CsaV3_5G032350 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_5G032350
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Descriptionprotein NRT1/ PTR FAMILY 4.6-like
Locationchr5: 26194206 .. 26200525 (+)
RNA-Seq ExpressionCsaV3_5G032350
SyntenyCsaV3_5G032350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTGGGTTTAAGTGATGTGGGTCAAAGAAAGAAAAGGATACCCAAATTAAATTTTGTTTTTTGTTTTATTGAGTGGAGGGATTTGATGCTTCTATATCTACCTGTTTCTTTCTTCTCTTCTTTTATGCCAAACCCAAAGTAAACCCTTTTTTTAACACACAACACACAAAAGAATCCAAACTTAAGTTTCTCTCCTCCCCGTCCCTAACCCCATGTGCATCTCTCTCTCTGTCTCTCTCTATATAAGAGTGCCTTATAGAGAATTTTATTTATAAAGAAAAACATATATATTATTCTTTGTGGCAAAATGAAGTGGTTTAATTAATTAATTTTGTGAAGAATGGAAGTCATTTTCTCTTCTCTCTGATCTCTCTTTCTTATAATTTTTTTTTAATCTTTTGTCTCATATCCCTCCCTCGACCCTTTCTCCCTCTCCCTATAAACTCTTGAAATCTCCCACTCCACTTCTCCCCTCTCACTTCTCTTTACAATAACCAAAACCCATCATCACAAAAGAGCTTCTAAACCAATAATATGGTACTGTTTCTCTCCCTCAGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCATATTCTATTTCTATTCAAAATTTCATTCATCATTTATTCTAAATGGGTTTTGTTTCGTTTTGTTTTCTTGGTAGGAAATTGGTGATGATCATGGTCGAACTAATAGATGGGAAGGATATGTAGATTGGAGAAAGAAACCAGCTTTGAGAGGTCGCCATGGAGGAATCATTGCAGCATCTTTTGATTTGGGTATGATTAATGTTATTATGAAGTAATTATAAATTGAAACCCTGTTGGTTTTTAGAGGTTTGATTAATTGATTATTAATTAATTATATTATGTATATATGTATGTATGTAGTTGTGGAAGTATTGGAGAATTTGGCGTTTTTAGCAAATGCAAGCAACTTGGTGATGTACTTATCCCATTACATGCACTTTTCACCATCAAAATCAGCAAACAATGTCACAGATTTCATGGGAACAGCCTTCCTTCTTGCCCTTCTTGGTGGTTTCCTTTCTGATGCCTTTTTCACTTCTTATCATATCTACCTCATTAGTGCTGCCATTCAATTACTTGTAAGTTTTTTTTTCTTTTCTTTCCTTAATATTATTTAACTAAGAAGGAAGATATTTTAATTTAATTTATATATTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATCTGTTTAATTAATTAAACTAAAAGAAAAGGGGAGAGTTGAATTGGAGAGGATGTGGGATGATCACGAGCTAACTGTTGAATCCCCCCCATGGTTGTAAAGGGAAAAGCAAGGGAGAAAACTTCAGGGAATTAAATTTGCTTATTAATTTCGTCGGTCCTCCCTCCCTCTACATTTTTTTTTCTTTTTTGGTTTCTACATTTAACATTTTATCTTTCAAATTTGAAATTTGATTTCATTTTTTTATTTCTGTGTTTTAAGACTTTCAATTCTACTTTTAGTTATTTGATACCATTGTCTCTATTAGTTAATTCAAAATAAATAATTACAAAGTAAAATTTTAAATTTCCCTTTAATAGTAAGTGATTATTTAATCACCTCTGTTTATTTTAAATTAATTAATAGAAATTAACCTATATTTAAAATACAAATTAAATATTTAATTAAAAATAGAAAAAATTCTAACCTTTTTAAAATATTACAAAAATTAAATTGAAACTAAACTTAAATCCTAAAAAAGATTCAAAATTAAAAGATTATTTATAAACTAAATTCAAAATTTAAGAACATGAAATATACTTTTCATTTAAAACAAGTTTAACTGTTTAACTCGCTCATATTAGTAATTACGATGAAATCTTTTTATTAGATTAGGAAGATCGAATCATGATCATGCAAAGTGTGTGCGTGCGTGTGGTACATTTCTTCATAAATAAAATATTTAATATTTAAATAAAAGAACATTTTACTTGAAAATTTTCAAATATAATAAATTTCGTCTTTTAGTAATTTAACGATAAAAAATATATTGTTTTACCTTGGTATTGTGTTTACTCTAAGTATTGTCCCTATTCGTACGCTATTGGTCCTTAACCCGAAGGGATCTTTTCCAATGAAGGAGTGCCACGGGGATGACACTTCTGTTTTCTATTAGGACACGTGGCCTTATGTCTCGTCCACATCGCTCAGAAATTTATTGACACACAATTTTCTTTTTCTGTACAGTTGTCCATTTATGGTCTTTGTCTAAGATTTGTTGCCCACTTCCAAATATCAAATGTCCTCTTATTCATCTCTTGTGGCCCTTCCTTATTTCCCTAGTTGGTATACCTTTGATTCTTCATTAATGCCATTGCCAACTATCTTTCCTTCATTCTTCTTTCCCCAGTTACTAACTCAACTGTCATTGATATGTATTTCTTTCTTTCCAAGTTTAGCGTTTAAATCTTTAGACACATTCGTTGGATGGGTATGTGCTCAAATGAAATAGAAATATACTCCTGATTAATATAAGTAGACGTACTTGATCAAGCTTTCATTTTTGTTTATGCATTTAGAGAGTAATTATGTGAGGTGTAGGGTATGATTATTTTTAATGTTTTTTTGTTTAGAAAAATAGGGATAAGTTGAAAACTTGATTATTTAAGAGATAGGGTATGACGTTGTGGATTAATTTATCATTACTGAAATATGATTAAATAATTGGGGTGAATTGACTCAACCATACTCTATGATAAGGAATCCTTTGAAGACTTTAAAAAATGTATGAATATTTTAAACTAAGTGCTTATCTTCAAATGCACATTAGGATACAAATTCAAAATTGAATTAAAAAAGCAACAACATTGGATTCATGGATGAACATCATGATGATTCTTTGTGAATCATTATATAAAGTTAAGTAACTTTCAAAAACGAATTGTTTTTGTTAATTATTATAAAGATATATATACCTAAATTAATTAATATTTAAAAAAAAATGGTAAAAATGAATTCTAAAATATCATAATGATATACAATTAGGTATTATGATATTAAATTTGAAGCAGTCCTTATCAATTGATATGAGAGTTATGGGTAAGGATTGGGGGTATGAAAAAGAAATATGATAAACATAGATGAGAGAATGGCGAGTTGAAGAGAGGGATTTGAAGGGAGTAAAGTTTTATAATAAAATGAAAATAGTTATTATATGGTATAGAAATGAAGAGAGAAAAGGAAGCATATTTTATAAAACGCGAGATTACATGGTCAAAACGACATAAAAAATTTAAAAGAAGGAAAAGGAAAAGATGTCCGTATAAACAGGTAAGCGGCAACCAATAAGGTGAGAAGGAAAATGTAACACGTGGCAACAAGAGTGTACACGTAAGTGTGAGTACATATTATTATGATGAGAGAATATGATATGTTTAGTACTGACACATGGCAGATTGCATTAACTACTTTACTTACTACTTACTGACTCACTCACTCACTATCCTATCATTTCAATTTTATTTATTCTCATATTCGATTACCTCTTTTATTGGGTCGGCTTTCTTATTATAACGTCCCTACTCGATCATCCCACAACTTACTTTATTCTAATTTTGCTGCCTACCTTTTACTACGCCTTTTCCCTTAATAATGCTCCATTTTCTGTCCTTTTCTTGCATTTTTGTGAGAATGTTGTATGATATGTTATGGTGAGGTCAGAAGTAGTTAGTATTCTAACACGATTAAAAAAGCCATATTGATCGAGTCGATGATAATAGTAATAAGATCTAACTGTGTCGTCATACAACTCTACTTTTCTTATCCTTGGCCTCAGTCACTTTTTTGCTTCTTCTGTTTTTTATAGTTTCTTGTACAATATGCAAAATCTAAAGTCAAGCATTTGAGCAGGGGTTGACAGTATTGTTGATACAAGCCAAGATGCCATCGCTGATGCCTCCTCCATGTGATCGCCAAGCAAATCATAACGCCGTGTGCGAAGAAGCGAGTGGTGGCAAAGCAGCAATGCTGTTCGCGGGACTATATCTGGTGGCATTAGGGGTTGGTGGCATAAAGGGGTCGTTGCCATCACATGGGGCGGAGCAGTTTGATGACAGTACCCCACAGGGCAGGAAAAGAAGATCCACATTTTTCAACTACTTCGTCTTTTGCCTCTCATGTGGTGGCCTAATTGCAGTGACATTGGTGGTGTGGGTTGAAGACAACAAAGGGTGGGAGTGGGGCTTTGGAATTGCAATGCTCAGCATATTTTTGTCCATCATTGTCTTCTTTGCGGGATCCCCTCTATACCGTAATAAAATACCTGATGGAAGTCCATTCACAACAATTAGCAAGGTATGTAGAAGTATAATTGACCATAGAAATGAAGTTAAAGATATTAAGAGTGGGGGAAAGTTAACTGAAACAACCTTTTTGTTTTTTAACTGAGCAGGTTTTAGTTGCTGCAACGTTTGGTTGCTGCATCGGCACGAACTCACGAAACTCAATAGCGAGTATGGCAATGAGCCCGGCTCTTGATGACAAGGAAGCTAAACAGAATGCAAAAGAAATAGATACAACAGATCACATGATGAACGAACCGTCAGAGAGTCTGAAGTTTCTAAATAATGCAGTTCTGAACAAGCCATTCCATTCAGCATTAGAATGTACAGTTAAAGAAGTTGAAGAGGTCAAGATTGTTCTGAGAATTCTACCAATATTTGCTTGTACCATCATCCTCAACTGTTGTGTAGCTCAGCTCTCCACATTTTCGGTTGAACAAGCATCAACAATGGACACCAAAATAGGTTCCTTTAAAGTTCCACCTGCATCTCTCCCCATTTTCCCTATACTCTTCATCATGTTCCTCGCCCCGATATACGATCACTTAATCATCCCATTTTCTCGAAGAATAACTAAAACCGAAACAGGAATCACTCATCTTCAGAGGATAGGAGTTGGGTTACTTTTCTCTATTGGAGCCATGGCAGTTGCAGCAGCTGTGGAGACTAAGCGTAAGAGAGTAGCCACTGAAAACGGTCTACTCGACTCCGCTCAACCGTTGCCCATCACATTCCTTTGGATTGCCATCCAGTACTTGTTTCTCGGATCAGCAGATCTTTTCTCCTTGGCTGGATCATTAGAATTTTTCTTCACAGAAGCACCTGCAAGCATGAGATCCTTGGCTACATCTCTCTCCTGGGCTTCTCTGGCTATGGGTTACTACCTTAGTTCAGTTATCGTATCAATAGTCAACAATGTCACAGATCACTCTGACCACCAACCATGGCTATCCGGAAGAAACATAAACCATTATCACCTTGAGAGATTCTACTGGTTGATGTGTGTGCTCAGTGGATTAAACTTTGTGCATTACCTATTTTGGGCCATGCAGTACAAGTACAGACCAAAATCACAACAATAGGTGAAATAACCACAGAGACACAAATATATATATGATGCAATAATACTTACACGGTTCCACACATTTATTCAAACAAATTGAAATTATCACCCCCATCCAGAGGAGAACAGAAACTGACAAGCTCTGCAAATGCTCTCATCATCTTTCAGCTGGCATGGGATTAAATGGGAGGTTAAATCCTTCCTCTTGGCAGAGCAGGTAGATGTGGTAACTTGCATGTGAAATGTCTCATATCAGTGAATTTCTGTGATGCAAAGCATCTTTTTATCAATCTCTTGTGTTTGGTTTCAAGTAAAATTCAACCTTCTTGTTGCCATGTCATTAGATGAACAACAATAACAAAATGGTAATTTTAATCATAATCCATGAGTCTGCAATAATCTTAAGCAAGAAAATTTGCAACCTAGGGAGGTGAAGAGATGGATCAACAAGTTATTGAAAAGTGACCTTTCATGCCATGTATTATCCAATGATTCAATACATGGATTTATCTGAAAGCTCACAGTCACAGTCTATACCTTCAAGACGCGTCGAATCACTTTTCGAAATCATTTGAATTAGAAAAAACTATGAACCAAAATTTTGTTAGGTTCACAAGGACTTAACTGAAATTTGATGCTTCTAAATCTACCTCCATTCACACATGGCATGCATAGGACTTGAATAACTTTGTGGCGATGTTCGTTTGTGGAGTCTTTCTGGCTTTGTAGGTTTAAGACCTTTGGTGTTTCTTTGCATTGAAATGGCGATGATTGCTATTCTTGAGAAGGTAATCATGAGTCCGTATGTTTGGGAGAAAAATGATTTGTTATGTCGTTATATTGTCTCTTTCATTTCTCTTTATCAAACTTCAGTTTCTTAGAGA

mRNA sequence

ATGGAAATTGGTGATGATCATGGTCGAACTAATAGATGGGAAGGATATGTAGATTGGAGAAAGAAACCAGCTTTGAGAGGTCGCCATGGAGGAATCATTGCAGCATCTTTTGATTTGGTTGTGGAAGTATTGGAGAATTTGGCGTTTTTAGCAAATGCAAGCAACTTGGTGATGTACTTATCCCATTACATGCACTTTTCACCATCAAAATCAGCAAACAATGTCACAGATTTCATGGGAACAGCCTTCCTTCTTGCCCTTCTTGGTGGTTTCCTTTCTGATGCCTTTTTCACTTCTTATCATATCTACCTCATTAGTGCTGCCATTCAATTACTTGGGTTGACAGTATTGTTGATACAAGCCAAGATGCCATCGCTGATGCCTCCTCCATGTGATCGCCAAGCAAATCATAACGCCGTGTGCGAAGAAGCGAGTGGTGGCAAAGCAGCAATGCTGTTCGCGGGACTATATCTGGTGGCATTAGGGGTTGGTGGCATAAAGGGGTCGTTGCCATCACATGGGGCGGAGCAGTTTGATGACAGTACCCCACAGGGCAGGAAAAGAAGATCCACATTTTTCAACTACTTCGTCTTTTGCCTCTCATGTGGTGGCCTAATTGCAGTGACATTGGTGGTGTGGGTTGAAGACAACAAAGGGTGGGAGTGGGGCTTTGGAATTGCAATGCTCAGCATATTTTTGTCCATCATTGTCTTCTTTGCGGGATCCCCTCTATACCGTAATAAAATACCTGATGGAAGTCCATTCACAACAATTAGCAAGGTTTTAGTTGCTGCAACGTTTGGTTGCTGCATCGGCACGAACTCACGAAACTCAATAGCGAGTATGGCAATGAGCCCGGCTCTTGATGACAAGGAAGCTAAACAGAATGCAAAAGAAATAGATACAACAGATCACATGATGAACGAACCGTCAGAGAGTCTGAAGTTTCTAAATAATGCAGTTCTGAACAAGCCATTCCATTCAGCATTAGAATGTACAGTTAAAGAAGTTGAAGAGGTCAAGATTGTTCTGAGAATTCTACCAATATTTGCTTGTACCATCATCCTCAACTGTTGTGTAGCTCAGCTCTCCACATTTTCGGTTGAACAAGCATCAACAATGGACACCAAAATAGGTTCCTTTAAAGTTCCACCTGCATCTCTCCCCATTTTCCCTATACTCTTCATCATGTTCCTCGCCCCGATATACGATCACTTAATCATCCCATTTTCTCGAAGAATAACTAAAACCGAAACAGGAATCACTCATCTTCAGAGGATAGGAGTTGGGTTACTTTTCTCTATTGGAGCCATGGCAGTTGCAGCAGCTGTGGAGACTAAGCGTAAGAGAGTAGCCACTGAAAACGGTCTACTCGACTCCGCTCAACCGTTGCCCATCACATTCCTTTGGATTGCCATCCAGTACTTGTTTCTCGGATCAGCAGATCTTTTCTCCTTGGCTGGATCATTAGAATTTTTCTTCACAGAAGCACCTGCAAGCATGAGATCCTTGGCTACATCTCTCTCCTGGGCTTCTCTGGCTATGGGTTACTACCTTAGTTCAGTTATCGTATCAATAGTCAACAATGTCACAGATCACTCTGACCACCAACCATGGCTATCCGGAAGAAACATAAACCATTATCACCTTGAGAGATTCTACTGGTTGATGTGTGTGCTCAGTGGATTAAACTTTGTGCATTACCTATTTTGGGCCATGCAGTACAAGTACAGACCAAAATCACAACAATAG

Coding sequence (CDS)

ATGGAAATTGGTGATGATCATGGTCGAACTAATAGATGGGAAGGATATGTAGATTGGAGAAAGAAACCAGCTTTGAGAGGTCGCCATGGAGGAATCATTGCAGCATCTTTTGATTTGGTTGTGGAAGTATTGGAGAATTTGGCGTTTTTAGCAAATGCAAGCAACTTGGTGATGTACTTATCCCATTACATGCACTTTTCACCATCAAAATCAGCAAACAATGTCACAGATTTCATGGGAACAGCCTTCCTTCTTGCCCTTCTTGGTGGTTTCCTTTCTGATGCCTTTTTCACTTCTTATCATATCTACCTCATTAGTGCTGCCATTCAATTACTTGGGTTGACAGTATTGTTGATACAAGCCAAGATGCCATCGCTGATGCCTCCTCCATGTGATCGCCAAGCAAATCATAACGCCGTGTGCGAAGAAGCGAGTGGTGGCAAAGCAGCAATGCTGTTCGCGGGACTATATCTGGTGGCATTAGGGGTTGGTGGCATAAAGGGGTCGTTGCCATCACATGGGGCGGAGCAGTTTGATGACAGTACCCCACAGGGCAGGAAAAGAAGATCCACATTTTTCAACTACTTCGTCTTTTGCCTCTCATGTGGTGGCCTAATTGCAGTGACATTGGTGGTGTGGGTTGAAGACAACAAAGGGTGGGAGTGGGGCTTTGGAATTGCAATGCTCAGCATATTTTTGTCCATCATTGTCTTCTTTGCGGGATCCCCTCTATACCGTAATAAAATACCTGATGGAAGTCCATTCACAACAATTAGCAAGGTTTTAGTTGCTGCAACGTTTGGTTGCTGCATCGGCACGAACTCACGAAACTCAATAGCGAGTATGGCAATGAGCCCGGCTCTTGATGACAAGGAAGCTAAACAGAATGCAAAAGAAATAGATACAACAGATCACATGATGAACGAACCGTCAGAGAGTCTGAAGTTTCTAAATAATGCAGTTCTGAACAAGCCATTCCATTCAGCATTAGAATGTACAGTTAAAGAAGTTGAAGAGGTCAAGATTGTTCTGAGAATTCTACCAATATTTGCTTGTACCATCATCCTCAACTGTTGTGTAGCTCAGCTCTCCACATTTTCGGTTGAACAAGCATCAACAATGGACACCAAAATAGGTTCCTTTAAAGTTCCACCTGCATCTCTCCCCATTTTCCCTATACTCTTCATCATGTTCCTCGCCCCGATATACGATCACTTAATCATCCCATTTTCTCGAAGAATAACTAAAACCGAAACAGGAATCACTCATCTTCAGAGGATAGGAGTTGGGTTACTTTTCTCTATTGGAGCCATGGCAGTTGCAGCAGCTGTGGAGACTAAGCGTAAGAGAGTAGCCACTGAAAACGGTCTACTCGACTCCGCTCAACCGTTGCCCATCACATTCCTTTGGATTGCCATCCAGTACTTGTTTCTCGGATCAGCAGATCTTTTCTCCTTGGCTGGATCATTAGAATTTTTCTTCACAGAAGCACCTGCAAGCATGAGATCCTTGGCTACATCTCTCTCCTGGGCTTCTCTGGCTATGGGTTACTACCTTAGTTCAGTTATCGTATCAATAGTCAACAATGTCACAGATCACTCTGACCACCAACCATGGCTATCCGGAAGAAACATAAACCATTATCACCTTGAGAGATTCTACTGGTTGATGTGTGTGCTCAGTGGATTAAACTTTGTGCATTACCTATTTTGGGCCATGCAGTACAAGTACAGACCAAAATCACAACAATAG

Protein sequence

MEIGDDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYLSHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQAKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDSTPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFAGSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEIDTTDHMMNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ*
Homology
BLAST of CsaV3_5G032350 vs. NCBI nr
Match: XP_011655668.1 (protein NRT1/ PTR FAMILY 4.6 [Cucumis sativus] >ADQ74764.1 nitrate transporter 1.2a [Cucumis sativus] >KGN51883.1 hypothetical protein Csa_009160 [Cucumis sativus])

HSP 1 Score: 1151.7 bits (2978), Expect = 0.0e+00
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 1   MEIGDDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYL 60
           MEIGDDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYL
Sbjct: 1   MEIGDDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYL 60

Query: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQ 120
           SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQ
Sbjct: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQ 120

Query: 121 AKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180
           AKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD
Sbjct: 121 AKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180

Query: 181 STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA 240
           STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA
Sbjct: 181 STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA 240

Query: 241 GSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEI 300
           GSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEI
Sbjct: 241 GSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEI 300

Query: 301 DTTDHMMNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360
           DTTDHMMNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCV
Sbjct: 301 DTTDHMMNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360

Query: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG 420
           AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG
Sbjct: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG 420

Query: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGS 480
           ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGS
Sbjct: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGS 480

Query: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWL 540
           ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWL
Sbjct: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWL 540

Query: 541 SGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 584
           SGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ
Sbjct: 541 SGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 583

BLAST of CsaV3_5G032350 vs. NCBI nr
Match: XP_038893294.1 (protein NRT1/ PTR FAMILY 4.6-like [Benincasa hispida])

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 561/583 (96.23%), Postives = 572/583 (98.11%), Query Frame = 0

Query: 1   MEIGDDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYL 60
           ME GDD  +  RWEGYVDWRKKPALRGRHGGIIAASFDLVVE+LENLAFLANASNLVMYL
Sbjct: 1   MESGDD--QIGRWEGYVDWRKKPALRGRHGGIIAASFDLVVEILENLAFLANASNLVMYL 60

Query: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQ 120
           SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGL VLLIQ
Sbjct: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLAVLLIQ 120

Query: 121 AKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180
           AKMPSLMPPPCD QANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD
Sbjct: 121 AKMPSLMPPPCDHQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180

Query: 181 STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA 240
           STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA
Sbjct: 181 STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA 240

Query: 241 GSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEI 300
           GSPLYRNKIPDGSPFTTISKVLVAATFGCCI TNSRNSIASMAMSPALDDKEAKQ AKE 
Sbjct: 241 GSPLYRNKIPDGSPFTTISKVLVAATFGCCISTNSRNSIASMAMSPALDDKEAKQKAKER 300

Query: 301 DTTDHMMNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360
            +T+H++++PSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCV
Sbjct: 301 GSTNHVVDKPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360

Query: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG 420
           AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG
Sbjct: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG 420

Query: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGS 480
           ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGS
Sbjct: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGS 480

Query: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWL 540
           ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTD+SDH+PWL
Sbjct: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDNSDHKPWL 540

Query: 541 SGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 584
           SGRNINHYHLERFYWLMCVLSGLNFVHYLFWAM+YKYRPKSQQ
Sbjct: 541 SGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKSQQ 581

BLAST of CsaV3_5G032350 vs. NCBI nr
Match: XP_023537771.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.5e-303
Identity = 533/583 (91.42%), Postives = 558/583 (95.71%), Query Frame = 0

Query: 1   MEIGDDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYL 60
           ME GD   R +RWEGYVDWRK+PAL+GRHGGIIAA+FDLVVE+LENLAFLANASNLVMYL
Sbjct: 1   MESGD---RVSRWEGYVDWRKRPALKGRHGGIIAAAFDLVVEILENLAFLANASNLVMYL 60

Query: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQ 120
           SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSY IYLISAAIQLLGL VLLIQ
Sbjct: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYLIYLISAAIQLLGLAVLLIQ 120

Query: 121 AKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180
           AK+PSLMPPPCDR ANH  +CEEASGGK AMLF+GLYLVALGVGGIKGSLPSHGAEQFDD
Sbjct: 121 AKIPSLMPPPCDRHANHGTMCEEASGGKEAMLFSGLYLVALGVGGIKGSLPSHGAEQFDD 180

Query: 181 STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA 240
           STP GRKRRS+FFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSI LS++VFFA
Sbjct: 181 STPHGRKRRSSFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIVLSMVVFFA 240

Query: 241 GSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEI 300
           GSPLYRNKIP+GSPFTTISKVLVAA FGCCIG NSRNSI+SM MSPALDDKEA QN KE 
Sbjct: 241 GSPLYRNKIPNGSPFTTISKVLVAAMFGCCIGMNSRNSISSMEMSPALDDKEAIQNEKER 300

Query: 301 DTTDHMMNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360
            +TD  + EPSESLKFLNNA LNKPF+SALECTVKEVEEVKIVLRILPIFACTIILNCCV
Sbjct: 301 HSTDE-VEEPSESLKFLNNAALNKPFYSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360

Query: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG 420
           AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG
Sbjct: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG 420

Query: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGS 480
           ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVAT++GLLDSAQPLPI+FLWIAIQYLFLGS
Sbjct: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATKHGLLDSAQPLPISFLWIAIQYLFLGS 480

Query: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWL 540
           ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSV++SI+NNVTD SDHQPWL
Sbjct: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVVISIINNVTDRSDHQPWL 540

Query: 541 SGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 584
           SGRN+NHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPK++Q
Sbjct: 541 SGRNLNHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKARQ 579

BLAST of CsaV3_5G032350 vs. NCBI nr
Match: KAG6601408.1 (Protein NRT1/ PTR FAMILY 4.6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1051.2 bits (2717), Expect = 3.3e-303
Identity = 533/583 (91.42%), Postives = 558/583 (95.71%), Query Frame = 0

Query: 1   MEIGDDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYL 60
           ME GD   R +RWEGYVDWRK+PAL+GRHGGIIAA+FDLVVE+LENLAFLANASNLVMYL
Sbjct: 1   MESGD---RVSRWEGYVDWRKRPALKGRHGGIIAAAFDLVVEILENLAFLANASNLVMYL 60

Query: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQ 120
           SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSY IYLISAAIQLLGL VLLIQ
Sbjct: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYLIYLISAAIQLLGLAVLLIQ 120

Query: 121 AKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180
           AK+PSLMPPPCDR ANH  +C+EASGGK AMLFAGLYLVALGVGGIKGSLPSHGAEQFDD
Sbjct: 121 AKIPSLMPPPCDRHANHGTMCQEASGGKEAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180

Query: 181 STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA 240
           STP GRKRRS+FFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSI LS+IVFFA
Sbjct: 181 STPHGRKRRSSFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIVLSMIVFFA 240

Query: 241 GSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEI 300
           GSPLYRNKIP+GSPFTTISKVLVAA FGCCIG NSRNSI+SM MSPALDDKEA QN KE 
Sbjct: 241 GSPLYRNKIPNGSPFTTISKVLVAAMFGCCIGMNSRNSISSMEMSPALDDKEAIQNEKER 300

Query: 301 DTTDHMMNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360
            +TD  + EPSESLKFLNNA LNKPF+SALECTVKEVEEVKIVLRILPIFACTIILNCCV
Sbjct: 301 HSTDE-VEEPSESLKFLNNAALNKPFYSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360

Query: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG 420
           AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDH+IIPFSRRITKTETG
Sbjct: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIIIPFSRRITKTETG 420

Query: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGS 480
           ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVAT++GLLDSAQPLPI+FLWIAIQYLFLGS
Sbjct: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATKHGLLDSAQPLPISFLWIAIQYLFLGS 480

Query: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWL 540
           ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSV++SI+NNVTD SDHQPWL
Sbjct: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVVISIINNVTDRSDHQPWL 540

Query: 541 SGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 584
           SGRN+NHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPK++Q
Sbjct: 541 SGRNLNHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKARQ 579

BLAST of CsaV3_5G032350 vs. NCBI nr
Match: XP_022998594.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita maxima])

HSP 1 Score: 1050.0 bits (2714), Expect = 7.3e-303
Identity = 534/583 (91.60%), Postives = 556/583 (95.37%), Query Frame = 0

Query: 1   MEIGDDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYL 60
           ME GD   R +RWEGYVDWRK+PAL+GRHGGIIAA+FDLVVE+LENLAFLANASNLVMYL
Sbjct: 1   MESGD---RVSRWEGYVDWRKRPALKGRHGGIIAAAFDLVVEILENLAFLANASNLVMYL 60

Query: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQ 120
           SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSY IY ISAAIQLLGL VLLIQ
Sbjct: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYLIYFISAAIQLLGLAVLLIQ 120

Query: 121 AKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180
           AK+PSLMPPPCDR ANH  +C+EASGGK AMLFAGLYLVALGVGGIKGSLPSHGAEQFDD
Sbjct: 121 AKIPSLMPPPCDRHANHGTMCQEASGGKEAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180

Query: 181 STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA 240
           STP GRKRRS+FFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSI LS+IVFFA
Sbjct: 181 STPHGRKRRSSFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIVLSMIVFFA 240

Query: 241 GSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEI 300
           GSPLYRNKIP+GSPFTTISKVLVAA FGCCIG NSRNSI+SM MSPALDDKEA QN KE 
Sbjct: 241 GSPLYRNKIPNGSPFTTISKVLVAAMFGCCIGMNSRNSISSMEMSPALDDKEAIQNEKER 300

Query: 301 DTTDHMMNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360
            +TD  + EPSESLKFLNNA LNKPF+SALECTVKEVEEVKIVLRILPIFACTIILNCCV
Sbjct: 301 HSTDE-VEEPSESLKFLNNAALNKPFYSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360

Query: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG 420
           AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG
Sbjct: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG 420

Query: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGS 480
           ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATE+GLL+SAQPLPI+FLWIAIQYLFLGS
Sbjct: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATEHGLLNSAQPLPISFLWIAIQYLFLGS 480

Query: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWL 540
           ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSV++SI+NNVTD SDHQPWL
Sbjct: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVVISIINNVTDRSDHQPWL 540

Query: 541 SGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 584
           SGRN+NHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKS Q
Sbjct: 541 SGRNLNHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSGQ 579

BLAST of CsaV3_5G032350 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 6.6e-230
Identity = 403/589 (68.42%), Postives = 488/589 (82.85%), Query Frame = 0

Query: 1   MEIGDDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYL 60
           ME+ ++    +RWEGY DWR + A++GRHGG++AASF LVVE+LENLA+LANASNLV+YL
Sbjct: 1   MEVEEE---VSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYL 60

Query: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQ 120
             YMH SPSKSAN+VT+FMGTAFLLALLGGFLSDAFF+++ I+LISA+I+ LGL +L IQ
Sbjct: 61  REYMHMSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQ 120

Query: 121 AKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180
           A+ PSLMPP CD     +  CEE SG KAAMLF GLYLVALGVGGIKGSL SHGAEQFD+
Sbjct: 121 ARTPSLMPPSCD-----SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDE 180

Query: 181 STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA 240
           STP+GRK+RSTFFNYFVFCL+CG L+AVT VVW+EDNKGWEWGFG++ ++IF+SI++F +
Sbjct: 181 STPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLS 240

Query: 241 GSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEI 300
           GS  YRNKIP GSP TTI KVL+AA+  CC   +S N++ASM++SP+  +    +  KE+
Sbjct: 241 GSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPS--NHCVSKGKKEV 300

Query: 301 DTTDHMMNEPSE-----------SLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPI 360
           ++   +     E           SLK LN A   KP H  LECTV++VE+VKIVL++LPI
Sbjct: 301 ESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPI 360

Query: 361 FACTIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIP 420
           FACTI+LNCC+AQLSTFSV+QA++M+TKIGS K+PPASLPIFP++FIM LAPIYDHLIIP
Sbjct: 361 FACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLIIP 420

Query: 421 FSRRITKTETGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFL 480
           F+R+ TKTETG+THLQRIGVGL+ SI AMAVAA VE KRK VA ++GLLDS + LP+TFL
Sbjct: 421 FARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFL 480

Query: 481 WIAIQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNN 540
           WIA+QYLFLGSADLF+LAG LE+FFTEAP+SMRSLATSLSWASLAMGYYLSSVIVSIVN+
Sbjct: 481 WIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNS 540

Query: 541 VTDHSDHQPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYR 579
           +T  S + PWL G++IN Y L+ FYWLMCVLS  NF+HYLFWAM+YKYR
Sbjct: 541 ITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYR 579

BLAST of CsaV3_5G032350 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 2.6e-210
Identity = 374/571 (65.50%), Postives = 450/571 (78.81%), Query Frame = 0

Query: 7   HGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYLSHYMHF 66
           HG  ++WEGY DWR K ALRGRHGG++AASF L VE+LENLAFLANASNLV+YL ++MH 
Sbjct: 6   HGDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHM 65

Query: 67  SPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQAKMPSL 126
           S ++S++ VT FM TAFLLALLGGFL+DAFF+++ I+LISA+I+ LGL +L IQA+ PSL
Sbjct: 66  SLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSL 125

Query: 127 MPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDSTPQGR 186
           MPPPC   A     CE   G KAA LF GLYLV+LG+GGIKGSLPSHGAEQFD+ TP+GR
Sbjct: 126 MPPPCKSSAALR--CEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGR 185

Query: 187 KRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFAGSPLYR 246
           K+RSTFFNY+VFCLSCG L+AVT VVW+EDNKGWEWGFG++ +SIFLSI+VF  GS  Y+
Sbjct: 186 KQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYK 245

Query: 247 NKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEIDTTDHM 306
           NKIP GSP TTI KVL+AA+   C    S N   S  +    ++K   Q+          
Sbjct: 246 NKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSL--------- 305

Query: 307 MNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCVAQLSTF 366
               + SL  LN A+  K  H  LECTV++VE+VKIVL++LPIF CTI+LNCC+AQLST+
Sbjct: 306 ----TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTY 365

Query: 367 SVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETGITHLQR 426
           SV QA+TM+ KI +F VP ASLP+FP++F++ LAP YDHLIIPF+R++TK+E GITHLQR
Sbjct: 366 SVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQR 425

Query: 427 IGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGSADLFSL 486
           IGVGL+ SI AMAVAA VE KRK+VA E GLLDS + LPITFLWIA+QYLFLGSADLF+L
Sbjct: 426 IGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTL 485

Query: 487 AGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWLSGRNIN 546
           AG LEFFFTEAP+SMRSLATSLSWASLA+GYYLSSV+V IVN VT  +   PWL G  +N
Sbjct: 486 AGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GEKLN 545

Query: 547 HYHLERFYWLMCVLSGLNFVHYLFWAMQYKY 578
              L+ FYWLMCVLS +NF+HYLFWA +YKY
Sbjct: 546 RNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of CsaV3_5G032350 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 672.5 bits (1734), Expect = 4.2e-192
Identity = 357/580 (61.55%), Postives = 427/580 (73.62%), Query Frame = 0

Query: 5   DDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYLSHYM 64
           D+  R + W GYVDWR +PALRGRHGG++AASF LVVEVLENLAFLANASNLV+YLS  M
Sbjct: 33  DEANRLSAWNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSTKM 92

Query: 65  HFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQAKMP 124
            FSPS +AN VT FMGTAF LALLGGFL+DAFFT++HIYL+SAAI+ LGL VL +QA   
Sbjct: 93  GFSPSGAANAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHEH 152

Query: 125 SLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDSTPQ 184
           S  P                       LF GLYLVALGVGGIKGSLP HGAEQFD+ T  
Sbjct: 153 STEP------------------WSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSS 212

Query: 185 GRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFAGSPL 244
           GR++RS FFNYF+F LSCG LIAVT+VVW+EDNKGW +GFG++  +I +S+ VF AGS +
Sbjct: 213 GRRQRSFFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRV 272

Query: 245 YRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEIDTTD 304
           YR K+P GSP TT+ KVL AA +       +   + +       DD   KQN    D  D
Sbjct: 273 YRLKVPSGSPITTLFKVLTAALYAKYKKRRTSRIVVTCHTRNDCDDSVTKQNC---DGDD 332

Query: 305 HMMNEPSESLKFLNNAVLNK-PFHSALECTVKEVEEVKIVLRILPIFACTIILNCCVAQL 364
             +        FL   V  +      L CT ++V++VKIV++ILPIF  TI+LNCC+AQL
Sbjct: 333 GFLG------SFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTIMLNCCLAQL 392

Query: 365 STFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETGITH 424
           STFSV+QASTM+TK+GSF VPPA+LP+FP++F+M LAP Y+HL++P +R+ TKTETGITH
Sbjct: 393 STFSVQQASTMNTKLGSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKSTKTETGITH 452

Query: 425 LQRIGVGLLFSIGAMAVAAAVETKRKRV-----ATENGLLDSAQPLPITFLWIAIQYLFL 484
           LQRIG GL+ SI AMAVAA VETKRK V     +  N    S+ PLPITFLW+AIQY+FL
Sbjct: 453 LQRIGTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITFLWVAIQYVFL 512

Query: 485 GSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQP 544
           GSADLF+LAG +EFFFTEAP++MRSLATSLSWASLAMGYY SSV+VS VN VT  + H P
Sbjct: 513 GSADLFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNFVTGLNHHNP 572

Query: 545 WLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYR 579
           WL G N+N YHLERFYWLMCVLSG+NF+HYLFWA +Y YR
Sbjct: 573 WLLGENLNQYHLERFYWLMCVLSGINFLHYLFWASRYVYR 585

BLAST of CsaV3_5G032350 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 8.6e-105
Identity = 226/574 (39.37%), Postives = 341/574 (59.41%), Query Frame = 0

Query: 11  NRW----EGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYLSHYMHF 70
           N W    E  VDWR +P+   +HGG+ AA F L ++  E +   A  +NL+ Y+ + MHF
Sbjct: 20  NNWELAEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHF 79

Query: 71  SPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQAKMPSL 130
             SK+AN VT+F+GT F+ ALLGG+LSDAF  S+   +I   ++L G  +L +QA +P L
Sbjct: 80  PLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQL 139

Query: 131 MPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDSTPQGR 190
            PP C+   +    CEEA G KA + F  LYLVALG G +K ++ +HGA+QF  S P+  
Sbjct: 140 KPPKCNPLIDQ--TCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQS 199

Query: 191 KRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFAGSPLYR 250
           KR S++FN   F  S G LIA+TL+VWV+ + G + GFG++  ++ + II   +G+  +R
Sbjct: 200 KRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFR 259

Query: 251 NKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPAL-DDKEAKQNAKEIDTTDH 310
           NK P  S FT I+ V+VAA          +  +AS +    L  D     +     T  H
Sbjct: 260 NKRPRRSIFTPIAHVIVAAIL--------KRKLASPSDPRMLHGDHHVANDVVPSSTLPH 319

Query: 311 MMNEPSESLKFLNNAVLNKPFHSALE-----CTVKEVEEVKIVLRILPIFACTIILNCCV 370
                +   +FL+ A +     +  E     CTV +VE+VK ++ ++PIFA TI+ N  +
Sbjct: 320 -----TPRFRFLDKACIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTIL 379

Query: 371 AQLSTFSVEQASTMDTKI-GSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTET 430
           AQL TFSV+Q S+M+T++  SF +PPASL   P + ++FL P+YD  ++PF+R++T   +
Sbjct: 380 AQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNS 439

Query: 431 GITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLG 490
           GI  L RIG+GL  S  +M  AA +E KR+  +  +G +       ++  WI  Q+L  G
Sbjct: 440 GIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGRI-------LSIFWITPQFLIFG 499

Query: 491 SADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHS-DHQP 550
            +++F+  G +EFF+ ++   M S   +L++ S + G+Y SSV+VS+VN +T  S D + 
Sbjct: 500 ISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKG 559

Query: 551 WLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWA 573
           WL   ++N   L+ FYWL+ VLS LNF+ YLFW+
Sbjct: 560 WLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWS 571

BLAST of CsaV3_5G032350 vs. ExPASy Swiss-Prot
Match: Q9LSE8 (Protein NRT1/ PTR FAMILY 4.2 OS=Arabidopsis thaliana OX=3702 GN=NPF4.2 PE=2 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 5.6e-104
Identity = 223/566 (39.40%), Postives = 334/566 (59.01%), Query Frame = 0

Query: 14  EGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYLSHYMHFSPSKSAN 73
           E + DW+ K A+ G+HGGI AAS   VV ++EN+ F+AN  N V Y    MH++P+ +AN
Sbjct: 7   EKFEDWKGKEAIPGKHGGIRAASIVCVVVMMENIVFIANGFNFVKYFMGSMHYTPATAAN 66

Query: 74  NVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQAKMPSLMPPPCDR 133
            VT+FMGT+FLL L GGF++D+F T +  +++   I+L+GL +L  QA  P L+P     
Sbjct: 67  MVTNFMGTSFLLTLFGGFIADSFVTHFTTFIVFCCIELMGLILLTFQAHNPKLLPEK--- 126

Query: 134 QANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDSTPQGRKRRSTFF 193
                   +  S  ++A+LF GLY +A+G GG+K SLPSHG +Q D   P   +  S FF
Sbjct: 127 -------DKTPSTLQSAILFTGLYAMAIGTGGLKASLPSHGGDQIDRRNP---RLISRFF 186

Query: 194 NYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFAGSPLYRNKIPDGS 253
           ++  F +  G L+AVT+V+W+E+ KGW W F I++  +  ++ +F  G P YR K P+GS
Sbjct: 187 DWLYFSICSGCLLAVTVVLWIEEKKGWIWSFNISVGILATALCIFTVGLPFYRFKRPNGS 246

Query: 254 PFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEIDTTDHMMNEPSES 313
           P   I+ V+++A         +RN   S        D+E  +    I       N     
Sbjct: 247 PLKKIAIVIISA---------ARNRNKSDL------DEEMMRGLISI-----YKNNSHNK 306

Query: 314 LKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCVAQLSTFSVEQAST 373
           LK+++ A LNK        +  EVEE +  L +LPIF  TI+++CCVAQLSTFS +Q   
Sbjct: 307 LKWIDKATLNK------NISETEVEETRTFLGLLPIFGSTIVMSCCVAQLSTFSAQQGML 366

Query: 374 MDTKI-GSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRIT---KTETGITHLQRIGV 433
           M+ K+  SF++P  SL   P++F++   P+Y+     F ++I+      +   +L+RIG+
Sbjct: 367 MNKKLFHSFEIPVPSLTAIPLIFMLLSIPLYEF----FGKKISSGNNNRSSSFNLKRIGL 426

Query: 434 GLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGSADLFSLAGS 493
           GL  S  +MAV+A VE KRK     N          I+ LW+  QYL L  +D+ +L G 
Sbjct: 427 GLALSSVSMAVSAIVEAKRKHEVVHNN-------FRISVLWLVFQYLMLSVSDMLTLGGM 486

Query: 494 LEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWLSGRNINHYH 553
           LEFF+ EAP++M+S++T+L W S A+G++LS+ +V + N VT    HQ WL G ++N   
Sbjct: 487 LEFFYREAPSNMKSISTALGWCSTALGFFLSTTLVEVTNAVTGRLGHQ-WLGGEDLNKTR 521

Query: 554 LERFYWLMCVLSGLNFVHYLFWAMQY 576
           LE FY L+CVL+ LN ++Y+FWA +Y
Sbjct: 547 LELFYVLLCVLNTLNLLNYIFWAKRY 521

BLAST of CsaV3_5G032350 vs. ExPASy TrEMBL
Match: E5G6Y7 (Nitrate transporter 1.2a OS=Cucumis sativus OX=3659 GN=Csa_5G604280 PE=2 SV=1)

HSP 1 Score: 1151.7 bits (2978), Expect = 0.0e+00
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 1   MEIGDDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYL 60
           MEIGDDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYL
Sbjct: 1   MEIGDDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYL 60

Query: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQ 120
           SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQ
Sbjct: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQ 120

Query: 121 AKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180
           AKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD
Sbjct: 121 AKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180

Query: 181 STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA 240
           STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA
Sbjct: 181 STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA 240

Query: 241 GSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEI 300
           GSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEI
Sbjct: 241 GSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEI 300

Query: 301 DTTDHMMNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360
           DTTDHMMNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCV
Sbjct: 301 DTTDHMMNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360

Query: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG 420
           AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG
Sbjct: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG 420

Query: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGS 480
           ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGS
Sbjct: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGS 480

Query: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWL 540
           ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWL
Sbjct: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWL 540

Query: 541 SGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 584
           SGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ
Sbjct: 541 SGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 583

BLAST of CsaV3_5G032350 vs. ExPASy TrEMBL
Match: A0A6J1KER1 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita maxima OX=3661 GN=LOC111493153 PE=3 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 3.5e-303
Identity = 534/583 (91.60%), Postives = 556/583 (95.37%), Query Frame = 0

Query: 1   MEIGDDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYL 60
           ME GD   R +RWEGYVDWRK+PAL+GRHGGIIAA+FDLVVE+LENLAFLANASNLVMYL
Sbjct: 1   MESGD---RVSRWEGYVDWRKRPALKGRHGGIIAAAFDLVVEILENLAFLANASNLVMYL 60

Query: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQ 120
           SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSY IY ISAAIQLLGL VLLIQ
Sbjct: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYLIYFISAAIQLLGLAVLLIQ 120

Query: 121 AKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180
           AK+PSLMPPPCDR ANH  +C+EASGGK AMLFAGLYLVALGVGGIKGSLPSHGAEQFDD
Sbjct: 121 AKIPSLMPPPCDRHANHGTMCQEASGGKEAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180

Query: 181 STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA 240
           STP GRKRRS+FFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSI LS+IVFFA
Sbjct: 181 STPHGRKRRSSFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIVLSMIVFFA 240

Query: 241 GSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEI 300
           GSPLYRNKIP+GSPFTTISKVLVAA FGCCIG NSRNSI+SM MSPALDDKEA QN KE 
Sbjct: 241 GSPLYRNKIPNGSPFTTISKVLVAAMFGCCIGMNSRNSISSMEMSPALDDKEAIQNEKER 300

Query: 301 DTTDHMMNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360
            +TD  + EPSESLKFLNNA LNKPF+SALECTVKEVEEVKIVLRILPIFACTIILNCCV
Sbjct: 301 HSTDE-VEEPSESLKFLNNAALNKPFYSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360

Query: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG 420
           AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG
Sbjct: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG 420

Query: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGS 480
           ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATE+GLL+SAQPLPI+FLWIAIQYLFLGS
Sbjct: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATEHGLLNSAQPLPISFLWIAIQYLFLGS 480

Query: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWL 540
           ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSV++SI+NNVTD SDHQPWL
Sbjct: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVVISIINNVTDRSDHQPWL 540

Query: 541 SGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 584
           SGRN+NHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKS Q
Sbjct: 541 SGRNLNHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSGQ 579

BLAST of CsaV3_5G032350 vs. ExPASy TrEMBL
Match: A0A6J1GYW3 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111458419 PE=3 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 6.7e-302
Identity = 531/583 (91.08%), Postives = 557/583 (95.54%), Query Frame = 0

Query: 1   MEIGDDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYL 60
           ME GD   R +RWEGYVDWRK+PAL+GRHGGIIAA+FDLVVE+LENLAFLANASNLVMYL
Sbjct: 1   MESGD---RVSRWEGYVDWRKRPALKGRHGGIIAAAFDLVVEILENLAFLANASNLVMYL 60

Query: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQ 120
           SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSY IYLISAAIQLLGL VLLIQ
Sbjct: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYLIYLISAAIQLLGLAVLLIQ 120

Query: 121 AKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180
           AK+PSLMPPPC R ANH  +C+EASGGK AMLFAGLYLVALGVGGIKGSLPSHGAEQFDD
Sbjct: 121 AKIPSLMPPPCYRHANHGTMCQEASGGKEAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180

Query: 181 STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA 240
           STP GRKRRS+FFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSI LS+IVFFA
Sbjct: 181 STPHGRKRRSSFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIVLSMIVFFA 240

Query: 241 GSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEI 300
           GSPLYRNKIP+GSPFTTISKVLVAA FGCCIG NSRNSI+SM MSPALDDKEA QN KE 
Sbjct: 241 GSPLYRNKIPNGSPFTTISKVLVAAMFGCCIGMNSRNSISSMEMSPALDDKEAIQNEKER 300

Query: 301 DTTDHMMNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360
            +TD  + EPSESLKFLNNA LNKPF+SALECTVKEVEEVKIVLRILPIFACTIILNCCV
Sbjct: 301 HSTDE-VEEPSESLKFLNNAALNKPFYSALECTVKEVEEVKIVLRILPIFACTIILNCCV 360

Query: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETG 420
           AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDH+IIPFSRRITKTETG
Sbjct: 361 AQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIIIPFSRRITKTETG 420

Query: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGS 480
           ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVAT++GLL+SAQPLPI+FLWIAIQYLFLGS
Sbjct: 421 ITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATKHGLLNSAQPLPISFLWIAIQYLFLGS 480

Query: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWL 540
           ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSV++SI+NNVTD SDHQPWL
Sbjct: 481 ADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVVISIINNVTDRSDHQPWL 540

Query: 541 SGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 584
           SGRN+NHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPK++Q
Sbjct: 541 SGRNLNHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKARQ 579

BLAST of CsaV3_5G032350 vs. ExPASy TrEMBL
Match: A0A6J1HTC9 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita maxima OX=3661 GN=LOC111467635 PE=3 SV=1)

HSP 1 Score: 1039.6 bits (2687), Expect = 4.8e-300
Identity = 526/572 (91.96%), Postives = 551/572 (96.33%), Query Frame = 0

Query: 12  RWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYLSHYMHFSPSKS 71
           RWEGYVDWRK+ A+RGRHGG+IAASFDLVVE+LENLAFLANASNLVMYLSHYMHFSPSKS
Sbjct: 8   RWEGYVDWRKRAAVRGRHGGMIAASFDLVVEILENLAFLANASNLVMYLSHYMHFSPSKS 67

Query: 72  ANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQAKMPSLMPPPC 131
           ANNVT FMGTAFLLALLGGFLSDAFFTSY I+LISAAIQLLGL +L+IQAKMPSL PPPC
Sbjct: 68  ANNVTAFMGTAFLLALLGGFLSDAFFTSYLIFLISAAIQLLGLAILVIQAKMPSLKPPPC 127

Query: 132 DRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDSTPQGRKRRST 191
           D QANH A+C+EASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDS PQGRKRRST
Sbjct: 128 DHQANHTAMCQEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDSNPQGRKRRST 187

Query: 192 FFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFAGSPLYRNKIPD 251
           FFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGI+MLSIFLS++VFFAGSPLYRNKIP+
Sbjct: 188 FFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGISMLSIFLSMVVFFAGSPLYRNKIPN 247

Query: 252 GSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEIDTTDHMMNEPS 311
           GSPFTTISKVLVAATFGCC+  NSRNSIASMAMSPALD KEAKQNAK  D+TD +++EPS
Sbjct: 248 GSPFTTISKVLVAATFGCCVSPNSRNSIASMAMSPALDGKEAKQNAKGRDSTD-VVDEPS 307

Query: 312 ESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCVAQLSTFSVEQA 371
           ESLKFLNNAVL KPFHSALECTVKEVEEVKIVL+ILPIFACTIILNCCVAQLSTFSVEQA
Sbjct: 308 ESLKFLNNAVLKKPFHSALECTVKEVEEVKIVLKILPIFACTIILNCCVAQLSTFSVEQA 367

Query: 372 STMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETGITHLQRIGVGL 431
           STMDTKIGSFKVPPASLPIFPILFIMFLAPIYDH++IPF+RRITKTE GITHLQRIGVGL
Sbjct: 368 STMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIVIPFARRITKTEMGITHLQRIGVGL 427

Query: 432 LFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGSADLFSLAGSLE 491
           +FSIGAMAVAAAVETKRKRVAT NGLLDSAQPLPITFLWIAIQYLFLGSADLFSLAGSLE
Sbjct: 428 VFSIGAMAVAAAVETKRKRVATGNGLLDSAQPLPITFLWIAIQYLFLGSADLFSLAGSLE 487

Query: 492 FFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWLSGRNINHYHLE 551
           FFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVN VT +S+H PWLSG NINHYHLE
Sbjct: 488 FFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNTVTGNSNHHPWLSGTNINHYHLE 547

Query: 552 RFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 584
           RFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ
Sbjct: 548 RFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 578

BLAST of CsaV3_5G032350 vs. ExPASy TrEMBL
Match: A0A6J1FZJ7 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449346 PE=3 SV=1)

HSP 1 Score: 1033.9 bits (2672), Expect = 2.6e-298
Identity = 523/572 (91.43%), Postives = 549/572 (95.98%), Query Frame = 0

Query: 12  RWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYLSHYMHFSPSKS 71
           RWEGYVDWRK+ A+RGRHGG+IAASFDLVVE+LENLAFLANASNLVMYLSHYMHFSPSKS
Sbjct: 8   RWEGYVDWRKRAAVRGRHGGMIAASFDLVVEILENLAFLANASNLVMYLSHYMHFSPSKS 67

Query: 72  ANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQAKMPSLMPPPC 131
           ANNVT FMGTAFLLALLGGFLSDAFFTSY I+LISAAIQLLGL +LLIQAKMPSL PPPC
Sbjct: 68  ANNVTAFMGTAFLLALLGGFLSDAFFTSYLIFLISAAIQLLGLAILLIQAKMPSLKPPPC 127

Query: 132 DRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDSTPQGRKRRST 191
           D QANH A+C+EASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDS PQGRKRRST
Sbjct: 128 DHQANHTAMCQEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDSNPQGRKRRST 187

Query: 192 FFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFAGSPLYRNKIPD 251
           FFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGI+MLSIFLS++VFFAGSPLYRNKIP+
Sbjct: 188 FFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGISMLSIFLSMVVFFAGSPLYRNKIPN 247

Query: 252 GSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEIDTTDHMMNEPS 311
           GSPFTTISKVLVAATF CC+  NS+NSIASMAMSPALD KEAKQNAK  D+TD +++EPS
Sbjct: 248 GSPFTTISKVLVAATFSCCVSPNSQNSIASMAMSPALDGKEAKQNAKGRDSTD-VVDEPS 307

Query: 312 ESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCVAQLSTFSVEQA 371
           ESLKFLNNAVL KPFHSALECTVKEVEEVKIVL+ILPIF CTIILNCCVAQLSTFSVEQA
Sbjct: 308 ESLKFLNNAVLKKPFHSALECTVKEVEEVKIVLKILPIFGCTIILNCCVAQLSTFSVEQA 367

Query: 372 STMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETGITHLQRIGVGL 431
           STMDTKIGSFKVPPASLPIFPILFIMFLAP+YDH+IIPF+RRITKTE GITHLQRIGVGL
Sbjct: 368 STMDTKIGSFKVPPASLPIFPILFIMFLAPVYDHIIIPFARRITKTEMGITHLQRIGVGL 427

Query: 432 LFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGSADLFSLAGSLE 491
           +FSIGAMAVAAAVETKRK VAT+NGLLDSAQPLPITFLWIAIQYLFLGSADLFSLAGSLE
Sbjct: 428 VFSIGAMAVAAAVETKRKGVATKNGLLDSAQPLPITFLWIAIQYLFLGSADLFSLAGSLE 487

Query: 492 FFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWLSGRNINHYHLE 551
           FFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVN VT +S+H PWLSG NINHYHLE
Sbjct: 488 FFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNTVTGNSNHHPWLSGTNINHYHLE 547

Query: 552 RFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 584
           RFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ
Sbjct: 548 RFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 578

BLAST of CsaV3_5G032350 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 798.1 bits (2060), Expect = 4.7e-231
Identity = 403/589 (68.42%), Postives = 488/589 (82.85%), Query Frame = 0

Query: 1   MEIGDDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYL 60
           ME+ ++    +RWEGY DWR + A++GRHGG++AASF LVVE+LENLA+LANASNLV+YL
Sbjct: 1   MEVEEE---VSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYL 60

Query: 61  SHYMHFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQ 120
             YMH SPSKSAN+VT+FMGTAFLLALLGGFLSDAFF+++ I+LISA+I+ LGL +L IQ
Sbjct: 61  REYMHMSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQ 120

Query: 121 AKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDD 180
           A+ PSLMPP CD     +  CEE SG KAAMLF GLYLVALGVGGIKGSL SHGAEQFD+
Sbjct: 121 ARTPSLMPPSCD-----SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDE 180

Query: 181 STPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFA 240
           STP+GRK+RSTFFNYFVFCL+CG L+AVT VVW+EDNKGWEWGFG++ ++IF+SI++F +
Sbjct: 181 STPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLS 240

Query: 241 GSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEI 300
           GS  YRNKIP GSP TTI KVL+AA+  CC   +S N++ASM++SP+  +    +  KE+
Sbjct: 241 GSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPS--NHCVSKGKKEV 300

Query: 301 DTTDHMMNEPSE-----------SLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPI 360
           ++   +     E           SLK LN A   KP H  LECTV++VE+VKIVL++LPI
Sbjct: 301 ESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPI 360

Query: 361 FACTIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIP 420
           FACTI+LNCC+AQLSTFSV+QA++M+TKIGS K+PPASLPIFP++FIM LAPIYDHLIIP
Sbjct: 361 FACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLIIP 420

Query: 421 FSRRITKTETGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFL 480
           F+R+ TKTETG+THLQRIGVGL+ SI AMAVAA VE KRK VA ++GLLDS + LP+TFL
Sbjct: 421 FARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFL 480

Query: 481 WIAIQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNN 540
           WIA+QYLFLGSADLF+LAG LE+FFTEAP+SMRSLATSLSWASLAMGYYLSSVIVSIVN+
Sbjct: 481 WIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNS 540

Query: 541 VTDHSDHQPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYR 579
           +T  S + PWL G++IN Y L+ FYWLMCVLS  NF+HYLFWAM+YKYR
Sbjct: 541 ITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYR 579

BLAST of CsaV3_5G032350 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 733.0 bits (1891), Expect = 1.8e-211
Identity = 374/571 (65.50%), Postives = 450/571 (78.81%), Query Frame = 0

Query: 7   HGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYLSHYMHF 66
           HG  ++WEGY DWR K ALRGRHGG++AASF L VE+LENLAFLANASNLV+YL ++MH 
Sbjct: 2   HGDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHM 61

Query: 67  SPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQAKMPSL 126
           S ++S++ VT FM TAFLLALLGGFL+DAFF+++ I+LISA+I+ LGL +L IQA+ PSL
Sbjct: 62  SLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSL 121

Query: 127 MPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDSTPQGR 186
           MPPPC   A     CE   G KAA LF GLYLV+LG+GGIKGSLPSHGAEQFD+ TP+GR
Sbjct: 122 MPPPCKSSAALR--CEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGR 181

Query: 187 KRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFAGSPLYR 246
           K+RSTFFNY+VFCLSCG L+AVT VVW+EDNKGWEWGFG++ +SIFLSI+VF  GS  Y+
Sbjct: 182 KQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYK 241

Query: 247 NKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEIDTTDHM 306
           NKIP GSP TTI KVL+AA+   C    S N   S  +    ++K   Q+          
Sbjct: 242 NKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSL--------- 301

Query: 307 MNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCVAQLSTF 366
               + SL  LN A+  K  H  LECTV++VE+VKIVL++LPIF CTI+LNCC+AQLST+
Sbjct: 302 ----TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTY 361

Query: 367 SVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETGITHLQR 426
           SV QA+TM+ KI +F VP ASLP+FP++F++ LAP YDHLIIPF+R++TK+E GITHLQR
Sbjct: 362 SVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQR 421

Query: 427 IGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGSADLFSL 486
           IGVGL+ SI AMAVAA VE KRK+VA E GLLDS + LPITFLWIA+QYLFLGSADLF+L
Sbjct: 422 IGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTL 481

Query: 487 AGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWLSGRNIN 546
           AG LEFFFTEAP+SMRSLATSLSWASLA+GYYLSSV+V IVN VT  +   PWL G  +N
Sbjct: 482 AGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GEKLN 541

Query: 547 HYHLERFYWLMCVLSGLNFVHYLFWAMQYKY 578
              L+ FYWLMCVLS +NF+HYLFWA +YKY
Sbjct: 542 RNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 556

BLAST of CsaV3_5G032350 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 733.0 bits (1891), Expect = 1.8e-211
Identity = 374/571 (65.50%), Postives = 450/571 (78.81%), Query Frame = 0

Query: 7   HGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYLSHYMHF 66
           HG  ++WEGY DWR K ALRGRHGG++AASF L VE+LENLAFLANASNLV+YL ++MH 
Sbjct: 6   HGDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHM 65

Query: 67  SPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQAKMPSL 126
           S ++S++ VT FM TAFLLALLGGFL+DAFF+++ I+LISA+I+ LGL +L IQA+ PSL
Sbjct: 66  SLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSL 125

Query: 127 MPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDSTPQGR 186
           MPPPC   A     CE   G KAA LF GLYLV+LG+GGIKGSLPSHGAEQFD+ TP+GR
Sbjct: 126 MPPPCKSSAALR--CEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGR 185

Query: 187 KRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFAGSPLYR 246
           K+RSTFFNY+VFCLSCG L+AVT VVW+EDNKGWEWGFG++ +SIFLSI+VF  GS  Y+
Sbjct: 186 KQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYK 245

Query: 247 NKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEIDTTDHM 306
           NKIP GSP TTI KVL+AA+   C    S N   S  +    ++K   Q+          
Sbjct: 246 NKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSL--------- 305

Query: 307 MNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCVAQLSTF 366
               + SL  LN A+  K  H  LECTV++VE+VKIVL++LPIF CTI+LNCC+AQLST+
Sbjct: 306 ----TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTY 365

Query: 367 SVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETGITHLQR 426
           SV QA+TM+ KI +F VP ASLP+FP++F++ LAP YDHLIIPF+R++TK+E GITHLQR
Sbjct: 366 SVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQR 425

Query: 427 IGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGSADLFSL 486
           IGVGL+ SI AMAVAA VE KRK+VA E GLLDS + LPITFLWIA+QYLFLGSADLF+L
Sbjct: 426 IGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTL 485

Query: 487 AGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQPWLSGRNIN 546
           AG LEFFFTEAP+SMRSLATSLSWASLA+GYYLSSV+V IVN VT  +   PWL G  +N
Sbjct: 486 AGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GEKLN 545

Query: 547 HYHLERFYWLMCVLSGLNFVHYLFWAMQYKY 578
              L+ FYWLMCVLS +NF+HYLFWA +YKY
Sbjct: 546 RNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of CsaV3_5G032350 vs. TAIR 10
Match: AT5G62730.1 (Major facilitator superfamily protein )

HSP 1 Score: 672.5 bits (1734), Expect = 3.0e-193
Identity = 357/580 (61.55%), Postives = 427/580 (73.62%), Query Frame = 0

Query: 5   DDHGRTNRWEGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYLSHYM 64
           D+  R + W GYVDWR +PALRGRHGG++AASF LVVEVLENLAFLANASNLV+YLS  M
Sbjct: 33  DEANRLSAWNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSTKM 92

Query: 65  HFSPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQAKMP 124
            FSPS +AN VT FMGTAF LALLGGFL+DAFFT++HIYL+SAAI+ LGL VL +QA   
Sbjct: 93  GFSPSGAANAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHEH 152

Query: 125 SLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDSTPQ 184
           S  P                       LF GLYLVALGVGGIKGSLP HGAEQFD+ T  
Sbjct: 153 STEP------------------WSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSS 212

Query: 185 GRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFAGSPL 244
           GR++RS FFNYF+F LSCG LIAVT+VVW+EDNKGW +GFG++  +I +S+ VF AGS +
Sbjct: 213 GRRQRSFFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRV 272

Query: 245 YRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKEAKQNAKEIDTTD 304
           YR K+P GSP TT+ KVL AA +       +   + +       DD   KQN    D  D
Sbjct: 273 YRLKVPSGSPITTLFKVLTAALYAKYKKRRTSRIVVTCHTRNDCDDSVTKQNC---DGDD 332

Query: 305 HMMNEPSESLKFLNNAVLNK-PFHSALECTVKEVEEVKIVLRILPIFACTIILNCCVAQL 364
             +        FL   V  +      L CT ++V++VKIV++ILPIF  TI+LNCC+AQL
Sbjct: 333 GFLG------SFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTIMLNCCLAQL 392

Query: 365 STFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETGITH 424
           STFSV+QASTM+TK+GSF VPPA+LP+FP++F+M LAP Y+HL++P +R+ TKTETGITH
Sbjct: 393 STFSVQQASTMNTKLGSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKSTKTETGITH 452

Query: 425 LQRIGVGLLFSIGAMAVAAAVETKRKRV-----ATENGLLDSAQPLPITFLWIAIQYLFL 484
           LQRIG GL+ SI AMAVAA VETKRK V     +  N    S+ PLPITFLW+AIQY+FL
Sbjct: 453 LQRIGTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITFLWVAIQYVFL 512

Query: 485 GSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHSDHQP 544
           GSADLF+LAG +EFFFTEAP++MRSLATSLSWASLAMGYY SSV+VS VN VT  + H P
Sbjct: 513 GSADLFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNFVTGLNHHNP 572

Query: 545 WLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYR 579
           WL G N+N YHLERFYWLMCVLSG+NF+HYLFWA +Y YR
Sbjct: 573 WLLGENLNQYHLERFYWLMCVLSGINFLHYLFWASRYVYR 585

BLAST of CsaV3_5G032350 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 382.5 bits (981), Expect = 6.1e-106
Identity = 226/574 (39.37%), Postives = 341/574 (59.41%), Query Frame = 0

Query: 11  NRW----EGYVDWRKKPALRGRHGGIIAASFDLVVEVLENLAFLANASNLVMYLSHYMHF 70
           N W    E  VDWR +P+   +HGG+ AA F L ++  E +   A  +NL+ Y+ + MHF
Sbjct: 20  NNWELAEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHF 79

Query: 71  SPSKSANNVTDFMGTAFLLALLGGFLSDAFFTSYHIYLISAAIQLLGLTVLLIQAKMPSL 130
             SK+AN VT+F+GT F+ ALLGG+LSDAF  S+   +I   ++L G  +L +QA +P L
Sbjct: 80  PLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQL 139

Query: 131 MPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDSTPQGR 190
            PP C+   +    CEEA G KA + F  LYLVALG G +K ++ +HGA+QF  S P+  
Sbjct: 140 KPPKCNPLIDQ--TCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQS 199

Query: 191 KRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIIVFFAGSPLYR 250
           KR S++FN   F  S G LIA+TL+VWV+ + G + GFG++  ++ + II   +G+  +R
Sbjct: 200 KRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFR 259

Query: 251 NKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPAL-DDKEAKQNAKEIDTTDH 310
           NK P  S FT I+ V+VAA          +  +AS +    L  D     +     T  H
Sbjct: 260 NKRPRRSIFTPIAHVIVAAIL--------KRKLASPSDPRMLHGDHHVANDVVPSSTLPH 319

Query: 311 MMNEPSESLKFLNNAVLNKPFHSALE-----CTVKEVEEVKIVLRILPIFACTIILNCCV 370
                +   +FL+ A +     +  E     CTV +VE+VK ++ ++PIFA TI+ N  +
Sbjct: 320 -----TPRFRFLDKACIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTIL 379

Query: 371 AQLSTFSVEQASTMDTKI-GSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTET 430
           AQL TFSV+Q S+M+T++  SF +PPASL   P + ++FL P+YD  ++PF+R++T   +
Sbjct: 380 AQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNS 439

Query: 431 GITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLG 490
           GI  L RIG+GL  S  +M  AA +E KR+  +  +G +       ++  WI  Q+L  G
Sbjct: 440 GIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGRI-------LSIFWITPQFLIFG 499

Query: 491 SADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDHS-DHQP 550
            +++F+  G +EFF+ ++   M S   +L++ S + G+Y SSV+VS+VN +T  S D + 
Sbjct: 500 ISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKG 559

Query: 551 WLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWA 573
           WL   ++N   L+ FYWL+ VLS LNF+ YLFW+
Sbjct: 560 WLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWS 571

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011655668.10.0e+00100.00protein NRT1/ PTR FAMILY 4.6 [Cucumis sativus] >ADQ74764.1 nitrate transporter 1... [more]
XP_038893294.10.0e+0096.23protein NRT1/ PTR FAMILY 4.6-like [Benincasa hispida][more]
XP_023537771.12.5e-30391.42protein NRT1/ PTR FAMILY 4.6-like [Cucurbita pepo subsp. pepo][more]
KAG6601408.13.3e-30391.42Protein NRT1/ PTR FAMILY 4.6, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022998594.17.3e-30391.60protein NRT1/ PTR FAMILY 4.6-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8H1576.6e-23068.42Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q8VYE42.6e-21065.50Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q9FM204.2e-19261.55Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2[more]
Q93VV58.6e-10539.37Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Q9LSE85.6e-10439.40Protein NRT1/ PTR FAMILY 4.2 OS=Arabidopsis thaliana OX=3702 GN=NPF4.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
E5G6Y70.0e+00100.00Nitrate transporter 1.2a OS=Cucumis sativus OX=3659 GN=Csa_5G604280 PE=2 SV=1[more]
A0A6J1KER13.5e-30391.60protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita maxima OX=3661 GN=LOC111493153 PE... [more]
A0A6J1GYW36.7e-30291.08protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111458419 ... [more]
A0A6J1HTC94.8e-30091.96protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita maxima OX=3661 GN=LOC111467635 PE... [more]
A0A6J1FZJ72.6e-29891.43protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449346 ... [more]
Match NameE-valueIdentityDescription
AT1G69850.14.7e-23168.42nitrate transporter 1:2 [more]
AT1G27040.21.8e-21165.50Major facilitator superfamily protein [more]
AT1G27040.11.8e-21165.50Major facilitator superfamily protein [more]
AT5G62730.13.0e-19361.55Major facilitator superfamily protein [more]
AT1G59740.16.1e-10639.37Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 104..535
e-value: 2.4E-107
score: 359.2
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 15..580
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 5..583
e-value: 6.9E-171
score: 571.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 51..579
NoneNo IPR availablePANTHERPTHR11654:SF490PROTEIN NRT1/ PTR FAMILY 4.6-LIKEcoord: 15..580
NoneNo IPR availableCDDcd17414MFS_NPF4coord: 32..573
e-value: 0.0
score: 657.56

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_5G032350.1CsaV3_5G032350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity