++++QT ++ FA+ A + + + Y +F D
Sbjct: 228 GLLFEFVSCPNYTVEILSWIGFSILTQTLTSWIFALMGAAQMWIWAVGKHRRYRKEFGDK 287
Query: 271 FPQHIKALIPFV 274
+P+ K LIPF+
Sbjct: 288 YPKSRKILIPFL 299
BLAST of CsaV3_5G035110 vs. ExPASy TrEMBL
Match:
A0A0A0KRA7 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G612890 PE=3 SV=1)
HSP 1 Score: 532.3 bits (1370), Expect = 1.2e-147
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0
Query: 1 MQFTAAMNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSAT 60
MQFTAAMNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSAT
Sbjct: 1 MQFTAAMNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSAT 60
Query: 61 KSSNSEQLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVL 120
KSSNSEQLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVL
Sbjct: 61 KSSNSEQLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVL 120
Query: 121 FVHKYSGKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGN 180
FVHKYSGKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGN
Sbjct: 121 FVHKYSGKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGN 180
Query: 181 MYHHILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIAT 240
MYHHILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIAT
Sbjct: 181 MYHHILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIAT 240
Query: 241 AIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF 275
AIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF
Sbjct: 241 AIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF 274
BLAST of CsaV3_5G035110 vs. ExPASy TrEMBL
Match:
A0A1S3BGE8 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo OX=3656 GN=LOC103489361 PE=3 SV=1)
HSP 1 Score: 491.5 bits (1264), Expect = 2.3e-135
Identity = 250/268 (93.28%), Postives = 257/268 (95.90%), Query Frame = 0
Query: 7 MNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSATKSSNSE 66
MN LS FFFP+PPSLLVNVMTVVSS VVAVIG SEARGTHLQYSKFWNTSNSATKSSNSE
Sbjct: 1 MNALSVFFFPMPPSLLVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTSNSATKSSNSE 60
Query: 67 QLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVLFVHKYS 126
QLRISSRLGMLLLYTPA LAGAASFWF PNDD RVLLLKSALT HFFKRDFEVLFVHKYS
Sbjct: 61 QLRISSRLGMLLLYTPAALAGAASFWFFPNDDWRVLLLKSALTLHFFKRDFEVLFVHKYS 120
Query: 127 GKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGNMYHHIL 186
GKMV+DSA IISLSYFSSTALMIYAQQ SKALPEPTIDLKNIGIA+F+IGIIGNMYHHIL
Sbjct: 121 GKMVLDSASIISLSYFSSTALMIYAQQLSKALPEPTIDLKNIGIALFLIGIIGNMYHHIL 180
Query: 187 LSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIATAIYLGG 246
LSQTRKKGETGYKIPKGGLFGI+ICPHY+FEITVYFGFALISQTFY+FFFAIATAIYLGG
Sbjct: 181 LSQTRKKGETGYKIPKGGLFGIVICPHYLFEITVYFGFALISQTFYSFFFAIATAIYLGG 240
Query: 247 RSYATRKWYVSKFEDFPQHIKALIPFVF 275
RSYAT KWYVSKFEDFPQHIKALIPFVF
Sbjct: 241 RSYATWKWYVSKFEDFPQHIKALIPFVF 268
BLAST of CsaV3_5G035110 vs. ExPASy TrEMBL
Match:
A0A5D3CBG2 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001750 PE=3 SV=1)
HSP 1 Score: 475.7 bits (1223), Expect = 1.3e-130
Identity = 242/258 (93.80%), Postives = 249/258 (96.51%), Query Frame = 0
Query: 17 LPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSATKSSNSEQLRISSRLGM 76
+PPSLLVNVMTVVSS VVAVIG SEARGTHLQYSKFWNTSNSATKSSNSEQLRISSRLGM
Sbjct: 1 MPPSLLVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTSNSATKSSNSEQLRISSRLGM 60
Query: 77 LLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVLFVHKYSGKMVIDSALI 136
LLLYTPA LAGAASFWF PNDD RVLLLKSALT HFFKRDFEVLFVHKYSGKMV+DSA I
Sbjct: 61 LLLYTPAALAGAASFWFFPNDDWRVLLLKSALTLHFFKRDFEVLFVHKYSGKMVLDSASI 120
Query: 137 ISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGNMYHHILLSQTRKKGET 196
ISLSYFSSTALMIYAQQ SKALPEPTIDLKNIGIA+F+IGIIGNMYHHILLSQTRKKGET
Sbjct: 121 ISLSYFSSTALMIYAQQLSKALPEPTIDLKNIGIALFLIGIIGNMYHHILLSQTRKKGET 180
Query: 197 GYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIATAIYLGGRSYATRKWYV 256
GYKIPKGGLFGI+ICPHY+FEITVYFGFALISQTFY+FFFAIATAIYLGGRSYAT KWYV
Sbjct: 181 GYKIPKGGLFGIVICPHYLFEITVYFGFALISQTFYSFFFAIATAIYLGGRSYATWKWYV 240
Query: 257 SKFEDFPQHIKALIPFVF 275
SKFEDFPQHIKALIPFVF
Sbjct: 241 SKFEDFPQHIKALIPFVF 258
BLAST of CsaV3_5G035110 vs. ExPASy TrEMBL
Match:
A0A6J1H074 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458413 PE=3 SV=1)
HSP 1 Score: 439.1 bits (1128), Expect = 1.3e-119
Identity = 223/271 (82.29%), Postives = 244/271 (90.04%), Query Frame = 0
Query: 5 AAMNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGT-HLQYSKFWNTSNSATKSS 64
AAM+T+SA F PPSLLVNVMTVVSSVVVAVIG SEARGT HLQYSKFWNT+NSATKSS
Sbjct: 2 AAMDTISALLFRPPPSLLVNVMTVVSSVVVAVIGLSEARGTAHLQYSKFWNTNNSATKSS 61
Query: 65 NSEQLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVLFVH 124
N E+ +ISSR GMLLLY PA LAGA SFW PNDDLR+LLLKSALT HF KR+FEVLF+H
Sbjct: 62 NLEKFKISSRSGMLLLYAPAALAGAVSFWIFPNDDLRILLLKSALTLHFVKRNFEVLFIH 121
Query: 125 KYSGKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGNMYH 184
KYSGKM +DSALIISLSYFSSTALMIYAQ+ S+ LPEP+IDLK IGIA+F+IGIIGN YH
Sbjct: 122 KYSGKMALDSALIISLSYFSSTALMIYAQELSRTLPEPSIDLKFIGIALFLIGIIGNFYH 181
Query: 185 HILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIATAIY 244
H +LSQTRKKGETGYKIPKGGLF ++ICPHY+FEITVYFGFALISQT Y+ FAIATA+Y
Sbjct: 182 HYILSQTRKKGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMY 241
Query: 245 LGGRSYATRKWYVSKFEDFPQHIKALIPFVF 275
LGGRSYATRKWYVSKFEDFP HIKAL+PFVF
Sbjct: 242 LGGRSYATRKWYVSKFEDFPHHIKALVPFVF 272
BLAST of CsaV3_5G035110 vs. ExPASy TrEMBL
Match:
A0A6J1J2Y2 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482885 PE=3 SV=1)
HSP 1 Score: 431.8 bits (1109), Expect = 2.2e-117
Identity = 218/271 (80.44%), Postives = 243/271 (89.67%), Query Frame = 0
Query: 5 AAMNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGT-HLQYSKFWNTSNSATKSS 64
AAM+T+S F PPSLLVNVMTVVSSVVVAVIG SEA+GT HLQYSKFWNT+NSATKSS
Sbjct: 2 AAMDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKSS 61
Query: 65 NSEQLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVLFVH 124
+ E+ +ISSR GMLLLY PA LAGA SFW PN+DLR+LLLKSALT HF KR+FEVLF+H
Sbjct: 62 SPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFEVLFIH 121
Query: 125 KYSGKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGNMYH 184
KYSGKM +DSALIISLSYFSSTALMI+AQ+ S+ LPEP+IDLK IGIA+F+IGIIGN YH
Sbjct: 122 KYSGKMALDSALIISLSYFSSTALMIFAQELSRTLPEPSIDLKFIGIALFLIGIIGNFYH 181
Query: 185 HILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIATAIY 244
H +LSQTRKKGETGYKIPKGGLF ++ICPHY+FEITVYFGFALISQT Y+ FAIATA+Y
Sbjct: 182 HYILSQTRKKGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMY 241
Query: 245 LGGRSYATRKWYVSKFEDFPQHIKALIPFVF 275
LGGRSYATRKWYVSKFEDFP HIKAL+PFVF
Sbjct: 242 LGGRSYATRKWYVSKFEDFPHHIKALVPFVF 272
BLAST of CsaV3_5G035110 vs. TAIR 10
Match:
AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )
HSP 1 Score: 325.1 bits (832), Expect = 5.5e-89
Identity = 168/269 (62.45%), Postives = 210/269 (78.07%), Query Frame = 0
Query: 7 MNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSATKSSNSE 66
M +++F +P PPS+L+N M+VV +A IG+SE RG HL+YSKF S+S+ +
Sbjct: 1 MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKF-GVSSSSPQPQKER 60
Query: 67 QLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVLFVHKYS 126
ISSR GMLLLYTPA LA A+SF+ +P+DDLR LLLKSAL HFFKR FEVLF+HKYS
Sbjct: 61 FGSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYS 120
Query: 127 GKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGNMYHHIL 186
G M IDSAL IS SYFSSTALM+Y+Q + L EP+ D+K G+ +FV+GI+GN+YHH+L
Sbjct: 121 GGMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVL 180
Query: 187 LSQTRKK-GETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIATAIYLG 246
L++ RK+ G+ YKIPKGGLF IIICPHY+FEI V++ F LISQT Y+F FA+ T +YL
Sbjct: 181 LAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLI 240
Query: 247 GRSYATRKWYVSKFEDFPQHIKALIPFVF 275
GRSYATR WY+SKF+DFP+HIKALIPFVF
Sbjct: 241 GRSYATRTWYLSKFDDFPKHIKALIPFVF 268
BLAST of CsaV3_5G035110 vs. TAIR 10
Match:
AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )
HSP 1 Score: 76.3 bits (186), Expect = 4.4e-14
Identity = 39/108 (36.11%), Postives = 67/108 (62.04%), Query Frame = 0
Query: 168 IGIAVFVIGIIGNMYHHILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALI 227
IG+ VF+ G+ N+ L + +K+ GY IP+GG F ++ CP+Y E + G+A++
Sbjct: 155 IGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVM 214
Query: 228 SQTFYAFFFAIATAIYLGGRSYATRKWYVSKF-EDFPQHIKALIPFVF 275
+ ++ F + T L R+ A+ KWY++KF E++P+ KA+IPFV+
Sbjct: 215 TWSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY 262
BLAST of CsaV3_5G035110 vs. TAIR 10
Match:
AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )
HSP 1 Score: 67.4 bits (163), Expect = 2.1e-11
Identity = 46/137 (33.58%), Postives = 68/137 (49.64%), Query Frame = 0
Query: 110 FHFFKRDFEVLFVHKYSGKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLK-NI 169
FH+FKR E FVH++S + + +Y+ S I A + L P DL+ I
Sbjct: 140 FHYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYI-AYYVNHPLYTPVSDLQMKI 199
Query: 170 GIAVFVIGIIGNMYHHILLSQTR-KKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALI 229
G ++ + N Y HILL R G GY+IP+G LF I+ C +Y EI + GF +
Sbjct: 200 GFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIA 259
Query: 230 SQTFYAFFFAIATAIYL 245
+QT + F A+ +
Sbjct: 260 TQTIAGYVFLAVAALIM 275
BLAST of CsaV3_5G035110 vs. TAIR 10
Match:
AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )
HSP 1 Score: 60.8 bits (146), Expect = 1.9e-09
Identity = 38/117 (32.48%), Postives = 58/117 (49.57%), Query Frame = 0
Query: 165 LKNIGIAVFVIGIIGNMYHHILLSQTRKKGETG--YKIPKGGLFGIIICPHYMFEITVYF 224
L+ IG A+F+ G I H +L R+ Y IP G FG++ PH++ EI +Y
Sbjct: 230 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 289
Query: 225 GFALIS-----QTFYAFFFAIATAIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF 275
G + S + F F A Y G T +WY+ KFE++P + A+ P+V+
Sbjct: 290 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 343
BLAST of CsaV3_5G035110 vs. TAIR 10
Match:
AT2G16530.2 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )
HSP 1 Score: 60.8 bits (146), Expect = 1.9e-09
Identity = 38/117 (32.48%), Postives = 58/117 (49.57%), Query Frame = 0
Query: 165 LKNIGIAVFVIGIIGNMYHHILLSQTRKKGETG--YKIPKGGLFGIIICPHYMFEITVYF 224
L+ IG A+F+ G I H +L R+ Y IP G FG++ PH++ EI +Y
Sbjct: 229 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 288
Query: 225 GFALIS-----QTFYAFFFAIATAIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF 275
G + S + F F A Y G T +WY+ KFE++P + A+ P+V+
Sbjct: 289 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 342
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_011655823.1 | 2.4e-147 | 100.00 | steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN52160.1 hypothetical protei... | [more] |
XP_008446723.1 | 4.7e-135 | 93.28 | PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo] | [more] |
KAA0034632.1 | 2.7e-130 | 93.80 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo var. makuwa] >TYK0918... | [more] |
XP_038892749.1 | 4.7e-127 | 88.06 | 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida] | [more] |
XP_023518338.1 | 2.5e-120 | 82.29 | 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
Q2QDF6 | 1.1e-17 | 29.22 | Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1 | [more] |
Q28891 | 1.2e-16 | 40.19 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1... | [more] |
P18405 | 3.5e-16 | 39.25 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 S... | [more] |
P24008 | 4.6e-16 | 41.90 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 ... | [more] |
Q55C17 | 7.8e-16 | 30.73 | Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn... | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0KRA7 | 1.2e-147 | 100.00 | S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6128... | [more] |
A0A1S3BGE8 | 2.3e-135 | 93.28 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo OX=3656 GN=LOC10348... | [more] |
A0A5D3CBG2 | 1.3e-130 | 93.80 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo var. makuwa OX=1194... | [more] |
A0A6J1H074 | 1.3e-119 | 82.29 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO... | [more] |
A0A6J1J2Y2 | 2.2e-117 | 80.44 | 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC1... | [more] |
Match Name | E-value | Identity | Description | |
AT5G16010.1 | 5.5e-89 | 62.45 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |
AT2G38050.1 | 4.4e-14 | 36.11 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |
AT3G55360.1 | 2.1e-11 | 33.58 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |
AT2G16530.1 | 1.9e-09 | 32.48 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |
AT2G16530.2 | 1.9e-09 | 32.48 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |