CsaV3_5G032610 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_5G032610
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Descriptionsphinganine C4-monooxygenase 2
Locationchr5: 26379142 .. 26382683 (+)
RNA-Seq ExpressionCsaV3_5G032610
SyntenyCsaV3_5G032610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAGGTTGCCACGTGTATGACGAGCTCCGAATCAAACCTTTTCTTCTCCACGATGACATTTTCATCGTTGGAAATGGAATAATTATTTTTTCTTTTAAAAAAAAAACGACGTAGTTTGATTCTTCTCGATCTCCATCCTCTCTCTTCCATGATTCGTACCACTCCCACAGATTTTTCTTTGTTTTTTCTGTAAACCCTAGCTCGAATCCTTTTTCATTTTCCTCTTCTTCCGTTGATTTTGATTTCTGGGTGTTCATTTGTTCCGTTTCTTTTCCTTCTTGTAGAAAGGCTAGTGTAGGAAGGTGATCCCCTGTTCTCCTTTCCCATCATAGGAATTGGATTTGGGATTCCCTTCCTGCTCTTTCTTAACCTGCCGGAAAACTGTACGTGAGCTGAGATTTGGGTTTTTGGTTCCTAATGCAATTCCGGCTGCTTTTGGGGTTTAGTATTTGATCTTTTCTAGTTCCTTTTCCAACATGGGTATTGTAATCTCTGATCAAATTTTGGGTACTTTTGTACCAATTGTGGTTTATTGGCTATATTCTGGGCTTTACATTCTTCTTGGCTCATTTGAGAACTACCGGTTACACTCTAAGAAAGATGAGCTGGAGAAGAACTTAGTGTCTAAAAGCACTGTGGTTAGAGGCGTTTTCCTTCAACAGATTATTCAGGCAATCGTTGCAATCCTCTTGTTTAAGGTATGCATTGAATAATCTCCACTAAAGTTCATTAATTTCTATGAGTGTATGCTTTTCATCATACTTTGTGACTTTGTCAACCTTTGTATGAAGTCGGGATCAAATATCAATAGGGTATTAATAGTTGAAAGAGGTGGCAATGAGTTATATGGAAAGTGTAAGAATCACTTGATGTGAATTTTATCTCTGCCCGGTCCCCCTCTCGTGGGTTTGTGGACACTAGATATAGCATTAAGTCTGGACTGTTTGTTGAGAAGTAATTGTACTAGTGATTACTGATCAGTACTGCGAAATTTATTACTGGTTGACTTGCTTGGTTCTTGTTAAATTTTATGAACTATATATTACCAGCATCAGTTGTGGAGTGTTGGACCTTTGTACTTCCTAGTCTTCCAGACCAAGATTGATCAATATATTTTTTTTCTGAAGATTCATTTTGTTTGCGCTGAATCACTTATTTCTTCTGGTTAGGTCACTTTCCTTGAAAAACTTTCATCCGTGATTCTTGAACTACTCTGCTTCAAAATTGTTGTCTCCCCTGCAAAATAAAGAGTTAATAAAGGAGAGAAAAGTAGTATCCCAAAATGTCACTGTATTTTAGTTTTGATTAGTTAGTCGTGTGTATAAACATCTTCTATTGAAAGAACATGTTTTTCCACTGGTTTTTTATGAAACTTTTGTTTGCTTTTTCTTTGGTGTGTGCATCTCAAATGAAAATTAATCCTTGAGTTGTCTCATGGATGCCACCAGATTTACTCTACTGCAACTAGTGGTGAATGGTGCATAAGTCTCAATCACTTTCCCCCCATATGTCTTGGGATTTAGGGGTTAAGGAAAAGTAAGGGACACAATTAATCTAGGTTGATAGGCTGCGCACCTTTTTTTTTATTCCTGGTCTACATCTGAGATTACTCAGCTGTGCTTCAATTGTAACTTTCTACATTGATAGCATCTTTTTTTGGGTTCTGCTGTCCTTAATATGAGTTTCAATAGTGAAGCATATTGGGCCACATTTCTTATATACACGTCTCTGCAGTCTTTTTCAGTGTCTGATTTCCTTCACATATATTTGTTTACTCACCATTCGAGTACTGGCTGTAAATGTCAACTAGCCATTATTCAATATGATTAAGAAACAAAAATTGGTTTGTTCTGTTGAAGTCAGTTTTTGTGATGTGGAAAACCTGCACATTCGTGAAAATGAAGGGATTAAAGAACTTATTTTCAAGGGGCCAAAATTGCAATGTTATATTGGTTAAGCATTATTCTGTAGATCCTGCAATTAATTTCTGATATGCTGGATCTAATCATTTCTCTTTTCATGCTGAACATTACGTGTTTCATTAGTTTATCAGTTTGTCCTGCATCTAATGCAATGGAGGTGTAATTAATTCACTAAAACTTAGATTCATAACATGGCTAATGATTGGGCAGCTCTCATTTGTTACGTTATATTTTCTATGGCTGAGTGGATTTGACATGGGAAAGTTGATTTGATCATTGCCTGTATGCTGACAACTGCTTTTGTTCTGAATATGTAGGTAACTGAAACTGATGGGGAGGTTGCTACAGGTCCAAAATCTTGGCTAACAATTGTAGTTCAGTTTATTGTTGCTATGCTAGTGTTGGACACTTGGCAATATTTCATTCACAGATACATGCATCAGAACAAGTTTCTGTACAAGCACGTTCATTCCCAGCATCACCGTTTGGTTGTACCTTATGCATTTGGAGCTTTATACAATCATCCGTTGGAGGGTCTTCTCCTTGACACAATTGGTGGGGCTTTATCTTTTCTCGTTTCTGGGATGTCACCTCGAGTTGCCATCTTTTTCTTTTCCTTTGCCACCATCAAGACAGTGGATGACCATTGTGGATTATGGCTTCCTGGGAACCTTTTCCACGTGTTCTTCAGAAACAATTCTGCATATCATGATGTTCATCACCAGCTATATGGCAGTAAGTATAACTTCTCACAGCCATTTTTTGTTACGTGGGATAGAATATTTGGCACCTATATGCCTTACTCATTAGAGAAGAGAGCAGGGGGTGGTTTCGAAGCACGAGTTAAGGAAGATTAGTTCAAATAGCAATCATATATTATTTGCGTCCGTGTTAATTTTTTCTCTCCCTGTTAGCCAACCACCATAGTGCTAAATTTGTATCATACACCTATTCTTGTGTCTTACACTTTTGTTACTATCATTTCCTCGAAGTTCCATAGCTGAATCATGTTGTGATTTGAACCATTTTCCTTTTTTTTTTTTTTTCTTTTCTTGTACCTAATCCTTTTGAACAGATAACCCCCTTGACTTTTTTCCTTTTTCAAATTGGGGTAACCGGTACTTAATGACATTGTGAAGTATATCTTTCTTGTGCTAATTTAGATTGATCCGATCTGGTAAAGAAGCAAATGTAGGCCAAACATTGTTATGTGGCTTGGGGGATGAGCGATTTTCATCTAAATTTTGACACGTAAGGAGAGAATGTGCATGTGGATAGGTTAGGCTCATTTTATTTAGTTTCACATGGTATGATTAATAGATATCATTTCTTAAATAAAGATACAAGTGAAATGATATAACTTTATGAATTGAACAATAAGCATATATTAATGCAGTATATTTTAGGACCCAGTTGCTTCATTGAAACTAAGCAAGCTTGGCAACAACTGTCGGTCAAACATCAGATCACATTAGACAGCTTGACTTTAGCTATTGCCAGTTAATTGCAGGAATATAATACTTTGGTGTCTTATGTCTCTATATGATCTGGAATTTGCAATGCCCATCTATATATATATATTGGGAAATGTAACTTCCTTGTTTCTGACAAAAGTTTTTC

mRNA sequence

ATGGGTATTGTAATCTCTGATCAAATTTTGGGTACTTTTGTACCAATTGTGGTTTATTGGCTATATTCTGGGCTTTACATTCTTCTTGGCTCATTTGAGAACTACCGGTTACACTCTAAGAAAGATGAGCTGGAGAAGAACTTAGTGTCTAAAAGCACTGTGGTTAGAGGCGTTTTCCTTCAACAGATTATTCAGGCAATCGTTGCAATCCTCTTGTTTAAGGTAACTGAAACTGATGGGGAGGTTGCTACAGGTCCAAAATCTTGGCTAACAATTGTAGTTCAGTTTATTGTTGCTATGCTAGTGTTGGACACTTGGCAATATTTCATTCACAGATACATGCATCAGAACAAGTTTCTGTACAAGCACGTTCATTCCCAGCATCACCGTTTGGTTGTACCTTATGCATTTGGAGCTTTATACAATCATCCGTTGGAGGGTCTTCTCCTTGACACAATTGGTGGGGCTTTATCTTTTCTCGTTTCTGGGATGTCACCTCGAGTTGCCATCTTTTTCTTTTCCTTTGCCACCATCAAGACAGTGGATGACCATTGTGGATTATGGCTTCCTGGGAACCTTTTCCACGTGTTCTTCAGAAACAATTCTGCATATCATGATGTTCATCACCAGCTATATGGCAGTAAGTATAACTTCTCACAGCCATTTTTTGTTACGTGGGATAGAATATTTGGCACCTATATGCCTTACTCATTAGAGAAGAGAGCAGGGGGTGGTTTCGAAGCACGAGTTAAGGAAGATTAG

Coding sequence (CDS)

ATGGGTATTGTAATCTCTGATCAAATTTTGGGTACTTTTGTACCAATTGTGGTTTATTGGCTATATTCTGGGCTTTACATTCTTCTTGGCTCATTTGAGAACTACCGGTTACACTCTAAGAAAGATGAGCTGGAGAAGAACTTAGTGTCTAAAAGCACTGTGGTTAGAGGCGTTTTCCTTCAACAGATTATTCAGGCAATCGTTGCAATCCTCTTGTTTAAGGTAACTGAAACTGATGGGGAGGTTGCTACAGGTCCAAAATCTTGGCTAACAATTGTAGTTCAGTTTATTGTTGCTATGCTAGTGTTGGACACTTGGCAATATTTCATTCACAGATACATGCATCAGAACAAGTTTCTGTACAAGCACGTTCATTCCCAGCATCACCGTTTGGTTGTACCTTATGCATTTGGAGCTTTATACAATCATCCGTTGGAGGGTCTTCTCCTTGACACAATTGGTGGGGCTTTATCTTTTCTCGTTTCTGGGATGTCACCTCGAGTTGCCATCTTTTTCTTTTCCTTTGCCACCATCAAGACAGTGGATGACCATTGTGGATTATGGCTTCCTGGGAACCTTTTCCACGTGTTCTTCAGAAACAATTCTGCATATCATGATGTTCATCACCAGCTATATGGCAGTAAGTATAACTTCTCACAGCCATTTTTTGTTACGTGGGATAGAATATTTGGCACCTATATGCCTTACTCATTAGAGAAGAGAGCAGGGGGTGGTTTCGAAGCACGAGTTAAGGAAGATTAG

Protein sequence

MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFLQQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFLYKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKTVDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEKRAGGGFEARVKED*
Homology
BLAST of CsaV3_5G032610 vs. NCBI nr
Match: XP_004135460.1 (sphinganine C4-monooxygenase 2 [Cucumis sativus] >KGN51908.1 hypothetical protein Csa_008088 [Cucumis sativus])

HSP 1 Score: 513.1 bits (1320), Expect = 1.4e-141
Identity = 253/253 (100.00%), Postives = 253/253 (100.00%), Query Frame = 0

Query: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60
           MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL
Sbjct: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60

Query: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180
           YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT
Sbjct: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240
           VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240

Query: 241 RAGGGFEARVKED 254
           RAGGGFEARVKED
Sbjct: 241 RAGGGFEARVKED 253

BLAST of CsaV3_5G032610 vs. NCBI nr
Match: XP_008446343.1 (PREDICTED: sphinganine C4-monooxygenase 2 [Cucumis melo] >KAA0034409.1 sphinganine C4-monooxygenase 2 [Cucumis melo var. makuwa] >TYK17723.1 sphinganine C4-monooxygenase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 511.5 bits (1316), Expect = 4.1e-141
Identity = 252/253 (99.60%), Postives = 252/253 (99.60%), Query Frame = 0

Query: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60
           MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL
Sbjct: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60

Query: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQIIQAIVAILLFKVTETDGEVATGPKSWLTI VQFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIAVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180
           YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT
Sbjct: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240
           VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240

Query: 241 RAGGGFEARVKED 254
           RAGGGFEARVKED
Sbjct: 241 RAGGGFEARVKED 253

BLAST of CsaV3_5G032610 vs. NCBI nr
Match: XP_022151262.1 (sphinganine C4-monooxygenase 2-like [Momordica charantia])

HSP 1 Score: 498.0 bits (1281), Expect = 4.7e-137
Identity = 243/253 (96.05%), Postives = 248/253 (98.02%), Query Frame = 0

Query: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60
           MGIVISDQ LGTFVPIVVYW+YSG+YILLGSFENYRLHSKKDELEKNLVSKSTVV GVFL
Sbjct: 1   MGIVISDQFLGTFVPIVVYWVYSGIYILLGSFENYRLHSKKDELEKNLVSKSTVVGGVFL 60

Query: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQIIQAIVAILLFKVT  DGEVA+GPK WLTI+VQFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQIIQAIVAILLFKVTGNDGEVASGPKPWLTIIVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180
           YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFL+SGMSPRVAIFFFSFATIKT
Sbjct: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLISGMSPRVAIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240
           VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240

Query: 241 RAGGGFEARVKED 254
           RAGGGFEARVKED
Sbjct: 241 RAGGGFEARVKED 253

BLAST of CsaV3_5G032610 vs. NCBI nr
Match: XP_022987574.1 (sphinganine C4-monooxygenase 2-like [Cucurbita maxima] >KAG7032210.1 Sphinganine C4-monooxygenase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 495.7 bits (1275), Expect = 2.3e-136
Identity = 241/253 (95.26%), Postives = 248/253 (98.02%), Query Frame = 0

Query: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60
           MGIVISDQILGTFVPI+VYWLYSG+Y+LLGSF+NYRLHSKKDE EKNLVSKSTV+RGVF 
Sbjct: 1   MGIVISDQILGTFVPILVYWLYSGIYVLLGSFDNYRLHSKKDEQEKNLVSKSTVLRGVFF 60

Query: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQ IQAIVAILLFKVTE +GEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQTIQAIVAILLFKVTENEGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180
           YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT
Sbjct: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240
           VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTY PYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYRPYSLEK 240

Query: 241 RAGGGFEARVKED 254
           RAGGGFEAR+KED
Sbjct: 241 RAGGGFEARLKED 253

BLAST of CsaV3_5G032610 vs. NCBI nr
Match: XP_038892349.1 (sphinganine C4-monooxygenase 2 [Benincasa hispida])

HSP 1 Score: 495.4 bits (1274), Expect = 3.0e-136
Identity = 243/253 (96.05%), Postives = 246/253 (97.23%), Query Frame = 0

Query: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60
           MG VISDQILGTFVPI+VYWLYSG YILLGSFENYRLHSKKDE EKNLVSKSTVVRGVFL
Sbjct: 1   MGSVISDQILGTFVPILVYWLYSGFYILLGSFENYRLHSKKDEQEKNLVSKSTVVRGVFL 60

Query: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQ IQA+VAILLFKVTE DGEVA G KSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQTIQAVVAILLFKVTENDGEVAAGSKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180
           YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRV+IFFFSFATIKT
Sbjct: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240
           VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240

Query: 241 RAGGGFEARVKED 254
           RAGGGFEARVKED
Sbjct: 241 RAGGGFEARVKED 253

BLAST of CsaV3_5G032610 vs. ExPASy Swiss-Prot
Match: Q9AST3 (Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=SBH2 PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 2.9e-113
Identity = 195/250 (78.00%), Postives = 220/250 (88.00%), Query Frame = 0

Query: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60
           M  VISD+ LGTFVPI+VYW+YSG+YI LGS + YRLHSK DE EKNLVSKS VV+GV L
Sbjct: 2   MSFVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLL 61

Query: 61  QQIIQAIVAILLFKVTETDGEVATGPK-SWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKF 120
           QQ +QAI++++LFK+T +D + AT  + S L +  QFI+AMLV+DTWQYFIHRYMH NKF
Sbjct: 62  QQTLQAIISVILFKITGSDADAATTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNKF 121

Query: 121 LYKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIK 180
           LYKH+HSQHHRL+VPY++GALYNHPLEGLLLDTIGGALSFL SGMSPR AIFFFSFATIK
Sbjct: 122 LYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFATIK 181

Query: 181 TVDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLE 240
           TVDDHCGLWLPGN FH+FF NNSAYHDVHHQLYG+KYNFSQPFFV WDRI GTY+PYSLE
Sbjct: 182 TVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWDRILGTYLPYSLE 241

Query: 241 KRAGGGFEAR 250
           KRA GGFE R
Sbjct: 242 KRANGGFETR 251

BLAST of CsaV3_5G032610 vs. ExPASy Swiss-Prot
Match: Q8VYI1 (Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=SBH1 PE=1 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 4.9e-113
Identity = 192/250 (76.80%), Postives = 221/250 (88.40%), Query Frame = 0

Query: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60
           MG  +SD++LGT  PIVVYWLYSG+Y+ L S E+YRLHSK +E EKNLVSKS+VV+GV +
Sbjct: 3   MGFAVSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLV 62

Query: 61  QQIIQAIVAILLFKVTETDGEVATGPK-SWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKF 120
           QQ++QA+VAILLF VT +D E     + S+L +  QF+ AM+VLDTWQYF+HRYMHQNKF
Sbjct: 63  QQVVQAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKF 122

Query: 121 LYKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIK 180
           LYKH+HSQHHRL+VPYA+GALYNHP+EGLLLDTIGGALSFLVSGMSPR +IFFFSFATIK
Sbjct: 123 LYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIK 182

Query: 181 TVDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLE 240
           TVDDHCGLWLPGNLFH+ F+NNSAYHD+HHQLYG+KYNFSQPFFV WDRI GTYMPYSLE
Sbjct: 183 TVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLE 242

Query: 241 KRAGGGFEAR 250
           KR  GGFEAR
Sbjct: 243 KREDGGFEAR 252

BLAST of CsaV3_5G032610 vs. ExPASy Swiss-Prot
Match: B8B4W4 (Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. indica OX=39946 GN=GL1-9 PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 2.3e-94
Identity = 164/252 (65.08%), Postives = 200/252 (79.37%), Query Frame = 0

Query: 5   ISDQILGTFVPIVVYWLYSGLYILL---GSFENYRLHSKKDELEKNLVSKSTVVRGVFLQ 64
           +SD+ +GTF PI +YW+Y+G Y L+      E YRLH++ +E EKNLV+   VVRGV LQ
Sbjct: 8   VSDETMGTFAPIALYWVYAGGYQLVLHRRPLERYRLHTRAEEEEKNLVALPAVVRGVLLQ 67

Query: 65  QIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFLY 124
           Q++QAIVA++LF VT     V   P   +    QF+VAMLV+D+WQYF+HRYMHQNKFLY
Sbjct: 68  QLVQAIVAMILFMVTSDSSAVVVQPPI-IIQAFQFLVAMLVMDSWQYFVHRYMHQNKFLY 127

Query: 125 KHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKTV 184
           +H+HSQHHRL+VPYA GALYNHPLEGLLLDT+GGA+SFLVSGM+PR ++FFF FA +KTV
Sbjct: 128 RHIHSQHHRLIVPYAIGALYNHPLEGLLLDTVGGAISFLVSGMTPRTSVFFFCFAVLKTV 187

Query: 185 DDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEKR 244
           DDHCGLWLP N+F   F+NN+AYHDVHHQL GSKYN+SQPFF  WDRI GT+MPY+L +R
Sbjct: 188 DDHCGLWLPYNIFQSLFQNNTAYHDVHHQLQGSKYNYSQPFFSIWDRILGTHMPYNLVRR 247

Query: 245 AGGGFEARVKED 254
             GGFEAR   D
Sbjct: 248 KEGGFEARPLRD 258

BLAST of CsaV3_5G032610 vs. ExPASy Swiss-Prot
Match: Q0D4G3 (Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-9 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 2.3e-94
Identity = 164/252 (65.08%), Postives = 200/252 (79.37%), Query Frame = 0

Query: 5   ISDQILGTFVPIVVYWLYSGLYILL---GSFENYRLHSKKDELEKNLVSKSTVVRGVFLQ 64
           +SD+ +GTF PI +YW+Y+G Y L+      E YRLH++ +E EKNLV+   VVRGV LQ
Sbjct: 8   VSDETMGTFAPIALYWVYAGGYQLVLHRRPLERYRLHTRAEEEEKNLVALPAVVRGVLLQ 67

Query: 65  QIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFLY 124
           Q++QAIVA++LF VT     V   P   +    QF+VAMLV+D+WQYF+HRYMHQNKFLY
Sbjct: 68  QLVQAIVAMILFMVTSDSSAVVVQPPI-IIQAFQFLVAMLVMDSWQYFVHRYMHQNKFLY 127

Query: 125 KHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKTV 184
           +H+HSQHHRL+VPYA GALYNHPLEGLLLDT+GGA+SFLVSGM+PR ++FFF FA +KTV
Sbjct: 128 RHIHSQHHRLIVPYAIGALYNHPLEGLLLDTVGGAISFLVSGMTPRTSVFFFCFAVLKTV 187

Query: 185 DDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEKR 244
           DDHCGLWLP N+F   F+NN+AYHDVHHQL GSKYN+SQPFF  WDRI GT+MPY+L +R
Sbjct: 188 DDHCGLWLPYNIFQSLFQNNTAYHDVHHQLQGSKYNYSQPFFSIWDRILGTHMPYNLVRR 247

Query: 245 AGGGFEARVKED 254
             GGFEAR   D
Sbjct: 248 KEGGFEARPLRD 258

BLAST of CsaV3_5G032610 vs. ExPASy Swiss-Prot
Match: O94298 (Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sur2 PE=3 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 2.5e-53
Identity = 109/253 (43.08%), Postives = 148/253 (58.50%), Query Frame = 0

Query: 15  PIVVYWLYSGL-----YILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFLQQIIQAIVA 74
           P+++YW+ S       YI L  FE YR+H  ++   +N V +  VV+ V  QQ+ + +V 
Sbjct: 21  PVIIYWVASAFFGFLHYIELPVFEKYRIHPPEEIARRNRVPQMAVVKAVLFQQLCEVVVG 80

Query: 75  ILL-----------------------FKVTETDGEVAT-GPKSWLTIVV---QFIVAMLV 134
           I L                        K      +VA   PK     +V   Q+  A  +
Sbjct: 81  IALAMFEGYPEPIDEAKQMLRYEAFFSKNLPALLQVAPFAPKLAYNFIVPAFQYFFAFFI 140

Query: 135 LDTWQYFIHRYMHQNKFLYKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVS 194
           +D+WQYF HRY+H NK LY  +H+ HHRL VPYA GALYNHP EGL+LDT G  +++L +
Sbjct: 141 IDSWQYFWHRYLHYNKKLYNMIHAHHHRLQVPYAMGALYNHPFEGLILDTFGAGVAYLAA 200

Query: 195 GMSPRVAIFFFSFATIKTVDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPF 236
           G+SP+ A+ FF+ +T+KTVDDHCG   P +   +FF NN+ YHD+HHQ YG + NFSQPF
Sbjct: 201 GLSPQQAVIFFTLSTLKTVDDHCGYVFPYDPLQMFFANNARYHDLHHQPYGFQKNFSQPF 260

BLAST of CsaV3_5G032610 vs. ExPASy TrEMBL
Match: A0A0A0KQL6 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G605020 PE=3 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 6.8e-142
Identity = 253/253 (100.00%), Postives = 253/253 (100.00%), Query Frame = 0

Query: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60
           MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL
Sbjct: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60

Query: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180
           YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT
Sbjct: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240
           VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240

Query: 241 RAGGGFEARVKED 254
           RAGGGFEARVKED
Sbjct: 241 RAGGGFEARVKED 253

BLAST of CsaV3_5G032610 vs. ExPASy TrEMBL
Match: A0A5A7SZ77 (Sphinganine C4-monooxygenase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1932G00520 PE=3 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 2.0e-141
Identity = 252/253 (99.60%), Postives = 252/253 (99.60%), Query Frame = 0

Query: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60
           MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL
Sbjct: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60

Query: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQIIQAIVAILLFKVTETDGEVATGPKSWLTI VQFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIAVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180
           YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT
Sbjct: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240
           VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240

Query: 241 RAGGGFEARVKED 254
           RAGGGFEARVKED
Sbjct: 241 RAGGGFEARVKED 253

BLAST of CsaV3_5G032610 vs. ExPASy TrEMBL
Match: A0A1S3BEC5 (sphinganine C4-monooxygenase 2 OS=Cucumis melo OX=3656 GN=LOC103489111 PE=3 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 2.0e-141
Identity = 252/253 (99.60%), Postives = 252/253 (99.60%), Query Frame = 0

Query: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60
           MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL
Sbjct: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60

Query: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQIIQAIVAILLFKVTETDGEVATGPKSWLTI VQFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIAVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180
           YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT
Sbjct: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240
           VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240

Query: 241 RAGGGFEARVKED 254
           RAGGGFEARVKED
Sbjct: 241 RAGGGFEARVKED 253

BLAST of CsaV3_5G032610 vs. ExPASy TrEMBL
Match: A0A6J1DD01 (sphinganine C4-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111019225 PE=3 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.3e-137
Identity = 243/253 (96.05%), Postives = 248/253 (98.02%), Query Frame = 0

Query: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60
           MGIVISDQ LGTFVPIVVYW+YSG+YILLGSFENYRLHSKKDELEKNLVSKSTVV GVFL
Sbjct: 1   MGIVISDQFLGTFVPIVVYWVYSGIYILLGSFENYRLHSKKDELEKNLVSKSTVVGGVFL 60

Query: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQIIQAIVAILLFKVT  DGEVA+GPK WLTI+VQFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQIIQAIVAILLFKVTGNDGEVASGPKPWLTIIVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180
           YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFL+SGMSPRVAIFFFSFATIKT
Sbjct: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLISGMSPRVAIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240
           VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240

Query: 241 RAGGGFEARVKED 254
           RAGGGFEARVKED
Sbjct: 241 RAGGGFEARVKED 253

BLAST of CsaV3_5G032610 vs. ExPASy TrEMBL
Match: A0A6J1JJ91 (sphinganine C4-monooxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485096 PE=3 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 1.1e-136
Identity = 241/253 (95.26%), Postives = 248/253 (98.02%), Query Frame = 0

Query: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60
           MGIVISDQILGTFVPI+VYWLYSG+Y+LLGSF+NYRLHSKKDE EKNLVSKSTV+RGVF 
Sbjct: 1   MGIVISDQILGTFVPILVYWLYSGIYVLLGSFDNYRLHSKKDEQEKNLVSKSTVLRGVFF 60

Query: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQ IQAIVAILLFKVTE +GEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQTIQAIVAILLFKVTENEGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180
           YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT
Sbjct: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240
           VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTY PYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYRPYSLEK 240

Query: 241 RAGGGFEARVKED 254
           RAGGGFEAR+KED
Sbjct: 241 RAGGGFEARLKED 253

BLAST of CsaV3_5G032610 vs. TAIR 10
Match: AT1G14290.1 (sphingoid base hydroxylase 2 )

HSP 1 Score: 409.5 bits (1051), Expect = 2.0e-114
Identity = 195/250 (78.00%), Postives = 220/250 (88.00%), Query Frame = 0

Query: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60
           M  VISD+ LGTFVPI+VYW+YSG+YI LGS + YRLHSK DE EKNLVSKS VV+GV L
Sbjct: 2   MSFVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLL 61

Query: 61  QQIIQAIVAILLFKVTETDGEVATGPK-SWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKF 120
           QQ +QAI++++LFK+T +D + AT  + S L +  QFI+AMLV+DTWQYFIHRYMH NKF
Sbjct: 62  QQTLQAIISVILFKITGSDADAATTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNKF 121

Query: 121 LYKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIK 180
           LYKH+HSQHHRL+VPY++GALYNHPLEGLLLDTIGGALSFL SGMSPR AIFFFSFATIK
Sbjct: 122 LYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFATIK 181

Query: 181 TVDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLE 240
           TVDDHCGLWLPGN FH+FF NNSAYHDVHHQLYG+KYNFSQPFFV WDRI GTY+PYSLE
Sbjct: 182 TVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWDRILGTYLPYSLE 241

Query: 241 KRAGGGFEAR 250
           KRA GGFE R
Sbjct: 242 KRANGGFETR 251

BLAST of CsaV3_5G032610 vs. TAIR 10
Match: AT1G69640.1 (sphingoid base hydroxylase 1 )

HSP 1 Score: 408.7 bits (1049), Expect = 3.5e-114
Identity = 192/250 (76.80%), Postives = 221/250 (88.40%), Query Frame = 0

Query: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60
           MG  +SD++LGT  PIVVYWLYSG+Y+ L S E+YRLHSK +E EKNLVSKS+VV+GV +
Sbjct: 3   MGFAVSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLV 62

Query: 61  QQIIQAIVAILLFKVTETDGEVATGPK-SWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKF 120
           QQ++QA+VAILLF VT +D E     + S+L +  QF+ AM+VLDTWQYF+HRYMHQNKF
Sbjct: 63  QQVVQAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKF 122

Query: 121 LYKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIK 180
           LYKH+HSQHHRL+VPYA+GALYNHP+EGLLLDTIGGALSFLVSGMSPR +IFFFSFATIK
Sbjct: 123 LYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIK 182

Query: 181 TVDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLE 240
           TVDDHCGLWLPGNLFH+ F+NNSAYHD+HHQLYG+KYNFSQPFFV WDRI GTYMPYSLE
Sbjct: 183 TVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLE 242

Query: 241 KRAGGGFEAR 250
           KR  GGFEAR
Sbjct: 243 KREDGGFEAR 252

BLAST of CsaV3_5G032610 vs. TAIR 10
Match: AT2G29390.3 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 72.8 bits (177), Expect = 4.5e-13
Identity = 68/238 (28.57%), Postives = 105/238 (44.12%), Query Frame = 0

Query: 10  LGTFVPIVVYWLYSGL-YILL---GSFENYRLHSKKDELEKNLVSKSTVVRGVFLQQIIQ 69
           +G+F+     +  SGL YI L   G   NY++ +K +  E        + R +     + 
Sbjct: 17  IGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARLLLYHCCVN 76

Query: 70  AIVAILLFKVTETDGEVATGP-KSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFLYKHV 129
             + +  + V    G  ++ P  SW  +  Q +   ++ D   Y+ HR +H  K+LYK+V
Sbjct: 77  LPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRILH-TKWLYKNV 136

Query: 130 HSQHHRLVVPYAFGALYNHPLEGLLL---DTIGGALSFLVSGMSPRVAIFFFSFATIKTV 189
           HS HH    P+   + Y HP E L L     +G AL    +G        +     I+TV
Sbjct: 137 HSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAL----TGPHLITLWLWMMLRVIETV 196

Query: 190 DDHCGL---WLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPY 237
           + HCG    W P N   ++    + +HD HH+L  +K       FV  D IFGT   Y
Sbjct: 197 EAHCGYHFPWSPSNFLPLY--GGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGY 244

BLAST of CsaV3_5G032610 vs. TAIR 10
Match: AT2G29390.2 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 72.8 bits (177), Expect = 4.5e-13
Identity = 68/238 (28.57%), Postives = 105/238 (44.12%), Query Frame = 0

Query: 10  LGTFVPIVVYWLYSGL-YILL---GSFENYRLHSKKDELEKNLVSKSTVVRGVFLQQIIQ 69
           +G+F+     +  SGL YI L   G   NY++ +K +  E        + R +     + 
Sbjct: 24  IGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARLLLYHCCVN 83

Query: 70  AIVAILLFKVTETDGEVATGP-KSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFLYKHV 129
             + +  + V    G  ++ P  SW  +  Q +   ++ D   Y+ HR +H  K+LYK+V
Sbjct: 84  LPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRILH-TKWLYKNV 143

Query: 130 HSQHHRLVVPYAFGALYNHPLEGLLL---DTIGGALSFLVSGMSPRVAIFFFSFATIKTV 189
           HS HH    P+   + Y HP E L L     +G AL    +G        +     I+TV
Sbjct: 144 HSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAL----TGPHLITLWLWMMLRVIETV 203

Query: 190 DDHCGL---WLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPY 237
           + HCG    W P N   ++    + +HD HH+L  +K       FV  D IFGT   Y
Sbjct: 204 EAHCGYHFPWSPSNFLPLY--GGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGY 251

BLAST of CsaV3_5G032610 vs. TAIR 10
Match: AT2G29390.1 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 71.2 bits (173), Expect = 1.3e-12
Identity = 69/248 (27.82%), Postives = 107/248 (43.15%), Query Frame = 0

Query: 10  LGTFVPIVVYWLYSGL-YILL---GSFENYRLHSKKDELEKNLVSKSTVVRGVFLQQIIQ 69
           +G+F+     +  SGL YI L   G   NY++ +K +  E        + R +     + 
Sbjct: 24  IGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARLLLYHCCVN 83

Query: 70  AIVAILLFKVTETDGEVATGP-KSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFLYKHV 129
             + +  + V    G  ++ P  SW  +  Q +   ++ D   Y+ HR +H  K+LYK+V
Sbjct: 84  LPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRILH-TKWLYKNV 143

Query: 130 HSQHHRLVVPYAFGALYNHPLEGLLL---DTIGGALSFLVSGMSPRVAIFFFSFATIKTV 189
           HS HH    P+   + Y HP E L L     +G AL    +G        +     I+TV
Sbjct: 144 HSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAL----TGPHLITLWLWMMLRVIETV 203

Query: 190 DDHCGL---WLPGNLFHVF----------FRNNSAYHDVHHQLYGSKYNFSQPFFVTWDR 237
           + HCG    W P N   ++          F  ++ +HD HH+L  +K       FV  D 
Sbjct: 204 EAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLLYTKSGNYSSTFVYMDW 263

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004135460.11.4e-141100.00sphinganine C4-monooxygenase 2 [Cucumis sativus] >KGN51908.1 hypothetical protei... [more]
XP_008446343.14.1e-14199.60PREDICTED: sphinganine C4-monooxygenase 2 [Cucumis melo] >KAA0034409.1 sphingani... [more]
XP_022151262.14.7e-13796.05sphinganine C4-monooxygenase 2-like [Momordica charantia][more]
XP_022987574.12.3e-13695.26sphinganine C4-monooxygenase 2-like [Cucurbita maxima] >KAG7032210.1 Sphinganine... [more]
XP_038892349.13.0e-13696.05sphinganine C4-monooxygenase 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9AST32.9e-11378.00Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=SBH2 PE=1 SV=1[more]
Q8VYI14.9e-11376.80Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=SBH1 PE=1 SV=1[more]
B8B4W42.3e-9465.08Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. indica OX=39... [more]
Q0D4G32.3e-9465.08Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. japonica OX=... [more]
O942982.5e-5343.08Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
Match NameE-valueIdentityDescription
A0A0A0KQL66.8e-142100.00Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C... [more]
A0A5A7SZ772.0e-14199.60Sphinganine C4-monooxygenase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3BEC52.0e-14199.60sphinganine C4-monooxygenase 2 OS=Cucumis melo OX=3656 GN=LOC103489111 PE=3 SV=1[more]
A0A6J1DD012.3e-13796.05sphinganine C4-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC1110192... [more]
A0A6J1JJ911.1e-13695.26sphinganine C4-monooxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485096 ... [more]
Match NameE-valueIdentityDescription
AT1G14290.12.0e-11478.00sphingoid base hydroxylase 2 [more]
AT1G69640.13.5e-11476.80sphingoid base hydroxylase 1 [more]
AT2G29390.34.5e-1328.57sterol 4-alpha-methyl-oxidase 2-2 [more]
AT2G29390.24.5e-1328.57sterol 4-alpha-methyl-oxidase 2-2 [more]
AT2G29390.11.3e-1227.82sterol 4-alpha-methyl-oxidase 2-2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 96..232
e-value: 3.9E-26
score: 91.9
NoneNo IPR availablePANTHERPTHR11863:SF122SPHINGANINE C4-MONOOXYGENASE 1-LIKEcoord: 3..249
NoneNo IPR availablePANTHERPTHR11863STEROL DESATURASEcoord: 3..249

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_5G032610.1CsaV3_5G032610.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008610 lipid biosynthetic process
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity